RNF216 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_207111.3	NP_996994.1	E3 ubiquitin-protein ligase RNF216 isoform a
XM_005249785.2	XP_005249842.1	E3 ubiquitin-protein ligase RNF216 isoform X1
NM_207111.4	NP_996994.1	E3 ubiquitin-protein ligase RNF216 isoform a
XM_017012363.1	XP_016867852.1	E3 ubiquitin-protein ligase RNF216 isoform X2
XM_017012365.1	XP_016867854.1	E3 ubiquitin-protein ligase RNF216 isoform X4
XM_011515436.1	XP_011513738.1	E3 ubiquitin-protein ligase RNF216 isoform X4
XM_017012363.2	XP_016867852.1	E3 ubiquitin-protein ligase RNF216 isoform X2
XM_024446805.1	XP_024302573.1	E3 ubiquitin-protein ligase RNF216 isoform X1
XM_024446806.1	XP_024302574.1	E3 ubiquitin-protein ligase RNF216 isoform X4
XM_011515436.2	XP_011513738.1	E3 ubiquitin-protein ligase RNF216 isoform X4
XM_017012364.1	XP_016867853.1	E3 ubiquitin-protein ligase RNF216 isoform X3
XM_017012364.2	XP_016867853.1	E3 ubiquitin-protein ligase RNF216 isoform X3
XM_024446807.1	XP_024302575.1	E3 ubiquitin-protein ligase RNF216 isoform X4
NM_207116.3	NP_996999.1	E3 ubiquitin-protein ligase RNF216 isoform b
NM_207116.2	NP_996999.1	E3 ubiquitin-protein ligase RNF216 isoform b
NM_001377156.1	NP_001364085.1	E3 ubiquitin-protein ligase RNF216 isoform b

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08423	NM_001377156.1 Latest version!	Homo sapiens ring finger protein 216 (RNF216), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu08423	NM_207116.3 Latest version!	Homo sapiens ring finger protein 216 (RNF216), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu22477	NM_207111.4 Latest version!	Homo sapiens ring finger protein 216 (RNF216), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu08423	XM_017012363.2 Latest version!	Homo sapiens ring finger protein 216 (RNF216), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu41206	XM_017012365.1 Latest version!	Homo sapiens ring finger protein 216 (RNF216), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu41206	XM_024446807.1 Latest version!	Homo sapiens ring finger protein 216 (RNF216), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu41206	XM_024446806.1 Latest version!	Homo sapiens ring finger protein 216 (RNF216), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu41206	XM_011515436.2 Latest version!	Homo sapiens ring finger protein 216 (RNF216), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu22477	XM_024446805.1 Latest version!	Homo sapiens ring finger protein 216 (RNF216), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu22477	XM_005249785.2 Latest version!	Homo sapiens ring finger protein 216 (RNF216), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu97361	XM_017012364.2 Latest version!	Homo sapiens ring finger protein 216 (RNF216), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu22477	NM_207111.3	Homo sapiens ring finger protein 216 (RNF216), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu08423	XM_017012363.1	Homo sapiens ring finger protein 216 (RNF216), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu41206	XM_011515436.1	Homo sapiens ring finger protein 216 (RNF216), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu97361	XM_017012364.1	Homo sapiens ring finger protein 216 (RNF216), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu08423	NM_207116.2	Homo sapiens ring finger protein 216 (RNF216), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

RNF216 ( NM_207116.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_996999.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08423
Clone ID Related Accession (Same CDS sequence)	XM_017012363.1 , XM_017012363.2 , NM_207116.3 , NM_207116.2 , NM_001377156.1
Accession Version	NM_207116.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008167.5 and AC006483.3. On Jun 2, 2019 this sequence version replaced NM_207116.2. Transcript Variant: This variant (2, also known as TRIAD3A) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform b which is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.162394.1, SRR1660809.253601.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AGATGATTCT GAGGATGACT ACGGTGAATT TCTGGATCTT 
GGGCCTCCTG GAATCTCTGA ATTCACTAAG CCAAGTGGCC AAACAGAAAG AGAACCCAAG 
CCTGGACCGA GTCATAACCA AGCAGCAAAT GACATTGTCA ACCCCAGATC AGAGCAGAAA 
GTCATCATCT TGGAAGAAGG TAGCCTTCTT TACACAGAAA GCGATCCTTT GGAAACTCAG 
AACCAGTCAT CCGAAGACTC AGAGACAGAG CTGTTATCAA ATCTAGGAGA GTCAGCTGCT 
CTAGCAGATG ATCAGGCCAT CGAAGAAGAC TGCTGGTTAG ATCATCCTTA CTTCCAGTCT 
CTGAACCAAC AGCCCCGTGA AATAACAAAC CAGGTCGTTC CTCAGGAACG GCAGCCTGAA 
GCAGAACTGG GCCGCTTGTT GTTTCAGCAT GAATTCCCAG GGCCCGCTTT TCCAAGGCCG 
GAACCCCAGC AAGGTGGGAT TTCAGGCCCC TCTTCTCCTC AGCCTGCCCA TCCTCTAGGA 
GAGTTTGAAG ACCAGCAGTT AGCAAGTGAT GATGAAGAGC CAGGTCCAGC CTTTCCAATG 
CAAGAATCTC AAGAGCCCAA TTTGGAAAAC ATTTGGGGGC AAGAAGCTGC AGAGGTAGAT 
CAAGAGCTCG TTGAACTACT AGTGAAAGAA ACGGAAGCAA GATTTCCAGA TGTAGCAAAT 
GGGTTTATTG AGGAAATAAT TCATTTTAAG AATTATTATG ATCTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAACCCAGA TTATCCAAAG AGAGAAGACA GAATCATTAT AAATCCCAGT 
AGCAGTCTGC TGGCCAGCCA AGATGAGACA AAGTTGCCTA AAATAGACTT TTTTGACTAT 
TCTAAATTGA CCCCTCTTGA CCAGCGCTGC TTCATCCAAG CTGCTGACCT CCTCATGGCC 
GACTTCAAAG TGCTCAGTAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTGTCTGAT GCCATTAAAA AATGGCAGGA GCTGTCACCA 
GAAACCAGTG GAAAAAGGAA GAAGAGAAAA CAAATGAACC AGTATTCTTA CATTGATTTC 
AAGTTTGAAC AAGGTGACAT AAAAATAGAA AAGAGGATGT TCTTTCTTGA AAATAAGCGA 
CGACATTGTA GGTCCTATGA CCGACGTGCT CTCCTTCCAG CTGTGCAACA AGAGCAGGAG 
TTCTATGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTACAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGTCGCTG CTGCTATGGG 
GAATTTCCAT TCGAGGAGCT GACGCAGTGC GCAGATGCTC ACTTGTTCTG CAAAGAGTGT 
CTCATCAGAT ATGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTTGGAGCT CAGCTGCATG 
GAAGGCAGCT GCACGTGTTC GTTCCCAACC AGTGAGCTGG AGAAGGTGCT CCCCCAGACC 
ATCCTGTATA AGTACTATGA GCGAAAAGCC GAGGAGGAGG TTGCGGCAGC CTACGCCGAC 
GAGCTTGTCA GGTGCCCGTC CTGTAGCTTT CCGGCTCTGT TGGACAGTGA TGTGAAGAGG 
TTCAGCTGTC CTAATCCTCA CTGCCGAAAG GAAACCTGTA GGAAGTGTCA GGGACTCTGG 
AAAGAACATA ATGGCCTCAC CTGTGAAGAG CTGGCTGAAA AAGACGACAT CAAGTACCGT 
ACCTCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGAA AATGCCACAA GTGTGGGACT 
GGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCTTGCC GCTGTGGTGC CCAGATGTGC 
TACCTCTGTC GAGTTTCTAT TAATGGATAT GACCATTTCT GCCAACATCC CCGCTCACCA 
GGAGCCCCTT GCCAGGAGTG TTCAAGATGC TCTCTCTGGA CCGATCCCAC TGAAGATGAT 
GAGAAGCTTA TTGAGGAAAT CCAGAAGGAG GCTGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT TGGACCCCCG CTGGAGAAGC CTGTGGAGAA GGTGCAGAGG 
GTGGAGGCCC TCCCGAGGCC CGTTCCGCAG AACCTGCCAC AGCCACAGAT GCCACCCTAT 
GCCTTCGCGC ACCCACCCTT CCCCCTGCCT CCCGTGCGGC CTGTGTTCAA CAACTTCCCA 
CTCAACATGG GGCCTATCCC AGCCCCGTAC GTGCCCCCTC TGCCCAACGT GCGGGTCAAC 
TATGACTTCG GTCCCATCCA CATGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCGCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996999.1
CDS	193..2793
Translation	MEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIPMLVTPAPQQHEEEDLDDDVILTEDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGPSHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAALADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPRPEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAAEVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRIIINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	NM_207116.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207116.3

ORF Insert Sequence:

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AGATGATTCT GAGGATGACT ACGGTGAATT TCTGGATCTT 
GGGCCTCCTG GAATCTCTGA ATTCACTAAG CCAAGTGGCC AAACAGAAAG AGAACCCAAG 
CCTGGACCGA GTCATAACCA AGCAGCAAAT GACATTGTCA ACCCCAGATC AGAGCAGAAA 
GTCATCATCT TGGAAGAAGG TAGCCTTCTT TACACAGAAA GCGATCCTTT GGAAACTCAG 
AACCAGTCAT CCGAAGACTC AGAGACAGAG CTGTTATCAA ATCTAGGAGA GTCAGCTGCT 
CTAGCAGATG ATCAGGCCAT CGAAGAAGAC TGCTGGTTAG ATCATCCTTA CTTCCAGTCT 
CTGAACCAAC AGCCCCGTGA AATAACAAAC CAGGTCGTTC CTCAGGAACG GCAGCCTGAA 
GCAGAACTGG GCCGCTTGTT GTTTCAGCAT GAATTCCCAG GGCCCGCTTT TCCAAGGCCG 
GAACCCCAGC AAGGTGGGAT TTCAGGCCCC TCTTCTCCTC AGCCTGCCCA TCCTCTAGGA 
GAGTTTGAAG ACCAGCAGTT AGCAAGTGAT GATGAAGAGC CAGGTCCAGC CTTTCCAATG 
CAAGAATCTC AAGAGCCCAA TTTGGAAAAC ATTTGGGGGC AAGAAGCTGC AGAGGTAGAT 
CAAGAGCTCG TTGAACTACT AGTGAAAGAA ACGGAAGCAA GATTTCCAGA TGTAGCAAAT 
GGGTTTATTG AGGAAATAAT TCATTTTAAG AATTATTATG ATCTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAACCCAGA TTATCCAAAG AGAGAAGACA GAATCATTAT AAATCCCAGT 
AGCAGTCTGC TGGCCAGCCA AGATGAGACA AAGTTGCCTA AAATAGACTT TTTTGACTAT 
TCTAAATTGA CCCCTCTTGA CCAGCGCTGC TTCATCCAAG CTGCTGACCT CCTCATGGCC 
GACTTCAAAG TGCTCAGTAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTGTCTGAT GCCATTAAAA AATGGCAGGA GCTGTCACCA 
GAAACCAGTG GAAAAAGGAA GAAGAGAAAA CAAATGAACC AGTATTCTTA CATTGATTTC 
AAGTTTGAAC AAGGTGACAT AAAAATAGAA AAGAGGATGT TCTTTCTTGA AAATAAGCGA 
CGACATTGTA GGTCCTATGA CCGACGTGCT CTCCTTCCAG CTGTGCAACA AGAGCAGGAG 
TTCTATGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTACAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGTCGCTG CTGCTATGGG 
GAATTTCCAT TCGAGGAGCT GACGCAGTGC GCAGATGCTC ACTTGTTCTG CAAAGAGTGT 
CTCATCAGAT ATGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTTGGAGCT CAGCTGCATG 
GAAGGCAGCT GCACGTGTTC GTTCCCAACC AGTGAGCTGG AGAAGGTGCT CCCCCAGACC 
ATCCTGTATA AGTACTATGA GCGAAAAGCC GAGGAGGAGG TTGCGGCAGC CTACGCCGAC 
GAGCTTGTCA GGTGCCCGTC CTGTAGCTTT CCGGCTCTGT TGGACAGTGA TGTGAAGAGG 
TTCAGCTGTC CTAATCCTCA CTGCCGAAAG GAAACCTGTA GGAAGTGTCA GGGACTCTGG 
AAAGAACATA ATGGCCTCAC CTGTGAAGAG CTGGCTGAAA AAGACGACAT CAAGTACCGT 
ACCTCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGAA AATGCCACAA GTGTGGGACT 
GGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCTTGCC GCTGTGGTGC CCAGATGTGC 
TACCTCTGTC GAGTTTCTAT TAATGGATAT GACCATTTCT GCCAACATCC CCGCTCACCA 
GGAGCCCCTT GCCAGGAGTG TTCAAGATGC TCTCTCTGGA CCGATCCCAC TGAAGATGAT 
GAGAAGCTTA TTGAGGAAAT CCAGAAGGAG GCTGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT TGGACCCCCG CTGGAGAAGC CTGTGGAGAA GGTGCAGAGG 
GTGGAGGCCC TCCCGAGGCC CGTTCCGCAG AACCTGCCAC AGCCACAGAT GCCACCCTAT 
GCCTTCGCGC ACCCACCCTT CCCCCTGCCT CCCGTGCGGC CTGTGTTCAA CAACTTCCCA 
CTCAACATGG GGCCTATCCC AGCCCCGTAC GTGCCCCCTC TGCCCAACGT GCGGGTCAAC 
TATGACTTCG GTCCCATCCA CATGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCGCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( NM_207116.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_996999.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08423
Clone ID Related Accession (Same CDS sequence)	XM_017012363.1 , XM_017012363.2 , NM_207116.3 , NM_207116.2 , NM_001377156.1
Accession Version	NM_207116.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292396.1, AF513717.1, AC006483.3 and BM970811.1. On Jan 21, 2011 this sequence version replaced NM_207116.1. Transcript Variant: This variant (2, also known as TRIAD3A) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform b which is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.142816.1, SRR1660805.106937.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AGATGATTCT GAGGATGACT ACGGTGAATT TCTGGATCTT 
GGGCCTCCTG GAATCTCTGA ATTCACTAAG CCAAGTGGCC AAACAGAAAG AGAACCCAAG 
CCTGGACCGA GTCATAACCA AGCAGCAAAT GACATTGTCA ACCCCAGATC AGAGCAGAAA 
GTCATCATCT TGGAAGAAGG TAGCCTTCTT TACACAGAAA GCGATCCTTT GGAAACTCAG 
AACCAGTCAT CCGAAGACTC AGAGACAGAG CTGTTATCAA ATCTAGGAGA GTCAGCTGCT 
CTAGCAGATG ATCAGGCCAT CGAAGAAGAC TGCTGGTTAG ATCATCCTTA CTTCCAGTCT 
CTGAACCAAC AGCCCCGTGA AATAACAAAC CAGGTCGTTC CTCAGGAACG GCAGCCTGAA 
GCAGAACTGG GCCGCTTGTT GTTTCAGCAT GAATTCCCAG GGCCCGCTTT TCCAAGGCCG 
GAACCCCAGC AAGGTGGGAT TTCAGGCCCC TCTTCTCCTC AGCCTGCCCA TCCTCTAGGA 
GAGTTTGAAG ACCAGCAGTT AGCAAGTGAT GATGAAGAGC CAGGTCCAGC CTTTCCAATG 
CAAGAATCTC AAGAGCCCAA TTTGGAAAAC ATTTGGGGGC AAGAAGCTGC AGAGGTAGAT 
CAAGAGCTCG TTGAACTACT AGTGAAAGAA ACGGAAGCAA GATTTCCAGA TGTAGCAAAT 
GGGTTTATTG AGGAAATAAT TCATTTTAAG AATTATTATG ATCTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAACCCAGA TTATCCAAAG AGAGAAGACA GAATCATTAT AAATCCCAGT 
AGCAGTCTGC TGGCCAGCCA AGATGAGACA AAGTTGCCTA AAATAGACTT TTTTGACTAT 
TCTAAATTGA CCCCTCTTGA CCAGCGCTGC TTCATCCAAG CTGCTGACCT CCTCATGGCC 
GACTTCAAAG TGCTCAGTAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTGTCTGAT GCCATTAAAA AATGGCAGGA GCTGTCACCA 
GAAACCAGTG GAAAAAGGAA GAAGAGAAAA CAAATGAACC AGTATTCTTA CATTGATTTC 
AAGTTTGAAC AAGGTGACAT AAAAATAGAA AAGAGGATGT TCTTTCTTGA AAATAAGCGA 
CGACATTGTA GGTCCTATGA CCGACGTGCT CTCCTTCCAG CTGTGCAACA AGAGCAGGAG 
TTCTATGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTACAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGTCGCTG CTGCTATGGG 
GAATTTCCAT TCGAGGAGCT GACGCAGTGC GCAGATGCTC ACTTGTTCTG CAAAGAGTGT 
CTCATCAGAT ATGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTTGGAGCT CAGCTGCATG 
GAAGGCAGCT GCACGTGTTC GTTCCCAACC AGTGAGCTGG AGAAGGTGCT CCCCCAGACC 
ATCCTGTATA AGTACTATGA GCGAAAAGCC GAGGAGGAGG TTGCGGCAGC CTACGCCGAC 
GAGCTTGTCA GGTGCCCGTC CTGTAGCTTT CCGGCTCTGT TGGACAGTGA TGTGAAGAGG 
TTCAGCTGTC CTAATCCTCA CTGCCGAAAG GAAACCTGTA GGAAGTGTCA GGGACTCTGG 
AAAGAACATA ATGGCCTCAC CTGTGAAGAG CTGGCTGAAA AAGACGACAT CAAGTACCGT 
ACCTCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGAA AATGCCACAA GTGTGGGACT 
GGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCTTGCC GCTGTGGTGC CCAGATGTGC 
TACCTCTGTC GAGTTTCTAT TAATGGATAT GACCATTTCT GCCAACATCC CCGCTCACCA 
GGAGCCCCTT GCCAGGAGTG TTCAAGATGC TCTCTCTGGA CCGATCCCAC TGAAGATGAT 
GAGAAGCTTA TTGAGGAAAT CCAGAAGGAG GCTGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT TGGACCCCCG CTGGAGAAGC CTGTGGAGAA GGTGCAGAGG 
GTGGAGGCCC TCCCGAGGCC CGTTCCGCAG AACCTGCCAC AGCCACAGAT GCCACCCTAT 
GCCTTCGCGC ACCCACCCTT CCCCCTGCCT CCCGTGCGGC CTGTGTTCAA CAACTTCCCA 
CTCAACATGG GGCCTATCCC AGCCCCGTAC GTGCCCCCTC TGCCCAACGT GCGGGTCAAC 
TATGACTTCG GTCCCATCCA CATGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCGCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996999.1
CDS	260..2860
Translation	MEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIPMLVTPAPQQHEEEDLDDDVILTEDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGPSHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAALADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPRPEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAAEVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRIIINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	NM_207116.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207116.2

ORF Insert Sequence:

