LGR4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LGR4
Entrez Gene ID	55366
Full Name	leucine rich repeat containing G protein-coupled receptor 4
Synonyms	BNMD17,GPR48
General protein information	Preferred Names leucine rich repeat containing G protein-coupled receptor 4 Names leucine-rich repeat-containing G-protein coupled receptor 4 G protein-coupled receptor 48
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p14.1
Summary	The protein encoded by this gene is a G-protein coupled receptor that binds R-spondins and activates the Wnt signaling pathway. This Wnt signaling pathway activation is necessary for proper development of many organs of the body. [provided by RefSeq, Oct 2016].
Disorder MIM:	606666
Disorder Html:	{Bone mineral density, low, susceptibility to}, 615311 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_018490.3	NP_060960.2	leucine-rich repeat-containing G-protein coupled receptor 4 isoform 1 precursor
NM_018490.2	NP_060960.2	leucine-rich repeat-containing G-protein coupled receptor 4 precursor
NM_001346432.1	NP_001333361.1	leucine-rich repeat-containing G-protein coupled receptor 4 isoform 2 precursor
NM_018490.4	NP_060960.2	leucine-rich repeat-containing G-protein coupled receptor 4 isoform 1 precursor
NM_018490.5	NP_060960.2	leucine-rich repeat-containing G-protein coupled receptor 4 isoform 1 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LGR4	XP_005633820.1
Pan troglodytes (chimpanzee)	LGR4	XP_003313024.2
Homo sapiens (human)	LGR4	NP_060960.2
Rattus norvegicus (Norway rat)	Lgr4	NP_775450.1
	LGR4	XP_001090038.2
Bos taurus (cattle)	LGR4	NP_001192440.1
Mus musculus (house mouse)	Lgr4	NP_766259.2
Gallus gallus (chicken)	LGR4	XP_426162.3
Xenopus tropicalis (tropical clawed frog)	lgr4	NP_001119970.1
Danio rerio (zebrafish)	lgr4	XP_687184.4

Gene Ontology
Bibliography

Related articles in PubMed

LGR4 modulates breast cancer initiation, metastasis, and cancer stem cells.
Yue Z, Yuan Z, Zeng L, Wang Y, Lai L, Li J, Sun P, Xue X, Qi J, Yang Z, Zheng Y, Fang Y, Li D, Siwko S, Li Y, Luo J, Liu M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the host protein leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4) is essential for VSV and VSV-G pseudotyped lentivirus (VSVG-LV) to infect susceptible cells.
Title: Leucine-rich repeat-containing G protein-coupled receptor 4 facilitates vesicular stomatitis virus infection by binding vesicular stomatitis virus glycoprotein.

Data (including data from studies using transgenic/knockout mice) suggest that LGR4 is key protein necessary for prostate cancer epithelial-mesenchymal transition and metastasis; LGR4 expression is elevated in human prostate cancer cell lines with metastatic potential; LGR4 silencing in prostate cancer cell line impairs cell migration and invasion without affecting cell proliferation.
Title: Leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4) is necessary for prostate cancer metastasis via epithelial-mesenchymal transition.

High LGR4 expression is associated with osteoarthritis.
Title: Alteration of Wnt5a expression and of the non-canonical Wnt/PCP and Wnt/PKC-Ca2+ pathways in human osteoarthritis osteoblasts.

The LGR4 A750T variant is correlated with central obesity-related characteristics in young subjects.
Title: Association of a gain-of-function variant in LGR4 with central obesity.

miR-34a gene expression upregulation inhibits ARPE-19 cell proliferation, migration and adhesion partly by suppressing LGR4 expression.
Title: LGR4 Is a Direct Target of MicroRNA-34a and Modulates the Proliferation and Migration of Retinal Pigment Epithelial ARPE-19 Cells.

