TRIT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRIT1
Entrez Gene ID	54802
Full Name	tRNA isopentenyltransferase 1
Synonyms	GRO1,IPPT,IPT,IPTase,MOD5,hGRO1
General protein information	Preferred Names tRNA isopentenyltransferase 1 Names tRNA dimethylallyltransferase tRNA dimethylallyltransferase, mitochondrial IPP transferase isopentenyl-diphosphate:tRNA isopentenyltransferase tRNA isopentenylpyrophosphate transferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.2
Summary	This gene encodes a protein that that is targeted to the mitochondrion and modifies transfer RNAs (tRNAs) by adding a dimethylallyl group onto the adenine at position 37. This modification is important for maintaining the correct reading frame during protein translation. This gene is considered a tumor suppressor and its expression can decrease cell growth. Alternative splicing results in multiple transcripts variants, most of which are likely non-functional. [provided by RefSeq, Aug 2015].
Disorder MIM:	617840

mRNA and Protein(s)

mRNA	Protein	Name
NM_017646.5	NP_060116.2	tRNA dimethylallyltransferase isoform 1 precursor
XM_006710706.1	XP_006710769.1	tRNA dimethylallyltransferase isoform X2
NM_001312692.1	NP_001299621.1	tRNA dimethylallyltransferase isoform 3
NM_001312691.1	NP_001299620.1	tRNA dimethylallyltransferase isoform 2
XM_005270954.2	XP_005271011.1	tRNA dimethylallyltransferase isoform X1
NM_017646.6	NP_060116.2	tRNA dimethylallyltransferase isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRIT1	NP_060116.2
Schizosaccharomyces pombe (fission yeast)	tit1	NP_593436.1
Arabidopsis thaliana (thale cress)	IPT2	NP_565658.1
Xenopus tropicalis (tropical clawed frog)	trit1	NP_001011306.1
Canis lupus familiaris (dog)	TRIT1	XP_532548.2
Bos taurus (cattle)	TRIT1	NP_001179769.1
Mus musculus (house mouse)	Trit1	NP_080149.2
Danio rerio (zebrafish)	trit1	NP_001038239.1
Drosophila melanogaster (fruit fly)	CG31381	NP_001189283.1
Magnaporthe oryzae (rice blast fungus)	MGG_04857	XP_003712336.1
Neurospora crassa	NCU10185	XP_958834.2
Gallus gallus (chicken)	TRIT1	XP_004947943.1
Caenorhabditis elegans (roundworm)	gro-1	NP_498122.2
Saccharomyces cerevisiae (baker's yeast)	MOD5	NP_014917.3
Eremothecium gossypii	AGOS_AER341W	NP_985197.1
Pan troglodytes (chimpanzee)	TRIT1	XP_513347.2
	TRIT1	XP_001113925.1
Rattus norvegicus (Norway rat)	Trit1	NP_001102146.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000639	XP_311166.5
Kluyveromyces lactis	KLLA0C07359g	XP_452527.1
Oryza sativa (rice)	Os01g0968700	NP_001045520.1

Gene Ontology
Bibliography

Related articles in PubMed

Matchmaking facilitates the diagnosis of an autosomal-recessive mitochondrial disease caused by biallelic mutation of the tRNA isopentenyltransferase (TRIT1) gene.
Kernohan KD, Dyment DA, Pupavac M, Cramer Z, McBride A, Bernard G, Straub I, Tetreault M, Hartley T, Huang L, Sell E, Majewski J, Rosenblatt DS, Shoubridge E, Mhanni A, Myers T, Proud V, Vergano S, Spangler B, Farrow E, Kussman J, Safina N, , Saunders C, Boycott KM, Thiffault I
Human mutation38(5)511-516(2017 May)

The human tRNA-modifying protein, TRIT1, forms amyloid fibers in vitro.
Waller TJ, Read DF, Engelke DR, Smaldino PJ
Gene61219-24(2017 May)

The cytoplasmic and nuclear populations of the eukaryote tRNA-isopentenyl transferase have distinct functions with implications in human cancer.
Smaldino PJ, Read DF, Pratt-Hyatt M, Hopper AK, Engelke DR
Gene556(1)13-8(2015 Feb)

Defective i6A37 modification of mitochondrial and cytosolic tRNAs results from pathogenic mutations in TRIT1 and its substrate tRNA.
Yarham JW, Lamichhane TN, Pyle A, Mattijssen S, Baruffini E, Bruni F, Donnini C, Vassilev A, He L, Blakely EL, Griffin H, Santibanez-Koref M, Bindoff LA, Ferrero I, Chinnery PF, McFarland R, Maraia RJ, Taylor RW
PLoS genetics10(6)e1004424(2014 Jun)

Human cells have a limited set of tRNA anticodon loop substrates of the tRNA isopentenyltransferase TRIT1 tumor suppressor.
Lamichhane TN, Mattijssen S, Maraia RJ
Molecular and cellular biology33(24)4900-8(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We show that dysfunctional TRIT1 results in decreased levels of select mitochondrial proteins. Our findings confirm the TRIT1 disease association and advance the phenotypic and molecular understanding of this disorder.
Title: Matchmaking facilitates the diagnosis of an autosomal-recessive mitochondrial disease caused by biallelic mutation of the tRNA isopentenyltransferase (TRIT1) gene.

characterize TRIT1 as a novel human amyloid fiber forming protein. We discuss these data in light of TRIT1's functional roles and possible implications for disease
Title: The human tRNA-modifying protein, TRIT1, forms amyloid fibers in vitro.

In light of this additional nuclear role for Mod5 we discuss the proposed role of the human homologue of Mod5, TRIT1, as a tumor suppressor protein.[review]
Title: The cytoplasmic and nuclear populations of the eukaryote tRNA-isopentenyl transferase have distinct functions with implications in human cancer.

patient cells bearing the p.Arg323Gln TRIT1 mutation are severely deficient in i6A37 in both cytosolic and mitochondrial tRNAs
Title: Defective i6A37 modification of mitochondrial and cytosolic tRNAs results from pathogenic mutations in TRIT1 and its substrate tRNA.

TRIT1 may control the levels of some tRNAs as well as their specific activity.
Title: Human cells have a limited set of tRNA anticodon loop substrates of the tRNA isopentenyltransferase TRIT1 tumor suppressor.

