AMBRA1 cDNA ORF clone, Homo sapiens(human)

The following AMBRA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMBRA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu69547 XM_024448606.1
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu107382 NM_001367470.1
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu92368 XM_017018007.1
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92369 XM_017018008.1
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu09585 NM_001367471.1
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $69.30
$99.00
OHu09585 NM_017749.3
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $69.30
$99.00
OHu09779 NM_001267783.2
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu12050 NM_001267782.2
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu44914 NM_001367468.1
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu44916 NM_001300731.2
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu44917 NM_001367469.1
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu09585 XM_005253014.3
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $69.30
$99.00
OHu44914 XM_005253009.4
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu44914 XM_006718259.2
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu44914 XM_024448605.1
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu44915 XM_005253011.4
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu44917 XM_006718260.2
Latest version!
Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu09585 XM_005253014.2 Homo sapiens autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $69.30
$99.00
OHu12050 NM_001267782.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu44916 NM_001300731.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu44914 XM_005253009.3 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu09779 NM_001267783.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu44915 XM_005253011.3 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu69547
    Clone ID Related Accession (Same CDS sequence) XM_024448606.1
    Accession Version XM_024448606.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2805bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG
    AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG
    GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC
    AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC
    CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC
    CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC
    ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC
    ATGAGGGTCT TGGTTGCCCC TTACGTCCAA AGGCACCAGA TCACTCAGCA AATGTCCCTG
    AATTCAGTGA TTGGTGGAAG GGAAGACACC AGCTTTTTTG GATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024304374.1
    CDS349..3153
    Translation

    Target ORF information:

    RefSeq Version XM_024448606.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024448606.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG
    AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG
    GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC
    AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC
    CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC
    CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC
    ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC
    ATGAGGGTCT TGGTTGCCCC TTACGTCCAA AGGCACCAGA TCACTCAGCA AATGTCCCTG
    AATTCAGTGA TTGGTGGAAG GGAAGACACC AGCTTTTTTG GATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107382
    Clone ID Related Accession (Same CDS sequence) NM_001367470.1
    Accession Version NM_001367470.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform 7
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127035.6, AC115097.6, AC024293.18 and AC116021.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.176312.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAACGGG TCCGTCTGGT GAGATTTGAT CCACTTGGAC ACTACTTACT CACAGCAATT 
    GTTAACCCCT CTAATCAACA GGGTGATGAC GAACCAGAGA TCCCCATAGA TGGAACAGAA
    TTATCCCACT ACCGTCAGCG TGCCCTCCTG CAATCACAGC CAGTTCGCCG GACGCCTCTC
    CTCCACAATT TCCTGCACAT GCTGTCCTCC CGCTCTTCTG GCATCCAGAC CGAGCCCTTC
    CATCCCCCGG AGCAGGCCTC GTCAACGCAG CAGGACCAGG GCCTCCTGAA CCGGCCGTCT
    GCCTTCAGTA CAGTCCAGAG CAGCACTGCC GGCAACACGC TCCGCAACCT CAGTCTGGGT
    CCTACCCGCC GCTCTTTGGG AGGGCCTCTG TCTAGCCACC CTTCTAGGTA TCACCGAGAA
    ATAGCTCCTG GGTTGACAGG ATCTGAGTGG ACCCGGACAG TACTCAGTCT GAACTCCCGC
    TCTGAGGCGG AATCCATGCC CCCGCCCAGA ACCAGTGCCT CTTCGGTGAG TTTGCTGTCT
    GTGCTGAGAC AGCAGGAAGG TGGCTCTCAG GCATCTGTGT ACACTTCAGC CACAGAAGGG
    AGGGGTTTTC CGGCATCAGG GTTGGCAACT GAGTCAGATG GAGGGAATGG CTCCAGCCAA
    AACAACTCGG GCAGCATTCG CCATGAGCTT CAGTGTGACC TGAGACGCTT CTTTCTGGAG
    TATGACCGGC TTCAGGAGCT GGATCAGAGC CTGAGTGGGG AAGCTCCCCA GACCCAACAG
    GCCCAGGAAA TGCTCAACAA TAACATTGAA TCTGAGAGGC CAGGCCCTTC CCACCAGCCC
    ACCCCACACA GCAGTGAGAA CAACTCCAAC CTGTCCCGTG GCCACCTGAA TCGCTGTCGT
    GCTTGCCACA ATCTCCTGAC CTTCAACAAC GATACCCTGC GCTGGGAAAG AACCACACCT
    AACTACTCCT CTGGCGAGGC TAGTTCCTCT TGGCAGGTCC CCAGCTCCTT TGAGAGTGTG
    CCATCAAGTG GCAGCCAGTT GCCACCTCTC GAGCGGACTG AGGGCCAAAC GCCCAGCTCC
    AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT CCGCAGGAGG AGAGGACTGT GGGGGTGGCC
    TTTAACCAGG AGACAGGCCA CTGGGAAAGA ATTTACACCC AGTCCAGCAG ATCTGGAACT
    GTGTCACAGG AGGCCTTACA TCAGGATATG CCTGAGGAGA GCTCTGAGGA GGATTCACTC
    AGGAGGAGGC TGCTGGAATC TTCCCTCATT TCATTATCCC GTTATGATGG AGCAGGATCC
    AGAGAGCACC CAATTTACCC AGACCCAGCG AGATTATCTC CTGCTGCATA CTACGCCCAG
    AGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTACCAA
    CAGAACCGTC TCCGTTCTTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCAGTA
    GAGGGAACCG ACTTGGAATT TGAGGACTTT GAGGACAATG GTGACAGATC CAGGCACCGA
    GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC
    TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG
    GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT
    GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG
    TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC
    TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC
    AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT
    AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG
    TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC
    TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC
    TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC
    AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC
    AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG
    GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT
    GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG
    CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT
    CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC
    TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC
    AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC
    ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC
    CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC
    ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG
    ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC
    TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC
    TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA
    GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001354399.1
    CDS843..3932
    Translation

