P3H2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	P3H2
Entrez Gene ID	55214
Full Name	prolyl 3-hydroxylase 2
Synonyms	LEPREL1,MCVD,MLAT4
General protein information	Preferred Names prolyl 3-hydroxylase 2 Names prolyl 3-hydroxylase 2 leprecan-like 1 myxoid liposarcoma-associated protein 4 procollagen-proline 3-dioxygenase 2 prolyl 3-hydroxylase 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q28
Summary	This gene encodes a member of the prolyl 3-hydroxylase subfamily of 2-oxo-glutarate-dependent dioxygenases. These enzymes play a critical role in collagen chain assembly, stability and cross-linking by catalyzing post-translational 3-hydroxylation of proline residues. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011].
Disorder MIM:	610341
Disorder Html:	Myopia, high, with cataract and vitreoretinal degeneration, 614292 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001134418.1	NP_001127890.1	prolyl 3-hydroxylase 2 isoform b
NM_018192.4	NP_060662.2	prolyl 3-hydroxylase 2 isoform a precursor
XM_011512955.1	XP_011511257.1	prolyl 3-hydroxylase 2 isoform X1
NM_018192.3	NP_060662.2	prolyl 3-hydroxylase 2 isoform a precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC566697	XP_003201113.1
Xenopus tropicalis (tropical clawed frog)	leprel1	XP_002934088.1
Homo sapiens (human)	LEPREL1	NP_060662.2
Canis lupus familiaris (dog)	LEPREL1	XP_535843.2
Mus musculus (house mouse)	Leprel1	NP_775555.1
Pan troglodytes (chimpanzee)	LEPREL1	XP_001160886.1
Bos taurus (cattle)	LEPREL1	NP_001093815.1
Rattus norvegicus (Norway rat)	Leprel1	NP_001020798.1
Gallus gallus (chicken)	LEPREL1	NP_001001530.1
	LEPREL1	XP_001092797.1

Gene Ontology
Bibliography

Related articles in PubMed

Recessive mutations in LEPREL1 underlie a recognizable lens subluxation phenotype.
Khan AO, Aldahmesh MA, Alsharif H, Alkuraya FS
Ophthalmic genetics36(1)58-63(2015 Mar)

Homozygous loss-of-function mutation of the LEPREL1 gene causes severe non-syndromic high myopia with early-onset cataract.
Guo H, Tong P, Peng Y, Wang T, Liu Y, Chen J, Li Y, Tian Q, Hu Y, Zheng Y, Xiao L, Xiong W, Pan Q, Hu Z, Xia K
Clinical genetics86(6)575-9(2014 Dec)

Detection of mutations in LRPAP1, CTSH, LEPREL1, ZNF644, SLC39A5, and SCO2 in 298 families with early-onset high myopia by exome sequencing.
Jiang D, Li J, Xiao X, Li S, Jia X, Sun W, Guo X, Zhang Q
Investigative ophthalmology & visual science56(1)339-45(2014 Dec)

High myopia caused by a mutation in LEPREL1, encoding prolyl 3-hydroxylase 2.
Mordechai S, Gradstein L, Pasanen A, Ofir R, El Amour K, Levy J, Belfair N, Lifshitz T, Joshua S, Narkis G, Elbedour K, Myllyharju J, Birk OS
American journal of human genetics89(3)438-45(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Recessive LEPREL1 mutations should be recognized as part of the differential diagnosis of lens subluxation.
Title: Recessive mutations in LEPREL1 underlie a recognizable lens subluxation phenotype.

LEPREL1 plays an important role in eye development and homozygous loss-of-function mutation of this gene can cause severely high myopia and early-onset cataract.
Title: Homozygous loss-of-function mutation of the LEPREL1 gene causes severe non-syndromic high myopia with early-onset cataract.

mutation in LRPAP1 is associated with high myopia. Further studies are expected to evaluate the pathogenicity of the variants in CTSH, LEPREL1, ZNF644, SLC39A5, and SCO2.
Title: Detection of mutations in LRPAP1, CTSH, LEPREL1, ZNF644, SLC39A5, and SCO2 in 298 families with early-onset high myopia by exome sequencing.

P3H2 has preferred substrate sequences among the classes of 3Hyp sites in clade A collagen chains
Title: A role for prolyl 3-hydroxylase 2 in post-translational modification of fibril-forming collagens.

High myopia is caused by a mutation in LEPREL1, encoding prolyl 3-hydroxylase 2.
Title: High myopia caused by a mutation in LEPREL1, encoding prolyl 3-hydroxylase 2.

