PNPO cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PNPO
Entrez Gene ID	55163
Full Name	pyridoxamine 5'-phosphate oxidase
Synonyms	HEL-S-302,PDXPO
General protein information	Preferred Names pyridoxamine 5'-phosphate oxidase Names pyridoxine-5'-phosphate oxidase epididymis secretory protein Li 302 pyridoxal 5'-phosphate synthase pyridoxamine-phosphate oxidase pyridoxine 5'-phosphate oxidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.32
Summary	The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq, Oct 2008].
Disorder MIM:	603287
Disorder Html:	Pyridoxamine 5'-phosphate oxidase deficiency, 610090 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_018129.4	NP_060599.1	pyridoxine-5'-phosphate oxidase
XM_011524968.2	XP_011523270.1	pyridoxine-5'-phosphate oxidase isoform X2
XM_017024813.1	XP_016880302.1	pyridoxine-5'-phosphate oxidase isoform X1
NM_018129.3	NP_060599.1	pyridoxine-5'-phosphate oxidase
XM_005257500.3	XP_005257557.1	pyridoxine-5'-phosphate oxidase isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PNPO	XP_001173409.1
Mus musculus (house mouse)	Pnpo	NP_598782.1
Rattus norvegicus (Norway rat)	Pnpo	NP_072123.1
Homo sapiens (human)	PNPO	NP_060599.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002227	XP_307957.5
Saccharomyces cerevisiae (baker's yeast)	PDX3	NP_009591.1
	PNPO	XP_001084391.1
Drosophila melanogaster (fruit fly)	CG31472	NP_731186.2
Eremothecium gossypii	AGOS_AAR186W	NP_982729.1
Canis lupus familiaris (dog)	PNPO	XP_537661.2
Gallus gallus (chicken)	PNPO	XP_003643821.2
Danio rerio (zebrafish)	pnpo	NP_001243107.1
Caenorhabditis elegans (roundworm)	F57B9.1	NP_498518.2
Kluyveromyces lactis	KLLA0B07062g	XP_451844.1
Magnaporthe oryzae (rice blast fungus)	MGG_01535	XP_003714509.1
Neurospora crassa	NCU08269	XP_961897.1
Bos taurus (cattle)	PNPO	NP_001014907.1
Schizosaccharomyces pombe (fission yeast)	SPAC1093.02	NP_594650.1
Xenopus tropicalis (tropical clawed frog)	pnpo	NP_001120016.1

Gene Ontology
Bibliography

Related articles in PubMed

Pyridoxine 5'-phosphate oxidase is a novel therapeutic target and regulated by the TGF-? signalling pathway in epithelial ovarian cancer.
Zhang L, Zhou D, Guan W, Ren W, Sun W, Shi J, Lin Q, Zhang J, Qiao T, Ye Y, Wu Y, Zhang Y, Zuo X, Connor KL, Xu G
Cell death & disease8(12)3214(2017 Dec)

Pyridoxine-5'-phosphate oxidase (Pnpo) deficiency: Clinical and biochemical alterations associated with the C.347g>A (P.?Arg116gln) mutation.
di Salvo ML, Mastrangelo M, Nogu?s I, Tolve M, Paiardini A, Carducci C, Mei D, Montomoli M, Tramonti A, Guerrini R, Contestabile R, Leuzzi V
Molecular genetics and metabolism122(1-2)135-142(2017 Sep)

Pyridoxal 5?-phosphate-responsive epilepsy with novel mutations in the PNPO gene: a case report.
Veeravigrom M, Damrongphol P, Ittiwut R, Ittiwut C, Suphapeetiporn K, Shotelersuk V
Genetics and molecular research : GMR14(4)14130-5(2015 Oct)

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Pyridoxine responsiveness in novel mutations of the PNPO gene.
Plecko B, Paul K, Mills P, Clayton P, Paschke E, Maier O, Hasselmann O, Schmiedel G, Kanz S, Connolly M, Wolf N, Struys E, Stockler S, Abela L, Hofer D
Neurology82(16)1425-33(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Exome sequencing revealed that the patient was compound heterozygous for pathogenic mutations [c.546+1G>A (IVS5+1 G>A) and c.620delG (p.G207VfsX215)] in the PNPO gene.
Title: Pyridoxal 5?-phosphate-responsive epilepsy with novel mutations in the PNPO gene: a case report.