    Target ORF information:

    RefSeq Version NM_001367470.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001367470.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAACGGG TCCGTCTGGT GAGATTTGAT CCACTTGGAC ACTACTTACT CACAGCAATT 
    GTTAACCCCT CTAATCAACA GGGTGATGAC GAACCAGAGA TCCCCATAGA TGGAACAGAA
    TTATCCCACT ACCGTCAGCG TGCCCTCCTG CAATCACAGC CAGTTCGCCG GACGCCTCTC
    CTCCACAATT TCCTGCACAT GCTGTCCTCC CGCTCTTCTG GCATCCAGAC CGAGCCCTTC
    CATCCCCCGG AGCAGGCCTC GTCAACGCAG CAGGACCAGG GCCTCCTGAA CCGGCCGTCT
    GCCTTCAGTA CAGTCCAGAG CAGCACTGCC GGCAACACGC TCCGCAACCT CAGTCTGGGT
    CCTACCCGCC GCTCTTTGGG AGGGCCTCTG TCTAGCCACC CTTCTAGGTA TCACCGAGAA
    ATAGCTCCTG GGTTGACAGG ATCTGAGTGG ACCCGGACAG TACTCAGTCT GAACTCCCGC
    TCTGAGGCGG AATCCATGCC CCCGCCCAGA ACCAGTGCCT CTTCGGTGAG TTTGCTGTCT
    GTGCTGAGAC AGCAGGAAGG TGGCTCTCAG GCATCTGTGT ACACTTCAGC CACAGAAGGG
    AGGGGTTTTC CGGCATCAGG GTTGGCAACT GAGTCAGATG GAGGGAATGG CTCCAGCCAA
    AACAACTCGG GCAGCATTCG CCATGAGCTT CAGTGTGACC TGAGACGCTT CTTTCTGGAG
    TATGACCGGC TTCAGGAGCT GGATCAGAGC CTGAGTGGGG AAGCTCCCCA GACCCAACAG
    GCCCAGGAAA TGCTCAACAA TAACATTGAA TCTGAGAGGC CAGGCCCTTC CCACCAGCCC
    ACCCCACACA GCAGTGAGAA CAACTCCAAC CTGTCCCGTG GCCACCTGAA TCGCTGTCGT
    GCTTGCCACA ATCTCCTGAC CTTCAACAAC GATACCCTGC GCTGGGAAAG AACCACACCT
    AACTACTCCT CTGGCGAGGC TAGTTCCTCT TGGCAGGTCC CCAGCTCCTT TGAGAGTGTG
    CCATCAAGTG GCAGCCAGTT GCCACCTCTC GAGCGGACTG AGGGCCAAAC GCCCAGCTCC
    AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT CCGCAGGAGG AGAGGACTGT GGGGGTGGCC
    TTTAACCAGG AGACAGGCCA CTGGGAAAGA ATTTACACCC AGTCCAGCAG ATCTGGAACT
    GTGTCACAGG AGGCCTTACA TCAGGATATG CCTGAGGAGA GCTCTGAGGA GGATTCACTC
    AGGAGGAGGC TGCTGGAATC TTCCCTCATT TCATTATCCC GTTATGATGG AGCAGGATCC
    AGAGAGCACC CAATTTACCC AGACCCAGCG AGATTATCTC CTGCTGCATA CTACGCCCAG
    AGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTACCAA
    CAGAACCGTC TCCGTTCTTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCAGTA
    GAGGGAACCG ACTTGGAATT TGAGGACTTT GAGGACAATG GTGACAGATC CAGGCACCGA
    GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC
    TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG
    GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT
    GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG
    TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC
    TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC
    AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT
    AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG
    TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC
    TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC
    TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC
    AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC
    AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG
    GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT
    GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG
    CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT
    CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC
    TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC
    AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC
    ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC
    CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC
    ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG
    ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC
    TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC
    TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA
    GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92368
    Clone ID Related Accession (Same CDS sequence) XM_017018007.1
    Accession Version XM_017018007.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGGACAATG GTGACAGATC CAGGCACCGA
    GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC
    TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG
    GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT
    GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG
    TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC
    TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC
    AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT
    AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG
    TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC
    TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC
    TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC
    AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC
    AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG
    GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT
    GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG
    CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT
    CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC
    TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC
    AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC
    ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC
    CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC
    ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG
    ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC
    TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC
    TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA
    GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873496.1
    CDS359..3988
    Translation

    Target ORF information:

    RefSeq Version XM_017018007.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGGACAATG GTGACAGATC CAGGCACCGA
    GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC
    TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG
    GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT
    GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG
    TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC
    TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC
    AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT
    AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG
    TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC
    TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC
    TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC
    AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC
    AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG
    GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT
    GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG
    CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT
    CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC
    TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC
    AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC
    ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC
    CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC
    ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG
    ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC
    TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC
    TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA
    GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92369
    Clone ID Related Accession (Same CDS sequence) XM_017018008.1
    Accession Version XM_017018008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3075bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT
    TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGGTCT TGGTTGCCCC TTACGTCCAA
    AGGCACCAGA TCACTCAGCA AATGTCCCTG AATTCAGTGA TTGGTGGAAG GGAAGACACC
    AGCTTTTTTG GATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873497.1
    CDS359..3433
    Translation

    Target ORF information:

    RefSeq Version XM_017018008.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT
    TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGGTCT TGGTTGCCCC TTACGTCCAA
    AGGCACCAGA TCACTCAGCA AATGTCCCTG AATTCAGTGA TTGGTGGAAG GGAAGACACC
    AGCTTTTTTG GATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09585
    Clone ID Related Accession (Same CDS sequence) XM_005253014.2 , NM_001367471.1 , XM_005253014.3 , NM_017749.3
    Accession Version NM_001367471.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127035.6, AC115097.6, AC024293.18 and AC116021.5. On Dec 1, 2018 this sequence version replaced XM_005253014.4. Transcript Variant: This variant (8), as well as variant 2, encodes isoform 2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.197066.1, SRR1660807.82204.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG
    AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG
    GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC
    AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC
    CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC
    CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC
    ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC
    ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT
    ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA
    GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC
    CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA
    ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT
    CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG
    CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA
    GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG
    ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC
    ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC
    AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC
    TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA
    GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT
    TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC
    GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC
    CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001354400.1
    CDS317..3943
    Translation