The following TRIT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01725	NM_017646.6 Latest version!	Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu68602	NM_001312691.1 Latest version!	Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant 16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu68603	NM_001312692.1 Latest version!	Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu33526	XM_005270954.2 Latest version!	Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu33527	XM_006710706.1 Latest version!	Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu01725	NM_017646.5	Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRIT1 ( NM_017646.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060116.2 Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01725
Clone ID Related Accession (Same CDS sequence)	NM_017646.5 , NM_017646.6
Accession Version	NM_017646.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	tRNA dimethylallyltransferase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE294565.1, AY702933.1 and AK223478.1. This sequence is a reference standard in the RefSeqGene project. On Aug 19, 2015 this sequence version replaced NM_017646.4. Transcript Variant: This variant (1) encodes the longest functional protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AK223478.1, SRR5189655.191625.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCG TGGCGGCTGC ACGAGCAGTT CCCGTGGGCA GTGGGCTCAG GGGCCTGCAA 
CGGACCCTAC CTCTTGTAGT GATTCTCGGG GCCACGGGCA CCGGCAAATC CACGCTGGCG 
TTGCAGCTAG GCCAGCGGCT CGGCGGTGAG ATCGTCAGCG CTGACTCCAT GCAGGTCTAT 
GAAGGCCTAG ACATCATCAC CAACAAGGTT TCTGCCCAAG AGCAGAGAAT CTGCCGGCAC 
CACATGATCA GCTTTGTGGA TCCTCTTGTG ACCAATTACA CAGTGGTGGA CTTCAGAAAT 
AGAGCAACTG CTCTGATTGA AGATATATTT GCCCGAGACA AAATTCCTAT TGTTGTGGGA 
GGAACCAATT ATTACATTGA ATCTCTGCTC TGGAAAGTTC TTGTCAATAC CAAGCCCCAG 
GAGATGGGCA CTGAGAAAGT GATTGACCGA AAAGTGGAGC TTGAAAAGGA GGATGGTCTT 
GTACTTCACA AACGCCTAAG CCAGGTGGAC CCAGAAATGG CTGCCAAGCT GCATCCACAT 
GACAAACGCA AAGTGGCCAG GAGCTTGCAA GTTTTTGAAG AAACAGGAAT CTCTCATAGT 
GAATTTCTCC ATCGTCAACA TACGGAAGAA GGTGGTGGTC CCCTTGGAGG TCCTCTGAAG 
TTCTCTAACC CTTGCATCCT TTGGCTTCAT GCTGACCAGG CAGTTCTAGA TGAGCGCTTG 
GATAAGAGGG TGGATGACAT GCTTGCTGCT GGGCTCTTGG AGGAACTAAG AGATTTTCAC 
AGACGCTATA ATCAGAAGAA TGTTTCGGAA AATAGCCAGG ACTATCAACA TGGTATCTTC 
CAATCAATTG GCTTCAAGGA ATTTCACGAG TACCTGATCA CTGAGGGAAA ATGCACACTG 
GAGACTAGTA ACCAGCTTCT AAAGAAAGGT ATTGAGGCTC TGAAACAAGT AACTAAGAGA 
TATGCCCGGA AACAAAACCG ATGGGTTAAA AACCGTTTTT TGAGCAGACC TGGTCCCATT 
GTCCCCCCTG TCTATGGCTT AGAGGTATCT GATGTCTCGA AGTGGGAAGA GTCTGTTCTT 
GAACCTGCTC TTGAAATCGT GCAAAGTTTC ATCCAGGGCC ACAAGCCTAC AGCCACTCCA 
ATAAAGATGC CATACAATGA AGCTGAGAAC AAGAGAAGTT ATCACCTGTG TGACCTCTGT 
GATCGAATCA TCATTGGGGA TCGCGAATGG GCAGCGCACA TAAAATCCAA ATCCCACTTG 
AACCAACTGA AGAAAAGAAG AAGATTGGAC TCAGATGCTG TCAACACCAT AGAAAGTCAG 
AGTGTTTCCC CAGACCATAA CAAAGAACCT AAAGAGAAGG GATCCCCAGG GCAGAATGAT 
CAAGAGCTGA AATGCAGCGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060116.2
CDS	21..1424
Translation	MASVAAARAVPVGSGLRGLQRTLPLVVILGATGTGKSTLALQLGQRLGGEIVSADSMQVYEGLDIITNKVSAQEQRICRHHMISFVDPLVTNYTVVDFRNRATALIEDIFARDKIPIVVGGTNYYIESLLWKVLVNTKPQEMGTEKVIDRKVELEKEDGLVLHKRLSQVDPEMAAKLHPHDKRKVARSLQVFEETGISHSEFLHRQHTEEGGGPLGGPLKFSNPCILWLHADQAVLDERLDKRVDDMLAAGLLEELRDFHRRYNQKNVSENSQDYQHGIFQSIGFKEFHEYLITEGKCTLETSNQLLKKGIEALKQVTKRYARKQNRWVKNRFLSRPGPIVPPVYGLEVSDVSKWEESVLEPALEIVQSFIQGHKPTATPIKMPYNEAENKRSYHLCDLCDRIIIGDREWAAHIKSKSHLNQLKKRRRLDSDAVNTIESQSVSPDHNKEPKEKGSPGQNDQELKCSV

Target ORF information:

RefSeq Version	NM_017646.5
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017646.5

ORF Insert Sequence:

ATGGCGTCCG TGGCGGCTGC ACGAGCAGTT CCCGTGGGCA GTGGGCTCAG GGGCCTGCAA 
CGGACCCTAC CTCTTGTAGT GATTCTCGGG GCCACGGGCA CCGGCAAATC CACGCTGGCG 
TTGCAGCTAG GCCAGCGGCT CGGCGGTGAG ATCGTCAGCG CTGACTCCAT GCAGGTCTAT 
GAAGGCCTAG ACATCATCAC CAACAAGGTT TCTGCCCAAG AGCAGAGAAT CTGCCGGCAC 
CACATGATCA GCTTTGTGGA TCCTCTTGTG ACCAATTACA CAGTGGTGGA CTTCAGAAAT 
AGAGCAACTG CTCTGATTGA AGATATATTT GCCCGAGACA AAATTCCTAT TGTTGTGGGA 
GGAACCAATT ATTACATTGA ATCTCTGCTC TGGAAAGTTC TTGTCAATAC CAAGCCCCAG 
GAGATGGGCA CTGAGAAAGT GATTGACCGA AAAGTGGAGC TTGAAAAGGA GGATGGTCTT 
GTACTTCACA AACGCCTAAG CCAGGTGGAC CCAGAAATGG CTGCCAAGCT GCATCCACAT 
GACAAACGCA AAGTGGCCAG GAGCTTGCAA GTTTTTGAAG AAACAGGAAT CTCTCATAGT 
GAATTTCTCC ATCGTCAACA TACGGAAGAA GGTGGTGGTC CCCTTGGAGG TCCTCTGAAG 
TTCTCTAACC CTTGCATCCT TTGGCTTCAT GCTGACCAGG CAGTTCTAGA TGAGCGCTTG 
GATAAGAGGG TGGATGACAT GCTTGCTGCT GGGCTCTTGG AGGAACTAAG AGATTTTCAC 
AGACGCTATA ATCAGAAGAA TGTTTCGGAA AATAGCCAGG ACTATCAACA TGGTATCTTC 
CAATCAATTG GCTTCAAGGA ATTTCACGAG TACCTGATCA CTGAGGGAAA ATGCACACTG 
GAGACTAGTA ACCAGCTTCT AAAGAAAGGT ATTGAGGCTC TGAAACAAGT AACTAAGAGA 
TATGCCCGGA AACAAAACCG ATGGGTTAAA AACCGTTTTT TGAGCAGACC TGGTCCCATT 
GTCCCCCCTG TCTATGGCTT AGAGGTATCT GATGTCTCGA AGTGGGAAGA GTCTGTTCTT 
GAACCTGCTC TTGAAATCGT GCAAAGTTTC ATCCAGGGCC ACAAGCCTAC AGCCACTCCA 
ATAAAGATGC CATACAATGA AGCTGAGAAC AAGAGAAGTT ATCACCTGTG TGACCTCTGT 
GATCGAATCA TCATTGGGGA TCGCGAATGG GCAGCGCACA TAAAATCCAA ATCCCACTTG 
AACCAACTGA AGAAAAGAAG AAGATTGGAC TCAGATGCTG TCAACACCAT AGAAAGTCAG 
AGTGTTTCCC CAGACCATAA CAAAGAACCT AAAGAGAAGG GATCCCCAGG GCAGAATGAT 
CAAGAGCTGA AATGCAGCGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIT1 ( NM_017646.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_060116.2 Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01725
Clone ID Related Accession (Same CDS sequence)	NM_017646.5 , NM_017646.6
Accession Version	NM_017646.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	tRNA dimethylallyltransferase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE294565.1, AY702933.1, AK223478.1 and AL033527.26. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_017646.5. Transcript Variant: This variant (1) encodes the longest functional protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: SRR1803617.25765.1, SRR1660805.12771.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000316891.10/ ENSP00000321810.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCG TGGCGGCTGC ACGAGCAGTT CCCGTGGGCA GTGGGCTCAG GGGCCTGCAA 
CGGACCCTAC CTCTTGTAGT GATTCTCGGG GCCACGGGCA CCGGCAAATC CACGCTGGCG 
TTGCAGCTAG GCCAGCGGCT CGGCGGTGAG ATCGTCAGCG CTGACTCCAT GCAGGTCTAT 
GAAGGCCTAG ACATCATCAC CAACAAGGTT TCTGCCCAAG AGCAGAGAAT CTGCCGGCAC 
CACATGATCA GCTTTGTGGA TCCTCTTGTG ACCAATTACA CAGTGGTGGA CTTCAGAAAT 
AGAGCAACTG CTCTGATTGA AGATATATTT GCCCGAGACA AAATTCCTAT TGTTGTGGGA 
GGAACCAATT ATTACATTGA ATCTCTGCTC TGGAAAGTTC TTGTCAATAC CAAGCCCCAG 
GAGATGGGCA CTGAGAAAGT GATTGACCGA AAAGTGGAGC TTGAAAAGGA GGATGGTCTT 
GTACTTCACA AACGCCTAAG CCAGGTGGAC CCAGAAATGG CTGCCAAGCT GCATCCACAT 
GACAAACGCA AAGTGGCCAG GAGCTTGCAA GTTTTTGAAG AAACAGGAAT CTCTCATAGT 
GAATTTCTCC ATCGTCAACA TACGGAAGAA GGTGGTGGTC CCCTTGGAGG TCCTCTGAAG 
TTCTCTAACC CTTGCATCCT TTGGCTTCAT GCTGACCAGG CAGTTCTAGA TGAGCGCTTG 
GATAAGAGGG TGGATGACAT GCTTGCTGCT GGGCTCTTGG AGGAACTAAG AGATTTTCAC 
AGACGCTATA ATCAGAAGAA TGTTTCGGAA AATAGCCAGG ACTATCAACA TGGTATCTTC 
CAATCAATTG GCTTCAAGGA ATTTCACGAG TACCTGATCA CTGAGGGAAA ATGCACACTG 
GAGACTAGTA ACCAGCTTCT AAAGAAAGGT ATTGAGGCTC TGAAACAAGT AACTAAGAGA 
TATGCCCGGA AACAAAACCG ATGGGTTAAA AACCGTTTTT TGAGCAGACC TGGTCCCATT 
GTCCCCCCTG TCTATGGCTT AGAGGTATCT GATGTCTCGA AGTGGGAAGA GTCTGTTCTT 
GAACCTGCTC TTGAAATCGT GCAAAGTTTC ATCCAGGGCC ACAAGCCTAC AGCCACTCCA 
ATAAAGATGC CATACAATGA AGCTGAGAAC AAGAGAAGTT ATCACCTGTG TGACCTCTGT 
GATCGAATCA TCATTGGGGA TCGCGAATGG GCAGCGCACA TAAAATCCAA ATCCCACTTG 
AACCAACTGA AGAAAAGAAG AAGATTGGAC TCAGATGCTG TCAACACCAT AGAAAGTCAG 
AGTGTTTCCC CAGACCATAA CAAAGAACCT AAAGAGAAGG GATCCCCAGG GCAGAATGAT 
CAAGAGCTGA AATGCAGCGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060116.2
CDS	14..1417
Translation	MASVAAARAVPVGSGLRGLQRTLPLVVILGATGTGKSTLALQLGQRLGGEIVSADSMQVYEGLDIITNKVSAQEQRICRHHMISFVDPLVTNYTVVDFRNRATALIEDIFARDKIPIVVGGTNYYIESLLWKVLVNTKPQEMGTEKVIDRKVELEKEDGLVLHKRLSQVDPEMAAKLHPHDKRKVARSLQVFEETGISHSEFLHRQHTEEGGGPLGGPLKFSNPCILWLHADQAVLDERLDKRVDDMLAAGLLEELRDFHRRYNQKNVSENSQDYQHGIFQSIGFKEFHEYLITEGKCTLETSNQLLKKGIEALKQVTKRYARKQNRWVKNRFLSRPGPIVPPVYGLEVSDVSKWEESVLEPALEIVQSFIQGHKPTATPIKMPYNEAENKRSYHLCDLCDRIIIGDREWAAHIKSKSHLNQLKKRRRLDSDAVNTIESQSVSPDHNKEPKEKGSPGQNDQELKCSV

