DDX56 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX56
Entrez Gene ID	54606
Full Name	DEAD-box helicase 56
Synonyms	DDX21,DDX26,NOH61
General protein information	Preferred Names DEAD-box helicase 56 Names probable ATP-dependent RNA helicase DDX56 61-kd nucleolar helicase ATP-dependent 61 kDa nucleolar RNA helicase DEAD (Asp-Glu-Ala-Asp) box helicase 56 DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 DEAD box protein 21 DEAD box protein 56 DEAD-box RNA helicase nucleolar helicase of 61 kDa putative nucleolar RNA helicase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p13
Summary	This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012].
Disorder MIM:	608023

mRNA and Protein(s)

mRNA	Protein	Name
NM_001257189.1	NP_001244118.1	probable ATP-dependent RNA helicase DDX56 isoform 2
NM_019082.3	NP_061955.1	probable ATP-dependent RNA helicase DDX56 isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ddx56	NP_080814.1
Magnaporthe oryzae (rice blast fungus)	MGG_04993	XP_003712493.1
Arabidopsis thaliana (thale cress)	AT4G34910	NP_195217.1
Bos taurus (cattle)	DDX56	NP_001029501.1
Drosophila melanogaster (fruit fly)	Hlc	NP_523434.2
Saccharomyces cerevisiae (baker's yeast)	DBP9	NP_013378.1
Schizosaccharomyces pombe (fission yeast)	SPCC1494.06c	NP_588531.1
Rattus norvegicus (Norway rat)	Ddx56	NP_001004211.1
Danio rerio (zebrafish)	ddx56	NP_001003876.1
Homo sapiens (human)	DDX56	NP_061955.1
Canis lupus familiaris (dog)	DDX56	XP_005642242.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011147	XP_564375.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011145	XP_309500.4
Caenorhabditis elegans (roundworm)	CELE_C24H12.4	NP_740966.1
Eremothecium gossypii	AGOS_AGR278C	NP_986944.1
Oryza sativa (rice)	Os03g0728800	NP_001051149.1
Xenopus tropicalis (tropical clawed frog)	ddx56	NP_001120553.1
Pan troglodytes (chimpanzee)	DDX56	XP_001145021.1
	DDX56	XP_001089984.2
Kluyveromyces lactis	KLLA0C04576g	XP_452403.1
Neurospora crassa	NCU07070	XP_960335.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleolar helicase DDX56 redistributes to West Nile virus assembly sites.
Reid CR, Hobman TC
Virology500169-177(2017 01)

The helicase activity of DDX56 is required for its role in assembly of infectious West Nile virus particles.
Xu Z, Hobman TC
Virology433(1)226-35(2012 Nov)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

West Nile virus infection results in relocalization of DDX56 from nucleoli to virus assembly sites on the endoplasmic reticululm (ER), an observation that is consistent with a role for DDX56 in WNV virion assembly.
Title: The nucleolar helicase DDX56 redistributes to West Nile virus assembly sites.

The helicase activity of DDX56 is required for its role in assembly of infectious West Nile virus particles.
Title: The helicase activity of DDX56 is required for its role in assembly of infectious West Nile virus particles.

Coimmunoprecipitation confirmed that capsid protein binds to human DDX56 in infected cells and that this interaction is required for assembly of infectious West Nile virus particles.
Title: The capsid-binding nucleolar helicase DDX56 is important for infectivity of West Nile virus.

DDX26 maps to7p12, a region of frequent chromosome amplifications in glioblastoma involving the EGFR gene.
Title: Molecular characterization of DDX26, a human DEAD-box RNA helicase, located on chromosome 7p12.

