UFSP2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UFSP2
Entrez Gene ID	55325
Full Name	UFM1 specific peptidase 2
Synonyms	BHD,C4orf20
General protein information	Preferred Names UFM1 specific peptidase 2 Names ufm1-specific protease 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q35.1
Summary	This gene encodes a highly conserved cysteine protease. The protein cleaves two C-terminal residues from ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein. Activation of ubiquitin-fold modifier 1 by the encoded protein exposes a C-terminal glycine residue that allows interaction with other proteins and transfer to its target protein. An allelic variant of this gene has been associated with Beukes hip dysplasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016].
Disorder MIM:	611482
Disorder Html:	?Hip dysplasia, Beukes type, 142669 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_018359.3	NP_060829.2	ufm1-specific protease 2
NM_018359.5	NP_060829.2	ufm1-specific protease 2
NM_018359.4	NP_060829.2	ufm1-specific protease 2

Pathways from BioSystems

Homologies

	UFSP2	XP_001088243.2
Rattus norvegicus (Norway rat)	Ufsp2	NP_001014164.1
Drosophila melanogaster (fruit fly)	CG16979	NP_648779.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011975	XP_320556.4
Homo sapiens (human)	UFSP2	NP_060829.2
Bos taurus (cattle)	UFSP2	NP_001069945.2
Xenopus tropicalis (tropical clawed frog)	ufsp2	NP_001120460.1
Pan troglodytes (chimpanzee)	UFSP2	XP_517560.3
Canis lupus familiaris (dog)	UFSP2	XP_005630034.1
Mus musculus (house mouse)	Ufsp2	NP_619609.1
Gallus gallus (chicken)	UFSP2	NP_001026307.1
Danio rerio (zebrafish)	ufsp2	NP_942105.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of a mutation in the ubiquitin-fold modifier 1-specific peptidase 2 gene, UFSP2, in an extended South African family with Beukes hip dysplasia.
Watson CM, Crinnion LA, Gleghorn L, Newman WG, Ramesar R, Beighton P, Wallis GA
South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde105(7)558-63(2015 Sep)

Modification of ASC1 by UFM1 is crucial for ER? transactivation and breast cancer development.
Yoo HM, Kang SH, Kim JY, Lee JE, Seong MW, Lee SW, Ka SH, Sou YS, Komatsu M, Tanaka K, Lee ST, Noh DY, Baek SH, Jeon YJ, Chung CH
Molecular cell56(2)261-74(2014 Oct)

Two novel ubiquitin-fold modifier 1 (Ufm1)-specific proteases, UfSP1 and UfSP2.
Kang SH, Kim GR, Seong M, Baek SH, Seol JH, Bang OS, Ovaa H, Tatsumi K, Komatsu M, Tanaka K, Chung CH
The Journal of biological chemistry282(8)5256-62(2007 Feb)

