ANKHD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKHD1
Entrez Gene ID	54882
Full Name	ankyrin repeat and KH domain containing 1
Synonyms	MASK,MASK1,PP2500,VBARP
General protein information	Preferred Names ankyrin repeat and KH domain containing 1 Names ankyrin repeat and KH domain-containing protein 1 HIV-1 Vpr-binding ankyrin repeat protein multiple ankyrin repeats, single KH-domain homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.3
Summary	This gene encodes a protein with multiple ankyrin repeat domains and a single KH-domain. The protein is thought to function as a scaffolding protein, and it may be involved in the regulation of caspases and thereby play an antiapoptotic role in cell survival. Alternative splicing results in multiple transcript variants, one of which generates a fusion transcript (MASK-BP3) with the downstream eIF4E-binding protein 3 (EIF4EBP3) gene, resulting in a protein comprised of the ANKHD1 sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. [provided by RefSeq, Sep 2010].
Disorder MIM:	610500

mRNA and Protein(s)

mRNA	Protein	Name
NM_017747.2	NP_060217.1	ankyrin repeat and KH domain-containing protein 1 isoform 1
NM_017978.2	NP_060448.1	ankyrin repeat and KH domain-containing protein 1 isoform 2
NM_024668.3	NP_078944.2	ankyrin repeat and KH domain-containing protein 1 isoform 3
NM_017978.3	NP_060448.1	ankyrin repeat and KH domain-containing protein 1 isoform 2
NM_024668.4	NP_078944.2	ankyrin repeat and KH domain-containing protein 1 isoform 3
NM_017747.3	NP_060217.1	ankyrin repeat and KH domain-containing protein 1 isoform 1
NM_001197030.1	NP_001183959.1	ankyrin repeat and KH domain-containing protein 1 isoform 4

Pathways from BioSystems

Homologies

Homo sapiens (human)	ANKHD1-EIF4EBP3	NP_065741.3
Canis lupus familiaris (dog)	ANKHD1	NP_001191024.1
Mus musculus (house mouse)	Ankhd1	NP_780584.2
Rattus norvegicus (Norway rat)	Ankhd1	NP_001190982.1
Homo sapiens (human)	ANKHD1	NP_060217.1
Xenopus tropicalis (tropical clawed frog)	ankhd1	NP_001191021.1
Bos taurus (cattle)	ANKHD1	XP_005209554.1
Danio rerio (zebrafish)	ankhd1	NP_001186697.1
	ANKHD1	XP_002804594.1
Gallus gallus (chicken)	ANKHD1	NP_001191026.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization and Genetic Analyses of New Genes Coding for NOD2 Interacting Proteins.
Thi?baut R, Esmiol S, Lecine P, Mahfouz B, Hermant A, Nicoletti C, Parnis S, Perroy J, Borg JP, Pascoe L, Hugot JP, Ollendorff V
PloS one11(11)e0165420(2016)

ANKHD1 silencing inhibits Stathmin 1 activity, cell proliferation and migration of leukemia cells.
Machado-Neto JA, Lazarini M, Favaro P, de Melo Campos P, Scopim-Ribeiro R, Franchi Junior GC, Nowill AE, Lima PR, Costa FF, Benichou S, Olalla Saad ST, Traina F
Biochimica et biophysica acta1853(3)583-93(2015 Mar)

ANKHD1 represses p21 (WAF1/CIP1) promoter and promotes multiple myeloma cell growth.
Dhyani A, Machado-Neto JA, Favaro P, Saad ST
European journal of cancer (Oxford, England : 1990)51(2)252-9(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ANKHD1 may be an oncogene and participate in the leukemia cell phenotype.
Title: ANKHD1 silencing inhibits Stathmin 1 activity, cell proliferation and migration of leukemia cells.

Data suggest an association of ANKHD1 (Ankyrin repeat and KH domain-containing protein 1) protein with p21 (WAF1/CIP1) promoter region.
Title: ANKHD1 represses p21 (WAF1/CIP1) promoter and promotes multiple myeloma cell growth.

ANKHD1 is a positive regulator of YAP1 and promotes cell growth and cell cycle progression through Cyclin A upregulation.
Title: ANKHD1, a novel component of the Hippo signaling pathway, promotes YAP1 activation and cell cycle progression in prostate cancer cells.

These data suggest that ANKHD1 might have a role in multiple myeloma cell proliferation and cell cycle progression by regulating expression of p21.
Title: ANKHD1 regulates cell cycle progression and proliferation in multiple myeloma cells.

These findings suggest a role for ANKHD1 as a scaffolding protein that may be associated with the abnormal phenotype of leukemia cells.
Title: ANKHD1, ankyrin repeat and KH domain containing 1, is overexpressed in acute leukemias and is associated with SHP2 in K562 cells.

The following ANKHD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKHD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02639	NM_017978.3 Latest version!	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$259.50-$363.30 $519.00
OHu04164	NM_024668.4 Latest version!	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu05657	NM_001197030.1 Latest version!	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OHu02533	NM_017747.3 Latest version!	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
View Old Accession Versions >>
OHu02639	NM_017978.2	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$259.50-$363.30 $519.00
OHu04164	NM_024668.3	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu02533	NM_017747.2	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
Hide Old Accession Versions >>

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Reference Sequences (Refseq)

ANKHD1 ( NM_017978.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060448.1 Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02639
Clone ID Related Accession (Same CDS sequence)	NM_017978.2 , NM_017978.3
Accession Version	NM_017978.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Homo sapiens(human)
Product	ankyrin repeat and KH domain-containing protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA509209.1 and BC009420.1. On Oct 1, 2010 this sequence version replaced NM_017978.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, lacks several exons in the central and 3' coding regions, and uses an alternate 3' terminal exon, compared to variant 1. The resulting isoform (2) is significantly shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG CTTTTGCAGA TCCTGAGGTA 
CTCCGGAGAC TGACATCCTC AGTTAGTTGT GCACTGGATG AAGCTGCTGC TGCACTGACA 
CGGATGAAAG CAGAAAACAG CCACAATGCA GGACAAGTGG ACACTCGCAG TCTAGCAGAA 
GCTTGTTCAG ATGGGGATGT TAATGCTGTT CGTAAATTGC TAGATGAAGG CAGAAGTGTA 
AATGAACATA CAGAAGAAGG AGAAAGCCTG CTGTGTTTGG CTTGTTCAGC AGGGTATTAT 
GAATTAGCAC AAGTATTGCT TGCTATGCAT GCTAATGTTG AAGATCGAGG GAATAAAGGA 
GACATAACTC CCCTGATGGC AGCTTCCAGT GGAGGTTACT TAGATATTGT GAAATTATTA 
CTTCTTCATG ATGCTGATGT CAACTCCCAG TCTGCAACAG GAAACACTGC GCTAACTTAT 
GCATGTGCTG GAGGATTTGT TGACATTGTT AAAGTGCTCC TTAATGAAGG TGCAAATATA 
GAAGATCATA ATGAAAATGG ACATACTCCC TTAATGGAAG CAGCCAGTGC AGGTCATGTG 
GAAGTTGCAA GAGTTCTTTT AGATCATGGT GCAGGCATCA ACACTCATTC TAATGAATTC 
AAAGAAAGTG CTCTAACACT TGCTTGCTAC AAAGGCCATT TGGATATGGT TCGCTTTCTA 
CTTGAAGCTG GTGCAGATCA AGAGCACAAA ACAGATGAGA TGCACACTGC CTTAATGGAG 
GCCTGCATGG ATGGACATGT AGAGGTGGCA CGTTTGCTTT TGGATAGTGG TGCTCAAGTG 
AACATGCCTG CAGATTCATT TGAATCTCCA TTGACGCTAG CTGCCTGTGG AGGACATGTT 
GAATTGGCAG CTCTACTTAT TGAAAGGGGA GCAAATCTTG AAGAAGTTAA TGATGAAGGA 
TACACTCCCT TGATGGAAGC TGCCCGGGAA GGACATGAAG AAATGGTGGC ACTACTCTTA 
GCACAAGGAG CAAATATAAA TGCCCAGACA GAAGAAACTC AAGAAACTGC TCTTACTTTG 
GCTTGCTGTG GAGGATTTTC TGAAGTTGCA GACTTTCTTA TTAAGGCAGG GGCTGATATA 
GAACTTGGCT GCTCCACACC TCTGATGGAG GCATCTCAGG AGGGACACCT GGAATTGGTT 
AAATATTTGC TGGCTTCTGG CGCTAATGTG CATGCTACAA CAGCAACAGG AGACACAGCC 
TTAACCTATG CTTGTGAAAA TGGACATACG GATGTTGCAG ATGTTTTACT TCAAGCAGGG 
GCTGATTTAG ACAAGCAGGA GGACATGAAG ACTATTTTGG AGGGCATAGA TCCGGCCAAG 
CATCAGGTGA GGGTGGCCTT TGATGCTTGT AAGCTACTAC GTAAAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060448.1
CDS	155..2005
Translation	MLTDSGGGGTSFEEDLDSVAPRSAPAGASEPPPPGGVGLGIRTVRLFGEAGPASGVGSSGGGGSGSGTGGGDAALDFKLAAAVLRTGGGGGASGSDEDEVSEVESFILDQEDLDNPVLKTTSEIFLSSTAEGADLRTVDPETQARLEALLEAAAFADPEVLRRLTSSVSCALDEAAAALTRMKAENSHNAGQVDTRSLAEACSDGDVNAVRKLLDEGRSVNEHTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGNKGDITPLMAASSGGYLDIVKLLLLHDADVNSQSATGNTALTYACAGGFVDIVKVLLNEGANIEDHNENGHTPLMEAASAGHVEVARVLLDHGAGINTHSNEFKESALTLACYKGHLDMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFSEVADFLIKAGADIELGCSTPLMEASQEGHLELVKYLLASGANVHATTATGDTALTYACENGHTDVADVLLQAGADLDKQEDMKTILEGIDPAKHQVRVAFDACKLLRKE

Target ORF information:

RefSeq Version	NM_017978.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017978.2

ORF Insert Sequence:

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG CTTTTGCAGA TCCTGAGGTA 
CTCCGGAGAC TGACATCCTC AGTTAGTTGT GCACTGGATG AAGCTGCTGC TGCACTGACA 
CGGATGAAAG CAGAAAACAG CCACAATGCA GGACAAGTGG ACACTCGCAG TCTAGCAGAA 
GCTTGTTCAG ATGGGGATGT TAATGCTGTT CGTAAATTGC TAGATGAAGG CAGAAGTGTA 
AATGAACATA CAGAAGAAGG AGAAAGCCTG CTGTGTTTGG CTTGTTCAGC AGGGTATTAT 
GAATTAGCAC AAGTATTGCT TGCTATGCAT GCTAATGTTG AAGATCGAGG GAATAAAGGA 
GACATAACTC CCCTGATGGC AGCTTCCAGT GGAGGTTACT TAGATATTGT GAAATTATTA 
CTTCTTCATG ATGCTGATGT CAACTCCCAG TCTGCAACAG GAAACACTGC GCTAACTTAT 
GCATGTGCTG GAGGATTTGT TGACATTGTT AAAGTGCTCC TTAATGAAGG TGCAAATATA 
GAAGATCATA ATGAAAATGG ACATACTCCC TTAATGGAAG CAGCCAGTGC AGGTCATGTG 
GAAGTTGCAA GAGTTCTTTT AGATCATGGT GCAGGCATCA ACACTCATTC TAATGAATTC 
AAAGAAAGTG CTCTAACACT TGCTTGCTAC AAAGGCCATT TGGATATGGT TCGCTTTCTA 
CTTGAAGCTG GTGCAGATCA AGAGCACAAA ACAGATGAGA TGCACACTGC CTTAATGGAG 
GCCTGCATGG ATGGACATGT AGAGGTGGCA CGTTTGCTTT TGGATAGTGG TGCTCAAGTG 
AACATGCCTG CAGATTCATT TGAATCTCCA TTGACGCTAG CTGCCTGTGG AGGACATGTT 
GAATTGGCAG CTCTACTTAT TGAAAGGGGA GCAAATCTTG AAGAAGTTAA TGATGAAGGA 
TACACTCCCT TGATGGAAGC TGCCCGGGAA GGACATGAAG AAATGGTGGC ACTACTCTTA 
GCACAAGGAG CAAATATAAA TGCCCAGACA GAAGAAACTC AAGAAACTGC TCTTACTTTG 
GCTTGCTGTG GAGGATTTTC TGAAGTTGCA GACTTTCTTA TTAAGGCAGG GGCTGATATA 
GAACTTGGCT GCTCCACACC TCTGATGGAG GCATCTCAGG AGGGACACCT GGAATTGGTT 
AAATATTTGC TGGCTTCTGG CGCTAATGTG CATGCTACAA CAGCAACAGG AGACACAGCC 
TTAACCTATG CTTGTGAAAA TGGACATACG GATGTTGCAG ATGTTTTACT TCAAGCAGGG 
GCTGATTTAG ACAAGCAGGA GGACATGAAG ACTATTTTGG AGGGCATAGA TCCGGCCAAG 
CATCAGGTGA GGGTGGCCTT TGATGCTTGT AAGCTACTAC GTAAAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKHD1 ( NM_017978.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060448.1 Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02639
Clone ID Related Accession (Same CDS sequence)	NM_017978.2 , NM_017978.3
Accession Version	NM_017978.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	ankyrin repeat and KH domain-containing protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA509209.1 and BC009420.1. On May 31, 2019 this sequence version replaced NM_017978.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, lacks several exons in the central and 3' coding regions, and uses an alternate 3' terminal exon, compared to variant 1. The resulting isoform (2) is significantly shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG CTTTTGCAGA TCCTGAGGTA 
CTCCGGAGAC TGACATCCTC AGTTAGTTGT GCACTGGATG AAGCTGCTGC TGCACTGACA 
CGGATGAAAG CAGAAAACAG CCACAATGCA GGACAAGTGG ACACTCGCAG TCTAGCAGAA 
GCTTGTTCAG ATGGGGATGT TAATGCTGTT CGTAAATTGC TAGATGAAGG CAGAAGTGTA 
AATGAACATA CAGAAGAAGG AGAAAGCCTG CTGTGTTTGG CTTGTTCAGC AGGGTATTAT 
GAATTAGCAC AAGTATTGCT TGCTATGCAT GCTAATGTTG AAGATCGAGG GAATAAAGGA 
GACATAACTC CCCTGATGGC AGCTTCCAGT GGAGGTTACT TAGATATTGT GAAATTATTA 
CTTCTTCATG ATGCTGATGT CAACTCCCAG TCTGCAACAG GAAACACTGC GCTAACTTAT 
GCATGTGCTG GAGGATTTGT TGACATTGTT AAAGTGCTCC TTAATGAAGG TGCAAATATA 
GAAGATCATA ATGAAAATGG ACATACTCCC TTAATGGAAG CAGCCAGTGC AGGTCATGTG 
GAAGTTGCAA GAGTTCTTTT AGATCATGGT GCAGGCATCA ACACTCATTC TAATGAATTC 
AAAGAAAGTG CTCTAACACT TGCTTGCTAC AAAGGCCATT TGGATATGGT TCGCTTTCTA 
CTTGAAGCTG GTGCAGATCA AGAGCACAAA ACAGATGAGA TGCACACTGC CTTAATGGAG 
GCCTGCATGG ATGGACATGT AGAGGTGGCA CGTTTGCTTT TGGATAGTGG TGCTCAAGTG 
AACATGCCTG CAGATTCATT TGAATCTCCA TTGACGCTAG CTGCCTGTGG AGGACATGTT 
GAATTGGCAG CTCTACTTAT TGAAAGGGGA GCAAATCTTG AAGAAGTTAA TGATGAAGGA 
TACACTCCCT TGATGGAAGC TGCCCGGGAA GGACATGAAG AAATGGTGGC ACTACTCTTA 
GCACAAGGAG CAAATATAAA TGCCCAGACA GAAGAAACTC AAGAAACTGC TCTTACTTTG 
GCTTGCTGTG GAGGATTTTC TGAAGTTGCA GACTTTCTTA TTAAGGCAGG GGCTGATATA 
GAACTTGGCT GCTCCACACC TCTGATGGAG GCATCTCAGG AGGGACACCT GGAATTGGTT 
AAATATTTGC TGGCTTCTGG CGCTAATGTG CATGCTACAA CAGCAACAGG AGACACAGCC 
TTAACCTATG CTTGTGAAAA TGGACATACG GATGTTGCAG ATGTTTTACT TCAAGCAGGG 
GCTGATTTAG ACAAGCAGGA GGACATGAAG ACTATTTTGG AGGGCATAGA TCCGGCCAAG 
CATCAGGTGA GGGTGGCCTT TGATGCTTGT AAGCTACTAC GTAAAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060448.1
CDS	136..1986
Translation	MLTDSGGGGTSFEEDLDSVAPRSAPAGASEPPPPGGVGLGIRTVRLFGEAGPASGVGSSGGGGSGSGTGGGDAALDFKLAAAVLRTGGGGGASGSDEDEVSEVESFILDQEDLDNPVLKTTSEIFLSSTAEGADLRTVDPETQARLEALLEAAAFADPEVLRRLTSSVSCALDEAAAALTRMKAENSHNAGQVDTRSLAEACSDGDVNAVRKLLDEGRSVNEHTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGNKGDITPLMAASSGGYLDIVKLLLLHDADVNSQSATGNTALTYACAGGFVDIVKVLLNEGANIEDHNENGHTPLMEAASAGHVEVARVLLDHGAGINTHSNEFKESALTLACYKGHLDMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFSEVADFLIKAGADIELGCSTPLMEASQEGHLELVKYLLASGANVHATTATGDTALTYACENGHTDVADVLLQAGADLDKQEDMKTILEGIDPAKHQVRVAFDACKLLRKE