Target ORF information:

RefSeq Version	NM_017646.6
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017646.6

ORF Insert Sequence:

ATGGCGTCCG TGGCGGCTGC ACGAGCAGTT CCCGTGGGCA GTGGGCTCAG GGGCCTGCAA 
CGGACCCTAC CTCTTGTAGT GATTCTCGGG GCCACGGGCA CCGGCAAATC CACGCTGGCG 
TTGCAGCTAG GCCAGCGGCT CGGCGGTGAG ATCGTCAGCG CTGACTCCAT GCAGGTCTAT 
GAAGGCCTAG ACATCATCAC CAACAAGGTT TCTGCCCAAG AGCAGAGAAT CTGCCGGCAC 
CACATGATCA GCTTTGTGGA TCCTCTTGTG ACCAATTACA CAGTGGTGGA CTTCAGAAAT 
AGAGCAACTG CTCTGATTGA AGATATATTT GCCCGAGACA AAATTCCTAT TGTTGTGGGA 
GGAACCAATT ATTACATTGA ATCTCTGCTC TGGAAAGTTC TTGTCAATAC CAAGCCCCAG 
GAGATGGGCA CTGAGAAAGT GATTGACCGA AAAGTGGAGC TTGAAAAGGA GGATGGTCTT 
GTACTTCACA AACGCCTAAG CCAGGTGGAC CCAGAAATGG CTGCCAAGCT GCATCCACAT 
GACAAACGCA AAGTGGCCAG GAGCTTGCAA GTTTTTGAAG AAACAGGAAT CTCTCATAGT 
GAATTTCTCC ATCGTCAACA TACGGAAGAA GGTGGTGGTC CCCTTGGAGG TCCTCTGAAG 
TTCTCTAACC CTTGCATCCT TTGGCTTCAT GCTGACCAGG CAGTTCTAGA TGAGCGCTTG 
GATAAGAGGG TGGATGACAT GCTTGCTGCT GGGCTCTTGG AGGAACTAAG AGATTTTCAC 
AGACGCTATA ATCAGAAGAA TGTTTCGGAA AATAGCCAGG ACTATCAACA TGGTATCTTC 
CAATCAATTG GCTTCAAGGA ATTTCACGAG TACCTGATCA CTGAGGGAAA ATGCACACTG 
GAGACTAGTA ACCAGCTTCT AAAGAAAGGT ATTGAGGCTC TGAAACAAGT AACTAAGAGA 
TATGCCCGGA AACAAAACCG ATGGGTTAAA AACCGTTTTT TGAGCAGACC TGGTCCCATT 
GTCCCCCCTG TCTATGGCTT AGAGGTATCT GATGTCTCGA AGTGGGAAGA GTCTGTTCTT 
GAACCTGCTC TTGAAATCGT GCAAAGTTTC ATCCAGGGCC ACAAGCCTAC AGCCACTCCA 
ATAAAGATGC CATACAATGA AGCTGAGAAC AAGAGAAGTT ATCACCTGTG TGACCTCTGT 
GATCGAATCA TCATTGGGGA TCGCGAATGG GCAGCGCACA TAAAATCCAA ATCCCACTTG 
AACCAACTGA AGAAAAGAAG AAGATTGGAC TCAGATGCTG TCAACACCAT AGAAAGTCAG 
AGTGTTTCCC CAGACCATAA CAAAGAACCT AAAGAGAAGG GATCCCCAGG GCAGAATGAT 
CAAGAGCTGA AATGCAGCGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIT1 ( NM_001312691.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001299620.1 Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant 16, mRNA.

Price & Availability↑

CloneID	OHu68602
Clone ID Related Accession (Same CDS sequence)	NM_001312691.1
Accession Version	NM_001312691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	tRNA dimethylallyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE294565.1, AY052768.1, BC010741.1 and AK223478.1. Transcript Variant: This variant (16) lacks an alternate in-frame exon compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AY052768.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCG TGGCGGCTGC ACGAGCAGTT CCCGTGGGCA GTGGGCTCAG GGGCCTGCAA 
CGGACCCTAC CTCTTGTAGT GATTCTCGGG GCCACGGGCA CCGGCAAATC CACGCTGGCG 
TTGCAGCTAG GCCAGCGGCT CGGCGGTGAG ATCGTCAGCG CTGACTCCAT GCAGGTCTAT 
GAAGGCCTAG ACATCATCAC CAACAAGGTT TCTGCCCAAG AGCAGAGAAT CTGCCGGCAC 
CACATGATCA GCTTTGTGGA TCCTCTTGTG ACCAATTACA CAGTGGTGGA CTTCAGAAAT 
AGAGCAACTG CTCTGATTGA AGATATATTT GCCCGAGACA AAATTCCTAT TGTTGTGGGA 
GGAACCAATT ATTACATTGA ATCTCTGCTC TGGAAAGTTC TTGTCAATAC CAAGCCCCAG 
GAGATGGGCA CTGAGAAAGT GATTGACCGA AAAGTGGAGC TTGAAAAGGA GGATGGTCTT 
GTACTTCACA AACGCCTAAG CCAGGTGGAC CCAGAAATGG CTGCCAAGCT GCATCCACAT 
GACAAACGCA AAGTGGCCAG GAGCTTGCAA GTTTTTGAAG AAACAGGAAT CTCTCATAGT 
GAATTTCTCC ATCGTCAACA TACGGAAGAA GGTGGTGGTC CCCTTGGAGG TCCTCTGAAG 
TTCTCTAACC CTTGCATCCT TTGGCTTCAT GCTGACCAGG CAGTTCTAGA TGAGCGCTTG 
GATAAGAGGG TGGATGACAT GCTTGCTGCT GGGCTCTTGG AGGAACTAAG AGATTTTCAC 
AGACGCTATA ATCAGAAGAA TGTTTCGGAA AATAGCCAGG ACTATCAACA TGGTATCTTC 
CAATCAATTG GCTTCAAGGA ATTTCACGAG TACCTGATCA CTGAGGGAAA ATGCACACTG 
GAGACTAGTA ACCAGCTTCT AAAGAAAGGA CCTGGTCCCA TTGTCCCCCC TGTCTATGGC 
TTAGAGGTAT CTGATGTCTC GAAGTGGGAA GAGTCTGTTC TTGAACCTGC TCTTGAAATC 
GTGCAAAGTT TCATCCAGGG CCACAAGCCT ACAGCCACTC CAATAAAGAT GCCATACAAT 
GAAGCTGAGA ACAAGAGAAG TTATCACCTG TGTGACCTCT GTGATCGAAT CATCATTGGG 
GATCGCGAAT GGGCAGCGCA CATAAAATCC AAATCCCACT TGAACCAACT GAAGAAAAGA 
AGAAGATTGG ACTCAGATGC TGTCAACACC ATAGAAAGTC AGAGTGTTTC CCCAGACCAT 
AACAAAGAAC CTAAAGAGAA GGGATCCCCA GGGCAGAATG ATCAAGAGCT GAAATGCAGC 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299620.1
CDS	21..1346
Translation	MASVAAARAVPVGSGLRGLQRTLPLVVILGATGTGKSTLALQLGQRLGGEIVSADSMQVYEGLDIITNKVSAQEQRICRHHMISFVDPLVTNYTVVDFRNRATALIEDIFARDKIPIVVGGTNYYIESLLWKVLVNTKPQEMGTEKVIDRKVELEKEDGLVLHKRLSQVDPEMAAKLHPHDKRKVARSLQVFEETGISHSEFLHRQHTEEGGGPLGGPLKFSNPCILWLHADQAVLDERLDKRVDDMLAAGLLEELRDFHRRYNQKNVSENSQDYQHGIFQSIGFKEFHEYLITEGKCTLETSNQLLKKGPGPIVPPVYGLEVSDVSKWEESVLEPALEIVQSFIQGHKPTATPIKMPYNEAENKRSYHLCDLCDRIIIGDREWAAHIKSKSHLNQLKKRRRLDSDAVNTIESQSVSPDHNKEPKEKGSPGQNDQELKCSV

