PON3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PON3
Entrez Gene ID	5446
Full Name	paraoxonase 3
General protein information	Preferred Names paraoxonase 3 Names serum paraoxonase/lactonase 3 arylesterase 3 paraoxanase-3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q21.3
Summary	This gene is a member of the paraoxonase family and lies in a cluster on chromosome 7 with the other two family members. The encoded protein is secreted into the bloodstream and associates with high-density lipoprotein (HDL). The protein also rapidly hydrolyzes lactones and can inhibit the oxidation of low-density lipoprotein (LDL), a function that is believed to slow the initiation and progression of atherosclerosis. Alternatively spliced variants which encode different protein isoforms have been described; however, only one has been fully characterized. [provided by RefSeq, Jul 2008].
Disorder MIM:	602720

mRNA and Protein(s)

mRNA	Protein	Name
NM_000940.3	NP_000931.1	serum paraoxonase/lactonase 3
NM_000940.2	NP_000931.1	serum paraoxonase/lactonase 3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pon3	NP_001004086.1
Caenorhabditis elegans (roundworm)	poml-2	NP_491306.1
Homo sapiens (human)	PON3	NP_000931.1
Pan troglodytes (chimpanzee)	PON3	XP_519212.2
Canis lupus familiaris (dog)	PON3	XP_532467.1
Mus musculus (house mouse)	Pon3	NP_766594.1
Caenorhabditis elegans (roundworm)	poml-3	NP_001122440.1
	PON3	XP_001095770.1
Bos taurus (cattle)	PON3	NP_001068947.1
Caenorhabditis elegans (roundworm)	poml-4	NP_001040790.2

Gene Ontology
Bibliography

Related articles in PubMed

Effect of lipoic acid on paraoxonase-1 and paraoxonase-3 protein levels, mRNA expression and arylesterase activity in liver hepatoma cells.
Ozgun E, Sayilan Ozgun G, Tabakcioglu K, Suer Gokmen S, Sut N, Eskiocak S
General physiology and biophysics36(4)465-470(2017 Oct)

Paraoxonases and infectious diseases.
Camps J, Iftimie S, Garc?a-Heredia A, Castro A, Joven J
Clinical biochemistry50(13-14)804-811(2017 Sep)

Paraoxonases and psoriasis: negative imbalance of anti--oxidant endogenous mechanisms.
Schiattarella M, Caiazzo G, DI Caprio R, Lembo S, Raimondo A, Ayala F, Balato N, Monfrecola G, Fortunato G, Balato A
Giornale italiano di dermatologia e venereologia : organo ufficiale, Societa italiana di dermatologia e sifilografia(2017 May)

Ocular distribution of antioxidant enzyme paraoxonase & its alteration in cataractous lens & diabetic retina.
Bharathidevi SR, Babu KA, Jain N, Muthukumaran S, Umashankar V, Biswas J, Angayarkanni N
The Indian journal of medical research145(4)513-520(2017 Apr)

Paraoxonase: The Universal Factor of Antioxidant Defense in Human Body.
Borovkova EI, Antipova NV, Komeenko TV, Shakhparonov MI, Borovkov IM
Vestnik Rossiiskoi akademii meditsinskikh nauk72(1)5-10(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our study showed that although lipoic acid up-regulates PON3 but down-regulates PON1 mRNA expression, it increases both PON1 and PON3 protein levels and arylesterase activity in HepG2 cells. We can report that lipoic acid may be useful for preventing atherosclerosis at therapeutic doses.
Title: Effect of lipoic acid on paraoxonase-1 and paraoxonase-3 protein levels, mRNA expression and arylesterase activity in liver hepatoma cells.

Data suggest that paraoxonases (PON1, PON2, PON3) play roles in innate immunity, inflammatory response, and protection against oxidative stress; these factors are associated with the body's response to infectious diseases; low serum PON1 activities are associated with poor survival in patients with severe sepsis. [REVIEW]
Title: Paraoxonases and infectious diseases.

