PPEF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPEF1
Entrez Gene ID	5475
Full Name	protein phosphatase with EF-hand domain 1
Synonyms	PP7,PPEF,PPP7C,PPP7CA
General protein information	Preferred Names protein phosphatase with EF-hand domain 1 Names serine/threonine-protein phosphatase with EF-hands 1 protein phosphatase 7, catalytic subunit, alpha isozyme protein phosphatase with EF calcium-binding domain protein phosphatase, EF-hand calcium binding domain 1 protein phosphatase, serine/threonine type, with EF-hands serine/threonine protein phosphatase 7
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp22.13
Summary	This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described. [provided by RefSeq, Jul 2008].
Disorder MIM:	300109

mRNA and Protein(s)

mRNA	Protein	Name
NM_006240.2	NP_006231.2	serine/threonine-protein phosphatase with EF-hands 1 isoform 1
NM_006240.3	NP_006231.2	serine/threonine-protein phosphatase with EF-hands 1 isoform 1
XM_005274553.2	XP_005274610.1	serine/threonine-protein phosphatase with EF-hands 1 isoform X1
XM_017029612.1	XP_016885101.1	serine/threonine-protein phosphatase with EF-hands 1 isoform X1
NM_152224.1	NP_689410.1	serine/threonine-protein phosphatase with EF-hands 1 isoform 1b
NM_152226.1	NP_689412.1	serine/threonine-protein phosphatase with EF-hands 1 isoform 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PPEF1	XP_003953993.1
Caenorhabditis elegans (roundworm)	pef-1	NP_001254836.1
	PPEF1	XP_001083653.1
Bos taurus (cattle)	PPEF1	XP_005228463.1
Gallus gallus (chicken)	PPEF1	XP_003640538.2
Canis lupus familiaris (dog)	PPEF1	XP_003640299.2
Homo sapiens (human)	PPEF1	NP_006231.2
Danio rerio (zebrafish)	ppef1	NP_001108202.1
Mus musculus (house mouse)	Ppef1	NP_035277.1
Rattus norvegicus (Norway rat)	Ppef1	NP_001030107.1
Xenopus tropicalis (tropical clawed frog)	ppef1	XP_002936093.2

Gene Ontology
Bibliography

Related articles in PubMed

Protein serine/threonine phosphatase PPEF-1 suppresses genotoxic stress response via dephosphorylation of PDCD5.
Park SY, Seo J, Choi HK, Oh HJ, Guk G, Lee YH, Lee J, Jun WJ, Choi KC, Yoon HG
Scientific reports739222(2017 Jan)

Molecular cloning of a novel PPEF-1 gene variant from a T-cell lymphoblastic lymphoma cell line.
Ho P, Dai KS, Chen HL
Recent patents on DNA & gene sequences6(1)72-7(2012 Apr)

The DNA sequence of the human X chromosome.
Ross MT, Grafham DV, Coffey AJ, Scherer S, McLay K, Muzny D, Platzer M, Howell GR, Burrows C, Bird CP, Frankish A, Lovell FL, Howe KL, Ashurst JL, Fulton RS, Sudbrak R, Wen G, Jones MC, Hurles ME, Andrews TD, Scott CE, Searle S, Ramser J, Whittaker A, Deadman R, Carter NP, Hunt SE, Chen R, Cree A, Gunaratne P, Havlak P, Hodgson A, Metzker ML, Richards S, Scott G, Steffen D, Sodergren E, Wheeler DA, Worley KC, Ainscough R, Ambrose KD, Ansari-Lari MA, Aradhya S, Ashwell RI, Babbage AK, Bagguley CL, Ballabio A, Banerjee R, Barker GE, Barlow KF, Barrett IP, Bates KN, Beare DM, Beasley H, Beasley O, Beck A, Bethel G, Blechschmidt K, Brady N, Bray-Allen S, Bridgeman AM, Brown AJ, Brown MJ, Bonnin D, Bruford EA, Buhay C, Burch P, Burford D, Burgess J, Burrill W, Burton J, Bye JM, Carder C, Carrel L, Chako J, Chapman JC, Chavez D, Chen E, Chen G, Chen Y, Chen Z, Chinault C, Ciccodicola A, Clark SY, Clarke G, Clee CM, Clegg S, Clerc-Blankenburg K, Clifford K, Cobley V, Cole CG, Conquer JS, Corby N, Connor RE, David R, Davies J, Davis C, Davis J, Delgado O, Deshazo D, Dhami P, Ding Y, Dinh H, Dodsworth S, Draper H, Dugan-Rocha S, Dunham A, Dunn M, Durbin KJ, Dutta I, Eades T, Ellwood M, Emery-Cohen A, Errington H, Evans KL, Faulkner L, Francis F, Frankland J, Fraser AE, Galgoczy P, Gilbert J, Gill R, Gl?ckner G, Gregory SG, Gribble S, Griffiths C, Grocock R, Gu Y, Gwilliam R, Hamilton C, Hart EA, Hawes A, Heath PD, Heitmann K, Hennig S, Hernandez J, Hinzmann B, Ho S, Hoffs M, Howden PJ, Huckle EJ, Hume J, Hunt PJ, Hunt AR, Isherwood J, Jacob L, Johnson D, Jones S, de Jong PJ, Joseph SS, Keenan S, Kelly S, Kershaw JK, Khan Z, Kioschis P, Klages S, Knights AJ, Kosiura A, Kovar-Smith C, Laird GK, Langford C, Lawlor S, Leversha M, Lewis L, Liu W, Lloyd C, Lloyd DM, Loulseged H, Loveland JE, Lovell JD, Lozado R, Lu J, Lyne R, Ma J, Maheshwari M, Matthews LH, McDowall J, McLaren S, McMurray A, Meidl P, Meitinger T, Milne S, Miner G, Mistry SL, Morgan M, Morris S, M?ller I, Mullikin JC, Nguyen N, Nordsiek G, Nyakatura G, O'Dell CN, Okwuonu G, Palmer S, Pandian R, Parker D, Parrish J, Pasternak S, Patel D, Pearce AV, Pearson DM, Pelan SE, Perez L, Porter KM, Ramsey Y, Reichwald K, Rhodes S, Ridler KA, Schlessinger D, Schueler MG, Sehra HK, Shaw-Smith C, Shen H, Sheridan EM, Shownkeen R, Skuce CD, Smith ML, Sotheran EC, Steingruber HE, Steward CA, Storey R, Swann RM, Swarbreck D, Tabor PE, Taudien S, Taylor T, Teague B, Thomas K, Thorpe A, Timms K, Tracey A, Trevanion S, Tromans AC, d'Urso M, Verduzco D, Villasana D, Waldron L, Wall M, Wang Q, Warren J, Warry GL, Wei X, West A, Whitehead SL, Whiteley MN, Wilkinson JE, Willey DL, Williams G, Williams L, Williamson A, Williamson H, Wilming L, Woodmansey RL, Wray PW, Yen J, Zhang J, Zhou J, Zoghbi H, Zorilla S, Buck D, Reinhardt R, Poustka A, Rosenthal A, Lehrach H, Meindl A, Minx PJ, Hillier LW, Willard HF, Wilson RK, Waterston RH, Rice CM, Vaudin M, Coulson A, Nelson DL, Weinstock G, Sulston JE, Durbin R, Hubbard T, Gibbs RA, Beck S, Rogers J, Bentley DR
Nature434(7031)325-37(2005 Mar)

An unappreciated role for RNA surveillance.
Hillman RT, Green RE, Brenner SE
Genome biology5(2)R8(2004)

Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.
Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J
Nature biotechnology21(5)566-9(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PPEF-1V is a 350bp deletion in the nucleotide sequence of PPEF-1 from 128-477bp and a 152-amino-acid N-terminal deletion in the amino acid sequence of PPEF-1.
Title: Molecular cloning of a novel PPEF-1 gene variant from a T-cell lymphoblastic lymphoma cell line.

The following PPEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06686	NM_152224.1 Latest version!	Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant 1b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu18253	NM_006240.3 Latest version!	Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu18253	XM_005274553.2 Latest version!	Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu53937	XM_017029612.1 Latest version!	Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu06687	NM_152226.1 Latest version!	Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu18253	NM_006240.2	Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPEF1 ( NM_152224.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_689410.1 Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant 1b, mRNA.