Screening the proteins that interact with calpain in a human heart cDNA library using a yeast two-hybrid system.
Jiang LQ, Wen SJ, Wang HY, Chen LY
Hypertension research : official journal of the Japanese Society of Hypertension25(4)647-52(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following UFSP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UFSP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01932	NM_018359.5 Latest version!	Homo sapiens UFM1 specific peptidase 2 (UFSP2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu01932	NM_018359.3	Homo sapiens UFM1 specific peptidase 2 (UFSP2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu01932	NM_018359.4	Homo sapiens UFM1 specific peptidase 2 (UFSP2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UFSP2 ( NM_018359.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060829.2 Homo sapiens UFM1 specific peptidase 2 (UFSP2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01932
Clone ID Related Accession (Same CDS sequence)	NM_018359.3 , NM_018359.5 , NM_018359.4
Accession Version	NM_018359.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	ufm1-specific protease 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106897.5, DA537391.1, AK002062.1, BC010493.1, BF735169.1, CA432183.1 and AK091635.1. This sequence is a reference standard in the RefSeqGene project. On Jul 21, 2009 this sequence version replaced NM_018359.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.60269.1, SRR1803612.70073.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGATTT CAGAAAGTAT GGATATACTC TTCAGAATAA GAGGAGGCCT TGATTTGGCT 
TTTCAGCTAG CTACTCCTAA TGAAATTTTT CTCAAGAAGG CACTGAAACA TGTGTTGAGT 
GACCTGTCAA CTAAGCTGTC TTCAAACGCC CTTGTGTTCA GAATTTGCCA CAGTTCAGTG 
TATATATGGC CTAGCAGTGA CATAAACACC ATTCCTGGAG AACTGACTGA TGCTTCTGCT 
TGTAAGAACA TACTGCGCTT TATTCAATTT GAGCCAGAAG AAGATATAAA AAGAAAATTC 
ATGAGAAAGA AGGACAAAAA GTTATCAGAC ATGCATCAAA TAGTAAATAT AGATCTTATG 
CTGGAAATGT CAACCTCCCT GGCAGCTGTA ACGCCCATCA TTGAAAGGGA AAGCGGAGGA 
CACCATTATG TTAATATGAC TTTACCTGTC GATGCAGTTA TATCTGTTGC TCCAGAAGAA 
ACATGGGGAA AAGTTCGTAA ACTCCTGGTT GATGCAATTC ATAATCAACT AACTGACATG 
GAAAAATGTA TTTTGAAATA TATGAAAGGA ACATCTATTG TGGTCCCTGA ACCACTGCAC 
TTTTTATTAC CAGGGAAAAA AAATCTTGTA ACAATTTCAT ATCCTTCAGG AATACCAGAT 
GGCCAGCTGC AGGCCTATAG GAAGGAGTTA CATGATCTTT TCAATCTGCC TCACGACAGA 
CCCTATTTCA AAAGGTCTAA TGCTTATCAC TTTCCAGATG AGCCATACAA AGATGGTTAC 
ATTAGAAATC CACATACTTA CCTTAATCCA CCTAACATGG AGACTGGTAT GATTTATGTG 
GTCCAGGGCA TATATGGCTA TCATCATTAT ATGCAGGATC GCATAGATGA CAATGGCTGG 
GGCTGTGCTT ATCGATCTCT GCAGACTATC TGCTCTTGGT TCAAACATCA GGGATACACA 
GAGAGGTCCA TTCCAACACA CAGAGAAATT CAGCAGGCTC TAGTCGATGC CGGGGACAAA 
CCAGCAACAT TTGTCGGATC GCGGCAATGG ATTGGATCTA TTGAGGTGCA GCTGGTACTA 
AACCAATTGA TCGGTATAAC GTCAAAAATC CTGTTTGTCA GCCAAGGTTC AGAAATTGCC 
TCTCAAGGAC GGGAACTGGC TAATCATTTC CAAAGTGAAG GAACTCCAGT TATGATCGGG 
GGAGGAGTTT TGGCCCACAC AATACTAGGA GTTGCATGGA ATGAGATTAC AGGGCAGATA 
AAGTTTCTGA TTCTAGATCC ACATTATACC GGTGCTGAAG ACCTGCAAGT TATTTTGGAA 
AAGGGCTGGT GCGGATGGAA GGGCCCAGAT TTTTGGAACA AGGATGCATA CTATAACTTA 
TGTCTTCCTC AGCGACCAAA TATGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060829.2
CDS	118..1527
Translation	MVISESMDILFRIRGGLDLAFQLATPNEIFLKKALKHVLSDLSTKLSSNALVFRICHSSVYIWPSSDINTIPGELTDASACKNILRFIQFEPEEDIKRKFMRKKDKKLSDMHQIVNIDLMLEMSTSLAAVTPIIERESGGHHYVNMTLPVDAVISVAPEETWGKVRKLLVDAIHNQLTDMEKCILKYMKGTSIVVPEPLHFLLPGKKNLVTISYPSGIPDGQLQAYRKELHDLFNLPHDRPYFKRSNAYHFPDEPYKDGYIRNPHTYLNPPNMETGMIYVVQGIYGYHHYMQDRIDDNGWGCAYRSLQTICSWFKHQGYTERSIPTHREIQQALVDAGDKPATFVGSRQWIGSIEVQLVLNQLIGITSKILFVSQGSEIASQGRELANHFQSEGTPVMIGGGVLAHTILGVAWNEITGQIKFLILDPHYTGAEDLQVILEKGWCGWKGPDFWNKDAYYNLCLPQRPNMI

Target ORF information:

RefSeq Version	NM_018359.3
Organism	Homo sapiens(human)
Definition	Homo sapiens UFM1 specific peptidase 2 (UFSP2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018359.3