The following DDX56 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX56 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu02432	NM_019082.3 Latest version!	Homo sapiens DEAD-box helicase 56 (DDX56), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu08291	NM_001257189.1 Latest version!	Homo sapiens DEAD-box helicase 56 (DDX56), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX56 ( NM_019082.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_061955.1 Homo sapiens DEAD-box helicase 56 (DDX56), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02432
Clone ID Related Accession (Same CDS sequence)	NM_019082.3
Accession Version	NM_019082.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX56 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM787430.1, AK125595.1, BC001235.1 and CA423604.1. On Mar 17, 2012 this sequence version replaced NM_019082.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.114397.1, SRR1803615.43704.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGACT CTGAAGCACT GGGCTTCGAA CACATGGGCC TCGATCCCCG GCTCCTTCAG 
GCTGTCACCG ATCTGGGCTG GTCGCGACCT ACGCTGATCC AGGAGAAGGC CATCCCACTG 
GCCCTAGAAG GGAAGGACCT CCTGGCTCGG GCCCGCACGG GCTCCGGGAA GACGGCCGCT 
TATGCTATTC CGATGCTGCA GCTGTTGCTC CATAGGAAGG CGACAGGTCC GGTGGTAGAA 
CAGGCAGTGA GAGGCCTTGT TCTTGTTCCT ACCAAGGAGC TGGCACGGCA AGCACAGTCC 
ATGATTCAGC AGCTGGCTAC CTACTGTGCT CGGGATGTCC GAGTGGCCAA TGTCTCAGCT 
GCTGAAGACT CAGTCTCTCA GAGAGCTGTG CTGATGGAGA AGCCAGATGT GGTAGTAGGG 
ACCCCATCTC GCATATTAAG CCACTTGCAG CAAGACAGCC TGAAACTTCG TGACTCCCTG 
GAGCTTTTGG TGGTGGACGA AGCTGACCTT CTTTTTTCCT TTGGCTTTGA AGAAGAGCTC 
AAGAGTCTCC TCTGTCACTT GCCCCGGATT TACCAGGCTT TTCTCATGTC AGCTACTTTT 
AACGAGGACG TACAAGCACT CAAGGAGCTG ATATTACATA ACCCGGTTAC CCTTAAGTTA 
CAGGAGTCCC AGCTGCCTGG GCCAGACCAG TTACAGCAGT TTCAGGTGGT CTGTGAGACT 
GAGGAAGACA AATTCCTCCT GCTGTATGCC CTGCTCAAGC TGTCATTGAT TCGGGGCAAG 
TCTCTGCTCT TTGTCAACAC TCTAGAACGG AGTTACCGGC TACGCCTGTT CTTGGAACAG 
TTCAGCATCC CCACCTGTGT GCTCAATGGA GAGCTTCCAC TGCGCTCCAG GTGCCACATC 
ATCTCACAGT TCAACCAAGG CTTCTACGAC TGTGTCATAG CAACTGATGC TGAAGTCCTG 
GGGGCCCCAG TCAAGGGCAA GCGTCGGGGC CGAGGGCCCA AAGGGGACAA GGCCTCTGAT 
CCGGAAGCAG GTGTGGCCCG GGGCATAGAC TTCCACCATG TGTCTGCTGT GCTCAACTTT 
GATCTTCCCC CAACCCCTGA GGCCTACATC CATCGAGCTG GCAGGACAGC ACGCGCTAAC 
AACCCAGGCA TAGTCTTAAC CTTTGTGCTT CCCACGGAGC AGTTCCACTT AGGCAAGATT 
GAGGAGCTTC TCAGTGGAGA GAACAGGGGC CCCATTCTGC TCCCCTACCA GTTCCGGATG 
GAGGAGATCG AGGGCTTCCG CTATCGCTGC AGGGATGCCA TGCGCTCAGT GACTAAGCAG 
GCCATTCGGG AGGCAAGATT GAAGGAGATC AAGGAAGAGC TTCTGCATTC TGAGAAGCTT 
AAGACATACT TTGAAGACAA CCCTAGGGAC CTCCAGCTGC TGCGGCATGA CCTACCTTTG 
CACCCCGCAG TGGTGAAGCC CCACCTGGGC CATGTTCCTG ACTACCTGGT TCCTCCTGCT 
CTCCGTGGCC TGGTGCGCCC TCACAAGAAG CGGAAGAAGC TGTCTTCCTC TTGTAGGAAG 
GCCAAGAGAG CAAAGTCCCA GAACCCACTG CGCAGCTTCA AGCACAAAGG AAAGAAATTC 
AGACCCACAG CCAAGCCCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061955.1
CDS	644..2287
Translation	MEDSEALGFEHMGLDPRLLQAVTDLGWSRPTLIQEKAIPLALEGKDLLARARTGSGKTAAYAIPMLQLLLHRKATGPVVEQAVRGLVLVPTKELARQAQSMIQQLATYCARDVRVANVSAAEDSVSQRAVLMEKPDVVVGTPSRILSHLQQDSLKLRDSLELLVVDEADLLFSFGFEEELKSLLCHLPRIYQAFLMSATFNEDVQALKELILHNPVTLKLQESQLPGPDQLQQFQVVCETEEDKFLLLYALLKLSLIRGKSLLFVNTLERSYRLRLFLEQFSIPTCVLNGELPLRSRCHIISQFNQGFYDCVIATDAEVLGAPVKGKRRGRGPKGDKASDPEAGVARGIDFHHVSAVLNFDLPPTPEAYIHRAGRTARANNPGIVLTFVLPTEQFHLGKIEELLSGENRGPILLPYQFRMEEIEGFRYRCRDAMRSVTKQAIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPAVVKPHLGHVPDYLVPPALRGLVRPHKKRKKLSSSCRKAKRAKSQNPLRSFKHKGKKFRPTAKPS

