HEMGN cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HEMGN
Entrez Gene ID	55363
Full Name	hemogen
Synonyms	CT155,EDAG,EDAG-1,NDR
General protein information	Preferred Names hemogen Names hemogen erythroid differentiation-associated gene protein hemopoietic gene protein negative differentiation regulator protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q22.33
Disorder MIM:	610715

mRNA and Protein(s)

mRNA	Protein	Name
NM_197978.2	NP_932095.1	hemogen
XM_011518846.2	XP_011517148.1	hemogen isoform X1
XM_005252086.1	XP_005252143.1	hemogen isoform X1
XM_011518845.2	XP_011517147.1	hemogen isoform X1
NM_018437.4	NP_060907.2	hemogen
NM_018437.5	NP_060907.2	hemogen
NM_197978.3	NP_932095.1	hemogen

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HEMGN	XP_001156499.1
Canis lupus familiaris (dog)	HEMGN	XP_005626908.1
Homo sapiens (human)	HEMGN	NP_932095.1
Bos taurus (cattle)	HEMGN	NP_001070523.1
Mus musculus (house mouse)	Hemgn	NP_444379.1
Rattus norvegicus (Norway rat)	Hemgn	NP_579828.1
	HEMGN	XP_001113799.1

Gene Ontology
Bibliography

Related articles in PubMed

EDAG promotes the expansion and survival of human CD34+ cells.
Zhao K, Zheng WW, Dong XM, Yin RH, Gao R, Li X, Liu JF, Zhan YQ, Yu M, Chen H, Ge CH, Ning HM, Yang XM, Li CY
PloS one13(1)e0190794(2018)

Embryonic develop-associated gene 1 is overexpressed and acts as a tumor promoter in thyroid carcinoma.
Xu J, Li Z, Su Q, Zhao J, Ma J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie8186-92(2016 Jul)

A 7-Gene Signature Depicts the Biochemical Profile of Early Prefibrotic Myelofibrosis.
Skov V, Burton M, Thomassen M, Stauffer Larsen T, Riley CH, Brinch Madelung A, Kj?r L, Bondo H, Stamp I, Ehinger M, Dahl-S?rensen R, Brochmann N, Nielsen K, Thiele J, Jensen MK, Weis Bjerrum O, Kruse TA, Hasselbalch HC
PloS one11(8)e0161570(2016)

EDAG positively regulates erythroid differentiation and modifies GATA1 acetylation through recruiting p300.
Zheng WW, Dong XM, Yin RH, Xu FF, Ning HM, Zhang MJ, Xu CW, Yang Y, Ding YL, Wang ZD, Zhao WB, Tang LJ, Chen H, Wang XH, Zhan YQ, Yu M, Ge CH, Li CY, Yang XM
Stem cells (Dayton, Ohio)32(8)2278-89(2014 Aug)

Erythroid differentiation-associated gene interacts with NPM1 (nucleophosmin/B23) and increases its protein stability, resisting cell apoptosis.
Zhang MJ, Ding YL, Xu CW, Yang Y, Lian WX, Zhan YQ, Li W, Xu WX, Yu M, Ge CH, Ning HM, Li CY, Yang XM
The FEBS journal279(16)2848-62(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EDAG-1 regulates the proliferation and apoptosis of thyroid carcinoma via the PI3K/Akt signaling pathway.
Title: Embryonic develop-associated gene 1 is overexpressed and acts as a tumor promoter in thyroid carcinoma.

a 7-gene signature was identified which correctly predicted the primary prefibrotic myelofibrosis group with a sensitivity of 100% and a specificity of 89%. The 7 genes included MPO, CEACAM8, CRISP3, MS4A3, CEACAM6, HEMGN, and MMP8
Title: A 7-Gene Signature Depicts the Biochemical Profile of Early Prefibrotic Myelofibrosis.

