LGR4 cDNA ORF clone, Homo sapiens(human)

The following LGR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LGR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu27175 NM_018490.4
Latest version!
Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu109417 NM_018490.5
Latest version!
Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu100713 NM_001346432.1
Latest version!
Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
View Old Accession Versions >>
OHu27175 NM_018490.2 Homo sapiens leucine-rich repeat containing G protein-coupled receptor 4 (LGR4), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu27175 NM_018490.3 Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu27175
    Clone ID Related Accession (Same CDS sequence) NM_018490.3 , NM_018490.2 , NM_018490.4
    Accession Version NM_018490.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-11-09
    Organism Homo sapiens(human)
    Product leucine-rich repeat-containing G-protein coupled receptor 4 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC033039.2, DR000664.1, AB209743.1, AF257182.1 and AC090597.9. This sequence is a reference standard in the RefSeqGene project. On Oct 12, 2016 this sequence version replaced NM_018490.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF257182.1, SRR1803614.192490.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
    CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG
    GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA
    GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT
    CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC
    TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA
    CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT
    ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG
    GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG
    GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT
    TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT
    TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT
    CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT
    TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT
    GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC
    CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC
    CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA
    GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT
    TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA
    AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG
    ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA
    AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA
    CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG
    TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA
    AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT
    GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC
    CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG
    ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT
    TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT
    TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG
    TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA
    GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT
    GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA
    CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC
    CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA
    ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA
    ATGGCCGTTA TCTACACTAA GCTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC
    TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC
    TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA
    ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG
    TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC
    AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT
    TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC
    TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC
    TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC
    ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT
    GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG
    CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060960.2
    CDS474..3329
    Translation

    Target ORF information:

    RefSeq Version NM_018490.3
    Organism Homo sapiens(human)
    Definition Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018490.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
    CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG
    GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA
    GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT
    CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC
    TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA
    CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT
    ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG
    GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG
    GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT
    TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT
    TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT
    CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT
    TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT
    GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC
    CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC
    CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA
    GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT
    TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA
    AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG
    ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA
    AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA
    CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG
    TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA
    AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT
    GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC
    CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG
    ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT
    TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT
    TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG
    TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA
    GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT
    GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA
    CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC
    CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA
    ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA
    ATGGCCGTTA TCTACACTAA GCTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC
    TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC
    TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA
    ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG
    TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC
    AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT
    TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC
    TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC
    TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC
    ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT
    GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG
    CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27175
    Clone ID Related Accession (Same CDS sequence) NM_018490.3 , NM_018490.2 , NM_018490.4
    Accession Version NM_018490.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product leucine-rich repeat-containing G-protein coupled receptor 4 precursor
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
    CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG
    GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA
    GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT
    CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC
    TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA
    CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT
    ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG
    GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG
    GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT
    TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT
    TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT
    CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT
    TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT
    GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC
    CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC
    CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA
    GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT
    TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA
    AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG
    ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA
    AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA
    CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG
    TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA
    AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT
    GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC
    CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG
    ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT
    TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT
    TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG
    TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA
    GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT
    GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA
    CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC
    CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA
    ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA
    ATGGCCGTTA TCTACACTAA GCTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC
    TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC
    TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA
    ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG
    TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC
    AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT
    TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC
    TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC
    TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC
    ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT
    GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG
    CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060960.2
    Translation

    Target ORF information:

    RefSeq Version NM_018490.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens leucine-rich repeat containing G protein-coupled receptor 4 (LGR4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018490.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
    CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG
    GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA
    GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT
    CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC
    TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA
    CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT
    ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG
    GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG
    GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT
    TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT
    TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT
    CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT
    TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT
    GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC
    CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC
    CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA
    GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT
    TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA
    AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG
    ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA
    AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA
    CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG
    TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA
    AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT
    GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC
    CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG
    ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT
    TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT
    TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG
    TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA
    GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT
    GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA
    CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC
    CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA
    ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA
    ATGGCCGTTA TCTACACTAA GCTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC
    TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC
    TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA
    ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG
    TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC
    AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT
    TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC
    TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC
    TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC
    ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT
    GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG
    CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27175
    Clone ID Related Accession (Same CDS sequence) NM_018490.3 , NM_018490.2 , NM_018490.4
    Accession Version NM_018490.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-20
    Organism Homo sapiens(human)
    Product leucine-rich repeat-containing G-protein coupled receptor 4 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC100771.2, BC033039.2, DR000664.1, AB209743.1, AF257182.1 and AC090597.9. On Nov 24, 2018 this sequence version replaced NM_018490.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF257182.1, SRR1803614.192490.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
    CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG
    GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA
    GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT
    CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC
    TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA
    CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT
    ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG
    GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG
    GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT
    TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT
    TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT
    CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT
    TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT
    GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC
    CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC
    CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA
    GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT
    TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA
    AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG
    ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA
    AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA
    CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG
    TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA
    AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT
    GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC
    CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG
    ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT
    TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT
    TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG
    TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA
    GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT
    GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA
    CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC
    CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA
    ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA
    ATGGCCGTTA TCTACACTAA GCTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC
    TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC
    TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA
    ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG
    TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC
    AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT
    TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC
    TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC
    TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC
    ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT
    GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG
    CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060960.2
    CDS489..3344
    Translation

    Target ORF information:

    RefSeq Version NM_018490.4
    Organism Homo sapiens(human)
    Definition Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018490.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
    CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG
    GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA
    GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT
    CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC
    TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA
    CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT
    ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG
    GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG
    GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT
    TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT
    TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT
    CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT
    TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT
    GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC
    CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC
    CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA
    GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT
    TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA
    AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG
    ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA
    AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA
    CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG
    TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA
    AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT
    GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC
    CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG
    ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT
    TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT
    TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG
    TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA
    GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT
    GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA
    CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC
    CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA
    ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA
    ATGGCCGTTA TCTACACTAA GCTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC
    TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC
    TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA
    ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG
    TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC
    AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT
    TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC
    TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC
    TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC
    ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT
    GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG
    CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109417
    Clone ID Related Accession (Same CDS sequence) NM_018490.5
    Accession Version NM_018490.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product leucine-rich repeat-containing G-protein coupled receptor 4 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC100771.2 and AC090597.9. On Aug 31, 2019 this sequence version replaced NM_018490.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF257182.1, AB209743.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000379214.9/ ENSP00000368516.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
    CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG
    GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA
    GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT
    CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC
    TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA
    CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT
    ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG
    GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG
    GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT
    TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT
    TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT
    CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT
    TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT
    GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC
    CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC
    CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA
    GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT
    TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA
    AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG
    ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA
    AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA
    CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG
    TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA
    AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT
    GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC
    CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG
    ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT
    TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT
    TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG
    TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA
    GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT
    GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA
    CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC
    CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA
    ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA
    ATGGCCGTTA TCTACACTAA ACTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC
    TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC
    TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA
    ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG
    TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC
    AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT
    TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC
    TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC
    TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC
    ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT
    GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG
    CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060960.2
    CDS489..3344
    Translation

    Target ORF information:

    RefSeq Version NM_018490.5
    Organism Homo sapiens(human)
    Definition Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018490.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
    CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG
    GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA
    GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT
    CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC
    TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA
    CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT
    ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG
    GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG
    GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT
    TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT
    TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT
    CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT
    TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT
    GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC
    CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC
    CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA
    GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT
    TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA
    AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG
    ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA
    AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA
    CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG
    TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA
    AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT
    GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC
    CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG
    ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT
    TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT
    TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG
    TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA
    GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT
    GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA
    CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC
    CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA
    ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA
    ATGGCCGTTA TCTACACTAA ACTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC
    TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC
    TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA
    ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG
    TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC
    AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT
    TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC
    TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC
    TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC
    ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT
    GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG
    CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100713
    Clone ID Related Accession (Same CDS sequence) NM_001346432.1
    Accession Version NM_001346432.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2784bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product leucine-rich repeat-containing G-protein coupled receptor 4 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC033039.2, DR000664.1, AB209743.1, AF257182.1 and AC090597.9. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033039.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
    CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG
    GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA
    GCGCTACAAT TGGCGGGCAA CGACCTTTCT TTTATCCACC CAAAGGCCTT GTCTGGGTTG
    AAAGAACTCA AAGTTCTAAC GCTCCAGAAT AATCAGTTGA AAACAGTACC CAGTGAAGCC
    ATTCGAGGGC TGAGTGCTTT GCAGTCTTTG CGTTTAGATG CCAACCATAT TACCTCAGTC
    CCCGAGGACA GTTTTGAAGG ACTTGTTCAG TTACGGCATC TGTGGCTGGA TGACAACAGC
    TTGACGGAGG TGCCTGTGCA CCCCCTCAGC AATCTGCCCA CCCTACAGGC GCTGACCCTG
    GCTCTCAACA AGATCTCAAG CATCCCTGAC TTTGCATTTA CCAACCTTTC AAGCCTGGTA
    GTTCTGCATC TTCATAACAA TAAAATTAGA AGCCTGAGTC AACACTGTTT TGATGGACTA
    GATAACCTGG AGACCTTAGA CTTGAATTAT AATAACTTGG GGGAATTTCC TCAGGCTATT
    AAAGCCCTTC CTAGCCTTAA AGAGCTAGGA TTTCATAGTA ATTCTATTTC TGTTATCCCT
    GATGGAGCAT TTGATGGTAA TCCACTCTTA AGAACTATAC ATTTGTATGA TAATCCTCTG
    TCTTTTGTGG GGAACTCAGC ATTTCACAAT TTATCTGATC TTCATTCCCT AGTCATTCGT
    GGTGCAAGCA TGGTGCAGCA GTTCCCCAAT CTTACAGGAA CTGTCCACCT GGAAAGTCTG
    ACTTTGACAG GTACAAAGAT AAGCAGCATA CCTAATAATT TGTGTCAAGA ACAAAAGATG
    CTTAGGACTT TGGACTTGTC TTACAATAAT ATAAGAGACC TTCCAAGTTT TAATGGTTGC
    CATGCTCTGG AAGAAATTTC TTTACAGCGT AATCAAATCT ACCAAATAAA GGAAGGCACC
    TTTCAAGGCC TGATATCTCT AAGGATTCTA GATCTGAGTA GAAACCTGAT ACATGAAATT
    CACAGTAGAG CTTTTGCCAC ACTTGGGCCA ATAACTAACC TAGATGTAAG TTTCAATGAA
    TTAACTTCCT TTCCTACGGA AGGCCTGAAT GGGCTAAATC AACTGAAACT TGTGGGCAAC
    TTCAAGCTGA AAGAAGCCTT AGCAGCAAAA GACTTTGTTA ACCTCAGGTC TTTATCAGTA
    CCATATGCTT ATCAGTGCTG TGCATTTTGG GGTTGTGACT CTTATGCAAA TTTAAACACA
    GAAGATAACA GCCTCCAGGA CCACAGTGTG GCACAGGAGA AAGGTACTGC TGATGCAGCA
    AATGTCACAA GCACTCTTGA AAATGAAGAA CATAGTCAAA TAATTATCCA TTGTACACCT
    TCAACAGGTG CTTTTAAGCC CTGTGAATAT TTACTGGGAA GCTGGATGAT TCGTCTTACT
    GTGTGGTTCA TTTTCTTGGT TGCATTATTT TTCAACCTGC TTGTTATTTT AACAACATTT
    GCATCTTGTA CATCACTGCC TTCGTCCAAA TTGTTTATAG GCTTGATTTC TGTGTCTAAC
    TTATTCATGG GAATCTATAC TGGCATCCTA ACTTTTCTTG ATGCTGTGTC CTGGGGCAGA
    TTCGCTGAAT TTGGCATTTG GTGGGAAACT GGCAGTGGCT GCAAAGTAGC TGGGTTTCTT
    GCAGTTTTCT CCTCAGAAAG TGCCATATTT TTATTAATGC TAGCAACTGT CGAAAGAAGC
    TTATCTGCAA AAGATATAAT GAAAAATGGG AAGAGCAATC ATCTCAAACA GTTCCGGGTT
    GCTGCCCTTT TGGCTTTCCT AGGTGCTACA GTAGCAGGCT GTTTTCCCCT TTTCCATAGA
    GGGGAATATT CTGCATCACC CCTTTGTTTG CCATTTCCTA CAGGTGAAAC GCCATCATTA
    GGATTCACTG TAACGTTAGT GCTATTAAAC TCACTAGCAT TTTTATTAAT GGCCGTTATC
    TACACTAAGC TATACTGCAA CTTGGAAAAA GAGGACCTCT CAGAAAACTC ACAATCTAGC
    ATGATTAAGC ATGTCGCTTG GCTAATCTTC ACCAATTGCA TCTTTTTCTG CCCTGTGGCG
    TTTTTTTCAT TTGCACCATT GATCACTGCA ATCTCTATCA GCCCCGAAAT AATGAAGTCT
    GTTACTCTGA TATTTTTTCC ATTGCCTGCT TGCCTGAATC CAGTCCTGTA TGTTTTCTTC
    AACCCAAAGT TTAAAGAAGA CTGGAAGTTA CTGAAGCGAC GTGTTACCAA GAAAAGTGGA
    TCAGTTTCAG TTTCCATCAG TAGCCAAGGT GGTTGTCTGG AACAGGATTT CTACTACGAC
    TGTGGCATGT ACTCACATTT GCAGGGCAAC CTGACTGTTT GCGACTGCTG CGAATCGTTT
    CTTTTAACAA AGCCAGTATC ATGCAAACAC TTGATAAAAT CACACAGCTG TCCTGCATTG
    GCAGTGGCTT CTTGCCAAAG ACCTGAGGGC TACTGGTCCG ACTGTGGCAC ACAGTCGGCC
    CACTCTGATT ATGCAGATGA AGAAGATTCC TTTGTCTCAG ACAGTTCTGA CCAGGTGCAG
    GCCTGTGGAC GAGCCTGCTT CTACCAGAGT AGAGGATTCC CTTTGGTGCG CTATGCTTAC
    AATCTACCAA GAGTTAAAGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333361.1
    CDS474..3257
    Translation