Target ORF information:

RefSeq Version	NM_019082.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 56 (DDX56), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019082.3

ORF Insert Sequence:

ATGGAGGACT CTGAAGCACT GGGCTTCGAA CACATGGGCC TCGATCCCCG GCTCCTTCAG 
GCTGTCACCG ATCTGGGCTG GTCGCGACCT ACGCTGATCC AGGAGAAGGC CATCCCACTG 
GCCCTAGAAG GGAAGGACCT CCTGGCTCGG GCCCGCACGG GCTCCGGGAA GACGGCCGCT 
TATGCTATTC CGATGCTGCA GCTGTTGCTC CATAGGAAGG CGACAGGTCC GGTGGTAGAA 
CAGGCAGTGA GAGGCCTTGT TCTTGTTCCT ACCAAGGAGC TGGCACGGCA AGCACAGTCC 
ATGATTCAGC AGCTGGCTAC CTACTGTGCT CGGGATGTCC GAGTGGCCAA TGTCTCAGCT 
GCTGAAGACT CAGTCTCTCA GAGAGCTGTG CTGATGGAGA AGCCAGATGT GGTAGTAGGG 
ACCCCATCTC GCATATTAAG CCACTTGCAG CAAGACAGCC TGAAACTTCG TGACTCCCTG 
GAGCTTTTGG TGGTGGACGA AGCTGACCTT CTTTTTTCCT TTGGCTTTGA AGAAGAGCTC 
AAGAGTCTCC TCTGTCACTT GCCCCGGATT TACCAGGCTT TTCTCATGTC AGCTACTTTT 
AACGAGGACG TACAAGCACT CAAGGAGCTG ATATTACATA ACCCGGTTAC CCTTAAGTTA 
CAGGAGTCCC AGCTGCCTGG GCCAGACCAG TTACAGCAGT TTCAGGTGGT CTGTGAGACT 
GAGGAAGACA AATTCCTCCT GCTGTATGCC CTGCTCAAGC TGTCATTGAT TCGGGGCAAG 
TCTCTGCTCT TTGTCAACAC TCTAGAACGG AGTTACCGGC TACGCCTGTT CTTGGAACAG 
TTCAGCATCC CCACCTGTGT GCTCAATGGA GAGCTTCCAC TGCGCTCCAG GTGCCACATC 
ATCTCACAGT TCAACCAAGG CTTCTACGAC TGTGTCATAG CAACTGATGC TGAAGTCCTG 
GGGGCCCCAG TCAAGGGCAA GCGTCGGGGC CGAGGGCCCA AAGGGGACAA GGCCTCTGAT 
CCGGAAGCAG GTGTGGCCCG GGGCATAGAC TTCCACCATG TGTCTGCTGT GCTCAACTTT 
GATCTTCCCC CAACCCCTGA GGCCTACATC CATCGAGCTG GCAGGACAGC ACGCGCTAAC 
AACCCAGGCA TAGTCTTAAC CTTTGTGCTT CCCACGGAGC AGTTCCACTT AGGCAAGATT 
GAGGAGCTTC TCAGTGGAGA GAACAGGGGC CCCATTCTGC TCCCCTACCA GTTCCGGATG 
GAGGAGATCG AGGGCTTCCG CTATCGCTGC AGGGATGCCA TGCGCTCAGT GACTAAGCAG 
GCCATTCGGG AGGCAAGATT GAAGGAGATC AAGGAAGAGC TTCTGCATTC TGAGAAGCTT 
AAGACATACT TTGAAGACAA CCCTAGGGAC CTCCAGCTGC TGCGGCATGA CCTACCTTTG 
CACCCCGCAG TGGTGAAGCC CCACCTGGGC CATGTTCCTG ACTACCTGGT TCCTCCTGCT 
CTCCGTGGCC TGGTGCGCCC TCACAAGAAG CGGAAGAAGC TGTCTTCCTC TTGTAGGAAG 
GCCAAGAGAG CAAAGTCCCA GAACCCACTG CGCAGCTTCA AGCACAAAGG AAAGAAATTC 
AGACCCACAG CCAAGCCCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX56 ( NM_001257189.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244118.1 Homo sapiens DEAD-box helicase 56 (DDX56), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08291
Clone ID Related Accession (Same CDS sequence)	NM_001257189.1