Target ORF information:

RefSeq Version	NM_017978.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017978.3

ORF Insert Sequence:

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG CTTTTGCAGA TCCTGAGGTA 
CTCCGGAGAC TGACATCCTC AGTTAGTTGT GCACTGGATG AAGCTGCTGC TGCACTGACA 
CGGATGAAAG CAGAAAACAG CCACAATGCA GGACAAGTGG ACACTCGCAG TCTAGCAGAA 
GCTTGTTCAG ATGGGGATGT TAATGCTGTT CGTAAATTGC TAGATGAAGG CAGAAGTGTA 
AATGAACATA CAGAAGAAGG AGAAAGCCTG CTGTGTTTGG CTTGTTCAGC AGGGTATTAT 
GAATTAGCAC AAGTATTGCT TGCTATGCAT GCTAATGTTG AAGATCGAGG GAATAAAGGA 
GACATAACTC CCCTGATGGC AGCTTCCAGT GGAGGTTACT TAGATATTGT GAAATTATTA 
CTTCTTCATG ATGCTGATGT CAACTCCCAG TCTGCAACAG GAAACACTGC GCTAACTTAT 
GCATGTGCTG GAGGATTTGT TGACATTGTT AAAGTGCTCC TTAATGAAGG TGCAAATATA 
GAAGATCATA ATGAAAATGG ACATACTCCC TTAATGGAAG CAGCCAGTGC AGGTCATGTG 
GAAGTTGCAA GAGTTCTTTT AGATCATGGT GCAGGCATCA ACACTCATTC TAATGAATTC 
AAAGAAAGTG CTCTAACACT TGCTTGCTAC AAAGGCCATT TGGATATGGT TCGCTTTCTA 
CTTGAAGCTG GTGCAGATCA AGAGCACAAA ACAGATGAGA TGCACACTGC CTTAATGGAG 
GCCTGCATGG ATGGACATGT AGAGGTGGCA CGTTTGCTTT TGGATAGTGG TGCTCAAGTG 
AACATGCCTG CAGATTCATT TGAATCTCCA TTGACGCTAG CTGCCTGTGG AGGACATGTT 
GAATTGGCAG CTCTACTTAT TGAAAGGGGA GCAAATCTTG AAGAAGTTAA TGATGAAGGA 
TACACTCCCT TGATGGAAGC TGCCCGGGAA GGACATGAAG AAATGGTGGC ACTACTCTTA 
GCACAAGGAG CAAATATAAA TGCCCAGACA GAAGAAACTC AAGAAACTGC TCTTACTTTG 
GCTTGCTGTG GAGGATTTTC TGAAGTTGCA GACTTTCTTA TTAAGGCAGG GGCTGATATA 
GAACTTGGCT GCTCCACACC TCTGATGGAG GCATCTCAGG AGGGACACCT GGAATTGGTT 
AAATATTTGC TGGCTTCTGG CGCTAATGTG CATGCTACAA CAGCAACAGG AGACACAGCC 
TTAACCTATG CTTGTGAAAA TGGACATACG GATGTTGCAG ATGTTTTACT TCAAGCAGGG 
GCTGATTTAG ACAAGCAGGA GGACATGAAG ACTATTTTGG AGGGCATAGA TCCGGCCAAG 
CATCAGGTGA GGGTGGCCTT TGATGCTTGT AAGCTACTAC GTAAAGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKHD1 ( NM_024668.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_078944.2 Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 3, mRNA.

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CloneID	OHu04164
Clone ID Related Accession (Same CDS sequence)	NM_024668.3 , NM_024668.4
Accession Version	NM_024668.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Homo sapiens(human)
Product	ankyrin repeat and KH domain-containing protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA509209.1 and BC004457.1. On Oct 1, 2010 this sequence version replaced NM_024668.2. Transcript Variant: This variant (3) lacks several exons in the central and 3' coding regions, and uses an alternate 3' terminal exon, compared to variant 1. The resulting isoform (3) is significantly shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.336298.1, SRR1660803.76114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG GAATTGGCAA ATTGTCAACT 
GCTGATGGTA AAGCTTTTGC AGATCCTGAG GTACTCCGGA GACTGACATC CTCAGTTAGT 
TGTGCACTGG ATGAAGCTGC TGCTGCACTG ACACGGATGA AAGCAGAAAA CAGCCACAAT 
GCAGGACAAG TGGACACTCG CAGTCTAGCA GAAGCTTGTT CAGATGGGGA TGTTAATGCT 
GTTCGTAAAT TGCTAGATGA AGGCAGAAGT GTAAATGAAC ATACAGAAGA AGGAGAAAGC 
CTGCTGTGTT TGGCTTGTTC AGCAGGGTAT TATGAATTAG CACAAGTATT GCTTGCTATG 
CATGCTAATG TTGAAGATCG AGGGAATAAA GGAGACATAA CTCCCCTGAT GGCAGCTTCC 
AGTGGAGGTT ACTTAGATAT TGTGAAATTA TTACTTCTTC ATGATGCTGA TGTCAACTCC 
CAGTCTGCAA CAGGAAACAC TGCGCTAACT TATGCATGTG CTGGAGGATT TGTTGACATT 
GTTAAAGTGC TCCTTAATGA AGGTGCAAAT ATAGAAGATC ATAATGAAAA TGGACATACT 
CCCTTAATGG AAGCAGCCAG TGCAGGTCAT GTGGAAGTTG CAAGAGTTCT TTTAGATCAT 
GGTGCAGGCA TCAACACTCA TTCTAATGAA TTCAAAGAAA GTGCTCTAAC ACTTGCTTGC 
TACAAAGGCC ATTTGGATAT GGTTCGCTTT CTACTTGAAG CTGGTGCAGA TCAAGAGCAC 
AAAACAGATG AGATGCACAC TGCCTTAATG GAGGCCTGCA TGGATGGACA TGTAGAGGTG 
GCACGTTTGC TTTTGGATAG TGGTGCTCAA GTGAACATGC CTGCAGATTC ATTTGAATCT 
CCATTGACGC TAGCTGCCTG TGGAGGACAT GTTGAATTGG CAGCTCTACT TATTGAAAGG 
GGAGCAAATC TTGAAGAAGT TAATGATGAA GGATACACTC CCTTGATGGA AGCTGCCCGG 
GAAGGACATG AAGAAATGGT GGCACTACTC TTAGCACAAG GAGCAAATAT AAATGCCCAG 
ACAGAAGAAA CTCAAGAAAC TGCTCTTACT TTGGCTTGCT GTGGAGGATT TTCTGAAGTT 
GCAGACTTTC TTATTAAGGC AGGGGCTGAT ATAGAACTTG GCTGCTCCAC ACCTCTGATG 
GAGGCATCTC AGGAGGGACA CCTGGAATTG GTTAAATATT TGCTGGCTTC TGGCGCTAAT 
GTGCATGCTA CAACAGCAAC AGGAGACACA GCCTTAACCT ATGCTTGTGA AAATGGACAT 
ACGGATGTTG CAGATGTTTT ACTTCAAGCA GGGGCTGATT TAGACAAGCA GGAGGACATG 
AAGACTATTT TGGAGGGCAT AGATCCGGCC AAGCATCAGG TGAGGGTGGC CTTTGATGCT 
TGTAAGCTAC TACGTAAAGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078944.2
CDS	155..2038
Translation	MLTDSGGGGTSFEEDLDSVAPRSAPAGASEPPPPGGVGLGIRTVRLFGEAGPASGVGSSGGGGSGSGTGGGDAALDFKLAAAVLRTGGGGGASGSDEDEVSEVESFILDQEDLDNPVLKTTSEIFLSSTAEGADLRTVDPETQARLEALLEAAGIGKLSTADGKAFADPEVLRRLTSSVSCALDEAAAALTRMKAENSHNAGQVDTRSLAEACSDGDVNAVRKLLDEGRSVNEHTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGNKGDITPLMAASSGGYLDIVKLLLLHDADVNSQSATGNTALTYACAGGFVDIVKVLLNEGANIEDHNENGHTPLMEAASAGHVEVARVLLDHGAGINTHSNEFKESALTLACYKGHLDMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFSEVADFLIKAGADIELGCSTPLMEASQEGHLELVKYLLASGANVHATTATGDTALTYACENGHTDVADVLLQAGADLDKQEDMKTILEGIDPAKHQVRVAFDACKLLRKE

Target ORF information:

RefSeq Version	NM_024668.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024668.3

ORF Insert Sequence:

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG GAATTGGCAA ATTGTCAACT 
GCTGATGGTA AAGCTTTTGC AGATCCTGAG GTACTCCGGA GACTGACATC CTCAGTTAGT 
TGTGCACTGG ATGAAGCTGC TGCTGCACTG ACACGGATGA AAGCAGAAAA CAGCCACAAT 
GCAGGACAAG TGGACACTCG CAGTCTAGCA GAAGCTTGTT CAGATGGGGA TGTTAATGCT 
GTTCGTAAAT TGCTAGATGA AGGCAGAAGT GTAAATGAAC ATACAGAAGA AGGAGAAAGC 
CTGCTGTGTT TGGCTTGTTC AGCAGGGTAT TATGAATTAG CACAAGTATT GCTTGCTATG 
CATGCTAATG TTGAAGATCG AGGGAATAAA GGAGACATAA CTCCCCTGAT GGCAGCTTCC 
AGTGGAGGTT ACTTAGATAT TGTGAAATTA TTACTTCTTC ATGATGCTGA TGTCAACTCC 
CAGTCTGCAA CAGGAAACAC TGCGCTAACT TATGCATGTG CTGGAGGATT TGTTGACATT 
GTTAAAGTGC TCCTTAATGA AGGTGCAAAT ATAGAAGATC ATAATGAAAA TGGACATACT 
CCCTTAATGG AAGCAGCCAG TGCAGGTCAT GTGGAAGTTG CAAGAGTTCT TTTAGATCAT 
GGTGCAGGCA TCAACACTCA TTCTAATGAA TTCAAAGAAA GTGCTCTAAC ACTTGCTTGC 
TACAAAGGCC ATTTGGATAT GGTTCGCTTT CTACTTGAAG CTGGTGCAGA TCAAGAGCAC 
AAAACAGATG AGATGCACAC TGCCTTAATG GAGGCCTGCA TGGATGGACA TGTAGAGGTG 
GCACGTTTGC TTTTGGATAG TGGTGCTCAA GTGAACATGC CTGCAGATTC ATTTGAATCT 
CCATTGACGC TAGCTGCCTG TGGAGGACAT GTTGAATTGG CAGCTCTACT TATTGAAAGG 
GGAGCAAATC TTGAAGAAGT TAATGATGAA GGATACACTC CCTTGATGGA AGCTGCCCGG 
GAAGGACATG AAGAAATGGT GGCACTACTC TTAGCACAAG GAGCAAATAT AAATGCCCAG 
ACAGAAGAAA CTCAAGAAAC TGCTCTTACT TTGGCTTGCT GTGGAGGATT TTCTGAAGTT 
GCAGACTTTC TTATTAAGGC AGGGGCTGAT ATAGAACTTG GCTGCTCCAC ACCTCTGATG 
GAGGCATCTC AGGAGGGACA CCTGGAATTG GTTAAATATT TGCTGGCTTC TGGCGCTAAT 
GTGCATGCTA CAACAGCAAC AGGAGACACA GCCTTAACCT ATGCTTGTGA AAATGGACAT 
ACGGATGTTG CAGATGTTTT ACTTCAAGCA GGGGCTGATT TAGACAAGCA GGAGGACATG 
AAGACTATTT TGGAGGGCAT AGATCCGGCC AAGCATCAGG TGAGGGTGGC CTTTGATGCT 
TGTAAGCTAC TACGTAAAGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKHD1 ( NM_024668.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_078944.2 Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 3, mRNA.