EDAG forms complex with GATA1 and p300 and increases GATA1 acetylation and transcriptional activity by facilitating the interaction between GATA1 and p300
Title: EDAG positively regulates erythroid differentiation and modifies GATA1 acetylation through recruiting p300.

EDAG enhances the protein stability of NPM1 via binding to NPM1, which plays a critical role in the anti-apoptosis of leukaemia cells.
Title: Erythroid differentiation-associated gene interacts with NPM1 (nucleophosmin/B23) and increases its protein stability, resisting cell apoptosis.

EDAG functions as a positive regulator of erythroid/megakaryocytic differentiation in 32D cells associated with the induction of GATA-1 and its target genes
Title: Over-expression of EDAG in the myeloid cell line 32D: induction of GATA-1 expression and erythroid/megakaryocytic phenotype.

The following HEMGN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEMGN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02454	NM_018437.5 Latest version!	Homo sapiens hemogen (HEMGN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu02454	NM_197978.3 Latest version!	Homo sapiens hemogen (HEMGN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu02454	XM_005252086.1 Latest version!	Homo sapiens hemogen (HEMGN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu02454	XM_011518845.2 Latest version!	Homo sapiens hemogen (HEMGN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu02454	XM_011518846.2 Latest version!	Homo sapiens hemogen (HEMGN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu02454	NM_018437.4	Homo sapiens hemogen (HEMGN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu02454	NM_197978.2	Homo sapiens hemogen (HEMGN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEMGN ( NM_018437.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060907.2 Homo sapiens hemogen (HEMGN), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02454
Clone ID Related Accession (Same CDS sequence)	NM_197978.2 , XM_011518846.2 , XM_005252086.1 , XM_011518845.2 , NM_018437.4 , NM_018437.5 , NM_197978.3
Accession Version	NM_018437.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	hemogen
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY026328.1, AF322875.1 and AF306682.1. On May 31, 2019 this sequence version replaced NM_018437.4. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF228713.2, AF322875.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2148874 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060907.2
CDS	141..1595
Translation	MDLGKDQSHLKHHQTPDPHQEENHSPEVIGTWSLRNRELLRKRKAEVHEKETSQWLFGEQKKRKQQRTGKGNRRGRKRQQNTELKVEPQPQIEKEIVEKALAPIEKKTEPPGSITKVFPSVASPQKVVPEEHFSEICQESNIYQENFSEYQEIAVQNHSSETCQHVSEPEDLSPKMYQEISVLQDNSSKICQDMKEPEDNSPNTCQVISVIQDHPFKMYQDMAKREDLAPKMCQEAAVPKILPCPTSEDTADLAGCSLQAYPKPDVPKGYILDTDQNPAEPEEYNETDQGIAETEGLFPKIQEIAEPKDLSTKTHQESAEPKYLPHKTCNEIIVPKAPSHKTIQETPHSEDYSIEINQETPGSEKYSPETYQEIPGLEEYSPEIYQETSQLEEYSPEIYQETPGPEDLSTETYKNKDVPKECFPEPHQETGGPQGQDPKAHQEDAKDAYTFPQEMKEKPKEEPGIPAILNESHPENDVYSYVLF

Target ORF information:

RefSeq Version	NM_018437.5
Organism	Homo sapiens(human)
Definition	Homo sapiens hemogen (HEMGN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018437.5

ORF Insert Sequence:

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEMGN ( NM_197978.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_932095.1 Homo sapiens hemogen (HEMGN), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02454
Clone ID Related Accession (Same CDS sequence)	NM_197978.2 , XM_011518846.2 , XM_005252086.1 , XM_011518845.2 , NM_018437.4 , NM_018437.5 , NM_197978.3
Accession Version	NM_197978.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	hemogen
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA485094.1 and AF306682.1. On May 21, 2013 this sequence version replaced NM_197978.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY244805.1, AY255672.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_932095.1
CDS	258..1712
Translation	MDLGKDQSHLKHHQTPDPHQEENHSPEVIGTWSLRNRELLRKRKAEVHEKETSQWLFGEQKKRKQQRTGKGNRRGRKRQQNTELKVEPQPQIEKEIVEKALAPIEKKTEPPGSITKVFPSVASPQKVVPEEHFSEICQESNIYQENFSEYQEIAVQNHSSETCQHVSEPEDLSPKMYQEISVLQDNSSKICQDMKEPEDNSPNTCQVISVIQDHPFKMYQDMAKREDLAPKMCQEAAVPKILPCPTSEDTADLAGCSLQAYPKPDVPKGYILDTDQNPAEPEEYNETDQGIAETEGLFPKIQEIAEPKDLSTKTHQESAEPKYLPHKTCNEIIVPKAPSHKTIQETPHSEDYSIEINQETPGSEKYSPETYQEIPGLEEYSPEIYQETSQLEEYSPEIYQETPGPEDLSTETYKNKDVPKECFPEPHQETGGPQGQDPKAHQEDAKDAYTFPQEMKEKPKEEPGIPAILNESHPENDVYSYVLF

Target ORF information:

RefSeq Version	NM_197978.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hemogen (HEMGN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_197978.2

ORF Insert Sequence:

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEMGN ( NM_018437.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060907.2 Homo sapiens hemogen (HEMGN), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02454
Clone ID Related Accession (Same CDS sequence)	NM_197978.2 , XM_011518846.2 , XM_005252086.1 , XM_011518845.2 , NM_018437.4 , NM_018437.5 , NM_197978.3
Accession Version	NM_018437.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	hemogen
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY026328.1, AF322875.1 and AF306682.1. On May 21, 2013 this sequence version replaced NM_018437.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF228713.2, AK292441.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2148874 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060907.2
CDS	204..1658
Translation	MDLGKDQSHLKHHQTPDPHQEENHSPEVIGTWSLRNRELLRKRKAEVHEKETSQWLFGEQKKRKQQRTGKGNRRGRKRQQNTELKVEPQPQIEKEIVEKALAPIEKKTEPPGSITKVFPSVASPQKVVPEEHFSEICQESNIYQENFSEYQEIAVQNHSSETCQHVSEPEDLSPKMYQEISVLQDNSSKICQDMKEPEDNSPNTCQVISVIQDHPFKMYQDMAKREDLAPKMCQEAAVPKILPCPTSEDTADLAGCSLQAYPKPDVPKGYILDTDQNPAEPEEYNETDQGIAETEGLFPKIQEIAEPKDLSTKTHQESAEPKYLPHKTCNEIIVPKAPSHKTIQETPHSEDYSIEINQETPGSEKYSPETYQEIPGLEEYSPEIYQETSQLEEYSPEIYQETPGPEDLSTETYKNKDVPKECFPEPHQETGGPQGQDPKAHQEDAKDAYTFPQEMKEKPKEEPGIPAILNESHPENDVYSYVLF

Target ORF information:

RefSeq Version	NM_018437.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hemogen (HEMGN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018437.4

ORF Insert Sequence:

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEMGN ( NM_197978.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_932095.1 Homo sapiens hemogen (HEMGN), transcript variant 1, mRNA.