Studies indicate that three paraoxonases PON1, PON2, and PON3 genes are clustered on chromosome 7, and that PONs possess numerous atheroprotective properties.
Title: Paraoxonase and atherosclerosis-related cardiovascular diseases.

This study showed that the PI3K/Akt pathway is up-regulated by the expression of PON3 in oral squamous cell carcinoma by AP-1.
Title: Paraoxonase 3 promotes cell proliferation and metastasis by PI3K/Akt in oral squamous cell carcinoma.

Data show that the mRNA levels of both paraoxonases PON2 and PON3 were significantly upregulated whereas PON1's mRNA was absent in transgenic mouse hearts.
Title: Human paraoxonase gene cluster overexpression alleviates angiotensin II-induced cardiac hypertrophy in mice.

The following PON3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PON3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17910	NM_000940.3 Latest version!	Homo sapiens paraoxonase 3 (PON3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
View Old Accession Versions >>
OHu17910	NM_000940.2	Homo sapiens paraoxonase 3 (PON3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PON3 ( NM_000940.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000931.1 Homo sapiens paraoxonase 3 (PON3), mRNA.

Price & Availability↑

CloneID	OHu17910
Clone ID Related Accession (Same CDS sequence)	NM_000940.3 , NM_000940.2
Accession Version	NM_000940.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	serum paraoxonase/lactonase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV655538.1, BC020908.1, BC070374.1, CD014095.1 and AA860930.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000940.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189664.112051.1, SRR5189664.86843.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265627.10/ ENSP00000265627.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGAAGC TCGTGGCGCT GGTCCTGCTG GGGGTCGGCC TGTCCTTAGT CGGGGAGATG 
TTCCTGGCGT TTAGAGAAAG GGTGAATGCC TCTCGAGAAG TGGAGCCAGT AGAACCTGAA 
AACTGCCACC TTATTGAGGA ACTTGAAAGT GGCTCTGAAG ATATTGATAT ACTTCCTAGT 
GGGCTGGCTT TTATCTCCAG TGGATTAAAA TATCCAGGCA TGCCAAACTT TGCGCCAGAT 
GAACCAGGAA AAATCTTCTT GATGGATCTG AATGAACAAA ACCCAAGGGC ACAAGCGCTA 
GAAATCAGTG GTGGATTTGA CAAAGAATTA TTTAATCCAC ATGGGATCAG TATTTTCATC 
GACAAAGACA ATACTGTGTA TCTTTATGTT GTGAATCATC CCCACATGAA GTCCACTGTG 
GAGATATTTA AATTTGAGGA ACAACAACGT TCTCTGGTAT ACCTGAAAAC TATAAAACAT 
GAACTTCTCA AAAGTGTGAA TGACATTGTG GTTCTTGGAC CAGAACAGTT CTATGCCACC 
AGAGACCACT ATTTTACCAA CTCCCTCCTG TCATTTTTTG AGATGATCTT GGATCTTCGC 
TGGACTTATG TTCTTTTCTA CAGCCCAAGG GAGGTTAAAG TGGTGGCCAA AGGATTTTGT 
AGTGCCAATG GGATCACAGT CTCAGCAGAC CAGAAGTATG TCTATGTAGC TGATGTAGCA 
GCTAAGAACA TTCACATAAT GGAAAAACAT GATAACTGGG ATTTAACTCA ACTGAAGGTG 
ATACAGTTGG GCACCTTAGT GGATAACCTG ACTGTCGATC CTGCCACAGG AGACATTTTG 
GCAGGATGCC ATCCTAATCC TATGAAGCTA CTGAACTATA ACCCTGAGGA CCCTCCAGGA 
TCAGAAGTAC TTCGCATCCA GAATGTTTTG TCTGAGAAGC CCAGGGTGAG CACCGTGTAT 
GCCAACAATG GCTCTGTGCT TCAGGGCACC TCTGTGGCTT CTGTGTACCA TGGGAAAATT 
CTCATAGGCA CCGTATTTCA CAAAACTCTG TACTGTGAGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000931.1
CDS	26..1090
Translation	MGKLVALVLLGVGLSLVGEMFLAFRERVNASREVEPVEPENCHLIEELESGSEDIDILPSGLAFISSGLKYPGMPNFAPDEPGKIFLMDLNEQNPRAQALEISGGFDKELFNPHGISIFIDKDNTVYLYVVNHPHMKSTVEIFKFEEQQRSLVYLKTIKHELLKSVNDIVVLGPEQFYATRDHYFTNSLLSFFEMILDLRWTYVLFYSPREVKVVAKGFCSANGITVSADQKYVYVADVAAKNIHIMEKHDNWDLTQLKVIQLGTLVDNLTVDPATGDILAGCHPNPMKLLNYNPEDPPGSEVLRIQNVLSEKPRVSTVYANNGSVLQGTSVASVYHGKILIGTVFHKTLYCEL