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CloneID	OHu04164
Clone ID Related Accession (Same CDS sequence)	NM_024668.3 , NM_024668.4
Accession Version	NM_024668.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	ankyrin repeat and KH domain-containing protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA509209.1 and BC004457.1. On May 31, 2019 this sequence version replaced NM_024668.3. Transcript Variant: This variant (3) lacks several exons in the central and 3' coding regions, and uses an alternate 3' terminal exon, compared to variant 1. The resulting isoform (3) is significantly shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC004457.1, SRR1163658.336298.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG GAATTGGCAA ATTGTCAACT 
GCTGATGGTA AAGCTTTTGC AGATCCTGAG GTACTCCGGA GACTGACATC CTCAGTTAGT 
TGTGCACTGG ATGAAGCTGC TGCTGCACTG ACACGGATGA AAGCAGAAAA CAGCCACAAT 
GCAGGACAAG TGGACACTCG CAGTCTAGCA GAAGCTTGTT CAGATGGGGA TGTTAATGCT 
GTTCGTAAAT TGCTAGATGA AGGCAGAAGT GTAAATGAAC ATACAGAAGA AGGAGAAAGC 
CTGCTGTGTT TGGCTTGTTC AGCAGGGTAT TATGAATTAG CACAAGTATT GCTTGCTATG 
CATGCTAATG TTGAAGATCG AGGGAATAAA GGAGACATAA CTCCCCTGAT GGCAGCTTCC 
AGTGGAGGTT ACTTAGATAT TGTGAAATTA TTACTTCTTC ATGATGCTGA TGTCAACTCC 
CAGTCTGCAA CAGGAAACAC TGCGCTAACT TATGCATGTG CTGGAGGATT TGTTGACATT 
GTTAAAGTGC TCCTTAATGA AGGTGCAAAT ATAGAAGATC ATAATGAAAA TGGACATACT 
CCCTTAATGG AAGCAGCCAG TGCAGGTCAT GTGGAAGTTG CAAGAGTTCT TTTAGATCAT 
GGTGCAGGCA TCAACACTCA TTCTAATGAA TTCAAAGAAA GTGCTCTAAC ACTTGCTTGC 
TACAAAGGCC ATTTGGATAT GGTTCGCTTT CTACTTGAAG CTGGTGCAGA TCAAGAGCAC 
AAAACAGATG AGATGCACAC TGCCTTAATG GAGGCCTGCA TGGATGGACA TGTAGAGGTG 
GCACGTTTGC TTTTGGATAG TGGTGCTCAA GTGAACATGC CTGCAGATTC ATTTGAATCT 
CCATTGACGC TAGCTGCCTG TGGAGGACAT GTTGAATTGG CAGCTCTACT TATTGAAAGG 
GGAGCAAATC TTGAAGAAGT TAATGATGAA GGATACACTC CCTTGATGGA AGCTGCCCGG 
GAAGGACATG AAGAAATGGT GGCACTACTC TTAGCACAAG GAGCAAATAT AAATGCCCAG 
ACAGAAGAAA CTCAAGAAAC TGCTCTTACT TTGGCTTGCT GTGGAGGATT TTCTGAAGTT 
GCAGACTTTC TTATTAAGGC AGGGGCTGAT ATAGAACTTG GCTGCTCCAC ACCTCTGATG 
GAGGCATCTC AGGAGGGACA CCTGGAATTG GTTAAATATT TGCTGGCTTC TGGCGCTAAT 
GTGCATGCTA CAACAGCAAC AGGAGACACA GCCTTAACCT ATGCTTGTGA AAATGGACAT 
ACGGATGTTG CAGATGTTTT ACTTCAAGCA GGGGCTGATT TAGACAAGCA GGAGGACATG 
AAGACTATTT TGGAGGGCAT AGATCCGGCC AAGCATCAGG TGAGGGTGGC CTTTGATGCT 
TGTAAGCTAC TACGTAAAGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078944.2
CDS	136..2019
Translation	MLTDSGGGGTSFEEDLDSVAPRSAPAGASEPPPPGGVGLGIRTVRLFGEAGPASGVGSSGGGGSGSGTGGGDAALDFKLAAAVLRTGGGGGASGSDEDEVSEVESFILDQEDLDNPVLKTTSEIFLSSTAEGADLRTVDPETQARLEALLEAAGIGKLSTADGKAFADPEVLRRLTSSVSCALDEAAAALTRMKAENSHNAGQVDTRSLAEACSDGDVNAVRKLLDEGRSVNEHTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGNKGDITPLMAASSGGYLDIVKLLLLHDADVNSQSATGNTALTYACAGGFVDIVKVLLNEGANIEDHNENGHTPLMEAASAGHVEVARVLLDHGAGINTHSNEFKESALTLACYKGHLDMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFSEVADFLIKAGADIELGCSTPLMEASQEGHLELVKYLLASGANVHATTATGDTALTYACENGHTDVADVLLQAGADLDKQEDMKTILEGIDPAKHQVRVAFDACKLLRKE

Target ORF information:

RefSeq Version	NM_024668.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024668.4

ORF Insert Sequence:

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG GAATTGGCAA ATTGTCAACT 
GCTGATGGTA AAGCTTTTGC AGATCCTGAG GTACTCCGGA GACTGACATC CTCAGTTAGT 
TGTGCACTGG ATGAAGCTGC TGCTGCACTG ACACGGATGA AAGCAGAAAA CAGCCACAAT 
GCAGGACAAG TGGACACTCG CAGTCTAGCA GAAGCTTGTT CAGATGGGGA TGTTAATGCT 
GTTCGTAAAT TGCTAGATGA AGGCAGAAGT GTAAATGAAC ATACAGAAGA AGGAGAAAGC 
CTGCTGTGTT TGGCTTGTTC AGCAGGGTAT TATGAATTAG CACAAGTATT GCTTGCTATG 
CATGCTAATG TTGAAGATCG AGGGAATAAA GGAGACATAA CTCCCCTGAT GGCAGCTTCC 
AGTGGAGGTT ACTTAGATAT TGTGAAATTA TTACTTCTTC ATGATGCTGA TGTCAACTCC 
CAGTCTGCAA CAGGAAACAC TGCGCTAACT TATGCATGTG CTGGAGGATT TGTTGACATT 
GTTAAAGTGC TCCTTAATGA AGGTGCAAAT ATAGAAGATC ATAATGAAAA TGGACATACT 
CCCTTAATGG AAGCAGCCAG TGCAGGTCAT GTGGAAGTTG CAAGAGTTCT TTTAGATCAT 
GGTGCAGGCA TCAACACTCA TTCTAATGAA TTCAAAGAAA GTGCTCTAAC ACTTGCTTGC 
TACAAAGGCC ATTTGGATAT GGTTCGCTTT CTACTTGAAG CTGGTGCAGA TCAAGAGCAC 
AAAACAGATG AGATGCACAC TGCCTTAATG GAGGCCTGCA TGGATGGACA TGTAGAGGTG 
GCACGTTTGC TTTTGGATAG TGGTGCTCAA GTGAACATGC CTGCAGATTC ATTTGAATCT 
CCATTGACGC TAGCTGCCTG TGGAGGACAT GTTGAATTGG CAGCTCTACT TATTGAAAGG 
GGAGCAAATC TTGAAGAAGT TAATGATGAA GGATACACTC CCTTGATGGA AGCTGCCCGG 
GAAGGACATG AAGAAATGGT GGCACTACTC TTAGCACAAG GAGCAAATAT AAATGCCCAG 
ACAGAAGAAA CTCAAGAAAC TGCTCTTACT TTGGCTTGCT GTGGAGGATT TTCTGAAGTT 
GCAGACTTTC TTATTAAGGC AGGGGCTGAT ATAGAACTTG GCTGCTCCAC ACCTCTGATG 
GAGGCATCTC AGGAGGGACA CCTGGAATTG GTTAAATATT TGCTGGCTTC TGGCGCTAAT 
GTGCATGCTA CAACAGCAAC AGGAGACACA GCCTTAACCT ATGCTTGTGA AAATGGACAT 
ACGGATGTTG CAGATGTTTT ACTTCAAGCA GGGGCTGATT TAGACAAGCA GGAGGACATG 
AAGACTATTT TGGAGGGCAT AGATCCGGCC AAGCATCAGG TGAGGGTGGC CTTTGATGCT 
TGTAAGCTAC TACGTAAAGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKHD1 ( NM_001197030.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001183959.1 Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu05657
Clone ID Related Accession (Same CDS sequence)	NM_001197030.1
Accession Version	NM_001197030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and KH domain-containing protein 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA509209.1, BC117678.2 and BC004457.1. Transcript Variant: This variant (4) lacks several exons in the central and 3' coding regions, and uses an alternate 3' terminal exon, compared to variant 1. The resulting isoform (4) is significantly shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC117678.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG GAATTGGCAA ATTGTCAACT 
GCTGATGGTA AAGCTTTTGC AGATCCTGAG GTACTCCGGA GACTGACATC CTCAGTTAGT 
TGTGCACTGG ATGAAGCTGC TGCTGCACTG ACACGGATGA AAGCAGAAAA CAGCCACAAT 
GCAGGACAAG TGGACACTCG CAGTCTAGCA GAAGCTTGTT CAGATGGGGA TGTTAATGCT 
GTTCGTAAAT TGCTAGATGA AGGCAGAAGT GTAAATGAAC ATACAGAAGA AGGAGAAAGC 
CTGCTGTGTT TGGCTTGTTC AGCAGGGTAT TATGAATTAG CACAAGTATT GCTTGCTATG 
CATGCTAATG TTGAAGATCG AGGGAATAAA GGAGACATAA CTCCCCTGAT GGCAGCTTCC 
AGTGGAGGTT ACTTAGATAT TGTGAAATTA TTACTTCTTC ATGATGCTGA TGTCAACTCC 
CAGTCTGCAA CAGGAAACAC TGCGCTAACT TATGCATGTG CTGGAGGATT TGTTGACATT 
GTTAAAGTGC TCCTTAATGA AGGTGCAAAT ATAGAAGATC ATAATGAAAA TGGACATACT 
CCCTTAATGG AAGCAGCCAG TGCAGGTCAT GTGGAAGTTG CAAGAGTTCT TTTAGATCAT 
GGTGCAGGCA TCAACACTCA TTCTAATGAA TTCAAAGAAA GTGCTCTAAC ACTTGCTTGC 
TACAAAGGCC ATTTGGATAT GGTTCGCTTT CTACTTGAAG CTGGTGCAGA TCAAGAGCAC 
AAAACAGATG AGATGCACAC TGCCTTAATG GAGGCCTGCA TGGATGGACA TGTAGAGGTG 
GCACGTTTGC TTTTGGATAG TGGTGCTCAA GTGAACATGC CTGCAGATTC ATTTGAATCT 
CCATTGACGC TAGCTGCCTG TGGAGGACAT GTTGAATTGG CAGCTCTACT TATTGAAAGG 
GGAGCAAATC TTGAAGAAGT TAATGATGAA GGATACACTC CCTTGATGGA AGCTGCCCGG 
GAAGGACATG AAGAAATGGT GGCACTACTC TTAGCACAAG GAGCAAATAT AAATGCCCAG 
ACAGAAGAAA CTCAAGAAAC TGCTCTTACT TTGGCTTGCT GTGGAGGATT TTCTGAAGTT 
GCAGACTTTC TTATTAAGGC AGGGGCTGAT ATAGAACTTG GCTGCTCCAC ACCTCTGATG 
GAGGCATCTC AGGAGGGACA CCTGGAATTG GTTAAATATT TGCTGGCTTC TGGACAAGCA 
GGAGGACATG AAGACTATTT TGGAGGGCAT AGATCCGGCC AAGCATCAGG TGAGGGTGGC 
CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001183959.1
CDS	155..1900
Translation	MLTDSGGGGTSFEEDLDSVAPRSAPAGASEPPPPGGVGLGIRTVRLFGEAGPASGVGSSGGGGSGSGTGGGDAALDFKLAAAVLRTGGGGGASGSDEDEVSEVESFILDQEDLDNPVLKTTSEIFLSSTAEGADLRTVDPETQARLEALLEAAGIGKLSTADGKAFADPEVLRRLTSSVSCALDEAAAALTRMKAENSHNAGQVDTRSLAEACSDGDVNAVRKLLDEGRSVNEHTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGNKGDITPLMAASSGGYLDIVKLLLLHDADVNSQSATGNTALTYACAGGFVDIVKVLLNEGANIEDHNENGHTPLMEAASAGHVEVARVLLDHGAGINTHSNEFKESALTLACYKGHLDMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFSEVADFLIKAGADIELGCSTPLMEASQEGHLELVKYLLASGQAGGHEDYFGGHRSGQASGEGGL

Target ORF information:

RefSeq Version	NM_001197030.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001197030.1

ORF Insert Sequence:

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG GAATTGGCAA ATTGTCAACT 
GCTGATGGTA AAGCTTTTGC AGATCCTGAG GTACTCCGGA GACTGACATC CTCAGTTAGT 
TGTGCACTGG ATGAAGCTGC TGCTGCACTG ACACGGATGA AAGCAGAAAA CAGCCACAAT 
GCAGGACAAG TGGACACTCG CAGTCTAGCA GAAGCTTGTT CAGATGGGGA TGTTAATGCT 
GTTCGTAAAT TGCTAGATGA AGGCAGAAGT GTAAATGAAC ATACAGAAGA AGGAGAAAGC 
CTGCTGTGTT TGGCTTGTTC AGCAGGGTAT TATGAATTAG CACAAGTATT GCTTGCTATG 
CATGCTAATG TTGAAGATCG AGGGAATAAA GGAGACATAA CTCCCCTGAT GGCAGCTTCC 
AGTGGAGGTT ACTTAGATAT TGTGAAATTA TTACTTCTTC ATGATGCTGA TGTCAACTCC 
CAGTCTGCAA CAGGAAACAC TGCGCTAACT TATGCATGTG CTGGAGGATT TGTTGACATT 
GTTAAAGTGC TCCTTAATGA AGGTGCAAAT ATAGAAGATC ATAATGAAAA TGGACATACT 
CCCTTAATGG AAGCAGCCAG TGCAGGTCAT GTGGAAGTTG CAAGAGTTCT TTTAGATCAT 
GGTGCAGGCA TCAACACTCA TTCTAATGAA TTCAAAGAAA GTGCTCTAAC ACTTGCTTGC 
TACAAAGGCC ATTTGGATAT GGTTCGCTTT CTACTTGAAG CTGGTGCAGA TCAAGAGCAC 
AAAACAGATG AGATGCACAC TGCCTTAATG GAGGCCTGCA TGGATGGACA TGTAGAGGTG 
GCACGTTTGC TTTTGGATAG TGGTGCTCAA GTGAACATGC CTGCAGATTC ATTTGAATCT 
CCATTGACGC TAGCTGCCTG TGGAGGACAT GTTGAATTGG CAGCTCTACT TATTGAAAGG 
GGAGCAAATC TTGAAGAAGT TAATGATGAA GGATACACTC CCTTGATGGA AGCTGCCCGG 
GAAGGACATG AAGAAATGGT GGCACTACTC TTAGCACAAG GAGCAAATAT AAATGCCCAG 
ACAGAAGAAA CTCAAGAAAC TGCTCTTACT TTGGCTTGCT GTGGAGGATT TTCTGAAGTT 
GCAGACTTTC TTATTAAGGC AGGGGCTGAT ATAGAACTTG GCTGCTCCAC ACCTCTGATG 
GAGGCATCTC AGGAGGGACA CCTGGAATTG GTTAAATATT TGCTGGCTTC TGGACAAGCA 
GGAGGACATG AAGACTATTT TGGAGGGCAT AGATCCGGCC AAGCATCAGG TGAGGGTGGC 
CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKHD1 ( NM_017747.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060217.1 Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 1, mRNA.