Target ORF information:

RefSeq Version	NM_001312691.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant 16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312691.1

ORF Insert Sequence:

ATGGCGTCCG TGGCGGCTGC ACGAGCAGTT CCCGTGGGCA GTGGGCTCAG GGGCCTGCAA 
CGGACCCTAC CTCTTGTAGT GATTCTCGGG GCCACGGGCA CCGGCAAATC CACGCTGGCG 
TTGCAGCTAG GCCAGCGGCT CGGCGGTGAG ATCGTCAGCG CTGACTCCAT GCAGGTCTAT 
GAAGGCCTAG ACATCATCAC CAACAAGGTT TCTGCCCAAG AGCAGAGAAT CTGCCGGCAC 
CACATGATCA GCTTTGTGGA TCCTCTTGTG ACCAATTACA CAGTGGTGGA CTTCAGAAAT 
AGAGCAACTG CTCTGATTGA AGATATATTT GCCCGAGACA AAATTCCTAT TGTTGTGGGA 
GGAACCAATT ATTACATTGA ATCTCTGCTC TGGAAAGTTC TTGTCAATAC CAAGCCCCAG 
GAGATGGGCA CTGAGAAAGT GATTGACCGA AAAGTGGAGC TTGAAAAGGA GGATGGTCTT 
GTACTTCACA AACGCCTAAG CCAGGTGGAC CCAGAAATGG CTGCCAAGCT GCATCCACAT 
GACAAACGCA AAGTGGCCAG GAGCTTGCAA GTTTTTGAAG AAACAGGAAT CTCTCATAGT 
GAATTTCTCC ATCGTCAACA TACGGAAGAA GGTGGTGGTC CCCTTGGAGG TCCTCTGAAG 
TTCTCTAACC CTTGCATCCT TTGGCTTCAT GCTGACCAGG CAGTTCTAGA TGAGCGCTTG 
GATAAGAGGG TGGATGACAT GCTTGCTGCT GGGCTCTTGG AGGAACTAAG AGATTTTCAC 
AGACGCTATA ATCAGAAGAA TGTTTCGGAA AATAGCCAGG ACTATCAACA TGGTATCTTC 
CAATCAATTG GCTTCAAGGA ATTTCACGAG TACCTGATCA CTGAGGGAAA ATGCACACTG 
GAGACTAGTA ACCAGCTTCT AAAGAAAGGA CCTGGTCCCA TTGTCCCCCC TGTCTATGGC 
TTAGAGGTAT CTGATGTCTC GAAGTGGGAA GAGTCTGTTC TTGAACCTGC TCTTGAAATC 
GTGCAAAGTT TCATCCAGGG CCACAAGCCT ACAGCCACTC CAATAAAGAT GCCATACAAT 
GAAGCTGAGA ACAAGAGAAG TTATCACCTG TGTGACCTCT GTGATCGAAT CATCATTGGG 
GATCGCGAAT GGGCAGCGCA CATAAAATCC AAATCCCACT TGAACCAACT GAAGAAAAGA 
AGAAGATTGG ACTCAGATGC TGTCAACACC ATAGAAAGTC AGAGTGTTTC CCCAGACCAT 
AACAAAGAAC CTAAAGAGAA GGGATCCCCA GGGCAGAATG ATCAAGAGCT GAAATGCAGC 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIT1 ( NM_001312692.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001299621.1 Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant 17, mRNA.