The following P3H2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P3H2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19470	NM_001134418.1 Latest version!	Homo sapiens prolyl 3-hydroxylase 2 (P3H2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu19470	XM_011512955.1 Latest version!	Homo sapiens prolyl 3-hydroxylase 2 (P3H2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu02725	NM_018192.4 Latest version!	Homo sapiens prolyl 3-hydroxylase 2 (P3H2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$496.30 $709.00
View Old Accession Versions >>
OHu02725	NM_018192.3	Homo sapiens prolyl 3-hydroxylase 2 (P3H2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$496.30 $709.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P3H2 ( NM_001134418.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001127890.1 Homo sapiens prolyl 3-hydroxylase 2 (P3H2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu19470
Clone ID Related Accession (Same CDS sequence)	NM_001134418.1 , XM_011512955.1
Accession Version	NM_001134418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	prolyl 3-hydroxylase 2 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA720646.1, BC005029.1, AC016966.19, BU621129.1 and BU737886.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005029.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAATGC AGCAGAACAT TGAGAATTAC AGGGCGACAG CTGGTGTTGA AGCATTGCAG 
TTGGTAGACA GAGAAGCCAA GCCACACATG GAGAGTTACA ATGCAGGAGT TAAACATTAT 
GAGGCTGATG ACTTTGAGAT GGCTATCAGG CACTTCGAAC AAGCCTTAAG AGAATATTTC 
GTTGAAGATA CAGAATGCCG GACCCTATGT GAGGGGCCTC AGAGATTTGA AGAATATGAG 
TATTTAGGGT ATAAGGCTGG TCTGTATGAA GCTATTGCAG ATCACTACAT GCAGGTGCTT 
GTTTGTCAGC ATGAATGTGT GAGGGAACTT GCCACCCGCC CTGGCCGCCT CTCTCCCATC 
GAGAATTTTC TTCCTCTGCA CTATGATTAC CTACAGTTTG CCTACTATCG AGTTGGTGAG 
TATGTGAAAG CCCTGGAGTG TGCCAAAGCC TATCTTCTAT GCCATCCAGA TGATGAGGAT 
GTCCTAGACA ATGTGGATTA CTATGAGAGT CTGCTGGATG ATAGCATTGA CCCGGCATCC 
ATTGAGGCCA GAGAGGATTT AACAATGTTT GTGAAACGTC ATAAGCTGGA GTCTGAGCTG 
ATAAAATCAG CTGCAGAAGG TCTGGGGTTT TCATACACTG AACCGAATTA TTGGATCAGA 
TATGGAGGAC GACAGGATGA GAATCGGGTC CCTTCAGGAG TGAACGTAGA GGGAGCAGAA 
GTTCATGGAT TCTCAATGGG AAAAAAGCTA TCACCCAAGA TAGATCGAGA CCTAAGAGAA 
GGTGGTCCTC TACTCTATGA GAACATCACA TTCGTCTACA ACTCGGAGCA GCTGAACGGG 
ACTCAGCGGG TTCTCCTGGA TAACGTCCTG TCGGAAGAAC AGTGCCGGGA GCTCCACAGC 
GTGGCCAGTG GAATCATGCT TGTTGGTGAT GGATACAGAG GAAAAACTTC ACCCCATACA 
CCCAATGAAA AGTTTGAAGG TGCAACTGTC CTGAAAGCAC TCAAATCTGG TTATGAAGGT 
CGAGTCCCAC TGAAGAGCGC TCGTCTGTTT TATGACATCA GCGAAAAGGC TCGAAGGATT 
GTAGAATCTT ATTTTATGCT GAACTCAACT CTGTATTTTT CCTATACACA CATGGTCTGC 
CGAACAGCCC TGTCTGGTCA GCAGGATAGA AGAAATGACC TCAGTCATCC CATCCATGCT 
GACAACTGTT TGTTGGATCC AGAGGCCAAC GAATGCTGGA AGGAGCCTCC TGCTTACACA 
TTTCGAGACT ATAGTGCTCT CCTATATATG AATGATGACT TTGAAGGAGG AGAATTCATA 
TTCACAGAGA TGGATGCTAA GACTGTGACT GCCTCTATAA AACCAAAATG TGGGCGCATG 
ATCAGCTTCT CATCTGGAGG AGAGAACCCT CATGGGGTGA AGGCAGTCAC CAAGGGAAAG 
AGGTGTGCTG TGGCTCTGTG GTTCACCTTG GACCCACTTT ATAGAGAATT GGAGCGAATA 
CAGGCTGATG AAGTGATTGC AATTCTGGAT CAAGAACAGC AAGGGAAGCA TGAACTGAAT 
ATCAACCCTA AAGATGAGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127890.1
CDS	299..1882
Translation	MEMQQNIENYRATAGVEALQLVDREAKPHMESYNAGVKHYEADDFEMAIRHFEQALREYFVEDTECRTLCEGPQRFEEYEYLGYKAGLYEAIADHYMQVLVCQHECVRELATRPGRLSPIENFLPLHYDYLQFAYYRVGEYVKALECAKAYLLCHPDDEDVLDNVDYYESLLDDSIDPASIEAREDLTMFVKRHKLESELIKSAAEGLGFSYTEPNYWIRYGGRQDENRVPSGVNVEGAEVHGFSMGKKLSPKIDRDLREGGPLLYENITFVYNSEQLNGTQRVLLDNVLSEEQCRELHSVASGIMLVGDGYRGKTSPHTPNEKFEGATVLKALKSGYEGRVPLKSARLFYDISEKARRIVESYFMLNSTLYFSYTHMVCRTALSGQQDRRNDLSHPIHADNCLLDPEANECWKEPPAYTFRDYSALLYMNDDFEGGEFIFTEMDAKTVTASIKPKCGRMISFSSGGENPHGVKAVTKGKRCAVALWFTLDPLYRELERIQADEVIAILDQEQQGKHELNINPKDEL

Target ORF information:

RefSeq Version	NM_001134418.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 3-hydroxylase 2 (P3H2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134418.1

ORF Insert Sequence:

ATGGAAATGC AGCAGAACAT TGAGAATTAC AGGGCGACAG CTGGTGTTGA AGCATTGCAG 
TTGGTAGACA GAGAAGCCAA GCCACACATG GAGAGTTACA ATGCAGGAGT TAAACATTAT 
GAGGCTGATG ACTTTGAGAT GGCTATCAGG CACTTCGAAC AAGCCTTAAG AGAATATTTC 
GTTGAAGATA CAGAATGCCG GACCCTATGT GAGGGGCCTC AGAGATTTGA AGAATATGAG 
TATTTAGGGT ATAAGGCTGG TCTGTATGAA GCTATTGCAG ATCACTACAT GCAGGTGCTT 
GTTTGTCAGC ATGAATGTGT GAGGGAACTT GCCACCCGCC CTGGCCGCCT CTCTCCCATC 
GAGAATTTTC TTCCTCTGCA CTATGATTAC CTACAGTTTG CCTACTATCG AGTTGGTGAG 
TATGTGAAAG CCCTGGAGTG TGCCAAAGCC TATCTTCTAT GCCATCCAGA TGATGAGGAT 
GTCCTAGACA ATGTGGATTA CTATGAGAGT CTGCTGGATG ATAGCATTGA CCCGGCATCC 
ATTGAGGCCA GAGAGGATTT AACAATGTTT GTGAAACGTC ATAAGCTGGA GTCTGAGCTG 
ATAAAATCAG CTGCAGAAGG TCTGGGGTTT TCATACACTG AACCGAATTA TTGGATCAGA 
TATGGAGGAC GACAGGATGA GAATCGGGTC CCTTCAGGAG TGAACGTAGA GGGAGCAGAA 
GTTCATGGAT TCTCAATGGG AAAAAAGCTA TCACCCAAGA TAGATCGAGA CCTAAGAGAA 
GGTGGTCCTC TACTCTATGA GAACATCACA TTCGTCTACA ACTCGGAGCA GCTGAACGGG 
ACTCAGCGGG TTCTCCTGGA TAACGTCCTG TCGGAAGAAC AGTGCCGGGA GCTCCACAGC 
GTGGCCAGTG GAATCATGCT TGTTGGTGAT GGATACAGAG GAAAAACTTC ACCCCATACA 
CCCAATGAAA AGTTTGAAGG TGCAACTGTC CTGAAAGCAC TCAAATCTGG TTATGAAGGT 
CGAGTCCCAC TGAAGAGCGC TCGTCTGTTT TATGACATCA GCGAAAAGGC TCGAAGGATT 
GTAGAATCTT ATTTTATGCT GAACTCAACT CTGTATTTTT CCTATACACA CATGGTCTGC 
CGAACAGCCC TGTCTGGTCA GCAGGATAGA AGAAATGACC TCAGTCATCC CATCCATGCT 
GACAACTGTT TGTTGGATCC AGAGGCCAAC GAATGCTGGA AGGAGCCTCC TGCTTACACA 
TTTCGAGACT ATAGTGCTCT CCTATATATG AATGATGACT TTGAAGGAGG AGAATTCATA 
TTCACAGAGA TGGATGCTAA GACTGTGACT GCCTCTATAA AACCAAAATG TGGGCGCATG 
ATCAGCTTCT CATCTGGAGG AGAGAACCCT CATGGGGTGA AGGCAGTCAC CAAGGGAAAG 
AGGTGTGCTG TGGCTCTGTG GTTCACCTTG GACCCACTTT ATAGAGAATT GGAGCGAATA 
CAGGCTGATG AAGTGATTGC AATTCTGGAT CAAGAACAGC AAGGGAAGCA TGAACTGAAT 
ATCAACCCTA AAGATGAGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3H2 ( XM_011512955.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511257.1 Homo sapiens prolyl 3-hydroxylase 2 (P3H2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu19470
Clone ID Related Accession (Same CDS sequence)	NM_001134418.1 , XM_011512955.1
Accession Version	XM_011512955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	prolyl 3-hydroxylase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATGC AGCAGAACAT TGAGAATTAC AGGGCGACAG CTGGTGTTGA AGCATTGCAG 
TTGGTAGACA GAGAAGCCAA GCCACACATG GAGAGTTACA ATGCAGGAGT TAAACATTAT 
GAGGCTGATG ACTTTGAGAT GGCTATCAGG CACTTCGAAC AAGCCTTAAG AGAATATTTC 
GTTGAAGATA CAGAATGCCG GACCCTATGT GAGGGGCCTC AGAGATTTGA AGAATATGAG 
TATTTAGGGT ATAAGGCTGG TCTGTATGAA GCTATTGCAG ATCACTACAT GCAGGTGCTT 
GTTTGTCAGC ATGAATGTGT GAGGGAACTT GCCACCCGCC CTGGCCGCCT CTCTCCCATC 
GAGAATTTTC TTCCTCTGCA CTATGATTAC CTACAGTTTG CCTACTATCG AGTTGGTGAG 
TATGTGAAAG CCCTGGAGTG TGCCAAAGCC TATCTTCTAT GCCATCCAGA TGATGAGGAT 
GTCCTAGACA ATGTGGATTA CTATGAGAGT CTGCTGGATG ATAGCATTGA CCCGGCATCC 
ATTGAGGCCA GAGAGGATTT AACAATGTTT GTGAAACGTC ATAAGCTGGA GTCTGAGCTG 
ATAAAATCAG CTGCAGAAGG TCTGGGGTTT TCATACACTG AACCGAATTA TTGGATCAGA 
TATGGAGGAC GACAGGATGA GAATCGGGTC CCTTCAGGAG TGAACGTAGA GGGAGCAGAA 
GTTCATGGAT TCTCAATGGG AAAAAAGCTA TCACCCAAGA TAGATCGAGA CCTAAGAGAA 
GGTGGTCCTC TACTCTATGA GAACATCACA TTCGTCTACA ACTCGGAGCA GCTGAACGGG 
ACTCAGCGGG TTCTCCTGGA TAACGTCCTG TCGGAAGAAC AGTGCCGGGA GCTCCACAGC 
GTGGCCAGTG GAATCATGCT TGTTGGTGAT GGATACAGAG GAAAAACTTC ACCCCATACA 
CCCAATGAAA AGTTTGAAGG TGCAACTGTC CTGAAAGCAC TCAAATCTGG TTATGAAGGT 
CGAGTCCCAC TGAAGAGCGC TCGTCTGTTT TATGACATCA GCGAAAAGGC TCGAAGGATT 
GTAGAATCTT ATTTTATGCT GAACTCAACT CTGTATTTTT CCTATACACA CATGGTCTGC 
CGAACAGCCC TGTCTGGTCA GCAGGATAGA AGAAATGACC TCAGTCATCC CATCCATGCT 
GACAACTGTT TGTTGGATCC AGAGGCCAAC GAATGCTGGA AGGAGCCTCC TGCTTACACA 
TTTCGAGACT ATAGTGCTCT CCTATATATG AATGATGACT TTGAAGGAGG AGAATTCATA 
TTCACAGAGA TGGATGCTAA GACTGTGACT GCCTCTATAA AACCAAAATG TGGGCGCATG 
ATCAGCTTCT CATCTGGAGG AGAGAACCCT CATGGGGTGA AGGCAGTCAC CAAGGGAAAG 
AGGTGTGCTG TGGCTCTGTG GTTCACCTTG GACCCACTTT ATAGAGAATT GGAGCGAATA 
CAGGCTGATG AAGTGATTGC AATTCTGGAT CAAGAACAGC AAGGGAAGCA TGAACTGAAT 
ATCAACCCTA AAGATGAGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511257.1
CDS	206..1789
Translation	MEMQQNIENYRATAGVEALQLVDREAKPHMESYNAGVKHYEADDFEMAIRHFEQALREYFVEDTECRTLCEGPQRFEEYEYLGYKAGLYEAIADHYMQVLVCQHECVRELATRPGRLSPIENFLPLHYDYLQFAYYRVGEYVKALECAKAYLLCHPDDEDVLDNVDYYESLLDDSIDPASIEAREDLTMFVKRHKLESELIKSAAEGLGFSYTEPNYWIRYGGRQDENRVPSGVNVEGAEVHGFSMGKKLSPKIDRDLREGGPLLYENITFVYNSEQLNGTQRVLLDNVLSEEQCRELHSVASGIMLVGDGYRGKTSPHTPNEKFEGATVLKALKSGYEGRVPLKSARLFYDISEKARRIVESYFMLNSTLYFSYTHMVCRTALSGQQDRRNDLSHPIHADNCLLDPEANECWKEPPAYTFRDYSALLYMNDDFEGGEFIFTEMDAKTVTASIKPKCGRMISFSSGGENPHGVKAVTKGKRCAVALWFTLDPLYRELERIQADEVIAILDQEQQGKHELNINPKDEL