The following LGR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LGR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27175	NM_018490.4 Latest version!	Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu109417	NM_018490.5 Latest version!	Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu100713	NM_001346432.1 Latest version!	Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu27175	NM_018490.2	Homo sapiens leucine-rich repeat containing G protein-coupled receptor 4 (LGR4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu27175	NM_018490.3	Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LGR4 ( NM_018490.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060960.2 Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27175
Clone ID Related Accession (Same CDS sequence)	NM_018490.3 , NM_018490.2 , NM_018490.4
Accession Version	NM_018490.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-09
Organism	Homo sapiens(human)
Product	leucine-rich repeat-containing G-protein coupled receptor 4 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC033039.2, DR000664.1, AB209743.1, AF257182.1 and AC090597.9. This sequence is a reference standard in the RefSeqGene project. On Oct 12, 2016 this sequence version replaced NM_018490.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF257182.1, SRR1803614.192490.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA 
GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT 
CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC 
TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA 
CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT 
ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG 
GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG 
GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT 
TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT 
TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT 
CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT 
TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT 
GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC 
CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC 
CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA 
GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT 
TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA 
AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG 
ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA 
AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA 
CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG 
TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA 
AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT 
GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC 
CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG 
ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT 
TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT 
TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG 
TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA 
GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT 
GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA 
CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC 
CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA 
ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA 
ATGGCCGTTA TCTACACTAA GCTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC 
TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC 
TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA 
ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG 
TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC 
AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT 
TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC 
TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC 
TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC 
ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT 
GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG 
CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060960.2
CDS	474..3329
Translation	MPGPLGLLCFLALGLLGSAGPSGAAPPLCAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALDISMNNITQLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAIKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPLSFVGNSAFHNLSDLHSLVIRGASMVQQFPNLTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRNLIHEIHSRAFATLGPITNLDVSFNELTSFPTEGLNGLNQLKLVGNFKLKEALAAKDFVNLRSLSVPYAYQCCAFWGCDSYANLNTEDNSLQDHSVAQEKGTADAANVTSTLENEEHSQIIIHCTPSTGAFKPCEYLLGSWMIRLTVWFIFLVALFFNLLVILTTFASCTSLPSSKLFIGLISVSNLFMGIYTGILTFLDAVSWGRFAEFGIWWETGSGCKVAGFLAVFSSESAIFLLMLATVERSLSAKDIMKNGKSNHLKQFRVAALLAFLGATVAGCFPLFHRGEYSASPLCLPFPTGETPSLGFTVTLVLLNSLAFLLMAVIYTKLYCNLEKEDLSENSQSSMIKHVAWLIFTNCIFFCPVAFFSFAPLITAISISPEIMKSVTLIFFPLPACLNPVLYVFFNPKFKEDWKLLKRRVTKKSGSVSVSISSQGGCLEQDFYYDCGMYSHLQGNLTVCDCCESFLLTKPVSCKHLIKSHSCPALAVASCQRPEGYWSDCGTQSAHSDYADEEDSFVSDSSDQVQACGRACFYQSRGFPLVRYAYNLPRVKD

Target ORF information:

RefSeq Version	NM_018490.3
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018490.3

ORF Insert Sequence:

ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA 
GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT 
CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC 
TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA 
CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT 
ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG 
GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG 
GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT 
TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT 
TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT 
CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT 
TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT 
GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC 
CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC 
CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA 
GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT 
TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA 
AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG 
ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA 
AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA 
CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG 
TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA 
AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT 
GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC 
CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG 
ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT 
TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT 
TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG 
TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA 
GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT 
GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA 
CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC 
CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA 
ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA 
ATGGCCGTTA TCTACACTAA GCTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC 
TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC 
TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA 
ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG 
TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC 
AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT 
TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC 
TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC 
TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC 
ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT 
GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG 
CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LGR4 ( NM_018490.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060960.2 Homo sapiens leucine-rich repeat containing G protein-coupled receptor 4 (LGR4), mRNA.

Price & Availability↑

CloneID	OHu27175
Clone ID Related Accession (Same CDS sequence)	NM_018490.3 , NM_018490.2 , NM_018490.4
Accession Version	NM_018490.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	leucine-rich repeat-containing G-protein coupled receptor 4 precursor
Comment

ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA 
GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT 
CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC 
TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA 
CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT 
ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG 
GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG 
GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT 
TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT 
TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT 
CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT 
TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT 
GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC 
CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC 
CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA 
GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT 
TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA 
AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG 
ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA 
AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA 
CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG 
TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA 
AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT 
GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC 
CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG 
ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT 
TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT 
TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG 
TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA 
GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT 
GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA 
CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC 
CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA 
ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA 
ATGGCCGTTA TCTACACTAA GCTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC 
TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC 
TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA 
ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG 
TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC 
AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT 
TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC 
TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC 
TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC 
ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT 
GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG 
CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_060960.2

Translation

MPGPLGLLCFLALGLLGSAGPSGAAPPLCAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALDISMNNITQLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAIKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPLSFVGNSAFHNLSDLHSLVIRGASMVQQFPNLTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRNLIHEIHSRAFATLGPITNLDVSFNELTSFPTEGLNGLNQLKLVGNFKLKEALAAKDFVNLRSLSVPYAYQCCAFWGCDSYANLNTEDNSLQDHSVAQEKGTADAANVTSTLENEEHSQIIIHCTPSTGAFKPCEYLLGSWMIRLTVWFIFLVALFFNLLVILTTFASCTSLPSSKLFIGLISVSNLFMGIYTGILTFLDAVSWGRFAEFGIWWETGSGCKVAGFLAVFSSESAIFLLMLATVERSLSAKDIMKNGKSNHLKQFRVAALLAFLGATVAGCFPLFHRGEYSASPLCLPFPTGETPSLGFTVTLVLLNSLAFLLMAVIYTKLYCNLEKEDLSENSQSSMIKHVAWLIFTNCIFFCPVAFFSFAPLITAISISPEIMKSVTLIFFPLPACLNPVLYVFFNPKFKEDWKLLKRRVTKKSGSVSVSISSQGGCLEQDFYYDCGMYSHLQGNLTVCDCCESFLLTKPVSCKHLIKSHSCPALAVASCQRPEGYWSDCGTQSAHSDYADEEDSFVSDSSDQVQACGRACFYQSRGFPLVRYAYNLPRVKD