ORF Insert Sequence:

ATGGTGATTT CAGAAAGTAT GGATATACTC TTCAGAATAA GAGGAGGCCT TGATTTGGCT 
TTTCAGCTAG CTACTCCTAA TGAAATTTTT CTCAAGAAGG CACTGAAACA TGTGTTGAGT 
GACCTGTCAA CTAAGCTGTC TTCAAACGCC CTTGTGTTCA GAATTTGCCA CAGTTCAGTG 
TATATATGGC CTAGCAGTGA CATAAACACC ATTCCTGGAG AACTGACTGA TGCTTCTGCT 
TGTAAGAACA TACTGCGCTT TATTCAATTT GAGCCAGAAG AAGATATAAA AAGAAAATTC 
ATGAGAAAGA AGGACAAAAA GTTATCAGAC ATGCATCAAA TAGTAAATAT AGATCTTATG 
CTGGAAATGT CAACCTCCCT GGCAGCTGTA ACGCCCATCA TTGAAAGGGA AAGCGGAGGA 
CACCATTATG TTAATATGAC TTTACCTGTC GATGCAGTTA TATCTGTTGC TCCAGAAGAA 
ACATGGGGAA AAGTTCGTAA ACTCCTGGTT GATGCAATTC ATAATCAACT AACTGACATG 
GAAAAATGTA TTTTGAAATA TATGAAAGGA ACATCTATTG TGGTCCCTGA ACCACTGCAC 
TTTTTATTAC CAGGGAAAAA AAATCTTGTA ACAATTTCAT ATCCTTCAGG AATACCAGAT 
GGCCAGCTGC AGGCCTATAG GAAGGAGTTA CATGATCTTT TCAATCTGCC TCACGACAGA 
CCCTATTTCA AAAGGTCTAA TGCTTATCAC TTTCCAGATG AGCCATACAA AGATGGTTAC 
ATTAGAAATC CACATACTTA CCTTAATCCA CCTAACATGG AGACTGGTAT GATTTATGTG 
GTCCAGGGCA TATATGGCTA TCATCATTAT ATGCAGGATC GCATAGATGA CAATGGCTGG 
GGCTGTGCTT ATCGATCTCT GCAGACTATC TGCTCTTGGT TCAAACATCA GGGATACACA 
GAGAGGTCCA TTCCAACACA CAGAGAAATT CAGCAGGCTC TAGTCGATGC CGGGGACAAA 
CCAGCAACAT TTGTCGGATC GCGGCAATGG ATTGGATCTA TTGAGGTGCA GCTGGTACTA 
AACCAATTGA TCGGTATAAC GTCAAAAATC CTGTTTGTCA GCCAAGGTTC AGAAATTGCC 
TCTCAAGGAC GGGAACTGGC TAATCATTTC CAAAGTGAAG GAACTCCAGT TATGATCGGG 
GGAGGAGTTT TGGCCCACAC AATACTAGGA GTTGCATGGA ATGAGATTAC AGGGCAGATA 
AAGTTTCTGA TTCTAGATCC ACATTATACC GGTGCTGAAG ACCTGCAAGT TATTTTGGAA 
AAGGGCTGGT GCGGATGGAA GGGCCCAGAT TTTTGGAACA AGGATGCATA CTATAACTTA 
TGTCTTCCTC AGCGACCAAA TATGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UFSP2 ( NM_018359.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060829.2 Homo sapiens UFM1 specific peptidase 2 (UFSP2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01932
Clone ID Related Accession (Same CDS sequence)	NM_018359.3 , NM_018359.5 , NM_018359.4
Accession Version	NM_018359.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	ufm1-specific protease 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA537391.1, AK002062.1, BC010493.1, BF735169.1, CA432183.1, AC106897.5 and AK091635.1. On Nov 23, 2018 this sequence version replaced NM_018359.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.70073.1, SRR1803614.157286.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGATTT CAGAAAGTAT GGATATACTC TTCAGAATAA GAGGAGGCCT TGATTTGGCT 