Target ORF information:

RefSeq Version	XM_011512955.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 3-hydroxylase 2 (P3H2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512955.1

ORF Insert Sequence:

ATGGAAATGC AGCAGAACAT TGAGAATTAC AGGGCGACAG CTGGTGTTGA AGCATTGCAG 
TTGGTAGACA GAGAAGCCAA GCCACACATG GAGAGTTACA ATGCAGGAGT TAAACATTAT 
GAGGCTGATG ACTTTGAGAT GGCTATCAGG CACTTCGAAC AAGCCTTAAG AGAATATTTC 
GTTGAAGATA CAGAATGCCG GACCCTATGT GAGGGGCCTC AGAGATTTGA AGAATATGAG 
TATTTAGGGT ATAAGGCTGG TCTGTATGAA GCTATTGCAG ATCACTACAT GCAGGTGCTT 
GTTTGTCAGC ATGAATGTGT GAGGGAACTT GCCACCCGCC CTGGCCGCCT CTCTCCCATC 
GAGAATTTTC TTCCTCTGCA CTATGATTAC CTACAGTTTG CCTACTATCG AGTTGGTGAG 
TATGTGAAAG CCCTGGAGTG TGCCAAAGCC TATCTTCTAT GCCATCCAGA TGATGAGGAT 
GTCCTAGACA ATGTGGATTA CTATGAGAGT CTGCTGGATG ATAGCATTGA CCCGGCATCC 
ATTGAGGCCA GAGAGGATTT AACAATGTTT GTGAAACGTC ATAAGCTGGA GTCTGAGCTG 
ATAAAATCAG CTGCAGAAGG TCTGGGGTTT TCATACACTG AACCGAATTA TTGGATCAGA 
TATGGAGGAC GACAGGATGA GAATCGGGTC CCTTCAGGAG TGAACGTAGA GGGAGCAGAA 
GTTCATGGAT TCTCAATGGG AAAAAAGCTA TCACCCAAGA TAGATCGAGA CCTAAGAGAA 
GGTGGTCCTC TACTCTATGA GAACATCACA TTCGTCTACA ACTCGGAGCA GCTGAACGGG 
ACTCAGCGGG TTCTCCTGGA TAACGTCCTG TCGGAAGAAC AGTGCCGGGA GCTCCACAGC 
GTGGCCAGTG GAATCATGCT TGTTGGTGAT GGATACAGAG GAAAAACTTC ACCCCATACA 
CCCAATGAAA AGTTTGAAGG TGCAACTGTC CTGAAAGCAC TCAAATCTGG TTATGAAGGT 
CGAGTCCCAC TGAAGAGCGC TCGTCTGTTT TATGACATCA GCGAAAAGGC TCGAAGGATT 
GTAGAATCTT ATTTTATGCT GAACTCAACT CTGTATTTTT CCTATACACA CATGGTCTGC 
CGAACAGCCC TGTCTGGTCA GCAGGATAGA AGAAATGACC TCAGTCATCC CATCCATGCT 
GACAACTGTT TGTTGGATCC AGAGGCCAAC GAATGCTGGA AGGAGCCTCC TGCTTACACA 
TTTCGAGACT ATAGTGCTCT CCTATATATG AATGATGACT TTGAAGGAGG AGAATTCATA 
TTCACAGAGA TGGATGCTAA GACTGTGACT GCCTCTATAA AACCAAAATG TGGGCGCATG 
ATCAGCTTCT CATCTGGAGG AGAGAACCCT CATGGGGTGA AGGCAGTCAC CAAGGGAAAG 
AGGTGTGCTG TGGCTCTGTG GTTCACCTTG GACCCACTTT ATAGAGAATT GGAGCGAATA 
CAGGCTGATG AAGTGATTGC AATTCTGGAT CAAGAACAGC AAGGGAAGCA TGAACTGAAT 
ATCAACCCTA AAGATGAGCT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3H2 ( NM_018192.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060662.2 Homo sapiens prolyl 3-hydroxylase 2 (P3H2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02725
Clone ID Related Accession (Same CDS sequence)	NM_018192.4 , NM_018192.3
Accession Version	NM_018192.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	prolyl 3-hydroxylase 2 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ430351.2, AK125134.1, BC005029.1, BU621129.1 and BU737886.1. On Nov 23, 2018 this sequence version replaced NM_018192.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK125134.1, AJ430351.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000319332.10/ ENSP00000316881.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGGAGC GCATCTGGGC GCCGCCGCTG CTGCTGCTGC TGCCGCTGCT ACTGCCGCCG 
CCACTGTGGG GCGGCCCCCC GGACAGCCCA CGCCGGGAGC TGGAGCTGGA GCCCGGGCCT 
CTGCAGCCCT TCGACCTGCT CTACGCCAGC GGCGCGGCCG CCTACTACAG CGGAGACTAC 
GAGCGAGCGG TGCGCGACTT GGAAGCGGCG CTGCGCAGCC ACCGGCGCCT GCGGGAAATC 
CGCACGCGCT GTGCCCGCCA CTGCGCGGCG CGCCACCCGC TCCCGCCCCC GCCCCCCGGC 
