ADI1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADI1
Entrez Gene ID	55256
Full Name	acireductone dioxygenase 1
Synonyms	APL1,ARD,Fe-ARD,HMFT1638,MTCBP1,Ni-ARD,SIPL,mtnD
General protein information	Preferred Names acireductone dioxygenase 1 Names 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase MT1-MMP cytoplasmic tail-binding protein-1 acireductone dioxygenase (Fe(2+)-requiring) acireductone dioxygenase (Ni(2+)-requiring) membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1 submergence induced protein 2 submergence-induced protein-like factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p25.3
Summary	This gene encodes an enzyme that belongs to the aci-reductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Related pseudogenes have been defined on chromosomes 8 and 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015].
Disorder MIM:	613400

mRNA and Protein(s)

mRNA	Protein	Name
NM_001306077.1	NP_001293006.1	1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase isoform 2
NM_018269.3	NP_060739.2	1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase isoform 1
NM_018269.4	NP_060739.2	1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase isoform 1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	ARD1	NP_567443.1
Oryza sativa (rice)	Os10g0419400	NP_001064618.1
Homo sapiens (human)	ADI1	NP_060739.2
Canis lupus familiaris (dog)	ADI1	XP_005630647.1
Bos taurus (cattle)	ADI1	NP_001030545.1
Mus musculus (house mouse)	Adi1	NP_598813.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005618	XP_315627.3
Canis lupus familiaris (dog)	LOC100856577	XP_003640360.1
Rattus norvegicus (Norway rat)	Adi1	NP_954528.1
Danio rerio (zebrafish)	adi1	NP_955962.1
Drosophila melanogaster (fruit fly)	CG32068	NP_001097577.1
Xenopus tropicalis (tropical clawed frog)	adi1	NP_001004933.1
	LOC722050	XP_001118248.1
Gallus gallus (chicken)	ADI1	NP_001026260.1
Pan troglodytes (chimpanzee)	ADI1	XP_001153347.1
Arabidopsis thaliana (thale cress)	ATARD2	NP_001031640.2
Arabidopsis thaliana (thale cress)	ARD3	NP_180208.1
Oryza sativa (rice)	Os03g0161800	NP_001049046.1

Gene Ontology
Bibliography

Related articles in PubMed

Dual chemistry catalyzed by human acireductone dioxygenase.
Deshpande AR, Pochapsky TC, Petsko GA, Ringe D
Protein engineering, design & selection : PEDS30(3)197-204(2017 Mar)

Interaction between hepatic membrane type 1 matrix metalloproteinase and acireductone dioxygenase 1 regulates hepatitis C virus infection.
Chang ML, Huang YH, Cheng JC, Yeh CT
Journal of viral hepatitis23(4)256-66(2016 Apr)

Evidence of MTCBP-1 interaction with the cytoplasmic domain of MT1-MMP: Implications in the autophagy cell index of high-grade glioblastoma.
Pratt J, Iddir M, Bourgault S, Annabi B
Molecular carcinogenesis55(2)148-60(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The thermal stability of the HsARD isozymes is a function of the metal ion identity, with Ni2+-bound HsARD being the most stable followed by Co2+ and Fe2+, and Mn2+-bound HsARD being the least stable.
Title: Dual chemistry catalyzed by human acireductone dioxygenase.

In summary, clinical and cell-based experiments suggested that physical interaction between MT1-MMP and ADI1 led to suppression of hepatitis C virus infection. This inhibitory effect could be reversed by ADI1 overexpression.
Title: Interaction between hepatic membrane type 1 matrix metalloproteinase and acireductone dioxygenase 1 regulates hepatitis C virus infection.

Data elucidate a new role for MTCBP-1 regulating the intracellular function of MT1-MMP-mediated autophagy. Their inverse expression correlation with brain tumor grades could also contribute to the decreased autophagic index observed in high-grade tumors.
Title: Evidence of MTCBP-1 interaction with the cytoplasmic domain of MT1-MMP: Implications in the autophagy cell index of high-grade glioblastoma.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

293-ADI1-CD81 cells are permissive for serum-derived HCV infection.
Title: 293 cells over-expressing human ADI1 and CD81 are permissive for serum-derived hepatitis C virus infection.