Accession Version	NM_001257189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX56 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004938.3, AK315363.1 and CA423604.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315363.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGACT CTGAAGCACT GGGCTTCGAA CACATGGGCC TCGATCCCCG GCTCCTTCAG 
GCTGTCACCG ATCTGGGCTG GTCGCGACCT ACGCTGATCC AGGAGAAGGC CATCCCACTG 
GCCCTAGAAG GGAAGGACCT CCTGGCTCGG GCCCGCACGG GCTCCGGGAA GACGGCCGCT 
TATGCTATTC CGATGCTGCA GCTGTTGCTC CATAGGAAGG CGACAGGTCC GGTGGTAGAA 
CAGGCAGTGA GAGGCCTTGT TCTTGTTCCT ACCAAGGAGC TGGCACGGCA AGCACAGTCC 
ATGATTCAGC AGCTGGCTAC CTACTGTGCT CGGGATGTCC GAGTGGCCAA TGTCTCAGCT 
GCTGAAGACT CAGTCTCTCA GAGAGCTGTG CTGATGGAGA AGCCAGATGT GGTAGTAGGG 
ACCCCATCTC GCATATTAAG CCACTTGCAG CAAGACAGCC TGAAACTTCG TGACTCCCTG 
GAGCTTTTGG TGGTGGACGA AGCTGACCTT CTTTTTTCCT TTGGCTTTGA AGAAGAGCTC 
AAGAGTCTCC TCTGTCACTT GCCCCGGATT TACCAGGCTT TTCTCATGTC AGCTACTTTT 
AACGAGGACG TACAAGCACT CAAGGAGCTG ATATTACATA ACCCGGTTAC CCTTAAGTTA 
CAGGAGTCCC AGCTGCCTGG GCCAGACCAG TTACAGCAGT TTCAGGTGGT CTGTGAGACT 
GAGGAAGACA AATTCCTCCT GCTGTATGCC CTGCTCAAGC TGTCATTGAT TCGGGGCAAG 
TCTCTGCTCT TTGTCAACAC TCTAGAACGG AGTTACCGGC TACGCCTGTT CTTGGAACAG 
TTCAGCATCC CCACCTGTGT GCTCAATGGA GAGCTTCCAC TGCGCTCCAG GGCCTCTGAT 
CCGGAAGCAG GTGTGGCCCG GGGCATAGAC TTCCACCATG TGTCTGCTGT GCTCAACTTT 
GATCTTCCCC CAACCCCTGA GGCCTACATC CATCGAGCTG GCAGGACAGC ACGCGCTAAC 
AACCCAGGCA TAGTCTTAAC CTTTGTGCTT CCCACGGAGC AGTTCCACTT AGGCAAGATT 
GAGGAGCTTC TCAGTGGAGA GAACAGGGGC CCCATTCTGC TCCCCTACCA GTTCCGGATG 
GAGGAGATCG AGGGCTTCCG CTATCGCTGC AGGGATGCCA TGCGCTCAGT GACTAAGCAG 
GCCATTCGGG AGGCAAGATT GAAGGAGATC AAGGAAGAGC TTCTGCATTC TGAGAAGCTT 
AAGACATACT TTGAAGACAA CCCTAGGGAC CTCCAGCTGC TGCGGCATGA CCTACCTTTG 
CACCCCGCAG TGGTGAAGCC CCACCTGGGC CATGTTCCTG ACTACCTGGT TCCTCCTGCT 
CTCCGTGGCC TGGTGCGCCC TCACAAGAAG CGGAAGAAGC TGTCTTCCTC TTGTAGGAAG 
GCCAAGAGAG CAAAGTCCCA GAACCCACTG CGCAGCTTCA AGCACAAAGG AAAGAAATTC 
AGACCCACAG CCAAGCCCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244118.1
CDS	644..2167
Translation	MEDSEALGFEHMGLDPRLLQAVTDLGWSRPTLIQEKAIPLALEGKDLLARARTGSGKTAAYAIPMLQLLLHRKATGPVVEQAVRGLVLVPTKELARQAQSMIQQLATYCARDVRVANVSAAEDSVSQRAVLMEKPDVVVGTPSRILSHLQQDSLKLRDSLELLVVDEADLLFSFGFEEELKSLLCHLPRIYQAFLMSATFNEDVQALKELILHNPVTLKLQESQLPGPDQLQQFQVVCETEEDKFLLLYALLKLSLIRGKSLLFVNTLERSYRLRLFLEQFSIPTCVLNGELPLRSRASDPEAGVARGIDFHHVSAVLNFDLPPTPEAYIHRAGRTARANNPGIVLTFVLPTEQFHLGKIEELLSGENRGPILLPYQFRMEEIEGFRYRCRDAMRSVTKQAIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPAVVKPHLGHVPDYLVPPALRGLVRPHKKRKKLSSSCRKAKRAKSQNPLRSFKHKGKKFRPTAKPS