TTTCAGCTAG CTACTCCTAA TGAAATTTTT CTCAAGAAGG CACTGAAACA TGTGTTGAGT 
GACCTGTCAA CTAAGCTGTC TTCAAACGCC CTTGTGTTCA GAATTTGCCA CAGTTCAGTG 
TATATATGGC CTAGCAGTGA CATAAACACC ATTCCTGGAG AACTGACTGA TGCTTCTGCT 
TGTAAGAACA TACTGCGCTT TATTCAATTT GAGCCAGAAG AAGATATAAA AAGAAAATTC 
ATGAGAAAGA AGGACAAAAA GTTATCAGAC ATGCATCAAA TAGTAAATAT AGATCTTATG 
CTGGAAATGT CAACCTCCCT GGCAGCTGTA ACGCCCATCA TTGAAAGGGA AAGCGGAGGA 
CACCATTATG TTAATATGAC TTTACCTGTC GATGCAGTTA TATCTGTTGC TCCAGAAGAA 
ACATGGGGAA AAGTTCGTAA ACTCCTGGTT GATGCAATTC ATAATCAACT AACTGACATG 
GAAAAATGTA TTTTGAAATA TATGAAAGGA ACATCTATTG TGGTCCCTGA ACCACTGCAC 
TTTTTATTAC CAGGGAAAAA AAATCTTGTA ACAATTTCAT ATCCTTCAGG AATACCAGAT 
GGCCAGCTGC AGGCCTATAG GAAGGAGTTA CATGATCTTT TCAATCTGCC TCACGACAGA 
CCCTATTTCA AAAGGTCTAA TGCTTATCAC TTTCCAGATG AGCCATACAA AGATGGTTAC 
ATTAGAAATC CACATACTTA CCTTAATCCA CCTAACATGG AGACTGGTAT GATTTATGTG 
GTCCAGGGCA TATATGGCTA TCATCATTAT ATGCAGGATC GCATAGATGA CAATGGCTGG 
GGCTGTGCTT ATCGATCTCT GCAGACTATC TGCTCTTGGT TCAAACATCA GGGATACACA 
GAGAGGTCCA TTCCAACACA CAGAGAAATT CAGCAGGCTC TAGTCGATGC CGGGGACAAA 
CCAGCAACAT TTGTCGGATC GCGGCAATGG ATTGGATCTA TTGAGGTGCA GCTGGTACTA 
AACCAATTGA TCGGTATAAC GTCAAAAATC CTGTTTGTCA GCCAAGGTTC AGAAATTGCC 
TCTCAAGGAC GGGAACTGGC TAATCATTTC CAAAGTGAAG GAACTCCAGT TATGATCGGG 
GGAGGAGTTT TGGCCCACAC AATACTAGGA GTTGCATGGA ATGAGATTAC AGGGCAGATA 
AAGTTTCTGA TTCTAGATCC ACATTATACC GGTGCTGAAG ACCTGCAAGT TATTTTGGAA 
AAGGGCTGGT GCGGATGGAA GGGCCCAGAT TTTTGGAACA AGGATGCATA CTATAACTTA 
TGTCTTCCTC AGCGACCAAA TATGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060829.2
CDS	97..1506
Translation	MVISESMDILFRIRGGLDLAFQLATPNEIFLKKALKHVLSDLSTKLSSNALVFRICHSSVYIWPSSDINTIPGELTDASACKNILRFIQFEPEEDIKRKFMRKKDKKLSDMHQIVNIDLMLEMSTSLAAVTPIIERESGGHHYVNMTLPVDAVISVAPEETWGKVRKLLVDAIHNQLTDMEKCILKYMKGTSIVVPEPLHFLLPGKKNLVTISYPSGIPDGQLQAYRKELHDLFNLPHDRPYFKRSNAYHFPDEPYKDGYIRNPHTYLNPPNMETGMIYVVQGIYGYHHYMQDRIDDNGWGCAYRSLQTICSWFKHQGYTERSIPTHREIQQALVDAGDKPATFVGSRQWIGSIEVQLVLNQLIGITSKILFVSQGSEIASQGRELANHFQSEGTPVMIGGGVLAHTILGVAWNEITGQIKFLILDPHYTGAEDLQVILEKGWCGWKGPDFWNKDAYYNLCLPQRPNMI