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CloneID	OHu68603
Clone ID Related Accession (Same CDS sequence)	NM_001312692.1
Accession Version	NM_001312692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	tRNA dimethylallyltransferase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE294565.1, BC010741.1 and AK223478.1. Transcript Variant: This variant (17) lacks two alternate in-frame exons and uses an alternate in-frame splice site compared to variant 1. The encoded isoform (3) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC010741.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCG TGGCGGCTGC ACGAGCAGTT CCCGTGGGCA GTGGGCTCAG GGGCCTGCAA 
CGGACCCTAC CTCTTGTAGT GATTCTCGGG GCCACGGGCA CCGGCAAATC CACGCTGGCG 
TTGCAGCTAG GCCAGCGGCT CGGCGGTGAG ATCGTCAGCG CTGACTCCAT GCAGCCCCAG 
GAGATGGGCA CTGAGAAAGT GATTGACCGA AAAGTGGAGC TTGAAAAGGA GGATGGTCTT 
GTACTTCACA AACGCCTAAG CCAGGTGGAC CCAGAAATGG CTGCCAAGCT GCATCCACAT 
GACAAACGCA AAGTGGCCAG GAGCTTGCAA GTTTTTGAAG AAACAGGAAT CTCTCATAGT 
GAATTTCTCC ATCGTCAACA TACGGAAGAA GGTGGTGGTC CCCTTGGAGG TCCTCTGAAG 
TTCTCTAACC CTTGCATCCT TTGGCTTCAT GCTGACCAGG CAGATGAGCG CTTGGATAAG 
AGGGTGGATG ACATGCTTGC TGCTGGGCTC TTGGAGGAAC TAAGAGATTT TCACAGACGC 
TATAATCAGA AGAATGTTTC GGAAAATAGC CAGGACTATC AACATGGTAT CTTCCAATCA 
ATTGGCTTCA AGGAATTTCA CGAGTACCTG ATCACTGAGG GAAAATGCAC ACTGGAGACT 
AGTAACCAGC TTCTAAAGAA AGGTATTGAG GCTCTGAAAC AAGTAACTAA GAGATATGCC 
CGGAAACAAA ACCGATGGGT TAAAAACCGT TTTTTGAGCA GACCTGGTCC CATTGTCCCC 
CCTGTCTATG GCTTAGAGGT ATCTGATGTC TCGAAGTGGG AAGAGTCTGT TCTTGAACCT 
GCTCTTGAAA TCGTGCAAAG TTTCATCCAG GGCCACAAGC CTACAGCCAC TCCAATAAAG 
ATGCCATACA ATGAAGCTGA GAACAAGAGA AGTTATCACC TGTGTGACCT CTGTGATCGA 
ATCATCATTG GGGATCGCGA ATGGGCAGCG CACATAAAAT CCAAATCCCA CTTGAACCAA 
CTGAAGAAAA GAAGAAGATT GGACTCAGAT GCTGTCAACA CCATAGAAAG TCAGAGTGTT 
TCCCCAGACC ATAACAAAGA ACCTAAAGAG AAGGGATCCC CAGGGCAGAA TGATCAAGAG 
CTGAAATGCA GCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299621.1
CDS	21..1178
Translation	MASVAAARAVPVGSGLRGLQRTLPLVVILGATGTGKSTLALQLGQRLGGEIVSADSMQPQEMGTEKVIDRKVELEKEDGLVLHKRLSQVDPEMAAKLHPHDKRKVARSLQVFEETGISHSEFLHRQHTEEGGGPLGGPLKFSNPCILWLHADQADERLDKRVDDMLAAGLLEELRDFHRRYNQKNVSENSQDYQHGIFQSIGFKEFHEYLITEGKCTLETSNQLLKKGIEALKQVTKRYARKQNRWVKNRFLSRPGPIVPPVYGLEVSDVSKWEESVLEPALEIVQSFIQGHKPTATPIKMPYNEAENKRSYHLCDLCDRIIIGDREWAAHIKSKSHLNQLKKRRRLDSDAVNTIESQSVSPDHNKEPKEKGSPGQNDQELKCSV

Target ORF information:

RefSeq Version	NM_001312692.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312692.1

ORF Insert Sequence:

ATGGCGTCCG TGGCGGCTGC ACGAGCAGTT CCCGTGGGCA GTGGGCTCAG GGGCCTGCAA 
CGGACCCTAC CTCTTGTAGT GATTCTCGGG GCCACGGGCA CCGGCAAATC CACGCTGGCG 
TTGCAGCTAG GCCAGCGGCT CGGCGGTGAG ATCGTCAGCG CTGACTCCAT GCAGCCCCAG 
GAGATGGGCA CTGAGAAAGT GATTGACCGA AAAGTGGAGC TTGAAAAGGA GGATGGTCTT 
GTACTTCACA AACGCCTAAG CCAGGTGGAC CCAGAAATGG CTGCCAAGCT GCATCCACAT 
GACAAACGCA AAGTGGCCAG GAGCTTGCAA GTTTTTGAAG AAACAGGAAT CTCTCATAGT 
GAATTTCTCC ATCGTCAACA TACGGAAGAA GGTGGTGGTC CCCTTGGAGG TCCTCTGAAG 
TTCTCTAACC CTTGCATCCT TTGGCTTCAT GCTGACCAGG CAGATGAGCG CTTGGATAAG 
AGGGTGGATG ACATGCTTGC TGCTGGGCTC TTGGAGGAAC TAAGAGATTT TCACAGACGC 
TATAATCAGA AGAATGTTTC GGAAAATAGC CAGGACTATC AACATGGTAT CTTCCAATCA 
ATTGGCTTCA AGGAATTTCA CGAGTACCTG ATCACTGAGG GAAAATGCAC ACTGGAGACT 
AGTAACCAGC TTCTAAAGAA AGGTATTGAG GCTCTGAAAC AAGTAACTAA GAGATATGCC 
CGGAAACAAA ACCGATGGGT TAAAAACCGT TTTTTGAGCA GACCTGGTCC CATTGTCCCC 
CCTGTCTATG GCTTAGAGGT ATCTGATGTC TCGAAGTGGG AAGAGTCTGT TCTTGAACCT 
GCTCTTGAAA TCGTGCAAAG TTTCATCCAG GGCCACAAGC CTACAGCCAC TCCAATAAAG 
ATGCCATACA ATGAAGCTGA GAACAAGAGA AGTTATCACC TGTGTGACCT CTGTGATCGA 
ATCATCATTG GGGATCGCGA ATGGGCAGCG CACATAAAAT CCAAATCCCA CTTGAACCAA 
CTGAAGAAAA GAAGAAGATT GGACTCAGAT GCTGTCAACA CCATAGAAAG TCAGAGTGTT 
TCCCCAGACC ATAACAAAGA ACCTAAAGAG AAGGGATCCC CAGGGCAGAA TGATCAAGAG 
CTGAAATGCA GCGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIT1 ( XM_005270954.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005271011.1 Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant X1, mRNA.