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CloneID	OHu02533
Clone ID Related Accession (Same CDS sequence)	NM_017747.2 , NM_017747.3
Accession Version	NM_017747.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	ankyrin repeat and KH domain-containing protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA509209.1, AF521882.1, AK000904.1 and AI675547.1. On Oct 1, 2010 this sequence version replaced NM_017747.1. Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF521882.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG GAATTGGCAA ATTGTCAACT 
GCTGATGGTA AAGCTTTTGC AGATCCTGAG GTACTCCGGA GACTGACATC CTCAGTTAGT 
TGTGCACTGG ATGAAGCTGC TGCTGCACTG ACACGGATGA AAGCAGAAAA CAGCCACAAT 
GCAGGACAAG TGGACACTCG CAGTCTAGCA GAAGCTTGTT CAGATGGGGA TGTTAATGCT 
GTTCGTAAAT TGCTAGATGA AGGCAGAAGT GTAAATGAAC ATACAGAAGA AGGAGAAAGC 
CTGCTGTGTT TGGCTTGTTC AGCAGGGTAT TATGAATTAG CACAAGTATT GCTTGCTATG 
CATGCTAATG TTGAAGATCG AGGGAATAAA GGAGACATAA CTCCCCTGAT GGCAGCTTCC 
AGTGGAGGTT ACTTAGATAT TGTGAAATTA TTACTTCTTC ATGATGCTGA TGTCAACTCC 
CAGTCTGCAA CAGGAAACAC TGCGCTAACT TATGCATGTG CTGGAGGATT TGTTGACATT 
GTTAAAGTGC TCCTTAATGA AGGTGCAAAT ATAGAAGATC ATAATGAAAA TGGACATACT 
CCCTTAATGG AAGCAGCCAG TGCAGGTCAT GTGGAAGTTG CAAGAGTTCT TTTAGATCAT 
GGTGCAGGCA TCAACACTCA TTCTAATGAA TTCAAAGAAA GTGCTCTAAC ACTTGCTTGC 
TACAAAGGCC ATTTGGATAT GGTTCGCTTT CTACTTGAAG CTGGTGCAGA TCAAGAGCAC 
AAAACAGATG AGATGCACAC TGCCTTAATG GAGGCCTGCA TGGATGGACA TGTAGAGGTG 
GCACGTTTGC TTTTGGATAG TGGTGCTCAA GTGAACATGC CTGCAGATTC ATTTGAATCT 
CCATTGACGC TAGCTGCCTG TGGAGGACAT GTTGAATTGG CAGCTCTACT TATTGAAAGG 
GGAGCAAATC TTGAAGAAGT TAATGATGAA GGATACACTC CCTTGATGGA AGCTGCCCGG 
GAAGGACATG AAGAAATGGT GGCACTACTC TTAGCACAAG GAGCAAATAT AAATGCCCAG 
ACAGAAGAAA CTCAAGAAAC TGCTCTTACT TTGGCTTGCT GTGGAGGATT TTCTGAAGTT 
GCAGACTTTC TTATTAAGGC AGGGGCTGAT ATAGAACTTG GCTGCTCCAC ACCTCTGATG 
GAGGCATCTC AGGAGGGACA CCTGGAATTG GTTAAATATT TGCTGGCTTC TGGCGCTAAT 
GTGCATGCTA CAACAGCAAC AGGAGACACA GCCTTAACCT ATGCTTGTGA AAATGGACAT 
ACGGATGTTG CAGATGTTTT ACTTCAAGCA GGGGCTGATT TAGAACATGA ATCTGAAGGT 
GGAAGAACAC CTTTGATGAA AGCTGCAAGA GCTGGTCATT TGTGCACTGT GCAGTTTCTT 
ATTAGCAAAG GTGCCAATGT TAACAGGGCT ACAGCCAATA ATGATCATAC AGTAGTGTCG 
CTGGCATGTG CAGGAGGCCA CCTGGCAGTT GTTGAGCTTC TCTTGGCTCA TGGGGCTGAC 
CCTACTCATC GACTCAAGGA TGGTTCAACA ATGCTCATTG AAGCTGCAAA GGGTGGCCAT 
ACTAATGTAG TTTCTTATCT GTTGGATTAT CCAAATAATG TTCTGTCAGT TCCCACCACA 
GATGTGTCTC AGCTCCCTCC ACCTTCTCAA GATCAGTCTC AGGTGCCACG TGTGCCAACG 
CATACACTTG CCATGGTTGT ACCTCCCCAG GAACCTGACA GAACTTCACA GGAGAACTCT 
CCTGCCCTTT TAGGAGTGCA AAAAGGTACA TCCAAGCAGA AGTCCAGTTC CCTCCAGGTA 
GCAGATCAGG ACCTACTGCC ATCTTTTCAC CCATACCAGC CTTTGGAGTG CATAGTAGAG 
GAGACTGAAG GCAAGCTGAA TGAACTGGGA CAAAGAATTA GTGCTATTGA AAAAGCACAG 
CTTAAGTCAC TGGAGTTAAT TCAAGGTGAA CCTCTGAACA AAGATAAGAT AGAAGAACTT 
AAAAAGAACA GAGAAGAGCA AGTCCAGAAG AAGAAGAAAA TATTGAAAGA ACTGCAGAAA 
GTGGAAAGGC AGTTGCAGAT GAAAACACAG CAGCAATTTA CCAAAGAATA CTTGGAAACC 
AAAGGTCAGA AAGACACAGT GTCTCTACAC CAACAGTGCT CTCATAGAGG AGTCTTCCCA 
GAAGGGGAAG GAGATGGTAG TCTCCCAGAG GATCACTTTT CAGAGTTACC TCAGGTTGAC 
ACAATCTTAT TTAAAGATAA TGATGTTGAT GATGAGCAAC AGTCTCCACC ATCGGCAGAA 
CAGATTGATT TTGTCCCAGT CCAGCCTTTA TCATCTCCAC AGTGTAACTT TTCCAGTGAC 
TTAGGTTCTA ATGGGACAAA TTCTCTTGAA CTTCAGAAAG TATCAGGTAA TCAGCAGATT 
GTAGGACAGC CTCAGATTGC TATTACTGGA CATGATCAGG GGCTGTTAGT TCAAGAACCA 
GATGGACTAA TGGTTGCAAC TCCAGCTCAG ACGCTTACCG ACACTCTTGA TGACCTGATA 
GCAGCTGTGA GTACCAGAGT GCCCACTGGT TCCAACAGTT CTTCTCAGAC CACAGAGTGT 
CTTACACCTG AATCCTGTTC GCAGACTACA AGCAATGTGG CTTCCCAATC GATGCCTCCT 
GTGTATCCTT CAGTTGACAT TGATGCACAT ACTGAGAGCA ATCATGACAC AGCATTAACA 
CTAGCTTGTG CAGGTGGTCA TGAAGAACTT GTATCTGTGC TCATTGCACG GGATGCCAAA 
ATTGAACACA GAGACAAAAA AGGTTTCACA CCACTAATCC TGGCAGCAAC AGCAGGGCAT 
GTTGGAGTTG TTGAAATCCT TTTGGATAAA GGTGGAGATA TAGAAGCACA GTCTGAACGA 
ACTAAGGATA CTCCGCTTTC ATTGGCATGT TCTGGTGGAC GTCAGGAGGT GGTAGACTTG 
CTGCTGGCTC GAGGTGCAAA TAAAGAACAT AGGAACGTAT CTGATTATAC ACCACTGAGT 
CTAGCTGCGT CTGGAGGATA TGTTAATATC ATTAAGATTC TGCTTAATGC TGGGGCAGAA 
ATTAATTCAA GGACTGGGAG TAAACTAGGT ATTTCTCCCC TGATGTTGGC TGCAATGAAT 
GGACATGTTC CTGCAGTAAA ATTGCTGCTC GATATGGGTT CAGACATTAA TGCCCAAATA 
GAGACCAATC GGAACACGGC TCTCACCCTG GCCTGTTTCC AGGGCCGAGC AGAAGTAGTG 
AGTTTGCTTC TGGACCGAAA AGCCAATGTT GAACATAGGG CAAAGACGGG TCTTACCCCC 
TTGATGGAAG CAGCTTCTGG AGGGTATGCA GAGGTTGGAA GAGTTCTTCT TGATAAAGGA 
GCAGATGTTA ATGCTCCCCC TGTGCCTTCC TCAAGAGATA CTGCTTTAAC AATAGCAGCA 
GACAAAGGTC ACTACAAATT TTGTGAACTC CTGATTCATA GGGGAGCCCA CATTGATGTT 
CGTAACAAAA AGGGAAATAC GCCACTTTGG CTGGCATCCA ATGGAGGTCA TTTTGATGTT 
GTGCAGTTGC TAGTGCAAGC AGGTGCTGAT GTGGATGCAG CAGATAACCG GAAAATCACA 
CCTCTTATGT CAGCATTTCG CAAGGGTCAT GTAAAAGTTG TTCAATATTT GGTAAAGGAA 
GTAAATCAGT TCCCTTCTGA TATAGAATGC ATGAGATACA TAGCAACAAT TACAGATAAG 
GAACTGTTGA AAAAATGTCA TCAATGTGTC GAAACCATTG TGAAGGCTAA AGACCAGCAA 
GCTGCAGAAG CAAATAAGAA TGCGAGTATT CTTTTAAAGG AACTTGATCT GGAAAAGTCA 
AGAGAAGAGA GCAGAAAGCA GGCTCTTGCT GCTAAAAGAG AAAAAAGAAA AGAAAAGAGA 
AAAAAGAAAA AAGAGGAACA GAAAAGGAAA CAGGAAGAAG ATGAAGAAAA CAAACCTAAG 
GAGAATTCGG AACTACCAGA GGATGAAGAT GAAGAGGAGA ATGATGAAGA TGTGGAGCAA 
GAAGTTCCCA TAGAACCTCC TAGTGCAACC ACCACCACTA CGATTGGAAT CTCTGCAACA 
TCTGCAACAT TCACAAATGT GTTTGGGAAA AAAAGGGCCA ATGTGGTGAC AACTCCCAGC 
ACCAATCGGA AAAATAAGAA GAACAAAACA AAAGAAACCC CTCCTACAGC ACATTTAATT 
TTACCAGAAC AACATATGTC TTTAGCCCAA CAAAAGGCAG ATAAAAATAA AATAAATGGA 
GAACCTAGAG GTGGTGGTGC AGGTGGGAAT AGTGATTCAG ATAACTTGGA CAGCACAGAC 
TGCAACAGTG AGAGTAGCAG TGGTGGTAAA AGCCAAGAGT TAAATTTTGT GATGGATGTG 
AATTCCTCTA AATACCCCTC ACTGCTCCTT CATTCCCAAG AAGAAAAGAC AAGTACTGCT 
ACTTCCAAAA CTCAGACACG ACTTGAAGGT GAAGTGACTC CTAATTCCTT GTCAACCAGC 
TACAAGACAG TGTCATTGCC ATTAAGCTCT CCAAACATAA AGCTGAATCT CACTAGCCCT 
AAAAGGGGTC AGAAAAGAGA AGAAGGGTGG AAAGAAGTTG TACGAAGGTC AAAGAAATTG 
TCTGTTCCAG CCTCAGTGGT GTCGAGGATA ATGGGAAGAG GAGGATGCAA CATCACTGCA 
ATACAGGATG TTACTGGTGC CCATATTGAT GTGGATAAAC AAAAAGATAA GAATGGCGAG 
AGAATGATCA CAATAAGGGG TGGCACAGAA TCAACAAGAT ATGCAGTTCA ACTAATCAAT 
GCACTCATTC AAGATCCTGC TAAGGAACTG GAAGACTTGA TTCCTAAAAA TCATATCAGA 
ACACCTGCCA GCACCAAATC AATTCATGCT AACTTCTCAT CTGGAGTAGG TACCACAGCA 
GCTTCCAGTA AAAATGCATT TCCTTTGGGT GCTCCAACTC TTGTAACTTC ACAGGCAACA 
ACGTTATCTA CGTTCCAGCC CGCTAATAAA CTTAATAAGA ATGTTCCAAC AAATGTACGT 
TCTTCTTTCC CAGTTTCTCT ACCCTTAGCT TATCCTCACC CTCATTTTGC CCTGCTGGCT 
GCTCAAACTA TGCAACAGAT TCGGCATCCT CGCTTACCCA TGGCCCAGTT TGGAGGAACC 
TTCTCACCTT CTCCTAACAC ATGGGGACCA TTCCCAGTGA GACCTGTGAA TCCTGGCAAC 
ACAAATAGCT CTCCAAAGCA TAATAACACA AGCCGTCTAC CTAACCAGAA CGGGACTGTT 
TTACCCTCAG AGTCTGCTGG ACTAGCTACT GCCAGTTGTC CTATCACTGT CTCTTCTGTA 
GTTGCTGCCA GTCAGCAACT GTGTGTCACT AATACCCGGA CTCCTTCATC AGTCAGAAAG 
CAGTTGTTTG CCTGTGTGCC TAAGACAAGT CCTCCAGCAA CAGTGATTTC TTCTGTGACA 
AGCACTTGTA GTTCCCTGCC TTCTGTCTCC TCTGCACCTA TCACTAGCGG GCAAGCTCCC 
ACCACATTTC TACCTGCAAG TACTTCTCAA GCACAGCTTT CTTCACAAAA GATGGAGTCT 
TTCTCTGCTG TGCCACCCAC CAAAGAGAAA GTGTCCACAC AGGACCAGCC CATGGCAAAC 
CTATGTACCC CATCTTCAAC TGCAAACAGT TGCAGTAGCT CTGCCAGCAA CACCCCGGGA 
GCTCCAGAAA CTCACCCATC CAGTAGTCCC ACTCCTACTT CCAGTAACAC ACAAGAGGAG 
GCACAGCCAT CCAGTGTGTC TGATTTAAGT CCTATGTCAA TGCCTTTTGC ATCTAACTCA 
GAACCTGCTC CATTGACTTT GACATCACCC AGAATGGTTG CTGCTGATAA TCAGGACACC 
AGTAATTTAC CTCAGTTAGC TGTACCAGCA CCTCGAGTTT CTCATCGAAT GCAGCCCAGA 
GGTTCTTTTT ACTCCATGGT ACCAAATGCA ACTATTCACC AGGATCCCCA GTCTATTTTT 
GTTACGAATC CAGTTACTTT AACACCACCT CAAGGCCCAC CAGCTGCAGT GCAGCTTTCT 
TCAGCTGTGA ACATTATGAA TGGTTCTCAG ATGCACATAA ACCCAGCAAA TAAGTCTTTG 
CCACCTACAT TTGGCCCAGC CACACTTTTC AATCACTTCA GCAGTCTTTT TGATAGTAGT 
CAGGTGCCAG CTAACCAGGG CTGGGGAGAT GGTCCACTGT CCTCACGAGT TGCTACAGAT 
GCCTCTTTCA CTGTTCAGTC AGCGTTCCTG GGTAACTCAG TGCTTGGACA CTTGGAAAAC 
ATGCACCCTG ATAACTCAAA GGCACCTGGC TTCAGACCAC CTTCCCAGCG AGTTTCTACT 
AGTCCAGTTG GGTTACCATC CATTGACCCA TCAGGCAGCT CCCCATCTTC CTCTTCTGCT 
CCTCTGGCAA GTTTTTCCGG CATACCAGGA ACAAGGGTTT TCCTGCAAGG GCCAGCTCCT 
GTTGGGACTC CTAGTTTCAA CAGACAACAT TTTTCTCCCC ATCCTTGGAC AAGCGCCTCA 
AACTCATCCA CTTCTGCCCC ACCAACGTTG GGCCAACCAA AAGGAGTCAG TGCCAGTCAA 
GATCGAAAGA TACCTCCCCC AATTGGAACA GAGAGACTGG CCCGAATTCG GCAAGGAGGG 
TCTGTTGCAC AAGCCCCGGC GGGGACCAGT TTTGTCGCTC CCGTTGGACA CAGTGGAATC 
TGGTCATTTG GTGTCAATGC TGTGTCAGAA GGCTTATCAG GTTGGTCGCA ATCTGTGATG 
GGGAACCATC CAATGCATCA ACAATTATCA GACCCAAGCA CATTCTCCCA ACATCAGCCA 
ATGGAGAGAG ATGATTCTGG AATGGTAGCC CCCTCTAACA TTTTTCATCA GCCTATGGCA 
AGTGGTTTTG TGGATTTTTC TAAAGGTCTG CCAATTTCCA TGTATGGAGG CACCATAATA 
CCCTCTCATC CTCAGCTTGC TGATGTTCCA GGAGGCCCTC TGTTTAATGG ACTTCACAAT 
CCAGATCCTG CTTGGAACCC TATGATAAAA GTTATCCAAA ATTCAACTGA ATGCACTGAT 
GCCCAGCAGA TTTGGCCTGG CACGTGGGCA CCTCATATTG GAAACATGCA TCTCAAATAT 
GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060217.1
CDS	155..7783
Translation	MLTDSGGGGTSFEEDLDSVAPRSAPAGASEPPPPGGVGLGIRTVRLFGEAGPASGVGSSGGGGSGSGTGGGDAALDFKLAAAVLRTGGGGGASGSDEDEVSEVESFILDQEDLDNPVLKTTSEIFLSSTAEGADLRTVDPETQARLEALLEAAGIGKLSTADGKAFADPEVLRRLTSSVSCALDEAAAALTRMKAENSHNAGQVDTRSLAEACSDGDVNAVRKLLDEGRSVNEHTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGNKGDITPLMAASSGGYLDIVKLLLLHDADVNSQSATGNTALTYACAGGFVDIVKVLLNEGANIEDHNENGHTPLMEAASAGHVEVARVLLDHGAGINTHSNEFKESALTLACYKGHLDMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFSEVADFLIKAGADIELGCSTPLMEASQEGHLELVKYLLASGANVHATTATGDTALTYACENGHTDVADVLLQAGADLEHESEGGRTPLMKAARAGHLCTVQFLISKGANVNRATANNDHTVVSLACAGGHLAVVELLLAHGADPTHRLKDGSTMLIEAAKGGHTNVVSYLLDYPNNVLSVPTTDVSQLPPPSQDQSQVPRVPTHTLAMVVPPQEPDRTSQENSPALLGVQKGTSKQKSSSLQVADQDLLPSFHPYQPLECIVEETEGKLNELGQRISAIEKAQLKSLELIQGEPLNKDKIEELKKNREEQVQKKKKILKELQKVERQLQMKTQQQFTKEYLETKGQKDTVSLHQQCSHRGVFPEGEGDGSLPEDHFSELPQVDTILFKDNDVDDEQQSPPSAEQIDFVPVQPLSSPQCNFSSDLGSNGTNSLELQKVSGNQQIVGQPQIAITGHDQGLLVQEPDGLMVATPAQTLTDTLDDLIAAVSTRVPTGSNSSSQTTECLTPESCSQTTSNVASQSMPPVYPSVDIDAHTESNHDTALTLACAGGHEELVSVLIARDAKIEHRDKKGFTPLILAATAGHVGVVEILLDKGGDIEAQSERTKDTPLSLACSGGRQEVVDLLLARGANKEHRNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHVPAVKLLLDMGSDINAQIETNRNTALTLACFQGRAEVVSLLLDRKANVEHRAKTGLTPLMEAASGGYAEVGRVLLDKGADVNAPPVPSSRDTALTIAADKGHYKFCELLIHRGAHIDVRNKKGNTPLWLASNGGHFDVVQLLVQAGADVDAADNRKITPLMSAFRKGHVKVVQYLVKEVNQFPSDIECMRYIATITDKELLKKCHQCVETIVKAKDQQAAEANKNASILLKELDLEKSREESRKQALAAKREKRKEKRKKKKEEQKRKQEEDEENKPKENSELPEDEDEEENDEDVEQEVPIEPPSATTTTTIGISATSATFTNVFGKKRANVVTTPSTNRKNKKNKTKETPPTAHLILPEQHMSLAQQKADKNKINGEPRGGGAGGNSDSDNLDSTDCNSESSSGGKSQELNFVMDVNSSKYPSLLLHSQEEKTSTATSKTQTRLEGEVTPNSLSTSYKTVSLPLSSPNIKLNLTSPKRGQKREEGWKEVVRRSKKLSVPASVVSRIMGRGGCNITAIQDVTGAHIDVDKQKDKNGERMITIRGGTESTRYAVQLINALIQDPAKELEDLIPKNHIRTPASTKSIHANFSSGVGTTAASSKNAFPLGAPTLVTSQATTLSTFQPANKLNKNVPTNVRSSFPVSLPLAYPHPHFALLAAQTMQQIRHPRLPMAQFGGTFSPSPNTWGPFPVRPVNPGNTNSSPKHNNTSRLPNQNGTVLPSESAGLATASCPITVSSVVAASQQLCVTNTRTPSSVRKQLFACVPKTSPPATVISSVTSTCSSLPSVSSAPITSGQAPTTFLPASTSQAQLSSQKMESFSAVPPTKEKVSTQDQPMANLCTPSSTANSCSSSASNTPGAPETHPSSSPTPTSSNTQEEAQPSSVSDLSPMSMPFASNSEPAPLTLTSPRMVAADNQDTSNLPQLAVPAPRVSHRMQPRGSFYSMVPNATIHQDPQSIFVTNPVTLTPPQGPPAAVQLSSAVNIMNGSQMHINPANKSLPPTFGPATLFNHFSSLFDSSQVPANQGWGDGPLSSRVATDASFTVQSAFLGNSVLGHLENMHPDNSKAPGFRPPSQRVSTSPVGLPSIDPSGSSPSSSSAPLASFSGIPGTRVFLQGPAPVGTPSFNRQHFSPHPWTSASNSSTSAPPTLGQPKGVSASQDRKIPPPIGTERLARIRQGGSVAQAPAGTSFVAPVGHSGIWSFGVNAVSEGLSGWSQSVMGNHPMHQQLSDPSTFSQHQPMERDDSGMVAPSNIFHQPMASGFVDFSKGLPISMYGGTIIPSHPQLADVPGGPLFNGLHNPDPAWNPMIKVIQNSTECTDAQQIWPGTWAPHIGNMHLKYVN