GAGGGCCCCG GCGCTGAGCT GCCCCTTTTC CGCTCCTTGT TGGGGCGGGC GCGCTGTTAT 
CGCAGCTGTG AGACCCAGCG CCTCGGGGGC CCCGCATCCC GCCACCGCGT CAGCGAGGAT 
GTGCGCAGCG ACTTCCAGCG CAGAGTGCCC TACAACTACC TGCAGCGGGC CTACATCAAG 
CTTAACCAGC TCGAAAAAGC AGTGGAAGCA GCTCACACAT TTTTCGTGGC TAACCCTGAG 
CACATGGAAA TGCAGCAGAA CATTGAGAAT TACAGGGCGA CAGCTGGTGT TGAAGCATTG 
CAGTTGGTAG ACAGAGAAGC CAAGCCACAC ATGGAGAGTT ACAATGCAGG AGTTAAACAT 
TATGAGGCTG ATGACTTTGA GATGGCTATC AGGCACTTCG AACAAGCCTT AAGAGAATAT 
TTCGTTGAAG ATACAGAATG CCGGACCCTA TGTGAGGGGC CTCAGAGATT TGAAGAATAT 
GAGTATTTAG GGTATAAGGC TGGTCTGTAT GAAGCTATTG CAGATCACTA CATGCAGGTG 
CTTGTTTGTC AGCATGAATG TGTGAGGGAA CTTGCCACCC GCCCTGGCCG CCTCTCTCCC 
ATCGAGAATT TTCTTCCTCT GCACTATGAT TACCTACAGT TTGCCTACTA TCGAGTTGGT 
GAGTATGTGA AAGCCCTGGA GTGTGCCAAA GCCTATCTTC TATGCCATCC AGATGATGAG 
GATGTCCTAG ACAATGTGGA TTACTATGAG AGTCTGCTGG ATGATAGCAT TGACCCGGCA 
TCCATTGAGG CCAGAGAGGA TTTAACAATG TTTGTGAAAC GTCATAAGCT GGAGTCTGAG 
CTGATAAAAT CAGCTGCAGA AGGTCTGGGG TTTTCATACA CTGAACCGAA TTATTGGATC 
AGATATGGAG GACGACAGGA TGAGAATCGG GTCCCTTCAG GAGTGAACGT AGAGGGAGCA 
GAAGTTCATG GATTCTCAAT GGGAAAAAAG CTATCACCCA AGATAGATCG AGACCTAAGA 
GAAGGTGGTC CTCTACTCTA TGAGAACATC ACATTCGTCT ACAACTCGGA GCAGCTGAAC 
GGGACTCAGC GGGTTCTCCT GGATAACGTC CTGTCGGAAG AACAGTGCCG GGAGCTCCAC 
AGCGTGGCCA GTGGAATCAT GCTTGTTGGT GATGGATACA GAGGAAAAAC TTCACCCCAT 
ACACCCAATG AAAAGTTTGA AGGTGCAACT GTCCTGAAAG CACTCAAATC TGGTTATGAA 
GGTCGAGTCC CACTGAAGAG CGCTCGTCTG TTTTATGACA TCAGCGAAAA GGCTCGAAGG 
ATTGTAGAAT CTTATTTTAT GCTGAACTCA ACTCTGTATT TTTCCTATAC ACACATGGTC 
TGCCGAACAG CCCTGTCTGG TCAGCAGGAT AGAAGAAATG ACCTCAGTCA TCCCATCCAT 
GCTGACAACT GTTTGTTGGA TCCAGAGGCC AACGAATGCT GGAAGGAGCC TCCTGCTTAC 
ACATTTCGAG ACTATAGTGC TCTCCTATAT ATGAATGATG ACTTTGAAGG AGGAGAATTC 
ATATTCACAG AGATGGATGC TAAGACTGTG ACTGCCTCTA TAAAACCAAA ATGTGGGCGC 
ATGATCAGCT TCTCATCTGG AGGAGAGAAC CCTCATGGGG TGAAGGCAGT CACCAAGGGA 
AAGAGGTGTG CTGTGGCTCT GTGGTTCACC TTGGACCCAC TTTATAGAGA ATTGGAGCGA 
ATACAGGCTG ATGAAGTGAT TGCAATTCTG GATCAAGAAC AGCAAGGGAA GCATGAACTG 
AATATCAACC CTAAAGATGA GCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060662.2
CDS	167..2293
Translation	MRERIWAPPLLLLLPLLLPPPLWGGPPDSPRRELELEPGPLQPFDLLYASGAAAYYSGDYERAVRDLEAALRSHRRLREIRTRCARHCAARHPLPPPPPGEGPGAELPLFRSLLGRARCYRSCETQRLGGPASRHRVSEDVRSDFQRRVPYNYLQRAYIKLNQLEKAVEAAHTFFVANPEHMEMQQNIENYRATAGVEALQLVDREAKPHMESYNAGVKHYEADDFEMAIRHFEQALREYFVEDTECRTLCEGPQRFEEYEYLGYKAGLYEAIADHYMQVLVCQHECVRELATRPGRLSPIENFLPLHYDYLQFAYYRVGEYVKALECAKAYLLCHPDDEDVLDNVDYYESLLDDSIDPASIEAREDLTMFVKRHKLESELIKSAAEGLGFSYTEPNYWIRYGGRQDENRVPSGVNVEGAEVHGFSMGKKLSPKIDRDLREGGPLLYENITFVYNSEQLNGTQRVLLDNVLSEEQCRELHSVASGIMLVGDGYRGKTSPHTPNEKFEGATVLKALKSGYEGRVPLKSARLFYDISEKARRIVESYFMLNSTLYFSYTHMVCRTALSGQQDRRNDLSHPIHADNCLLDPEANECWKEPPAYTFRDYSALLYMNDDFEGGEFIFTEMDAKTVTASIKPKCGRMISFSSGGENPHGVKAVTKGKRCAVALWFTLDPLYRELERIQADEVIAILDQEQQGKHELNINPKDEL

Target ORF information:

RefSeq Version	NM_018192.4
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 3-hydroxylase 2 (P3H2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018192.4

ORF Insert Sequence:

ATGCGGGAGC GCATCTGGGC GCCGCCGCTG CTGCTGCTGC TGCCGCTGCT ACTGCCGCCG 
CCACTGTGGG GCGGCCCCCC GGACAGCCCA CGCCGGGAGC TGGAGCTGGA GCCCGGGCCT 
CTGCAGCCCT TCGACCTGCT CTACGCCAGC GGCGCGGCCG CCTACTACAG CGGAGACTAC 
GAGCGAGCGG TGCGCGACTT GGAAGCGGCG CTGCGCAGCC ACCGGCGCCT GCGGGAAATC 
CGCACGCGCT GTGCCCGCCA CTGCGCGGCG CGCCACCCGC TCCCGCCCCC GCCCCCCGGC 
GAGGGCCCCG GCGCTGAGCT GCCCCTTTTC CGCTCCTTGT TGGGGCGGGC GCGCTGTTAT 
CGCAGCTGTG AGACCCAGCG CCTCGGGGGC CCCGCATCCC GCCACCGCGT CAGCGAGGAT 
GTGCGCAGCG ACTTCCAGCG CAGAGTGCCC TACAACTACC TGCAGCGGGC CTACATCAAG 
CTTAACCAGC TCGAAAAAGC AGTGGAAGCA GCTCACACAT TTTTCGTGGC TAACCCTGAG 
CACATGGAAA TGCAGCAGAA CATTGAGAAT TACAGGGCGA CAGCTGGTGT TGAAGCATTG 
CAGTTGGTAG ACAGAGAAGC CAAGCCACAC ATGGAGAGTT ACAATGCAGG AGTTAAACAT 
TATGAGGCTG ATGACTTTGA GATGGCTATC AGGCACTTCG AACAAGCCTT AAGAGAATAT 
TTCGTTGAAG ATACAGAATG CCGGACCCTA TGTGAGGGGC CTCAGAGATT TGAAGAATAT 
GAGTATTTAG GGTATAAGGC TGGTCTGTAT GAAGCTATTG CAGATCACTA CATGCAGGTG 
CTTGTTTGTC AGCATGAATG TGTGAGGGAA CTTGCCACCC GCCCTGGCCG CCTCTCTCCC 
ATCGAGAATT TTCTTCCTCT GCACTATGAT TACCTACAGT TTGCCTACTA TCGAGTTGGT 
GAGTATGTGA AAGCCCTGGA GTGTGCCAAA GCCTATCTTC TATGCCATCC AGATGATGAG 
GATGTCCTAG ACAATGTGGA TTACTATGAG AGTCTGCTGG ATGATAGCAT TGACCCGGCA 
TCCATTGAGG CCAGAGAGGA TTTAACAATG TTTGTGAAAC GTCATAAGCT GGAGTCTGAG 
CTGATAAAAT CAGCTGCAGA AGGTCTGGGG TTTTCATACA CTGAACCGAA TTATTGGATC 
AGATATGGAG GACGACAGGA TGAGAATCGG GTCCCTTCAG GAGTGAACGT AGAGGGAGCA 
GAAGTTCATG GATTCTCAAT GGGAAAAAAG CTATCACCCA AGATAGATCG AGACCTAAGA 
GAAGGTGGTC CTCTACTCTA TGAGAACATC ACATTCGTCT ACAACTCGGA GCAGCTGAAC 
GGGACTCAGC GGGTTCTCCT GGATAACGTC CTGTCGGAAG AACAGTGCCG GGAGCTCCAC 
AGCGTGGCCA GTGGAATCAT GCTTGTTGGT GATGGATACA GAGGAAAAAC TTCACCCCAT 
ACACCCAATG AAAAGTTTGA AGGTGCAACT GTCCTGAAAG CACTCAAATC TGGTTATGAA 
GGTCGAGTCC CACTGAAGAG CGCTCGTCTG TTTTATGACA TCAGCGAAAA GGCTCGAAGG 
ATTGTAGAAT CTTATTTTAT GCTGAACTCA ACTCTGTATT TTTCCTATAC ACACATGGTC 
TGCCGAACAG CCCTGTCTGG TCAGCAGGAT AGAAGAAATG ACCTCAGTCA TCCCATCCAT 
GCTGACAACT GTTTGTTGGA TCCAGAGGCC AACGAATGCT GGAAGGAGCC TCCTGCTTAC 
ACATTTCGAG ACTATAGTGC TCTCCTATAT ATGAATGATG ACTTTGAAGG AGGAGAATTC 
ATATTCACAG AGATGGATGC TAAGACTGTG ACTGCCTCTA TAAAACCAAA ATGTGGGCGC 
ATGATCAGCT TCTCATCTGG AGGAGAGAAC CCTCATGGGG TGAAGGCAGT CACCAAGGGA 
AAGAGGTGTG CTGTGGCTCT GTGGTTCACC TTGGACCCAC TTTATAGAGA ATTGGAGCGA 
ATACAGGCTG ATGAAGTGAT TGCAATTCTG GATCAAGAAC AGCAAGGGAA GCATGAACTG 
AATATCAACC CTAAAGATGA GCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3H2 ( NM_018192.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060662.2 Homo sapiens prolyl 3-hydroxylase 2 (P3H2), transcript variant 1, mRNA.