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CloneID	OHu33526
Clone ID Related Accession (Same CDS sequence)	XM_005270954.2
Accession Version	XM_005270954.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tRNA dimethylallyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005270954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGCT TTGTGGATCC TCTTGTGACC AATTACACAG TGGTGGACTT CAGAAATAGA 
GCAACTGCTC TGATTGAAGA TATATTTGCC CGAGACAAAA TTCCTATTGT TGTGGGAGGA 
ACCAATTATT ACATTGAATC TCTGCTCTGG AAAGTTCTTG TCAATACCAA GCCCCAGGAG 
ATGGGCACTG AGAAAGTGAT TGACCGAAAA GTGGAGCTTG AAAAGGAGGA TGGTCTTGTA 
CTTCACAAAC GCCTAAGCCA GGTGGACCCA GAAATGGCTG CCAAGCTGCA TCCACATGAC 
AAACGCAAAG TGGCCAGGAG CTTGCAAGTT TTTGAAGAAA CAGGAATCTC TCATAGTGAA 
TTTCTCCATC GTCAACATAC GGAAGAAGGT GGTGGTCCCC TTGGAGGTCC TCTGAAGTTC 
TCTAACCCTT GCATCCTTTG GCTTCATGCT GACCAGGCAG TTCTAGATGA GCGCTTGGAT 
AAGAGGGTGG ATGACATGCT TGCTGCTGGG CTCTTGGAGG AACTAAGAGA TTTTCACAGA 
CGCTATAATC AGAAGAATGT TTCGGAAAAT AGCCAGGACT ATCAACATGG TATCTTCCAA 
TCAATTGGCT TCAAGGAATT TCACGAGTAC CTGATCACTG AGGGAAAATG CACACTGGAG 
ACTAGTAACC AGCTTCTAAA GAAAGGTATT GAGGCTCTGA AACAAGTAAC TAAGAGATAT 
GCCCGGAAAC AAAACCGATG GGTTAAAAAC CGTTTTTTGA GCAGACCTGG TCCCATTGTC 
CCCCCTGTCT ATGGCTTAGA GGTATCTGAT GTCTCGAAGT GGGAAGAGTC TGTTCTTGAA 
CCTGCTCTTG AAATCGTGCA AAGTTTCATC CAGGGCCACA AGCCTACAGC CACTCCAATA 
AAGATGCCAT ACAATGAAGC TGAGAACAAG AGAAGTTATC ACCTGTGTGA CCTCTGTGAT 
CGAATCATCA TTGGGGATCG CGAATGGGCA GCGCACATAA AATCCAAATC CCACTTGAAC 
CAACTGAAGA AAAGAAGAAG ATTGGACTCA GATGCTGTCA ACACCATAGA AAGTCAGAGT 
GTTTCCCCAG ACCATAACAA AGAACCTAAA GAGAAGGGAT CCCCAGGGCA GAATGATCAA 
GAGCTGAAAT GCAGCGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005271011.1
CDS	500..1660
Translation	MISFVDPLVTNYTVVDFRNRATALIEDIFARDKIPIVVGGTNYYIESLLWKVLVNTKPQEMGTEKVIDRKVELEKEDGLVLHKRLSQVDPEMAAKLHPHDKRKVARSLQVFEETGISHSEFLHRQHTEEGGGPLGGPLKFSNPCILWLHADQAVLDERLDKRVDDMLAAGLLEELRDFHRRYNQKNVSENSQDYQHGIFQSIGFKEFHEYLITEGKCTLETSNQLLKKGIEALKQVTKRYARKQNRWVKNRFLSRPGPIVPPVYGLEVSDVSKWEESVLEPALEIVQSFIQGHKPTATPIKMPYNEAENKRSYHLCDLCDRIIIGDREWAAHIKSKSHLNQLKKRRRLDSDAVNTIESQSVSPDHNKEPKEKGSPGQNDQELKCSV

Target ORF information:

RefSeq Version	XM_005270954.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005270954.2

ORF Insert Sequence:

ATGATCAGCT TTGTGGATCC TCTTGTGACC AATTACACAG TGGTGGACTT CAGAAATAGA 
GCAACTGCTC TGATTGAAGA TATATTTGCC CGAGACAAAA TTCCTATTGT TGTGGGAGGA 
ACCAATTATT ACATTGAATC TCTGCTCTGG AAAGTTCTTG TCAATACCAA GCCCCAGGAG 
ATGGGCACTG AGAAAGTGAT TGACCGAAAA GTGGAGCTTG AAAAGGAGGA TGGTCTTGTA 
CTTCACAAAC GCCTAAGCCA GGTGGACCCA GAAATGGCTG CCAAGCTGCA TCCACATGAC 
AAACGCAAAG TGGCCAGGAG CTTGCAAGTT TTTGAAGAAA CAGGAATCTC TCATAGTGAA 
TTTCTCCATC GTCAACATAC GGAAGAAGGT GGTGGTCCCC TTGGAGGTCC TCTGAAGTTC 
TCTAACCCTT GCATCCTTTG GCTTCATGCT GACCAGGCAG TTCTAGATGA GCGCTTGGAT 
AAGAGGGTGG ATGACATGCT TGCTGCTGGG CTCTTGGAGG AACTAAGAGA TTTTCACAGA 
CGCTATAATC AGAAGAATGT TTCGGAAAAT AGCCAGGACT ATCAACATGG TATCTTCCAA 
TCAATTGGCT TCAAGGAATT TCACGAGTAC CTGATCACTG AGGGAAAATG CACACTGGAG 
ACTAGTAACC AGCTTCTAAA GAAAGGTATT GAGGCTCTGA AACAAGTAAC TAAGAGATAT 
GCCCGGAAAC AAAACCGATG GGTTAAAAAC CGTTTTTTGA GCAGACCTGG TCCCATTGTC 
CCCCCTGTCT ATGGCTTAGA GGTATCTGAT GTCTCGAAGT GGGAAGAGTC TGTTCTTGAA 
CCTGCTCTTG AAATCGTGCA AAGTTTCATC CAGGGCCACA AGCCTACAGC CACTCCAATA 
AAGATGCCAT ACAATGAAGC TGAGAACAAG AGAAGTTATC ACCTGTGTGA CCTCTGTGAT 
CGAATCATCA TTGGGGATCG CGAATGGGCA GCGCACATAA AATCCAAATC CCACTTGAAC 
CAACTGAAGA AAAGAAGAAG ATTGGACTCA GATGCTGTCA ACACCATAGA AAGTCAGAGT 
GTTTCCCCAG ACCATAACAA AGAACCTAAA GAGAAGGGAT CCCCAGGGCA GAATGATCAA 
GAGCTGAAAT GCAGCGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIT1 ( XM_006710706.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006710769.1 Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu33527
Clone ID Related Accession (Same CDS sequence)	XM_006710706.1
Accession Version	XM_006710706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tRNA dimethylallyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACTG AGAAAGTGAT TGACCGAAAA GTGGAGCTTG AAAAGGAGGA TGGTCTTGTA 
CTTCACAAAC GCCTAAGCCA GGTGGACCCA GAAATGGCTG CCAAGCTGCA TCCACATGAC 
AAACGCAAAG TGGCCAGGAG CTTGCAAGTT TTTGAAGAAA CAGGAATCTC TCATAGTGAA 
TTTCTCCATC GTCAACATAC GGAAGAAGGT GGTGGTCCCC TTGGAGGTCC TCTGAAGTTC 
TCTAACCCTT GCATCCTTTG GCTTCATGCT GACCAGGCAG TTCTAGATGA GCGCTTGGAT 
AAGAGGGTGG ATGACATGCT TGCTGCTGGG CTCTTGGAGG AACTAAGAGA TTTTCACAGA 
CGCTATAATC AGAAGAATGT TTCGGAAAAT AGCCAGGACT ATCAACATGG TATCTTCCAA 
TCAATTGGCT TCAAGGAATT TCACGAGTAC CTGATCACTG AGGGAAAATG CACACTGGAG 
ACTAGTAACC AGCTTCTAAA GAAAGGTATT GAGGCTCTGA AACAAGTAAC TAAGAGATAT 
GCCCGGAAAC AAAACCGATG GGTTAAAAAC CGTTTTTTGA GCAGACCTGG TCCCATTGTC 
CCCCCTGTCT ATGGCTTAGA GGTATCTGAT GTCTCGAAGT GGGAAGAGTC TGTTCTTGAA 
CCTGCTCTTG AAATCGTGCA AAGTTTCATC CAGGGCCACA AGCCTACAGC CACTCCAATA 
AAGATGCCAT ACAATGAAGC TGAGAACAAG AGAAGTTATC ACCTGTGTGA CCTCTGTGAT 
CGAATCATCA TTGGGGATCG CGAATGGGCA GCGCACATAA AATCCAAATC CCACTTGAAC 
CAACTGAAGA AAAGAAGAAG ATTGGACTCA GATGCTGTCA ACACCATAGA AAGTCAGAGT 
GTTTCCCCAG ACCATAACAA AGAACCTAAA GAGAAGGGAT CCCCAGGGCA GAATGATCAA 
GAGCTGAAAT GCAGCGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006710769.1
CDS	512..1492
Translation	MGTEKVIDRKVELEKEDGLVLHKRLSQVDPEMAAKLHPHDKRKVARSLQVFEETGISHSEFLHRQHTEEGGGPLGGPLKFSNPCILWLHADQAVLDERLDKRVDDMLAAGLLEELRDFHRRYNQKNVSENSQDYQHGIFQSIGFKEFHEYLITEGKCTLETSNQLLKKGIEALKQVTKRYARKQNRWVKNRFLSRPGPIVPPVYGLEVSDVSKWEESVLEPALEIVQSFIQGHKPTATPIKMPYNEAENKRSYHLCDLCDRIIIGDREWAAHIKSKSHLNQLKKRRRLDSDAVNTIESQSVSPDHNKEPKEKGSPGQNDQELKCSV