Target ORF information:

RefSeq Version	NM_017747.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017747.2

ORF Insert Sequence:

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG GAATTGGCAA ATTGTCAACT 
GCTGATGGTA AAGCTTTTGC AGATCCTGAG GTACTCCGGA GACTGACATC CTCAGTTAGT 
TGTGCACTGG ATGAAGCTGC TGCTGCACTG ACACGGATGA AAGCAGAAAA CAGCCACAAT 
GCAGGACAAG TGGACACTCG CAGTCTAGCA GAAGCTTGTT CAGATGGGGA TGTTAATGCT 
GTTCGTAAAT TGCTAGATGA AGGCAGAAGT GTAAATGAAC ATACAGAAGA AGGAGAAAGC 
CTGCTGTGTT TGGCTTGTTC AGCAGGGTAT TATGAATTAG CACAAGTATT GCTTGCTATG 
CATGCTAATG TTGAAGATCG AGGGAATAAA GGAGACATAA CTCCCCTGAT GGCAGCTTCC 
AGTGGAGGTT ACTTAGATAT TGTGAAATTA TTACTTCTTC ATGATGCTGA TGTCAACTCC 
CAGTCTGCAA CAGGAAACAC TGCGCTAACT TATGCATGTG CTGGAGGATT TGTTGACATT 
GTTAAAGTGC TCCTTAATGA AGGTGCAAAT ATAGAAGATC ATAATGAAAA TGGACATACT 
CCCTTAATGG AAGCAGCCAG TGCAGGTCAT GTGGAAGTTG CAAGAGTTCT TTTAGATCAT 
GGTGCAGGCA TCAACACTCA TTCTAATGAA TTCAAAGAAA GTGCTCTAAC ACTTGCTTGC 
TACAAAGGCC ATTTGGATAT GGTTCGCTTT CTACTTGAAG CTGGTGCAGA TCAAGAGCAC 
AAAACAGATG AGATGCACAC TGCCTTAATG GAGGCCTGCA TGGATGGACA TGTAGAGGTG 
GCACGTTTGC TTTTGGATAG TGGTGCTCAA GTGAACATGC CTGCAGATTC ATTTGAATCT 
CCATTGACGC TAGCTGCCTG TGGAGGACAT GTTGAATTGG CAGCTCTACT TATTGAAAGG 
GGAGCAAATC TTGAAGAAGT TAATGATGAA GGATACACTC CCTTGATGGA AGCTGCCCGG 
GAAGGACATG AAGAAATGGT GGCACTACTC TTAGCACAAG GAGCAAATAT AAATGCCCAG 
ACAGAAGAAA CTCAAGAAAC TGCTCTTACT TTGGCTTGCT GTGGAGGATT TTCTGAAGTT 
GCAGACTTTC TTATTAAGGC AGGGGCTGAT ATAGAACTTG GCTGCTCCAC ACCTCTGATG 
GAGGCATCTC AGGAGGGACA CCTGGAATTG GTTAAATATT TGCTGGCTTC TGGCGCTAAT 
GTGCATGCTA CAACAGCAAC AGGAGACACA GCCTTAACCT ATGCTTGTGA AAATGGACAT 
ACGGATGTTG CAGATGTTTT ACTTCAAGCA GGGGCTGATT TAGAACATGA ATCTGAAGGT 
GGAAGAACAC CTTTGATGAA AGCTGCAAGA GCTGGTCATT TGTGCACTGT GCAGTTTCTT 
ATTAGCAAAG GTGCCAATGT TAACAGGGCT ACAGCCAATA ATGATCATAC AGTAGTGTCG 
CTGGCATGTG CAGGAGGCCA CCTGGCAGTT GTTGAGCTTC TCTTGGCTCA TGGGGCTGAC 
CCTACTCATC GACTCAAGGA TGGTTCAACA ATGCTCATTG AAGCTGCAAA GGGTGGCCAT 
ACTAATGTAG TTTCTTATCT GTTGGATTAT CCAAATAATG TTCTGTCAGT TCCCACCACA 
GATGTGTCTC AGCTCCCTCC ACCTTCTCAA GATCAGTCTC AGGTGCCACG TGTGCCAACG 
CATACACTTG CCATGGTTGT ACCTCCCCAG GAACCTGACA GAACTTCACA GGAGAACTCT 
CCTGCCCTTT TAGGAGTGCA AAAAGGTACA TCCAAGCAGA AGTCCAGTTC CCTCCAGGTA 
GCAGATCAGG ACCTACTGCC ATCTTTTCAC CCATACCAGC CTTTGGAGTG CATAGTAGAG 
GAGACTGAAG GCAAGCTGAA TGAACTGGGA CAAAGAATTA GTGCTATTGA AAAAGCACAG 
CTTAAGTCAC TGGAGTTAAT TCAAGGTGAA CCTCTGAACA AAGATAAGAT AGAAGAACTT 
AAAAAGAACA GAGAAGAGCA AGTCCAGAAG AAGAAGAAAA TATTGAAAGA ACTGCAGAAA 
GTGGAAAGGC AGTTGCAGAT GAAAACACAG CAGCAATTTA CCAAAGAATA CTTGGAAACC 
AAAGGTCAGA AAGACACAGT GTCTCTACAC CAACAGTGCT CTCATAGAGG AGTCTTCCCA 
GAAGGGGAAG GAGATGGTAG TCTCCCAGAG GATCACTTTT CAGAGTTACC TCAGGTTGAC 
ACAATCTTAT TTAAAGATAA TGATGTTGAT GATGAGCAAC AGTCTCCACC ATCGGCAGAA 
CAGATTGATT TTGTCCCAGT CCAGCCTTTA TCATCTCCAC AGTGTAACTT TTCCAGTGAC 
TTAGGTTCTA ATGGGACAAA TTCTCTTGAA CTTCAGAAAG TATCAGGTAA TCAGCAGATT 
GTAGGACAGC CTCAGATTGC TATTACTGGA CATGATCAGG GGCTGTTAGT TCAAGAACCA 
GATGGACTAA TGGTTGCAAC TCCAGCTCAG ACGCTTACCG ACACTCTTGA TGACCTGATA 
GCAGCTGTGA GTACCAGAGT GCCCACTGGT TCCAACAGTT CTTCTCAGAC CACAGAGTGT 
CTTACACCTG AATCCTGTTC GCAGACTACA AGCAATGTGG CTTCCCAATC GATGCCTCCT 
GTGTATCCTT CAGTTGACAT TGATGCACAT ACTGAGAGCA ATCATGACAC AGCATTAACA 
CTAGCTTGTG CAGGTGGTCA TGAAGAACTT GTATCTGTGC TCATTGCACG GGATGCCAAA 
ATTGAACACA GAGACAAAAA AGGTTTCACA CCACTAATCC TGGCAGCAAC AGCAGGGCAT 
GTTGGAGTTG TTGAAATCCT TTTGGATAAA GGTGGAGATA TAGAAGCACA GTCTGAACGA 
ACTAAGGATA CTCCGCTTTC ATTGGCATGT TCTGGTGGAC GTCAGGAGGT GGTAGACTTG 
CTGCTGGCTC GAGGTGCAAA TAAAGAACAT AGGAACGTAT CTGATTATAC ACCACTGAGT 
CTAGCTGCGT CTGGAGGATA TGTTAATATC ATTAAGATTC TGCTTAATGC TGGGGCAGAA 
ATTAATTCAA GGACTGGGAG TAAACTAGGT ATTTCTCCCC TGATGTTGGC TGCAATGAAT 
GGACATGTTC CTGCAGTAAA ATTGCTGCTC GATATGGGTT CAGACATTAA TGCCCAAATA 
GAGACCAATC GGAACACGGC TCTCACCCTG GCCTGTTTCC AGGGCCGAGC AGAAGTAGTG 
AGTTTGCTTC TGGACCGAAA AGCCAATGTT GAACATAGGG CAAAGACGGG TCTTACCCCC 
TTGATGGAAG CAGCTTCTGG AGGGTATGCA GAGGTTGGAA GAGTTCTTCT TGATAAAGGA 
GCAGATGTTA ATGCTCCCCC TGTGCCTTCC TCAAGAGATA CTGCTTTAAC AATAGCAGCA 
GACAAAGGTC ACTACAAATT TTGTGAACTC CTGATTCATA GGGGAGCCCA CATTGATGTT 
CGTAACAAAA AGGGAAATAC GCCACTTTGG CTGGCATCCA ATGGAGGTCA TTTTGATGTT 
GTGCAGTTGC TAGTGCAAGC AGGTGCTGAT GTGGATGCAG CAGATAACCG GAAAATCACA 
CCTCTTATGT CAGCATTTCG CAAGGGTCAT GTAAAAGTTG TTCAATATTT GGTAAAGGAA 
GTAAATCAGT TCCCTTCTGA TATAGAATGC ATGAGATACA TAGCAACAAT TACAGATAAG 
GAACTGTTGA AAAAATGTCA TCAATGTGTC GAAACCATTG TGAAGGCTAA AGACCAGCAA 
GCTGCAGAAG CAAATAAGAA TGCGAGTATT CTTTTAAAGG AACTTGATCT GGAAAAGTCA 
AGAGAAGAGA GCAGAAAGCA GGCTCTTGCT GCTAAAAGAG AAAAAAGAAA AGAAAAGAGA 
AAAAAGAAAA AAGAGGAACA GAAAAGGAAA CAGGAAGAAG ATGAAGAAAA CAAACCTAAG 
GAGAATTCGG AACTACCAGA GGATGAAGAT GAAGAGGAGA ATGATGAAGA TGTGGAGCAA 
GAAGTTCCCA TAGAACCTCC TAGTGCAACC ACCACCACTA CGATTGGAAT CTCTGCAACA 
TCTGCAACAT TCACAAATGT GTTTGGGAAA AAAAGGGCCA ATGTGGTGAC AACTCCCAGC 
ACCAATCGGA AAAATAAGAA GAACAAAACA AAAGAAACCC CTCCTACAGC ACATTTAATT 
TTACCAGAAC AACATATGTC TTTAGCCCAA CAAAAGGCAG ATAAAAATAA AATAAATGGA 
GAACCTAGAG GTGGTGGTGC AGGTGGGAAT AGTGATTCAG ATAACTTGGA CAGCACAGAC 
TGCAACAGTG AGAGTAGCAG TGGTGGTAAA AGCCAAGAGT TAAATTTTGT GATGGATGTG 
AATTCCTCTA AATACCCCTC ACTGCTCCTT CATTCCCAAG AAGAAAAGAC AAGTACTGCT 
ACTTCCAAAA CTCAGACACG ACTTGAAGGT GAAGTGACTC CTAATTCCTT GTCAACCAGC 
TACAAGACAG TGTCATTGCC ATTAAGCTCT CCAAACATAA AGCTGAATCT CACTAGCCCT 
AAAAGGGGTC AGAAAAGAGA AGAAGGGTGG AAAGAAGTTG TACGAAGGTC AAAGAAATTG 
TCTGTTCCAG CCTCAGTGGT GTCGAGGATA ATGGGAAGAG GAGGATGCAA CATCACTGCA 
ATACAGGATG TTACTGGTGC CCATATTGAT GTGGATAAAC AAAAAGATAA GAATGGCGAG 
AGAATGATCA CAATAAGGGG TGGCACAGAA TCAACAAGAT ATGCAGTTCA ACTAATCAAT 
GCACTCATTC AAGATCCTGC TAAGGAACTG GAAGACTTGA TTCCTAAAAA TCATATCAGA 
ACACCTGCCA GCACCAAATC AATTCATGCT AACTTCTCAT CTGGAGTAGG TACCACAGCA 
GCTTCCAGTA AAAATGCATT TCCTTTGGGT GCTCCAACTC TTGTAACTTC ACAGGCAACA 
ACGTTATCTA CGTTCCAGCC CGCTAATAAA CTTAATAAGA ATGTTCCAAC AAATGTACGT 
TCTTCTTTCC CAGTTTCTCT ACCCTTAGCT TATCCTCACC CTCATTTTGC CCTGCTGGCT 
GCTCAAACTA TGCAACAGAT TCGGCATCCT CGCTTACCCA TGGCCCAGTT TGGAGGAACC 
TTCTCACCTT CTCCTAACAC ATGGGGACCA TTCCCAGTGA GACCTGTGAA TCCTGGCAAC 
ACAAATAGCT CTCCAAAGCA TAATAACACA AGCCGTCTAC CTAACCAGAA CGGGACTGTT 
TTACCCTCAG AGTCTGCTGG ACTAGCTACT GCCAGTTGTC CTATCACTGT CTCTTCTGTA 
GTTGCTGCCA GTCAGCAACT GTGTGTCACT AATACCCGGA CTCCTTCATC AGTCAGAAAG 
CAGTTGTTTG CCTGTGTGCC TAAGACAAGT CCTCCAGCAA CAGTGATTTC TTCTGTGACA 
AGCACTTGTA GTTCCCTGCC TTCTGTCTCC TCTGCACCTA TCACTAGCGG GCAAGCTCCC 
ACCACATTTC TACCTGCAAG TACTTCTCAA GCACAGCTTT CTTCACAAAA GATGGAGTCT 
TTCTCTGCTG TGCCACCCAC CAAAGAGAAA GTGTCCACAC AGGACCAGCC CATGGCAAAC 
CTATGTACCC CATCTTCAAC TGCAAACAGT TGCAGTAGCT CTGCCAGCAA CACCCCGGGA 
GCTCCAGAAA CTCACCCATC CAGTAGTCCC ACTCCTACTT CCAGTAACAC ACAAGAGGAG 
GCACAGCCAT CCAGTGTGTC TGATTTAAGT CCTATGTCAA TGCCTTTTGC ATCTAACTCA 
GAACCTGCTC CATTGACTTT GACATCACCC AGAATGGTTG CTGCTGATAA TCAGGACACC 
AGTAATTTAC CTCAGTTAGC TGTACCAGCA CCTCGAGTTT CTCATCGAAT GCAGCCCAGA 
GGTTCTTTTT ACTCCATGGT ACCAAATGCA ACTATTCACC AGGATCCCCA GTCTATTTTT 
GTTACGAATC CAGTTACTTT AACACCACCT CAAGGCCCAC CAGCTGCAGT GCAGCTTTCT 
TCAGCTGTGA ACATTATGAA TGGTTCTCAG ATGCACATAA ACCCAGCAAA TAAGTCTTTG 
CCACCTACAT TTGGCCCAGC CACACTTTTC AATCACTTCA GCAGTCTTTT TGATAGTAGT 
CAGGTGCCAG CTAACCAGGG CTGGGGAGAT GGTCCACTGT CCTCACGAGT TGCTACAGAT 
GCCTCTTTCA CTGTTCAGTC AGCGTTCCTG GGTAACTCAG TGCTTGGACA CTTGGAAAAC 
ATGCACCCTG ATAACTCAAA GGCACCTGGC TTCAGACCAC CTTCCCAGCG AGTTTCTACT 
AGTCCAGTTG GGTTACCATC CATTGACCCA TCAGGCAGCT CCCCATCTTC CTCTTCTGCT 
CCTCTGGCAA GTTTTTCCGG CATACCAGGA ACAAGGGTTT TCCTGCAAGG GCCAGCTCCT 
GTTGGGACTC CTAGTTTCAA CAGACAACAT TTTTCTCCCC ATCCTTGGAC AAGCGCCTCA 
AACTCATCCA CTTCTGCCCC ACCAACGTTG GGCCAACCAA AAGGAGTCAG TGCCAGTCAA 
GATCGAAAGA TACCTCCCCC AATTGGAACA GAGAGACTGG CCCGAATTCG GCAAGGAGGG 
TCTGTTGCAC AAGCCCCGGC GGGGACCAGT TTTGTCGCTC CCGTTGGACA CAGTGGAATC 
TGGTCATTTG GTGTCAATGC TGTGTCAGAA GGCTTATCAG GTTGGTCGCA ATCTGTGATG 
GGGAACCATC CAATGCATCA ACAATTATCA GACCCAAGCA CATTCTCCCA ACATCAGCCA 
ATGGAGAGAG ATGATTCTGG AATGGTAGCC CCCTCTAACA TTTTTCATCA GCCTATGGCA 
AGTGGTTTTG TGGATTTTTC TAAAGGTCTG CCAATTTCCA TGTATGGAGG CACCATAATA 
CCCTCTCATC CTCAGCTTGC TGATGTTCCA GGAGGCCCTC TGTTTAATGG ACTTCACAAT 
CCAGATCCTG CTTGGAACCC TATGATAAAA GTTATCCAAA ATTCAACTGA ATGCACTGAT 
GCCCAGCAGA TTTGGCCTGG CACGTGGGCA CCTCATATTG GAAACATGCA TCTCAAATAT 
GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKHD1 ( NM_017747.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060217.1 Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 1, mRNA.