Target ORF information:

RefSeq Version	NM_000940.3
Organism	Homo sapiens(human)
Definition	Homo sapiens paraoxonase 3 (PON3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000940.3

ORF Insert Sequence:

ATGGGGAAGC TCGTGGCGCT GGTCCTGCTG GGGGTCGGCC TGTCCTTAGT CGGGGAGATG 
TTCCTGGCGT TTAGAGAAAG GGTGAATGCC TCTCGAGAAG TGGAGCCAGT AGAACCTGAA 
AACTGCCACC TTATTGAGGA ACTTGAAAGT GGCTCTGAAG ATATTGATAT ACTTCCTAGT 
GGGCTGGCTT TTATCTCCAG TGGATTAAAA TATCCAGGCA TGCCAAACTT TGCGCCAGAT 
GAACCAGGAA AAATCTTCTT GATGGATCTG AATGAACAAA ACCCAAGGGC ACAAGCGCTA 
GAAATCAGTG GTGGATTTGA CAAAGAATTA TTTAATCCAC ATGGGATCAG TATTTTCATC 
GACAAAGACA ATACTGTGTA TCTTTATGTT GTGAATCATC CCCACATGAA GTCCACTGTG 
GAGATATTTA AATTTGAGGA ACAACAACGT TCTCTGGTAT ACCTGAAAAC TATAAAACAT 
GAACTTCTCA AAAGTGTGAA TGACATTGTG GTTCTTGGAC CAGAACAGTT CTATGCCACC 
AGAGACCACT ATTTTACCAA CTCCCTCCTG TCATTTTTTG AGATGATCTT GGATCTTCGC 
TGGACTTATG TTCTTTTCTA CAGCCCAAGG GAGGTTAAAG TGGTGGCCAA AGGATTTTGT 
AGTGCCAATG GGATCACAGT CTCAGCAGAC CAGAAGTATG TCTATGTAGC TGATGTAGCA 
GCTAAGAACA TTCACATAAT GGAAAAACAT GATAACTGGG ATTTAACTCA ACTGAAGGTG 
ATACAGTTGG GCACCTTAGT GGATAACCTG ACTGTCGATC CTGCCACAGG AGACATTTTG 
GCAGGATGCC ATCCTAATCC TATGAAGCTA CTGAACTATA ACCCTGAGGA CCCTCCAGGA 
TCAGAAGTAC TTCGCATCCA GAATGTTTTG TCTGAGAAGC CCAGGGTGAG CACCGTGTAT 
GCCAACAATG GCTCTGTGCT TCAGGGCACC TCTGTGGCTT CTGTGTACCA TGGGAAAATT 
CTCATAGGCA CCGTATTTCA CAAAACTCTG TACTGTGAGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PON3 ( NM_000940.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000931.1 Homo sapiens paraoxonase 3 (PON3), mRNA.