Price & Availability↑

CloneID	OHu06686
Clone ID Related Accession (Same CDS sequence)	NM_152224.1
Accession Version	NM_152224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase with EF-hands 1 isoform 1b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X97867.1. Transcript Variant: This variant (1b) uses an alternate in-frame splice site compared to variant 1, resulting in an isoform (1b) that lacks an internal segment compared to isoform 1. Isoform 1b contains a catalytic domain and two EF-hand motifs. Its functional significance is unknown. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X97867.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2148093 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGATGCA GCAGTTCTTC AACGAAAACC AGGAGATCTG ACACATCACT GAGAGCTGCG 
TTGATCATCC AGAACTGGTA CCGAGGTTAC AAAGCTCGAC TGAAGGCCAG ACAACACTAT 
GCCCTCACCA TCTTCCAGTC CATCGAATAT GCTGATGAAC AAGGCCAAAT GCAGTTATCC 
ACCTTCTTTT CCTTCATGTT GGAAAACTAC ACACATATAC ATAAGGAAGA GCTAGAATTA 
AGAAATCAGT CTCTTGAAAG CGAACAGGAC ATGAGGGATA GATGGGATTA TGTGGACTCG 
ATAGATGTCC CAGACTCCTA TAATGGTCCT CGGCTACAAT TTCCTCTCAC TTGTACGGAT 
ATTGATTTAC TTCTTGAGGC CTTCAAGGAA CAACAGATAC TTCATGCCCA TTATGTCTTA 
GAGGTGCTAT TTGAAACCAA GAAAGTCCTG AAGCAAATGC CGAATTTCAC TCACATACAA 
ACTTCTCCCT CCAAAGAGGT AACAATCTGT GGTGATTTGC ATGGGAAACT GGATGATCTT 
TTTTTGATCT TCTACAAGAA TGGTCTCCCC TCAGAGAGGA ACCCGTATGT TTTTAATGGT 
GACTTTGTAG ATCGAGGAAA GAATTCCATA GAGATCCTAA TGATCCTGTG TGTGAGTTTT 
CTTGTCTACC CCAATGACCT GCACTTGAAC AGAGGGAACC ACGAAGATTT TATGATGAAT 
CTGAGGTATG GCTTCACGAA AGAAATTTTG CATAAATATA AGCTACATGG AAAAAGAATC 
TTACAAATCT TGGAAGAATT CTATGCCTGG CTCCCAATCG GTACAATCGT TGACAATGAA 
ATCCTGGTCA TCCATGGTGG GATATCAGAG ACCACAGACT TGAATTTACT CCACCGTGTA 
GAGAGGAACA AGATGAAATC TGTGCTGATA CCACCAACGG AAACAAACAG AGACCATGAC 
ACTGACTCGA AGCACAATAA AATTATTGAT ATTCTGTGGA GTGATCCCAG AGGCAAAAAT 
GGCTGTTTTC CAAATACGTG CCGAGGAGGG GGCTGCTATT TTGGACCAGA TGTTACTTCC 
AAGATTCTTA ATAAATACCA GTTGAAGATG CTCATCAGGT CTCATGAATG TAAGCCCGAA 
GGGTATGAAA TCTGTCATGA TGGGAAGGTG GTGACTATAT TTTCTGCTTC TAATTATTAT 
GAAGAAGGCA GCAATCGAGG AGCTTACATC AAACTATGTT CTGGTACAAC TCCTCGATTT 
TTCCAGTACC AAGTAACTAA AGCAACGTGC TTTCAGCCTC TTCGCCAAAG AGTGGATACT 
ATGGAAAACA GCGCCATCAA GATATTAAGA GAGAGAGTGA TTTCACGAAA AAGTGACCTT 
ACTCGTGCTT TCCAACTTCA AGACCACAGA AAATCAGGAA AACTTTCTGT GAGCCAGTGG 
GCTTTTTGCA TGGAGAACAT TTTGGGGCTG AACTTACCAT GGAGATCCCT CAGTTCGAAT 
CTGGTAAACA TAGACCAAAA TGGAAACGTT GAATACATGT CCAGCTTCCA GAATATCCGC 
ATTGAAAAAC CTGTACAAGA GGCTCATTCT ACTCTAGTTG AAACTCTGTA CAGATACAGA 
TCTGACCTGG AAATCATATT TAATGCCATT GACACTGATC ACTCAGGCCT GATCTCCGTG 
GAAGAATTTC GTGCCATGTG GAAACTTTTT AGTTCTCACT ACAATGTTCA CATTGATGAT 
TCCCAAGTCA ATAAGCTTGC CAACATAATG GACTTGAACA AAGATGGAAG CATTGACTTT 
AATGAGTTTT TAAAGGCTTT CTATGTAGTG CATAGATATG AAGACTTGAT GAAACCTGAT 
GTCACCAACC TTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689410.1
CDS	482..2359
Translation	MGCSSSSTKTRRSDTSLRAALIIQNWYRGYKARLKARQHYALTIFQSIEYADEQGQMQLSTFFSFMLENYTHIHKEELELRNQSLESEQDMRDRWDYVDSIDVPDSYNGPRLQFPLTCTDIDLLLEAFKEQQILHAHYVLEVLFETKKVLKQMPNFTHIQTSPSKEVTICGDLHGKLDDLFLIFYKNGLPSERNPYVFNGDFVDRGKNSIEILMILCVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKLHGKRILQILEEFYAWLPIGTIVDNEILVIHGGISETTDLNLLHRVERNKMKSVLIPPTETNRDHDTDSKHNKIIDILWSDPRGKNGCFPNTCRGGGCYFGPDVTSKILNKYQLKMLIRSHECKPEGYEICHDGKVVTIFSASNYYEEGSNRGAYIKLCSGTTPRFFQYQVTKATCFQPLRQRVDTMENSAIKILRERVISRKSDLTRAFQLQDHRKSGKLSVSQWAFCMENILGLNLPWRSLSSNLVNIDQNGNVEYMSSFQNIRIEKPVQEAHSTLVETLYRYRSDLEIIFNAIDTDHSGLISVEEFRAMWKLFSSHYNVHIDDSQVNKLANIMDLNKDGSIDFNEFLKAFYVVHRYEDLMKPDVTNLG

Target ORF information:

RefSeq Version	NM_152224.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant 1b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152224.1

ORF Insert Sequence:

ATGGGATGCA GCAGTTCTTC AACGAAAACC AGGAGATCTG ACACATCACT GAGAGCTGCG 
TTGATCATCC AGAACTGGTA CCGAGGTTAC AAAGCTCGAC TGAAGGCCAG ACAACACTAT 
GCCCTCACCA TCTTCCAGTC CATCGAATAT GCTGATGAAC AAGGCCAAAT GCAGTTATCC 
ACCTTCTTTT CCTTCATGTT GGAAAACTAC ACACATATAC ATAAGGAAGA GCTAGAATTA 
AGAAATCAGT CTCTTGAAAG CGAACAGGAC ATGAGGGATA GATGGGATTA TGTGGACTCG 
ATAGATGTCC CAGACTCCTA TAATGGTCCT CGGCTACAAT TTCCTCTCAC TTGTACGGAT 
ATTGATTTAC TTCTTGAGGC CTTCAAGGAA CAACAGATAC TTCATGCCCA TTATGTCTTA 
GAGGTGCTAT TTGAAACCAA GAAAGTCCTG AAGCAAATGC CGAATTTCAC TCACATACAA 
ACTTCTCCCT CCAAAGAGGT AACAATCTGT GGTGATTTGC ATGGGAAACT GGATGATCTT 
TTTTTGATCT TCTACAAGAA TGGTCTCCCC TCAGAGAGGA ACCCGTATGT TTTTAATGGT 
GACTTTGTAG ATCGAGGAAA GAATTCCATA GAGATCCTAA TGATCCTGTG TGTGAGTTTT 
CTTGTCTACC CCAATGACCT GCACTTGAAC AGAGGGAACC ACGAAGATTT TATGATGAAT 
CTGAGGTATG GCTTCACGAA AGAAATTTTG CATAAATATA AGCTACATGG AAAAAGAATC 
TTACAAATCT TGGAAGAATT CTATGCCTGG CTCCCAATCG GTACAATCGT TGACAATGAA 
ATCCTGGTCA TCCATGGTGG GATATCAGAG ACCACAGACT TGAATTTACT CCACCGTGTA 
GAGAGGAACA AGATGAAATC TGTGCTGATA CCACCAACGG AAACAAACAG AGACCATGAC 
ACTGACTCGA AGCACAATAA AATTATTGAT ATTCTGTGGA GTGATCCCAG AGGCAAAAAT 
GGCTGTTTTC CAAATACGTG CCGAGGAGGG GGCTGCTATT TTGGACCAGA TGTTACTTCC 
AAGATTCTTA ATAAATACCA GTTGAAGATG CTCATCAGGT CTCATGAATG TAAGCCCGAA 
GGGTATGAAA TCTGTCATGA TGGGAAGGTG GTGACTATAT TTTCTGCTTC TAATTATTAT 
GAAGAAGGCA GCAATCGAGG AGCTTACATC AAACTATGTT CTGGTACAAC TCCTCGATTT 
TTCCAGTACC AAGTAACTAA AGCAACGTGC TTTCAGCCTC TTCGCCAAAG AGTGGATACT 
ATGGAAAACA GCGCCATCAA GATATTAAGA GAGAGAGTGA TTTCACGAAA AAGTGACCTT 
ACTCGTGCTT TCCAACTTCA AGACCACAGA AAATCAGGAA AACTTTCTGT GAGCCAGTGG 
GCTTTTTGCA TGGAGAACAT TTTGGGGCTG AACTTACCAT GGAGATCCCT CAGTTCGAAT 
CTGGTAAACA TAGACCAAAA TGGAAACGTT GAATACATGT CCAGCTTCCA GAATATCCGC 
ATTGAAAAAC CTGTACAAGA GGCTCATTCT ACTCTAGTTG AAACTCTGTA CAGATACAGA 
TCTGACCTGG AAATCATATT TAATGCCATT GACACTGATC ACTCAGGCCT GATCTCCGTG 
GAAGAATTTC GTGCCATGTG GAAACTTTTT AGTTCTCACT ACAATGTTCA CATTGATGAT 
TCCCAAGTCA ATAAGCTTGC CAACATAATG GACTTGAACA AAGATGGAAG CATTGACTTT 
AATGAGTTTT TAAAGGCTTT CTATGTAGTG CATAGATATG AAGACTTGAT GAAACCTGAT 
GTCACCAACC TTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( NM_006240.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006231.2 Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18253
Clone ID Related Accession (Same CDS sequence)	NM_006240.2 , NM_006240.3 , XM_005274553.2
Accession Version	NM_006240.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase with EF-hands 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X97867.1. This sequence is a reference standard in the RefSeqGene project. On Sep 25, 2002 this sequence version replaced NM_006240.1. Transcript Variant: This variant (1) encodes the predominant isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036026.1, X97867.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGATGCA GCAGTTCTTC AACGAAAACC AGGAGATCTG ACACATCACT GAGAGCTGCG 
TTGATCATCC AGAACTGGTA CCGAGGTTAC AAAGCTCGAC TGAAGGCCAG ACAACACTAT 
GCCCTCACCA TCTTCCAGTC CATCGAATAT GCTGATGAAC AAGGCCAAAT GCAGTTATCC 
ACCTTCTTTT CCTTCATGTT GGAAAACTAC ACACATATAC ATAAGGAAGA GCTAGAATTA 
AGAAATCAGT CTCTTGAAAG CGAACAGGAC ATGAGGGATA GATGGGATTA TGTGGACTCG 
ATAGATGTCC CAGACTCCTA TAATGGTCCT CGGCTACAAT TTCCTCTCAC TTGTACGGAT 
ATTGATTTAC TTCTTGAGGC CTTCAAGGAA CAACAGATAC TTCATGCCCA TTATGTCTTA 
GAGGTGCTAT TTGAAACCAA GAAAGTCCTG AAGCAAATGC CGAATTTCAC TCACATACAA 
ACTTCTCCCT CCAAAGAGGT AACAATCTGT GGTGATTTGC ATGGGAAACT GGATGATCTT 
TTTTTGATCT TCTACAAGAA TGGTCTCCCC TCAGAGAGGA ACCCGTATGT TTTTAATGGT 
GACTTTGTAG ATCGAGGAAA GAATTCCATA GAGATCCTAA TGATCCTGTG TGTGAGTTTT 
CTTGTCTACC CCAATGACCT GCACTTGAAC AGAGGGAACC ACGAAGATTT TATGATGAAT 
CTGAGGTATG GCTTCACGAA AGAAATTTTG CATAAATATA AGCTACATGG AAAAAGAATC 
TTACAAATCT TGGAAGAATT CTATGCCTGG CTCCCAATCG GTACAATCGT TGACAATGAA 
ATCCTGGTCA TCCATGGTGG GATATCAGAG ACCACAGACT TGAATTTACT CCACCGTGTA 
GAGAGGAACA AGATGAAATC TGTGCTGATA CCACCAACGG AAACAAACAG AGACCATGAC 
ACTGACTCGA AGCACAATAA AGTAGGTGTG ACTTTTAATG CACATGGAAG AATCAAAACA 
AATGGATCTC CTACTGAACA CTTAACAGAG CATGAATGGG AACAGATTAT TGATATTCTG 
TGGAGTGATC CCAGAGGCAA AAATGGCTGT TTTCCAAATA CGTGCCGAGG AGGGGGCTGC 
TATTTTGGAC CAGATGTTAC TTCCAAGATT CTTAATAAAT ACCAGTTGAA GATGCTCATC 
AGGTCTCATG AATGTAAGCC CGAAGGGTAT GAAATCTGTC ATGATGGGAA GGTGGTGACT 
ATATTTTCTG CTTCTAATTA TTATGAAGAA GGCAGCAATC GAGGAGCTTA CATCAAACTA 
TGTTCTGGTA CAACTCCTCG ATTTTTCCAG TACCAAGTAA CTAAAGCAAC GTGCTTTCAG 
CCTCTTCGCC AAAGAGTGGA TACTATGGAA AACAGCGCCA TCAAGATATT AAGAGAGAGA 
GTGATTTCAC GAAAAAGTGA CCTTACTCGT GCTTTCCAAC TTCAAGACCA CAGAAAATCA 
GGAAAACTTT CTGTGAGCCA GTGGGCTTTT TGCATGGAGA ACATTTTGGG GCTGAACTTA 
CCATGGAGAT CCCTCAGTTC GAATCTGGTA AACATAGACC AAAATGGAAA CGTTGAATAC 
ATGTCCAGCT TCCAGAATAT CCGCATTGAA AAACCTGTAC AAGAGGCTCA TTCTACTCTA 
GTTGAAACTC TGTACAGATA CAGATCTGAC CTGGAAATCA TATTTAATGC CATTGACACT 
GATCACTCAG GCCTGATCTC CGTGGAAGAA TTTCGTGCCA TGTGGAAACT TTTTAGTTCT 
CACTACAATG TTCACATTGA TGATTCCCAA GTCAATAAGC TTGCCAACAT AATGGACTTG 
AACAAAGATG GAAGCATTGA CTTTAATGAG TTTTTAAAGG CTTTCTATGT AGTGCATAGA 
TATGAAGACT TGATGAAACC TGATGTCACC AACCTTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006231.2
CDS	482..2443
Translation	MGCSSSSTKTRRSDTSLRAALIIQNWYRGYKARLKARQHYALTIFQSIEYADEQGQMQLSTFFSFMLENYTHIHKEELELRNQSLESEQDMRDRWDYVDSIDVPDSYNGPRLQFPLTCTDIDLLLEAFKEQQILHAHYVLEVLFETKKVLKQMPNFTHIQTSPSKEVTICGDLHGKLDDLFLIFYKNGLPSERNPYVFNGDFVDRGKNSIEILMILCVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKLHGKRILQILEEFYAWLPIGTIVDNEILVIHGGISETTDLNLLHRVERNKMKSVLIPPTETNRDHDTDSKHNKVGVTFNAHGRIKTNGSPTEHLTEHEWEQIIDILWSDPRGKNGCFPNTCRGGGCYFGPDVTSKILNKYQLKMLIRSHECKPEGYEICHDGKVVTIFSASNYYEEGSNRGAYIKLCSGTTPRFFQYQVTKATCFQPLRQRVDTMENSAIKILRERVISRKSDLTRAFQLQDHRKSGKLSVSQWAFCMENILGLNLPWRSLSSNLVNIDQNGNVEYMSSFQNIRIEKPVQEAHSTLVETLYRYRSDLEIIFNAIDTDHSGLISVEEFRAMWKLFSSHYNVHIDDSQVNKLANIMDLNKDGSIDFNEFLKAFYVVHRYEDLMKPDVTNLG

Target ORF information:

RefSeq Version	NM_006240.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006240.2

ORF Insert Sequence:

ATGGGATGCA GCAGTTCTTC AACGAAAACC AGGAGATCTG ACACATCACT GAGAGCTGCG 
TTGATCATCC AGAACTGGTA CCGAGGTTAC AAAGCTCGAC TGAAGGCCAG ACAACACTAT 
GCCCTCACCA TCTTCCAGTC CATCGAATAT GCTGATGAAC AAGGCCAAAT GCAGTTATCC 
ACCTTCTTTT CCTTCATGTT GGAAAACTAC ACACATATAC ATAAGGAAGA GCTAGAATTA 
AGAAATCAGT CTCTTGAAAG CGAACAGGAC ATGAGGGATA GATGGGATTA TGTGGACTCG 
ATAGATGTCC CAGACTCCTA TAATGGTCCT CGGCTACAAT TTCCTCTCAC TTGTACGGAT 
ATTGATTTAC TTCTTGAGGC CTTCAAGGAA CAACAGATAC TTCATGCCCA TTATGTCTTA 
GAGGTGCTAT TTGAAACCAA GAAAGTCCTG AAGCAAATGC CGAATTTCAC TCACATACAA 
ACTTCTCCCT CCAAAGAGGT AACAATCTGT GGTGATTTGC ATGGGAAACT GGATGATCTT 
TTTTTGATCT TCTACAAGAA TGGTCTCCCC TCAGAGAGGA ACCCGTATGT TTTTAATGGT 
GACTTTGTAG ATCGAGGAAA GAATTCCATA GAGATCCTAA TGATCCTGTG TGTGAGTTTT 
CTTGTCTACC CCAATGACCT GCACTTGAAC AGAGGGAACC ACGAAGATTT TATGATGAAT 
CTGAGGTATG GCTTCACGAA AGAAATTTTG CATAAATATA AGCTACATGG AAAAAGAATC 
TTACAAATCT TGGAAGAATT CTATGCCTGG CTCCCAATCG GTACAATCGT TGACAATGAA 
ATCCTGGTCA TCCATGGTGG GATATCAGAG ACCACAGACT TGAATTTACT CCACCGTGTA 
GAGAGGAACA AGATGAAATC TGTGCTGATA CCACCAACGG AAACAAACAG AGACCATGAC 
ACTGACTCGA AGCACAATAA AGTAGGTGTG ACTTTTAATG CACATGGAAG AATCAAAACA 
AATGGATCTC CTACTGAACA CTTAACAGAG CATGAATGGG AACAGATTAT TGATATTCTG 
TGGAGTGATC CCAGAGGCAA AAATGGCTGT TTTCCAAATA CGTGCCGAGG AGGGGGCTGC 
TATTTTGGAC CAGATGTTAC TTCCAAGATT CTTAATAAAT ACCAGTTGAA GATGCTCATC 
AGGTCTCATG AATGTAAGCC CGAAGGGTAT GAAATCTGTC ATGATGGGAA GGTGGTGACT 
ATATTTTCTG CTTCTAATTA TTATGAAGAA GGCAGCAATC GAGGAGCTTA CATCAAACTA 
TGTTCTGGTA CAACTCCTCG ATTTTTCCAG TACCAAGTAA CTAAAGCAAC GTGCTTTCAG 
CCTCTTCGCC AAAGAGTGGA TACTATGGAA AACAGCGCCA TCAAGATATT AAGAGAGAGA 
GTGATTTCAC GAAAAAGTGA CCTTACTCGT GCTTTCCAAC TTCAAGACCA CAGAAAATCA 
GGAAAACTTT CTGTGAGCCA GTGGGCTTTT TGCATGGAGA ACATTTTGGG GCTGAACTTA 
CCATGGAGAT CCCTCAGTTC GAATCTGGTA AACATAGACC AAAATGGAAA CGTTGAATAC 
ATGTCCAGCT TCCAGAATAT CCGCATTGAA AAACCTGTAC AAGAGGCTCA TTCTACTCTA 
GTTGAAACTC TGTACAGATA CAGATCTGAC CTGGAAATCA TATTTAATGC CATTGACACT 
GATCACTCAG GCCTGATCTC CGTGGAAGAA TTTCGTGCCA TGTGGAAACT TTTTAGTTCT 
CACTACAATG TTCACATTGA TGATTCCCAA GTCAATAAGC TTGCCAACAT AATGGACTTG 
AACAAAGATG GAAGCATTGA CTTTAATGAG TTTTTAAAGG CTTTCTATGT AGTGCATAGA 
TATGAAGACT TGATGAAACC TGATGTCACC AACCTTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( NM_006240.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006231.2 Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant 1, mRNA.