Target ORF information:

RefSeq Version	XM_006710706.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA isopentenyltransferase 1 (TRIT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006710706.1

ORF Insert Sequence:

ATGGGCACTG AGAAAGTGAT TGACCGAAAA GTGGAGCTTG AAAAGGAGGA TGGTCTTGTA 
CTTCACAAAC GCCTAAGCCA GGTGGACCCA GAAATGGCTG CCAAGCTGCA TCCACATGAC 
AAACGCAAAG TGGCCAGGAG CTTGCAAGTT TTTGAAGAAA CAGGAATCTC TCATAGTGAA 
TTTCTCCATC GTCAACATAC GGAAGAAGGT GGTGGTCCCC TTGGAGGTCC TCTGAAGTTC 
TCTAACCCTT GCATCCTTTG GCTTCATGCT GACCAGGCAG TTCTAGATGA GCGCTTGGAT 
AAGAGGGTGG ATGACATGCT TGCTGCTGGG CTCTTGGAGG AACTAAGAGA TTTTCACAGA 
CGCTATAATC AGAAGAATGT TTCGGAAAAT AGCCAGGACT ATCAACATGG TATCTTCCAA 
TCAATTGGCT TCAAGGAATT TCACGAGTAC CTGATCACTG AGGGAAAATG CACACTGGAG 
ACTAGTAACC AGCTTCTAAA GAAAGGTATT GAGGCTCTGA AACAAGTAAC TAAGAGATAT 
GCCCGGAAAC AAAACCGATG GGTTAAAAAC CGTTTTTTGA GCAGACCTGG TCCCATTGTC 
CCCCCTGTCT ATGGCTTAGA GGTATCTGAT GTCTCGAAGT GGGAAGAGTC TGTTCTTGAA 
CCTGCTCTTG AAATCGTGCA AAGTTTCATC CAGGGCCACA AGCCTACAGC CACTCCAATA 
AAGATGCCAT ACAATGAAGC TGAGAACAAG AGAAGTTATC ACCTGTGTGA CCTCTGTGAT 
CGAATCATCA TTGGGGATCG CGAATGGGCA GCGCACATAA AATCCAAATC CCACTTGAAC 
CAACTGAAGA AAAGAAGAAG ATTGGACTCA GATGCTGTCA ACACCATAGA AAGTCAGAGT 
GTTTCCCCAG ACCATAACAA AGAACCTAAA GAGAAGGGAT CCCCAGGGCA GAATGATCAA 
GAGCTGAAAT GCAGCGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Matchmaking facilitates the diagnosis of an autosomal-recessive mitochondrial disease caused by biallelic mutation of the tRNA isopentenyltransferase (TRIT1) gene.
Human mutation38(5)511-516(2017 May)
Kernohan KD,Dyment DA,Pupavac M,Cramer Z,McBride A,Bernard G,Straub I,Tetreault M,Hartley T,Huang L,Sell E,Majewski J,Rosenblatt DS,Shoubridge E,Mhanni A,Myers T,Proud V,Vergano S,Spangler B,Farrow E,Kussman J,Safina N,,Saunders C,Boycott KM,Thiffault I

The human tRNA-modifying protein, TRIT1, forms amyloid fibers in vitro.
Gene61219-24(2017 May)
Waller TJ,Read DF,Engelke DR,Smaldino PJ

The cytoplasmic and nuclear populations of the eukaryote tRNA-isopentenyl transferase have distinct functions with implications in human cancer.
Gene556(1)13-8(2015 Feb)
Smaldino PJ,Read DF,Pratt-Hyatt M,Hopper AK,Engelke DR

Defective i6A37 modification of mitochondrial and cytosolic tRNAs results from pathogenic mutations in TRIT1 and its substrate tRNA.
PLoS genetics10(6)e1004424(2014 Jun)
Yarham JW,Lamichhane TN,Pyle A,Mattijssen S,Baruffini E,Bruni F,Donnini C,Vassilev A,He L,Blakely EL,Griffin H,Santibanez-Koref M,Bindoff LA,Ferrero I,Chinnery PF,McFarland R,Maraia RJ,Taylor RW

Human cells have a limited set of tRNA anticodon loop substrates of the tRNA isopentenyltransferase TRIT1 tumor suppressor.
Molecular and cellular biology33(24)4900-8(2013 Dec)
Lamichhane TN,Mattijssen S,Maraia RJ

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TRIT1 cDNA ORF clone, Homo sapiens(human)

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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