Target ORF information:

RefSeq Version	NM_018490.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens leucine-rich repeat containing G protein-coupled receptor 4 (LGR4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018490.2

ORF Insert Sequence:

ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA 
GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT 
CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC 
TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA 
CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT 
ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG 
GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG 
GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT 
TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT 
TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT 
CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT 
TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT 
GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC 
CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC 
CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA 
GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT 
TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA 
AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG 
ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA 
AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA 
CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG 
TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA 
AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT 
GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC 
CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG 
ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT 
TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT 
TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG 
TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA 
GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT 
GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA 
CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC 
CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA 
ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA 
ATGGCCGTTA TCTACACTAA GCTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC 
TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC 
TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA 
ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG 
TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC 
AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT 
TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC 
TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC 
TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC 
ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT 
GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG 
CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LGR4 ( NM_018490.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060960.2 Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27175
Clone ID Related Accession (Same CDS sequence)	NM_018490.3 , NM_018490.2 , NM_018490.4
Accession Version	NM_018490.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Homo sapiens(human)
Product	leucine-rich repeat-containing G-protein coupled receptor 4 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC100771.2, BC033039.2, DR000664.1, AB209743.1, AF257182.1 and AC090597.9. On Nov 24, 2018 this sequence version replaced NM_018490.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF257182.1, SRR1803614.192490.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA 
GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT 
CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC 
TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA 
CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT 
ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG 
GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG 
GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT 
TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT 
TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT 
CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT 
TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT 
GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC 
CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC 
CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA 
GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT 
TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA 
AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG 
ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA 
AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA 
CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG 
TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA 
AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT 
GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC 
CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG 
ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT 
TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT 
TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG 
TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA 
GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT 
GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA 
CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC 
CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA 
ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA 
ATGGCCGTTA TCTACACTAA GCTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC 
TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC 
TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA 
ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG 
TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC 
AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT 
TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC 
TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC 
TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC 
ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT 
GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG 
CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060960.2
CDS	489..3344
Translation	MPGPLGLLCFLALGLLGSAGPSGAAPPLCAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALDISMNNITQLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAIKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPLSFVGNSAFHNLSDLHSLVIRGASMVQQFPNLTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRNLIHEIHSRAFATLGPITNLDVSFNELTSFPTEGLNGLNQLKLVGNFKLKEALAAKDFVNLRSLSVPYAYQCCAFWGCDSYANLNTEDNSLQDHSVAQEKGTADAANVTSTLENEEHSQIIIHCTPSTGAFKPCEYLLGSWMIRLTVWFIFLVALFFNLLVILTTFASCTSLPSSKLFIGLISVSNLFMGIYTGILTFLDAVSWGRFAEFGIWWETGSGCKVAGFLAVFSSESAIFLLMLATVERSLSAKDIMKNGKSNHLKQFRVAALLAFLGATVAGCFPLFHRGEYSASPLCLPFPTGETPSLGFTVTLVLLNSLAFLLMAVIYTKLYCNLEKEDLSENSQSSMIKHVAWLIFTNCIFFCPVAFFSFAPLITAISISPEIMKSVTLIFFPLPACLNPVLYVFFNPKFKEDWKLLKRRVTKKSGSVSVSISSQGGCLEQDFYYDCGMYSHLQGNLTVCDCCESFLLTKPVSCKHLIKSHSCPALAVASCQRPEGYWSDCGTQSAHSDYADEEDSFVSDSSDQVQACGRACFYQSRGFPLVRYAYNLPRVKD

Target ORF information:

RefSeq Version	NM_018490.4
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018490.4

ORF Insert Sequence:

ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA 
GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT 
CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC 
TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA 
CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT 
ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG 
GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG 
GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT 
TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT 
TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT 
CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT 
TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT 
GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC 
CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC 
CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA 
GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT 
TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA 
AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG 
ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA 
AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA 
CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG 
TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA 
AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT 
GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC 
CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG 
ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT 
TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT 
TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG 
TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA 
GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT 
GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA 
CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC 
CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA 
ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA 
ATGGCCGTTA TCTACACTAA GCTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC 
TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC 
TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA 
ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG 
TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC 
AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT 
TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC 
TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC 
TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC 
ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT 
GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG 
CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LGR4 ( NM_018490.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060960.2 Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu109417
Clone ID Related Accession (Same CDS sequence)	NM_018490.5
Accession Version	NM_018490.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	leucine-rich repeat-containing G-protein coupled receptor 4 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC100771.2 and AC090597.9. On Aug 31, 2019 this sequence version replaced NM_018490.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF257182.1, AB209743.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000379214.9/ ENSP00000368516.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA 
GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT 
CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC 
TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA 
CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT 
ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG 
GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG 
GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT 
TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT 
TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT 
CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT 
TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT 
GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC 
CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC 
CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA 
GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT 
TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA 
AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG 
ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA 
AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA 
CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG 
TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA 
AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT 
GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC 
CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG 
ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT 
TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT 
TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG 
TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA 
GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT 
GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA 
CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC 
CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA 
ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA 
ATGGCCGTTA TCTACACTAA ACTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC 
TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC 
TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA 
ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG 
TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC 
AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT 
TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC 
TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC 
TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC 
ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT 
GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG 
CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060960.2
CDS	489..3344
Translation	MPGPLGLLCFLALGLLGSAGPSGAAPPLCAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALDISMNNITQLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAIKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPLSFVGNSAFHNLSDLHSLVIRGASMVQQFPNLTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRNLIHEIHSRAFATLGPITNLDVSFNELTSFPTEGLNGLNQLKLVGNFKLKEALAAKDFVNLRSLSVPYAYQCCAFWGCDSYANLNTEDNSLQDHSVAQEKGTADAANVTSTLENEEHSQIIIHCTPSTGAFKPCEYLLGSWMIRLTVWFIFLVALFFNLLVILTTFASCTSLPSSKLFIGLISVSNLFMGIYTGILTFLDAVSWGRFAEFGIWWETGSGCKVAGFLAVFSSESAIFLLMLATVERSLSAKDIMKNGKSNHLKQFRVAALLAFLGATVAGCFPLFHRGEYSASPLCLPFPTGETPSLGFTVTLVLLNSLAFLLMAVIYTKLYCNLEKEDLSENSQSSMIKHVAWLIFTNCIFFCPVAFFSFAPLITAISISPEIMKSVTLIFFPLPACLNPVLYVFFNPKFKEDWKLLKRRVTKKSGSVSVSISSQGGCLEQDFYYDCGMYSHLQGNLTVCDCCESFLLTKPVSCKHLIKSHSCPALAVASCQRPEGYWSDCGTQSAHSDYADEEDSFVSDSSDQVQACGRACFYQSRGFPLVRYAYNLPRVKD

Target ORF information:

RefSeq Version	NM_018490.5
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018490.5

ORF Insert Sequence:

ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA 
GCGCTGGATA TCAGTATGAA CAACATTACT CAGTTGCCAG AAGATGCATT TAAGAACTTT 
CCTTTTCTAG AAGAGCTACA ATTGGCGGGC AACGACCTTT CTTTTATCCA CCCAAAGGCC 
TTGTCTGGGT TGAAAGAACT CAAAGTTCTA ACGCTCCAGA ATAATCAGTT GAAAACAGTA 
CCCAGTGAAG CCATTCGAGG GCTGAGTGCT TTGCAGTCTT TGCGTTTAGA TGCCAACCAT 
ATTACCTCAG TCCCCGAGGA CAGTTTTGAA GGACTTGTTC AGTTACGGCA TCTGTGGCTG 
GATGACAACA GCTTGACGGA GGTGCCTGTG CACCCCCTCA GCAATCTGCC CACCCTACAG 
GCGCTGACCC TGGCTCTCAA CAAGATCTCA AGCATCCCTG ACTTTGCATT TACCAACCTT 
TCAAGCCTGG TAGTTCTGCA TCTTCATAAC AATAAAATTA GAAGCCTGAG TCAACACTGT 
TTTGATGGAC TAGATAACCT GGAGACCTTA GACTTGAATT ATAATAACTT GGGGGAATTT 
CCTCAGGCTA TTAAAGCCCT TCCTAGCCTT AAAGAGCTAG GATTTCATAG TAATTCTATT 
TCTGTTATCC CTGATGGAGC ATTTGATGGT AATCCACTCT TAAGAACTAT ACATTTGTAT 
GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ATTTATCTGA TCTTCATTCC 
CTAGTCATTC GTGGTGCAAG CATGGTGCAG CAGTTCCCCA ATCTTACAGG AACTGTCCAC 
CTGGAAAGTC TGACTTTGAC AGGTACAAAG ATAAGCAGCA TACCTAATAA TTTGTGTCAA 
GAACAAAAGA TGCTTAGGAC TTTGGACTTG TCTTACAATA ATATAAGAGA CCTTCCAAGT 
TTTAATGGTT GCCATGCTCT GGAAGAAATT TCTTTACAGC GTAATCAAAT CTACCAAATA 
AAGGAAGGCA CCTTTCAAGG CCTGATATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG 
ATACATGAAA TTCACAGTAG AGCTTTTGCC ACACTTGGGC CAATAACTAA CCTAGATGTA 
AGTTTCAATG AATTAACTTC CTTTCCTACG GAAGGCCTGA ATGGGCTAAA TCAACTGAAA 
CTTGTGGGCA ACTTCAAGCT GAAAGAAGCC TTAGCAGCAA AAGACTTTGT TAACCTCAGG 
TCTTTATCAG TACCATATGC TTATCAGTGC TGTGCATTTT GGGGTTGTGA CTCTTATGCA 
AATTTAAACA CAGAAGATAA CAGCCTCCAG GACCACAGTG TGGCACAGGA GAAAGGTACT 
GCTGATGCAG CAAATGTCAC AAGCACTCTT GAAAATGAAG AACATAGTCA AATAATTATC 
CATTGTACAC CTTCAACAGG TGCTTTTAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG 
ATTCGTCTTA CTGTGTGGTT CATTTTCTTG GTTGCATTAT TTTTCAACCT GCTTGTTATT 
TTAACAACAT TTGCATCTTG TACATCACTG CCTTCGTCCA AATTGTTTAT AGGCTTGATT 
TCTGTGTCTA ACTTATTCAT GGGAATCTAT ACTGGCATCC TAACTTTTCT TGATGCTGTG 
TCCTGGGGCA GATTCGCTGA ATTTGGCATT TGGTGGGAAA CTGGCAGTGG CTGCAAAGTA 
GCTGGGTTTC TTGCAGTTTT CTCCTCAGAA AGTGCCATAT TTTTATTAAT GCTAGCAACT 
GTCGAAAGAA GCTTATCTGC AAAAGATATA ATGAAAAATG GGAAGAGCAA TCATCTCAAA 
CAGTTCCGGG TTGCTGCCCT TTTGGCTTTC CTAGGTGCTA CAGTAGCAGG CTGTTTTCCC 
CTTTTCCATA GAGGGGAATA TTCTGCATCA CCCCTTTGTT TGCCATTTCC TACAGGTGAA 
ACGCCATCAT TAGGATTCAC TGTAACGTTA GTGCTATTAA ACTCACTAGC ATTTTTATTA 
ATGGCCGTTA TCTACACTAA ACTATACTGC AACTTGGAAA AAGAGGACCT CTCAGAAAAC 
TCACAATCTA GCATGATTAA GCATGTCGCT TGGCTAATCT TCACCAATTG CATCTTTTTC 
TGCCCTGTGG CGTTTTTTTC ATTTGCACCA TTGATCACTG CAATCTCTAT CAGCCCCGAA 
ATAATGAAGT CTGTTACTCT GATATTTTTT CCATTGCCTG CTTGCCTGAA TCCAGTCCTG 
TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGT TACTGAAGCG ACGTGTTACC 
AAGAAAAGTG GATCAGTTTC AGTTTCCATC AGTAGCCAAG GTGGTTGTCT GGAACAGGAT 
TTCTACTACG ACTGTGGCAT GTACTCACAT TTGCAGGGCA ACCTGACTGT TTGCGACTGC 
TGCGAATCGT TTCTTTTAAC AAAGCCAGTA TCATGCAAAC ACTTGATAAA ATCACACAGC 
TGTCCTGCAT TGGCAGTGGC TTCTTGCCAA AGACCTGAGG GCTACTGGTC CGACTGTGGC 
ACACAGTCGG CCCACTCTGA TTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGTTCT 
GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTAGAGGATT CCCTTTGGTG 
CGCTATGCTT ACAATCTACC AAGAGTTAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LGR4 ( NM_001346432.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333361.1 Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 2, mRNA.