    Target ORF information:

    RefSeq Version NM_001367471.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001367471.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG
    AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG
    GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC
    AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC
    CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC
    CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC
    ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC
    ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT
    ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA
    GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC
    CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA
    ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT
    CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG
    CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA
    GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG
    ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC
    ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC
    AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC
    TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA
    GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT
    TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC
    GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC
    CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09585
    Clone ID Related Accession (Same CDS sequence) XM_005253014.2 , NM_001367471.1 , XM_005253014.3 , NM_017749.3
    Accession Version NM_017749.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC045609.1, DB232750.1, AK023197.1 and BF589843.1. On Jun 20, 2012 this sequence version replaced NM_017749.2. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2 and 8 both encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC045609.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG
    AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG
    GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC
    AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC
    CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC
    CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC
    ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC
    ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT
    ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA
    GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC
    CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA
    ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT
    CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG
    CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA
    GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG
    ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC
    ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC
    AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC
    TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA
    GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT
    TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC
    GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC
    CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060219.2
    CDS361..3987
    Translation

    Target ORF information:

    RefSeq Version NM_017749.3
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_017749.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG
    AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG
    GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC
    AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC
    CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC
    CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC
    ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC
    ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT
    ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA
    GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC
    CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA
    ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT
    CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG
    CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA
    GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG
    ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC
    ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC
    AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC
    TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA
    GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT
    TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC
    GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC
    CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09779
    Clone ID Related Accession (Same CDS sequence) NM_001267783.1 , NM_001267783.2
    Accession Version NM_001267783.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB070055.1, AL834190.1, DQ870924.1 and BF589843.1. Transcript Variant: This variant (3) lacks two consecutive exons in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.69365.1, SRR1660805.26538.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC ACGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAACCGTC TCCGTTCTTC CACCTCCTCC
    TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT
    GAGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT
    TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC
    GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC
    TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT
    CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG
    CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC
    CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC
    AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG
    AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG
    TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC
    ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC
    AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT
    GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG
    CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT
    GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC
    CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG
    GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001254712.1
    CDS363..3902
    Translation

    Target ORF information:

    RefSeq Version NM_001267783.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001267783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC ACGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAACCGTC TCCGTTCTTC CACCTCCTCC
    TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT
    GAGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT
    TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC
    GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC
    TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT
    CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG
    CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC
    CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC
    AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG
    AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG
    TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC
    ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC
    AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT
    GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG
    CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT
    GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC
    CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG
    GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09779
    Clone ID Related Accession (Same CDS sequence) NM_001267783.1 , NM_001267783.2
    Accession Version NM_001267783.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB070055.1, AL834190.1 and DQ870924.1. On Jun 2, 2019 this sequence version replaced NM_001267783.1. Transcript Variant: This variant (3) lacks two consecutive exons in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL834190.1, SRR1660805.69365.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC ACGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAACCGTC TCCGTTCTTC CACCTCCTCC
    TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT
    GAGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT
    TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC
    GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC
    TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT
    CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG
    CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC
    CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC
    AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG
    AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG
    TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC
    ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC
    AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT
    GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG
    CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT
    GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC
    CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG
    GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001254712.1
    CDS317..3856
    Translation

    Target ORF information:

    RefSeq Version NM_001267783.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001267783.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC ACGGAGAGAC
    AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAACCGTC TCCGTTCTTC CACCTCCTCC
    TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT
    GAGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT
    TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC
    GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC
    TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT
    CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG
    CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC
    CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC
    AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG
    AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG
    TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC
    ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC
    AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT
    GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG
    CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT
    GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC
    CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG
    GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12050
    Clone ID Related Accession (Same CDS sequence) NM_001267782.1 , NM_001267782.2
    Accession Version NM_001267782.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB070055.1, AK000301.1, BC045609.1, AC115097.6 and BF589843.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK000301.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGACGGAGTC TTGCTCTGTC ACCTAGGCTG GAGTACAGCG GCGCTATCTT GGCTCACTGC
    AAGCTCCGCC TCCCAGGTTC ATGCCATTCT CCTGCCTCAG CTTCCCAAGT AGCTGGGACT
    ACGGGAGCCC ACCACCACGC CCGGCTAATT TTTGCATTTT TAGTAGAGAT GGAGTTTCAC
    CATGTTAGCC AGGCTGGTCT CGAACTCCTG ACCTCAGGTG ATCTGCCCAC CTCGGCCTCC
    CAAAGTGCTG GGATTACAGG CGTGAGCCAC CGCGCCTGGC CGAGGCTGCT GGAATCTTCC
    CTCATTTCAT TATCCCGTTA TGATGGAGCA GGATCCAGAG AGCACCCAAT TTACCCAGAC
    CCAGCGAGAT TATCTCCTGC TGCATACTAC GCCCAGAGGA TGATCCAGTA TCTCTCACGG
    AGAGACAGTA TTCGCCAGCG CTCCATGCGC TACCAACAGA ACCGTCTCCG TTCTTCCACC
    TCCTCCTCTT CCTCAGACAA CCAGGGTCCA TCAGTAGAGG GAACCGACTT GGAATTTGAG
    GACTTTGAGG ACAATGGTGA CAGATCCAGG CACCGAGCTC CACGCAATGC CCGGATGTCT
    GCACCTTCGC TTGGACGCTT TGTCCCAAGG CGTTTCTTGC TGCCTGAGTA CTTGCCTTAT
    GCTGGGATTT TTCATGAACG TGGACAGCCT GGCTTGGCTA CTCACTCTTC TGTTAACAGG
    GTCCTGGCAG GGGCAGTGAT CGGTGATGGA CAGTCTGCTG TGGCCAGTAA CATTGCCAAT
    ACTACCTACC GGCTCCAGTG GTGGGACTTC ACTAAGTTTG ACCTCCCTGA AATCAGTAAT
    GCTTCCGTGA ATGTGCTGGT GCAGAACTGC AAGATCTACA ATGATGCCAG CTGTGACATT
    TCTGCAGATG GCCAGCTCCT GGCAGCTTTC ATCCCCAGCA GCCAGAGGGG CTTTCCTGAT
    GAAGGCATCC TGGCAGTGTA CTCCCTGGCC CCCCATAACC TGGGCGAAAT GCTCTACACC
    AAGCGATTTG GTCCCAATGC CATTTCGGTG AGCCTGTCCC CAATGGGCAG ATATGTAATG
    GTGGGCTTGG CCTCACGAAG GATCCTGCTG CACCCCTCCA CAGAGCACAT GGTGGCCCAG
    GTCTTCAGGC TGCAACAGGC CCATGGTGGA GAGACCTCCA TGAGGAGAGT TTTCAACGTC
    CTTTATCCCA TGCCTGCCGA CCAGCGGAGA CATGTCAGTA TCAACTCTGC CCGTTGGCTG
    CCTGAGCCAG GGCTTGGCTT GGCCTATGGT ACTAACAAAG GAGACCTGGT GATCTGCCGA
    CCAGAGGCCT TAAACTCTGG TGTTGAGTAC TACTGGGACC AGCTGAACGA GACGGTCTTC
    ACTGTCCATT CCAACAGCAG GAGCAGCGAG CGGCCTGGAA CCAGCAGAGC CACATGGAGG
    ACAGACAGAG ACATGGGGCT GATGAATGCC ATTGGGCTTC AGCCCCGGAA CCCTGCCACC
    TCAGTGACAT CTCAGGGCAC CCAGACTCTG GCCCTTCAGC TGCAGAATGC CGAAACACAG
    ACTGAGAGGG AGGTGCCGGA GCCAGGGACA GCCGCCTCAG GTCCTGGTGA AGGTGAGGGT
    TCAGAGTATG GTGCCAGTGG AGAAGATGCG CTCAGCAGGA TCCAGAGGCT GATGGCGGAG
    GGCGGCATGA CAGCCGTGGT GCAGCGGGAG CAGAGCACCA CCATGGCCTC CATGGGCGGC
    TTCGGCAACA ACATCATCGT CAGCCACCGC ATTCACCGCA GCTCTCAGAC GGGCACTGAA
    CCTGGTGCCG CCCACACCTC CTCACCCCAG CCCTCCACCT CTCGGGGACT GCTCCCAGAG
    GCCGGGCAAC TGGCAGAGCG AGGCCTAAGC CCCCGGACAG CTTCCTGGGA CCAGCCTGGT
    ACCCCTGGGC GGGAGCCAAC CCAGCCAACC CTGCCCTCTT CCTCCCCTGT CCCCATTCCT
    GTTTCCCTTC CCAGCGCTGA GGGACCAACC CTCCACTGCG AGTTGACCAA TAACAACCAC
    CTTCTGGATG GTGGCAGCAG CAGGGGGGAC GCTGCAGGCC CTAGGGGAGA ACCACGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001254711.1
    CDS363..4268
    Translation

    Target ORF information:

    RefSeq Version NM_001267782.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001267782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGACGGAGTC TTGCTCTGTC ACCTAGGCTG GAGTACAGCG GCGCTATCTT GGCTCACTGC
    AAGCTCCGCC TCCCAGGTTC ATGCCATTCT CCTGCCTCAG CTTCCCAAGT AGCTGGGACT
    ACGGGAGCCC ACCACCACGC CCGGCTAATT TTTGCATTTT TAGTAGAGAT GGAGTTTCAC
    CATGTTAGCC AGGCTGGTCT CGAACTCCTG ACCTCAGGTG ATCTGCCCAC CTCGGCCTCC
    CAAAGTGCTG GGATTACAGG CGTGAGCCAC CGCGCCTGGC CGAGGCTGCT GGAATCTTCC
    CTCATTTCAT TATCCCGTTA TGATGGAGCA GGATCCAGAG AGCACCCAAT TTACCCAGAC
    CCAGCGAGAT TATCTCCTGC TGCATACTAC GCCCAGAGGA TGATCCAGTA TCTCTCACGG
    AGAGACAGTA TTCGCCAGCG CTCCATGCGC TACCAACAGA ACCGTCTCCG TTCTTCCACC
    TCCTCCTCTT CCTCAGACAA CCAGGGTCCA TCAGTAGAGG GAACCGACTT GGAATTTGAG
    GACTTTGAGG ACAATGGTGA CAGATCCAGG CACCGAGCTC CACGCAATGC CCGGATGTCT
    GCACCTTCGC TTGGACGCTT TGTCCCAAGG CGTTTCTTGC TGCCTGAGTA CTTGCCTTAT
    GCTGGGATTT TTCATGAACG TGGACAGCCT GGCTTGGCTA CTCACTCTTC TGTTAACAGG
    GTCCTGGCAG GGGCAGTGAT CGGTGATGGA CAGTCTGCTG TGGCCAGTAA CATTGCCAAT
    ACTACCTACC GGCTCCAGTG GTGGGACTTC ACTAAGTTTG ACCTCCCTGA AATCAGTAAT
    GCTTCCGTGA ATGTGCTGGT GCAGAACTGC AAGATCTACA ATGATGCCAG CTGTGACATT
    TCTGCAGATG GCCAGCTCCT GGCAGCTTTC ATCCCCAGCA GCCAGAGGGG CTTTCCTGAT
    GAAGGCATCC TGGCAGTGTA CTCCCTGGCC CCCCATAACC TGGGCGAAAT GCTCTACACC
    AAGCGATTTG GTCCCAATGC CATTTCGGTG AGCCTGTCCC CAATGGGCAG ATATGTAATG
    GTGGGCTTGG CCTCACGAAG GATCCTGCTG CACCCCTCCA CAGAGCACAT GGTGGCCCAG
    GTCTTCAGGC TGCAACAGGC CCATGGTGGA GAGACCTCCA TGAGGAGAGT TTTCAACGTC
    CTTTATCCCA TGCCTGCCGA CCAGCGGAGA CATGTCAGTA TCAACTCTGC CCGTTGGCTG
    CCTGAGCCAG GGCTTGGCTT GGCCTATGGT ACTAACAAAG GAGACCTGGT GATCTGCCGA
    CCAGAGGCCT TAAACTCTGG TGTTGAGTAC TACTGGGACC AGCTGAACGA GACGGTCTTC
    ACTGTCCATT CCAACAGCAG GAGCAGCGAG CGGCCTGGAA CCAGCAGAGC CACATGGAGG
    ACAGACAGAG ACATGGGGCT GATGAATGCC ATTGGGCTTC AGCCCCGGAA CCCTGCCACC
    TCAGTGACAT CTCAGGGCAC CCAGACTCTG GCCCTTCAGC TGCAGAATGC CGAAACACAG
    ACTGAGAGGG AGGTGCCGGA GCCAGGGACA GCCGCCTCAG GTCCTGGTGA AGGTGAGGGT
    TCAGAGTATG GTGCCAGTGG AGAAGATGCG CTCAGCAGGA TCCAGAGGCT GATGGCGGAG
    GGCGGCATGA CAGCCGTGGT GCAGCGGGAG CAGAGCACCA CCATGGCCTC CATGGGCGGC
    TTCGGCAACA ACATCATCGT CAGCCACCGC ATTCACCGCA GCTCTCAGAC GGGCACTGAA
    CCTGGTGCCG CCCACACCTC CTCACCCCAG CCCTCCACCT CTCGGGGACT GCTCCCAGAG
    GCCGGGCAAC TGGCAGAGCG AGGCCTAAGC CCCCGGACAG CTTCCTGGGA CCAGCCTGGT
    ACCCCTGGGC GGGAGCCAAC CCAGCCAACC CTGCCCTCTT CCTCCCCTGT CCCCATTCCT
    GTTTCCCTTC CCAGCGCTGA GGGACCAACC CTCCACTGCG AGTTGACCAA TAACAACCAC
    CTTCTGGATG GTGGCAGCAG CAGGGGGGAC GCTGCAGGCC CTAGGGGAGA ACCACGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12050
    Clone ID Related Accession (Same CDS sequence) NM_001267782.1 , NM_001267782.2
    Accession Version NM_001267782.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB070055.1, AK000301.1, BC045609.1 and AC115097.6. On Jun 3, 2019 this sequence version replaced NM_001267782.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK000301.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGACGGAGTC TTGCTCTGTC ACCTAGGCTG GAGTACAGCG GCGCTATCTT GGCTCACTGC
    AAGCTCCGCC TCCCAGGTTC ATGCCATTCT CCTGCCTCAG CTTCCCAAGT AGCTGGGACT
    ACGGGAGCCC ACCACCACGC CCGGCTAATT TTTGCATTTT TAGTAGAGAT GGAGTTTCAC
    CATGTTAGCC AGGCTGGTCT CGAACTCCTG ACCTCAGGTG ATCTGCCCAC CTCGGCCTCC
    CAAAGTGCTG GGATTACAGG CGTGAGCCAC CGCGCCTGGC CGAGGCTGCT GGAATCTTCC
    CTCATTTCAT TATCCCGTTA TGATGGAGCA GGATCCAGAG AGCACCCAAT TTACCCAGAC
    CCAGCGAGAT TATCTCCTGC TGCATACTAC GCCCAGAGGA TGATCCAGTA TCTCTCACGG
    AGAGACAGTA TTCGCCAGCG CTCCATGCGC TACCAACAGA ACCGTCTCCG TTCTTCCACC
    TCCTCCTCTT CCTCAGACAA CCAGGGTCCA TCAGTAGAGG GAACCGACTT GGAATTTGAG
    GACTTTGAGG ACAATGGTGA CAGATCCAGG CACCGAGCTC CACGCAATGC CCGGATGTCT
    GCACCTTCGC TTGGACGCTT TGTCCCAAGG CGTTTCTTGC TGCCTGAGTA CTTGCCTTAT
    GCTGGGATTT TTCATGAACG TGGACAGCCT GGCTTGGCTA CTCACTCTTC TGTTAACAGG
    GTCCTGGCAG GGGCAGTGAT CGGTGATGGA CAGTCTGCTG TGGCCAGTAA CATTGCCAAT
    ACTACCTACC GGCTCCAGTG GTGGGACTTC ACTAAGTTTG ACCTCCCTGA AATCAGTAAT
    GCTTCCGTGA ATGTGCTGGT GCAGAACTGC AAGATCTACA ATGATGCCAG CTGTGACATT
    TCTGCAGATG GCCAGCTCCT GGCAGCTTTC ATCCCCAGCA GCCAGAGGGG CTTTCCTGAT
    GAAGGCATCC TGGCAGTGTA CTCCCTGGCC CCCCATAACC TGGGCGAAAT GCTCTACACC
    AAGCGATTTG GTCCCAATGC CATTTCGGTG AGCCTGTCCC CAATGGGCAG ATATGTAATG
    GTGGGCTTGG CCTCACGAAG GATCCTGCTG CACCCCTCCA CAGAGCACAT GGTGGCCCAG
    GTCTTCAGGC TGCAACAGGC CCATGGTGGA GAGACCTCCA TGAGGAGAGT TTTCAACGTC
    CTTTATCCCA TGCCTGCCGA CCAGCGGAGA CATGTCAGTA TCAACTCTGC CCGTTGGCTG
    CCTGAGCCAG GGCTTGGCTT GGCCTATGGT ACTAACAAAG GAGACCTGGT GATCTGCCGA
    CCAGAGGCCT TAAACTCTGG TGTTGAGTAC TACTGGGACC AGCTGAACGA GACGGTCTTC
    ACTGTCCATT CCAACAGCAG GAGCAGCGAG CGGCCTGGAA CCAGCAGAGC CACATGGAGG
    ACAGACAGAG ACATGGGGCT GATGAATGCC ATTGGGCTTC AGCCCCGGAA CCCTGCCACC
    TCAGTGACAT CTCAGGGCAC CCAGACTCTG GCCCTTCAGC TGCAGAATGC CGAAACACAG
    ACTGAGAGGG AGGTGCCGGA GCCAGGGACA GCCGCCTCAG GTCCTGGTGA AGGTGAGGGT
    TCAGAGTATG GTGCCAGTGG AGAAGATGCG CTCAGCAGGA TCCAGAGGCT GATGGCGGAG
    GGCGGCATGA CAGCCGTGGT GCAGCGGGAG CAGAGCACCA CCATGGCCTC CATGGGCGGC
    TTCGGCAACA ACATCATCGT CAGCCACCGC ATTCACCGCA GCTCTCAGAC GGGCACTGAA
    CCTGGTGCCG CCCACACCTC CTCACCCCAG CCCTCCACCT CTCGGGGACT GCTCCCAGAG
    GCCGGGCAAC TGGCAGAGCG AGGCCTAAGC CCCCGGACAG CTTCCTGGGA CCAGCCTGGT
    ACCCCTGGGC GGGAGCCAAC CCAGCCAACC CTGCCCTCTT CCTCCCCTGT CCCCATTCCT
    GTTTCCCTTC CCAGCGCTGA GGGACCAACC CTCCACTGCG AGTTGACCAA TAACAACCAC
    CTTCTGGATG GTGGCAGCAG CAGGGGGGAC GCTGCAGGCC CTAGGGGAGA ACCACGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001254711.1
    CDS317..4222
    Translation