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CloneID	OHu02454
Clone ID Related Accession (Same CDS sequence)	NM_197978.2 , XM_011518846.2 , XM_005252086.1 , XM_011518845.2 , NM_018437.4 , NM_018437.5 , NM_197978.3
Accession Version	NM_197978.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	hemogen
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF306682.1. On Nov 23, 2018 this sequence version replaced NM_197978.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF306682.1, AY255672.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000616898.2/ ENSP00000480020.1 RefSeq Select criteria :: based on expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_932095.1
CDS	37..1491
Translation	MDLGKDQSHLKHHQTPDPHQEENHSPEVIGTWSLRNRELLRKRKAEVHEKETSQWLFGEQKKRKQQRTGKGNRRGRKRQQNTELKVEPQPQIEKEIVEKALAPIEKKTEPPGSITKVFPSVASPQKVVPEEHFSEICQESNIYQENFSEYQEIAVQNHSSETCQHVSEPEDLSPKMYQEISVLQDNSSKICQDMKEPEDNSPNTCQVISVIQDHPFKMYQDMAKREDLAPKMCQEAAVPKILPCPTSEDTADLAGCSLQAYPKPDVPKGYILDTDQNPAEPEEYNETDQGIAETEGLFPKIQEIAEPKDLSTKTHQESAEPKYLPHKTCNEIIVPKAPSHKTIQETPHSEDYSIEINQETPGSEKYSPETYQEIPGLEEYSPEIYQETSQLEEYSPEIYQETPGPEDLSTETYKNKDVPKECFPEPHQETGGPQGQDPKAHQEDAKDAYTFPQEMKEKPKEEPGIPAILNESHPENDVYSYVLF

Target ORF information:

RefSeq Version	NM_197978.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hemogen (HEMGN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_197978.3

ORF Insert Sequence:

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEMGN ( XM_011518846.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517148.1 Homo sapiens hemogen (HEMGN), transcript variant X3, mRNA.

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CloneID	OHu02454
Clone ID Related Accession (Same CDS sequence)	NM_197978.2 , XM_011518846.2 , XM_005252086.1 , XM_011518845.2 , NM_018437.4 , NM_018437.5 , NM_197978.3
Accession Version	XM_011518846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	hemogen isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518846.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517148.1
CDS	188..1642
Translation	MDLGKDQSHLKHHQTPDPHQEENHSPEVIGTWSLRNRELLRKRKAEVHEKETSQWLFGEQKKRKQQRTGKGNRRGRKRQQNTELKVEPQPQIEKEIVEKALAPIEKKTEPPGSITKVFPSVASPQKVVPEEHFSEICQESNIYQENFSEYQEIAVQNHSSETCQHVSEPEDLSPKMYQEISVLQDNSSKICQDMKEPEDNSPNTCQVISVIQDHPFKMYQDMAKREDLAPKMCQEAAVPKILPCPTSEDTADLAGCSLQAYPKPDVPKGYILDTDQNPAEPEEYNETDQGIAETEGLFPKIQEIAEPKDLSTKTHQESAEPKYLPHKTCNEIIVPKAPSHKTIQETPHSEDYSIEINQETPGSEKYSPETYQEIPGLEEYSPEIYQETSQLEEYSPEIYQETPGPEDLSTETYKNKDVPKECFPEPHQETGGPQGQDPKAHQEDAKDAYTFPQEMKEKPKEEPGIPAILNESHPENDVYSYVLF

Target ORF information:

RefSeq Version	XM_011518846.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hemogen (HEMGN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518846.2

ORF Insert Sequence:

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEMGN ( XM_005252086.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252143.1 Homo sapiens hemogen (HEMGN), transcript variant X1, mRNA.

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CloneID	OHu02454
Clone ID Related Accession (Same CDS sequence)	NM_197978.2 , XM_011518846.2 , XM_005252086.1 , XM_011518845.2 , NM_018437.4 , NM_018437.5 , NM_197978.3
Accession Version	XM_005252086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	hemogen isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252143.1
CDS	166..1620
Translation	MDLGKDQSHLKHHQTPDPHQEENHSPEVIGTWSLRNRELLRKRKAEVHEKETSQWLFGEQKKRKQQRTGKGNRRGRKRQQNTELKVEPQPQIEKEIVEKALAPIEKKTEPPGSITKVFPSVASPQKVVPEEHFSEICQESNIYQENFSEYQEIAVQNHSSETCQHVSEPEDLSPKMYQEISVLQDNSSKICQDMKEPEDNSPNTCQVISVIQDHPFKMYQDMAKREDLAPKMCQEAAVPKILPCPTSEDTADLAGCSLQAYPKPDVPKGYILDTDQNPAEPEEYNETDQGIAETEGLFPKIQEIAEPKDLSTKTHQESAEPKYLPHKTCNEIIVPKAPSHKTIQETPHSEDYSIEINQETPGSEKYSPETYQEIPGLEEYSPEIYQETSQLEEYSPEIYQETPGPEDLSTETYKNKDVPKECFPEPHQETGGPQGQDPKAHQEDAKDAYTFPQEMKEKPKEEPGIPAILNESHPENDVYSYVLF