One sequence variant, R116Q, a single nucleotide polymorphism that has been reported in the general population, was found to have an effect on PNPO activity.
Title: Epilepsy due to PNPO mutations: genotype, environment and treatment affect presentation and outcome.

Challenge to the paradigm of exclusive PLP responsiveness in patients with pyridoxal 5'-phosphate oxidase deficiency and underlines the importance of consecutive testing of pyridoxine and PLP in neonates with antiepileptic drug-resistant seizures.
Title: Pyridoxine responsiveness in novel mutations of the PNPO gene.

Generalized epilepsies implicates susceptibility genes: CHRM3 at 1q43, VRK2 at 2p16.1, ZEB2 at 2q22.3, SCN1A at 2q24.3 and PNPO at 17q21.32.
Title: Genome-wide association analysis of genetic generalized epilepsies implicates susceptibility loci at 1q43, 2p16.1, 2q22.3 and 17q21.32.

provide evidence that a single arginine residue of the C terminus of pyridoxal 5'-phosphate synthase is responsible for coordinating co-operativity in this elaborate protein machinery
Title: It takes two to tango: defining an essential second active site in pyridoxal 5'-phosphate synthase.

The following PNPO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PNPO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18470	NM_018129.4 Latest version!	Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu50514	XM_005257500.3 Latest version!	Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu50514	XM_017024813.1 Latest version!	Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu69098	XM_011524968.2 Latest version!	Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OHu18470	NM_018129.3	Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PNPO ( NM_018129.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060599.1 Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), mRNA.

Price & Availability↑

CloneID	OHu18470
Clone ID Related Accession (Same CDS sequence)	NM_018129.4 , NM_018129.3
Accession Version	NM_018129.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	pyridoxine-5'-phosphate oxidase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK001397.1, BC006525.2, AC018521.8 and CB853441.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_018129.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK001397.1, SRR7346977.1085016.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACGTGCT GGCTGCGGGG CGTCACGGCG ACGTTCGGGC GACCTGCCGA GTGGCCAGGC 
TACCTCAGTC ACCTGTGTGG TCGCAGTGCT GCCATGGACC TGGGACCCAT GCGCAAGAGT 
TACCGCGGGG ACCGAGAGGC ATTTGAGGAG ACTCATCTGA CCTCCCTTGA CCCAGTGAAA 
CAGTTTGCTG CCTGGTTTGA GGAGGCTGTT CAGTGTCCTG ACATAGGGGA AGCCAATGCC 
ATGTGTCTGG CTACCTGCAC CAGAGATGGA AAACCCTCTG CTCGCATGTT GCTGCTGAAG 
GGCTTCGGGA AAGATGGCTT CCGCTTCTTC ACTAACTTCG AGAGTCGAAA AGGAAAAGAG 
CTGGACTCTA ATCCCTTTGC TTCCCTTGTC TTCTACTGGG AGCCACTTAA CCGTCAGGTG 
CGTGTGGAAG GCCCTGTGAA GAAACTGCCT GAGGAGGAGG CTGAGTGCTA CTTCCACTCC 
CGCCCCAAGA GCAGCCAGAT TGGGGCTGTG GTCAGCCACC AGAGTTCTGT GATCCCTGAT 
CGGGAGTATC TGAGAAAGAA AAATGAGGAA CTGGAACAGC TCTACCAGGA TCAAGAGGTG 
CCCAAGCCAA AATCCTGGGG TGGCTATGTC CTGTACCCTC AGGTGATGGA GTTCTGGCAA 
GGTCAAACCA ACCGCCTGCA TGACCGGATA GTCTTTCGGC GGGGCCTACC CACAGGAGAT 
TCCCCTTTGG GGCCCATGAC CCACCGCGGG GAGGAAGACT GGCTCTATGA GAGACTTGCA 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060599.1
CDS	106..891
Translation	MTCWLRGVTATFGRPAEWPGYLSHLCGRSAAMDLGPMRKSYRGDREAFEETHLTSLDPVKQFAAWFEEAVQCPDIGEANAMCLATCTRDGKPSARMLLLKGFGKDGFRFFTNFESRKGKELDSNPFASLVFYWEPLNRQVRVEGPVKKLPEEEAECYFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYVLYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP

Target ORF information:

RefSeq Version	NM_018129.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018129.4

ORF Insert Sequence:

ATGACGTGCT GGCTGCGGGG CGTCACGGCG ACGTTCGGGC GACCTGCCGA GTGGCCAGGC 
TACCTCAGTC ACCTGTGTGG TCGCAGTGCT GCCATGGACC TGGGACCCAT GCGCAAGAGT 
TACCGCGGGG ACCGAGAGGC ATTTGAGGAG ACTCATCTGA CCTCCCTTGA CCCAGTGAAA 
CAGTTTGCTG CCTGGTTTGA GGAGGCTGTT CAGTGTCCTG ACATAGGGGA AGCCAATGCC 
ATGTGTCTGG CTACCTGCAC CAGAGATGGA AAACCCTCTG CTCGCATGTT GCTGCTGAAG 
GGCTTCGGGA AAGATGGCTT CCGCTTCTTC ACTAACTTCG AGAGTCGAAA AGGAAAAGAG 
CTGGACTCTA ATCCCTTTGC TTCCCTTGTC TTCTACTGGG AGCCACTTAA CCGTCAGGTG 
CGTGTGGAAG GCCCTGTGAA GAAACTGCCT GAGGAGGAGG CTGAGTGCTA CTTCCACTCC 
CGCCCCAAGA GCAGCCAGAT TGGGGCTGTG GTCAGCCACC AGAGTTCTGT GATCCCTGAT 
CGGGAGTATC TGAGAAAGAA AAATGAGGAA CTGGAACAGC TCTACCAGGA TCAAGAGGTG 
CCCAAGCCAA AATCCTGGGG TGGCTATGTC CTGTACCCTC AGGTGATGGA GTTCTGGCAA 
GGTCAAACCA ACCGCCTGCA TGACCGGATA GTCTTTCGGC GGGGCCTACC CACAGGAGAT 
TCCCCTTTGG GGCCCATGAC CCACCGCGGG GAGGAAGACT GGCTCTATGA GAGACTTGCA 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPO ( NM_018129.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060599.1 Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), mRNA.

Price & Availability↑

CloneID	OHu18470
Clone ID Related Accession (Same CDS sequence)	NM_018129.4 , NM_018129.3
Accession Version	NM_018129.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	pyridoxine-5'-phosphate oxidase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB228906.1, AK001397.1, BC006525.2, AC018521.8 and CB853441.1. This sequence is a reference standard in the RefSeqGene project. On Oct 7, 2008 this sequence version replaced NM_018129.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.242065.1, SRR1803613.61067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGTGCT GGCTGCGGGG CGTCACGGCG ACGTTCGGGC GACCTGCCGA GTGGCCAGGC 
TACCTCAGTC ACCTGTGTGG TCGCAGTGCT GCCATGGACC TGGGACCCAT GCGCAAGAGT 
TACCGCGGGG ACCGAGAGGC ATTTGAGGAG ACTCATCTGA CCTCCCTTGA CCCAGTGAAA 
CAGTTTGCTG CCTGGTTTGA GGAGGCTGTT CAGTGTCCTG ACATAGGGGA AGCCAATGCC 
ATGTGTCTGG CTACCTGCAC CAGAGATGGA AAACCCTCTG CTCGCATGTT GCTGCTGAAG 
GGCTTCGGGA AAGATGGCTT CCGCTTCTTC ACTAACTTCG AGAGTCGAAA AGGAAAAGAG 
CTGGACTCTA ATCCCTTTGC TTCCCTTGTC TTCTACTGGG AGCCACTTAA CCGTCAGGTG 
CGTGTGGAAG GCCCTGTGAA GAAACTGCCT GAGGAGGAGG CTGAGTGCTA CTTCCACTCC 
CGCCCCAAGA GCAGCCAGAT TGGGGCTGTG GTCAGCCACC AGAGTTCTGT GATCCCTGAT 
CGGGAGTATC TGAGAAAGAA AAATGAGGAA CTGGAACAGC TCTACCAGGA TCAAGAGGTG 
CCCAAGCCAA AATCCTGGGG TGGCTATGTC CTGTACCCTC AGGTGATGGA GTTCTGGCAA 
GGTCAAACCA ACCGCCTGCA TGACCGGATA GTCTTTCGGC GGGGCCTACC CACAGGAGAT 
TCCCCTTTGG GGCCCATGAC CCACCGCGGG GAGGAAGACT GGCTCTATGA GAGACTTGCA 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060599.1
CDS	154..939
Translation	MTCWLRGVTATFGRPAEWPGYLSHLCGRSAAMDLGPMRKSYRGDREAFEETHLTSLDPVKQFAAWFEEAVQCPDIGEANAMCLATCTRDGKPSARMLLLKGFGKDGFRFFTNFESRKGKELDSNPFASLVFYWEPLNRQVRVEGPVKKLPEEEAECYFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYVLYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP

Target ORF information:

RefSeq Version	NM_018129.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018129.3

ORF Insert Sequence:

ATGACGTGCT GGCTGCGGGG CGTCACGGCG ACGTTCGGGC GACCTGCCGA GTGGCCAGGC 
TACCTCAGTC ACCTGTGTGG TCGCAGTGCT GCCATGGACC TGGGACCCAT GCGCAAGAGT 
TACCGCGGGG ACCGAGAGGC ATTTGAGGAG ACTCATCTGA CCTCCCTTGA CCCAGTGAAA 
CAGTTTGCTG CCTGGTTTGA GGAGGCTGTT CAGTGTCCTG ACATAGGGGA AGCCAATGCC 
ATGTGTCTGG CTACCTGCAC CAGAGATGGA AAACCCTCTG CTCGCATGTT GCTGCTGAAG 
GGCTTCGGGA AAGATGGCTT CCGCTTCTTC ACTAACTTCG AGAGTCGAAA AGGAAAAGAG 
CTGGACTCTA ATCCCTTTGC TTCCCTTGTC TTCTACTGGG AGCCACTTAA CCGTCAGGTG 
CGTGTGGAAG GCCCTGTGAA GAAACTGCCT GAGGAGGAGG CTGAGTGCTA CTTCCACTCC 
CGCCCCAAGA GCAGCCAGAT TGGGGCTGTG GTCAGCCACC AGAGTTCTGT GATCCCTGAT 
CGGGAGTATC TGAGAAAGAA AAATGAGGAA CTGGAACAGC TCTACCAGGA TCAAGAGGTG 
CCCAAGCCAA AATCCTGGGG TGGCTATGTC CTGTACCCTC AGGTGATGGA GTTCTGGCAA 
GGTCAAACCA ACCGCCTGCA TGACCGGATA GTCTTTCGGC GGGGCCTACC CACAGGAGAT 
TCCCCTTTGG GGCCCATGAC CCACCGCGGG GAGGAAGACT GGCTCTATGA GAGACTTGCA 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPO ( XM_017024813.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880302.1 Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu50514
Clone ID Related Accession (Same CDS sequence)	XM_017024813.1 , XM_005257500.3
Accession Version	XM_017024813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyridoxine-5'-phosphate oxidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCTGG CTACCTGCAC CAGAGATGGA AAACCCTCTG CTCGCATGTT GCTGCTGAAG 
GGCTTCGGGA AAGATGGCTT CCGCTTCTTC ACTAACTTCG AGAGTCGAAA AGGAAAAGAG 
CTGGACTCTA ATCCCTTTGC TTCCCTTGTC TTCTACTGGG AGCCACTTAA CCGTCAGGTG 
CGTGTGGAAG GCCCTGTGAA GAAACTGCCT GAGGAGGAGG CTGAGTGCTA CTTCCACTCC 
CGCCCCAAGA GCAGCCAGAT TGGGGCTGTG GTCAGCCACC AGAGTTCTGT GATCCCTGAT 
CGGGAGTATC TGAGAAAGAA AAATGAGGAA CTGGAACAGC TCTACCAGGA TCAAGAGGTG 
CCCAAGCCAA AATCCTGGGG TGGCTATGTC CTGTACCCTC AGGTGATGGA GTTCTGGCAA 
GGTCAAACCA ACCGCCTGCA TGACCGGATA GTCTTTCGGC GGGGCCTACC CACAGGAGAT 
TCCCCTTTGG GGCCCATGAC CCACCGCGGG GAGGAAGACT GGCTCTATGA GAGACTTGCA 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880302.1
CDS	182..727
Translation	MCLATCTRDGKPSARMLLLKGFGKDGFRFFTNFESRKGKELDSNPFASLVFYWEPLNRQVRVEGPVKKLPEEEAECYFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYVLYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP

Target ORF information:

RefSeq Version	XM_017024813.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024813.1

ORF Insert Sequence:

ATGTGTCTGG CTACCTGCAC CAGAGATGGA AAACCCTCTG CTCGCATGTT GCTGCTGAAG 
GGCTTCGGGA AAGATGGCTT CCGCTTCTTC ACTAACTTCG AGAGTCGAAA AGGAAAAGAG 
CTGGACTCTA ATCCCTTTGC TTCCCTTGTC TTCTACTGGG AGCCACTTAA CCGTCAGGTG 
CGTGTGGAAG GCCCTGTGAA GAAACTGCCT GAGGAGGAGG CTGAGTGCTA CTTCCACTCC 
CGCCCCAAGA GCAGCCAGAT TGGGGCTGTG GTCAGCCACC AGAGTTCTGT GATCCCTGAT 
CGGGAGTATC TGAGAAAGAA AAATGAGGAA CTGGAACAGC TCTACCAGGA TCAAGAGGTG 
CCCAAGCCAA AATCCTGGGG TGGCTATGTC CTGTACCCTC AGGTGATGGA GTTCTGGCAA 
GGTCAAACCA ACCGCCTGCA TGACCGGATA GTCTTTCGGC GGGGCCTACC CACAGGAGAT 
TCCCCTTTGG GGCCCATGAC CCACCGCGGG GAGGAAGACT GGCTCTATGA GAGACTTGCA 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPO ( XM_005257500.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005257557.1 Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu50514
Clone ID Related Accession (Same CDS sequence)	XM_017024813.1 , XM_005257500.3
Accession Version	XM_005257500.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyridoxine-5'-phosphate oxidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257500.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCTGG CTACCTGCAC CAGAGATGGA AAACCCTCTG CTCGCATGTT GCTGCTGAAG 
GGCTTCGGGA AAGATGGCTT CCGCTTCTTC ACTAACTTCG AGAGTCGAAA AGGAAAAGAG 
CTGGACTCTA ATCCCTTTGC TTCCCTTGTC TTCTACTGGG AGCCACTTAA CCGTCAGGTG 
CGTGTGGAAG GCCCTGTGAA GAAACTGCCT GAGGAGGAGG CTGAGTGCTA CTTCCACTCC 
CGCCCCAAGA GCAGCCAGAT TGGGGCTGTG GTCAGCCACC AGAGTTCTGT GATCCCTGAT 
CGGGAGTATC TGAGAAAGAA AAATGAGGAA CTGGAACAGC TCTACCAGGA TCAAGAGGTG 
CCCAAGCCAA AATCCTGGGG TGGCTATGTC CTGTACCCTC AGGTGATGGA GTTCTGGCAA 
GGTCAAACCA ACCGCCTGCA TGACCGGATA GTCTTTCGGC GGGGCCTACC CACAGGAGAT 
TCCCCTTTGG GGCCCATGAC CCACCGCGGG GAGGAAGACT GGCTCTATGA GAGACTTGCA 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005257557.1
CDS	584..1129
Translation	MCLATCTRDGKPSARMLLLKGFGKDGFRFFTNFESRKGKELDSNPFASLVFYWEPLNRQVRVEGPVKKLPEEEAECYFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYVLYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP

Target ORF information:

RefSeq Version	XM_005257500.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257500.3

ORF Insert Sequence:

ATGTGTCTGG CTACCTGCAC CAGAGATGGA AAACCCTCTG CTCGCATGTT GCTGCTGAAG 
GGCTTCGGGA AAGATGGCTT CCGCTTCTTC ACTAACTTCG AGAGTCGAAA AGGAAAAGAG 
CTGGACTCTA ATCCCTTTGC TTCCCTTGTC TTCTACTGGG AGCCACTTAA CCGTCAGGTG 
CGTGTGGAAG GCCCTGTGAA GAAACTGCCT GAGGAGGAGG CTGAGTGCTA CTTCCACTCC 
CGCCCCAAGA GCAGCCAGAT TGGGGCTGTG GTCAGCCACC AGAGTTCTGT GATCCCTGAT 
CGGGAGTATC TGAGAAAGAA AAATGAGGAA CTGGAACAGC TCTACCAGGA TCAAGAGGTG 
CCCAAGCCAA AATCCTGGGG TGGCTATGTC CTGTACCCTC AGGTGATGGA GTTCTGGCAA 
GGTCAAACCA ACCGCCTGCA TGACCGGATA GTCTTTCGGC GGGGCCTACC CACAGGAGAT 
TCCCCTTTGG GGCCCATGAC CCACCGCGGG GAGGAAGACT GGCTCTATGA GAGACTTGCA 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNPO ( XM_011524968.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523270.1 Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), transcript variant X3, mRNA.