Price & Availability↑

CloneID	OHu17910
Clone ID Related Accession (Same CDS sequence)	NM_000940.3 , NM_000940.2
Accession Version	NM_000940.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Homo sapiens(human)
Product	serum paraoxonase/lactonase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV655538.1, BC020908.1, BC070374.1, CD014095.1 and AA860930.1. This sequence is a reference standard in the RefSeqGene project. On May 4, 2006 this sequence version replaced NM_000940.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070374.1, AF329433.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAAGC TCGTGGCGCT GGTCCTGCTG GGGGTCGGCC TGTCCTTAGT CGGGGAGATG 
TTCCTGGCGT TTAGAGAAAG GGTGAATGCC TCTCGAGAAG TGGAGCCAGT AGAACCTGAA 
AACTGCCACC TTATTGAGGA ACTTGAAAGT GGCTCTGAAG ATATTGATAT ACTTCCTAGT 
GGGCTGGCTT TTATCTCCAG TGGATTAAAA TATCCAGGCA TGCCAAACTT TGCGCCAGAT 
GAACCAGGAA AAATCTTCTT GATGGATCTG AATGAACAAA ACCCAAGGGC ACAAGCGCTA 
GAAATCAGTG GTGGATTTGA CAAAGAATTA TTTAATCCAC ATGGGATCAG TATTTTCATC 
GACAAAGACA ATACTGTGTA TCTTTATGTT GTGAATCATC CCCACATGAA GTCCACTGTG 
GAGATATTTA AATTTGAGGA ACAACAACGT TCTCTGGTAT ACCTGAAAAC TATAAAACAT 
GAACTTCTCA AAAGTGTGAA TGACATTGTG GTTCTTGGAC CAGAACAGTT CTATGCCACC 
AGAGACCACT ATTTTACCAA CTCCCTCCTG TCATTTTTTG AGATGATCTT GGATCTTCGC 
TGGACTTATG TTCTTTTCTA CAGCCCAAGG GAGGTTAAAG TGGTGGCCAA AGGATTTTGT 
AGTGCCAATG GGATCACAGT CTCAGCAGAC CAGAAGTATG TCTATGTAGC TGATGTAGCA 
GCTAAGAACA TTCACATAAT GGAAAAACAT GATAACTGGG ATTTAACTCA ACTGAAGGTG 
ATACAGTTGG GCACCTTAGT GGATAACCTG ACTGTCGATC CTGCCACAGG AGACATTTTG 
GCAGGATGCC ATCCTAATCC TATGAAGCTA CTGAACTATA ACCCTGAGGA CCCTCCAGGA 
TCAGAAGTAC TTCGCATCCA GAATGTTTTG TCTGAGAAGC CCAGGGTGAG CACCGTGTAT 
GCCAACAATG GCTCTGTGCT TCAGGGCACC TCTGTGGCTT CTGTGTACCA TGGGAAAATT 
CTCATAGGCA CCGTATTTCA CAAAACTCTG TACTGTGAGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000931.1
CDS	26..1090
Translation	MGKLVALVLLGVGLSLVGEMFLAFRERVNASREVEPVEPENCHLIEELESGSEDIDILPSGLAFISSGLKYPGMPNFAPDEPGKIFLMDLNEQNPRAQALEISGGFDKELFNPHGISIFIDKDNTVYLYVVNHPHMKSTVEIFKFEEQQRSLVYLKTIKHELLKSVNDIVVLGPEQFYATRDHYFTNSLLSFFEMILDLRWTYVLFYSPREVKVVAKGFCSANGITVSADQKYVYVADVAAKNIHIMEKHDNWDLTQLKVIQLGTLVDNLTVDPATGDILAGCHPNPMKLLNYNPEDPPGSEVLRIQNVLSEKPRVSTVYANNGSVLQGTSVASVYHGKILIGTVFHKTLYCEL

Target ORF information:

RefSeq Version	NM_000940.2
Organism	Homo sapiens(human)
Definition	Homo sapiens paraoxonase 3 (PON3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000940.2

ORF Insert Sequence:

ATGGGGAAGC TCGTGGCGCT GGTCCTGCTG GGGGTCGGCC TGTCCTTAGT CGGGGAGATG 
TTCCTGGCGT TTAGAGAAAG GGTGAATGCC TCTCGAGAAG TGGAGCCAGT AGAACCTGAA 
AACTGCCACC TTATTGAGGA ACTTGAAAGT GGCTCTGAAG ATATTGATAT ACTTCCTAGT 
GGGCTGGCTT TTATCTCCAG TGGATTAAAA TATCCAGGCA TGCCAAACTT TGCGCCAGAT 
GAACCAGGAA AAATCTTCTT GATGGATCTG AATGAACAAA ACCCAAGGGC ACAAGCGCTA 
GAAATCAGTG GTGGATTTGA CAAAGAATTA TTTAATCCAC ATGGGATCAG TATTTTCATC 
GACAAAGACA ATACTGTGTA TCTTTATGTT GTGAATCATC CCCACATGAA GTCCACTGTG 
GAGATATTTA AATTTGAGGA ACAACAACGT TCTCTGGTAT ACCTGAAAAC TATAAAACAT 
GAACTTCTCA AAAGTGTGAA TGACATTGTG GTTCTTGGAC CAGAACAGTT CTATGCCACC 
AGAGACCACT ATTTTACCAA CTCCCTCCTG TCATTTTTTG AGATGATCTT GGATCTTCGC 
TGGACTTATG TTCTTTTCTA CAGCCCAAGG GAGGTTAAAG TGGTGGCCAA AGGATTTTGT 
AGTGCCAATG GGATCACAGT CTCAGCAGAC CAGAAGTATG TCTATGTAGC TGATGTAGCA 
GCTAAGAACA TTCACATAAT GGAAAAACAT GATAACTGGG ATTTAACTCA ACTGAAGGTG 
ATACAGTTGG GCACCTTAGT GGATAACCTG ACTGTCGATC CTGCCACAGG AGACATTTTG 
GCAGGATGCC ATCCTAATCC TATGAAGCTA CTGAACTATA ACCCTGAGGA CCCTCCAGGA 
TCAGAAGTAC TTCGCATCCA GAATGTTTTG TCTGAGAAGC CCAGGGTGAG CACCGTGTAT 
GCCAACAATG GCTCTGTGCT TCAGGGCACC TCTGTGGCTT CTGTGTACCA TGGGAAAATT 
CTCATAGGCA CCGTATTTCA CAAAACTCTG TACTGTGAGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of lipoic acid on paraoxonase-1 and paraoxonase-3 protein levels, mRNA expression and arylesterase activity in liver hepatoma cells.
General physiology and biophysics36(4)465-470(2017 Oct)
Ozgun E,Sayilan Ozgun G,Tabakcioglu K,Suer Gokmen S,Sut N,Eskiocak S

Paraoxonases and infectious diseases.
Clinical biochemistry50(13-14)804-811(2017 Sep)
Camps J,Iftimie S,Garc?a-Heredia A,Castro A,Joven J

Paraoxonases and psoriasis: negative imbalance of anti--oxidant endogenous mechanisms.
Giornale italiano di dermatologia e venereologia : organo ufficiale, Societa italiana di dermatologia e sifilografia(2017 May)
Schiattarella M,Caiazzo G,DI Caprio R,Lembo S,Raimondo A,Ayala F,Balato N,Monfrecola G,Fortunato G,Balato A

Ocular distribution of antioxidant enzyme paraoxonase & its alteration in cataractous lens & diabetic retina.
The Indian journal of medical research145(4)513-520(2017 Apr)
Bharathidevi SR,Babu KA,Jain N,Muthukumaran S,Umashankar V,Biswas J,Angayarkanni N

Paraoxonase: The Universal Factor of Antioxidant Defense in Human Body.
Vestnik Rossiiskoi akademii meditsinskikh nauk72(1)5-10(2017)
Borovkova EI,Antipova NV,Komeenko TV,Shakhparonov MI,Borovkov IM

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PON3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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