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CloneID	OHu18253
Clone ID Related Accession (Same CDS sequence)	NM_006240.2 , NM_006240.3 , XM_005274553.2
Accession Version	NM_006240.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase with EF-hands 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z94056.1 and AL096700.14. On Dec 4, 2019 this sequence version replaced NM_006240.2. Transcript Variant: This variant (1) encodes the predominant isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036026.1, AK292235.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGATGCA GCAGTTCTTC AACGAAAACC AGGAGATCTG ACACATCACT GAGAGCTGCG 
TTGATCATCC AGAACTGGTA CCGAGGTTAC AAAGCTCGAC TGAAGGCCAG ACAACACTAT 
GCCCTCACCA TCTTCCAGTC CATCGAATAT GCTGATGAAC AAGGCCAAAT GCAGTTATCC 
ACCTTCTTTT CCTTCATGTT GGAAAACTAC ACACATATAC ATAAGGAAGA GCTAGAATTA 
AGAAATCAGT CTCTTGAAAG CGAACAGGAC ATGAGGGATA GATGGGATTA TGTGGACTCG 
ATAGATGTCC CAGACTCCTA TAATGGTCCT CGGCTACAAT TTCCTCTCAC TTGTACGGAT 
ATTGATTTAC TTCTTGAGGC CTTCAAGGAA CAACAGATAC TTCATGCCCA TTATGTCTTA 
GAGGTGCTAT TTGAAACCAA GAAAGTCCTG AAGCAAATGC CGAATTTCAC TCACATACAA 
ACTTCTCCCT CCAAAGAGGT AACAATCTGT GGTGATTTGC ATGGGAAACT GGATGATCTT 
TTTTTGATCT TCTACAAGAA TGGTCTCCCC TCAGAGAGGA ACCCGTATGT TTTTAATGGT 
GACTTTGTAG ATCGAGGAAA GAATTCCATA GAGATCCTAA TGATCCTGTG TGTGAGTTTT 
CTTGTCTACC CCAATGACCT GCACTTGAAC AGAGGGAACC ACGAAGATTT TATGATGAAT 
CTGAGGTATG GCTTCACGAA AGAAATTTTG CATAAATATA AGCTACATGG AAAAAGAATC 
TTACAAATCT TGGAAGAATT CTATGCCTGG CTCCCAATCG GTACAATCGT TGACAATGAA 
ATCCTGGTCA TCCATGGTGG GATATCAGAG ACCACAGACT TGAATTTACT CCACCGTGTA 
GAGAGGAACA AGATGAAATC TGTGCTGATA CCACCAACGG AAACAAACAG AGACCATGAC 
ACTGACTCGA AGCACAATAA AGTAGGTGTG ACTTTTAATG CACATGGAAG AATCAAAACA 
AATGGATCTC CTACTGAACA CTTAACAGAG CATGAATGGG AACAGATTAT TGATATTCTG 
TGGAGTGATC CCAGAGGCAA AAATGGCTGT TTTCCAAATA CGTGCCGAGG AGGGGGCTGC 
TATTTTGGAC CAGATGTTAC TTCCAAGATT CTTAATAAAT ACCAGTTGAA GATGCTCATC 
AGGTCTCATG AATGTAAGCC CGAAGGGTAT GAAATCTGTC ATGATGGGAA GGTGGTGACT 
ATATTTTCTG CTTCTAATTA TTATGAAGAA GGCAGCAATC GAGGAGCTTA CATCAAACTA 
TGTTCTGGTA CAACTCCTCG ATTTTTCCAG TACCAAGTAA CTAAAGCAAC GTGCTTTCAG 
CCTCTTCGCC AAAGAGTGGA TACTATGGAA AACAGCGCCA TCAAGATATT AAGAGAGAGA 
GTGATTTCAC GAAAAAGTGA CCTTACTCGT GCTTTCCAAC TTCAAGACCA CAGAAAATCA 
GGAAAACTTT CTGTGAGCCA GTGGGCTTTT TGCATGGAGA ACATTTTGGG GCTGAACTTA 
CCATGGAGAT CCCTCAGTTC GAATCTGGTA AACATAGACC AAAATGGAAA CGTTGAATAC 
ATGTCCAGCT TCCAGAATAT CCGCATTGAA AAACCTGTAC AAGAGGCTCA TTCTACTCTA 
GTTGAAACTC TGTACAGATA CAGATCTGAC CTGGAAATCA TATTTAATGC CATTGACACT 
GATCACTCAG GCCTGATCTC CGTGGAAGAA TTTCGTGCCA TGTGGAAACT TTTTAGTTCT 
CACTACAATG TTCACATTGA TGATTCCCAA GTCAATAAGC TTGCCAACAT AATGGACTTG 
AACAAAGATG GAAGCATTGA CTTTAATGAG TTTTTAAAGG CTTTCTATGT AGTGCATAGA 
TATGAAGACT TGATGAAACC TGATGTCACC AACCTTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006231.2
CDS	504..2465
Translation	MGCSSSSTKTRRSDTSLRAALIIQNWYRGYKARLKARQHYALTIFQSIEYADEQGQMQLSTFFSFMLENYTHIHKEELELRNQSLESEQDMRDRWDYVDSIDVPDSYNGPRLQFPLTCTDIDLLLEAFKEQQILHAHYVLEVLFETKKVLKQMPNFTHIQTSPSKEVTICGDLHGKLDDLFLIFYKNGLPSERNPYVFNGDFVDRGKNSIEILMILCVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKLHGKRILQILEEFYAWLPIGTIVDNEILVIHGGISETTDLNLLHRVERNKMKSVLIPPTETNRDHDTDSKHNKVGVTFNAHGRIKTNGSPTEHLTEHEWEQIIDILWSDPRGKNGCFPNTCRGGGCYFGPDVTSKILNKYQLKMLIRSHECKPEGYEICHDGKVVTIFSASNYYEEGSNRGAYIKLCSGTTPRFFQYQVTKATCFQPLRQRVDTMENSAIKILRERVISRKSDLTRAFQLQDHRKSGKLSVSQWAFCMENILGLNLPWRSLSSNLVNIDQNGNVEYMSSFQNIRIEKPVQEAHSTLVETLYRYRSDLEIIFNAIDTDHSGLISVEEFRAMWKLFSSHYNVHIDDSQVNKLANIMDLNKDGSIDFNEFLKAFYVVHRYEDLMKPDVTNLG

Target ORF information:

RefSeq Version	NM_006240.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006240.3

ORF Insert Sequence:

ATGGGATGCA GCAGTTCTTC AACGAAAACC AGGAGATCTG ACACATCACT GAGAGCTGCG 
TTGATCATCC AGAACTGGTA CCGAGGTTAC AAAGCTCGAC TGAAGGCCAG ACAACACTAT 
GCCCTCACCA TCTTCCAGTC CATCGAATAT GCTGATGAAC AAGGCCAAAT GCAGTTATCC 
ACCTTCTTTT CCTTCATGTT GGAAAACTAC ACACATATAC ATAAGGAAGA GCTAGAATTA 
AGAAATCAGT CTCTTGAAAG CGAACAGGAC ATGAGGGATA GATGGGATTA TGTGGACTCG 
ATAGATGTCC CAGACTCCTA TAATGGTCCT CGGCTACAAT TTCCTCTCAC TTGTACGGAT 
ATTGATTTAC TTCTTGAGGC CTTCAAGGAA CAACAGATAC TTCATGCCCA TTATGTCTTA 
GAGGTGCTAT TTGAAACCAA GAAAGTCCTG AAGCAAATGC CGAATTTCAC TCACATACAA 
ACTTCTCCCT CCAAAGAGGT AACAATCTGT GGTGATTTGC ATGGGAAACT GGATGATCTT 
TTTTTGATCT TCTACAAGAA TGGTCTCCCC TCAGAGAGGA ACCCGTATGT TTTTAATGGT 
GACTTTGTAG ATCGAGGAAA GAATTCCATA GAGATCCTAA TGATCCTGTG TGTGAGTTTT 
CTTGTCTACC CCAATGACCT GCACTTGAAC AGAGGGAACC ACGAAGATTT TATGATGAAT 
CTGAGGTATG GCTTCACGAA AGAAATTTTG CATAAATATA AGCTACATGG AAAAAGAATC 
TTACAAATCT TGGAAGAATT CTATGCCTGG CTCCCAATCG GTACAATCGT TGACAATGAA 
ATCCTGGTCA TCCATGGTGG GATATCAGAG ACCACAGACT TGAATTTACT CCACCGTGTA 
GAGAGGAACA AGATGAAATC TGTGCTGATA CCACCAACGG AAACAAACAG AGACCATGAC 
ACTGACTCGA AGCACAATAA AGTAGGTGTG ACTTTTAATG CACATGGAAG AATCAAAACA 
AATGGATCTC CTACTGAACA CTTAACAGAG CATGAATGGG AACAGATTAT TGATATTCTG 
TGGAGTGATC CCAGAGGCAA AAATGGCTGT TTTCCAAATA CGTGCCGAGG AGGGGGCTGC 
TATTTTGGAC CAGATGTTAC TTCCAAGATT CTTAATAAAT ACCAGTTGAA GATGCTCATC 
AGGTCTCATG AATGTAAGCC CGAAGGGTAT GAAATCTGTC ATGATGGGAA GGTGGTGACT 
ATATTTTCTG CTTCTAATTA TTATGAAGAA GGCAGCAATC GAGGAGCTTA CATCAAACTA 
TGTTCTGGTA CAACTCCTCG ATTTTTCCAG TACCAAGTAA CTAAAGCAAC GTGCTTTCAG 
CCTCTTCGCC AAAGAGTGGA TACTATGGAA AACAGCGCCA TCAAGATATT AAGAGAGAGA 
GTGATTTCAC GAAAAAGTGA CCTTACTCGT GCTTTCCAAC TTCAAGACCA CAGAAAATCA 
GGAAAACTTT CTGTGAGCCA GTGGGCTTTT TGCATGGAGA ACATTTTGGG GCTGAACTTA 
CCATGGAGAT CCCTCAGTTC GAATCTGGTA AACATAGACC AAAATGGAAA CGTTGAATAC 
ATGTCCAGCT TCCAGAATAT CCGCATTGAA AAACCTGTAC AAGAGGCTCA TTCTACTCTA 
GTTGAAACTC TGTACAGATA CAGATCTGAC CTGGAAATCA TATTTAATGC CATTGACACT 
GATCACTCAG GCCTGATCTC CGTGGAAGAA TTTCGTGCCA TGTGGAAACT TTTTAGTTCT 
CACTACAATG TTCACATTGA TGATTCCCAA GTCAATAAGC TTGCCAACAT AATGGACTTG 
AACAAAGATG GAAGCATTGA CTTTAATGAG TTTTTAAAGG CTTTCTATGT AGTGCATAGA 
TATGAAGACT TGATGAAACC TGATGTCACC AACCTTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( XM_005274553.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274610.1 Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X1, mRNA.

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CloneID	OHu18253
Clone ID Related Accession (Same CDS sequence)	NM_006240.2 , NM_006240.3 , XM_005274553.2
Accession Version	XM_005274553.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase with EF-hands 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005274553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA GCAGTTCTTC AACGAAAACC AGGAGATCTG ACACATCACT GAGAGCTGCG 
TTGATCATCC AGAACTGGTA CCGAGGTTAC AAAGCTCGAC TGAAGGCCAG ACAACACTAT 
GCCCTCACCA TCTTCCAGTC CATCGAATAT GCTGATGAAC AAGGCCAAAT GCAGTTATCC 
ACCTTCTTTT CCTTCATGTT GGAAAACTAC ACACATATAC ATAAGGAAGA GCTAGAATTA 
AGAAATCAGT CTCTTGAAAG CGAACAGGAC ATGAGGGATA GATGGGATTA TGTGGACTCG 
ATAGATGTCC CAGACTCCTA TAATGGTCCT CGGCTACAAT TTCCTCTCAC TTGTACGGAT 
ATTGATTTAC TTCTTGAGGC CTTCAAGGAA CAACAGATAC TTCATGCCCA TTATGTCTTA 
GAGGTGCTAT TTGAAACCAA GAAAGTCCTG AAGCAAATGC CGAATTTCAC TCACATACAA 
ACTTCTCCCT CCAAAGAGGT AACAATCTGT GGTGATTTGC ATGGGAAACT GGATGATCTT 
TTTTTGATCT TCTACAAGAA TGGTCTCCCC TCAGAGAGGA ACCCGTATGT TTTTAATGGT 
GACTTTGTAG ATCGAGGAAA GAATTCCATA GAGATCCTAA TGATCCTGTG TGTGAGTTTT 
CTTGTCTACC CCAATGACCT GCACTTGAAC AGAGGGAACC ACGAAGATTT TATGATGAAT 
CTGAGGTATG GCTTCACGAA AGAAATTTTG CATAAATATA AGCTACATGG AAAAAGAATC 
TTACAAATCT TGGAAGAATT CTATGCCTGG CTCCCAATCG GTACAATCGT TGACAATGAA 
ATCCTGGTCA TCCATGGTGG GATATCAGAG ACCACAGACT TGAATTTACT CCACCGTGTA 
GAGAGGAACA AGATGAAATC TGTGCTGATA CCACCAACGG AAACAAACAG AGACCATGAC 
ACTGACTCGA AGCACAATAA AGTAGGTGTG ACTTTTAATG CACATGGAAG AATCAAAACA 
AATGGATCTC CTACTGAACA CTTAACAGAG CATGAATGGG AACAGATTAT TGATATTCTG 
TGGAGTGATC CCAGAGGCAA AAATGGCTGT TTTCCAAATA CGTGCCGAGG AGGGGGCTGC 
TATTTTGGAC CAGATGTTAC TTCCAAGATT CTTAATAAAT ACCAGTTGAA GATGCTCATC 
AGGTCTCATG AATGTAAGCC CGAAGGGTAT GAAATCTGTC ATGATGGGAA GGTGGTGACT 
ATATTTTCTG CTTCTAATTA TTATGAAGAA GGCAGCAATC GAGGAGCTTA CATCAAACTA 
TGTTCTGGTA CAACTCCTCG ATTTTTCCAG TACCAAGTAA CTAAAGCAAC GTGCTTTCAG 
CCTCTTCGCC AAAGAGTGGA TACTATGGAA AACAGCGCCA TCAAGATATT AAGAGAGAGA 
GTGATTTCAC GAAAAAGTGA CCTTACTCGT GCTTTCCAAC TTCAAGACCA CAGAAAATCA 
GGAAAACTTT CTGTGAGCCA GTGGGCTTTT TGCATGGAGA ACATTTTGGG GCTGAACTTA 
CCATGGAGAT CCCTCAGTTC GAATCTGGTA AACATAGACC AAAATGGAAA CGTTGAATAC 
ATGTCCAGCT TCCAGAATAT CCGCATTGAA AAACCTGTAC AAGAGGCTCA TTCTACTCTA 
GTTGAAACTC TGTACAGATA CAGATCTGAC CTGGAAATCA TATTTAATGC CATTGACACT 
GATCACTCAG GCCTGATCTC CGTGGAAGAA TTTCGTGCCA TGTGGAAACT TTTTAGTTCT 
CACTACAATG TTCACATTGA TGATTCCCAA GTCAATAAGC TTGCCAACAT AATGGACTTG 
AACAAAGATG GAAGCATTGA CTTTAATGAG TTTTTAAAGG CTTTCTATGT AGTGCATAGA 
TATGAAGACT TGATGAAACC TGATGTCACC AACCTTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274610.1
CDS	809..2770
Translation	MGCSSSSTKTRRSDTSLRAALIIQNWYRGYKARLKARQHYALTIFQSIEYADEQGQMQLSTFFSFMLENYTHIHKEELELRNQSLESEQDMRDRWDYVDSIDVPDSYNGPRLQFPLTCTDIDLLLEAFKEQQILHAHYVLEVLFETKKVLKQMPNFTHIQTSPSKEVTICGDLHGKLDDLFLIFYKNGLPSERNPYVFNGDFVDRGKNSIEILMILCVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKLHGKRILQILEEFYAWLPIGTIVDNEILVIHGGISETTDLNLLHRVERNKMKSVLIPPTETNRDHDTDSKHNKVGVTFNAHGRIKTNGSPTEHLTEHEWEQIIDILWSDPRGKNGCFPNTCRGGGCYFGPDVTSKILNKYQLKMLIRSHECKPEGYEICHDGKVVTIFSASNYYEEGSNRGAYIKLCSGTTPRFFQYQVTKATCFQPLRQRVDTMENSAIKILRERVISRKSDLTRAFQLQDHRKSGKLSVSQWAFCMENILGLNLPWRSLSSNLVNIDQNGNVEYMSSFQNIRIEKPVQEAHSTLVETLYRYRSDLEIIFNAIDTDHSGLISVEEFRAMWKLFSSHYNVHIDDSQVNKLANIMDLNKDGSIDFNEFLKAFYVVHRYEDLMKPDVTNLG

Target ORF information:

RefSeq Version	XM_005274553.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274553.2

ORF Insert Sequence:

ATGGGATGCA GCAGTTCTTC AACGAAAACC AGGAGATCTG ACACATCACT GAGAGCTGCG 
TTGATCATCC AGAACTGGTA CCGAGGTTAC AAAGCTCGAC TGAAGGCCAG ACAACACTAT 
GCCCTCACCA TCTTCCAGTC CATCGAATAT GCTGATGAAC AAGGCCAAAT GCAGTTATCC 
ACCTTCTTTT CCTTCATGTT GGAAAACTAC ACACATATAC ATAAGGAAGA GCTAGAATTA 
AGAAATCAGT CTCTTGAAAG CGAACAGGAC ATGAGGGATA GATGGGATTA TGTGGACTCG 
ATAGATGTCC CAGACTCCTA TAATGGTCCT CGGCTACAAT TTCCTCTCAC TTGTACGGAT 
ATTGATTTAC TTCTTGAGGC CTTCAAGGAA CAACAGATAC TTCATGCCCA TTATGTCTTA 
GAGGTGCTAT TTGAAACCAA GAAAGTCCTG AAGCAAATGC CGAATTTCAC TCACATACAA 
ACTTCTCCCT CCAAAGAGGT AACAATCTGT GGTGATTTGC ATGGGAAACT GGATGATCTT 
TTTTTGATCT TCTACAAGAA TGGTCTCCCC TCAGAGAGGA ACCCGTATGT TTTTAATGGT 
GACTTTGTAG ATCGAGGAAA GAATTCCATA GAGATCCTAA TGATCCTGTG TGTGAGTTTT 
CTTGTCTACC CCAATGACCT GCACTTGAAC AGAGGGAACC ACGAAGATTT TATGATGAAT 
CTGAGGTATG GCTTCACGAA AGAAATTTTG CATAAATATA AGCTACATGG AAAAAGAATC 
TTACAAATCT TGGAAGAATT CTATGCCTGG CTCCCAATCG GTACAATCGT TGACAATGAA 
ATCCTGGTCA TCCATGGTGG GATATCAGAG ACCACAGACT TGAATTTACT CCACCGTGTA 
GAGAGGAACA AGATGAAATC TGTGCTGATA CCACCAACGG AAACAAACAG AGACCATGAC 
ACTGACTCGA AGCACAATAA AGTAGGTGTG ACTTTTAATG CACATGGAAG AATCAAAACA 
AATGGATCTC CTACTGAACA CTTAACAGAG CATGAATGGG AACAGATTAT TGATATTCTG 
TGGAGTGATC CCAGAGGCAA AAATGGCTGT TTTCCAAATA CGTGCCGAGG AGGGGGCTGC 
TATTTTGGAC CAGATGTTAC TTCCAAGATT CTTAATAAAT ACCAGTTGAA GATGCTCATC 
AGGTCTCATG AATGTAAGCC CGAAGGGTAT GAAATCTGTC ATGATGGGAA GGTGGTGACT 
ATATTTTCTG CTTCTAATTA TTATGAAGAA GGCAGCAATC GAGGAGCTTA CATCAAACTA 
TGTTCTGGTA CAACTCCTCG ATTTTTCCAG TACCAAGTAA CTAAAGCAAC GTGCTTTCAG 
CCTCTTCGCC AAAGAGTGGA TACTATGGAA AACAGCGCCA TCAAGATATT AAGAGAGAGA 
GTGATTTCAC GAAAAAGTGA CCTTACTCGT GCTTTCCAAC TTCAAGACCA CAGAAAATCA 
GGAAAACTTT CTGTGAGCCA GTGGGCTTTT TGCATGGAGA ACATTTTGGG GCTGAACTTA 
CCATGGAGAT CCCTCAGTTC GAATCTGGTA AACATAGACC AAAATGGAAA CGTTGAATAC 
ATGTCCAGCT TCCAGAATAT CCGCATTGAA AAACCTGTAC AAGAGGCTCA TTCTACTCTA 
GTTGAAACTC TGTACAGATA CAGATCTGAC CTGGAAATCA TATTTAATGC CATTGACACT 
GATCACTCAG GCCTGATCTC CGTGGAAGAA TTTCGTGCCA TGTGGAAACT TTTTAGTTCT 
CACTACAATG TTCACATTGA TGATTCCCAA GTCAATAAGC TTGCCAACAT AATGGACTTG 
AACAAAGATG GAAGCATTGA CTTTAATGAG TTTTTAAAGG CTTTCTATGT AGTGCATAGA 
TATGAAGACT TGATGAAACC TGATGTCACC AACCTTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( XM_017029612.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885101.1 Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu53937
Clone ID Related Accession (Same CDS sequence)	XM_017029612.1
Accession Version	XM_017029612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase with EF-hands 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGATA GATGGGATTA TGTGGACTCG ATAGATGTCC CAGACTCCTA TAATGGTCCT 
CGGCTACAAT TTCCTCTCAC TTGTACGGAT ATTGATTTAC TTCTTGAGGC CTTCAAGGAA 
CAACAGATAC TTCATGCCCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAAGTCCTG 
AAGCAAATGC CGAATTTCAC TCACATACAA ACTTCTCCCT CCAAAGAGGT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTACAAGAA TGGTCTCCCC 
TCAGAGAGGA ACCCGTATGT TTTTAATGGT GACTTTGTAG ATCGAGGAAA GAATTCCATA 
GAGATCCTAA TGATCCTGTG TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ACGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACGAA AGAAATTTTG 
CATAAATATA AGCTACATGG AAAAAGAATC TTACAAATCT TGGAAGAATT CTATGCCTGG 
CTCCCAATCG GTACAATCGT TGACAATGAA ATCCTGGTCA TCCATGGTGG GATATCAGAG 
ACCACAGACT TGAATTTACT CCACCGTGTA GAGAGGAACA AGATGAAATC TGTGCTGATA 
CCACCAACGG AAACAAACAG AGACCATGAC ACTGACTCGA AGCACAATAA AGTAGGTGTG 
ACTTTTAATG CACATGGAAG AATCAAAACA AATGGATCTC CTACTGAACA CTTAACAGAG 
CATGAATGGG AACAGATTAT TGATATTCTG TGGAGTGATC CCAGAGGCAA AAATGGCTGT 
TTTCCAAATA CGTGCCGAGG AGGGGGCTGC TATTTTGGAC CAGATGTTAC TTCCAAGATT 
CTTAATAAAT ACCAGTTGAA GATGCTCATC AGGTCTCATG AATGTAAGCC CGAAGGGTAT 
GAAATCTGTC ATGATGGGAA GGTGGTGACT ATATTTTCTG CTTCTAATTA TTATGAAGAA 
GGCAGCAATC GAGGAGCTTA CATCAAACTA TGTTCTGGTA CAACTCCTCG ATTTTTCCAG 
TACCAAGTAA CTAAAGCAAC GTGCTTTCAG CCTCTTCGCC AAAGAGTGGA TACTATGGAA 
AACAGCGCCA TCAAGATATT AAGAGAGAGA GTGATTTCAC GAAAAAGTGA CCTTACTCGT 
GCTTTCCAAC TTCAAGACCA CAGAAAATCA GGAAAACTTT CTGTGAGCCA GTGGGCTTTT 
TGCATGGAGA ACATTTTGGG GCTGAACTTA CCATGGAGAT CCCTCAGTTC GAATCTGGTA 
AACATAGACC AAAATGGAAA CGTTGAATAC ATGTCCAGCT TCCAGAATAT CCGCATTGAA 
AAACCTGTAC AAGAGGCTCA TTCTACTCTA GTTGAAACTC TGTACAGATA CAGATCTGAC 
CTGGAAATCA TATTTAATGC CATTGACACT GATCACTCAG GCCTGATCTC CGTGGAAGAA 
TTTCGTGCCA TGTGGAAACT TTTTAGTTCT CACTACAATG TTCACATTGA TGATTCCCAA 
GTCAATAAGC TTGCCAACAT AATGGACTTG AACAAAGATG GAAGCATTGA CTTTAATGAG 
TTTTTAAAGG CTTTCTATGT AGTGCATAGA TATGAAGACT TGATGAAACC TGATGTCACC 
AACCTTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885101.1
CDS	890..2581
Translation	MRDRWDYVDSIDVPDSYNGPRLQFPLTCTDIDLLLEAFKEQQILHAHYVLEVLFETKKVLKQMPNFTHIQTSPSKEVTICGDLHGKLDDLFLIFYKNGLPSERNPYVFNGDFVDRGKNSIEILMILCVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKLHGKRILQILEEFYAWLPIGTIVDNEILVIHGGISETTDLNLLHRVERNKMKSVLIPPTETNRDHDTDSKHNKVGVTFNAHGRIKTNGSPTEHLTEHEWEQIIDILWSDPRGKNGCFPNTCRGGGCYFGPDVTSKILNKYQLKMLIRSHECKPEGYEICHDGKVVTIFSASNYYEEGSNRGAYIKLCSGTTPRFFQYQVTKATCFQPLRQRVDTMENSAIKILRERVISRKSDLTRAFQLQDHRKSGKLSVSQWAFCMENILGLNLPWRSLSSNLVNIDQNGNVEYMSSFQNIRIEKPVQEAHSTLVETLYRYRSDLEIIFNAIDTDHSGLISVEEFRAMWKLFSSHYNVHIDDSQVNKLANIMDLNKDGSIDFNEFLKAFYVVHRYEDLMKPDVTNLG

Target ORF information:

RefSeq Version	XM_017029612.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029612.1

ORF Insert Sequence:

ATGAGGGATA GATGGGATTA TGTGGACTCG ATAGATGTCC CAGACTCCTA TAATGGTCCT 
CGGCTACAAT TTCCTCTCAC TTGTACGGAT ATTGATTTAC TTCTTGAGGC CTTCAAGGAA 
CAACAGATAC TTCATGCCCA TTATGTCTTA GAGGTGCTAT TTGAAACCAA GAAAGTCCTG 
AAGCAAATGC CGAATTTCAC TCACATACAA ACTTCTCCCT CCAAAGAGGT AACAATCTGT 
GGTGATTTGC ATGGGAAACT GGATGATCTT TTTTTGATCT TCTACAAGAA TGGTCTCCCC 
TCAGAGAGGA ACCCGTATGT TTTTAATGGT GACTTTGTAG ATCGAGGAAA GAATTCCATA 
GAGATCCTAA TGATCCTGTG TGTGAGTTTT CTTGTCTACC CCAATGACCT GCACTTGAAC 
AGAGGGAACC ACGAAGATTT TATGATGAAT CTGAGGTATG GCTTCACGAA AGAAATTTTG 
CATAAATATA AGCTACATGG AAAAAGAATC TTACAAATCT TGGAAGAATT CTATGCCTGG 
CTCCCAATCG GTACAATCGT TGACAATGAA ATCCTGGTCA TCCATGGTGG GATATCAGAG 
ACCACAGACT TGAATTTACT CCACCGTGTA GAGAGGAACA AGATGAAATC TGTGCTGATA 
CCACCAACGG AAACAAACAG AGACCATGAC ACTGACTCGA AGCACAATAA AGTAGGTGTG 
ACTTTTAATG CACATGGAAG AATCAAAACA AATGGATCTC CTACTGAACA CTTAACAGAG 
CATGAATGGG AACAGATTAT TGATATTCTG TGGAGTGATC CCAGAGGCAA AAATGGCTGT 
TTTCCAAATA CGTGCCGAGG AGGGGGCTGC TATTTTGGAC CAGATGTTAC TTCCAAGATT 
CTTAATAAAT ACCAGTTGAA GATGCTCATC AGGTCTCATG AATGTAAGCC CGAAGGGTAT 
GAAATCTGTC ATGATGGGAA GGTGGTGACT ATATTTTCTG CTTCTAATTA TTATGAAGAA 
GGCAGCAATC GAGGAGCTTA CATCAAACTA TGTTCTGGTA CAACTCCTCG ATTTTTCCAG 
TACCAAGTAA CTAAAGCAAC GTGCTTTCAG CCTCTTCGCC AAAGAGTGGA TACTATGGAA 
AACAGCGCCA TCAAGATATT AAGAGAGAGA GTGATTTCAC GAAAAAGTGA CCTTACTCGT 
GCTTTCCAAC TTCAAGACCA CAGAAAATCA GGAAAACTTT CTGTGAGCCA GTGGGCTTTT 
TGCATGGAGA ACATTTTGGG GCTGAACTTA CCATGGAGAT CCCTCAGTTC GAATCTGGTA 
AACATAGACC AAAATGGAAA CGTTGAATAC ATGTCCAGCT TCCAGAATAT CCGCATTGAA 
AAACCTGTAC AAGAGGCTCA TTCTACTCTA GTTGAAACTC TGTACAGATA CAGATCTGAC 
CTGGAAATCA TATTTAATGC CATTGACACT GATCACTCAG GCCTGATCTC CGTGGAAGAA 
TTTCGTGCCA TGTGGAAACT TTTTAGTTCT CACTACAATG TTCACATTGA TGATTCCCAA 
GTCAATAAGC TTGCCAACAT AATGGACTTG AACAAAGATG GAAGCATTGA CTTTAATGAG 
TTTTTAAAGG CTTTCTATGT AGTGCATAGA TATGAAGACT TGATGAAACC TGATGTCACC 
AACCTTGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( NM_152226.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_689412.1 Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant 3, mRNA.

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CloneID	OHu06687
Clone ID Related Accession (Same CDS sequence)	NM_152226.1
Accession Version	NM_152226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase with EF-hands 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X97867.1. Transcript Variant: This variant (3) lacks an in-frame exon in the coding region compared to variant 1, resulting in an isoform (3) that is missing an internal segment compared to isoform 1. Isoform 3 contains a catalytic domain and two EF-hand motifs. Its functional significance is unknown. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1803617.257481.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGATGCA GCAGTTCTTC AACGAAAACC AGGAGATCTG ACACATCACT GAGAGCTGCG 
TTGATCATCC AGAACTGGTA CCGAGGTTAC AAAGCTCGAC TGAAGGCCAG ACAACACTAT 
GCCCTCACCA TCTTCCAGTC CATCGAATAT GCTGATGAAC AAGGCCAAAT GCAGTTATCC 
ACCTTCTTTT CCTTCATGTT GGAAAACTAC ACACATATAC ATAAGGAAGA GCTAGAATTA 
AGAAATCAGT CTCTTGAAAG CGAACAGGAC ATGAGGGATA GATGGGATTA TGTGGACTCG 
ATAGATGTCC CAGACTCCTA TAATGGTCCT CGGCTACAAT TTCCTCTCAC TTGTACGGAT 
ATTGATTTAC TTCTTGAGGC CTTCAAGGAA CAACAGATAC TTCATGCCCA TTATGTCTTA 
GAGGTGCTAT TTGAAACCAA GAAAGTCCTG AAGCAAATGC CGAATTTCAC TCACATACAA 
ACTTCTCCCT CCAAAGAGGT AACAATCTGT GGTGATTTGC ATGGGAAACT GGATGATCTT 
TTTTTGATCT TCTACAAGAA TGGTCTCCCC TCAGAGAGGA ACCCGTATGT TTTTAATGGT 
GACTTTGTAG ATCGAGGAAA GAATTCCATA GAGATCCTAA TGATCCTGTG TGTGAGTTTT 
CTTGTCTACC CCAATGACCT GCACTTGAAC AGAGGGAACC ACGAAGATTT TATGATGAAT 
CTGAGGTATG GCTTCACGAA AGAAATTTTG CATAAATATA AGCTACATGG AAAAAGAATC 
TTACAAATCT TGGAAGAATT CTATGCCTGG CTCCCAATCG GTACAATCGT TGACAATGAA 
ATCCTGGTCA TCCATGGTGG GATATCAGAG ACCACAGACT TGAATTTACT CCACCGTGTA 
GAGAGGAACA AGATGAAATC TGTGCTGATA CCACCAACGG AAACAAACAG AGACCATGAC 
ACTGACTCGA AGCACAATAA AGTAGGTGTG ACTTTTAATG CACATGGAAG AATCAAAACA 
AATGGATCTC CTACTGAACA CTTAACAGAG CATGAATGGG AACAGGTGGT GACTATATTT 
TCTGCTTCTA ATTATTATGA AGAAGGCAGC AATCGAGGAG CTTACATCAA ACTATGTTCT 
GGTACAACTC CTCGATTTTT CCAGTACCAA GTAACTAAAG CAACGTGCTT TCAGCCTCTT 
CGCCAAAGAG TGGATACTAT GGAAAACAGC GCCATCAAGA TATTAAGAGA GAGAGTGATT 
TCACGAAAAA GTGACCTTAC TCGTGCTTTC CAACTTCAAG ACCACAGAAA ATCAGGAAAA 
CTTTCTGTGA GCCAGTGGGC TTTTTGCATG GAGAACATTT TGGGGCTGAA CTTACCATGG 
AGATCCCTCA GTTCGAATCT GGTAAACATA GACCAAAATG GAAACGTTGA ATACATGTCC 
AGCTTCCAGA ATATCCGCAT TGAAAAACCT GTACAAGAGG CTCATTCTAC TCTAGTTGAA 
ACTCTGTACA GATACAGATC TGACCTGGAA ATCATATTTA ATGCCATTGA CACTGATCAC 
TCAGGCCTGA TCTCCGTGGA AGAATTTCGT GCCATGTGGA AACTTTTTAG TTCTCACTAC 
AATGTTCACA TTGATGATTC CCAAGTCAAT AAGCTTGCCA ACATAATGGA CTTGAACAAA 
GATGGAAGCA TTGACTTTAA TGAGTTTTTA AAGGCTTTCT ATGTAGTGCA TAGATATGAA 
GACTTGATGA AACCTGATGT CACCAACCTT GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689412.1
CDS	482..2257
Translation	MGCSSSSTKTRRSDTSLRAALIIQNWYRGYKARLKARQHYALTIFQSIEYADEQGQMQLSTFFSFMLENYTHIHKEELELRNQSLESEQDMRDRWDYVDSIDVPDSYNGPRLQFPLTCTDIDLLLEAFKEQQILHAHYVLEVLFETKKVLKQMPNFTHIQTSPSKEVTICGDLHGKLDDLFLIFYKNGLPSERNPYVFNGDFVDRGKNSIEILMILCVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKLHGKRILQILEEFYAWLPIGTIVDNEILVIHGGISETTDLNLLHRVERNKMKSVLIPPTETNRDHDTDSKHNKVGVTFNAHGRIKTNGSPTEHLTEHEWEQVVTIFSASNYYEEGSNRGAYIKLCSGTTPRFFQYQVTKATCFQPLRQRVDTMENSAIKILRERVISRKSDLTRAFQLQDHRKSGKLSVSQWAFCMENILGLNLPWRSLSSNLVNIDQNGNVEYMSSFQNIRIEKPVQEAHSTLVETLYRYRSDLEIIFNAIDTDHSGLISVEEFRAMWKLFSSHYNVHIDDSQVNKLANIMDLNKDGSIDFNEFLKAFYVVHRYEDLMKPDVTNLG

Target ORF information:

RefSeq Version	NM_152226.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase with EF-hand domain 1 (PPEF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152226.1

ORF Insert Sequence:

ATGGGATGCA GCAGTTCTTC AACGAAAACC AGGAGATCTG ACACATCACT GAGAGCTGCG 
TTGATCATCC AGAACTGGTA CCGAGGTTAC AAAGCTCGAC TGAAGGCCAG ACAACACTAT 
GCCCTCACCA TCTTCCAGTC CATCGAATAT GCTGATGAAC AAGGCCAAAT GCAGTTATCC 
ACCTTCTTTT CCTTCATGTT GGAAAACTAC ACACATATAC ATAAGGAAGA GCTAGAATTA 
AGAAATCAGT CTCTTGAAAG CGAACAGGAC ATGAGGGATA GATGGGATTA TGTGGACTCG 
ATAGATGTCC CAGACTCCTA TAATGGTCCT CGGCTACAAT TTCCTCTCAC TTGTACGGAT 
ATTGATTTAC TTCTTGAGGC CTTCAAGGAA CAACAGATAC TTCATGCCCA TTATGTCTTA 
GAGGTGCTAT TTGAAACCAA GAAAGTCCTG AAGCAAATGC CGAATTTCAC TCACATACAA 
ACTTCTCCCT CCAAAGAGGT AACAATCTGT GGTGATTTGC ATGGGAAACT GGATGATCTT 
TTTTTGATCT TCTACAAGAA TGGTCTCCCC TCAGAGAGGA ACCCGTATGT TTTTAATGGT 
GACTTTGTAG ATCGAGGAAA GAATTCCATA GAGATCCTAA TGATCCTGTG TGTGAGTTTT 
CTTGTCTACC CCAATGACCT GCACTTGAAC AGAGGGAACC ACGAAGATTT TATGATGAAT 
CTGAGGTATG GCTTCACGAA AGAAATTTTG CATAAATATA AGCTACATGG AAAAAGAATC 
TTACAAATCT TGGAAGAATT CTATGCCTGG CTCCCAATCG GTACAATCGT TGACAATGAA 
ATCCTGGTCA TCCATGGTGG GATATCAGAG ACCACAGACT TGAATTTACT CCACCGTGTA 
GAGAGGAACA AGATGAAATC TGTGCTGATA CCACCAACGG AAACAAACAG AGACCATGAC 
ACTGACTCGA AGCACAATAA AGTAGGTGTG ACTTTTAATG CACATGGAAG AATCAAAACA 
AATGGATCTC CTACTGAACA CTTAACAGAG CATGAATGGG AACAGGTGGT GACTATATTT 
TCTGCTTCTA ATTATTATGA AGAAGGCAGC AATCGAGGAG CTTACATCAA ACTATGTTCT 
GGTACAACTC CTCGATTTTT CCAGTACCAA GTAACTAAAG CAACGTGCTT TCAGCCTCTT 
CGCCAAAGAG TGGATACTAT GGAAAACAGC GCCATCAAGA TATTAAGAGA GAGAGTGATT 
TCACGAAAAA GTGACCTTAC TCGTGCTTTC CAACTTCAAG ACCACAGAAA ATCAGGAAAA 
CTTTCTGTGA GCCAGTGGGC TTTTTGCATG GAGAACATTT TGGGGCTGAA CTTACCATGG 
AGATCCCTCA GTTCGAATCT GGTAAACATA GACCAAAATG GAAACGTTGA ATACATGTCC 
AGCTTCCAGA ATATCCGCAT TGAAAAACCT GTACAAGAGG CTCATTCTAC TCTAGTTGAA 
ACTCTGTACA GATACAGATC TGACCTGGAA ATCATATTTA ATGCCATTGA CACTGATCAC 
TCAGGCCTGA TCTCCGTGGA AGAATTTCGT GCCATGTGGA AACTTTTTAG TTCTCACTAC 
AATGTTCACA TTGATGATTC CCAAGTCAAT AAGCTTGCCA ACATAATGGA CTTGAACAAA 
GATGGAAGCA TTGACTTTAA TGAGTTTTTA AAGGCTTTCT ATGTAGTGCA TAGATATGAA 
GACTTGATGA AACCTGATGT CACCAACCTT GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein serine/threonine phosphatase PPEF-1 suppresses genotoxic stress response via dephosphorylation of PDCD5.
Scientific reports739222(2017 Jan)
Park SY,Seo J,Choi HK,Oh HJ,Guk G,Lee YH,Lee J,Jun WJ,Choi KC,Yoon HG

Molecular cloning of a novel PPEF-1 gene variant from a T-cell lymphoblastic lymphoma cell line.
Recent patents on DNA & gene sequences6(1)72-7(2012 Apr)
Ho P,Dai KS,Chen HL

The DNA sequence of the human X chromosome.
Nature434(7031)325-37(2005 Mar)
Ross MT,Grafham DV,Coffey AJ,Scherer S,McLay K,Muzny D,Platzer M,Howell GR,Burrows C,Bird CP,Frankish A,Lovell FL,Howe KL,Ashurst JL,Fulton RS,Sudbrak R,Wen G,Jones MC,Hurles ME,Andrews TD,Scott CE,Searle S,Ramser J,Whittaker A,Deadman R,Carter NP,Hunt SE,Chen R,Cree A,Gunaratne P,Havlak P,Hodgson A,Metzker ML,Richards S,Scott G,Steffen D,Sodergren E,Wheeler DA,Worley KC,Ainscough R,Ambrose KD,Ansari-Lari MA,Aradhya S,Ashwell RI,Babbage AK,Bagguley CL,Ballabio A,Banerjee R,Barker GE,Barlow KF,Barrett IP,Bates KN,Beare DM,Beasley H,Beasley O,Beck A,Bethel G,Blechschmidt K,Brady N,Bray-Allen S,Bridgeman AM,Brown AJ,Brown MJ,Bonnin D,Bruford EA,Buhay C,Burch P,Burford D,Burgess J,Burrill W,Burton J,Bye JM,Carder C,Carrel L,Chako J,Chapman JC,Chavez D,Chen E,Chen G,Chen Y,Chen Z,Chinault C,Ciccodicola A,Clark SY,Clarke G,Clee CM,Clegg S,Clerc-Blankenburg K,Clifford K,Cobley V,Cole CG,Conquer JS,Corby N,Connor RE,David R,Davies J,Davis C,Davis J,Delgado O,Deshazo D,Dhami P,Ding Y,Dinh H,Dodsworth S,Draper H,Dugan-Rocha S,Dunham A,Dunn M,Durbin KJ,Dutta I,Eades T,Ellwood M,Emery-Cohen A,Errington H,Evans KL,Faulkner L,Francis F,Frankland J,Fraser AE,Galgoczy P,Gilbert J,Gill R,Gl?ckner G,Gregory SG,Gribble S,Griffiths C,Grocock R,Gu Y,Gwilliam R,Hamilton C,Hart EA,Hawes A,Heath PD,Heitmann K,Hennig S,Hernandez J,Hinzmann B,Ho S,Hoffs M,Howden PJ,Huckle EJ,Hume J,Hunt PJ,Hunt AR,Isherwood J,Jacob L,Johnson D,Jones S,de Jong PJ,Joseph SS,Keenan S,Kelly S,Kershaw JK,Khan Z,Kioschis P,Klages S,Knights AJ,Kosiura A,Kovar-Smith C,Laird GK,Langford C,Lawlor S,Leversha M,Lewis L,Liu W,Lloyd C,Lloyd DM,Loulseged H,Loveland JE,Lovell JD,Lozado R,Lu J,Lyne R,Ma J,Maheshwari M,Matthews LH,McDowall J,McLaren S,McMurray A,Meidl P,Meitinger T,Milne S,Miner G,Mistry SL,Morgan M,Morris S,M?ller I,Mullikin JC,Nguyen N,Nordsiek G,Nyakatura G,O'Dell CN,Okwuonu G,Palmer S,Pandian R,Parker D,Parrish J,Pasternak S,Patel D,Pearce AV,Pearson DM,Pelan SE,Perez L,Porter KM,Ramsey Y,Reichwald K,Rhodes S,Ridler KA,Schlessinger D,Schueler MG,Sehra HK,Shaw-Smith C,Shen H,Sheridan EM,Shownkeen R,Skuce CD,Smith ML,Sotheran EC,Steingruber HE,Steward CA,Storey R,Swann RM,Swarbreck D,Tabor PE,Taudien S,Taylor T,Teague B,Thomas K,Thorpe A,Timms K,Tracey A,Trevanion S,Tromans AC,d'Urso M,Verduzco D,Villasana D,Waldron L,Wall M,Wang Q,Warren J,Warry GL,Wei X,West A,Whitehead SL,Whiteley MN,Wilkinson JE,Willey DL,Williams G,Williams L,Williamson A,Williamson H,Wilming L,Woodmansey RL,Wray PW,Yen J,Zhang J,Zhou J,Zoghbi H,Zorilla S,Buck D,Reinhardt R,Poustka A,Rosenthal A,Lehrach H,Meindl A,Minx PJ,Hillier LW,Willard HF,Wilson RK,Waterston RH,Rice CM,Vaudin M,Coulson A,Nelson DL,Weinstock G,Sulston JE,Durbin R,Hubbard T,Gibbs RA,Beck S,Rogers J,Bentley DR

An unappreciated role for RNA surveillance.
Genome biology5(2)R8(2004)
Hillman RT,Green RE,Brenner SE

Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.
Nature biotechnology21(5)566-9(2003 May)
Gevaert K,Goethals M,Martens L,Van Damme J,Staes A,Thomas GR,Vandekerckhove J

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PPEF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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