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CloneID	OHu02725
Clone ID Related Accession (Same CDS sequence)	NM_018192.4 , NM_018192.3
Accession Version	NM_018192.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	prolyl 3-hydroxylase 2 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA879789.1, AJ430351.2, AK125134.1, BC005029.1, BU621129.1 and BU737886.1. On Aug 28, 2008 this sequence version replaced NM_018192.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: AK125134.1, AJ430351.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGAGC GCATCTGGGC GCCGCCGCTG CTGCTGCTGC TGCCGCTGCT ACTGCCGCCG 
CCACTGTGGG GCGGCCCCCC GGACAGCCCA CGCCGGGAGC TGGAGCTGGA GCCCGGGCCT 
CTGCAGCCCT TCGACCTGCT CTACGCCAGC GGCGCGGCCG CCTACTACAG CGGAGACTAC 
GAGCGAGCGG TGCGCGACTT GGAAGCGGCG CTGCGCAGCC ACCGGCGCCT GCGGGAAATC 
CGCACGCGCT GTGCCCGCCA CTGCGCGGCG CGCCACCCGC TCCCGCCCCC GCCCCCCGGC 
GAGGGCCCCG GCGCTGAGCT GCCCCTTTTC CGCTCCTTGT TGGGGCGGGC GCGCTGTTAT 
CGCAGCTGTG AGACCCAGCG CCTCGGGGGC CCCGCATCCC GCCACCGCGT CAGCGAGGAT 
GTGCGCAGCG ACTTCCAGCG CAGAGTGCCC TACAACTACC TGCAGCGGGC CTACATCAAG 
CTTAACCAGC TCGAAAAAGC AGTGGAAGCA GCTCACACAT TTTTCGTGGC TAACCCTGAG 
CACATGGAAA TGCAGCAGAA CATTGAGAAT TACAGGGCGA CAGCTGGTGT TGAAGCATTG 
CAGTTGGTAG ACAGAGAAGC CAAGCCACAC ATGGAGAGTT ACAATGCAGG AGTTAAACAT 
TATGAGGCTG ATGACTTTGA GATGGCTATC AGGCACTTCG AACAAGCCTT AAGAGAATAT 
TTCGTTGAAG ATACAGAATG CCGGACCCTA TGTGAGGGGC CTCAGAGATT TGAAGAATAT 
GAGTATTTAG GGTATAAGGC TGGTCTGTAT GAAGCTATTG CAGATCACTA CATGCAGGTG 
CTTGTTTGTC AGCATGAATG TGTGAGGGAA CTTGCCACCC GCCCTGGCCG CCTCTCTCCC 
ATCGAGAATT TTCTTCCTCT GCACTATGAT TACCTACAGT TTGCCTACTA TCGAGTTGGT 
GAGTATGTGA AAGCCCTGGA GTGTGCCAAA GCCTATCTTC TATGCCATCC AGATGATGAG 
GATGTCCTAG ACAATGTGGA TTACTATGAG AGTCTGCTGG ATGATAGCAT TGACCCGGCA 
TCCATTGAGG CCAGAGAGGA TTTAACAATG TTTGTGAAAC GTCATAAGCT GGAGTCTGAG 
CTGATAAAAT CAGCTGCAGA AGGTCTGGGG TTTTCATACA CTGAACCGAA TTATTGGATC 
AGATATGGAG GACGACAGGA TGAGAATCGG GTCCCTTCAG GAGTGAACGT AGAGGGAGCA 
GAAGTTCATG GATTCTCAAT GGGAAAAAAG CTATCACCCA AGATAGATCG AGACCTAAGA 
GAAGGTGGTC CTCTACTCTA TGAGAACATC ACATTCGTCT ACAACTCGGA GCAGCTGAAC 
GGGACTCAGC GGGTTCTCCT GGATAACGTC CTGTCGGAAG AACAGTGCCG GGAGCTCCAC 
AGCGTGGCCA GTGGAATCAT GCTTGTTGGT GATGGATACA GAGGAAAAAC TTCACCCCAT 
ACACCCAATG AAAAGTTTGA AGGTGCAACT GTCCTGAAAG CACTCAAATC TGGTTATGAA 
GGTCGAGTCC CACTGAAGAG CGCTCGTCTG TTTTATGACA TCAGCGAAAA GGCTCGAAGG 
ATTGTAGAAT CTTATTTTAT GCTGAACTCA ACTCTGTATT TTTCCTATAC ACACATGGTC 
TGCCGAACAG CCCTGTCTGG TCAGCAGGAT AGAAGAAATG ACCTCAGTCA TCCCATCCAT 
GCTGACAACT GTTTGTTGGA TCCAGAGGCC AACGAATGCT GGAAGGAGCC TCCTGCTTAC 
ACATTTCGAG ACTATAGTGC TCTCCTATAT ATGAATGATG ACTTTGAAGG AGGAGAATTC 
ATATTCACAG AGATGGATGC TAAGACTGTG ACTGCCTCTA TAAAACCAAA ATGTGGGCGC 
ATGATCAGCT TCTCATCTGG AGGAGAGAAC CCTCATGGGG TGAAGGCAGT CACCAAGGGA 
AAGAGGTGTG CTGTGGCTCT GTGGTTCACC TTGGACCCAC TTTATAGAGA ATTGGAGCGA 
ATACAGGCTG ATGAAGTGAT TGCAATTCTG GATCAAGAAC AGCAAGGGAA GCATGAACTG 
AATATCAACC CTAAAGATGA GCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060662.2
CDS	389..2515
Translation	MRERIWAPPLLLLLPLLLPPPLWGGPPDSPRRELELEPGPLQPFDLLYASGAAAYYSGDYERAVRDLEAALRSHRRLREIRTRCARHCAARHPLPPPPPGEGPGAELPLFRSLLGRARCYRSCETQRLGGPASRHRVSEDVRSDFQRRVPYNYLQRAYIKLNQLEKAVEAAHTFFVANPEHMEMQQNIENYRATAGVEALQLVDREAKPHMESYNAGVKHYEADDFEMAIRHFEQALREYFVEDTECRTLCEGPQRFEEYEYLGYKAGLYEAIADHYMQVLVCQHECVRELATRPGRLSPIENFLPLHYDYLQFAYYRVGEYVKALECAKAYLLCHPDDEDVLDNVDYYESLLDDSIDPASIEAREDLTMFVKRHKLESELIKSAAEGLGFSYTEPNYWIRYGGRQDENRVPSGVNVEGAEVHGFSMGKKLSPKIDRDLREGGPLLYENITFVYNSEQLNGTQRVLLDNVLSEEQCRELHSVASGIMLVGDGYRGKTSPHTPNEKFEGATVLKALKSGYEGRVPLKSARLFYDISEKARRIVESYFMLNSTLYFSYTHMVCRTALSGQQDRRNDLSHPIHADNCLLDPEANECWKEPPAYTFRDYSALLYMNDDFEGGEFIFTEMDAKTVTASIKPKCGRMISFSSGGENPHGVKAVTKGKRCAVALWFTLDPLYRELERIQADEVIAILDQEQQGKHELNINPKDEL

Target ORF information:

RefSeq Version	NM_018192.3
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 3-hydroxylase 2 (P3H2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018192.3

ORF Insert Sequence:

ATGCGGGAGC GCATCTGGGC GCCGCCGCTG CTGCTGCTGC TGCCGCTGCT ACTGCCGCCG 
CCACTGTGGG GCGGCCCCCC GGACAGCCCA CGCCGGGAGC TGGAGCTGGA GCCCGGGCCT 
CTGCAGCCCT TCGACCTGCT CTACGCCAGC GGCGCGGCCG CCTACTACAG CGGAGACTAC 
GAGCGAGCGG TGCGCGACTT GGAAGCGGCG CTGCGCAGCC ACCGGCGCCT GCGGGAAATC 
CGCACGCGCT GTGCCCGCCA CTGCGCGGCG CGCCACCCGC TCCCGCCCCC GCCCCCCGGC 
GAGGGCCCCG GCGCTGAGCT GCCCCTTTTC CGCTCCTTGT TGGGGCGGGC GCGCTGTTAT 
CGCAGCTGTG AGACCCAGCG CCTCGGGGGC CCCGCATCCC GCCACCGCGT CAGCGAGGAT 
GTGCGCAGCG ACTTCCAGCG CAGAGTGCCC TACAACTACC TGCAGCGGGC CTACATCAAG 
CTTAACCAGC TCGAAAAAGC AGTGGAAGCA GCTCACACAT TTTTCGTGGC TAACCCTGAG 
CACATGGAAA TGCAGCAGAA CATTGAGAAT TACAGGGCGA CAGCTGGTGT TGAAGCATTG 
CAGTTGGTAG ACAGAGAAGC CAAGCCACAC ATGGAGAGTT ACAATGCAGG AGTTAAACAT 
TATGAGGCTG ATGACTTTGA GATGGCTATC AGGCACTTCG AACAAGCCTT AAGAGAATAT 
TTCGTTGAAG ATACAGAATG CCGGACCCTA TGTGAGGGGC CTCAGAGATT TGAAGAATAT 
GAGTATTTAG GGTATAAGGC TGGTCTGTAT GAAGCTATTG CAGATCACTA CATGCAGGTG 
CTTGTTTGTC AGCATGAATG TGTGAGGGAA CTTGCCACCC GCCCTGGCCG CCTCTCTCCC 
ATCGAGAATT TTCTTCCTCT GCACTATGAT TACCTACAGT TTGCCTACTA TCGAGTTGGT 
GAGTATGTGA AAGCCCTGGA GTGTGCCAAA GCCTATCTTC TATGCCATCC AGATGATGAG 
GATGTCCTAG ACAATGTGGA TTACTATGAG AGTCTGCTGG ATGATAGCAT TGACCCGGCA 
TCCATTGAGG CCAGAGAGGA TTTAACAATG TTTGTGAAAC GTCATAAGCT GGAGTCTGAG 
CTGATAAAAT CAGCTGCAGA AGGTCTGGGG TTTTCATACA CTGAACCGAA TTATTGGATC 
AGATATGGAG GACGACAGGA TGAGAATCGG GTCCCTTCAG GAGTGAACGT AGAGGGAGCA 
GAAGTTCATG GATTCTCAAT GGGAAAAAAG CTATCACCCA AGATAGATCG AGACCTAAGA 
GAAGGTGGTC CTCTACTCTA TGAGAACATC ACATTCGTCT ACAACTCGGA GCAGCTGAAC 
GGGACTCAGC GGGTTCTCCT GGATAACGTC CTGTCGGAAG AACAGTGCCG GGAGCTCCAC 
AGCGTGGCCA GTGGAATCAT GCTTGTTGGT GATGGATACA GAGGAAAAAC TTCACCCCAT 
ACACCCAATG AAAAGTTTGA AGGTGCAACT GTCCTGAAAG CACTCAAATC TGGTTATGAA 
GGTCGAGTCC CACTGAAGAG CGCTCGTCTG TTTTATGACA TCAGCGAAAA GGCTCGAAGG 
ATTGTAGAAT CTTATTTTAT GCTGAACTCA ACTCTGTATT TTTCCTATAC ACACATGGTC 
TGCCGAACAG CCCTGTCTGG TCAGCAGGAT AGAAGAAATG ACCTCAGTCA TCCCATCCAT 
GCTGACAACT GTTTGTTGGA TCCAGAGGCC AACGAATGCT GGAAGGAGCC TCCTGCTTAC 
ACATTTCGAG ACTATAGTGC TCTCCTATAT ATGAATGATG ACTTTGAAGG AGGAGAATTC 
ATATTCACAG AGATGGATGC TAAGACTGTG ACTGCCTCTA TAAAACCAAA ATGTGGGCGC 
ATGATCAGCT TCTCATCTGG AGGAGAGAAC CCTCATGGGG TGAAGGCAGT CACCAAGGGA 
AAGAGGTGTG CTGTGGCTCT GTGGTTCACC TTGGACCCAC TTTATAGAGA ATTGGAGCGA 
ATACAGGCTG ATGAAGTGAT TGCAATTCTG GATCAAGAAC AGCAAGGGAA GCATGAACTG 
AATATCAACC CTAAAGATGA GCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Recessive mutations in LEPREL1 underlie a recognizable lens subluxation phenotype.
Ophthalmic genetics36(1)58-63(2015 Mar)
Khan AO,Aldahmesh MA,Alsharif H,Alkuraya FS

Homozygous loss-of-function mutation of the LEPREL1 gene causes severe non-syndromic high myopia with early-onset cataract.
Clinical genetics86(6)575-9(2014 Dec)
Guo H,Tong P,Peng Y,Wang T,Liu Y,Chen J,Li Y,Tian Q,Hu Y,Zheng Y,Xiao L,Xiong W,Pan Q,Hu Z,Xia K

Detection of mutations in LRPAP1, CTSH, LEPREL1, ZNF644, SLC39A5, and SCO2 in 298 families with early-onset high myopia by exome sequencing.
Investigative ophthalmology & visual science56(1)339-45(2014 Dec)
Jiang D,Li J,Xiao X,Li S,Jia X,Sun W,Guo X,Zhang Q

High myopia caused by a mutation in LEPREL1, encoding prolyl 3-hydroxylase 2.
American journal of human genetics89(3)438-45(2011 Sep)
Mordechai S,Gradstein L,Pasanen A,Ofir R,El Amour K,Levy J,Belfair N,Lifshitz T,Joshua S,Narkis G,Elbedour K,Myllyharju J,Birk OS

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P3H2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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