    Target ORF information:

    RefSeq Version NM_001267782.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001267782.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGACGGAGTC TTGCTCTGTC ACCTAGGCTG GAGTACAGCG GCGCTATCTT GGCTCACTGC
    AAGCTCCGCC TCCCAGGTTC ATGCCATTCT CCTGCCTCAG CTTCCCAAGT AGCTGGGACT
    ACGGGAGCCC ACCACCACGC CCGGCTAATT TTTGCATTTT TAGTAGAGAT GGAGTTTCAC
    CATGTTAGCC AGGCTGGTCT CGAACTCCTG ACCTCAGGTG ATCTGCCCAC CTCGGCCTCC
    CAAAGTGCTG GGATTACAGG CGTGAGCCAC CGCGCCTGGC CGAGGCTGCT GGAATCTTCC
    CTCATTTCAT TATCCCGTTA TGATGGAGCA GGATCCAGAG AGCACCCAAT TTACCCAGAC
    CCAGCGAGAT TATCTCCTGC TGCATACTAC GCCCAGAGGA TGATCCAGTA TCTCTCACGG
    AGAGACAGTA TTCGCCAGCG CTCCATGCGC TACCAACAGA ACCGTCTCCG TTCTTCCACC
    TCCTCCTCTT CCTCAGACAA CCAGGGTCCA TCAGTAGAGG GAACCGACTT GGAATTTGAG
    GACTTTGAGG ACAATGGTGA CAGATCCAGG CACCGAGCTC CACGCAATGC CCGGATGTCT
    GCACCTTCGC TTGGACGCTT TGTCCCAAGG CGTTTCTTGC TGCCTGAGTA CTTGCCTTAT
    GCTGGGATTT TTCATGAACG TGGACAGCCT GGCTTGGCTA CTCACTCTTC TGTTAACAGG
    GTCCTGGCAG GGGCAGTGAT CGGTGATGGA CAGTCTGCTG TGGCCAGTAA CATTGCCAAT
    ACTACCTACC GGCTCCAGTG GTGGGACTTC ACTAAGTTTG ACCTCCCTGA AATCAGTAAT
    GCTTCCGTGA ATGTGCTGGT GCAGAACTGC AAGATCTACA ATGATGCCAG CTGTGACATT
    TCTGCAGATG GCCAGCTCCT GGCAGCTTTC ATCCCCAGCA GCCAGAGGGG CTTTCCTGAT
    GAAGGCATCC TGGCAGTGTA CTCCCTGGCC CCCCATAACC TGGGCGAAAT GCTCTACACC
    AAGCGATTTG GTCCCAATGC CATTTCGGTG AGCCTGTCCC CAATGGGCAG ATATGTAATG
    GTGGGCTTGG CCTCACGAAG GATCCTGCTG CACCCCTCCA CAGAGCACAT GGTGGCCCAG
    GTCTTCAGGC TGCAACAGGC CCATGGTGGA GAGACCTCCA TGAGGAGAGT TTTCAACGTC
    CTTTATCCCA TGCCTGCCGA CCAGCGGAGA CATGTCAGTA TCAACTCTGC CCGTTGGCTG
    CCTGAGCCAG GGCTTGGCTT GGCCTATGGT ACTAACAAAG GAGACCTGGT GATCTGCCGA
    CCAGAGGCCT TAAACTCTGG TGTTGAGTAC TACTGGGACC AGCTGAACGA GACGGTCTTC
    ACTGTCCATT CCAACAGCAG GAGCAGCGAG CGGCCTGGAA CCAGCAGAGC CACATGGAGG
    ACAGACAGAG ACATGGGGCT GATGAATGCC ATTGGGCTTC AGCCCCGGAA CCCTGCCACC
    TCAGTGACAT CTCAGGGCAC CCAGACTCTG GCCCTTCAGC TGCAGAATGC CGAAACACAG
    ACTGAGAGGG AGGTGCCGGA GCCAGGGACA GCCGCCTCAG GTCCTGGTGA AGGTGAGGGT
    TCAGAGTATG GTGCCAGTGG AGAAGATGCG CTCAGCAGGA TCCAGAGGCT GATGGCGGAG
    GGCGGCATGA CAGCCGTGGT GCAGCGGGAG CAGAGCACCA CCATGGCCTC CATGGGCGGC
    TTCGGCAACA ACATCATCGT CAGCCACCGC ATTCACCGCA GCTCTCAGAC GGGCACTGAA
    CCTGGTGCCG CCCACACCTC CTCACCCCAG CCCTCCACCT CTCGGGGACT GCTCCCAGAG
    GCCGGGCAAC TGGCAGAGCG AGGCCTAAGC CCCCGGACAG CTTCCTGGGA CCAGCCTGGT
    ACCCCTGGGC GGGAGCCAAC CCAGCCAACC CTGCCCTCTT CCTCCCCTGT CCCCATTCCT
    GTTTCCCTTC CCAGCGCTGA GGGACCAACC CTCCACTGCG AGTTGACCAA TAACAACCAC
    CTTCTGGATG GTGGCAGCAG CAGGGGGGAC GCTGCAGGCC CTAGGGGAGA ACCACGGAAC
    AGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44914
    Clone ID Related Accession (Same CDS sequence) NM_001367468.1 , XM_005253009.4 , XM_024448605.1 , XM_005253009.3 , XM_006718259.2
    Accession Version NM_001367468.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform 5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK302808.1, AC127035.6 and AC116021.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302808.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT
    TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001354397.1
    CDS361..4257
    Translation