Target ORF information:

RefSeq Version	NM_018359.4
Organism	Homo sapiens(human)
Definition	Homo sapiens UFM1 specific peptidase 2 (UFSP2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018359.4

ORF Insert Sequence:

ATGGTGATTT CAGAAAGTAT GGATATACTC TTCAGAATAA GAGGAGGCCT TGATTTGGCT 
TTTCAGCTAG CTACTCCTAA TGAAATTTTT CTCAAGAAGG CACTGAAACA TGTGTTGAGT 
GACCTGTCAA CTAAGCTGTC TTCAAACGCC CTTGTGTTCA GAATTTGCCA CAGTTCAGTG 
TATATATGGC CTAGCAGTGA CATAAACACC ATTCCTGGAG AACTGACTGA TGCTTCTGCT 
TGTAAGAACA TACTGCGCTT TATTCAATTT GAGCCAGAAG AAGATATAAA AAGAAAATTC 
ATGAGAAAGA AGGACAAAAA GTTATCAGAC ATGCATCAAA TAGTAAATAT AGATCTTATG 
CTGGAAATGT CAACCTCCCT GGCAGCTGTA ACGCCCATCA TTGAAAGGGA AAGCGGAGGA 
CACCATTATG TTAATATGAC TTTACCTGTC GATGCAGTTA TATCTGTTGC TCCAGAAGAA 
ACATGGGGAA AAGTTCGTAA ACTCCTGGTT GATGCAATTC ATAATCAACT AACTGACATG 
GAAAAATGTA TTTTGAAATA TATGAAAGGA ACATCTATTG TGGTCCCTGA ACCACTGCAC 
TTTTTATTAC CAGGGAAAAA AAATCTTGTA ACAATTTCAT ATCCTTCAGG AATACCAGAT 
GGCCAGCTGC AGGCCTATAG GAAGGAGTTA CATGATCTTT TCAATCTGCC TCACGACAGA 
CCCTATTTCA AAAGGTCTAA TGCTTATCAC TTTCCAGATG AGCCATACAA AGATGGTTAC 
ATTAGAAATC CACATACTTA CCTTAATCCA CCTAACATGG AGACTGGTAT GATTTATGTG 
GTCCAGGGCA TATATGGCTA TCATCATTAT ATGCAGGATC GCATAGATGA CAATGGCTGG 
GGCTGTGCTT ATCGATCTCT GCAGACTATC TGCTCTTGGT TCAAACATCA GGGATACACA 
GAGAGGTCCA TTCCAACACA CAGAGAAATT CAGCAGGCTC TAGTCGATGC CGGGGACAAA 
CCAGCAACAT TTGTCGGATC GCGGCAATGG ATTGGATCTA TTGAGGTGCA GCTGGTACTA 
AACCAATTGA TCGGTATAAC GTCAAAAATC CTGTTTGTCA GCCAAGGTTC AGAAATTGCC 
TCTCAAGGAC GGGAACTGGC TAATCATTTC CAAAGTGAAG GAACTCCAGT TATGATCGGG 
GGAGGAGTTT TGGCCCACAC AATACTAGGA GTTGCATGGA ATGAGATTAC AGGGCAGATA 
AAGTTTCTGA TTCTAGATCC ACATTATACC GGTGCTGAAG ACCTGCAAGT TATTTTGGAA 
AAGGGCTGGT GCGGATGGAA GGGCCCAGAT TTTTGGAACA AGGATGCATA CTATAACTTA 
TGTCTTCCTC AGCGACCAAA TATGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UFSP2 ( NM_018359.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060829.2 Homo sapiens UFM1 specific peptidase 2 (UFSP2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01932
Clone ID Related Accession (Same CDS sequence)	NM_018359.3 , NM_018359.5 , NM_018359.4