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CloneID	OHu100713
Clone ID Related Accession (Same CDS sequence)	NM_001346432.1
Accession Version	NM_001346432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	leucine-rich repeat-containing G-protein coupled receptor 4 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC033039.2, DR000664.1, AB209743.1, AF257182.1 and AC090597.9. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033039.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA 
GCGCTACAAT TGGCGGGCAA CGACCTTTCT TTTATCCACC CAAAGGCCTT GTCTGGGTTG 
AAAGAACTCA AAGTTCTAAC GCTCCAGAAT AATCAGTTGA AAACAGTACC CAGTGAAGCC 
ATTCGAGGGC TGAGTGCTTT GCAGTCTTTG CGTTTAGATG CCAACCATAT TACCTCAGTC 
CCCGAGGACA GTTTTGAAGG ACTTGTTCAG TTACGGCATC TGTGGCTGGA TGACAACAGC 
TTGACGGAGG TGCCTGTGCA CCCCCTCAGC AATCTGCCCA CCCTACAGGC GCTGACCCTG 
GCTCTCAACA AGATCTCAAG CATCCCTGAC TTTGCATTTA CCAACCTTTC AAGCCTGGTA 
GTTCTGCATC TTCATAACAA TAAAATTAGA AGCCTGAGTC AACACTGTTT TGATGGACTA 
GATAACCTGG AGACCTTAGA CTTGAATTAT AATAACTTGG GGGAATTTCC TCAGGCTATT 
AAAGCCCTTC CTAGCCTTAA AGAGCTAGGA TTTCATAGTA ATTCTATTTC TGTTATCCCT 
GATGGAGCAT TTGATGGTAA TCCACTCTTA AGAACTATAC ATTTGTATGA TAATCCTCTG 
TCTTTTGTGG GGAACTCAGC ATTTCACAAT TTATCTGATC TTCATTCCCT AGTCATTCGT 
GGTGCAAGCA TGGTGCAGCA GTTCCCCAAT CTTACAGGAA CTGTCCACCT GGAAAGTCTG 
ACTTTGACAG GTACAAAGAT AAGCAGCATA CCTAATAATT TGTGTCAAGA ACAAAAGATG 
CTTAGGACTT TGGACTTGTC TTACAATAAT ATAAGAGACC TTCCAAGTTT TAATGGTTGC 
CATGCTCTGG AAGAAATTTC TTTACAGCGT AATCAAATCT ACCAAATAAA GGAAGGCACC 
TTTCAAGGCC TGATATCTCT AAGGATTCTA GATCTGAGTA GAAACCTGAT ACATGAAATT 
CACAGTAGAG CTTTTGCCAC ACTTGGGCCA ATAACTAACC TAGATGTAAG TTTCAATGAA 
TTAACTTCCT TTCCTACGGA AGGCCTGAAT GGGCTAAATC AACTGAAACT TGTGGGCAAC 
TTCAAGCTGA AAGAAGCCTT AGCAGCAAAA GACTTTGTTA ACCTCAGGTC TTTATCAGTA 
CCATATGCTT ATCAGTGCTG TGCATTTTGG GGTTGTGACT CTTATGCAAA TTTAAACACA 
GAAGATAACA GCCTCCAGGA CCACAGTGTG GCACAGGAGA AAGGTACTGC TGATGCAGCA 
AATGTCACAA GCACTCTTGA AAATGAAGAA CATAGTCAAA TAATTATCCA TTGTACACCT 
TCAACAGGTG CTTTTAAGCC CTGTGAATAT TTACTGGGAA GCTGGATGAT TCGTCTTACT 
GTGTGGTTCA TTTTCTTGGT TGCATTATTT TTCAACCTGC TTGTTATTTT AACAACATTT 