    Target ORF information:

    RefSeq Version NM_001367468.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001367468.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT
    TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44916
    Clone ID Related Accession (Same CDS sequence) NM_001300731.1 , NM_001300731.2
    Accession Version NM_001300731.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB070055.1, AB051523.1 and BF589843.1. On Jul 11, 2014 this sequence version replaced XM_005253012.2. Transcript Variant: This variant (4) retains an alternate in-frame intron and lacks two alternate in-frame exons compared to variant 1. The resulting isoform (4) contains an alternate internal segment and lacks two other alternate internal segments compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB051523.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287660.1
    CDS363..4079
    Translation

    Target ORF information:

    RefSeq Version NM_001300731.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300731.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44916
    Clone ID Related Accession (Same CDS sequence) NM_001300731.1 , NM_001300731.2
    Accession Version NM_001300731.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB070055.1 and AB051523.1. On Jun 2, 2019 this sequence version replaced NM_001300731.1. Transcript Variant: This variant (4) retains an alternate in-frame intron and lacks two alternate in-frame exons compared to variant 1. The resulting isoform (4) contains an alternate internal segment and lacks two other alternate internal segments compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB051523.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287660.1
    CDS317..4033
    Translation

    Target ORF information:

    RefSeq Version NM_001300731.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300731.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44917
    Clone ID Related Accession (Same CDS sequence) XM_006718260.2 , NM_001367469.1
    Accession Version NM_001367469.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform 6
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127035.6, AC115097.6, AC024293.18 and AC116021.5. On Dec 1, 2018 this sequence version replaced XM_006718260.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.151930.1, SRR1660809.19725.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT
    TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC
    GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC
    TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT
    CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG
    CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC
    CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC
    AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG
    AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG
    TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC
    ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC
    AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT
    GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG
    CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT
    GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC
    CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG
    GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001354398.1
    CDS317..3676
    Translation

    Target ORF information:

    RefSeq Version NM_001367469.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001367469.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT
    TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC
    GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC
    TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT
    CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG
    CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC
    CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC
    AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG
    AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG
    TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC
    ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC
    AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT
    GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG
    CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT
    GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC
    CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG
    GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09585
    Clone ID Related Accession (Same CDS sequence) XM_005253014.2 , NM_001367471.1 , XM_005253014.3 , NM_017749.3
    Accession Version XM_005253014.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG
    AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG
    GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC
    AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC
    CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC
    CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC
    ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC
    ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT
    ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA
    GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC
    CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA
    ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT
    CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG
    CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA
    GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG
    ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC
    ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC
    AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC
    TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA
    GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT
    TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC
    GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC
    CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005253071.1
    Translation

    Target ORF information:

    RefSeq Version XM_005253014.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005253014.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG
    AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG
    GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC
    AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC
    CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC
    CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC
    ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC
    ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT
    ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA
    GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC
    CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA
    ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT
    CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG
    CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA
    GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG
    ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC
    ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC
    AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC
    TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA
    GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT
    TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC
    GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC
    CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09585
    Clone ID Related Accession (Same CDS sequence) XM_005253014.2 , NM_001367471.1 , XM_005253014.3 , NM_017749.3
    Accession Version XM_005253014.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253014.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG
    AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG
    GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC
    AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC
    CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC
    CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC
    ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC
    ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT
    ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA
    GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC
    CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA
    ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT
    CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG
    CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA
    GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG
    ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC
    ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC
    AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC
    TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA
    GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT
    TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC
    GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC
    CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005253071.1
    CDS359..3985
    Translation

    Target ORF information:

    RefSeq Version XM_005253014.3
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005253014.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA
    GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG
    ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG
    AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG
    GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT
    CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG
    CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT
    ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT
    GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT
    GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC
    AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC
    AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC
    CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC
    CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC
    ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC
    ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT
    ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA
    GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC
    CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA
    ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT
    CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG
    CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA
    GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG
    ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC
    ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC
    AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC
    TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA
    GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT
    TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC
    GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC
    CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44914
    Clone ID Related Accession (Same CDS sequence) NM_001367468.1 , XM_005253009.4 , XM_024448605.1 , XM_005253009.3 , XM_006718259.2
    Accession Version XM_005253009.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005253009.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT
    TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005253066.1
    CDS349..4245
    Translation

    Target ORF information:

    RefSeq Version XM_005253009.4
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005253009.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT
    TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44914
    Clone ID Related Accession (Same CDS sequence) NM_001367468.1 , XM_005253009.4 , XM_024448605.1 , XM_005253009.3 , XM_006718259.2
    Accession Version XM_024448605.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT
    TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024304373.1
    CDS713..4609
    Translation

    Target ORF information:

    RefSeq Version XM_024448605.1
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024448605.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT
    TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44914
    Clone ID Related Accession (Same CDS sequence) NM_001367468.1 , XM_005253009.4 , XM_024448605.1 , XM_005253009.3 , XM_006718259.2
    Accession Version XM_005253009.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT
    TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005253066.1
    CDS359..4255
    Translation

    Target ORF information:

    RefSeq Version XM_005253009.3
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005253009.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT
    TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44914
    Clone ID Related Accession (Same CDS sequence) NM_001367468.1 , XM_005253009.4 , XM_024448605.1 , XM_005253009.3 , XM_006718259.2
    Accession Version XM_006718259.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT
    TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718322.1
    CDS240..4136
    Translation

    Target ORF information:

    RefSeq Version XM_006718259.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718259.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA
    TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA
    TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT
    ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT
    TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG
    GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG
    CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT
    TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA
    GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC
    CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG
    AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT
    GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC
    CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT
    GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG
    GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG
    CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC
    ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA
    GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC
    TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT
    TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA
    GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA
    TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG
    GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT
    GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG
    ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC
    AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC
    GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA
    CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG
    CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT
    CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT
    GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44915
    Clone ID Related Accession (Same CDS sequence) XM_005253011.4 , XM_005253011.3
    Accession Version XM_005253011.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005253011.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGATTATCTC CTGCTGCATA CTACGCCCAG
    AGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTACCAA
    CAGAACCGTC TCCGTTCTTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCAGTA
    GAGGGAACCG ACTTGGAATT TGAGGACTTT GAGGACAATG GTGACAGATC CAGGCACCGA
    GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC
    TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG
    GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT
    GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG
    TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC
    TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC
    AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT
    AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG
    TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC
    TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC
    TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC
    AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC
    AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG
    GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT
    GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG
    CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT
    CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC
    TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC
    AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC
    ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC
    CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC
    ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG
    ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC
    TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC
    TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA
    GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005253068.1
    CDS349..4158
    Translation

    Target ORF information:

    RefSeq Version XM_005253011.4
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005253011.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGATTATCTC CTGCTGCATA CTACGCCCAG
    AGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTACCAA
    CAGAACCGTC TCCGTTCTTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCAGTA
    GAGGGAACCG ACTTGGAATT TGAGGACTTT GAGGACAATG GTGACAGATC CAGGCACCGA
    GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC
    TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG
    GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT
    GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG
    TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC
    TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC
    AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT
    AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG
    TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC
    TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC
    TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC
    AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC
    AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG
    GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT
    GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG
    CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT
    CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC
    TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC
    AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC
    ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC
    CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC
    ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG
    ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC
    TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC
    TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA
    GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44915
    Clone ID Related Accession (Same CDS sequence) XM_005253011.4 , XM_005253011.3
    Accession Version XM_005253011.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253011.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGATTATCTC CTGCTGCATA CTACGCCCAG
    AGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTACCAA
    CAGAACCGTC TCCGTTCTTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCAGTA
    GAGGGAACCG ACTTGGAATT TGAGGACTTT GAGGACAATG GTGACAGATC CAGGCACCGA
    GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC
    TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG
    GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT
    GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG
    TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC
    TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC
    AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT
    AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG
    TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC
    TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC
    TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC
    AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC
    AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG
    GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT
    GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG
    CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT
    CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC
    TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC
    AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC
    ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC
    CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC
    ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG
    ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC
    TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC
    TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA
    GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005253068.1
    CDS359..4168
    Translation

    Target ORF information:

    RefSeq Version XM_005253011.3
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005253011.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC
    ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG
    CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG
    TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT
    TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC
    TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG
    GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC
    AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT
    AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC
    CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC
    AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA
    TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG
    TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG
    TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG
    AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT
    GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG
    TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT
    ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG
    CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG
    CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG
    CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT
    TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT
    GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGATTATCTC CTGCTGCATA CTACGCCCAG
    AGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTACCAA
    CAGAACCGTC TCCGTTCTTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCAGTA
    GAGGGAACCG ACTTGGAATT TGAGGACTTT GAGGACAATG GTGACAGATC CAGGCACCGA
    GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC
    TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG
    GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT
    GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG
    TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC
    TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC
    AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT
    AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG
    TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC
    TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC
    TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC
    AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC
    AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG
    GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT
    GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG
    CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT
    CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC
    TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC
    AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC
    ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC
    CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC
    ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG
    ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC
    TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC
    TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA
    GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu44917
    Clone ID Related Accession (Same CDS sequence) XM_006718260.2 , NM_001367469.1
    Accession Version XM_006718260.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3360bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product activating molecule in BECN1-regulated autophagy protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718260.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT
    TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC
    GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC
    TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT
    CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG
    CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC
    CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC
    AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG
    AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG
    TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC
    ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC
    AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT
    GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG
    CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT
    GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC
    CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG
    GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718323.1
    CDS359..3718
    Translation

    Target ORF information:

    RefSeq Version XM_006718260.2
    Organism Homo sapiens(human)
    Definition Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718260.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
    CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG
    AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC
    TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG
    GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT
    GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT
    AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT
    GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC
    CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG
    GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT
    AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA
    TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC
    CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT
    CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC
    TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT
    ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA
    GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT
    GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG
    CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG
    GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC
    AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT
    GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC
    CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC
    CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT
    TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC
    TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA
    TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC
    AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT
    AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG
    TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG
    AGGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT
    TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG
    ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG
    GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC
    TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC
    GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA
    GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC
    ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA
    TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC
    TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC
    AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT
    CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG
    CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG
    GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC
    CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC
    AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG
    ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG
    AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG
    TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC
    ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC
    AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT
    GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG
    CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT
    GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC
    CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG
    GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.