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AGATGATTCT GAGGATGACT ACGGTGAATT TCTGGATCTT 
GGGCCTCCTG GAATCTCTGA ATTCACTAAG CCAAGTGGCC AAACAGAAAG AGAACCCAAG 
CCTGGACCGA GTCATAACCA AGCAGCAAAT GACATTGTCA ACCCCAGATC AGAGCAGAAA 
GTCATCATCT TGGAAGAAGG TAGCCTTCTT TACACAGAAA GCGATCCTTT GGAAACTCAG 
AACCAGTCAT CCGAAGACTC AGAGACAGAG CTGTTATCAA ATCTAGGAGA GTCAGCTGCT 
CTAGCAGATG ATCAGGCCAT CGAAGAAGAC TGCTGGTTAG ATCATCCTTA CTTCCAGTCT 
CTGAACCAAC AGCCCCGTGA AATAACAAAC CAGGTCGTTC CTCAGGAACG GCAGCCTGAA 
GCAGAACTGG GCCGCTTGTT GTTTCAGCAT GAATTCCCAG GGCCCGCTTT TCCAAGGCCG 
GAACCCCAGC AAGGTGGGAT TTCAGGCCCC TCTTCTCCTC AGCCTGCCCA TCCTCTAGGA 
GAGTTTGAAG ACCAGCAGTT AGCAAGTGAT GATGAAGAGC CAGGTCCAGC CTTTCCAATG 
CAAGAATCTC AAGAGCCCAA TTTGGAAAAC ATTTGGGGGC AAGAAGCTGC AGAGGTAGAT 
CAAGAGCTCG TTGAACTACT AGTGAAAGAA ACGGAAGCAA GATTTCCAGA TGTAGCAAAT 
GGGTTTATTG AGGAAATAAT TCATTTTAAG AATTATTATG ATCTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAACCCAGA TTATCCAAAG AGAGAAGACA GAATCATTAT AAATCCCAGT 
AGCAGTCTGC TGGCCAGCCA AGATGAGACA AAGTTGCCTA AAATAGACTT TTTTGACTAT 
TCTAAATTGA CCCCTCTTGA CCAGCGCTGC TTCATCCAAG CTGCTGACCT CCTCATGGCC 
GACTTCAAAG TGCTCAGTAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTGTCTGAT GCCATTAAAA AATGGCAGGA GCTGTCACCA 
GAAACCAGTG GAAAAAGGAA GAAGAGAAAA CAAATGAACC AGTATTCTTA CATTGATTTC 
AAGTTTGAAC AAGGTGACAT AAAAATAGAA AAGAGGATGT TCTTTCTTGA AAATAAGCGA 
CGACATTGTA GGTCCTATGA CCGACGTGCT CTCCTTCCAG CTGTGCAACA AGAGCAGGAG 
TTCTATGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTACAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGTCGCTG CTGCTATGGG 
GAATTTCCAT TCGAGGAGCT GACGCAGTGC GCAGATGCTC ACTTGTTCTG CAAAGAGTGT 
CTCATCAGAT ATGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTTGGAGCT CAGCTGCATG 
GAAGGCAGCT GCACGTGTTC GTTCCCAACC AGTGAGCTGG AGAAGGTGCT CCCCCAGACC 
ATCCTGTATA AGTACTATGA GCGAAAAGCC GAGGAGGAGG TTGCGGCAGC CTACGCCGAC 
GAGCTTGTCA GGTGCCCGTC CTGTAGCTTT CCGGCTCTGT TGGACAGTGA TGTGAAGAGG 
TTCAGCTGTC CTAATCCTCA CTGCCGAAAG GAAACCTGTA GGAAGTGTCA GGGACTCTGG 
AAAGAACATA ATGGCCTCAC CTGTGAAGAG CTGGCTGAAA AAGACGACAT CAAGTACCGT 
ACCTCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGAA AATGCCACAA GTGTGGGACT 
GGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCTTGCC GCTGTGGTGC CCAGATGTGC 
TACCTCTGTC GAGTTTCTAT TAATGGATAT GACCATTTCT GCCAACATCC CCGCTCACCA 
GGAGCCCCTT GCCAGGAGTG TTCAAGATGC TCTCTCTGGA CCGATCCCAC TGAAGATGAT 
GAGAAGCTTA TTGAGGAAAT CCAGAAGGAG GCTGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT TGGACCCCCG CTGGAGAAGC CTGTGGAGAA GGTGCAGAGG 
GTGGAGGCCC TCCCGAGGCC CGTTCCGCAG AACCTGCCAC AGCCACAGAT GCCACCCTAT 
GCCTTCGCGC ACCCACCCTT CCCCCTGCCT CCCGTGCGGC CTGTGTTCAA CAACTTCCCA 
CTCAACATGG GGCCTATCCC AGCCCCGTAC GTGCCCCCTC TGCCCAACGT GCGGGTCAAC 
TATGACTTCG GTCCCATCCA CATGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCGCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( NM_001377156.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001364085.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu08423
Clone ID Related Accession (Same CDS sequence)	XM_017012363.1 , XM_017012363.2 , NM_207116.3 , NM_207116.2 , NM_001377156.1
Accession Version	NM_001377156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008167.5 and AC006483.3. On Dec 17, 2019 this sequence version replaced XM_017012363.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.80193.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AGATGATTCT GAGGATGACT ACGGTGAATT TCTGGATCTT 
GGGCCTCCTG GAATCTCTGA ATTCACTAAG CCAAGTGGCC AAACAGAAAG AGAACCCAAG 
CCTGGACCGA GTCATAACCA AGCAGCAAAT GACATTGTCA ACCCCAGATC AGAGCAGAAA 
GTCATCATCT TGGAAGAAGG TAGCCTTCTT TACACAGAAA GCGATCCTTT GGAAACTCAG 
AACCAGTCAT CCGAAGACTC AGAGACAGAG CTGTTATCAA ATCTAGGAGA GTCAGCTGCT 
CTAGCAGATG ATCAGGCCAT CGAAGAAGAC TGCTGGTTAG ATCATCCTTA CTTCCAGTCT 
CTGAACCAAC AGCCCCGTGA AATAACAAAC CAGGTCGTTC CTCAGGAACG GCAGCCTGAA 
GCAGAACTGG GCCGCTTGTT GTTTCAGCAT GAATTCCCAG GGCCCGCTTT TCCAAGGCCG 
GAACCCCAGC AAGGTGGGAT TTCAGGCCCC TCTTCTCCTC AGCCTGCCCA TCCTCTAGGA 
GAGTTTGAAG ACCAGCAGTT AGCAAGTGAT GATGAAGAGC CAGGTCCAGC CTTTCCAATG 
CAAGAATCTC AAGAGCCCAA TTTGGAAAAC ATTTGGGGGC AAGAAGCTGC AGAGGTAGAT 
CAAGAGCTCG TTGAACTACT AGTGAAAGAA ACGGAAGCAA GATTTCCAGA TGTAGCAAAT 
GGGTTTATTG AGGAAATAAT TCATTTTAAG AATTATTATG ATCTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAACCCAGA TTATCCAAAG AGAGAAGACA GAATCATTAT AAATCCCAGT 
AGCAGTCTGC TGGCCAGCCA AGATGAGACA AAGTTGCCTA AAATAGACTT TTTTGACTAT 
TCTAAATTGA CCCCTCTTGA CCAGCGCTGC TTCATCCAAG CTGCTGACCT CCTCATGGCC 
GACTTCAAAG TGCTCAGTAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTGTCTGAT GCCATTAAAA AATGGCAGGA GCTGTCACCA 
GAAACCAGTG GAAAAAGGAA GAAGAGAAAA CAAATGAACC AGTATTCTTA CATTGATTTC 
AAGTTTGAAC AAGGTGACAT AAAAATAGAA AAGAGGATGT TCTTTCTTGA AAATAAGCGA 
CGACATTGTA GGTCCTATGA CCGACGTGCT CTCCTTCCAG CTGTGCAACA AGAGCAGGAG 
TTCTATGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTACAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGTCGCTG CTGCTATGGG 
GAATTTCCAT TCGAGGAGCT GACGCAGTGC GCAGATGCTC ACTTGTTCTG CAAAGAGTGT 
CTCATCAGAT ATGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTTGGAGCT CAGCTGCATG 
GAAGGCAGCT GCACGTGTTC GTTCCCAACC AGTGAGCTGG AGAAGGTGCT CCCCCAGACC 
ATCCTGTATA AGTACTATGA GCGAAAAGCC GAGGAGGAGG TTGCGGCAGC CTACGCCGAC 
GAGCTTGTCA GGTGCCCGTC CTGTAGCTTT CCGGCTCTGT TGGACAGTGA TGTGAAGAGG 
TTCAGCTGTC CTAATCCTCA CTGCCGAAAG GAAACCTGTA GGAAGTGTCA GGGACTCTGG 
AAAGAACATA ATGGCCTCAC CTGTGAAGAG CTGGCTGAAA AAGACGACAT CAAGTACCGT 
ACCTCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGAA AATGCCACAA GTGTGGGACT 
GGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCTTGCC GCTGTGGTGC CCAGATGTGC 
TACCTCTGTC GAGTTTCTAT TAATGGATAT GACCATTTCT GCCAACATCC CCGCTCACCA 
GGAGCCCCTT GCCAGGAGTG TTCAAGATGC TCTCTCTGGA CCGATCCCAC TGAAGATGAT 
GAGAAGCTTA TTGAGGAAAT CCAGAAGGAG GCTGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT TGGACCCCCG CTGGAGAAGC CTGTGGAGAA GGTGCAGAGG 
GTGGAGGCCC TCCCGAGGCC CGTTCCGCAG AACCTGCCAC AGCCACAGAT GCCACCCTAT 
GCCTTCGCGC ACCCACCCTT CCCCCTGCCT CCCGTGCGGC CTGTGTTCAA CAACTTCCCA 
CTCAACATGG GGCCTATCCC AGCCCCGTAC GTGCCCCCTC TGCCCAACGT GCGGGTCAAC 
TATGACTTCG GTCCCATCCA CATGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCGCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001364085.1
CDS	238..2838
Translation	MEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIPMLVTPAPQQHEEEDLDDDVILTEDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGPSHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAALADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPRPEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAAEVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRIIINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	NM_001377156.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377156.1

ORF Insert Sequence:

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AGATGATTCT GAGGATGACT ACGGTGAATT TCTGGATCTT 
GGGCCTCCTG GAATCTCTGA ATTCACTAAG CCAAGTGGCC AAACAGAAAG AGAACCCAAG 
CCTGGACCGA GTCATAACCA AGCAGCAAAT GACATTGTCA ACCCCAGATC AGAGCAGAAA 
GTCATCATCT TGGAAGAAGG TAGCCTTCTT TACACAGAAA GCGATCCTTT GGAAACTCAG 
AACCAGTCAT CCGAAGACTC AGAGACAGAG CTGTTATCAA ATCTAGGAGA GTCAGCTGCT 
CTAGCAGATG ATCAGGCCAT CGAAGAAGAC TGCTGGTTAG ATCATCCTTA CTTCCAGTCT 
CTGAACCAAC AGCCCCGTGA AATAACAAAC CAGGTCGTTC CTCAGGAACG GCAGCCTGAA 
GCAGAACTGG GCCGCTTGTT GTTTCAGCAT GAATTCCCAG GGCCCGCTTT TCCAAGGCCG 
GAACCCCAGC AAGGTGGGAT TTCAGGCCCC TCTTCTCCTC AGCCTGCCCA TCCTCTAGGA 
GAGTTTGAAG ACCAGCAGTT AGCAAGTGAT GATGAAGAGC CAGGTCCAGC CTTTCCAATG 
CAAGAATCTC AAGAGCCCAA TTTGGAAAAC ATTTGGGGGC AAGAAGCTGC AGAGGTAGAT 
CAAGAGCTCG TTGAACTACT AGTGAAAGAA ACGGAAGCAA GATTTCCAGA TGTAGCAAAT 
GGGTTTATTG AGGAAATAAT TCATTTTAAG AATTATTATG ATCTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAACCCAGA TTATCCAAAG AGAGAAGACA GAATCATTAT AAATCCCAGT 
AGCAGTCTGC TGGCCAGCCA AGATGAGACA AAGTTGCCTA AAATAGACTT TTTTGACTAT 
TCTAAATTGA CCCCTCTTGA CCAGCGCTGC TTCATCCAAG CTGCTGACCT CCTCATGGCC 
GACTTCAAAG TGCTCAGTAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTGTCTGAT GCCATTAAAA AATGGCAGGA GCTGTCACCA 
GAAACCAGTG GAAAAAGGAA GAAGAGAAAA CAAATGAACC AGTATTCTTA CATTGATTTC 
AAGTTTGAAC AAGGTGACAT AAAAATAGAA AAGAGGATGT TCTTTCTTGA AAATAAGCGA 
CGACATTGTA GGTCCTATGA CCGACGTGCT CTCCTTCCAG CTGTGCAACA AGAGCAGGAG 
TTCTATGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTACAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGTCGCTG CTGCTATGGG 
GAATTTCCAT TCGAGGAGCT GACGCAGTGC GCAGATGCTC ACTTGTTCTG CAAAGAGTGT 
CTCATCAGAT ATGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTTGGAGCT CAGCTGCATG 
GAAGGCAGCT GCACGTGTTC GTTCCCAACC AGTGAGCTGG AGAAGGTGCT CCCCCAGACC 
ATCCTGTATA AGTACTATGA GCGAAAAGCC GAGGAGGAGG TTGCGGCAGC CTACGCCGAC 
GAGCTTGTCA GGTGCCCGTC CTGTAGCTTT CCGGCTCTGT TGGACAGTGA TGTGAAGAGG 
TTCAGCTGTC CTAATCCTCA CTGCCGAAAG GAAACCTGTA GGAAGTGTCA GGGACTCTGG 
AAAGAACATA ATGGCCTCAC CTGTGAAGAG CTGGCTGAAA AAGACGACAT CAAGTACCGT 
ACCTCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGAA AATGCCACAA GTGTGGGACT 
GGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCTTGCC GCTGTGGTGC CCAGATGTGC 
TACCTCTGTC GAGTTTCTAT TAATGGATAT GACCATTTCT GCCAACATCC CCGCTCACCA 
GGAGCCCCTT GCCAGGAGTG TTCAAGATGC TCTCTCTGGA CCGATCCCAC TGAAGATGAT 
GAGAAGCTTA TTGAGGAAAT CCAGAAGGAG GCTGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT TGGACCCCCG CTGGAGAAGC CTGTGGAGAA GGTGCAGAGG 
GTGGAGGCCC TCCCGAGGCC CGTTCCGCAG AACCTGCCAC AGCCACAGAT GCCACCCTAT 
GCCTTCGCGC ACCCACCCTT CCCCCTGCCT CCCGTGCGGC CTGTGTTCAA CAACTTCCCA 
CTCAACATGG GGCCTATCCC AGCCCCGTAC GTGCCCCCTC TGCCCAACGT GCGGGTCAAC 
TATGACTTCG GTCCCATCCA CATGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCGCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( NM_207111.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_996994.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22477
Clone ID Related Accession (Same CDS sequence)	NM_207111.3 , XM_005249785.2 , NM_207111.4 , XM_024446805.1
Accession Version	NM_207111.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008167.5 and AC006483.3. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_207111.3. Transcript Variant: This variant (1, also known as TRIAD3B) represents the longer transcript and it encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.64605.1, SRR1803612.39934.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AACAAATAAA CCTCAGAGAT CACGACCCAA TCTCATCAAA 
CCAGCTGCCC AGTGGCAAGA TCTGAAAAGG TTGGGAGAAG AAAGGCCTAA AAAGTCTAGA 
GCAGCATTTG AATCAGATAA GAGCAGCTAT TTTTCAGTGT GTAACAACCC ATTGTTTGAT 
TCTGGGGCAC AGGATGATTC TGAGGATGAC TACGGTGAAT TTCTGGATCT TGGGCCTCCT 
GGAATCTCTG AATTCACTAA GCCAAGTGGC CAAACAGAAA GAGAACCCAA GCCTGGACCG 
AGTCATAACC AAGCAGCAAA TGACATTGTC AACCCCAGAT CAGAGCAGAA AGTCATCATC 
TTGGAAGAAG GTAGCCTTCT TTACACAGAA AGCGATCCTT TGGAAACTCA GAACCAGTCA 
TCCGAAGACT CAGAGACAGA GCTGTTATCA AATCTAGGAG AGTCAGCTGC TCTAGCAGAT 
GATCAGGCCA TCGAAGAAGA CTGCTGGTTA GATCATCCTT ACTTCCAGTC TCTGAACCAA 
CAGCCCCGTG AAATAACAAA CCAGGTCGTT CCTCAGGAAC GGCAGCCTGA AGCAGAACTG 
GGCCGCTTGT TGTTTCAGCA TGAATTCCCA GGGCCCGCTT TTCCAAGGCC GGAACCCCAG 
CAAGGTGGGA TTTCAGGCCC CTCTTCTCCT CAGCCTGCCC ATCCTCTAGG AGAGTTTGAA 
GACCAGCAGT TAGCAAGTGA TGATGAAGAG CCAGGTCCAG CCTTTCCAAT GCAAGAATCT 
CAAGAGCCCA ATTTGGAAAA CATTTGGGGG CAAGAAGCTG CAGAGGTAGA TCAAGAGCTC 
GTTGAACTAC TAGTGAAAGA AACGGAAGCA AGATTTCCAG ATGTAGCAAA TGGGTTTATT 
GAGGAAATAA TTCATTTTAA GAATTATTAT GATCTGAATG TACTTTGTAA TTTTCTTCTG 
GAAAACCCAG ATTATCCAAA GAGAGAAGAC AGAATCATTA TAAATCCCAG TAGCAGTCTG 
CTGGCCAGCC AAGATGAGAC AAAGTTGCCT AAAATAGACT TTTTTGACTA TTCTAAATTG 
ACCCCTCTTG ACCAGCGCTG CTTCATCCAA GCTGCTGACC TCCTCATGGC CGACTTCAAA 
GTGCTCAGTA GTCAGGACAT CAAGTGGGCC CTGCACGAGC TCAAAGGACA CTATGCAATC 
ACCCGAAAGG CCTTGTCTGA TGCCATTAAA AAATGGCAGG AGCTGTCACC AGAAACCAGT 
GGAAAAAGGA AGAAGAGAAA ACAAATGAAC CAGTATTCTT ACATTGATTT CAAGTTTGAA 
CAAGGTGACA TAAAAATAGA AAAGAGGATG TTCTTTCTTG AAAATAAGCG ACGACATTGT 
AGGTCCTATG ACCGACGTGC TCTCCTTCCA GCTGTGCAAC AAGAGCAGGA GTTCTATGAG 
CAGAAAATCA AAGAGATGGC AGAGCATGAA GACTTTTTGC TTGCCCTACA GATGAATGAA 
GAACAGTATC AAAAGGATGG CCAGCTGATT GAGTGTCGCT GCTGCTATGG GGAATTTCCA 
TTCGAGGAGC TGACGCAGTG CGCAGATGCT CACTTGTTCT GCAAAGAGTG TCTCATCAGA 
TATGCCCAAG AGGCAGTCTT TGGATCTGGA AAGTTGGAGC TCAGCTGCAT GGAAGGCAGC 
TGCACGTGTT CGTTCCCAAC CAGTGAGCTG GAGAAGGTGC TCCCCCAGAC CATCCTGTAT 
AAGTACTATG AGCGAAAAGC CGAGGAGGAG GTTGCGGCAG CCTACGCCGA CGAGCTTGTC 
AGGTGCCCGT CCTGTAGCTT TCCGGCTCTG TTGGACAGTG ATGTGAAGAG GTTCAGCTGT 
CCTAATCCTC ACTGCCGAAA GGAAACCTGT AGGAAGTGTC AGGGACTCTG GAAAGAACAT 
AATGGCCTCA CCTGTGAAGA GCTGGCTGAA AAAGACGACA TCAAGTACCG TACCTCTATT 
GAAGAAAAAA TGACTGCTGC CCGCATTAGA AAATGCCACA AGTGTGGGAC TGGCCTCATC 
AAATCTGAAG GCTGCAACCG CATGTCTTGC CGCTGTGGTG CCCAGATGTG CTACCTCTGT 
CGAGTTTCTA TTAATGGATA TGACCATTTC TGCCAACATC CCCGCTCACC AGGAGCCCCT 
TGCCAGGAGT GTTCAAGATG CTCTCTCTGG ACCGATCCCA CTGAAGATGA TGAGAAGCTT 
ATTGAGGAAA TCCAGAAGGA GGCTGAAGAG GAACAGAAAA GAAAGAATGG AGAGAACACC 
TTCAAACGCA TTGGACCCCC GCTGGAGAAG CCTGTGGAGA AGGTGCAGAG GGTGGAGGCC 
CTCCCGAGGC CCGTTCCGCA GAACCTGCCA CAGCCACAGA TGCCACCCTA TGCCTTCGCG 
CACCCACCCT TCCCCCTGCC TCCCGTGCGG CCTGTGTTCA ACAACTTCCC ACTCAACATG 
GGGCCTATCC CAGCCCCGTA CGTGCCCCCT CTGCCCAACG TGCGGGTCAA CTATGACTTC 
GGTCCCATCC ACATGCCCCT GGAGCACAAC CTGCCCATGC ACTTTGGCCC CCAGCCGCGG 
CATCGCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996994.1
CDS	193..2964
Translation	MEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIPMLVTPAPQQHEEEDLDDDVILTETNKPQRSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDKSSYFSVCNNPLFDSGAQDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGPSHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAALADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPRPEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAAEVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRIIINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	NM_207111.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207111.4

ORF Insert Sequence:

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AACAAATAAA CCTCAGAGAT CACGACCCAA TCTCATCAAA 
CCAGCTGCCC AGTGGCAAGA TCTGAAAAGG TTGGGAGAAG AAAGGCCTAA AAAGTCTAGA 
GCAGCATTTG AATCAGATAA GAGCAGCTAT TTTTCAGTGT GTAACAACCC ATTGTTTGAT 
TCTGGGGCAC AGGATGATTC TGAGGATGAC TACGGTGAAT TTCTGGATCT TGGGCCTCCT 
GGAATCTCTG AATTCACTAA GCCAAGTGGC CAAACAGAAA GAGAACCCAA GCCTGGACCG 
AGTCATAACC AAGCAGCAAA TGACATTGTC AACCCCAGAT CAGAGCAGAA AGTCATCATC 
TTGGAAGAAG GTAGCCTTCT TTACACAGAA AGCGATCCTT TGGAAACTCA GAACCAGTCA 
TCCGAAGACT CAGAGACAGA GCTGTTATCA AATCTAGGAG AGTCAGCTGC TCTAGCAGAT 
GATCAGGCCA TCGAAGAAGA CTGCTGGTTA GATCATCCTT ACTTCCAGTC TCTGAACCAA 
CAGCCCCGTG AAATAACAAA CCAGGTCGTT CCTCAGGAAC GGCAGCCTGA AGCAGAACTG 
GGCCGCTTGT TGTTTCAGCA TGAATTCCCA GGGCCCGCTT TTCCAAGGCC GGAACCCCAG 
CAAGGTGGGA TTTCAGGCCC CTCTTCTCCT CAGCCTGCCC ATCCTCTAGG AGAGTTTGAA 
GACCAGCAGT TAGCAAGTGA TGATGAAGAG CCAGGTCCAG CCTTTCCAAT GCAAGAATCT 
CAAGAGCCCA ATTTGGAAAA CATTTGGGGG CAAGAAGCTG CAGAGGTAGA TCAAGAGCTC 
GTTGAACTAC TAGTGAAAGA AACGGAAGCA AGATTTCCAG ATGTAGCAAA TGGGTTTATT 
GAGGAAATAA TTCATTTTAA GAATTATTAT GATCTGAATG TACTTTGTAA TTTTCTTCTG 
GAAAACCCAG ATTATCCAAA GAGAGAAGAC AGAATCATTA TAAATCCCAG TAGCAGTCTG 
CTGGCCAGCC AAGATGAGAC AAAGTTGCCT AAAATAGACT TTTTTGACTA TTCTAAATTG 
ACCCCTCTTG ACCAGCGCTG CTTCATCCAA GCTGCTGACC TCCTCATGGC CGACTTCAAA 
GTGCTCAGTA GTCAGGACAT CAAGTGGGCC CTGCACGAGC TCAAAGGACA CTATGCAATC 
ACCCGAAAGG CCTTGTCTGA TGCCATTAAA AAATGGCAGG AGCTGTCACC AGAAACCAGT 
GGAAAAAGGA AGAAGAGAAA ACAAATGAAC CAGTATTCTT ACATTGATTT CAAGTTTGAA 
CAAGGTGACA TAAAAATAGA AAAGAGGATG TTCTTTCTTG AAAATAAGCG ACGACATTGT 
AGGTCCTATG ACCGACGTGC TCTCCTTCCA GCTGTGCAAC AAGAGCAGGA GTTCTATGAG 
CAGAAAATCA AAGAGATGGC AGAGCATGAA GACTTTTTGC TTGCCCTACA GATGAATGAA 
GAACAGTATC AAAAGGATGG CCAGCTGATT GAGTGTCGCT GCTGCTATGG GGAATTTCCA 
TTCGAGGAGC TGACGCAGTG CGCAGATGCT CACTTGTTCT GCAAAGAGTG TCTCATCAGA 
TATGCCCAAG AGGCAGTCTT TGGATCTGGA AAGTTGGAGC TCAGCTGCAT GGAAGGCAGC 
TGCACGTGTT CGTTCCCAAC CAGTGAGCTG GAGAAGGTGC TCCCCCAGAC CATCCTGTAT 
AAGTACTATG AGCGAAAAGC CGAGGAGGAG GTTGCGGCAG CCTACGCCGA CGAGCTTGTC 
AGGTGCCCGT CCTGTAGCTT TCCGGCTCTG TTGGACAGTG ATGTGAAGAG GTTCAGCTGT 
CCTAATCCTC ACTGCCGAAA GGAAACCTGT AGGAAGTGTC AGGGACTCTG GAAAGAACAT 
AATGGCCTCA CCTGTGAAGA GCTGGCTGAA AAAGACGACA TCAAGTACCG TACCTCTATT 
GAAGAAAAAA TGACTGCTGC CCGCATTAGA AAATGCCACA AGTGTGGGAC TGGCCTCATC 
AAATCTGAAG GCTGCAACCG CATGTCTTGC CGCTGTGGTG CCCAGATGTG CTACCTCTGT 
CGAGTTTCTA TTAATGGATA TGACCATTTC TGCCAACATC CCCGCTCACC AGGAGCCCCT 
TGCCAGGAGT GTTCAAGATG CTCTCTCTGG ACCGATCCCA CTGAAGATGA TGAGAAGCTT 
ATTGAGGAAA TCCAGAAGGA GGCTGAAGAG GAACAGAAAA GAAAGAATGG AGAGAACACC 
TTCAAACGCA TTGGACCCCC GCTGGAGAAG CCTGTGGAGA AGGTGCAGAG GGTGGAGGCC 
CTCCCGAGGC CCGTTCCGCA GAACCTGCCA CAGCCACAGA TGCCACCCTA TGCCTTCGCG 
CACCCACCCT TCCCCCTGCC TCCCGTGCGG CCTGTGTTCA ACAACTTCCC ACTCAACATG 
GGGCCTATCC CAGCCCCGTA CGTGCCCCCT CTGCCCAACG TGCGGGTCAA CTATGACTTC 
GGTCCCATCC ACATGCCCCT GGAGCACAAC CTGCCCATGC ACTTTGGCCC CCAGCCGCGG 
CATCGCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_017012363.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867852.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu08423
Clone ID Related Accession (Same CDS sequence)	XM_017012363.1 , XM_017012363.2 , NM_207116.3 , NM_207116.2 , NM_001377156.1
Accession Version	XM_017012363.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AGATGATTCT GAGGATGACT ACGGTGAATT TCTGGATCTT 
GGGCCTCCTG GAATCTCTGA ATTCACTAAG CCAAGTGGCC AAACAGAAAG AGAACCCAAG 
CCTGGACCGA GTCATAACCA AGCAGCAAAT GACATTGTCA ACCCCAGATC AGAGCAGAAA 
GTCATCATCT TGGAAGAAGG TAGCCTTCTT TACACAGAAA GCGATCCTTT GGAAACTCAG 
AACCAGTCAT CCGAAGACTC AGAGACAGAG CTGTTATCAA ATCTAGGAGA GTCAGCTGCT 
CTAGCAGATG ATCAGGCCAT CGAAGAAGAC TGCTGGTTAG ATCATCCTTA CTTCCAGTCT 
CTGAACCAAC AGCCCCGTGA AATAACAAAC CAGGTCGTTC CTCAGGAACG GCAGCCTGAA 
GCAGAACTGG GCCGCTTGTT GTTTCAGCAT GAATTCCCAG GGCCCGCTTT TCCAAGGCCG 
GAACCCCAGC AAGGTGGGAT TTCAGGCCCC TCTTCTCCTC AGCCTGCCCA TCCTCTAGGA 
GAGTTTGAAG ACCAGCAGTT AGCAAGTGAT GATGAAGAGC CAGGTCCAGC CTTTCCAATG 
CAAGAATCTC AAGAGCCCAA TTTGGAAAAC ATTTGGGGGC AAGAAGCTGC AGAGGTAGAT 
CAAGAGCTCG TTGAACTACT AGTGAAAGAA ACGGAAGCAA GATTTCCAGA TGTAGCAAAT 
GGGTTTATTG AGGAAATAAT TCATTTTAAG AATTATTATG ATCTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAACCCAGA TTATCCAAAG AGAGAAGACA GAATCATTAT AAATCCCAGT 
AGCAGTCTGC TGGCCAGCCA AGATGAGACA AAGTTGCCTA AAATAGACTT TTTTGACTAT 
TCTAAATTGA CCCCTCTTGA CCAGCGCTGC TTCATCCAAG CTGCTGACCT CCTCATGGCC 
GACTTCAAAG TGCTCAGTAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTGTCTGAT GCCATTAAAA AATGGCAGGA GCTGTCACCA 
GAAACCAGTG GAAAAAGGAA GAAGAGAAAA CAAATGAACC AGTATTCTTA CATTGATTTC 
AAGTTTGAAC AAGGTGACAT AAAAATAGAA AAGAGGATGT TCTTTCTTGA AAATAAGCGA 
CGACATTGTA GGTCCTATGA CCGACGTGCT CTCCTTCCAG CTGTGCAACA AGAGCAGGAG 
TTCTATGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTACAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGTCGCTG CTGCTATGGG 
GAATTTCCAT TCGAGGAGCT GACGCAGTGC GCAGATGCTC ACTTGTTCTG CAAAGAGTGT 
CTCATCAGAT ATGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTTGGAGCT CAGCTGCATG 
GAAGGCAGCT GCACGTGTTC GTTCCCAACC AGTGAGCTGG AGAAGGTGCT CCCCCAGACC 
ATCCTGTATA AGTACTATGA GCGAAAAGCC GAGGAGGAGG TTGCGGCAGC CTACGCCGAC 
GAGCTTGTCA GGTGCCCGTC CTGTAGCTTT CCGGCTCTGT TGGACAGTGA TGTGAAGAGG 
TTCAGCTGTC CTAATCCTCA CTGCCGAAAG GAAACCTGTA GGAAGTGTCA GGGACTCTGG 
AAAGAACATA ATGGCCTCAC CTGTGAAGAG CTGGCTGAAA AAGACGACAT CAAGTACCGT 
ACCTCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGAA AATGCCACAA GTGTGGGACT 
GGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCTTGCC GCTGTGGTGC CCAGATGTGC 
TACCTCTGTC GAGTTTCTAT TAATGGATAT GACCATTTCT GCCAACATCC CCGCTCACCA 
GGAGCCCCTT GCCAGGAGTG TTCAAGATGC TCTCTCTGGA CCGATCCCAC TGAAGATGAT 
GAGAAGCTTA TTGAGGAAAT CCAGAAGGAG GCTGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT TGGACCCCCG CTGGAGAAGC CTGTGGAGAA GGTGCAGAGG 
GTGGAGGCCC TCCCGAGGCC CGTTCCGCAG AACCTGCCAC AGCCACAGAT GCCACCCTAT 
GCCTTCGCGC ACCCACCCTT CCCCCTGCCT CCCGTGCGGC CTGTGTTCAA CAACTTCCCA 
CTCAACATGG GGCCTATCCC AGCCCCGTAC GTGCCCCCTC TGCCCAACGT GCGGGTCAAC 
TATGACTTCG GTCCCATCCA CATGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCGCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867852.1
CDS	115..2715
Translation	MEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIPMLVTPAPQQHEEEDLDDDVILTEDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGPSHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAALADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPRPEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAAEVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRIIINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_017012363.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012363.1

ORF Insert Sequence:

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AGATGATTCT GAGGATGACT ACGGTGAATT TCTGGATCTT 
GGGCCTCCTG GAATCTCTGA ATTCACTAAG CCAAGTGGCC AAACAGAAAG AGAACCCAAG 
CCTGGACCGA GTCATAACCA AGCAGCAAAT GACATTGTCA ACCCCAGATC AGAGCAGAAA 
GTCATCATCT TGGAAGAAGG TAGCCTTCTT TACACAGAAA GCGATCCTTT GGAAACTCAG 
AACCAGTCAT CCGAAGACTC AGAGACAGAG CTGTTATCAA ATCTAGGAGA GTCAGCTGCT 
CTAGCAGATG ATCAGGCCAT CGAAGAAGAC TGCTGGTTAG ATCATCCTTA CTTCCAGTCT 
CTGAACCAAC AGCCCCGTGA AATAACAAAC CAGGTCGTTC CTCAGGAACG GCAGCCTGAA 
GCAGAACTGG GCCGCTTGTT GTTTCAGCAT GAATTCCCAG GGCCCGCTTT TCCAAGGCCG 
GAACCCCAGC AAGGTGGGAT TTCAGGCCCC TCTTCTCCTC AGCCTGCCCA TCCTCTAGGA 
GAGTTTGAAG ACCAGCAGTT AGCAAGTGAT GATGAAGAGC CAGGTCCAGC CTTTCCAATG 
CAAGAATCTC AAGAGCCCAA TTTGGAAAAC ATTTGGGGGC AAGAAGCTGC AGAGGTAGAT 
CAAGAGCTCG TTGAACTACT AGTGAAAGAA ACGGAAGCAA GATTTCCAGA TGTAGCAAAT 
GGGTTTATTG AGGAAATAAT TCATTTTAAG AATTATTATG ATCTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAACCCAGA TTATCCAAAG AGAGAAGACA GAATCATTAT AAATCCCAGT 
AGCAGTCTGC TGGCCAGCCA AGATGAGACA AAGTTGCCTA AAATAGACTT TTTTGACTAT 
TCTAAATTGA CCCCTCTTGA CCAGCGCTGC TTCATCCAAG CTGCTGACCT CCTCATGGCC 
GACTTCAAAG TGCTCAGTAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTGTCTGAT GCCATTAAAA AATGGCAGGA GCTGTCACCA 
GAAACCAGTG GAAAAAGGAA GAAGAGAAAA CAAATGAACC AGTATTCTTA CATTGATTTC 
AAGTTTGAAC AAGGTGACAT AAAAATAGAA AAGAGGATGT TCTTTCTTGA AAATAAGCGA 
CGACATTGTA GGTCCTATGA CCGACGTGCT CTCCTTCCAG CTGTGCAACA AGAGCAGGAG 
TTCTATGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTACAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGTCGCTG CTGCTATGGG 
GAATTTCCAT TCGAGGAGCT GACGCAGTGC GCAGATGCTC ACTTGTTCTG CAAAGAGTGT 
CTCATCAGAT ATGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTTGGAGCT CAGCTGCATG 
GAAGGCAGCT GCACGTGTTC GTTCCCAACC AGTGAGCTGG AGAAGGTGCT CCCCCAGACC 
ATCCTGTATA AGTACTATGA GCGAAAAGCC GAGGAGGAGG TTGCGGCAGC CTACGCCGAC 
GAGCTTGTCA GGTGCCCGTC CTGTAGCTTT CCGGCTCTGT TGGACAGTGA TGTGAAGAGG 
TTCAGCTGTC CTAATCCTCA CTGCCGAAAG GAAACCTGTA GGAAGTGTCA GGGACTCTGG 
AAAGAACATA ATGGCCTCAC CTGTGAAGAG CTGGCTGAAA AAGACGACAT CAAGTACCGT 
ACCTCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGAA AATGCCACAA GTGTGGGACT 
GGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCTTGCC GCTGTGGTGC CCAGATGTGC 
TACCTCTGTC GAGTTTCTAT TAATGGATAT GACCATTTCT GCCAACATCC CCGCTCACCA 
GGAGCCCCTT GCCAGGAGTG TTCAAGATGC TCTCTCTGGA CCGATCCCAC TGAAGATGAT 
GAGAAGCTTA TTGAGGAAAT CCAGAAGGAG GCTGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT TGGACCCCCG CTGGAGAAGC CTGTGGAGAA GGTGCAGAGG 
GTGGAGGCCC TCCCGAGGCC CGTTCCGCAG AACCTGCCAC AGCCACAGAT GCCACCCTAT 
GCCTTCGCGC ACCCACCCTT CCCCCTGCCT CCCGTGCGGC CTGTGTTCAA CAACTTCCCA 
CTCAACATGG GGCCTATCCC AGCCCCGTAC GTGCCCCCTC TGCCCAACGT GCGGGTCAAC 
TATGACTTCG GTCCCATCCA CATGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCGCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_017012363.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867852.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu08423
Clone ID Related Accession (Same CDS sequence)	XM_017012363.1 , XM_017012363.2 , NM_207116.3 , NM_207116.2 , NM_001377156.1
Accession Version	XM_017012363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017012363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AGATGATTCT GAGGATGACT ACGGTGAATT TCTGGATCTT 
GGGCCTCCTG GAATCTCTGA ATTCACTAAG CCAAGTGGCC AAACAGAAAG AGAACCCAAG 
CCTGGACCGA GTCATAACCA AGCAGCAAAT GACATTGTCA ACCCCAGATC AGAGCAGAAA 
GTCATCATCT TGGAAGAAGG TAGCCTTCTT TACACAGAAA GCGATCCTTT GGAAACTCAG 
AACCAGTCAT CCGAAGACTC AGAGACAGAG CTGTTATCAA ATCTAGGAGA GTCAGCTGCT 
CTAGCAGATG ATCAGGCCAT CGAAGAAGAC TGCTGGTTAG ATCATCCTTA CTTCCAGTCT 
CTGAACCAAC AGCCCCGTGA AATAACAAAC CAGGTCGTTC CTCAGGAACG GCAGCCTGAA 
GCAGAACTGG GCCGCTTGTT GTTTCAGCAT GAATTCCCAG GGCCCGCTTT TCCAAGGCCG 
GAACCCCAGC AAGGTGGGAT TTCAGGCCCC TCTTCTCCTC AGCCTGCCCA TCCTCTAGGA 
GAGTTTGAAG ACCAGCAGTT AGCAAGTGAT GATGAAGAGC CAGGTCCAGC CTTTCCAATG 
CAAGAATCTC AAGAGCCCAA TTTGGAAAAC ATTTGGGGGC AAGAAGCTGC AGAGGTAGAT 
CAAGAGCTCG TTGAACTACT AGTGAAAGAA ACGGAAGCAA GATTTCCAGA TGTAGCAAAT 
GGGTTTATTG AGGAAATAAT TCATTTTAAG AATTATTATG ATCTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAACCCAGA TTATCCAAAG AGAGAAGACA GAATCATTAT AAATCCCAGT 
AGCAGTCTGC TGGCCAGCCA AGATGAGACA AAGTTGCCTA AAATAGACTT TTTTGACTAT 
TCTAAATTGA CCCCTCTTGA CCAGCGCTGC TTCATCCAAG CTGCTGACCT CCTCATGGCC 
GACTTCAAAG TGCTCAGTAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTGTCTGAT GCCATTAAAA AATGGCAGGA GCTGTCACCA 
GAAACCAGTG GAAAAAGGAA GAAGAGAAAA CAAATGAACC AGTATTCTTA CATTGATTTC 
AAGTTTGAAC AAGGTGACAT AAAAATAGAA AAGAGGATGT TCTTTCTTGA AAATAAGCGA 
CGACATTGTA GGTCCTATGA CCGACGTGCT CTCCTTCCAG CTGTGCAACA AGAGCAGGAG 
TTCTATGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTACAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGTCGCTG CTGCTATGGG 
GAATTTCCAT TCGAGGAGCT GACGCAGTGC GCAGATGCTC ACTTGTTCTG CAAAGAGTGT 
CTCATCAGAT ATGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTTGGAGCT CAGCTGCATG 
GAAGGCAGCT GCACGTGTTC GTTCCCAACC AGTGAGCTGG AGAAGGTGCT CCCCCAGACC 
ATCCTGTATA AGTACTATGA GCGAAAAGCC GAGGAGGAGG TTGCGGCAGC CTACGCCGAC 
GAGCTTGTCA GGTGCCCGTC CTGTAGCTTT CCGGCTCTGT TGGACAGTGA TGTGAAGAGG 
TTCAGCTGTC CTAATCCTCA CTGCCGAAAG GAAACCTGTA GGAAGTGTCA GGGACTCTGG 
AAAGAACATA ATGGCCTCAC CTGTGAAGAG CTGGCTGAAA AAGACGACAT CAAGTACCGT 
ACCTCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGAA AATGCCACAA GTGTGGGACT 
GGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCTTGCC GCTGTGGTGC CCAGATGTGC 
TACCTCTGTC GAGTTTCTAT TAATGGATAT GACCATTTCT GCCAACATCC CCGCTCACCA 
GGAGCCCCTT GCCAGGAGTG TTCAAGATGC TCTCTCTGGA CCGATCCCAC TGAAGATGAT 
GAGAAGCTTA TTGAGGAAAT CCAGAAGGAG GCTGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT TGGACCCCCG CTGGAGAAGC CTGTGGAGAA GGTGCAGAGG 
GTGGAGGCCC TCCCGAGGCC CGTTCCGCAG AACCTGCCAC AGCCACAGAT GCCACCCTAT 
GCCTTCGCGC ACCCACCCTT CCCCCTGCCT CCCGTGCGGC CTGTGTTCAA CAACTTCCCA 
CTCAACATGG GGCCTATCCC AGCCCCGTAC GTGCCCCCTC TGCCCAACGT GCGGGTCAAC 
TATGACTTCG GTCCCATCCA CATGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCGCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867852.1
CDS	255..2855
Translation	MEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIPMLVTPAPQQHEEEDLDDDVILTEDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGPSHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAALADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPRPEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAAEVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRIIINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_017012363.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012363.2