GCATCTTGTA CATCACTGCC TTCGTCCAAA TTGTTTATAG GCTTGATTTC TGTGTCTAAC 
TTATTCATGG GAATCTATAC TGGCATCCTA ACTTTTCTTG ATGCTGTGTC CTGGGGCAGA 
TTCGCTGAAT TTGGCATTTG GTGGGAAACT GGCAGTGGCT GCAAAGTAGC TGGGTTTCTT 
GCAGTTTTCT CCTCAGAAAG TGCCATATTT TTATTAATGC TAGCAACTGT CGAAAGAAGC 
TTATCTGCAA AAGATATAAT GAAAAATGGG AAGAGCAATC ATCTCAAACA GTTCCGGGTT 
GCTGCCCTTT TGGCTTTCCT AGGTGCTACA GTAGCAGGCT GTTTTCCCCT TTTCCATAGA 
GGGGAATATT CTGCATCACC CCTTTGTTTG CCATTTCCTA CAGGTGAAAC GCCATCATTA 
GGATTCACTG TAACGTTAGT GCTATTAAAC TCACTAGCAT TTTTATTAAT GGCCGTTATC 
TACACTAAGC TATACTGCAA CTTGGAAAAA GAGGACCTCT CAGAAAACTC ACAATCTAGC 
ATGATTAAGC ATGTCGCTTG GCTAATCTTC ACCAATTGCA TCTTTTTCTG CCCTGTGGCG 
TTTTTTTCAT TTGCACCATT GATCACTGCA ATCTCTATCA GCCCCGAAAT AATGAAGTCT 
GTTACTCTGA TATTTTTTCC ATTGCCTGCT TGCCTGAATC CAGTCCTGTA TGTTTTCTTC 
AACCCAAAGT TTAAAGAAGA CTGGAAGTTA CTGAAGCGAC GTGTTACCAA GAAAAGTGGA 
TCAGTTTCAG TTTCCATCAG TAGCCAAGGT GGTTGTCTGG AACAGGATTT CTACTACGAC 
TGTGGCATGT ACTCACATTT GCAGGGCAAC CTGACTGTTT GCGACTGCTG CGAATCGTTT 
CTTTTAACAA AGCCAGTATC ATGCAAACAC TTGATAAAAT CACACAGCTG TCCTGCATTG 
GCAGTGGCTT CTTGCCAAAG ACCTGAGGGC TACTGGTCCG ACTGTGGCAC ACAGTCGGCC 
CACTCTGATT ATGCAGATGA AGAAGATTCC TTTGTCTCAG ACAGTTCTGA CCAGGTGCAG 
GCCTGTGGAC GAGCCTGCTT CTACCAGAGT AGAGGATTCC CTTTGGTGCG CTATGCTTAC 
AATCTACCAA GAGTTAAAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333361.1
CDS	474..3257
Translation	MPGPLGLLCFLALGLLGSAGPSGAAPPLCAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAIKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPLSFVGNSAFHNLSDLHSLVIRGASMVQQFPNLTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRNLIHEIHSRAFATLGPITNLDVSFNELTSFPTEGLNGLNQLKLVGNFKLKEALAAKDFVNLRSLSVPYAYQCCAFWGCDSYANLNTEDNSLQDHSVAQEKGTADAANVTSTLENEEHSQIIIHCTPSTGAFKPCEYLLGSWMIRLTVWFIFLVALFFNLLVILTTFASCTSLPSSKLFIGLISVSNLFMGIYTGILTFLDAVSWGRFAEFGIWWETGSGCKVAGFLAVFSSESAIFLLMLATVERSLSAKDIMKNGKSNHLKQFRVAALLAFLGATVAGCFPLFHRGEYSASPLCLPFPTGETPSLGFTVTLVLLNSLAFLLMAVIYTKLYCNLEKEDLSENSQSSMIKHVAWLIFTNCIFFCPVAFFSFAPLITAISISPEIMKSVTLIFFPLPACLNPVLYVFFNPKFKEDWKLLKRRVTKKSGSVSVSISSQGGCLEQDFYYDCGMYSHLQGNLTVCDCCESFLLTKPVSCKHLIKSHSCPALAVASCQRPEGYWSDCGTQSAHSDYADEEDSFVSDSSDQVQACGRACFYQSRGFPLVRYAYNLPRVKD