The following ADI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02749	NM_018269.4 Latest version!	Homo sapiens acireductone dioxygenase 1 (ADI1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu35204	NM_001306077.1 Latest version!	Homo sapiens acireductone dioxygenase 1 (ADI1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu02749	NM_018269.3	Homo sapiens acireductone dioxygenase 1 (ADI1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADI1 ( NM_018269.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060739.2 Homo sapiens acireductone dioxygenase 1 (ADI1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02749
Clone ID Related Accession (Same CDS sequence)	NM_018269.3 , NM_018269.4
Accession Version	NM_018269.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB129674.1, DA559726.1, BC001467.2 and AB073609.1. On May 13, 2010 this sequence version replaced NM_018269.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AB073609.1, SRR1803615.231221.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467146, SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCAGG CCTGGTATAT GGACGACGCC CCGGGCGACC CGCGGCAACC CCACCGCCCC 
GACCCCGGCC GCCCAGTGGG CCTGGAGCAG CTGCGGCGGC TCGGGGTGCT CTACTGGAAG 
CTGGATGCTG ACAAATATGA GAATGATCCA GAATTAGAAA AGATCCGAAG AGAGAGGAAC 
TACTCCTGGA TGGACATCAT AACCATATGC AAAGATAAAC TACCAAATTA TGAAGAAAAG 
ATTAAGATGT TCTACGAGGA GCATTTGCAC TTGGACGATG AGATCCGCTA CATCCTGGAT 
GGCAGTGGGT ACTTCGATGT GAGGGACAAG GAGGACCAGT GGATCCGGAT CTTCATGGAG 
AAGGGAGACA TGGTGACGCT CCCCGCGGGG ATCTATCACC GCTTCACGGT GGACGAGAAG 
AACTACACGA AGGCCATGCG GCTGTTTGTG GGAGAACCGG TGTGGACAGC GTACAACCGG 
CCCGCTGACC ATTTTGAAGC CCGCGGGCAG TACGTGAAAT TTCTGGCACA GACCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060739.2
CDS	93..632
Translation	MVQAWYMDDAPGDPRQPHRPDPGRPVGLEQLRRLGVLYWKLDADKYENDPELEKIRRERNYSWMDIITICKDKLPNYEEKIKMFYEEHLHLDDEIRYILDGSGYFDVRDKEDQWIRIFMEKGDMVTLPAGIYHRFTVDEKNYTKAMRLFVGEPVWTAYNRPADHFEARGQYVKFLAQTA

Target ORF information:

RefSeq Version	NM_018269.3
Organism	Homo sapiens(human)
Definition	Homo sapiens acireductone dioxygenase 1 (ADI1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018269.3

ORF Insert Sequence:

ATGGTGCAGG CCTGGTATAT GGACGACGCC CCGGGCGACC CGCGGCAACC CCACCGCCCC 
GACCCCGGCC GCCCAGTGGG CCTGGAGCAG CTGCGGCGGC TCGGGGTGCT CTACTGGAAG 
CTGGATGCTG ACAAATATGA GAATGATCCA GAATTAGAAA AGATCCGAAG AGAGAGGAAC 
TACTCCTGGA TGGACATCAT AACCATATGC AAAGATAAAC TACCAAATTA TGAAGAAAAG 
ATTAAGATGT TCTACGAGGA GCATTTGCAC TTGGACGATG AGATCCGCTA CATCCTGGAT 
GGCAGTGGGT ACTTCGATGT GAGGGACAAG GAGGACCAGT GGATCCGGAT CTTCATGGAG 
AAGGGAGACA TGGTGACGCT CCCCGCGGGG ATCTATCACC GCTTCACGGT GGACGAGAAG 
AACTACACGA AGGCCATGCG GCTGTTTGTG GGAGAACCGG TGTGGACAGC GTACAACCGG 
CCCGCTGACC ATTTTGAAGC CCGCGGGCAG TACGTGAAAT TTCTGGCACA GACCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADI1 ( NM_018269.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060739.2 Homo sapiens acireductone dioxygenase 1 (ADI1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02749
Clone ID Related Accession (Same CDS sequence)	NM_018269.3 , NM_018269.4
Accession Version	NM_018269.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB129674.1, BC001467.2, AB073609.1 and AC114810.4. On Nov 22, 2018 this sequence version replaced NM_018269.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.151555.1, SRR1803614.65319.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155590, SAMEA2162328 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000327435.11/ ENSP00000333666.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCAGG CCTGGTATAT GGACGACGCC CCGGGCGACC CGCGGCAACC CCACCGCCCC 
GACCCCGGCC GCCCAGTGGG CCTGGAGCAG CTGCGGCGGC TCGGGGTGCT CTACTGGAAG 
CTGGATGCTG ACAAATATGA GAATGATCCA GAATTAGAAA AGATCCGAAG AGAGAGGAAC 
TACTCCTGGA TGGACATCAT AACCATATGC AAAGATAAAC TACCAAATTA TGAAGAAAAG 
ATTAAGATGT TCTACGAGGA GCATTTGCAC TTGGACGATG AGATCCGCTA CATCCTGGAT 
GGCAGTGGGT ACTTCGATGT GAGGGACAAG GAGGACCAGT GGATCCGGAT CTTCATGGAG 
AAGGGAGACA TGGTGACGCT CCCCGCGGGG ATCTATCACC GCTTCACGGT GGACGAGAAG 
AACTACACGA AGGCCATGCG GCTGTTTGTG GGAGAACCGG TGTGGACAGC GTACAACCGG 
CCCGCTGACC ATTTTGAAGC CCGCGGGCAG TACGTGAAAT TTCTGGCACA GACCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060739.2
CDS	45..584
Translation	MVQAWYMDDAPGDPRQPHRPDPGRPVGLEQLRRLGVLYWKLDADKYENDPELEKIRRERNYSWMDIITICKDKLPNYEEKIKMFYEEHLHLDDEIRYILDGSGYFDVRDKEDQWIRIFMEKGDMVTLPAGIYHRFTVDEKNYTKAMRLFVGEPVWTAYNRPADHFEARGQYVKFLAQTA

Target ORF information:

RefSeq Version	NM_018269.4
Organism	Homo sapiens(human)
Definition	Homo sapiens acireductone dioxygenase 1 (ADI1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018269.4

ORF Insert Sequence:

ATGGTGCAGG CCTGGTATAT GGACGACGCC CCGGGCGACC CGCGGCAACC CCACCGCCCC 
GACCCCGGCC GCCCAGTGGG CCTGGAGCAG CTGCGGCGGC TCGGGGTGCT CTACTGGAAG 
CTGGATGCTG ACAAATATGA GAATGATCCA GAATTAGAAA AGATCCGAAG AGAGAGGAAC 
TACTCCTGGA TGGACATCAT AACCATATGC AAAGATAAAC TACCAAATTA TGAAGAAAAG 
ATTAAGATGT TCTACGAGGA GCATTTGCAC TTGGACGATG AGATCCGCTA CATCCTGGAT 
GGCAGTGGGT ACTTCGATGT GAGGGACAAG GAGGACCAGT GGATCCGGAT CTTCATGGAG 
AAGGGAGACA TGGTGACGCT CCCCGCGGGG ATCTATCACC GCTTCACGGT GGACGAGAAG 
AACTACACGA AGGCCATGCG GCTGTTTGTG GGAGAACCGG TGTGGACAGC GTACAACCGG 
CCCGCTGACC ATTTTGAAGC CCGCGGGCAG TACGTGAAAT TTCTGGCACA GACCGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADI1 ( NM_001306077.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001293006.1 Homo sapiens acireductone dioxygenase 1 (ADI1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu35204
Clone ID Related Accession (Same CDS sequence)	NM_001306077.1
Accession Version	NM_001306077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK127473.1, AC231981.2 and AB073609.1. On Apr 14, 2015 this sequence version replaced XM_005264696.2. Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK127473.1, SRR1803615.63373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144835, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGTGA AATCTTCAAA ACTTATGTTC CTCTGCCATG GCTCCAGCCG GTCCCTCCAT 
TCGGGGTCCC TGACTTCCTG CAACACTGGA GTTTGTTGTT CACTGGATGC TGACAAATAT 
GAGAATGATC CAGAATTAGA AAAGATCCGA AGAGAGAGGA ACTACTCCTG GATGGACATC 
ATAACCATAT GCAAAGATAA ACTACCAAAT TATGAAGAAA AGATTAAGAT GTTCTACGAG 
GAGCATTTGC ACTTGGACGA TGAGATCCGC TACATCCTGG ATGGCAGTGG GTACTTCGAT 
GTGAGGGACA AGGAGGACCA GTGGATCCGG ATCTTCATGG AGAAGGGAGA CATGGTGACG 
CTCCCCGCGG GGATCTATCA CCGCTTCACG GTGGACGAGA AGAACTACAC GAAGGCCATG 
CGGCTGTTTG TGGGAGAACC GGTGTGGACA GCGTACAACC GGCCCGCTGA CCATTTTGAA 
GCCCGCGGGC AGTACGTGAA ATTTCTGGCA CAGACCGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293006.1
CDS	2839..3360
Translation	MSVKSSKLMFLCHGSSRSLHSGSLTSCNTGVCCSLDADKYENDPELEKIRRERNYSWMDIITICKDKLPNYEEKIKMFYEEHLHLDDEIRYILDGSGYFDVRDKEDQWIRIFMEKGDMVTLPAGIYHRFTVDEKNYTKAMRLFVGEPVWTAYNRPADHFEARGQYVKFLAQTA

Target ORF information:

RefSeq Version	NM_001306077.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acireductone dioxygenase 1 (ADI1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306077.1

ORF Insert Sequence:

ATGTCCGTGA AATCTTCAAA ACTTATGTTC CTCTGCCATG GCTCCAGCCG GTCCCTCCAT 
TCGGGGTCCC TGACTTCCTG CAACACTGGA GTTTGTTGTT CACTGGATGC TGACAAATAT 
GAGAATGATC CAGAATTAGA AAAGATCCGA AGAGAGAGGA ACTACTCCTG GATGGACATC 
ATAACCATAT GCAAAGATAA ACTACCAAAT TATGAAGAAA AGATTAAGAT GTTCTACGAG 
GAGCATTTGC ACTTGGACGA TGAGATCCGC TACATCCTGG ATGGCAGTGG GTACTTCGAT 
GTGAGGGACA AGGAGGACCA GTGGATCCGG ATCTTCATGG AGAAGGGAGA CATGGTGACG 
CTCCCCGCGG GGATCTATCA CCGCTTCACG GTGGACGAGA AGAACTACAC GAAGGCCATG 
CGGCTGTTTG TGGGAGAACC GGTGTGGACA GCGTACAACC GGCCCGCTGA CCATTTTGAA 
GCCCGCGGGC AGTACGTGAA ATTTCTGGCA CAGACCGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dual chemistry catalyzed by human acireductone dioxygenase.
Protein engineering, design & selection : PEDS30(3)197-204(2017 Mar)
Deshpande AR,Pochapsky TC,Petsko GA,Ringe D

Interaction between hepatic membrane type 1 matrix metalloproteinase and acireductone dioxygenase 1 regulates hepatitis C virus infection.
Journal of viral hepatitis23(4)256-66(2016 Apr)
Chang ML,Huang YH,Cheng JC,Yeh CT

Evidence of MTCBP-1 interaction with the cytoplasmic domain of MT1-MMP: Implications in the autophagy cell index of high-grade glioblastoma.
Molecular carcinogenesis55(2)148-60(2016 Feb)
Pratt J,Iddir M,Bourgault S,Annabi B

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ADI1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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