ORF Insert Sequence:

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AGATGATTCT GAGGATGACT ACGGTGAATT TCTGGATCTT 
GGGCCTCCTG GAATCTCTGA ATTCACTAAG CCAAGTGGCC AAACAGAAAG AGAACCCAAG 
CCTGGACCGA GTCATAACCA AGCAGCAAAT GACATTGTCA ACCCCAGATC AGAGCAGAAA 
GTCATCATCT TGGAAGAAGG TAGCCTTCTT TACACAGAAA GCGATCCTTT GGAAACTCAG 
AACCAGTCAT CCGAAGACTC AGAGACAGAG CTGTTATCAA ATCTAGGAGA GTCAGCTGCT 
CTAGCAGATG ATCAGGCCAT CGAAGAAGAC TGCTGGTTAG ATCATCCTTA CTTCCAGTCT 
CTGAACCAAC AGCCCCGTGA AATAACAAAC CAGGTCGTTC CTCAGGAACG GCAGCCTGAA 
GCAGAACTGG GCCGCTTGTT GTTTCAGCAT GAATTCCCAG GGCCCGCTTT TCCAAGGCCG 
GAACCCCAGC AAGGTGGGAT TTCAGGCCCC TCTTCTCCTC AGCCTGCCCA TCCTCTAGGA 
GAGTTTGAAG ACCAGCAGTT AGCAAGTGAT GATGAAGAGC CAGGTCCAGC CTTTCCAATG 
CAAGAATCTC AAGAGCCCAA TTTGGAAAAC ATTTGGGGGC AAGAAGCTGC AGAGGTAGAT 
CAAGAGCTCG TTGAACTACT AGTGAAAGAA ACGGAAGCAA GATTTCCAGA TGTAGCAAAT 
GGGTTTATTG AGGAAATAAT TCATTTTAAG AATTATTATG ATCTGAATGT ACTTTGTAAT 
TTTCTTCTGG AAAACCCAGA TTATCCAAAG AGAGAAGACA GAATCATTAT AAATCCCAGT 
AGCAGTCTGC TGGCCAGCCA AGATGAGACA AAGTTGCCTA AAATAGACTT TTTTGACTAT 
TCTAAATTGA CCCCTCTTGA CCAGCGCTGC TTCATCCAAG CTGCTGACCT CCTCATGGCC 
GACTTCAAAG TGCTCAGTAG TCAGGACATC AAGTGGGCCC TGCACGAGCT CAAAGGACAC 
TATGCAATCA CCCGAAAGGC CTTGTCTGAT GCCATTAAAA AATGGCAGGA GCTGTCACCA 
GAAACCAGTG GAAAAAGGAA GAAGAGAAAA CAAATGAACC AGTATTCTTA CATTGATTTC 
AAGTTTGAAC AAGGTGACAT AAAAATAGAA AAGAGGATGT TCTTTCTTGA AAATAAGCGA 
CGACATTGTA GGTCCTATGA CCGACGTGCT CTCCTTCCAG CTGTGCAACA AGAGCAGGAG 
TTCTATGAGC AGAAAATCAA AGAGATGGCA GAGCATGAAG ACTTTTTGCT TGCCCTACAG 
ATGAATGAAG AACAGTATCA AAAGGATGGC CAGCTGATTG AGTGTCGCTG CTGCTATGGG 
GAATTTCCAT TCGAGGAGCT GACGCAGTGC GCAGATGCTC ACTTGTTCTG CAAAGAGTGT 
CTCATCAGAT ATGCCCAAGA GGCAGTCTTT GGATCTGGAA AGTTGGAGCT CAGCTGCATG 
GAAGGCAGCT GCACGTGTTC GTTCCCAACC AGTGAGCTGG AGAAGGTGCT CCCCCAGACC 
ATCCTGTATA AGTACTATGA GCGAAAAGCC GAGGAGGAGG TTGCGGCAGC CTACGCCGAC 
GAGCTTGTCA GGTGCCCGTC CTGTAGCTTT CCGGCTCTGT TGGACAGTGA TGTGAAGAGG 
TTCAGCTGTC CTAATCCTCA CTGCCGAAAG GAAACCTGTA GGAAGTGTCA GGGACTCTGG 
AAAGAACATA ATGGCCTCAC CTGTGAAGAG CTGGCTGAAA AAGACGACAT CAAGTACCGT 
ACCTCTATTG AAGAAAAAAT GACTGCTGCC CGCATTAGAA AATGCCACAA GTGTGGGACT 
GGCCTCATCA AATCTGAAGG CTGCAACCGC ATGTCTTGCC GCTGTGGTGC CCAGATGTGC 
TACCTCTGTC GAGTTTCTAT TAATGGATAT GACCATTTCT GCCAACATCC CCGCTCACCA 
GGAGCCCCTT GCCAGGAGTG TTCAAGATGC TCTCTCTGGA CCGATCCCAC TGAAGATGAT 
GAGAAGCTTA TTGAGGAAAT CCAGAAGGAG GCTGAAGAGG AACAGAAAAG AAAGAATGGA 
GAGAACACCT TCAAACGCAT TGGACCCCCG CTGGAGAAGC CTGTGGAGAA GGTGCAGAGG 
GTGGAGGCCC TCCCGAGGCC CGTTCCGCAG AACCTGCCAC AGCCACAGAT GCCACCCTAT 
GCCTTCGCGC ACCCACCCTT CCCCCTGCCT CCCGTGCGGC CTGTGTTCAA CAACTTCCCA 
CTCAACATGG GGCCTATCCC AGCCCCGTAC GTGCCCCCTC TGCCCAACGT GCGGGTCAAC 
TATGACTTCG GTCCCATCCA CATGCCCCTG GAGCACAACC TGCCCATGCA CTTTGGCCCC 
CAGCCGCGGC ATCGCTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_017012365.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867854.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu41206
Clone ID Related Accession (Same CDS sequence)	XM_017012365.1 , XM_011515436.1 , XM_024446806.1 , XM_011515436.2 , XM_024446807.1
Accession Version	XM_017012365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGACT TCAAAGTGCT CAGTAGTCAG GACATCAAGT GGGCCCTGCA CGAGCTCAAA 
GGACACTATG CAATCACCCG AAAGGCCTTG TCTGATGCCA TTAAAAAATG GCAGGAGCTG 
TCACCAGAAA CCAGTGGAAA AAGGAAGAAG AGAAAACAAA TGAACCAGTA TTCTTACATT 
GATTTCAAGT TTGAACAAGG TGACATAAAA ATAGAAAAGA GGATGTTCTT TCTTGAAAAT 
AAGCGACGAC ATTGTAGGTC CTATGACCGA CGTGCTCTCC TTCCAGCTGT GCAACAAGAG 
CAGGAGTTCT ATGAGCAGAA AATCAAAGAG ATGGCAGAGC ATGAAGACTT TTTGCTTGCC 
CTACAGATGA ATGAAGAACA GTATCAAAAG GATGGCCAGC TGATTGAGTG TCGCTGCTGC 
TATGGGGAAT TTCCATTCGA GGAGCTGACG CAGTGCGCAG ATGCTCACTT GTTCTGCAAA 
GAGTGTCTCA TCAGATATGC CCAAGAGGCA GTCTTTGGAT CTGGAAAGTT GGAGCTCAGC 
TGCATGGAAG GCAGCTGCAC GTGTTCGTTC CCAACCAGTG AGCTGGAGAA GGTGCTCCCC 
CAGACCATCC TGTATAAGTA CTATGAGCGA AAAGCCGAGG AGGAGGTTGC GGCAGCCTAC 
GCCGACGAGC TTGTCAGGTG CCCGTCCTGT AGCTTTCCGG CTCTGTTGGA CAGTGATGTG 
AAGAGGTTCA GCTGTCCTAA TCCTCACTGC CGAAAGGAAA CCTGTAGGAA GTGTCAGGGA 
CTCTGGAAAG AACATAATGG CCTCACCTGT GAAGAGCTGG CTGAAAAAGA CGACATCAAG 
TACCGTACCT CTATTGAAGA AAAAATGACT GCTGCCCGCA TTAGAAAATG CCACAAGTGT 
GGGACTGGCC TCATCAAATC TGAAGGCTGC AACCGCATGT CTTGCCGCTG TGGTGCCCAG 
ATGTGCTACC TCTGTCGAGT TTCTATTAAT GGATATGACC ATTTCTGCCA ACATCCCCGC 
TCACCAGGAG CCCCTTGCCA GGAGTGTTCA AGATGCTCTC TCTGGACCGA TCCCACTGAA 
GATGATGAGA AGCTTATTGA GGAAATCCAG AAGGAGGCTG AAGAGGAACA GAAAAGAAAG 
AATGGAGAGA ACACCTTCAA ACGCATTGGA CCCCCGCTGG AGAAGCCTGT GGAGAAGGTG 
CAGAGGGTGG AGGCCCTCCC GAGGCCCGTT CCGCAGAACC TGCCACAGCC ACAGATGCCA 
CCCTATGCCT TCGCGCACCC ACCCTTCCCC CTGCCTCCCG TGCGGCCTGT GTTCAACAAC 
TTCCCACTCA ACATGGGGCC TATCCCAGCC CCGTACGTGC CCCCTCTGCC CAACGTGCGG 
GTCAACTATG ACTTCGGTCC CATCCACATG CCCCTGGAGC ACAACCTGCC CATGCACTTT 
GGCCCCCAGC CGCGGCATCG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867854.1
CDS	1207..2673
Translation	MADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_017012365.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012365.1

ORF Insert Sequence:

ATGGCCGACT TCAAAGTGCT CAGTAGTCAG GACATCAAGT GGGCCCTGCA CGAGCTCAAA 
GGACACTATG CAATCACCCG AAAGGCCTTG TCTGATGCCA TTAAAAAATG GCAGGAGCTG 
TCACCAGAAA CCAGTGGAAA AAGGAAGAAG AGAAAACAAA TGAACCAGTA TTCTTACATT 
GATTTCAAGT TTGAACAAGG TGACATAAAA ATAGAAAAGA GGATGTTCTT TCTTGAAAAT 
AAGCGACGAC ATTGTAGGTC CTATGACCGA CGTGCTCTCC TTCCAGCTGT GCAACAAGAG 
CAGGAGTTCT ATGAGCAGAA AATCAAAGAG ATGGCAGAGC ATGAAGACTT TTTGCTTGCC 
CTACAGATGA ATGAAGAACA GTATCAAAAG GATGGCCAGC TGATTGAGTG TCGCTGCTGC 
TATGGGGAAT TTCCATTCGA GGAGCTGACG CAGTGCGCAG ATGCTCACTT GTTCTGCAAA 
GAGTGTCTCA TCAGATATGC CCAAGAGGCA GTCTTTGGAT CTGGAAAGTT GGAGCTCAGC 
TGCATGGAAG GCAGCTGCAC GTGTTCGTTC CCAACCAGTG AGCTGGAGAA GGTGCTCCCC 
CAGACCATCC TGTATAAGTA CTATGAGCGA AAAGCCGAGG AGGAGGTTGC GGCAGCCTAC 
GCCGACGAGC TTGTCAGGTG CCCGTCCTGT AGCTTTCCGG CTCTGTTGGA CAGTGATGTG 
AAGAGGTTCA GCTGTCCTAA TCCTCACTGC CGAAAGGAAA CCTGTAGGAA GTGTCAGGGA 
CTCTGGAAAG AACATAATGG CCTCACCTGT GAAGAGCTGG CTGAAAAAGA CGACATCAAG 
TACCGTACCT CTATTGAAGA AAAAATGACT GCTGCCCGCA TTAGAAAATG CCACAAGTGT 
GGGACTGGCC TCATCAAATC TGAAGGCTGC AACCGCATGT CTTGCCGCTG TGGTGCCCAG 
ATGTGCTACC TCTGTCGAGT TTCTATTAAT GGATATGACC ATTTCTGCCA ACATCCCCGC 
TCACCAGGAG CCCCTTGCCA GGAGTGTTCA AGATGCTCTC TCTGGACCGA TCCCACTGAA 
GATGATGAGA AGCTTATTGA GGAAATCCAG AAGGAGGCTG AAGAGGAACA GAAAAGAAAG 
AATGGAGAGA ACACCTTCAA ACGCATTGGA CCCCCGCTGG AGAAGCCTGT GGAGAAGGTG 
CAGAGGGTGG AGGCCCTCCC GAGGCCCGTT CCGCAGAACC TGCCACAGCC ACAGATGCCA 
CCCTATGCCT TCGCGCACCC ACCCTTCCCC CTGCCTCCCG TGCGGCCTGT GTTCAACAAC 
TTCCCACTCA ACATGGGGCC TATCCCAGCC CCGTACGTGC CCCCTCTGCC CAACGTGCGG 
GTCAACTATG ACTTCGGTCC CATCCACATG CCCCTGGAGC ACAACCTGCC CATGCACTTT 
GGCCCCCAGC CGCGGCATCG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_011515436.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513738.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu41206
Clone ID Related Accession (Same CDS sequence)	XM_017012365.1 , XM_011515436.1 , XM_024446806.1 , XM_011515436.2 , XM_024446807.1
Accession Version	XM_011515436.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGACT TCAAAGTGCT CAGTAGTCAG GACATCAAGT GGGCCCTGCA CGAGCTCAAA 
GGACACTATG CAATCACCCG AAAGGCCTTG TCTGATGCCA TTAAAAAATG GCAGGAGCTG 
TCACCAGAAA CCAGTGGAAA AAGGAAGAAG AGAAAACAAA TGAACCAGTA TTCTTACATT 
GATTTCAAGT TTGAACAAGG TGACATAAAA ATAGAAAAGA GGATGTTCTT TCTTGAAAAT 
AAGCGACGAC ATTGTAGGTC CTATGACCGA CGTGCTCTCC TTCCAGCTGT GCAACAAGAG 
CAGGAGTTCT ATGAGCAGAA AATCAAAGAG ATGGCAGAGC ATGAAGACTT TTTGCTTGCC 
CTACAGATGA ATGAAGAACA GTATCAAAAG GATGGCCAGC TGATTGAGTG TCGCTGCTGC 
TATGGGGAAT TTCCATTCGA GGAGCTGACG CAGTGCGCAG ATGCTCACTT GTTCTGCAAA 
GAGTGTCTCA TCAGATATGC CCAAGAGGCA GTCTTTGGAT CTGGAAAGTT GGAGCTCAGC 
TGCATGGAAG GCAGCTGCAC GTGTTCGTTC CCAACCAGTG AGCTGGAGAA GGTGCTCCCC 
CAGACCATCC TGTATAAGTA CTATGAGCGA AAAGCCGAGG AGGAGGTTGC GGCAGCCTAC 
GCCGACGAGC TTGTCAGGTG CCCGTCCTGT AGCTTTCCGG CTCTGTTGGA CAGTGATGTG 
AAGAGGTTCA GCTGTCCTAA TCCTCACTGC CGAAAGGAAA CCTGTAGGAA GTGTCAGGGA 
CTCTGGAAAG AACATAATGG CCTCACCTGT GAAGAGCTGG CTGAAAAAGA CGACATCAAG 
TACCGTACCT CTATTGAAGA AAAAATGACT GCTGCCCGCA TTAGAAAATG CCACAAGTGT 
GGGACTGGCC TCATCAAATC TGAAGGCTGC AACCGCATGT CTTGCCGCTG TGGTGCCCAG 
ATGTGCTACC TCTGTCGAGT TTCTATTAAT GGATATGACC ATTTCTGCCA ACATCCCCGC 
TCACCAGGAG CCCCTTGCCA GGAGTGTTCA AGATGCTCTC TCTGGACCGA TCCCACTGAA 
GATGATGAGA AGCTTATTGA GGAAATCCAG AAGGAGGCTG AAGAGGAACA GAAAAGAAAG 
AATGGAGAGA ACACCTTCAA ACGCATTGGA CCCCCGCTGG AGAAGCCTGT GGAGAAGGTG 
CAGAGGGTGG AGGCCCTCCC GAGGCCCGTT CCGCAGAACC TGCCACAGCC ACAGATGCCA 
CCCTATGCCT TCGCGCACCC ACCCTTCCCC CTGCCTCCCG TGCGGCCTGT GTTCAACAAC 
TTCCCACTCA ACATGGGGCC TATCCCAGCC CCGTACGTGC CCCCTCTGCC CAACGTGCGG 
GTCAACTATG ACTTCGGTCC CATCCACATG CCCCTGGAGC ACAACCTGCC CATGCACTTT 
GGCCCCCAGC CGCGGCATCG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513738.1
CDS	1304..2770
Translation	MADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_011515436.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515436.1

ORF Insert Sequence:

ATGGCCGACT TCAAAGTGCT CAGTAGTCAG GACATCAAGT GGGCCCTGCA CGAGCTCAAA 
GGACACTATG CAATCACCCG AAAGGCCTTG TCTGATGCCA TTAAAAAATG GCAGGAGCTG 
TCACCAGAAA CCAGTGGAAA AAGGAAGAAG AGAAAACAAA TGAACCAGTA TTCTTACATT 
GATTTCAAGT TTGAACAAGG TGACATAAAA ATAGAAAAGA GGATGTTCTT TCTTGAAAAT 
AAGCGACGAC ATTGTAGGTC CTATGACCGA CGTGCTCTCC TTCCAGCTGT GCAACAAGAG 
CAGGAGTTCT ATGAGCAGAA AATCAAAGAG ATGGCAGAGC ATGAAGACTT TTTGCTTGCC 
CTACAGATGA ATGAAGAACA GTATCAAAAG GATGGCCAGC TGATTGAGTG TCGCTGCTGC 
TATGGGGAAT TTCCATTCGA GGAGCTGACG CAGTGCGCAG ATGCTCACTT GTTCTGCAAA 
GAGTGTCTCA TCAGATATGC CCAAGAGGCA GTCTTTGGAT CTGGAAAGTT GGAGCTCAGC 
TGCATGGAAG GCAGCTGCAC GTGTTCGTTC CCAACCAGTG AGCTGGAGAA GGTGCTCCCC 
CAGACCATCC TGTATAAGTA CTATGAGCGA AAAGCCGAGG AGGAGGTTGC GGCAGCCTAC 
GCCGACGAGC TTGTCAGGTG CCCGTCCTGT AGCTTTCCGG CTCTGTTGGA CAGTGATGTG 
AAGAGGTTCA GCTGTCCTAA TCCTCACTGC CGAAAGGAAA CCTGTAGGAA GTGTCAGGGA 
CTCTGGAAAG AACATAATGG CCTCACCTGT GAAGAGCTGG CTGAAAAAGA CGACATCAAG 
TACCGTACCT CTATTGAAGA AAAAATGACT GCTGCCCGCA TTAGAAAATG CCACAAGTGT 
GGGACTGGCC TCATCAAATC TGAAGGCTGC AACCGCATGT CTTGCCGCTG TGGTGCCCAG 
ATGTGCTACC TCTGTCGAGT TTCTATTAAT GGATATGACC ATTTCTGCCA ACATCCCCGC 
TCACCAGGAG CCCCTTGCCA GGAGTGTTCA AGATGCTCTC TCTGGACCGA TCCCACTGAA 
GATGATGAGA AGCTTATTGA GGAAATCCAG AAGGAGGCTG AAGAGGAACA GAAAAGAAAG 
AATGGAGAGA ACACCTTCAA ACGCATTGGA CCCCCGCTGG AGAAGCCTGT GGAGAAGGTG 
CAGAGGGTGG AGGCCCTCCC GAGGCCCGTT CCGCAGAACC TGCCACAGCC ACAGATGCCA 
CCCTATGCCT TCGCGCACCC ACCCTTCCCC CTGCCTCCCG TGCGGCCTGT GTTCAACAAC 
TTCCCACTCA ACATGGGGCC TATCCCAGCC CCGTACGTGC CCCCTCTGCC CAACGTGCGG 
GTCAACTATG ACTTCGGTCC CATCCACATG CCCCTGGAGC ACAACCTGCC CATGCACTTT 
GGCCCCCAGC CGCGGCATCG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_024446806.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302574.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu41206
Clone ID Related Accession (Same CDS sequence)	XM_017012365.1 , XM_011515436.1 , XM_024446806.1 , XM_011515436.2 , XM_024446807.1
Accession Version	XM_024446806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGACT TCAAAGTGCT CAGTAGTCAG GACATCAAGT GGGCCCTGCA CGAGCTCAAA 
GGACACTATG CAATCACCCG AAAGGCCTTG TCTGATGCCA TTAAAAAATG GCAGGAGCTG 
TCACCAGAAA CCAGTGGAAA AAGGAAGAAG AGAAAACAAA TGAACCAGTA TTCTTACATT 
GATTTCAAGT TTGAACAAGG TGACATAAAA ATAGAAAAGA GGATGTTCTT TCTTGAAAAT 
AAGCGACGAC ATTGTAGGTC CTATGACCGA CGTGCTCTCC TTCCAGCTGT GCAACAAGAG 
CAGGAGTTCT ATGAGCAGAA AATCAAAGAG ATGGCAGAGC ATGAAGACTT TTTGCTTGCC 
CTACAGATGA ATGAAGAACA GTATCAAAAG GATGGCCAGC TGATTGAGTG TCGCTGCTGC 
TATGGGGAAT TTCCATTCGA GGAGCTGACG CAGTGCGCAG ATGCTCACTT GTTCTGCAAA 
GAGTGTCTCA TCAGATATGC CCAAGAGGCA GTCTTTGGAT CTGGAAAGTT GGAGCTCAGC 
TGCATGGAAG GCAGCTGCAC GTGTTCGTTC CCAACCAGTG AGCTGGAGAA GGTGCTCCCC 
CAGACCATCC TGTATAAGTA CTATGAGCGA AAAGCCGAGG AGGAGGTTGC GGCAGCCTAC 
GCCGACGAGC TTGTCAGGTG CCCGTCCTGT AGCTTTCCGG CTCTGTTGGA CAGTGATGTG 
AAGAGGTTCA GCTGTCCTAA TCCTCACTGC CGAAAGGAAA CCTGTAGGAA GTGTCAGGGA 
CTCTGGAAAG AACATAATGG CCTCACCTGT GAAGAGCTGG CTGAAAAAGA CGACATCAAG 
TACCGTACCT CTATTGAAGA AAAAATGACT GCTGCCCGCA TTAGAAAATG CCACAAGTGT 
GGGACTGGCC TCATCAAATC TGAAGGCTGC AACCGCATGT CTTGCCGCTG TGGTGCCCAG 
ATGTGCTACC TCTGTCGAGT TTCTATTAAT GGATATGACC ATTTCTGCCA ACATCCCCGC 
TCACCAGGAG CCCCTTGCCA GGAGTGTTCA AGATGCTCTC TCTGGACCGA TCCCACTGAA 
GATGATGAGA AGCTTATTGA GGAAATCCAG AAGGAGGCTG AAGAGGAACA GAAAAGAAAG 
AATGGAGAGA ACACCTTCAA ACGCATTGGA CCCCCGCTGG AGAAGCCTGT GGAGAAGGTG 
CAGAGGGTGG AGGCCCTCCC GAGGCCCGTT CCGCAGAACC TGCCACAGCC ACAGATGCCA 
CCCTATGCCT TCGCGCACCC ACCCTTCCCC CTGCCTCCCG TGCGGCCTGT GTTCAACAAC 
TTCCCACTCA ACATGGGGCC TATCCCAGCC CCGTACGTGC CCCCTCTGCC CAACGTGCGG 
GTCAACTATG ACTTCGGTCC CATCCACATG CCCCTGGAGC ACAACCTGCC CATGCACTTT 
GGCCCCCAGC CGCGGCATCG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302574.1
CDS	1766..3232
Translation	MADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_024446806.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446806.1

ORF Insert Sequence:

ATGGCCGACT TCAAAGTGCT CAGTAGTCAG GACATCAAGT GGGCCCTGCA CGAGCTCAAA 
GGACACTATG CAATCACCCG AAAGGCCTTG TCTGATGCCA TTAAAAAATG GCAGGAGCTG 
TCACCAGAAA CCAGTGGAAA AAGGAAGAAG AGAAAACAAA TGAACCAGTA TTCTTACATT 
GATTTCAAGT TTGAACAAGG TGACATAAAA ATAGAAAAGA GGATGTTCTT TCTTGAAAAT 
AAGCGACGAC ATTGTAGGTC CTATGACCGA CGTGCTCTCC TTCCAGCTGT GCAACAAGAG 
CAGGAGTTCT ATGAGCAGAA AATCAAAGAG ATGGCAGAGC ATGAAGACTT TTTGCTTGCC 
CTACAGATGA ATGAAGAACA GTATCAAAAG GATGGCCAGC TGATTGAGTG TCGCTGCTGC 
TATGGGGAAT TTCCATTCGA GGAGCTGACG CAGTGCGCAG ATGCTCACTT GTTCTGCAAA 
GAGTGTCTCA TCAGATATGC CCAAGAGGCA GTCTTTGGAT CTGGAAAGTT GGAGCTCAGC 
TGCATGGAAG GCAGCTGCAC GTGTTCGTTC CCAACCAGTG AGCTGGAGAA GGTGCTCCCC 
CAGACCATCC TGTATAAGTA CTATGAGCGA AAAGCCGAGG AGGAGGTTGC GGCAGCCTAC 
GCCGACGAGC TTGTCAGGTG CCCGTCCTGT AGCTTTCCGG CTCTGTTGGA CAGTGATGTG 
AAGAGGTTCA GCTGTCCTAA TCCTCACTGC CGAAAGGAAA CCTGTAGGAA GTGTCAGGGA 
CTCTGGAAAG AACATAATGG CCTCACCTGT GAAGAGCTGG CTGAAAAAGA CGACATCAAG 
TACCGTACCT CTATTGAAGA AAAAATGACT GCTGCCCGCA TTAGAAAATG CCACAAGTGT 
GGGACTGGCC TCATCAAATC TGAAGGCTGC AACCGCATGT CTTGCCGCTG TGGTGCCCAG 
ATGTGCTACC TCTGTCGAGT TTCTATTAAT GGATATGACC ATTTCTGCCA ACATCCCCGC 
TCACCAGGAG CCCCTTGCCA GGAGTGTTCA AGATGCTCTC TCTGGACCGA TCCCACTGAA 
GATGATGAGA AGCTTATTGA GGAAATCCAG AAGGAGGCTG AAGAGGAACA GAAAAGAAAG 
AATGGAGAGA ACACCTTCAA ACGCATTGGA CCCCCGCTGG AGAAGCCTGT GGAGAAGGTG 
CAGAGGGTGG AGGCCCTCCC GAGGCCCGTT CCGCAGAACC TGCCACAGCC ACAGATGCCA 
CCCTATGCCT TCGCGCACCC ACCCTTCCCC CTGCCTCCCG TGCGGCCTGT GTTCAACAAC 
TTCCCACTCA ACATGGGGCC TATCCCAGCC CCGTACGTGC CCCCTCTGCC CAACGTGCGG 
GTCAACTATG ACTTCGGTCC CATCCACATG CCCCTGGAGC ACAACCTGCC CATGCACTTT 
GGCCCCCAGC CGCGGCATCG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_011515436.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513738.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant X7, mRNA.

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CloneID	OHu41206
Clone ID Related Accession (Same CDS sequence)	XM_017012365.1 , XM_011515436.1 , XM_024446806.1 , XM_011515436.2 , XM_024446807.1
Accession Version	XM_011515436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515436.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGACT TCAAAGTGCT CAGTAGTCAG GACATCAAGT GGGCCCTGCA CGAGCTCAAA 
GGACACTATG CAATCACCCG AAAGGCCTTG TCTGATGCCA TTAAAAAATG GCAGGAGCTG 
TCACCAGAAA CCAGTGGAAA AAGGAAGAAG AGAAAACAAA TGAACCAGTA TTCTTACATT 
GATTTCAAGT TTGAACAAGG TGACATAAAA ATAGAAAAGA GGATGTTCTT TCTTGAAAAT 
AAGCGACGAC ATTGTAGGTC CTATGACCGA CGTGCTCTCC TTCCAGCTGT GCAACAAGAG 
CAGGAGTTCT ATGAGCAGAA AATCAAAGAG ATGGCAGAGC ATGAAGACTT TTTGCTTGCC 
CTACAGATGA ATGAAGAACA GTATCAAAAG GATGGCCAGC TGATTGAGTG TCGCTGCTGC 
TATGGGGAAT TTCCATTCGA GGAGCTGACG CAGTGCGCAG ATGCTCACTT GTTCTGCAAA 
GAGTGTCTCA TCAGATATGC CCAAGAGGCA GTCTTTGGAT CTGGAAAGTT GGAGCTCAGC 
TGCATGGAAG GCAGCTGCAC GTGTTCGTTC CCAACCAGTG AGCTGGAGAA GGTGCTCCCC 
CAGACCATCC TGTATAAGTA CTATGAGCGA AAAGCCGAGG AGGAGGTTGC GGCAGCCTAC 
GCCGACGAGC TTGTCAGGTG CCCGTCCTGT AGCTTTCCGG CTCTGTTGGA CAGTGATGTG 
AAGAGGTTCA GCTGTCCTAA TCCTCACTGC CGAAAGGAAA CCTGTAGGAA GTGTCAGGGA 
CTCTGGAAAG AACATAATGG CCTCACCTGT GAAGAGCTGG CTGAAAAAGA CGACATCAAG 
TACCGTACCT CTATTGAAGA AAAAATGACT GCTGCCCGCA TTAGAAAATG CCACAAGTGT 
GGGACTGGCC TCATCAAATC TGAAGGCTGC AACCGCATGT CTTGCCGCTG TGGTGCCCAG 
ATGTGCTACC TCTGTCGAGT TTCTATTAAT GGATATGACC ATTTCTGCCA ACATCCCCGC 
TCACCAGGAG CCCCTTGCCA GGAGTGTTCA AGATGCTCTC TCTGGACCGA TCCCACTGAA 
GATGATGAGA AGCTTATTGA GGAAATCCAG AAGGAGGCTG AAGAGGAACA GAAAAGAAAG 
AATGGAGAGA ACACCTTCAA ACGCATTGGA CCCCCGCTGG AGAAGCCTGT GGAGAAGGTG 
CAGAGGGTGG AGGCCCTCCC GAGGCCCGTT CCGCAGAACC TGCCACAGCC ACAGATGCCA 
CCCTATGCCT TCGCGCACCC ACCCTTCCCC CTGCCTCCCG TGCGGCCTGT GTTCAACAAC 
TTCCCACTCA ACATGGGGCC TATCCCAGCC CCGTACGTGC CCCCTCTGCC CAACGTGCGG 
GTCAACTATG ACTTCGGTCC CATCCACATG CCCCTGGAGC ACAACCTGCC CATGCACTTT 
GGCCCCCAGC CGCGGCATCG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513738.1
CDS	1591..3057
Translation	MADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_011515436.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515436.2

ORF Insert Sequence:

ATGGCCGACT TCAAAGTGCT CAGTAGTCAG GACATCAAGT GGGCCCTGCA CGAGCTCAAA 
GGACACTATG CAATCACCCG AAAGGCCTTG TCTGATGCCA TTAAAAAATG GCAGGAGCTG 
TCACCAGAAA CCAGTGGAAA AAGGAAGAAG AGAAAACAAA TGAACCAGTA TTCTTACATT 
GATTTCAAGT TTGAACAAGG TGACATAAAA ATAGAAAAGA GGATGTTCTT TCTTGAAAAT 
AAGCGACGAC ATTGTAGGTC CTATGACCGA CGTGCTCTCC TTCCAGCTGT GCAACAAGAG 
CAGGAGTTCT ATGAGCAGAA AATCAAAGAG ATGGCAGAGC ATGAAGACTT TTTGCTTGCC 
CTACAGATGA ATGAAGAACA GTATCAAAAG GATGGCCAGC TGATTGAGTG TCGCTGCTGC 
TATGGGGAAT TTCCATTCGA GGAGCTGACG CAGTGCGCAG ATGCTCACTT GTTCTGCAAA 
GAGTGTCTCA TCAGATATGC CCAAGAGGCA GTCTTTGGAT CTGGAAAGTT GGAGCTCAGC 
TGCATGGAAG GCAGCTGCAC GTGTTCGTTC CCAACCAGTG AGCTGGAGAA GGTGCTCCCC 
CAGACCATCC TGTATAAGTA CTATGAGCGA AAAGCCGAGG AGGAGGTTGC GGCAGCCTAC 
GCCGACGAGC TTGTCAGGTG CCCGTCCTGT AGCTTTCCGG CTCTGTTGGA CAGTGATGTG 
AAGAGGTTCA GCTGTCCTAA TCCTCACTGC CGAAAGGAAA CCTGTAGGAA GTGTCAGGGA 
CTCTGGAAAG AACATAATGG CCTCACCTGT GAAGAGCTGG CTGAAAAAGA CGACATCAAG 
TACCGTACCT CTATTGAAGA AAAAATGACT GCTGCCCGCA TTAGAAAATG CCACAAGTGT 
GGGACTGGCC TCATCAAATC TGAAGGCTGC AACCGCATGT CTTGCCGCTG TGGTGCCCAG 
ATGTGCTACC TCTGTCGAGT TTCTATTAAT GGATATGACC ATTTCTGCCA ACATCCCCGC 
TCACCAGGAG CCCCTTGCCA GGAGTGTTCA AGATGCTCTC TCTGGACCGA TCCCACTGAA 
GATGATGAGA AGCTTATTGA GGAAATCCAG AAGGAGGCTG AAGAGGAACA GAAAAGAAAG 
AATGGAGAGA ACACCTTCAA ACGCATTGGA CCCCCGCTGG AGAAGCCTGT GGAGAAGGTG 
CAGAGGGTGG AGGCCCTCCC GAGGCCCGTT CCGCAGAACC TGCCACAGCC ACAGATGCCA 
CCCTATGCCT TCGCGCACCC ACCCTTCCCC CTGCCTCCCG TGCGGCCTGT GTTCAACAAC 
TTCCCACTCA ACATGGGGCC TATCCCAGCC CCGTACGTGC CCCCTCTGCC CAACGTGCGG 
GTCAACTATG ACTTCGGTCC CATCCACATG CCCCTGGAGC ACAACCTGCC CATGCACTTT 
GGCCCCCAGC CGCGGCATCG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_024446807.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302575.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant X6, mRNA.

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CloneID	OHu41206
Clone ID Related Accession (Same CDS sequence)	XM_017012365.1 , XM_011515436.1 , XM_024446806.1 , XM_011515436.2 , XM_024446807.1
Accession Version	XM_024446807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGACT TCAAAGTGCT CAGTAGTCAG GACATCAAGT GGGCCCTGCA CGAGCTCAAA 
GGACACTATG CAATCACCCG AAAGGCCTTG TCTGATGCCA TTAAAAAATG GCAGGAGCTG 
TCACCAGAAA CCAGTGGAAA AAGGAAGAAG AGAAAACAAA TGAACCAGTA TTCTTACATT 
GATTTCAAGT TTGAACAAGG TGACATAAAA ATAGAAAAGA GGATGTTCTT TCTTGAAAAT 
AAGCGACGAC ATTGTAGGTC CTATGACCGA CGTGCTCTCC TTCCAGCTGT GCAACAAGAG 
CAGGAGTTCT ATGAGCAGAA AATCAAAGAG ATGGCAGAGC ATGAAGACTT TTTGCTTGCC 
CTACAGATGA ATGAAGAACA GTATCAAAAG GATGGCCAGC TGATTGAGTG TCGCTGCTGC 
TATGGGGAAT TTCCATTCGA GGAGCTGACG CAGTGCGCAG ATGCTCACTT GTTCTGCAAA 
GAGTGTCTCA TCAGATATGC CCAAGAGGCA GTCTTTGGAT CTGGAAAGTT GGAGCTCAGC 
TGCATGGAAG GCAGCTGCAC GTGTTCGTTC CCAACCAGTG AGCTGGAGAA GGTGCTCCCC 
CAGACCATCC TGTATAAGTA CTATGAGCGA AAAGCCGAGG AGGAGGTTGC GGCAGCCTAC 
GCCGACGAGC TTGTCAGGTG CCCGTCCTGT AGCTTTCCGG CTCTGTTGGA CAGTGATGTG 
AAGAGGTTCA GCTGTCCTAA TCCTCACTGC CGAAAGGAAA CCTGTAGGAA GTGTCAGGGA 
CTCTGGAAAG AACATAATGG CCTCACCTGT GAAGAGCTGG CTGAAAAAGA CGACATCAAG 
TACCGTACCT CTATTGAAGA AAAAATGACT GCTGCCCGCA TTAGAAAATG CCACAAGTGT 
GGGACTGGCC TCATCAAATC TGAAGGCTGC AACCGCATGT CTTGCCGCTG TGGTGCCCAG 
ATGTGCTACC TCTGTCGAGT TTCTATTAAT GGATATGACC ATTTCTGCCA ACATCCCCGC 
TCACCAGGAG CCCCTTGCCA GGAGTGTTCA AGATGCTCTC TCTGGACCGA TCCCACTGAA 
GATGATGAGA AGCTTATTGA GGAAATCCAG AAGGAGGCTG AAGAGGAACA GAAAAGAAAG 
AATGGAGAGA ACACCTTCAA ACGCATTGGA CCCCCGCTGG AGAAGCCTGT GGAGAAGGTG 
CAGAGGGTGG AGGCCCTCCC GAGGCCCGTT CCGCAGAACC TGCCACAGCC ACAGATGCCA 
CCCTATGCCT TCGCGCACCC ACCCTTCCCC CTGCCTCCCG TGCGGCCTGT GTTCAACAAC 
TTCCCACTCA ACATGGGGCC TATCCCAGCC CCGTACGTGC CCCCTCTGCC CAACGTGCGG 
GTCAACTATG ACTTCGGTCC CATCCACATG CCCCTGGAGC ACAACCTGCC CATGCACTTT 
GGCCCCCAGC CGCGGCATCG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302575.1
CDS	1623..3089
Translation	MADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_024446807.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446807.1

ORF Insert Sequence:

ATGGCCGACT TCAAAGTGCT CAGTAGTCAG GACATCAAGT GGGCCCTGCA CGAGCTCAAA 
GGACACTATG CAATCACCCG AAAGGCCTTG TCTGATGCCA TTAAAAAATG GCAGGAGCTG 
TCACCAGAAA CCAGTGGAAA AAGGAAGAAG AGAAAACAAA TGAACCAGTA TTCTTACATT 
GATTTCAAGT TTGAACAAGG TGACATAAAA ATAGAAAAGA GGATGTTCTT TCTTGAAAAT 
AAGCGACGAC ATTGTAGGTC CTATGACCGA CGTGCTCTCC TTCCAGCTGT GCAACAAGAG 
CAGGAGTTCT ATGAGCAGAA AATCAAAGAG ATGGCAGAGC ATGAAGACTT TTTGCTTGCC 
CTACAGATGA ATGAAGAACA GTATCAAAAG GATGGCCAGC TGATTGAGTG TCGCTGCTGC 
TATGGGGAAT TTCCATTCGA GGAGCTGACG CAGTGCGCAG ATGCTCACTT GTTCTGCAAA 
GAGTGTCTCA TCAGATATGC CCAAGAGGCA GTCTTTGGAT CTGGAAAGTT GGAGCTCAGC 
TGCATGGAAG GCAGCTGCAC GTGTTCGTTC CCAACCAGTG AGCTGGAGAA GGTGCTCCCC 
CAGACCATCC TGTATAAGTA CTATGAGCGA AAAGCCGAGG AGGAGGTTGC GGCAGCCTAC 
GCCGACGAGC TTGTCAGGTG CCCGTCCTGT AGCTTTCCGG CTCTGTTGGA CAGTGATGTG 
AAGAGGTTCA GCTGTCCTAA TCCTCACTGC CGAAAGGAAA CCTGTAGGAA GTGTCAGGGA 
CTCTGGAAAG AACATAATGG CCTCACCTGT GAAGAGCTGG CTGAAAAAGA CGACATCAAG 
TACCGTACCT CTATTGAAGA AAAAATGACT GCTGCCCGCA TTAGAAAATG CCACAAGTGT 
GGGACTGGCC TCATCAAATC TGAAGGCTGC AACCGCATGT CTTGCCGCTG TGGTGCCCAG 
ATGTGCTACC TCTGTCGAGT TTCTATTAAT GGATATGACC ATTTCTGCCA ACATCCCCGC 
TCACCAGGAG CCCCTTGCCA GGAGTGTTCA AGATGCTCTC TCTGGACCGA TCCCACTGAA 
GATGATGAGA AGCTTATTGA GGAAATCCAG AAGGAGGCTG AAGAGGAACA GAAAAGAAAG 
AATGGAGAGA ACACCTTCAA ACGCATTGGA CCCCCGCTGG AGAAGCCTGT GGAGAAGGTG 
CAGAGGGTGG AGGCCCTCCC GAGGCCCGTT CCGCAGAACC TGCCACAGCC ACAGATGCCA 
CCCTATGCCT TCGCGCACCC ACCCTTCCCC CTGCCTCCCG TGCGGCCTGT GTTCAACAAC 
TTCCCACTCA ACATGGGGCC TATCCCAGCC CCGTACGTGC CCCCTCTGCC CAACGTGCGG 
GTCAACTATG ACTTCGGTCC CATCCACATG CCCCTGGAGC ACAACCTGCC CATGCACTTT 
GGCCCCCAGC CGCGGCATCG CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( NM_207111.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_996994.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant 1, mRNA.