Target ORF information:

RefSeq Version	NM_001346432.1
Organism	Homo sapiens(human)
Definition	Homo sapiens leucine rich repeat containing G protein-coupled receptor 4 (LGR4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346432.1

ORF Insert Sequence:

ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGGAAGGG GCTGACGGCC GTGCCCGAGG GGCTCAGCGC CTTCACCCAA 
GCGCTACAAT TGGCGGGCAA CGACCTTTCT TTTATCCACC CAAAGGCCTT GTCTGGGTTG 
AAAGAACTCA AAGTTCTAAC GCTCCAGAAT AATCAGTTGA AAACAGTACC CAGTGAAGCC 
ATTCGAGGGC TGAGTGCTTT GCAGTCTTTG CGTTTAGATG CCAACCATAT TACCTCAGTC 
CCCGAGGACA GTTTTGAAGG ACTTGTTCAG TTACGGCATC TGTGGCTGGA TGACAACAGC 
TTGACGGAGG TGCCTGTGCA CCCCCTCAGC AATCTGCCCA CCCTACAGGC GCTGACCCTG 
GCTCTCAACA AGATCTCAAG CATCCCTGAC TTTGCATTTA CCAACCTTTC AAGCCTGGTA 
GTTCTGCATC TTCATAACAA TAAAATTAGA AGCCTGAGTC AACACTGTTT TGATGGACTA 
GATAACCTGG AGACCTTAGA CTTGAATTAT AATAACTTGG GGGAATTTCC TCAGGCTATT 
AAAGCCCTTC CTAGCCTTAA AGAGCTAGGA TTTCATAGTA ATTCTATTTC TGTTATCCCT 
GATGGAGCAT TTGATGGTAA TCCACTCTTA AGAACTATAC ATTTGTATGA TAATCCTCTG 
TCTTTTGTGG GGAACTCAGC ATTTCACAAT TTATCTGATC TTCATTCCCT AGTCATTCGT 
GGTGCAAGCA TGGTGCAGCA GTTCCCCAAT CTTACAGGAA CTGTCCACCT GGAAAGTCTG 
ACTTTGACAG GTACAAAGAT AAGCAGCATA CCTAATAATT TGTGTCAAGA ACAAAAGATG 
CTTAGGACTT TGGACTTGTC TTACAATAAT ATAAGAGACC TTCCAAGTTT TAATGGTTGC 
CATGCTCTGG AAGAAATTTC TTTACAGCGT AATCAAATCT ACCAAATAAA GGAAGGCACC 
TTTCAAGGCC TGATATCTCT AAGGATTCTA GATCTGAGTA GAAACCTGAT ACATGAAATT 
CACAGTAGAG CTTTTGCCAC ACTTGGGCCA ATAACTAACC TAGATGTAAG TTTCAATGAA 
TTAACTTCCT TTCCTACGGA AGGCCTGAAT GGGCTAAATC AACTGAAACT TGTGGGCAAC 
TTCAAGCTGA AAGAAGCCTT AGCAGCAAAA GACTTTGTTA ACCTCAGGTC TTTATCAGTA 
CCATATGCTT ATCAGTGCTG TGCATTTTGG GGTTGTGACT CTTATGCAAA TTTAAACACA 
GAAGATAACA GCCTCCAGGA CCACAGTGTG GCACAGGAGA AAGGTACTGC TGATGCAGCA 
AATGTCACAA GCACTCTTGA AAATGAAGAA CATAGTCAAA TAATTATCCA TTGTACACCT 
TCAACAGGTG CTTTTAAGCC CTGTGAATAT TTACTGGGAA GCTGGATGAT TCGTCTTACT 
GTGTGGTTCA TTTTCTTGGT TGCATTATTT TTCAACCTGC TTGTTATTTT AACAACATTT 
GCATCTTGTA CATCACTGCC TTCGTCCAAA TTGTTTATAG GCTTGATTTC TGTGTCTAAC 
TTATTCATGG GAATCTATAC TGGCATCCTA ACTTTTCTTG ATGCTGTGTC CTGGGGCAGA 
TTCGCTGAAT TTGGCATTTG GTGGGAAACT GGCAGTGGCT GCAAAGTAGC TGGGTTTCTT 
GCAGTTTTCT CCTCAGAAAG TGCCATATTT TTATTAATGC TAGCAACTGT CGAAAGAAGC 
TTATCTGCAA AAGATATAAT GAAAAATGGG AAGAGCAATC ATCTCAAACA GTTCCGGGTT 
GCTGCCCTTT TGGCTTTCCT AGGTGCTACA GTAGCAGGCT GTTTTCCCCT TTTCCATAGA 
GGGGAATATT CTGCATCACC CCTTTGTTTG CCATTTCCTA CAGGTGAAAC GCCATCATTA 
GGATTCACTG TAACGTTAGT GCTATTAAAC TCACTAGCAT TTTTATTAAT GGCCGTTATC 
TACACTAAGC TATACTGCAA CTTGGAAAAA GAGGACCTCT CAGAAAACTC ACAATCTAGC 
ATGATTAAGC ATGTCGCTTG GCTAATCTTC ACCAATTGCA TCTTTTTCTG CCCTGTGGCG 
TTTTTTTCAT TTGCACCATT GATCACTGCA ATCTCTATCA GCCCCGAAAT AATGAAGTCT 
GTTACTCTGA TATTTTTTCC ATTGCCTGCT TGCCTGAATC CAGTCCTGTA TGTTTTCTTC 
AACCCAAAGT TTAAAGAAGA CTGGAAGTTA CTGAAGCGAC GTGTTACCAA GAAAAGTGGA 
TCAGTTTCAG TTTCCATCAG TAGCCAAGGT GGTTGTCTGG AACAGGATTT CTACTACGAC 
TGTGGCATGT ACTCACATTT GCAGGGCAAC CTGACTGTTT GCGACTGCTG CGAATCGTTT 
CTTTTAACAA AGCCAGTATC ATGCAAACAC TTGATAAAAT CACACAGCTG TCCTGCATTG 
GCAGTGGCTT CTTGCCAAAG ACCTGAGGGC TACTGGTCCG ACTGTGGCAC ACAGTCGGCC 
CACTCTGATT ATGCAGATGA AGAAGATTCC TTTGTCTCAG ACAGTTCTGA CCAGGTGCAG 
GCCTGTGGAC GAGCCTGCTT CTACCAGAGT AGAGGATTCC CTTTGGTGCG CTATGCTTAC 
AATCTACCAA GAGTTAAAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LGR4 modulates breast cancer initiation, metastasis, and cancer stem cells.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2017 Dec)
Yue Z,Yuan Z,Zeng L,Wang Y,Lai L,Li J,Sun P,Xue X,Qi J,Yang Z,Zheng Y,Fang Y,Li D,Siwko S,Li Y,Luo J,Liu M

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LGR4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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