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CloneID	OHu69098
Clone ID Related Accession (Same CDS sequence)	XM_011524968.2
Accession Version	XM_011524968.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyridoxine-5'-phosphate oxidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTGC TGAAGGGCTT CGGGAAAGAT GGCTTCCGCT TCTTCACTAA CTTCGAGAGT 
CGAAAAGGAA AAGAGCTGGA CTCTAATCCC TTTGCTTCCC TTGTCTTCTA CTGGGAGCCA 
CTTAACCGTC AGGTGCGTGT GGAAGGCCCT GTGAAGAAAC TGCCTGAGGA GGAGGCTGAG 
TGCTACTTCC ACTCCCGCCC CAAGAGCAGC CAGATTGGGG CTGTGGTCAG CCACCAGAGT 
TCTGTGATCC CTGATCGGGA GTATCTGAGA AAGAAAAATG AGGAACTGGA ACAGCTCTAC 
CAGGATCAAG AGGTGCCCAA GCCAAAATCC TGGGGTGGCT ATGTCCTGTA CCCTCAGGTG 
ATGGAGTTCT GGCAAGGTCA AACCAACCGC CTGCATGACC GGATAGTCTT TCGGCGGGGC 
CTACCCACAG GAGATTCCCC TTTGGGGCCC ATGACCCACC GCGGGGAGGA AGACTGGCTC 
TATGAGAGAC TTGCACCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523270.1
CDS	212..712
Translation	MLLLKGFGKDGFRFFTNFESRKGKELDSNPFASLVFYWEPLNRQVRVEGPVKKLPEEEAECYFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYVLYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP

Target ORF information:

RefSeq Version	XM_011524968.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pyridoxamine 5'-phosphate oxidase (PNPO), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011524968.2

ORF Insert Sequence:

ATGTTGCTGC TGAAGGGCTT CGGGAAAGAT GGCTTCCGCT TCTTCACTAA CTTCGAGAGT 
CGAAAAGGAA AAGAGCTGGA CTCTAATCCC TTTGCTTCCC TTGTCTTCTA CTGGGAGCCA 
CTTAACCGTC AGGTGCGTGT GGAAGGCCCT GTGAAGAAAC TGCCTGAGGA GGAGGCTGAG 
TGCTACTTCC ACTCCCGCCC CAAGAGCAGC CAGATTGGGG CTGTGGTCAG CCACCAGAGT 
TCTGTGATCC CTGATCGGGA GTATCTGAGA AAGAAAAATG AGGAACTGGA ACAGCTCTAC 
CAGGATCAAG AGGTGCCCAA GCCAAAATCC TGGGGTGGCT ATGTCCTGTA CCCTCAGGTG 
ATGGAGTTCT GGCAAGGTCA AACCAACCGC CTGCATGACC GGATAGTCTT TCGGCGGGGC 
CTACCCACAG GAGATTCCCC TTTGGGGCCC ATGACCCACC GCGGGGAGGA AGACTGGCTC 
TATGAGAGAC TTGCACCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pyridoxine 5'-phosphate oxidase is a novel therapeutic target and regulated by the TGF-? signalling pathway in epithelial ovarian cancer.
Cell death & disease8(12)3214(2017 Dec)
Zhang L,Zhou D,Guan W,Ren W,Sun W,Shi J,Lin Q,Zhang J,Qiao T,Ye Y,Wu Y,Zhang Y,Zuo X,Connor KL,Xu G

Pyridoxine-5'-phosphate oxidase (Pnpo) deficiency: Clinical and biochemical alterations associated with the C.347g>A (P.?Arg116gln) mutation.
Molecular genetics and metabolism122(1-2)135-142(2017 Sep)
di Salvo ML,Mastrangelo M,Nogu?s I,Tolve M,Paiardini A,Carducci C,Mei D,Montomoli M,Tramonti A,Guerrini R,Contestabile R,Leuzzi V

Pyridoxal 5?-phosphate-responsive epilepsy with novel mutations in the PNPO gene: a case report.
Genetics and molecular research : GMR14(4)14130-5(2015 Oct)
Veeravigrom M,Damrongphol P,Ittiwut R,Ittiwut C,Suphapeetiporn K,Shotelersuk V

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kov?cs IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barab?si AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

Pyridoxine responsiveness in novel mutations of the PNPO gene.
Neurology82(16)1425-33(2014 Apr)
Plecko B,Paul K,Mills P,Clayton P,Paschke E,Maier O,Hasselmann O,Schmiedel G,Kanz S,Connolly M,Wolf N,Struys E,Stockler S,Abela L,Hofer D

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PNPO cDNA ORF clone, Homo sapiens(human)

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Clones

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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