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CloneID	OHu02533
Clone ID Related Accession (Same CDS sequence)	NM_017747.2 , NM_017747.3
Accession Version	NM_017747.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and KH domain-containing protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA509209.1, AF521882.1 and AK000904.1. On Nov 22, 2018 this sequence version replaced NM_017747.2. Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF521882.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000360839.7/ ENSP00000354085.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG GAATTGGCAA ATTGTCAACT 
GCTGATGGTA AAGCTTTTGC AGATCCTGAG GTACTCCGGA GACTGACATC CTCAGTTAGT 
TGTGCACTGG ATGAAGCTGC TGCTGCACTG ACACGGATGA AAGCAGAAAA CAGCCACAAT 
GCAGGACAAG TGGACACTCG CAGTCTAGCA GAAGCTTGTT CAGATGGGGA TGTTAATGCT 
GTTCGTAAAT TGCTAGATGA AGGCAGAAGT GTAAATGAAC ATACAGAAGA AGGAGAAAGC 
CTGCTGTGTT TGGCTTGTTC AGCAGGGTAT TATGAATTAG CACAAGTATT GCTTGCTATG 
CATGCTAATG TTGAAGATCG AGGGAATAAA GGAGACATAA CTCCCCTGAT GGCAGCTTCC 
AGTGGAGGTT ACTTAGATAT TGTGAAATTA TTACTTCTTC ATGATGCTGA TGTCAACTCC 
CAGTCTGCAA CAGGAAACAC TGCGCTAACT TATGCATGTG CTGGAGGATT TGTTGACATT 
GTTAAAGTGC TCCTTAATGA AGGTGCAAAT ATAGAAGATC ATAATGAAAA TGGACATACT 
CCCTTAATGG AAGCAGCCAG TGCAGGTCAT GTGGAAGTTG CAAGAGTTCT TTTAGATCAT 
GGTGCAGGCA TCAACACTCA TTCTAATGAA TTCAAAGAAA GTGCTCTAAC ACTTGCTTGC 
TACAAAGGCC ATTTGGATAT GGTTCGCTTT CTACTTGAAG CTGGTGCAGA TCAAGAGCAC 
AAAACAGATG AGATGCACAC TGCCTTAATG GAGGCCTGCA TGGATGGACA TGTAGAGGTG 
GCACGTTTGC TTTTGGATAG TGGTGCTCAA GTGAACATGC CTGCAGATTC ATTTGAATCT 
CCATTGACGC TAGCTGCCTG TGGAGGACAT GTTGAATTGG CAGCTCTACT TATTGAAAGG 
GGAGCAAATC TTGAAGAAGT TAATGATGAA GGATACACTC CCTTGATGGA AGCTGCCCGG 
GAAGGACATG AAGAAATGGT GGCACTACTC TTAGCACAAG GAGCAAATAT AAATGCCCAG 
ACAGAAGAAA CTCAAGAAAC TGCTCTTACT TTGGCTTGCT GTGGAGGATT TTCTGAAGTT 
GCAGACTTTC TTATTAAGGC AGGGGCTGAT ATAGAACTTG GCTGCTCCAC ACCTCTGATG 
GAGGCATCTC AGGAGGGACA CCTGGAATTG GTTAAATATT TGCTGGCTTC TGGCGCTAAT 
GTGCATGCTA CAACAGCAAC AGGAGACACA GCCTTAACCT ATGCTTGTGA AAATGGACAT 
ACGGATGTTG CAGATGTTTT ACTTCAAGCA GGGGCTGATT TAGAACATGA ATCTGAAGGT 
GGAAGAACAC CTTTGATGAA AGCTGCAAGA GCTGGTCATT TGTGCACTGT GCAGTTTCTT 
ATTAGCAAAG GTGCCAATGT TAACAGGGCT ACAGCCAATA ATGATCATAC AGTAGTGTCG 
CTGGCATGTG CAGGAGGCCA CCTGGCAGTT GTTGAGCTTC TCTTGGCTCA TGGGGCTGAC 
CCTACTCATC GACTCAAGGA TGGTTCAACA ATGCTCATTG AAGCTGCAAA GGGTGGCCAT 
ACTAATGTAG TTTCTTATCT GTTGGATTAT CCAAATAATG TTCTGTCAGT TCCCACCACA 
GATGTGTCTC AGCTCCCTCC ACCTTCTCAA GATCAGTCTC AGGTGCCACG TGTGCCAACG 
CATACACTTG CCATGGTTGT ACCTCCCCAG GAACCTGACA GAACTTCACA GGAGAACTCT 
CCTGCCCTTT TAGGAGTGCA AAAAGGTACA TCCAAGCAGA AGTCCAGTTC CCTCCAGGTA 
GCAGATCAGG ACCTACTGCC ATCTTTTCAC CCATACCAGC CTTTGGAGTG CATAGTAGAG 
GAGACTGAAG GCAAGCTGAA TGAACTGGGA CAAAGAATTA GTGCTATTGA AAAAGCACAG 
CTTAAGTCAC TGGAGTTAAT TCAAGGTGAA CCTCTGAACA AAGATAAGAT AGAAGAACTT 
AAAAAGAACA GAGAAGAGCA AGTCCAGAAG AAGAAGAAAA TATTGAAAGA ACTGCAGAAA 
GTGGAAAGGC AGTTGCAGAT GAAAACACAG CAGCAATTTA CCAAAGAATA CTTGGAAACC 
AAAGGTCAGA AAGACACAGT GTCTCTACAC CAACAGTGCT CTCATAGAGG AGTCTTCCCA 
GAAGGGGAAG GAGATGGTAG TCTCCCAGAG GATCACTTTT CAGAGTTACC TCAGGTTGAC 
ACAATCTTAT TTAAAGATAA TGATGTTGAT GATGAGCAAC AGTCTCCACC ATCGGCAGAA 
CAGATTGATT TTGTCCCAGT CCAGCCTTTA TCATCTCCAC AGTGTAACTT TTCCAGTGAC 
TTAGGTTCTA ATGGGACAAA TTCTCTTGAA CTTCAGAAAG TATCAGGTAA TCAGCAGATT 
GTAGGACAGC CTCAGATTGC TATTACTGGA CATGATCAGG GGCTGTTAGT TCAAGAACCA 
GATGGACTAA TGGTTGCAAC TCCAGCTCAG ACGCTTACCG ACACTCTTGA TGACCTGATA 
GCAGCTGTGA GTACCAGAGT GCCCACTGGT TCCAACAGTT CTTCTCAGAC CACAGAGTGT 
CTTACACCTG AATCCTGTTC GCAGACTACA AGCAATGTGG CTTCCCAATC GATGCCTCCT 
GTGTATCCTT CAGTTGACAT TGATGCACAT ACTGAGAGCA ATCATGACAC AGCATTAACA 
CTAGCTTGTG CAGGTGGTCA TGAAGAACTT GTATCTGTGC TCATTGCACG GGATGCCAAA 
ATTGAACACA GAGACAAAAA AGGTTTCACA CCACTAATCC TGGCAGCAAC AGCAGGGCAT 
GTTGGAGTTG TTGAAATCCT TTTGGATAAA GGTGGAGATA TAGAAGCACA GTCTGAACGA 
ACTAAGGATA CTCCGCTTTC ATTGGCATGT TCTGGTGGAC GTCAGGAGGT GGTAGACTTG 
CTGCTGGCTC GAGGTGCAAA TAAAGAACAT AGGAACGTAT CTGATTATAC ACCACTGAGT 
CTAGCTGCGT CTGGAGGATA TGTTAATATC ATTAAGATTC TGCTTAATGC TGGGGCAGAA 
ATTAATTCAA GGACTGGGAG TAAACTAGGT ATTTCTCCCC TGATGTTGGC TGCAATGAAT 
GGACATGTTC CTGCAGTAAA ATTGCTGCTC GATATGGGTT CAGACATTAA TGCCCAAATA 
GAGACCAATC GGAACACGGC TCTCACCCTG GCCTGTTTCC AGGGCCGAGC AGAAGTAGTG 
AGTTTGCTTC TGGACCGAAA AGCCAATGTT GAACATAGGG CAAAGACGGG TCTTACCCCC 
TTGATGGAAG CAGCTTCTGG AGGGTATGCA GAGGTTGGAA GAGTTCTTCT TGATAAAGGA 
GCAGATGTTA ATGCTCCCCC TGTGCCTTCC TCAAGAGATA CTGCTTTAAC AATAGCAGCA 
GACAAAGGTC ACTACAAATT TTGTGAACTC CTGATTCATA GGGGAGCCCA CATTGATGTT 
CGTAACAAAA AGGGAAATAC GCCACTTTGG CTGGCATCCA ATGGAGGTCA TTTTGATGTT 
GTGCAGTTGC TAGTGCAAGC AGGTGCTGAT GTGGATGCAG CAGATAACCG GAAAATCACA 
CCTCTTATGT CAGCATTTCG CAAGGGTCAT GTAAAAGTTG TTCAATATTT GGTAAAGGAA 
GTAAATCAGT TCCCTTCTGA TATAGAATGC ATGAGATACA TAGCAACAAT TACAGATAAG 
GAACTGTTGA AAAAATGTCA TCAATGTGTC GAAACCATTG TGAAGGCTAA AGACCAGCAA 
GCTGCAGAAG CAAATAAGAA TGCGAGTATT CTTTTAAAGG AACTTGATCT GGAAAAGTCA 
AGAGAAGAGA GCAGAAAGCA GGCTCTTGCT GCTAAAAGAG AAAAAAGAAA AGAAAAGAGA 
AAAAAGAAAA AAGAGGAACA GAAAAGGAAA CAGGAAGAAG ATGAAGAAAA CAAACCTAAG 
GAGAATTCGG AACTACCAGA GGATGAAGAT GAAGAGGAGA ATGATGAAGA TGTGGAGCAA 
GAAGTTCCCA TAGAACCTCC TAGTGCAACC ACCACCACTA CGATTGGAAT CTCTGCAACA 
TCTGCAACAT TCACAAATGT GTTTGGGAAA AAAAGGGCCA ATGTGGTGAC AACTCCCAGC 
ACCAATCGGA AAAATAAGAA GAACAAAACA AAAGAAACCC CTCCTACAGC ACATTTAATT 
TTACCAGAAC AACATATGTC TTTAGCCCAA CAAAAGGCAG ATAAAAATAA AATAAATGGA 
GAACCTAGAG GTGGTGGTGC AGGTGGGAAT AGTGATTCAG ATAACTTGGA CAGCACAGAC 
TGCAACAGTG AGAGTAGCAG TGGTGGTAAA AGCCAAGAGT TAAATTTTGT GATGGATGTG 
AATTCCTCTA AATACCCCTC ACTGCTCCTT CATTCCCAAG AAGAAAAGAC AAGTACTGCT 
ACTTCCAAAA CTCAGACACG ACTTGAAGGT GAAGTGACTC CTAATTCCTT GTCAACCAGC 