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CloneID	OHu22477
Clone ID Related Accession (Same CDS sequence)	NM_207111.3 , XM_005249785.2 , NM_207111.4 , XM_024446805.1
Accession Version	NM_207111.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-20
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292396.1, AF513718.1, AC006483.3 and BM970811.1. This sequence is a reference standard in the RefSeqGene project. On Jan 21, 2011 this sequence version replaced NM_207111.2. Transcript Variant: This variant (1, also known as TRIAD3B) represents the longer transcript and it encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.144372.1, SRR1803615.164842.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AACAAATAAA CCTCAGAGAT CACGACCCAA TCTCATCAAA 
CCAGCTGCCC AGTGGCAAGA TCTGAAAAGG TTGGGAGAAG AAAGGCCTAA AAAGTCTAGA 
GCAGCATTTG AATCAGATAA GAGCAGCTAT TTTTCAGTGT GTAACAACCC ATTGTTTGAT 
TCTGGGGCAC AGGATGATTC TGAGGATGAC TACGGTGAAT TTCTGGATCT TGGGCCTCCT 
GGAATCTCTG AATTCACTAA GCCAAGTGGC CAAACAGAAA GAGAACCCAA GCCTGGACCG 
AGTCATAACC AAGCAGCAAA TGACATTGTC AACCCCAGAT CAGAGCAGAA AGTCATCATC 
TTGGAAGAAG GTAGCCTTCT TTACACAGAA AGCGATCCTT TGGAAACTCA GAACCAGTCA 
TCCGAAGACT CAGAGACAGA GCTGTTATCA AATCTAGGAG AGTCAGCTGC TCTAGCAGAT 
GATCAGGCCA TCGAAGAAGA CTGCTGGTTA GATCATCCTT ACTTCCAGTC TCTGAACCAA 
CAGCCCCGTG AAATAACAAA CCAGGTCGTT CCTCAGGAAC GGCAGCCTGA AGCAGAACTG 
GGCCGCTTGT TGTTTCAGCA TGAATTCCCA GGGCCCGCTT TTCCAAGGCC GGAACCCCAG 
CAAGGTGGGA TTTCAGGCCC CTCTTCTCCT CAGCCTGCCC ATCCTCTAGG AGAGTTTGAA 
GACCAGCAGT TAGCAAGTGA TGATGAAGAG CCAGGTCCAG CCTTTCCAAT GCAAGAATCT 
CAAGAGCCCA ATTTGGAAAA CATTTGGGGG CAAGAAGCTG CAGAGGTAGA TCAAGAGCTC 
GTTGAACTAC TAGTGAAAGA AACGGAAGCA AGATTTCCAG ATGTAGCAAA TGGGTTTATT 
GAGGAAATAA TTCATTTTAA GAATTATTAT GATCTGAATG TACTTTGTAA TTTTCTTCTG 
GAAAACCCAG ATTATCCAAA GAGAGAAGAC AGAATCATTA TAAATCCCAG TAGCAGTCTG 
CTGGCCAGCC AAGATGAGAC AAAGTTGCCT AAAATAGACT TTTTTGACTA TTCTAAATTG 
ACCCCTCTTG ACCAGCGCTG CTTCATCCAA GCTGCTGACC TCCTCATGGC CGACTTCAAA 
GTGCTCAGTA GTCAGGACAT CAAGTGGGCC CTGCACGAGC TCAAAGGACA CTATGCAATC 
ACCCGAAAGG CCTTGTCTGA TGCCATTAAA AAATGGCAGG AGCTGTCACC AGAAACCAGT 
GGAAAAAGGA AGAAGAGAAA ACAAATGAAC CAGTATTCTT ACATTGATTT CAAGTTTGAA 
CAAGGTGACA TAAAAATAGA AAAGAGGATG TTCTTTCTTG AAAATAAGCG ACGACATTGT 
AGGTCCTATG ACCGACGTGC TCTCCTTCCA GCTGTGCAAC AAGAGCAGGA GTTCTATGAG 
CAGAAAATCA AAGAGATGGC AGAGCATGAA GACTTTTTGC TTGCCCTACA GATGAATGAA 
GAACAGTATC AAAAGGATGG CCAGCTGATT GAGTGTCGCT GCTGCTATGG GGAATTTCCA 
TTCGAGGAGC TGACGCAGTG CGCAGATGCT CACTTGTTCT GCAAAGAGTG TCTCATCAGA 
TATGCCCAAG AGGCAGTCTT TGGATCTGGA AAGTTGGAGC TCAGCTGCAT GGAAGGCAGC 
TGCACGTGTT CGTTCCCAAC CAGTGAGCTG GAGAAGGTGC TCCCCCAGAC CATCCTGTAT 
AAGTACTATG AGCGAAAAGC CGAGGAGGAG GTTGCGGCAG CCTACGCCGA CGAGCTTGTC 
AGGTGCCCGT CCTGTAGCTT TCCGGCTCTG TTGGACAGTG ATGTGAAGAG GTTCAGCTGT 
CCTAATCCTC ACTGCCGAAA GGAAACCTGT AGGAAGTGTC AGGGACTCTG GAAAGAACAT 
AATGGCCTCA CCTGTGAAGA GCTGGCTGAA AAAGACGACA TCAAGTACCG TACCTCTATT 
GAAGAAAAAA TGACTGCTGC CCGCATTAGA AAATGCCACA AGTGTGGGAC TGGCCTCATC 
AAATCTGAAG GCTGCAACCG CATGTCTTGC CGCTGTGGTG CCCAGATGTG CTACCTCTGT 
CGAGTTTCTA TTAATGGATA TGACCATTTC TGCCAACATC CCCGCTCACC AGGAGCCCCT 
TGCCAGGAGT GTTCAAGATG CTCTCTCTGG ACCGATCCCA CTGAAGATGA TGAGAAGCTT 
ATTGAGGAAA TCCAGAAGGA GGCTGAAGAG GAACAGAAAA GAAAGAATGG AGAGAACACC 
TTCAAACGCA TTGGACCCCC GCTGGAGAAG CCTGTGGAGA AGGTGCAGAG GGTGGAGGCC 
CTCCCGAGGC CCGTTCCGCA GAACCTGCCA CAGCCACAGA TGCCACCCTA TGCCTTCGCG 
CACCCACCCT TCCCCCTGCC TCCCGTGCGG CCTGTGTTCA ACAACTTCCC ACTCAACATG 
GGGCCTATCC CAGCCCCGTA CGTGCCCCCT CTGCCCAACG TGCGGGTCAA CTATGACTTC 
GGTCCCATCC ACATGCCCCT GGAGCACAAC CTGCCCATGC ACTTTGGCCC CCAGCCGCGG 
CATCGCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996994.1
CDS	260..3031
Translation	MEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIPMLVTPAPQQHEEEDLDDDVILTETNKPQRSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDKSSYFSVCNNPLFDSGAQDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGPSHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAALADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPRPEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAAEVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRIIINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	NM_207111.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207111.3

ORF Insert Sequence:

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AACAAATAAA CCTCAGAGAT CACGACCCAA TCTCATCAAA 
CCAGCTGCCC AGTGGCAAGA TCTGAAAAGG TTGGGAGAAG AAAGGCCTAA AAAGTCTAGA 
GCAGCATTTG AATCAGATAA GAGCAGCTAT TTTTCAGTGT GTAACAACCC ATTGTTTGAT 
TCTGGGGCAC AGGATGATTC TGAGGATGAC TACGGTGAAT TTCTGGATCT TGGGCCTCCT 
GGAATCTCTG AATTCACTAA GCCAAGTGGC CAAACAGAAA GAGAACCCAA GCCTGGACCG 
AGTCATAACC AAGCAGCAAA TGACATTGTC AACCCCAGAT CAGAGCAGAA AGTCATCATC 
TTGGAAGAAG GTAGCCTTCT TTACACAGAA AGCGATCCTT TGGAAACTCA GAACCAGTCA 
TCCGAAGACT CAGAGACAGA GCTGTTATCA AATCTAGGAG AGTCAGCTGC TCTAGCAGAT 
GATCAGGCCA TCGAAGAAGA CTGCTGGTTA GATCATCCTT ACTTCCAGTC TCTGAACCAA 
CAGCCCCGTG AAATAACAAA CCAGGTCGTT CCTCAGGAAC GGCAGCCTGA AGCAGAACTG 
GGCCGCTTGT TGTTTCAGCA TGAATTCCCA GGGCCCGCTT TTCCAAGGCC GGAACCCCAG 
CAAGGTGGGA TTTCAGGCCC CTCTTCTCCT CAGCCTGCCC ATCCTCTAGG AGAGTTTGAA 
GACCAGCAGT TAGCAAGTGA TGATGAAGAG CCAGGTCCAG CCTTTCCAAT GCAAGAATCT 
CAAGAGCCCA ATTTGGAAAA CATTTGGGGG CAAGAAGCTG CAGAGGTAGA TCAAGAGCTC 
GTTGAACTAC TAGTGAAAGA AACGGAAGCA AGATTTCCAG ATGTAGCAAA TGGGTTTATT 
GAGGAAATAA TTCATTTTAA GAATTATTAT GATCTGAATG TACTTTGTAA TTTTCTTCTG 
GAAAACCCAG ATTATCCAAA GAGAGAAGAC AGAATCATTA TAAATCCCAG TAGCAGTCTG 
CTGGCCAGCC AAGATGAGAC AAAGTTGCCT AAAATAGACT TTTTTGACTA TTCTAAATTG 
ACCCCTCTTG ACCAGCGCTG CTTCATCCAA GCTGCTGACC TCCTCATGGC CGACTTCAAA 
GTGCTCAGTA GTCAGGACAT CAAGTGGGCC CTGCACGAGC TCAAAGGACA CTATGCAATC 
ACCCGAAAGG CCTTGTCTGA TGCCATTAAA AAATGGCAGG AGCTGTCACC AGAAACCAGT 
GGAAAAAGGA AGAAGAGAAA ACAAATGAAC CAGTATTCTT ACATTGATTT CAAGTTTGAA 
CAAGGTGACA TAAAAATAGA AAAGAGGATG TTCTTTCTTG AAAATAAGCG ACGACATTGT 
AGGTCCTATG ACCGACGTGC TCTCCTTCCA GCTGTGCAAC AAGAGCAGGA GTTCTATGAG 
CAGAAAATCA AAGAGATGGC AGAGCATGAA GACTTTTTGC TTGCCCTACA GATGAATGAA 
GAACAGTATC AAAAGGATGG CCAGCTGATT GAGTGTCGCT GCTGCTATGG GGAATTTCCA 
TTCGAGGAGC TGACGCAGTG CGCAGATGCT CACTTGTTCT GCAAAGAGTG TCTCATCAGA 
TATGCCCAAG AGGCAGTCTT TGGATCTGGA AAGTTGGAGC TCAGCTGCAT GGAAGGCAGC 
TGCACGTGTT CGTTCCCAAC CAGTGAGCTG GAGAAGGTGC TCCCCCAGAC CATCCTGTAT 
AAGTACTATG AGCGAAAAGC CGAGGAGGAG GTTGCGGCAG CCTACGCCGA CGAGCTTGTC 
AGGTGCCCGT CCTGTAGCTT TCCGGCTCTG TTGGACAGTG ATGTGAAGAG GTTCAGCTGT 
CCTAATCCTC ACTGCCGAAA GGAAACCTGT AGGAAGTGTC AGGGACTCTG GAAAGAACAT 
AATGGCCTCA CCTGTGAAGA GCTGGCTGAA AAAGACGACA TCAAGTACCG TACCTCTATT 
GAAGAAAAAA TGACTGCTGC CCGCATTAGA AAATGCCACA AGTGTGGGAC TGGCCTCATC 
AAATCTGAAG GCTGCAACCG CATGTCTTGC CGCTGTGGTG CCCAGATGTG CTACCTCTGT 
CGAGTTTCTA TTAATGGATA TGACCATTTC TGCCAACATC CCCGCTCACC AGGAGCCCCT 
TGCCAGGAGT GTTCAAGATG CTCTCTCTGG ACCGATCCCA CTGAAGATGA TGAGAAGCTT 
ATTGAGGAAA TCCAGAAGGA GGCTGAAGAG GAACAGAAAA GAAAGAATGG AGAGAACACC 
TTCAAACGCA TTGGACCCCC GCTGGAGAAG CCTGTGGAGA AGGTGCAGAG GGTGGAGGCC 
CTCCCGAGGC CCGTTCCGCA GAACCTGCCA CAGCCACAGA TGCCACCCTA TGCCTTCGCG 
CACCCACCCT TCCCCCTGCC TCCCGTGCGG CCTGTGTTCA ACAACTTCCC ACTCAACATG 
GGGCCTATCC CAGCCCCGTA CGTGCCCCCT CTGCCCAACG TGCGGGTCAA CTATGACTTC 
GGTCCCATCC ACATGCCCCT GGAGCACAAC CTGCCCATGC ACTTTGGCCC CCAGCCGCGG 
CATCGCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_005249785.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249842.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu22477
Clone ID Related Accession (Same CDS sequence)	NM_207111.3 , XM_005249785.2 , NM_207111.4 , XM_024446805.1
Accession Version	XM_005249785.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005249785.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AACAAATAAA CCTCAGAGAT CACGACCCAA TCTCATCAAA 
CCAGCTGCCC AGTGGCAAGA TCTGAAAAGG TTGGGAGAAG AAAGGCCTAA AAAGTCTAGA 
GCAGCATTTG AATCAGATAA GAGCAGCTAT TTTTCAGTGT GTAACAACCC ATTGTTTGAT 
TCTGGGGCAC AGGATGATTC TGAGGATGAC TACGGTGAAT TTCTGGATCT TGGGCCTCCT 
GGAATCTCTG AATTCACTAA GCCAAGTGGC CAAACAGAAA GAGAACCCAA GCCTGGACCG 
AGTCATAACC AAGCAGCAAA TGACATTGTC AACCCCAGAT CAGAGCAGAA AGTCATCATC 
TTGGAAGAAG GTAGCCTTCT TTACACAGAA AGCGATCCTT TGGAAACTCA GAACCAGTCA 
TCCGAAGACT CAGAGACAGA GCTGTTATCA AATCTAGGAG AGTCAGCTGC TCTAGCAGAT 
GATCAGGCCA TCGAAGAAGA CTGCTGGTTA GATCATCCTT ACTTCCAGTC TCTGAACCAA 
CAGCCCCGTG AAATAACAAA CCAGGTCGTT CCTCAGGAAC GGCAGCCTGA AGCAGAACTG 
GGCCGCTTGT TGTTTCAGCA TGAATTCCCA GGGCCCGCTT TTCCAAGGCC GGAACCCCAG 
CAAGGTGGGA TTTCAGGCCC CTCTTCTCCT CAGCCTGCCC ATCCTCTAGG AGAGTTTGAA 
GACCAGCAGT TAGCAAGTGA TGATGAAGAG CCAGGTCCAG CCTTTCCAAT GCAAGAATCT 
CAAGAGCCCA ATTTGGAAAA CATTTGGGGG CAAGAAGCTG CAGAGGTAGA TCAAGAGCTC 
GTTGAACTAC TAGTGAAAGA AACGGAAGCA AGATTTCCAG ATGTAGCAAA TGGGTTTATT 
GAGGAAATAA TTCATTTTAA GAATTATTAT GATCTGAATG TACTTTGTAA TTTTCTTCTG 
GAAAACCCAG ATTATCCAAA GAGAGAAGAC AGAATCATTA TAAATCCCAG TAGCAGTCTG 
CTGGCCAGCC AAGATGAGAC AAAGTTGCCT AAAATAGACT TTTTTGACTA TTCTAAATTG 
ACCCCTCTTG ACCAGCGCTG CTTCATCCAA GCTGCTGACC TCCTCATGGC CGACTTCAAA 
GTGCTCAGTA GTCAGGACAT CAAGTGGGCC CTGCACGAGC TCAAAGGACA CTATGCAATC 
ACCCGAAAGG CCTTGTCTGA TGCCATTAAA AAATGGCAGG AGCTGTCACC AGAAACCAGT 
GGAAAAAGGA AGAAGAGAAA ACAAATGAAC CAGTATTCTT ACATTGATTT CAAGTTTGAA 
CAAGGTGACA TAAAAATAGA AAAGAGGATG TTCTTTCTTG AAAATAAGCG ACGACATTGT 
AGGTCCTATG ACCGACGTGC TCTCCTTCCA GCTGTGCAAC AAGAGCAGGA GTTCTATGAG 
CAGAAAATCA AAGAGATGGC AGAGCATGAA GACTTTTTGC TTGCCCTACA GATGAATGAA 
GAACAGTATC AAAAGGATGG CCAGCTGATT GAGTGTCGCT GCTGCTATGG GGAATTTCCA 
TTCGAGGAGC TGACGCAGTG CGCAGATGCT CACTTGTTCT GCAAAGAGTG TCTCATCAGA 
TATGCCCAAG AGGCAGTCTT TGGATCTGGA AAGTTGGAGC TCAGCTGCAT GGAAGGCAGC 
TGCACGTGTT CGTTCCCAAC CAGTGAGCTG GAGAAGGTGC TCCCCCAGAC CATCCTGTAT 
AAGTACTATG AGCGAAAAGC CGAGGAGGAG GTTGCGGCAG CCTACGCCGA CGAGCTTGTC 
AGGTGCCCGT CCTGTAGCTT TCCGGCTCTG TTGGACAGTG ATGTGAAGAG GTTCAGCTGT 
CCTAATCCTC ACTGCCGAAA GGAAACCTGT AGGAAGTGTC AGGGACTCTG GAAAGAACAT 
AATGGCCTCA CCTGTGAAGA GCTGGCTGAA AAAGACGACA TCAAGTACCG TACCTCTATT 
GAAGAAAAAA TGACTGCTGC CCGCATTAGA AAATGCCACA AGTGTGGGAC TGGCCTCATC 
AAATCTGAAG GCTGCAACCG CATGTCTTGC CGCTGTGGTG CCCAGATGTG CTACCTCTGT 
CGAGTTTCTA TTAATGGATA TGACCATTTC TGCCAACATC CCCGCTCACC AGGAGCCCCT 
TGCCAGGAGT GTTCAAGATG CTCTCTCTGG ACCGATCCCA CTGAAGATGA TGAGAAGCTT 
ATTGAGGAAA TCCAGAAGGA GGCTGAAGAG GAACAGAAAA GAAAGAATGG AGAGAACACC 
TTCAAACGCA TTGGACCCCC GCTGGAGAAG CCTGTGGAGA AGGTGCAGAG GGTGGAGGCC 
CTCCCGAGGC CCGTTCCGCA GAACCTGCCA CAGCCACAGA TGCCACCCTA TGCCTTCGCG 
CACCCACCCT TCCCCCTGCC TCCCGTGCGG CCTGTGTTCA ACAACTTCCC ACTCAACATG 
GGGCCTATCC CAGCCCCGTA CGTGCCCCCT CTGCCCAACG TGCGGGTCAA CTATGACTTC 
GGTCCCATCC ACATGCCCCT GGAGCACAAC CTGCCCATGC ACTTTGGCCC CCAGCCGCGG 
CATCGCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249842.1
CDS	580..3351
Translation	MEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIPMLVTPAPQQHEEEDLDDDVILTETNKPQRSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDKSSYFSVCNNPLFDSGAQDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGPSHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAALADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPRPEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAAEVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRIIINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_005249785.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249785.2