Target ORF information:

RefSeq Version	XM_005252086.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hemogen (HEMGN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252086.1

ORF Insert Sequence:

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEMGN ( XM_011518845.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517147.1 Homo sapiens hemogen (HEMGN), transcript variant X2, mRNA.

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CloneID	OHu02454
Clone ID Related Accession (Same CDS sequence)	NM_197978.2 , XM_011518846.2 , XM_005252086.1 , XM_011518845.2 , NM_018437.4 , NM_018437.5 , NM_197978.3
Accession Version	XM_011518845.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	hemogen isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518845.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517147.1
CDS	78..1532
Translation	MDLGKDQSHLKHHQTPDPHQEENHSPEVIGTWSLRNRELLRKRKAEVHEKETSQWLFGEQKKRKQQRTGKGNRRGRKRQQNTELKVEPQPQIEKEIVEKALAPIEKKTEPPGSITKVFPSVASPQKVVPEEHFSEICQESNIYQENFSEYQEIAVQNHSSETCQHVSEPEDLSPKMYQEISVLQDNSSKICQDMKEPEDNSPNTCQVISVIQDHPFKMYQDMAKREDLAPKMCQEAAVPKILPCPTSEDTADLAGCSLQAYPKPDVPKGYILDTDQNPAEPEEYNETDQGIAETEGLFPKIQEIAEPKDLSTKTHQESAEPKYLPHKTCNEIIVPKAPSHKTIQETPHSEDYSIEINQETPGSEKYSPETYQEIPGLEEYSPEIYQETSQLEEYSPEIYQETPGPEDLSTETYKNKDVPKECFPEPHQETGGPQGQDPKAHQEDAKDAYTFPQEMKEKPKEEPGIPAILNESHPENDVYSYVLF

Target ORF information:

RefSeq Version	XM_011518845.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hemogen (HEMGN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011518845.2

ORF Insert Sequence:

ATGGATTTGG GAAAGGACCA ATCTCATTTG AAGCACCATC AGACACCTGA CCCTCATCAA 
GAAGAGAACC ATTCTCCAGA AGTCATTGGA ACCTGGAGTT TGAGAAACAG AGAACTACTT 
AGAAAAAGAA AAGCTGAAGT GCATGAAAAG GAAACATCAC AATGGCTATT TGGAGAACAG 
AAAAAACGCA AGCAGCAGAG AACAGGAAAA GGAAATCGAA GAGGCAGAAA GAGACAACAA 
AACACAGAAT TGAAGGTGGA GCCTCAGCCA CAGATAGAAA AGGAAATAGT GGAGAAAGCA 
CTGGCACCTA TAGAGAAAAA AACTGAGCCA CCTGGGAGCA TAACCAAAGT ATTTCCTTCA 
GTAGCCTCCC CGCAAAAAGT TGTGCCTGAG GAACACTTTT CTGAAATATG TCAAGAAAGT 
AACATATATC AGGAGAATTT TTCTGAGTAC CAAGAAATAG CAGTACAAAA CCATTCTTCT 
GAAACATGCC AACATGTGTC TGAACCTGAA GACCTCTCTC CTAAAATGTA CCAAGAAATA 
TCTGTACTTC AAGACAATTC TTCCAAAATA TGCCAAGACA TGAAGGAACC TGAAGACAAC 
TCTCCTAACA CATGCCAAGT AATATCTGTA ATTCAAGACC ATCCTTTCAA AATGTACCAA 
GATATGGCTA AACGAGAAGA TCTGGCTCCT AAAATGTGCC AAGAAGCTGC TGTACCCAAA 
ATCCTTCCTT GTCCAACATC TGAAGACACA GCTGATCTGG CAGGATGCTC TCTTCAAGCA 
TATCCAAAAC CAGATGTGCC TAAAGGCTAT ATTCTTGACA CAGACCAAAA TCCAGCAGAA 
CCAGAGGAAT ACAATGAAAC AGATCAAGGA ATAGCTGAGA CAGAAGGCCT TTTTCCTAAA 
ATACAAGAAA TAGCTGAGCC TAAAGACCTT TCTACAAAAA CACACCAAGA ATCAGCTGAA 
CCTAAATACC TTCCTCATAA AACATGTAAC GAAATTATTG TGCCTAAAGC CCCCTCTCAT 
AAAACAATCC AAGAAACACC TCATTCTGAA GACTATTCAA TTGAAATAAA CCAAGAAACT 
CCTGGGTCTG AAAAATATTC ACCTGAAACG TATCAAGAAA TACCTGGGCT TGAAGAATAT 
TCACCTGAAA TATACCAAGA AACATCCCAG CTTGAAGAAT ATTCACCTGA AATATACCAA 
GAAACACCGG GGCCTGAAGA CCTCTCTACT GAGACATATA AAAATAAGGA TGTGCCTAAA 
GAATGCTTTC CAGAACCACA CCAAGAAACA GGTGGGCCCC AAGGCCAGGA TCCTAAAGCA 
CACCAGGAAG ATGCTAAAGA TGCTTATACT TTTCCTCAAG AAATGAAAGA AAAACCCAAA 
GAAGAGCCAG GAATACCAGC AATTCTGAAT GAGAGTCATC CAGAAAATGA TGTCTATAGT 
TATGTTTTGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EDAG promotes the expansion and survival of human CD34+ cells.
PloS one13(1)e0190794(2018)
Zhao K,Zheng WW,Dong XM,Yin RH,Gao R,Li X,Liu JF,Zhan YQ,Yu M,Chen H,Ge CH,Ning HM,Yang XM,Li CY

Embryonic develop-associated gene 1 is overexpressed and acts as a tumor promoter in thyroid carcinoma.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie8186-92(2016 Jul)
Xu J,Li Z,Su Q,Zhao J,Ma J

A 7-Gene Signature Depicts the Biochemical Profile of Early Prefibrotic Myelofibrosis.
PloS one11(8)e0161570(2016)
Skov V,Burton M,Thomassen M,Stauffer Larsen T,Riley CH,Brinch Madelung A,Kj?r L,Bondo H,Stamp I,Ehinger M,Dahl-S?rensen R,Brochmann N,Nielsen K,Thiele J,Jensen MK,Weis Bjerrum O,Kruse TA,Hasselbalch HC

EDAG positively regulates erythroid differentiation and modifies GATA1 acetylation through recruiting p300.
Stem cells (Dayton, Ohio)32(8)2278-89(2014 Aug)
Zheng WW,Dong XM,Yin RH,Xu FF,Ning HM,Zhang MJ,Xu CW,Yang Y,Ding YL,Wang ZD,Zhao WB,Tang LJ,Chen H,Wang XH,Zhan YQ,Yu M,Ge CH,Li CY,Yang XM

Erythroid differentiation-associated gene interacts with NPM1 (nucleophosmin/B23) and increases its protein stability, resisting cell apoptosis.
The FEBS journal279(16)2848-62(2012 Aug)
Zhang MJ,Ding YL,Xu CW,Yang Y,Lian WX,Zhan YQ,Li W,Xu WX,Yu M,Ge CH,Ning HM,Li CY,Yang XM

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HEMGN cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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