TACAAGACAG TGTCATTGCC ATTAAGCTCT CCAAACATAA AGCTGAATCT CACTAGCCCT 
AAAAGGGGTC AGAAAAGAGA AGAAGGGTGG AAAGAAGTTG TACGAAGGTC AAAGAAATTG 
TCTGTTCCAG CCTCAGTGGT GTCGAGGATA ATGGGAAGAG GAGGATGCAA CATCACTGCA 
ATACAGGATG TTACTGGTGC CCATATTGAT GTGGATAAAC AAAAAGATAA GAATGGCGAG 
AGAATGATCA CAATAAGGGG TGGCACAGAA TCAACAAGAT ATGCAGTTCA ACTAATCAAT 
GCACTCATTC AAGATCCTGC TAAGGAACTG GAAGACTTGA TTCCTAAAAA TCATATCAGA 
ACACCTGCCA GCACCAAATC AATTCATGCT AACTTCTCAT CTGGAGTAGG TACCACAGCA 
GCTTCCAGTA AAAATGCATT TCCTTTGGGT GCTCCAACTC TTGTAACTTC ACAGGCAACA 
ACGTTATCTA CGTTCCAGCC CGCTAATAAA CTTAATAAGA ATGTTCCAAC AAATGTACGT 
TCTTCTTTCC CAGTTTCTCT ACCCTTAGCT TATCCTCACC CTCATTTTGC CCTGCTGGCT 
GCTCAAACTA TGCAACAGAT TCGGCATCCT CGCTTACCCA TGGCCCAGTT TGGAGGAACC 
TTCTCACCTT CTCCTAACAC ATGGGGACCA TTCCCAGTGA GACCTGTGAA TCCTGGCAAC 
ACAAATAGCT CTCCAAAGCA TAATAACACA AGCCGTCTAC CTAACCAGAA CGGGACTGTT 
TTACCCTCAG AGTCTGCTGG ACTAGCTACT GCCAGTTGTC CTATCACTGT CTCTTCTGTA 
GTTGCTGCCA GTCAGCAACT GTGTGTCACT AATACCCGGA CTCCTTCATC AGTCAGAAAG 
CAGTTGTTTG CCTGTGTGCC TAAGACAAGT CCTCCAGCAA CAGTGATTTC TTCTGTGACA 
AGCACTTGTA GTTCCCTGCC TTCTGTCTCC TCTGCACCTA TCACTAGCGG GCAAGCTCCC 
ACCACATTTC TACCTGCAAG TACTTCTCAA GCACAGCTTT CTTCACAAAA GATGGAGTCT 
TTCTCTGCTG TGCCACCCAC CAAAGAGAAA GTGTCCACAC AGGACCAGCC CATGGCAAAC 
CTATGTACCC CATCTTCAAC TGCAAACAGT TGCAGTAGCT CTGCCAGCAA CACCCCGGGA 
GCTCCAGAAA CTCACCCATC CAGTAGTCCC ACTCCTACTT CCAGTAACAC ACAAGAGGAG 
GCACAGCCAT CCAGTGTGTC TGATTTAAGT CCTATGTCAA TGCCTTTTGC ATCTAACTCA 
GAACCTGCTC CATTGACTTT GACATCACCC AGAATGGTTG CTGCTGATAA TCAGGACACC 
AGTAATTTAC CTCAGTTAGC TGTACCAGCA CCTCGAGTTT CTCATCGAAT GCAGCCCAGA 
GGTTCTTTTT ACTCCATGGT ACCAAATGCA ACTATTCACC AGGATCCCCA GTCTATTTTT 
GTTACGAATC CAGTTACTTT AACACCACCT CAAGGCCCAC CAGCTGCAGT GCAGCTTTCT 
TCAGCTGTGA ACATTATGAA TGGTTCTCAG ATGCACATAA ACCCAGCAAA TAAGTCTTTG 
CCACCTACAT TTGGCCCAGC CACACTTTTC AATCACTTCA GCAGTCTTTT TGATAGTAGT 
CAGGTGCCAG CTAACCAGGG CTGGGGAGAT GGTCCACTGT CCTCACGAGT TGCTACAGAT 
GCCTCTTTCA CTGTTCAGTC AGCGTTCCTG GGTAACTCAG TGCTTGGACA CTTGGAAAAC 
ATGCACCCTG ATAACTCAAA GGCACCTGGC TTCAGACCAC CTTCCCAGCG AGTTTCTACT 
AGTCCAGTTG GGTTACCATC CATTGACCCA TCAGGCAGCT CCCCATCTTC CTCTTCTGCT 
CCTCTGGCAA GTTTTTCCGG CATACCAGGA ACAAGGGTTT TCCTGCAAGG GCCAGCTCCT 
GTTGGGACTC CTAGTTTCAA CAGACAACAT TTTTCTCCCC ATCCTTGGAC AAGCGCCTCA 
AACTCATCCA CTTCTGCCCC ACCAACGTTG GGCCAACCAA AAGGAGTCAG TGCCAGTCAA 
GATCGAAAGA TACCTCCCCC AATTGGAACA GAGAGACTGG CCCGAATTCG GCAAGGAGGG 
TCTGTTGCAC AAGCCCCGGC GGGGACCAGT TTTGTCGCTC CCGTTGGACA CAGTGGAATC 
TGGTCATTTG GTGTCAATGC TGTGTCAGAA GGCTTATCAG GTTGGTCGCA ATCTGTGATG 
GGGAACCATC CAATGCATCA ACAATTATCA GACCCAAGCA CATTCTCCCA ACATCAGCCA 
ATGGAGAGAG ATGATTCTGG AATGGTAGCC CCCTCTAACA TTTTTCATCA GCCTATGGCA 
AGTGGTTTTG TGGATTTTTC TAAAGGTCTG CCAATTTCCA TGTATGGAGG CACCATAATA 
CCCTCTCATC CTCAGCTTGC TGATGTTCCA GGAGGCCCTC TGTTTAATGG ACTTCACAAT 
CCAGATCCTG CTTGGAACCC TATGATAAAA GTTATCCAAA ATTCAACTGA ATGCACTGAT 
GCCCAGCAGA TTTGGCCTGG CACGTGGGCA CCTCATATTG GAAACATGCA TCTCAAATAT 
GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060217.1
CDS	136..7764
Translation	MLTDSGGGGTSFEEDLDSVAPRSAPAGASEPPPPGGVGLGIRTVRLFGEAGPASGVGSSGGGGSGSGTGGGDAALDFKLAAAVLRTGGGGGASGSDEDEVSEVESFILDQEDLDNPVLKTTSEIFLSSTAEGADLRTVDPETQARLEALLEAAGIGKLSTADGKAFADPEVLRRLTSSVSCALDEAAAALTRMKAENSHNAGQVDTRSLAEACSDGDVNAVRKLLDEGRSVNEHTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGNKGDITPLMAASSGGYLDIVKLLLLHDADVNSQSATGNTALTYACAGGFVDIVKVLLNEGANIEDHNENGHTPLMEAASAGHVEVARVLLDHGAGINTHSNEFKESALTLACYKGHLDMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLDSGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFSEVADFLIKAGADIELGCSTPLMEASQEGHLELVKYLLASGANVHATTATGDTALTYACENGHTDVADVLLQAGADLEHESEGGRTPLMKAARAGHLCTVQFLISKGANVNRATANNDHTVVSLACAGGHLAVVELLLAHGADPTHRLKDGSTMLIEAAKGGHTNVVSYLLDYPNNVLSVPTTDVSQLPPPSQDQSQVPRVPTHTLAMVVPPQEPDRTSQENSPALLGVQKGTSKQKSSSLQVADQDLLPSFHPYQPLECIVEETEGKLNELGQRISAIEKAQLKSLELIQGEPLNKDKIEELKKNREEQVQKKKKILKELQKVERQLQMKTQQQFTKEYLETKGQKDTVSLHQQCSHRGVFPEGEGDGSLPEDHFSELPQVDTILFKDNDVDDEQQSPPSAEQIDFVPVQPLSSPQCNFSSDLGSNGTNSLELQKVSGNQQIVGQPQIAITGHDQGLLVQEPDGLMVATPAQTLTDTLDDLIAAVSTRVPTGSNSSSQTTECLTPESCSQTTSNVASQSMPPVYPSVDIDAHTESNHDTALTLACAGGHEELVSVLIARDAKIEHRDKKGFTPLILAATAGHVGVVEILLDKGGDIEAQSERTKDTPLSLACSGGRQEVVDLLLARGANKEHRNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHVPAVKLLLDMGSDINAQIETNRNTALTLACFQGRAEVVSLLLDRKANVEHRAKTGLTPLMEAASGGYAEVGRVLLDKGADVNAPPVPSSRDTALTIAADKGHYKFCELLIHRGAHIDVRNKKGNTPLWLASNGGHFDVVQLLVQAGADVDAADNRKITPLMSAFRKGHVKVVQYLVKEVNQFPSDIECMRYIATITDKELLKKCHQCVETIVKAKDQQAAEANKNASILLKELDLEKSREESRKQALAAKREKRKEKRKKKKEEQKRKQEEDEENKPKENSELPEDEDEEENDEDVEQEVPIEPPSATTTTTIGISATSATFTNVFGKKRANVVTTPSTNRKNKKNKTKETPPTAHLILPEQHMSLAQQKADKNKINGEPRGGGAGGNSDSDNLDSTDCNSESSSGGKSQELNFVMDVNSSKYPSLLLHSQEEKTSTATSKTQTRLEGEVTPNSLSTSYKTVSLPLSSPNIKLNLTSPKRGQKREEGWKEVVRRSKKLSVPASVVSRIMGRGGCNITAIQDVTGAHIDVDKQKDKNGERMITIRGGTESTRYAVQLINALIQDPAKELEDLIPKNHIRTPASTKSIHANFSSGVGTTAASSKNAFPLGAPTLVTSQATTLSTFQPANKLNKNVPTNVRSSFPVSLPLAYPHPHFALLAAQTMQQIRHPRLPMAQFGGTFSPSPNTWGPFPVRPVNPGNTNSSPKHNNTSRLPNQNGTVLPSESAGLATASCPITVSSVVAASQQLCVTNTRTPSSVRKQLFACVPKTSPPATVISSVTSTCSSLPSVSSAPITSGQAPTTFLPASTSQAQLSSQKMESFSAVPPTKEKVSTQDQPMANLCTPSSTANSCSSSASNTPGAPETHPSSSPTPTSSNTQEEAQPSSVSDLSPMSMPFASNSEPAPLTLTSPRMVAADNQDTSNLPQLAVPAPRVSHRMQPRGSFYSMVPNATIHQDPQSIFVTNPVTLTPPQGPPAAVQLSSAVNIMNGSQMHINPANKSLPPTFGPATLFNHFSSLFDSSQVPANQGWGDGPLSSRVATDASFTVQSAFLGNSVLGHLENMHPDNSKAPGFRPPSQRVSTSPVGLPSIDPSGSSPSSSSAPLASFSGIPGTRVFLQGPAPVGTPSFNRQHFSPHPWTSASNSSTSAPPTLGQPKGVSASQDRKIPPPIGTERLARIRQGGSVAQAPAGTSFVAPVGHSGIWSFGVNAVSEGLSGWSQSVMGNHPMHQQLSDPSTFSQHQPMERDDSGMVAPSNIFHQPMASGFVDFSKGLPISMYGGTIIPSHPQLADVPGGPLFNGLHNPDPAWNPMIKVIQNSTECTDAQQIWPGTWAPHIGNMHLKYVN