ORF Insert Sequence:

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AACAAATAAA CCTCAGAGAT CACGACCCAA TCTCATCAAA 
CCAGCTGCCC AGTGGCAAGA TCTGAAAAGG TTGGGAGAAG AAAGGCCTAA AAAGTCTAGA 
GCAGCATTTG AATCAGATAA GAGCAGCTAT TTTTCAGTGT GTAACAACCC ATTGTTTGAT 
TCTGGGGCAC AGGATGATTC TGAGGATGAC TACGGTGAAT TTCTGGATCT TGGGCCTCCT 
GGAATCTCTG AATTCACTAA GCCAAGTGGC CAAACAGAAA GAGAACCCAA GCCTGGACCG 
AGTCATAACC AAGCAGCAAA TGACATTGTC AACCCCAGAT CAGAGCAGAA AGTCATCATC 
TTGGAAGAAG GTAGCCTTCT TTACACAGAA AGCGATCCTT TGGAAACTCA GAACCAGTCA 
TCCGAAGACT CAGAGACAGA GCTGTTATCA AATCTAGGAG AGTCAGCTGC TCTAGCAGAT 
GATCAGGCCA TCGAAGAAGA CTGCTGGTTA GATCATCCTT ACTTCCAGTC TCTGAACCAA 
CAGCCCCGTG AAATAACAAA CCAGGTCGTT CCTCAGGAAC GGCAGCCTGA AGCAGAACTG 
GGCCGCTTGT TGTTTCAGCA TGAATTCCCA GGGCCCGCTT TTCCAAGGCC GGAACCCCAG 
CAAGGTGGGA TTTCAGGCCC CTCTTCTCCT CAGCCTGCCC ATCCTCTAGG AGAGTTTGAA 
GACCAGCAGT TAGCAAGTGA TGATGAAGAG CCAGGTCCAG CCTTTCCAAT GCAAGAATCT 
CAAGAGCCCA ATTTGGAAAA CATTTGGGGG CAAGAAGCTG CAGAGGTAGA TCAAGAGCTC 
GTTGAACTAC TAGTGAAAGA AACGGAAGCA AGATTTCCAG ATGTAGCAAA TGGGTTTATT 
GAGGAAATAA TTCATTTTAA GAATTATTAT GATCTGAATG TACTTTGTAA TTTTCTTCTG 
GAAAACCCAG ATTATCCAAA GAGAGAAGAC AGAATCATTA TAAATCCCAG TAGCAGTCTG 
CTGGCCAGCC AAGATGAGAC AAAGTTGCCT AAAATAGACT TTTTTGACTA TTCTAAATTG 
ACCCCTCTTG ACCAGCGCTG CTTCATCCAA GCTGCTGACC TCCTCATGGC CGACTTCAAA 
GTGCTCAGTA GTCAGGACAT CAAGTGGGCC CTGCACGAGC TCAAAGGACA CTATGCAATC 
ACCCGAAAGG CCTTGTCTGA TGCCATTAAA AAATGGCAGG AGCTGTCACC AGAAACCAGT 
GGAAAAAGGA AGAAGAGAAA ACAAATGAAC CAGTATTCTT ACATTGATTT CAAGTTTGAA 
CAAGGTGACA TAAAAATAGA AAAGAGGATG TTCTTTCTTG AAAATAAGCG ACGACATTGT 
AGGTCCTATG ACCGACGTGC TCTCCTTCCA GCTGTGCAAC AAGAGCAGGA GTTCTATGAG 
CAGAAAATCA AAGAGATGGC AGAGCATGAA GACTTTTTGC TTGCCCTACA GATGAATGAA 
GAACAGTATC AAAAGGATGG CCAGCTGATT GAGTGTCGCT GCTGCTATGG GGAATTTCCA 
TTCGAGGAGC TGACGCAGTG CGCAGATGCT CACTTGTTCT GCAAAGAGTG TCTCATCAGA 
TATGCCCAAG AGGCAGTCTT TGGATCTGGA AAGTTGGAGC TCAGCTGCAT GGAAGGCAGC 
TGCACGTGTT CGTTCCCAAC CAGTGAGCTG GAGAAGGTGC TCCCCCAGAC CATCCTGTAT 
AAGTACTATG AGCGAAAAGC CGAGGAGGAG GTTGCGGCAG CCTACGCCGA CGAGCTTGTC 
AGGTGCCCGT CCTGTAGCTT TCCGGCTCTG TTGGACAGTG ATGTGAAGAG GTTCAGCTGT 
CCTAATCCTC ACTGCCGAAA GGAAACCTGT AGGAAGTGTC AGGGACTCTG GAAAGAACAT 
AATGGCCTCA CCTGTGAAGA GCTGGCTGAA AAAGACGACA TCAAGTACCG TACCTCTATT 
GAAGAAAAAA TGACTGCTGC CCGCATTAGA AAATGCCACA AGTGTGGGAC TGGCCTCATC 
AAATCTGAAG GCTGCAACCG CATGTCTTGC CGCTGTGGTG CCCAGATGTG CTACCTCTGT 
CGAGTTTCTA TTAATGGATA TGACCATTTC TGCCAACATC CCCGCTCACC AGGAGCCCCT 
TGCCAGGAGT GTTCAAGATG CTCTCTCTGG ACCGATCCCA CTGAAGATGA TGAGAAGCTT 
ATTGAGGAAA TCCAGAAGGA GGCTGAAGAG GAACAGAAAA GAAAGAATGG AGAGAACACC 
TTCAAACGCA TTGGACCCCC GCTGGAGAAG CCTGTGGAGA AGGTGCAGAG GGTGGAGGCC 
CTCCCGAGGC CCGTTCCGCA GAACCTGCCA CAGCCACAGA TGCCACCCTA TGCCTTCGCG 
CACCCACCCT TCCCCCTGCC TCCCGTGCGG CCTGTGTTCA ACAACTTCCC ACTCAACATG 
GGGCCTATCC CAGCCCCGTA CGTGCCCCCT CTGCCCAACG TGCGGGTCAA CTATGACTTC 
GGTCCCATCC ACATGCCCCT GGAGCACAAC CTGCCCATGC ACTTTGGCCC CCAGCCGCGG 
CATCGCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_024446805.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302573.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu22477
Clone ID Related Accession (Same CDS sequence)	NM_207111.3 , XM_005249785.2 , NM_207111.4 , XM_024446805.1
Accession Version	XM_024446805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AACAAATAAA CCTCAGAGAT CACGACCCAA TCTCATCAAA 
CCAGCTGCCC AGTGGCAAGA TCTGAAAAGG TTGGGAGAAG AAAGGCCTAA AAAGTCTAGA 
GCAGCATTTG AATCAGATAA GAGCAGCTAT TTTTCAGTGT GTAACAACCC ATTGTTTGAT 
TCTGGGGCAC AGGATGATTC TGAGGATGAC TACGGTGAAT TTCTGGATCT TGGGCCTCCT 
GGAATCTCTG AATTCACTAA GCCAAGTGGC CAAACAGAAA GAGAACCCAA GCCTGGACCG 
AGTCATAACC AAGCAGCAAA TGACATTGTC AACCCCAGAT CAGAGCAGAA AGTCATCATC 
TTGGAAGAAG GTAGCCTTCT TTACACAGAA AGCGATCCTT TGGAAACTCA GAACCAGTCA 
TCCGAAGACT CAGAGACAGA GCTGTTATCA AATCTAGGAG AGTCAGCTGC TCTAGCAGAT 
GATCAGGCCA TCGAAGAAGA CTGCTGGTTA GATCATCCTT ACTTCCAGTC TCTGAACCAA 
CAGCCCCGTG AAATAACAAA CCAGGTCGTT CCTCAGGAAC GGCAGCCTGA AGCAGAACTG 
GGCCGCTTGT TGTTTCAGCA TGAATTCCCA GGGCCCGCTT TTCCAAGGCC GGAACCCCAG 
CAAGGTGGGA TTTCAGGCCC CTCTTCTCCT CAGCCTGCCC ATCCTCTAGG AGAGTTTGAA 
GACCAGCAGT TAGCAAGTGA TGATGAAGAG CCAGGTCCAG CCTTTCCAAT GCAAGAATCT 
CAAGAGCCCA ATTTGGAAAA CATTTGGGGG CAAGAAGCTG CAGAGGTAGA TCAAGAGCTC 
GTTGAACTAC TAGTGAAAGA AACGGAAGCA AGATTTCCAG ATGTAGCAAA TGGGTTTATT 
GAGGAAATAA TTCATTTTAA GAATTATTAT GATCTGAATG TACTTTGTAA TTTTCTTCTG 
GAAAACCCAG ATTATCCAAA GAGAGAAGAC AGAATCATTA TAAATCCCAG TAGCAGTCTG 
CTGGCCAGCC AAGATGAGAC AAAGTTGCCT AAAATAGACT TTTTTGACTA TTCTAAATTG 
ACCCCTCTTG ACCAGCGCTG CTTCATCCAA GCTGCTGACC TCCTCATGGC CGACTTCAAA 
GTGCTCAGTA GTCAGGACAT CAAGTGGGCC CTGCACGAGC TCAAAGGACA CTATGCAATC 
ACCCGAAAGG CCTTGTCTGA TGCCATTAAA AAATGGCAGG AGCTGTCACC AGAAACCAGT 
GGAAAAAGGA AGAAGAGAAA ACAAATGAAC CAGTATTCTT ACATTGATTT CAAGTTTGAA 
CAAGGTGACA TAAAAATAGA AAAGAGGATG TTCTTTCTTG AAAATAAGCG ACGACATTGT 
AGGTCCTATG ACCGACGTGC TCTCCTTCCA GCTGTGCAAC AAGAGCAGGA GTTCTATGAG 
CAGAAAATCA AAGAGATGGC AGAGCATGAA GACTTTTTGC TTGCCCTACA GATGAATGAA 
GAACAGTATC AAAAGGATGG CCAGCTGATT GAGTGTCGCT GCTGCTATGG GGAATTTCCA 
TTCGAGGAGC TGACGCAGTG CGCAGATGCT CACTTGTTCT GCAAAGAGTG TCTCATCAGA 
TATGCCCAAG AGGCAGTCTT TGGATCTGGA AAGTTGGAGC TCAGCTGCAT GGAAGGCAGC 
TGCACGTGTT CGTTCCCAAC CAGTGAGCTG GAGAAGGTGC TCCCCCAGAC CATCCTGTAT 
AAGTACTATG AGCGAAAAGC CGAGGAGGAG GTTGCGGCAG CCTACGCCGA CGAGCTTGTC 
AGGTGCCCGT CCTGTAGCTT TCCGGCTCTG TTGGACAGTG ATGTGAAGAG GTTCAGCTGT 
CCTAATCCTC ACTGCCGAAA GGAAACCTGT AGGAAGTGTC AGGGACTCTG GAAAGAACAT 
AATGGCCTCA CCTGTGAAGA GCTGGCTGAA AAAGACGACA TCAAGTACCG TACCTCTATT 
GAAGAAAAAA TGACTGCTGC CCGCATTAGA AAATGCCACA AGTGTGGGAC TGGCCTCATC 
AAATCTGAAG GCTGCAACCG CATGTCTTGC CGCTGTGGTG CCCAGATGTG CTACCTCTGT 
CGAGTTTCTA TTAATGGATA TGACCATTTC TGCCAACATC CCCGCTCACC AGGAGCCCCT 
TGCCAGGAGT GTTCAAGATG CTCTCTCTGG ACCGATCCCA CTGAAGATGA TGAGAAGCTT 
ATTGAGGAAA TCCAGAAGGA GGCTGAAGAG GAACAGAAAA GAAAGAATGG AGAGAACACC 
TTCAAACGCA TTGGACCCCC GCTGGAGAAG CCTGTGGAGA AGGTGCAGAG GGTGGAGGCC 
CTCCCGAGGC CCGTTCCGCA GAACCTGCCA CAGCCACAGA TGCCACCCTA TGCCTTCGCG 
CACCCACCCT TCCCCCTGCC TCCCGTGCGG CCTGTGTTCA ACAACTTCCC ACTCAACATG 
GGGCCTATCC CAGCCCCGTA CGTGCCCCCT CTGCCCAACG TGCGGGTCAA CTATGACTTC 
GGTCCCATCC ACATGCCCCT GGAGCACAAC CTGCCCATGC ACTTTGGCCC CCAGCCGCGG 
CATCGCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302573.1
CDS	256..3027
Translation	MEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIPMLVTPAPQQHEEEDLDDDVILTETNKPQRSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDKSSYFSVCNNPLFDSGAQDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGPSHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAALADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPRPEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAAEVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRIIINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGPPLEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF

Target ORF information:

RefSeq Version	XM_024446805.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446805.1

ORF Insert Sequence:

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AACAAATAAA CCTCAGAGAT CACGACCCAA TCTCATCAAA 
CCAGCTGCCC AGTGGCAAGA TCTGAAAAGG TTGGGAGAAG AAAGGCCTAA AAAGTCTAGA 
GCAGCATTTG AATCAGATAA GAGCAGCTAT TTTTCAGTGT GTAACAACCC ATTGTTTGAT 
TCTGGGGCAC AGGATGATTC TGAGGATGAC TACGGTGAAT TTCTGGATCT TGGGCCTCCT 
GGAATCTCTG AATTCACTAA GCCAAGTGGC CAAACAGAAA GAGAACCCAA GCCTGGACCG 
AGTCATAACC AAGCAGCAAA TGACATTGTC AACCCCAGAT CAGAGCAGAA AGTCATCATC 
TTGGAAGAAG GTAGCCTTCT TTACACAGAA AGCGATCCTT TGGAAACTCA GAACCAGTCA 
TCCGAAGACT CAGAGACAGA GCTGTTATCA AATCTAGGAG AGTCAGCTGC TCTAGCAGAT 
GATCAGGCCA TCGAAGAAGA CTGCTGGTTA GATCATCCTT ACTTCCAGTC TCTGAACCAA 
CAGCCCCGTG AAATAACAAA CCAGGTCGTT CCTCAGGAAC GGCAGCCTGA AGCAGAACTG 
GGCCGCTTGT TGTTTCAGCA TGAATTCCCA GGGCCCGCTT TTCCAAGGCC GGAACCCCAG 
CAAGGTGGGA TTTCAGGCCC CTCTTCTCCT CAGCCTGCCC ATCCTCTAGG AGAGTTTGAA 
GACCAGCAGT TAGCAAGTGA TGATGAAGAG CCAGGTCCAG CCTTTCCAAT GCAAGAATCT 
CAAGAGCCCA ATTTGGAAAA CATTTGGGGG CAAGAAGCTG CAGAGGTAGA TCAAGAGCTC 
GTTGAACTAC TAGTGAAAGA AACGGAAGCA AGATTTCCAG ATGTAGCAAA TGGGTTTATT 
GAGGAAATAA TTCATTTTAA GAATTATTAT GATCTGAATG TACTTTGTAA TTTTCTTCTG 
GAAAACCCAG ATTATCCAAA GAGAGAAGAC AGAATCATTA TAAATCCCAG TAGCAGTCTG 
CTGGCCAGCC AAGATGAGAC AAAGTTGCCT AAAATAGACT TTTTTGACTA TTCTAAATTG 
ACCCCTCTTG ACCAGCGCTG CTTCATCCAA GCTGCTGACC TCCTCATGGC CGACTTCAAA 
GTGCTCAGTA GTCAGGACAT CAAGTGGGCC CTGCACGAGC TCAAAGGACA CTATGCAATC 
ACCCGAAAGG CCTTGTCTGA TGCCATTAAA AAATGGCAGG AGCTGTCACC AGAAACCAGT 
GGAAAAAGGA AGAAGAGAAA ACAAATGAAC CAGTATTCTT ACATTGATTT CAAGTTTGAA 
CAAGGTGACA TAAAAATAGA AAAGAGGATG TTCTTTCTTG AAAATAAGCG ACGACATTGT 
AGGTCCTATG ACCGACGTGC TCTCCTTCCA GCTGTGCAAC AAGAGCAGGA GTTCTATGAG 
CAGAAAATCA AAGAGATGGC AGAGCATGAA GACTTTTTGC TTGCCCTACA GATGAATGAA 
GAACAGTATC AAAAGGATGG CCAGCTGATT GAGTGTCGCT GCTGCTATGG GGAATTTCCA 
TTCGAGGAGC TGACGCAGTG CGCAGATGCT CACTTGTTCT GCAAAGAGTG TCTCATCAGA 
TATGCCCAAG AGGCAGTCTT TGGATCTGGA AAGTTGGAGC TCAGCTGCAT GGAAGGCAGC 
TGCACGTGTT CGTTCCCAAC CAGTGAGCTG GAGAAGGTGC TCCCCCAGAC CATCCTGTAT 
AAGTACTATG AGCGAAAAGC CGAGGAGGAG GTTGCGGCAG CCTACGCCGA CGAGCTTGTC 
AGGTGCCCGT CCTGTAGCTT TCCGGCTCTG TTGGACAGTG ATGTGAAGAG GTTCAGCTGT 
CCTAATCCTC ACTGCCGAAA GGAAACCTGT AGGAAGTGTC AGGGACTCTG GAAAGAACAT 
AATGGCCTCA CCTGTGAAGA GCTGGCTGAA AAAGACGACA TCAAGTACCG TACCTCTATT 
GAAGAAAAAA TGACTGCTGC CCGCATTAGA AAATGCCACA AGTGTGGGAC TGGCCTCATC 
AAATCTGAAG GCTGCAACCG CATGTCTTGC CGCTGTGGTG CCCAGATGTG CTACCTCTGT 
CGAGTTTCTA TTAATGGATA TGACCATTTC TGCCAACATC CCCGCTCACC AGGAGCCCCT 
TGCCAGGAGT GTTCAAGATG CTCTCTCTGG ACCGATCCCA CTGAAGATGA TGAGAAGCTT 
ATTGAGGAAA TCCAGAAGGA GGCTGAAGAG GAACAGAAAA GAAAGAATGG AGAGAACACC 
TTCAAACGCA TTGGACCCCC GCTGGAGAAG CCTGTGGAGA AGGTGCAGAG GGTGGAGGCC 
CTCCCGAGGC CCGTTCCGCA GAACCTGCCA CAGCCACAGA TGCCACCCTA TGCCTTCGCG 
CACCCACCCT TCCCCCTGCC TCCCGTGCGG CCTGTGTTCA ACAACTTCCC ACTCAACATG 
GGGCCTATCC CAGCCCCGTA CGTGCCCCCT CTGCCCAACG TGCGGGTCAA CTATGACTTC 
GGTCCCATCC ACATGCCCCT GGAGCACAAC CTGCCCATGC ACTTTGGCCC CCAGCCGCGG 
CATCGCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_017012364.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867853.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu97361
Clone ID Related Accession (Same CDS sequence)	XM_017012364.1 , XM_017012364.2
Accession Version	XM_017012364.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AACAAATAAA CCTCAGAGAT CACGACCCAA TCTCATCAAA 
CCAGCTGCCC AGTGGCAAGA TCTGAAAAGG TTGGGAGAAG AAAGGCCTAA AAAGTCTAGA 
GCAGCATTTG AATCAGATAA GAGCAGCTAT TTTTCAGTGT GTAACAACCC ATTGTTTGAT 
TCTGGGGCAC AGGATGATTC TGAGGATGAC TACGGTGAAT TTCTGGATCT TGGGCCTCCT 
GGAATCTCTG AATTCACTAA GCCAAGTGGC CAAACAGAAA GAGAACCCAA GCCTGGACCG 
AGTCATAACC AAGCAGCAAA TGACATTGTC AACCCCAGAT CAGAGCAGAA AGTCATCATC 
TTGGAAGAAG GTAGCCTTCT TTACACAGAA AGCGATCCTT TGGAAACTCA GAACCAGTCA 
TCCGAAGACT CAGAGACAGA GCTGTTATCA AATCTAGGAG AGTCAGCTGC TCTAGCAGAT 
GATCAGGCCA TCGAAGAAGA CTGCTGGTTA GATCATCCTT ACTTCCAGTC TCTGAACCAA 
CAGCCCCGTG AAATAACAAA CCAGGTCGTT CCTCAGGAAC GGCAGCCTGA AGCAGAACTG 
GGCCGCTTGT TGTTTCAGCA TGAATTCCCA GGGCCCGCTT TTCCAAGGCC GGAACCCCAG 
CAAGGTGGGA TTTCAGGCCC CTCTTCTCCT CAGCCTGCCC ATCCTCTAGG AGAGTTTGAA 
GACCAGCAGT TAGCAAGTGA TGATGAAGAG CCAGGTCCAG CCTTTCCAAT GCAAGAATCT 
CAAGAGCCCA ATTTGGAAAA CATTTGGGGG CAAGAAGCTG CAGAGGTAGA TCAAGAGCTC 
GTTGAACTAC TAGTGAAAGA AACGGAAGCA AGATTTCCAG ATGTAGCAAA TGGGTTTATT 
GAGGAAATAA TTCATTTTAA GAATTATTAT GATCTGAATG TACTTTGTAA TTTTCTTCTG 
GAAAACCCAG ATTATCCAAA GAGAGAAGAC AGAATCATTA TAAATCCCAG TAGCAGTCTG 
CTGGCCAGCC AAGATGAGAC AAAGTTGCCT AAAATAGACT TTTTTGACTA TTCTAAATTG 
ACCCCTCTTG ACCAGCGCTG CTTCATCCAA GCTGCTGACC TCCTCATGGC CGACTTCAAA 
GTGCTCAGTA GTCAGGACAT CAAGTGGGCC CTGCACGAGC TCAAAGGACA CTATGCAATC 
ACCCGAAAGG CCTTGTCTGA TGCCATTAAA AAATGGCAGG AGCTGTCACC AGAAACCAGT 
GGAAAAAGGA AGAAGAGAAA ACAAATGAAC CAGTATTCTT ACATTGATTT CAAGTTTGAA 
CAAGGTGACA TAAAAATAGA AAAGAGGATG TTCTTTCTTG AAAATAAGCG ACGACATTGT 
AGGTCCTATG ACCGACGTGC TCTCCTTCCA GCTGTGCAAC AAGAGCAGGA GTTCTATGAG 
CAGAAAATCA AAGAGATGGC AGAGCATGAA GACTTTTTGC TTGCCCTACA GATGAATGAA 
GAACAGTATC AAAAGGATGG CCAGCTGATT GAGTGTCGCT GCTGCTATGG GGAATTTCCA 
TTCGAGGAGC TGACGCAGTG CGCAGATGCT CACTTGTTCT GCAAAGAGTG TCTCATCAGA 
TATGCCCAAG AGGCAGTCTT TGGATCTGGA AAGTTGGAGC TCAGCTGCAT GGAAGGCAGC 
TGCACGTGTT CGTTCCCAAC CAGTGAGCTG GAGAAGGTGC TCCCCCAGAC CATCCTGTAT 
AAGTACTATG AGCGAAAAGC CGAGGAGGAG GTTGCGGCAG CCTACGCCGA CGAGCTTGTC 
AGGTGCCCGT CCTGTAGCTT TCCGGCTCTG TTGGACAGTG ATGTGAAGAG GTTCAGCTGT 
CCTAATCCTC ACTGCCGAAA GGAAACCTGT AGGAAGTGTC AGGGACTCTG GAAAGAACAT 
AATGGCCTCA CCTGTGAAGA GCTGGCTGAA AAAGACGACA TCAAGTACCG TACCTCTATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867853.1
CDS	233..2395
Translation	MEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIPMLVTPAPQQHEEEDLDDDVILTETNKPQRSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDKSSYFSVCNNPLFDSGAQDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGPSHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAALADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPRPEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAAEVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRIIINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSM

Target ORF information:

RefSeq Version	XM_017012364.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012364.1

ORF Insert Sequence:

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AACAAATAAA CCTCAGAGAT CACGACCCAA TCTCATCAAA 
CCAGCTGCCC AGTGGCAAGA TCTGAAAAGG TTGGGAGAAG AAAGGCCTAA AAAGTCTAGA 
GCAGCATTTG AATCAGATAA GAGCAGCTAT TTTTCAGTGT GTAACAACCC ATTGTTTGAT 
TCTGGGGCAC AGGATGATTC TGAGGATGAC TACGGTGAAT TTCTGGATCT TGGGCCTCCT 
GGAATCTCTG AATTCACTAA GCCAAGTGGC CAAACAGAAA GAGAACCCAA GCCTGGACCG 
AGTCATAACC AAGCAGCAAA TGACATTGTC AACCCCAGAT CAGAGCAGAA AGTCATCATC 
TTGGAAGAAG GTAGCCTTCT TTACACAGAA AGCGATCCTT TGGAAACTCA GAACCAGTCA 
TCCGAAGACT CAGAGACAGA GCTGTTATCA AATCTAGGAG AGTCAGCTGC TCTAGCAGAT 
GATCAGGCCA TCGAAGAAGA CTGCTGGTTA GATCATCCTT ACTTCCAGTC TCTGAACCAA 
CAGCCCCGTG AAATAACAAA CCAGGTCGTT CCTCAGGAAC GGCAGCCTGA AGCAGAACTG 
GGCCGCTTGT TGTTTCAGCA TGAATTCCCA GGGCCCGCTT TTCCAAGGCC GGAACCCCAG 
CAAGGTGGGA TTTCAGGCCC CTCTTCTCCT CAGCCTGCCC ATCCTCTAGG AGAGTTTGAA 
GACCAGCAGT TAGCAAGTGA TGATGAAGAG CCAGGTCCAG CCTTTCCAAT GCAAGAATCT 
CAAGAGCCCA ATTTGGAAAA CATTTGGGGG CAAGAAGCTG CAGAGGTAGA TCAAGAGCTC 
GTTGAACTAC TAGTGAAAGA AACGGAAGCA AGATTTCCAG ATGTAGCAAA TGGGTTTATT 
GAGGAAATAA TTCATTTTAA GAATTATTAT GATCTGAATG TACTTTGTAA TTTTCTTCTG 
GAAAACCCAG ATTATCCAAA GAGAGAAGAC AGAATCATTA TAAATCCCAG TAGCAGTCTG 
CTGGCCAGCC AAGATGAGAC AAAGTTGCCT AAAATAGACT TTTTTGACTA TTCTAAATTG 
ACCCCTCTTG ACCAGCGCTG CTTCATCCAA GCTGCTGACC TCCTCATGGC CGACTTCAAA 
GTGCTCAGTA GTCAGGACAT CAAGTGGGCC CTGCACGAGC TCAAAGGACA CTATGCAATC 
ACCCGAAAGG CCTTGTCTGA TGCCATTAAA AAATGGCAGG AGCTGTCACC AGAAACCAGT 
GGAAAAAGGA AGAAGAGAAA ACAAATGAAC CAGTATTCTT ACATTGATTT CAAGTTTGAA 
CAAGGTGACA TAAAAATAGA AAAGAGGATG TTCTTTCTTG AAAATAAGCG ACGACATTGT 
AGGTCCTATG ACCGACGTGC TCTCCTTCCA GCTGTGCAAC AAGAGCAGGA GTTCTATGAG 
CAGAAAATCA AAGAGATGGC AGAGCATGAA GACTTTTTGC TTGCCCTACA GATGAATGAA 
GAACAGTATC AAAAGGATGG CCAGCTGATT GAGTGTCGCT GCTGCTATGG GGAATTTCCA 
TTCGAGGAGC TGACGCAGTG CGCAGATGCT CACTTGTTCT GCAAAGAGTG TCTCATCAGA 
TATGCCCAAG AGGCAGTCTT TGGATCTGGA AAGTTGGAGC TCAGCTGCAT GGAAGGCAGC 
TGCACGTGTT CGTTCCCAAC CAGTGAGCTG GAGAAGGTGC TCCCCCAGAC CATCCTGTAT 
AAGTACTATG AGCGAAAAGC CGAGGAGGAG GTTGCGGCAG CCTACGCCGA CGAGCTTGTC 
AGGTGCCCGT CCTGTAGCTT TCCGGCTCTG TTGGACAGTG ATGTGAAGAG GTTCAGCTGT 
CCTAATCCTC ACTGCCGAAA GGAAACCTGT AGGAAGTGTC AGGGACTCTG GAAAGAACAT 
AATGGCCTCA CCTGTGAAGA GCTGGCTGAA AAAGACGACA TCAAGTACCG TACCTCTATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF216 ( XM_017012364.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867853.1 Homo sapiens ring finger protein 216 (RNF216), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu97361
Clone ID Related Accession (Same CDS sequence)	XM_017012364.1 , XM_017012364.2
Accession Version	XM_017012364.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF216 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017012364.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AACAAATAAA CCTCAGAGAT CACGACCCAA TCTCATCAAA 
CCAGCTGCCC AGTGGCAAGA TCTGAAAAGG TTGGGAGAAG AAAGGCCTAA AAAGTCTAGA 
GCAGCATTTG AATCAGATAA GAGCAGCTAT TTTTCAGTGT GTAACAACCC ATTGTTTGAT 
TCTGGGGCAC AGGATGATTC TGAGGATGAC TACGGTGAAT TTCTGGATCT TGGGCCTCCT 
GGAATCTCTG AATTCACTAA GCCAAGTGGC CAAACAGAAA GAGAACCCAA GCCTGGACCG 
AGTCATAACC AAGCAGCAAA TGACATTGTC AACCCCAGAT CAGAGCAGAA AGTCATCATC 
TTGGAAGAAG GTAGCCTTCT TTACACAGAA AGCGATCCTT TGGAAACTCA GAACCAGTCA 
TCCGAAGACT CAGAGACAGA GCTGTTATCA AATCTAGGAG AGTCAGCTGC TCTAGCAGAT 
GATCAGGCCA TCGAAGAAGA CTGCTGGTTA GATCATCCTT ACTTCCAGTC TCTGAACCAA 
CAGCCCCGTG AAATAACAAA CCAGGTCGTT CCTCAGGAAC GGCAGCCTGA AGCAGAACTG 
GGCCGCTTGT TGTTTCAGCA TGAATTCCCA GGGCCCGCTT TTCCAAGGCC GGAACCCCAG 
CAAGGTGGGA TTTCAGGCCC CTCTTCTCCT CAGCCTGCCC ATCCTCTAGG AGAGTTTGAA 
GACCAGCAGT TAGCAAGTGA TGATGAAGAG CCAGGTCCAG CCTTTCCAAT GCAAGAATCT 
CAAGAGCCCA ATTTGGAAAA CATTTGGGGG CAAGAAGCTG CAGAGGTAGA TCAAGAGCTC 
GTTGAACTAC TAGTGAAAGA AACGGAAGCA AGATTTCCAG ATGTAGCAAA TGGGTTTATT 
GAGGAAATAA TTCATTTTAA GAATTATTAT GATCTGAATG TACTTTGTAA TTTTCTTCTG 
GAAAACCCAG ATTATCCAAA GAGAGAAGAC AGAATCATTA TAAATCCCAG TAGCAGTCTG 
CTGGCCAGCC AAGATGAGAC AAAGTTGCCT AAAATAGACT TTTTTGACTA TTCTAAATTG 
ACCCCTCTTG ACCAGCGCTG CTTCATCCAA GCTGCTGACC TCCTCATGGC CGACTTCAAA 
GTGCTCAGTA GTCAGGACAT CAAGTGGGCC CTGCACGAGC TCAAAGGACA CTATGCAATC 
ACCCGAAAGG CCTTGTCTGA TGCCATTAAA AAATGGCAGG AGCTGTCACC AGAAACCAGT 
GGAAAAAGGA AGAAGAGAAA ACAAATGAAC CAGTATTCTT ACATTGATTT CAAGTTTGAA 
CAAGGTGACA TAAAAATAGA AAAGAGGATG TTCTTTCTTG AAAATAAGCG ACGACATTGT 
AGGTCCTATG ACCGACGTGC TCTCCTTCCA GCTGTGCAAC AAGAGCAGGA GTTCTATGAG 
CAGAAAATCA AAGAGATGGC AGAGCATGAA GACTTTTTGC TTGCCCTACA GATGAATGAA 
GAACAGTATC AAAAGGATGG CCAGCTGATT GAGTGTCGCT GCTGCTATGG GGAATTTCCA 
TTCGAGGAGC TGACGCAGTG CGCAGATGCT CACTTGTTCT GCAAAGAGTG TCTCATCAGA 
TATGCCCAAG AGGCAGTCTT TGGATCTGGA AAGTTGGAGC TCAGCTGCAT GGAAGGCAGC 
TGCACGTGTT CGTTCCCAAC CAGTGAGCTG GAGAAGGTGC TCCCCCAGAC CATCCTGTAT 
AAGTACTATG AGCGAAAAGC CGAGGAGGAG GTTGCGGCAG CCTACGCCGA CGAGCTTGTC 
AGGTGCCCGT CCTGTAGCTT TCCGGCTCTG TTGGACAGTG ATGTGAAGAG GTTCAGCTGT 
CCTAATCCTC ACTGCCGAAA GGAAACCTGT AGGAAGTGTC AGGGACTCTG GAAAGAACAT 
AATGGCCTCA CCTGTGAAGA GCTGGCTGAA AAAGACGACA TCAAGTACCG TACCTCTATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867853.1
CDS	251..2413
Translation	MEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIPMLVTPAPQQHEEEDLDDDVILTETNKPQRSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDKSSYFSVCNNPLFDSGAQDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGPSHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAALADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPRPEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAAEVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRIIINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSM

Target ORF information:

RefSeq Version	XM_017012364.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 216 (RNF216), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012364.2

ORF Insert Sequence:

ATGGAAGAGG GAAACAACAA TGAAGAGGTA ATTCACTTGA ACAACTTTCA CTGCCATCGG 
GGACAAGAGT GGATCAATCT CCGAGATGGG CCCATCACCA TATCTGACTC CTCAGATGAG 
GAAAGGATTC CAATGCTGGT CACCCCAGCT CCTCAGCAGC ATGAAGAAGA GGACCTGGAT 
GATGATGTCA TCCTGACAGA AACAAATAAA CCTCAGAGAT CACGACCCAA TCTCATCAAA 
CCAGCTGCCC AGTGGCAAGA TCTGAAAAGG TTGGGAGAAG AAAGGCCTAA AAAGTCTAGA 
GCAGCATTTG AATCAGATAA GAGCAGCTAT TTTTCAGTGT GTAACAACCC ATTGTTTGAT 
TCTGGGGCAC AGGATGATTC TGAGGATGAC TACGGTGAAT TTCTGGATCT TGGGCCTCCT 
GGAATCTCTG AATTCACTAA GCCAAGTGGC CAAACAGAAA GAGAACCCAA GCCTGGACCG 
AGTCATAACC AAGCAGCAAA TGACATTGTC AACCCCAGAT CAGAGCAGAA AGTCATCATC 
TTGGAAGAAG GTAGCCTTCT TTACACAGAA AGCGATCCTT TGGAAACTCA GAACCAGTCA 
TCCGAAGACT CAGAGACAGA GCTGTTATCA AATCTAGGAG AGTCAGCTGC TCTAGCAGAT 
GATCAGGCCA TCGAAGAAGA CTGCTGGTTA GATCATCCTT ACTTCCAGTC TCTGAACCAA 
CAGCCCCGTG AAATAACAAA CCAGGTCGTT CCTCAGGAAC GGCAGCCTGA AGCAGAACTG 
GGCCGCTTGT TGTTTCAGCA TGAATTCCCA GGGCCCGCTT TTCCAAGGCC GGAACCCCAG 
CAAGGTGGGA TTTCAGGCCC CTCTTCTCCT CAGCCTGCCC ATCCTCTAGG AGAGTTTGAA 
GACCAGCAGT TAGCAAGTGA TGATGAAGAG CCAGGTCCAG CCTTTCCAAT GCAAGAATCT 
CAAGAGCCCA ATTTGGAAAA CATTTGGGGG CAAGAAGCTG CAGAGGTAGA TCAAGAGCTC 
GTTGAACTAC TAGTGAAAGA AACGGAAGCA AGATTTCCAG ATGTAGCAAA TGGGTTTATT 
GAGGAAATAA TTCATTTTAA GAATTATTAT GATCTGAATG TACTTTGTAA TTTTCTTCTG 
GAAAACCCAG ATTATCCAAA GAGAGAAGAC AGAATCATTA TAAATCCCAG TAGCAGTCTG 
CTGGCCAGCC AAGATGAGAC AAAGTTGCCT AAAATAGACT TTTTTGACTA TTCTAAATTG 
ACCCCTCTTG ACCAGCGCTG CTTCATCCAA GCTGCTGACC TCCTCATGGC CGACTTCAAA 
GTGCTCAGTA GTCAGGACAT CAAGTGGGCC CTGCACGAGC TCAAAGGACA CTATGCAATC 
ACCCGAAAGG CCTTGTCTGA TGCCATTAAA AAATGGCAGG AGCTGTCACC AGAAACCAGT 
GGAAAAAGGA AGAAGAGAAA ACAAATGAAC CAGTATTCTT ACATTGATTT CAAGTTTGAA 
CAAGGTGACA TAAAAATAGA AAAGAGGATG TTCTTTCTTG AAAATAAGCG ACGACATTGT 
AGGTCCTATG ACCGACGTGC TCTCCTTCCA GCTGTGCAAC AAGAGCAGGA GTTCTATGAG 
CAGAAAATCA AAGAGATGGC AGAGCATGAA GACTTTTTGC TTGCCCTACA GATGAATGAA 
GAACAGTATC AAAAGGATGG CCAGCTGATT GAGTGTCGCT GCTGCTATGG GGAATTTCCA 
TTCGAGGAGC TGACGCAGTG CGCAGATGCT CACTTGTTCT GCAAAGAGTG TCTCATCAGA 
TATGCCCAAG AGGCAGTCTT TGGATCTGGA AAGTTGGAGC TCAGCTGCAT GGAAGGCAGC 
TGCACGTGTT CGTTCCCAAC CAGTGAGCTG GAGAAGGTGC TCCCCCAGAC CATCCTGTAT 
AAGTACTATG AGCGAAAAGC CGAGGAGGAG GTTGCGGCAG CCTACGCCGA CGAGCTTGTC 
AGGTGCCCGT CCTGTAGCTT TCCGGCTCTG TTGGACAGTG ATGTGAAGAG GTTCAGCTGT 
CCTAATCCTC ACTGCCGAAA GGAAACCTGT AGGAAGTGTC AGGGACTCTG GAAAGAACAT 
AATGGCCTCA CCTGTGAAGA GCTGGCTGAA AAAGACGACA TCAAGTACCG TACCTCTATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RNF216
Entrez Gene ID	54476
Full Name	ring finger protein 216
Synonyms	CAHH,TRIAD3,U7I1,UBCE7IP1,ZIN
General protein information	Preferred Names ring finger protein 216 Names E3 ubiquitin-protein ligase RNF216 RING-type E3 ubiquitin transferase RNF216 triad domain-containing protein 3 ubiquitin-conjugating enzyme 7-interacting protein 1 zinc finger protein inhibiting NF-kappa-B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p22.1
Summary	This gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known. [provided by RefSeq, Jul 2008].
Disorder MIM:	609948
Disorder Html:	Cerebellar ataxia and hypogonadotropic hypogonadism, 212840 (3)

Mus musculus (house mouse)	Rnf216	NP_996993.1
Xenopus tropicalis (tropical clawed frog)	rnf216	NP_001016834.1
Rattus norvegicus (Norway rat)	Rnf216	NP_001100592.1
	RNF216	XP_002803244.1
Danio rerio (zebrafish)	rnf216	XP_687871.3
Homo sapiens (human)	RNF216	NP_996994.1
Bos taurus (cattle)	RNF216	NP_001180187.1
Pan troglodytes (chimpanzee)	RNF216	XP_518955.2
Canis lupus familiaris (dog)	RNF216	XP_536887.2
Gallus gallus (chicken)	RNF216	XP_414793.4

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RNF216 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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