Accession Version	NM_018359.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	ufm1-specific protease 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106897.5. On Aug 31, 2019 this sequence version replaced NM_018359.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.80002.1, SRR1803612.70073.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264689.11/ ENSP00000264689.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGATTT CAGAAAGTAT GGATATACTC TTCAGAATAA GAGGAGGCCT TGATTTGGCT 
TTTCAGCTAG CTACTCCTAA TGAAATTTTT CTCAAGAAGG CACTGAAACA TGTGTTGAGT 
GACCTGTCAA CTAAGCTGTC TTCAAACGCC CTTGTGTTCA GAATTTGCCA CAGTTCAGTG 
TATATATGGC CTAGCAGTGA CATAAACACC ATTCCTGGAG AACTGACTGA TGCTTCTGCT 
TGTAAGAACA TACTGCGCTT TATTCAATTT GAGCCAGAAG AAGATATAAA AAGAAAATTC 
ATGAGAAAGA AGGACAAAAA GTTATCAGAC ATGCATCAAA TAGTAAATAT AGATCTTATG 
CTGGAAATGT CAACCTCCCT GGCAGCTGTA ACGCCCATCA TTGAAAGGGA AAGCGGAGGA 
CACCATTATG TTAATATGAC TTTACCTGTC GATGCAGTTA TATCTGTTGC TCCAGAAGAA 
ACATGGGGAA AAGTTCGTAA ACTCCTGGTT GATGCAATTC ATAATCAACT AACTGACATG 
GAAAAATGTA TTTTGAAATA TATGAAAGGA ACATCTATTG TGGTCCCTGA ACCACTGCAC 
TTTTTATTAC CAGGGAAAAA AAATCTTGTA ACAATTTCAT ATCCTTCAGG AATACCAGAT 
GGCCAGCTGC AGGCCTATAG GAAGGAGTTA CATGATCTTT TCAATCTGCC TCACGACAGA 
CCCTATTTCA AAAGGTCTAA TGCTTATCAC TTTCCAGATG AGCCATACAA AGATGGTTAC 
ATTAGAAATC CACATACTTA CCTTAATCCA CCTAACATGG AGACTGGTAT GATTTATGTG 
GTCCAGGGCA TATATGGCTA TCATCATTAT ATGCAGGATC GCATAGATGA CAATGGCTGG 
GGCTGTGCTT ATCGATCTCT GCAGACTATC TGCTCTTGGT TCAAACATCA GGGATACACA 
GAGAGGTCCA TTCCAACACA CAGAGAAATT CAGCAGGCTC TAGTCGATGC CGGGGACAAA 
CCAGCAACAT TTGTCGGATC GCGGCAATGG ATTGGATCTA TTGAGGTGCA GCTGGTACTA 
AACCAATTGA TCGGTATAAC GTCAAAAATC CTGTTTGTCA GCCAAGGTTC AGAAATTGCC 
TCTCAAGGAC GGGAACTGGC TAATCATTTC CAAAGTGAAG GAACTCCAGT TATGATCGGG 
GGAGGAGTTT TGGCCCACAC AATACTAGGA GTTGCATGGA ATGAGATTAC AGGGCAGATA 
AAGTTTCTGA TTCTAGATCC ACATTATACC GGTGCTGAAG ACCTGCAAGT TATTTTGGAA 
AAGGGCTGGT GCGGATGGAA GGGCCCAGAT TTTTGGAACA AGGATGCATA CTATAACTTA 
TGTCTTCCTC AGCGACCAAA TATGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060829.2
CDS	97..1506
Translation	MVISESMDILFRIRGGLDLAFQLATPNEIFLKKALKHVLSDLSTKLSSNALVFRICHSSVYIWPSSDINTIPGELTDASACKNILRFIQFEPEEDIKRKFMRKKDKKLSDMHQIVNIDLMLEMSTSLAAVTPIIERESGGHHYVNMTLPVDAVISVAPEETWGKVRKLLVDAIHNQLTDMEKCILKYMKGTSIVVPEPLHFLLPGKKNLVTISYPSGIPDGQLQAYRKELHDLFNLPHDRPYFKRSNAYHFPDEPYKDGYIRNPHTYLNPPNMETGMIYVVQGIYGYHHYMQDRIDDNGWGCAYRSLQTICSWFKHQGYTERSIPTHREIQQALVDAGDKPATFVGSRQWIGSIEVQLVLNQLIGITSKILFVSQGSEIASQGRELANHFQSEGTPVMIGGGVLAHTILGVAWNEITGQIKFLILDPHYTGAEDLQVILEKGWCGWKGPDFWNKDAYYNLCLPQRPNMI