    Target ORF information:

    RefSeq Version NM_001346432.1
    Organism Homo sapiens(human)
    Definition Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346432.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
    CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG
    GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA
    GCGCTACAAT TGGCGGGCAA CGACCTTTCT TTTATCCACC CAAAGGCCTT GTCTGGGTTG
    AAAGAACTCA AAGTTCTAAC GCTCCAGAAT AATCAGTTGA AAACAGTACC CAGTGAAGCC
    ATTCGAGGGC TGAGTGCTTT GCAGTCTTTG CGTTTAGATG CCAACCATAT TACCTCAGTC
    CCCGAGGACA GTTTTGAAGG ACTTGTTCAG TTACGGCATC TGTGGCTGGA TGACAACAGC
    TTGACGGAGG TGCCTGTGCA CCCCCTCAGC AATCTGCCCA CCCTACAGGC GCTGACCCTG
    GCTCTCAACA AGATCTCAAG CATCCCTGAC TTTGCATTTA CCAACCTTTC AAGCCTGGTA
    GTTCTGCATC TTCATAACAA TAAAATTAGA AGCCTGAGTC AACACTGTTT TGATGGACTA
    GATAACCTGG AGACCTTAGA CTTGAATTAT AATAACTTGG GGGAATTTCC TCAGGCTATT
    AAAGCCCTTC CTAGCCTTAA AGAGCTAGGA TTTCATAGTA ATTCTATTTC TGTTATCCCT
    GATGGAGCAT TTGATGGTAA TCCACTCTTA AGAACTATAC ATTTGTATGA TAATCCTCTG
    TCTTTTGTGG GGAACTCAGC ATTTCACAAT TTATCTGATC TTCATTCCCT AGTCATTCGT
    GGTGCAAGCA TGGTGCAGCA GTTCCCCAAT CTTACAGGAA CTGTCCACCT GGAAAGTCTG
    ACTTTGACAG GTACAAAGAT AAGCAGCATA CCTAATAATT TGTGTCAAGA ACAAAAGATG
    CTTAGGACTT TGGACTTGTC TTACAATAAT ATAAGAGACC TTCCAAGTTT TAATGGTTGC
    CATGCTCTGG AAGAAATTTC TTTACAGCGT AATCAAATCT ACCAAATAAA GGAAGGCACC
    TTTCAAGGCC TGATATCTCT AAGGATTCTA GATCTGAGTA GAAACCTGAT ACATGAAATT
    CACAGTAGAG CTTTTGCCAC ACTTGGGCCA ATAACTAACC TAGATGTAAG TTTCAATGAA
    TTAACTTCCT TTCCTACGGA AGGCCTGAAT GGGCTAAATC AACTGAAACT TGTGGGCAAC
    TTCAAGCTGA AAGAAGCCTT AGCAGCAAAA GACTTTGTTA ACCTCAGGTC TTTATCAGTA
    CCATATGCTT ATCAGTGCTG TGCATTTTGG GGTTGTGACT CTTATGCAAA TTTAAACACA
    GAAGATAACA GCCTCCAGGA CCACAGTGTG GCACAGGAGA AAGGTACTGC TGATGCAGCA
    AATGTCACAA GCACTCTTGA AAATGAAGAA CATAGTCAAA TAATTATCCA TTGTACACCT
    TCAACAGGTG CTTTTAAGCC CTGTGAATAT TTACTGGGAA GCTGGATGAT TCGTCTTACT
    GTGTGGTTCA TTTTCTTGGT TGCATTATTT TTCAACCTGC TTGTTATTTT AACAACATTT
    GCATCTTGTA CATCACTGCC TTCGTCCAAA TTGTTTATAG GCTTGATTTC TGTGTCTAAC
    TTATTCATGG GAATCTATAC TGGCATCCTA ACTTTTCTTG ATGCTGTGTC CTGGGGCAGA
    TTCGCTGAAT TTGGCATTTG GTGGGAAACT GGCAGTGGCT GCAAAGTAGC TGGGTTTCTT
    GCAGTTTTCT CCTCAGAAAG TGCCATATTT TTATTAATGC TAGCAACTGT CGAAAGAAGC
    TTATCTGCAA AAGATATAAT GAAAAATGGG AAGAGCAATC ATCTCAAACA GTTCCGGGTT
    GCTGCCCTTT TGGCTTTCCT AGGTGCTACA GTAGCAGGCT GTTTTCCCCT TTTCCATAGA
    GGGGAATATT CTGCATCACC CCTTTGTTTG CCATTTCCTA CAGGTGAAAC GCCATCATTA
    GGATTCACTG TAACGTTAGT GCTATTAAAC TCACTAGCAT TTTTATTAAT GGCCGTTATC
    TACACTAAGC TATACTGCAA CTTGGAAAAA GAGGACCTCT CAGAAAACTC ACAATCTAGC
    ATGATTAAGC ATGTCGCTTG GCTAATCTTC ACCAATTGCA TCTTTTTCTG CCCTGTGGCG
    TTTTTTTCAT TTGCACCATT GATCACTGCA ATCTCTATCA GCCCCGAAAT AATGAAGTCT
    GTTACTCTGA TATTTTTTCC ATTGCCTGCT TGCCTGAATC CAGTCCTGTA TGTTTTCTTC
    AACCCAAAGT TTAAAGAAGA CTGGAAGTTA CTGAAGCGAC GTGTTACCAA GAAAAGTGGA
    TCAGTTTCAG TTTCCATCAG TAGCCAAGGT GGTTGTCTGG AACAGGATTT CTACTACGAC
    TGTGGCATGT ACTCACATTT GCAGGGCAAC CTGACTGTTT GCGACTGCTG CGAATCGTTT
    CTTTTAACAA AGCCAGTATC ATGCAAACAC TTGATAAAAT CACACAGCTG TCCTGCATTG
    GCAGTGGCTT CTTGCCAAAG ACCTGAGGGC TACTGGTCCG ACTGTGGCAC ACAGTCGGCC
    CACTCTGATT ATGCAGATGA AGAAGATTCC TTTGTCTCAG ACAGTTCTGA CCAGGTGCAG
    GCCTGTGGAC GAGCCTGCTT CTACCAGAGT AGAGGATTCC CTTTGGTGCG CTATGCTTAC
    AATCTACCAA GAGTTAAAGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.