Target ORF information:

RefSeq Version	NM_001257189.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 56 (DDX56), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001257189.1

ORF Insert Sequence:

ATGGAGGACT CTGAAGCACT GGGCTTCGAA CACATGGGCC TCGATCCCCG GCTCCTTCAG 
GCTGTCACCG ATCTGGGCTG GTCGCGACCT ACGCTGATCC AGGAGAAGGC CATCCCACTG 
GCCCTAGAAG GGAAGGACCT CCTGGCTCGG GCCCGCACGG GCTCCGGGAA GACGGCCGCT 
TATGCTATTC CGATGCTGCA GCTGTTGCTC CATAGGAAGG CGACAGGTCC GGTGGTAGAA 
CAGGCAGTGA GAGGCCTTGT TCTTGTTCCT ACCAAGGAGC TGGCACGGCA AGCACAGTCC 
ATGATTCAGC AGCTGGCTAC CTACTGTGCT CGGGATGTCC GAGTGGCCAA TGTCTCAGCT 
GCTGAAGACT CAGTCTCTCA GAGAGCTGTG CTGATGGAGA AGCCAGATGT GGTAGTAGGG 
ACCCCATCTC GCATATTAAG CCACTTGCAG CAAGACAGCC TGAAACTTCG TGACTCCCTG 
GAGCTTTTGG TGGTGGACGA AGCTGACCTT CTTTTTTCCT TTGGCTTTGA AGAAGAGCTC 
AAGAGTCTCC TCTGTCACTT GCCCCGGATT TACCAGGCTT TTCTCATGTC AGCTACTTTT 
AACGAGGACG TACAAGCACT CAAGGAGCTG ATATTACATA ACCCGGTTAC CCTTAAGTTA 
CAGGAGTCCC AGCTGCCTGG GCCAGACCAG TTACAGCAGT TTCAGGTGGT CTGTGAGACT 
GAGGAAGACA AATTCCTCCT GCTGTATGCC CTGCTCAAGC TGTCATTGAT TCGGGGCAAG 
TCTCTGCTCT TTGTCAACAC TCTAGAACGG AGTTACCGGC TACGCCTGTT CTTGGAACAG 
TTCAGCATCC CCACCTGTGT GCTCAATGGA GAGCTTCCAC TGCGCTCCAG GGCCTCTGAT 
CCGGAAGCAG GTGTGGCCCG GGGCATAGAC TTCCACCATG TGTCTGCTGT GCTCAACTTT 
GATCTTCCCC CAACCCCTGA GGCCTACATC CATCGAGCTG GCAGGACAGC ACGCGCTAAC 
AACCCAGGCA TAGTCTTAAC CTTTGTGCTT CCCACGGAGC AGTTCCACTT AGGCAAGATT 
GAGGAGCTTC TCAGTGGAGA GAACAGGGGC CCCATTCTGC TCCCCTACCA GTTCCGGATG 
GAGGAGATCG AGGGCTTCCG CTATCGCTGC AGGGATGCCA TGCGCTCAGT GACTAAGCAG 
GCCATTCGGG AGGCAAGATT GAAGGAGATC AAGGAAGAGC TTCTGCATTC TGAGAAGCTT 
AAGACATACT TTGAAGACAA CCCTAGGGAC CTCCAGCTGC TGCGGCATGA CCTACCTTTG 
CACCCCGCAG TGGTGAAGCC CCACCTGGGC CATGTTCCTG ACTACCTGGT TCCTCCTGCT 
CTCCGTGGCC TGGTGCGCCC TCACAAGAAG CGGAAGAAGC TGTCTTCCTC TTGTAGGAAG 
GCCAAGAGAG CAAAGTCCCA GAACCCACTG CGCAGCTTCA AGCACAAAGG AAAGAAATTC 
AGACCCACAG CCAAGCCCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleolar helicase DDX56 redistributes to West Nile virus assembly sites.
Virology500169-177(2017 01)
Reid CR,Hobman TC

The helicase activity of DDX56 is required for its role in assembly of infectious West Nile virus particles.
Virology433(1)226-35(2012 Nov)
Xu Z,Hobman TC

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

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DDX56 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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