Target ORF information:

RefSeq Version	NM_018359.5
Organism	Homo sapiens(human)
Definition	Homo sapiens UFM1 specific peptidase 2 (UFSP2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018359.5

ORF Insert Sequence:

ATGGTGATTT CAGAAAGTAT GGATATACTC TTCAGAATAA GAGGAGGCCT TGATTTGGCT 
TTTCAGCTAG CTACTCCTAA TGAAATTTTT CTCAAGAAGG CACTGAAACA TGTGTTGAGT 
GACCTGTCAA CTAAGCTGTC TTCAAACGCC CTTGTGTTCA GAATTTGCCA CAGTTCAGTG 
TATATATGGC CTAGCAGTGA CATAAACACC ATTCCTGGAG AACTGACTGA TGCTTCTGCT 
TGTAAGAACA TACTGCGCTT TATTCAATTT GAGCCAGAAG AAGATATAAA AAGAAAATTC 
ATGAGAAAGA AGGACAAAAA GTTATCAGAC ATGCATCAAA TAGTAAATAT AGATCTTATG 
CTGGAAATGT CAACCTCCCT GGCAGCTGTA ACGCCCATCA TTGAAAGGGA AAGCGGAGGA 
CACCATTATG TTAATATGAC TTTACCTGTC GATGCAGTTA TATCTGTTGC TCCAGAAGAA 
ACATGGGGAA AAGTTCGTAA ACTCCTGGTT GATGCAATTC ATAATCAACT AACTGACATG 
GAAAAATGTA TTTTGAAATA TATGAAAGGA ACATCTATTG TGGTCCCTGA ACCACTGCAC 
TTTTTATTAC CAGGGAAAAA AAATCTTGTA ACAATTTCAT ATCCTTCAGG AATACCAGAT 
GGCCAGCTGC AGGCCTATAG GAAGGAGTTA CATGATCTTT TCAATCTGCC TCACGACAGA 
CCCTATTTCA AAAGGTCTAA TGCTTATCAC TTTCCAGATG AGCCATACAA AGATGGTTAC 
ATTAGAAATC CACATACTTA CCTTAATCCA CCTAACATGG AGACTGGTAT GATTTATGTG 
GTCCAGGGCA TATATGGCTA TCATCATTAT ATGCAGGATC GCATAGATGA CAATGGCTGG 
GGCTGTGCTT ATCGATCTCT GCAGACTATC TGCTCTTGGT TCAAACATCA GGGATACACA 
GAGAGGTCCA TTCCAACACA CAGAGAAATT CAGCAGGCTC TAGTCGATGC CGGGGACAAA 
CCAGCAACAT TTGTCGGATC GCGGCAATGG ATTGGATCTA TTGAGGTGCA GCTGGTACTA 
AACCAATTGA TCGGTATAAC GTCAAAAATC CTGTTTGTCA GCCAAGGTTC AGAAATTGCC 
TCTCAAGGAC GGGAACTGGC TAATCATTTC CAAAGTGAAG GAACTCCAGT TATGATCGGG 
GGAGGAGTTT TGGCCCACAC AATACTAGGA GTTGCATGGA ATGAGATTAC AGGGCAGATA 
AAGTTTCTGA TTCTAGATCC ACATTATACC GGTGCTGAAG ACCTGCAAGT TATTTTGGAA 
AAGGGCTGGT GCGGATGGAA GGGCCCAGAT TTTTGGAACA AGGATGCATA CTATAACTTA 
TGTCTTCCTC AGCGACCAAA TATGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of a mutation in the ubiquitin-fold modifier 1-specific peptidase 2 gene, UFSP2, in an extended South African family with Beukes hip dysplasia.
South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde105(7)558-63(2015 Sep)
Watson CM,Crinnion LA,Gleghorn L,Newman WG,Ramesar R,Beighton P,Wallis GA

Modification of ASC1 by UFM1 is crucial for ER? transactivation and breast cancer development.
Molecular cell56(2)261-74(2014 Oct)
Yoo HM,Kang SH,Kim JY,Lee JE,Seong MW,Lee SW,Ka SH,Sou YS,Komatsu M,Tanaka K,Lee ST,Noh DY,Baek SH,Jeon YJ,Chung CH

Two novel ubiquitin-fold modifier 1 (Ufm1)-specific proteases, UfSP1 and UfSP2.
The Journal of biological chemistry282(8)5256-62(2007 Feb)
Kang SH,Kim GR,Seong M,Baek SH,Seol JH,Bang OS,Ovaa H,Tatsumi K,Komatsu M,Tanaka K,Chung CH

Screening the proteins that interact with calpain in a human heart cDNA library using a yeast two-hybrid system.
Hypertension research : official journal of the Japanese Society of Hypertension25(4)647-52(2002 Jul)
Jiang LQ,Wen SJ,Wang HY,Chen LY

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UFSP2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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