Target ORF information:

RefSeq Version	NM_017747.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017747.3

ORF Insert Sequence:

ATGCTGACTG ATAGCGGAGG CGGCGGCACC TCCTTTGAGG AGGACCTGGA CTCTGTGGCT 
CCGCGATCCG CCCCAGCTGG GGCCTCGGAG CCGCCTCCGC CGGGAGGGGT CGGTCTGGGG 
ATCCGCACCG TGAGGCTCTT TGGGGAGGCC GGGCCAGCGT CGGGAGTCGG CAGCAGCGGC 
GGCGGCGGCA GCGGCAGCGG TACGGGCGGA GGGGACGCGG CGCTGGATTT CAAGTTGGCG 
GCTGCCGTGC TGAGGACCGG GGGTGGAGGT GGTGCCTCTG GCAGTGACGA GGACGAAGTG 
TCCGAGGTTG AATCATTTAT TTTGGACCAA GAAGATCTGG ATAACCCAGT GCTTAAAACA 
ACATCAGAGA TATTCTTATC AAGTACTGCA GAAGGAGCAG ACTTACGCAC TGTGGATCCA 
GAGACACAGG CACGACTAGA AGCATTGCTA GAAGCAGCAG GAATTGGCAA ATTGTCAACT 
GCTGATGGTA AAGCTTTTGC AGATCCTGAG GTACTCCGGA GACTGACATC CTCAGTTAGT 
TGTGCACTGG ATGAAGCTGC TGCTGCACTG ACACGGATGA AAGCAGAAAA CAGCCACAAT 
GCAGGACAAG TGGACACTCG CAGTCTAGCA GAAGCTTGTT CAGATGGGGA TGTTAATGCT 
GTTCGTAAAT TGCTAGATGA AGGCAGAAGT GTAAATGAAC ATACAGAAGA AGGAGAAAGC 
CTGCTGTGTT TGGCTTGTTC AGCAGGGTAT TATGAATTAG CACAAGTATT GCTTGCTATG 
CATGCTAATG TTGAAGATCG AGGGAATAAA GGAGACATAA CTCCCCTGAT GGCAGCTTCC 
AGTGGAGGTT ACTTAGATAT TGTGAAATTA TTACTTCTTC ATGATGCTGA TGTCAACTCC 
CAGTCTGCAA CAGGAAACAC TGCGCTAACT TATGCATGTG CTGGAGGATT TGTTGACATT 
GTTAAAGTGC TCCTTAATGA AGGTGCAAAT ATAGAAGATC ATAATGAAAA TGGACATACT 
CCCTTAATGG AAGCAGCCAG TGCAGGTCAT GTGGAAGTTG CAAGAGTTCT TTTAGATCAT 
GGTGCAGGCA TCAACACTCA TTCTAATGAA TTCAAAGAAA GTGCTCTAAC ACTTGCTTGC 
TACAAAGGCC ATTTGGATAT GGTTCGCTTT CTACTTGAAG CTGGTGCAGA TCAAGAGCAC 
AAAACAGATG AGATGCACAC TGCCTTAATG GAGGCCTGCA TGGATGGACA TGTAGAGGTG 
GCACGTTTGC TTTTGGATAG TGGTGCTCAA GTGAACATGC CTGCAGATTC ATTTGAATCT 
CCATTGACGC TAGCTGCCTG TGGAGGACAT GTTGAATTGG CAGCTCTACT TATTGAAAGG 
GGAGCAAATC TTGAAGAAGT TAATGATGAA GGATACACTC CCTTGATGGA AGCTGCCCGG 
GAAGGACATG AAGAAATGGT GGCACTACTC TTAGCACAAG GAGCAAATAT AAATGCCCAG 
ACAGAAGAAA CTCAAGAAAC TGCTCTTACT TTGGCTTGCT GTGGAGGATT TTCTGAAGTT 
GCAGACTTTC TTATTAAGGC AGGGGCTGAT ATAGAACTTG GCTGCTCCAC ACCTCTGATG 
GAGGCATCTC AGGAGGGACA CCTGGAATTG GTTAAATATT TGCTGGCTTC TGGCGCTAAT 
GTGCATGCTA CAACAGCAAC AGGAGACACA GCCTTAACCT ATGCTTGTGA AAATGGACAT 
ACGGATGTTG CAGATGTTTT ACTTCAAGCA GGGGCTGATT TAGAACATGA ATCTGAAGGT 
GGAAGAACAC CTTTGATGAA AGCTGCAAGA GCTGGTCATT TGTGCACTGT GCAGTTTCTT 
ATTAGCAAAG GTGCCAATGT TAACAGGGCT ACAGCCAATA ATGATCATAC AGTAGTGTCG 
CTGGCATGTG CAGGAGGCCA CCTGGCAGTT GTTGAGCTTC TCTTGGCTCA TGGGGCTGAC 
CCTACTCATC GACTCAAGGA TGGTTCAACA ATGCTCATTG AAGCTGCAAA GGGTGGCCAT 
ACTAATGTAG TTTCTTATCT GTTGGATTAT CCAAATAATG TTCTGTCAGT TCCCACCACA 
GATGTGTCTC AGCTCCCTCC ACCTTCTCAA GATCAGTCTC AGGTGCCACG TGTGCCAACG 
CATACACTTG CCATGGTTGT ACCTCCCCAG GAACCTGACA GAACTTCACA GGAGAACTCT 
CCTGCCCTTT TAGGAGTGCA AAAAGGTACA TCCAAGCAGA AGTCCAGTTC CCTCCAGGTA 
GCAGATCAGG ACCTACTGCC ATCTTTTCAC CCATACCAGC CTTTGGAGTG CATAGTAGAG 
GAGACTGAAG GCAAGCTGAA TGAACTGGGA CAAAGAATTA GTGCTATTGA AAAAGCACAG 
CTTAAGTCAC TGGAGTTAAT TCAAGGTGAA CCTCTGAACA AAGATAAGAT AGAAGAACTT 
AAAAAGAACA GAGAAGAGCA AGTCCAGAAG AAGAAGAAAA TATTGAAAGA ACTGCAGAAA 
GTGGAAAGGC AGTTGCAGAT GAAAACACAG CAGCAATTTA CCAAAGAATA CTTGGAAACC 
AAAGGTCAGA AAGACACAGT GTCTCTACAC CAACAGTGCT CTCATAGAGG AGTCTTCCCA 
GAAGGGGAAG GAGATGGTAG TCTCCCAGAG GATCACTTTT CAGAGTTACC TCAGGTTGAC 
ACAATCTTAT TTAAAGATAA TGATGTTGAT GATGAGCAAC AGTCTCCACC ATCGGCAGAA 
CAGATTGATT TTGTCCCAGT CCAGCCTTTA TCATCTCCAC AGTGTAACTT TTCCAGTGAC 
TTAGGTTCTA ATGGGACAAA TTCTCTTGAA CTTCAGAAAG TATCAGGTAA TCAGCAGATT 
GTAGGACAGC CTCAGATTGC TATTACTGGA CATGATCAGG GGCTGTTAGT TCAAGAACCA 
GATGGACTAA TGGTTGCAAC TCCAGCTCAG ACGCTTACCG ACACTCTTGA TGACCTGATA 
GCAGCTGTGA GTACCAGAGT GCCCACTGGT TCCAACAGTT CTTCTCAGAC CACAGAGTGT 
CTTACACCTG AATCCTGTTC GCAGACTACA AGCAATGTGG CTTCCCAATC GATGCCTCCT 
GTGTATCCTT CAGTTGACAT TGATGCACAT ACTGAGAGCA ATCATGACAC AGCATTAACA 
CTAGCTTGTG CAGGTGGTCA TGAAGAACTT GTATCTGTGC TCATTGCACG GGATGCCAAA 
ATTGAACACA GAGACAAAAA AGGTTTCACA CCACTAATCC TGGCAGCAAC AGCAGGGCAT 
GTTGGAGTTG TTGAAATCCT TTTGGATAAA GGTGGAGATA TAGAAGCACA GTCTGAACGA 
ACTAAGGATA CTCCGCTTTC ATTGGCATGT TCTGGTGGAC GTCAGGAGGT GGTAGACTTG 
CTGCTGGCTC GAGGTGCAAA TAAAGAACAT AGGAACGTAT CTGATTATAC ACCACTGAGT 
CTAGCTGCGT CTGGAGGATA TGTTAATATC ATTAAGATTC TGCTTAATGC TGGGGCAGAA 
ATTAATTCAA GGACTGGGAG TAAACTAGGT ATTTCTCCCC TGATGTTGGC TGCAATGAAT 
GGACATGTTC CTGCAGTAAA ATTGCTGCTC GATATGGGTT CAGACATTAA TGCCCAAATA 
GAGACCAATC GGAACACGGC TCTCACCCTG GCCTGTTTCC AGGGCCGAGC AGAAGTAGTG 
AGTTTGCTTC TGGACCGAAA AGCCAATGTT GAACATAGGG CAAAGACGGG TCTTACCCCC 
TTGATGGAAG CAGCTTCTGG AGGGTATGCA GAGGTTGGAA GAGTTCTTCT TGATAAAGGA 
GCAGATGTTA ATGCTCCCCC TGTGCCTTCC TCAAGAGATA CTGCTTTAAC AATAGCAGCA 
GACAAAGGTC ACTACAAATT TTGTGAACTC CTGATTCATA GGGGAGCCCA CATTGATGTT 
CGTAACAAAA AGGGAAATAC GCCACTTTGG CTGGCATCCA ATGGAGGTCA TTTTGATGTT 
GTGCAGTTGC TAGTGCAAGC AGGTGCTGAT GTGGATGCAG CAGATAACCG GAAAATCACA 
CCTCTTATGT CAGCATTTCG CAAGGGTCAT GTAAAAGTTG TTCAATATTT GGTAAAGGAA 
GTAAATCAGT TCCCTTCTGA TATAGAATGC ATGAGATACA TAGCAACAAT TACAGATAAG 
GAACTGTTGA AAAAATGTCA TCAATGTGTC GAAACCATTG TGAAGGCTAA AGACCAGCAA 
GCTGCAGAAG CAAATAAGAA TGCGAGTATT CTTTTAAAGG AACTTGATCT GGAAAAGTCA 
AGAGAAGAGA GCAGAAAGCA GGCTCTTGCT GCTAAAAGAG AAAAAAGAAA AGAAAAGAGA 
AAAAAGAAAA AAGAGGAACA GAAAAGGAAA CAGGAAGAAG ATGAAGAAAA CAAACCTAAG 
GAGAATTCGG AACTACCAGA GGATGAAGAT GAAGAGGAGA ATGATGAAGA TGTGGAGCAA 
GAAGTTCCCA TAGAACCTCC TAGTGCAACC ACCACCACTA CGATTGGAAT CTCTGCAACA 
TCTGCAACAT TCACAAATGT GTTTGGGAAA AAAAGGGCCA ATGTGGTGAC AACTCCCAGC 
ACCAATCGGA AAAATAAGAA GAACAAAACA AAAGAAACCC CTCCTACAGC ACATTTAATT 
TTACCAGAAC AACATATGTC TTTAGCCCAA CAAAAGGCAG ATAAAAATAA AATAAATGGA 
GAACCTAGAG GTGGTGGTGC AGGTGGGAAT AGTGATTCAG ATAACTTGGA CAGCACAGAC 
TGCAACAGTG AGAGTAGCAG TGGTGGTAAA AGCCAAGAGT TAAATTTTGT GATGGATGTG 
AATTCCTCTA AATACCCCTC ACTGCTCCTT CATTCCCAAG AAGAAAAGAC AAGTACTGCT 
ACTTCCAAAA CTCAGACACG ACTTGAAGGT GAAGTGACTC CTAATTCCTT GTCAACCAGC 
TACAAGACAG TGTCATTGCC ATTAAGCTCT CCAAACATAA AGCTGAATCT CACTAGCCCT 
AAAAGGGGTC AGAAAAGAGA AGAAGGGTGG AAAGAAGTTG TACGAAGGTC AAAGAAATTG 
TCTGTTCCAG CCTCAGTGGT GTCGAGGATA ATGGGAAGAG GAGGATGCAA CATCACTGCA 
ATACAGGATG TTACTGGTGC CCATATTGAT GTGGATAAAC AAAAAGATAA GAATGGCGAG 
AGAATGATCA CAATAAGGGG TGGCACAGAA TCAACAAGAT ATGCAGTTCA ACTAATCAAT 
GCACTCATTC AAGATCCTGC TAAGGAACTG GAAGACTTGA TTCCTAAAAA TCATATCAGA 
ACACCTGCCA GCACCAAATC AATTCATGCT AACTTCTCAT CTGGAGTAGG TACCACAGCA 
GCTTCCAGTA AAAATGCATT TCCTTTGGGT GCTCCAACTC TTGTAACTTC ACAGGCAACA 
ACGTTATCTA CGTTCCAGCC CGCTAATAAA CTTAATAAGA ATGTTCCAAC AAATGTACGT 
TCTTCTTTCC CAGTTTCTCT ACCCTTAGCT TATCCTCACC CTCATTTTGC CCTGCTGGCT 
GCTCAAACTA TGCAACAGAT TCGGCATCCT CGCTTACCCA TGGCCCAGTT TGGAGGAACC 
TTCTCACCTT CTCCTAACAC ATGGGGACCA TTCCCAGTGA GACCTGTGAA TCCTGGCAAC 
ACAAATAGCT CTCCAAAGCA TAATAACACA AGCCGTCTAC CTAACCAGAA CGGGACTGTT 
TTACCCTCAG AGTCTGCTGG ACTAGCTACT GCCAGTTGTC CTATCACTGT CTCTTCTGTA 
GTTGCTGCCA GTCAGCAACT GTGTGTCACT AATACCCGGA CTCCTTCATC AGTCAGAAAG 
CAGTTGTTTG CCTGTGTGCC TAAGACAAGT CCTCCAGCAA CAGTGATTTC TTCTGTGACA 
AGCACTTGTA GTTCCCTGCC TTCTGTCTCC TCTGCACCTA TCACTAGCGG GCAAGCTCCC 
ACCACATTTC TACCTGCAAG TACTTCTCAA GCACAGCTTT CTTCACAAAA GATGGAGTCT 
TTCTCTGCTG TGCCACCCAC CAAAGAGAAA GTGTCCACAC AGGACCAGCC CATGGCAAAC 
CTATGTACCC CATCTTCAAC TGCAAACAGT TGCAGTAGCT CTGCCAGCAA CACCCCGGGA 
GCTCCAGAAA CTCACCCATC CAGTAGTCCC ACTCCTACTT CCAGTAACAC ACAAGAGGAG 
GCACAGCCAT CCAGTGTGTC TGATTTAAGT CCTATGTCAA TGCCTTTTGC ATCTAACTCA 
GAACCTGCTC CATTGACTTT GACATCACCC AGAATGGTTG CTGCTGATAA TCAGGACACC 
AGTAATTTAC CTCAGTTAGC TGTACCAGCA CCTCGAGTTT CTCATCGAAT GCAGCCCAGA 
GGTTCTTTTT ACTCCATGGT ACCAAATGCA ACTATTCACC AGGATCCCCA GTCTATTTTT 
GTTACGAATC CAGTTACTTT AACACCACCT CAAGGCCCAC CAGCTGCAGT GCAGCTTTCT 
TCAGCTGTGA ACATTATGAA TGGTTCTCAG ATGCACATAA ACCCAGCAAA TAAGTCTTTG 
CCACCTACAT TTGGCCCAGC CACACTTTTC AATCACTTCA GCAGTCTTTT TGATAGTAGT 
CAGGTGCCAG CTAACCAGGG CTGGGGAGAT GGTCCACTGT CCTCACGAGT TGCTACAGAT 
GCCTCTTTCA CTGTTCAGTC AGCGTTCCTG GGTAACTCAG TGCTTGGACA CTTGGAAAAC 
ATGCACCCTG ATAACTCAAA GGCACCTGGC TTCAGACCAC CTTCCCAGCG AGTTTCTACT 
AGTCCAGTTG GGTTACCATC CATTGACCCA TCAGGCAGCT CCCCATCTTC CTCTTCTGCT 
CCTCTGGCAA GTTTTTCCGG CATACCAGGA ACAAGGGTTT TCCTGCAAGG GCCAGCTCCT 
GTTGGGACTC CTAGTTTCAA CAGACAACAT TTTTCTCCCC ATCCTTGGAC AAGCGCCTCA 
AACTCATCCA CTTCTGCCCC ACCAACGTTG GGCCAACCAA AAGGAGTCAG TGCCAGTCAA 
GATCGAAAGA TACCTCCCCC AATTGGAACA GAGAGACTGG CCCGAATTCG GCAAGGAGGG 
TCTGTTGCAC AAGCCCCGGC GGGGACCAGT TTTGTCGCTC CCGTTGGACA CAGTGGAATC 
TGGTCATTTG GTGTCAATGC TGTGTCAGAA GGCTTATCAG GTTGGTCGCA ATCTGTGATG 
GGGAACCATC CAATGCATCA ACAATTATCA GACCCAAGCA CATTCTCCCA ACATCAGCCA 
ATGGAGAGAG ATGATTCTGG AATGGTAGCC CCCTCTAACA TTTTTCATCA GCCTATGGCA 
AGTGGTTTTG TGGATTTTTC TAAAGGTCTG CCAATTTCCA TGTATGGAGG CACCATAATA 
CCCTCTCATC CTCAGCTTGC TGATGTTCCA GGAGGCCCTC TGTTTAATGG ACTTCACAAT 
CCAGATCCTG CTTGGAACCC TATGATAAAA GTTATCCAAA ATTCAACTGA ATGCACTGAT 
GCCCAGCAGA TTTGGCCTGG CACGTGGGCA CCTCATATTG GAAACATGCA TCTCAAATAT 
GTCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization and Genetic Analyses of New Genes Coding for NOD2 Interacting Proteins.
PloS one11(11)e0165420(2016)
Thi?baut R,Esmiol S,Lecine P,Mahfouz B,Hermant A,Nicoletti C,Parnis S,Perroy J,Borg JP,Pascoe L,Hugot JP,Ollendorff V

ANKHD1 silencing inhibits Stathmin 1 activity, cell proliferation and migration of leukemia cells.
Biochimica et biophysica acta1853(3)583-93(2015 Mar)
Machado-Neto JA,Lazarini M,Favaro P,de Melo Campos P,Scopim-Ribeiro R,Franchi Junior GC,Nowill AE,Lima PR,Costa FF,Benichou S,Olalla Saad ST,Traina F

ANKHD1 represses p21 (WAF1/CIP1) promoter and promotes multiple myeloma cell growth.
European journal of cancer (Oxford, England : 1990)51(2)252-9(2015 Jan)
Dhyani A,Machado-Neto JA,Favaro P,Saad ST

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ANKHD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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