SEMA5B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEMA5B
Entrez Gene ID	54437
Full Name	semaphorin 5B
Synonyms	SEMAG,SemG
General protein information	Preferred Names semaphorin 5B Names semaphorin-5B sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q21.1
Summary	This gene encodes a member of the semaphorin protein family which regulates axon growth during development of the nervous system. The encoded protein has a characteristic Sema domain near the N-terminus, through which semaphorins bind to plexin, and five thrombospondin type 1 repeats in the C-terminal region of the protein. The protein product may be cleaved and exist as a secreted molecule (PMID: 19463192). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012].
Disorder MIM:	609298

mRNA and Protein(s)

mRNA	Protein	Name
NM_001031702.4	NP_001026872.2	semaphorin-5B isoform 1
XM_017006638.2	XP_016862127.1	semaphorin-5B isoform X1
NM_001256347.1	NP_001243276.1	semaphorin-5B isoform 2
NM_001031702.3	NP_001026872.2	semaphorin-5B isoform 1
NM_001256346.1	NP_001243275.1	semaphorin-5B isoform 1
NM_001256348.1	NP_001243277.1	semaphorin-5B isoform 3 precursor
XM_017006638.1	XP_016862127.1	semaphorin-5B isoform X1
XM_017006639.1	XP_016862128.1	semaphorin-5B isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sema5b	NP_001100561.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002424	XP_312521.5
Canis lupus familiaris (dog)	SEMA5B	XP_005639706.1
Bos taurus (cattle)	SEMA5B	XP_002684855.1
Gallus gallus (chicken)	SEMA5B	XP_422096.2
Homo sapiens (human)	SEMA5B	NP_001026872.2
Pan troglodytes (chimpanzee)	SEMA5B	XP_516697.4
Mus musculus (house mouse)	Sema5b	NP_038689.2
	SEMA5B	XP_002802721.1
Danio rerio (zebrafish)	sema5ba	XP_684379.3
Drosophila melanogaster (fruit fly)	Sema-5c	NP_611293.3

Gene Ontology
Bibliography

Related articles in PubMed

Driver Gene and Novel Mutations in Asbestos-Exposed Lung Adenocarcinoma and Malignant Mesothelioma Detected by Exome Sequencing.
M?ki-Nevala S, Sarhadi VK, Knuuttila A, Scheinin I, Ellonen P, Lagstr?m S, R?nty M, Kettunen E, Husgafvel-Pursiainen K, Wolff H, Knuutila S
Lung194(1)125-35(2016 Feb)

Genome-wide association study identifies three new susceptibility loci for esophageal squamous-cell carcinoma in Chinese populations.
Wu C, Hu Z, He Z, Jia W, Wang F, Zhou Y, Liu Z, Zhan Q, Liu Y, Yu D, Zhai K, Chang J, Qiao Y, Jin G, Liu Z, Shen Y, Guo C, Fu J, Miao X, Tan W, Shen H, Ke Y, Zeng Y, Wu T, Lin D
Nature genetics43(7)679-84(2011 Jun)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
Talmud PJ, Drenos F, Shah S, Shah T, Palmen J, Verzilli C, Gaunt TR, Pallas J, Lovering R, Li K, Casas JP, Sofat R, Kumari M, Rodriguez S, Johnson T, Newhouse SJ, Dominiczak A, Samani NJ, Caulfield M, Sever P, Stanton A, Shields DC, Padmanabhan S, Melander O, Hastie C, Delles C, Ebrahim S, Marmot MG, Smith GD, Lawlor DA, Munroe PB, Day IN, Kivimaki M, Whittaker J, Humphries SE, Hingorani AD, , ,
American journal of human genetics85(5)628-42(2009 Nov)

Semaphorin 5B mediates synapse elimination in hippocampal neurons.
O'Connor TP, Cockburn K, Wang W, Tapia L, Currie E, Bamji SX
Neural development418(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SEMA5B could possibly serve as a candidate gene for alterations associated with asbestos exposure.
Title: Driver Gene and Novel Mutations in Asbestos-Exposed Lung Adenocarcinoma and Malignant Mesothelioma Detected by Exome Sequencing.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

Sema5B regulates the development and maintenance of synapse size and number in hippocampal neurons.
Title: Semaphorin 5B mediates synapse elimination in hippocampal neurons.

The following SEMA5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00289	NM_001256347.1 Latest version!	Homo sapiens semaphorin 5B (SEMA5B), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu94293	XM_017006639.1 Latest version!	Homo sapiens semaphorin 5B (SEMA5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu94292	XM_017006638.2 Latest version!	Homo sapiens semaphorin 5B (SEMA5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu00906	NM_001256348.1 Latest version!	Homo sapiens semaphorin 5B (SEMA5B), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu01368	NM_001256346.1 Latest version!	Homo sapiens semaphorin 5B (SEMA5B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu01368	NM_001031702.4 Latest version!	Homo sapiens semaphorin 5B (SEMA5B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
View Old Accession Versions >>
OHu01368	NM_001031702.3	Homo sapiens semaphorin 5B (SEMA5B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
OHu94292	XM_017006638.1	Homo sapiens semaphorin 5B (SEMA5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEMA5B ( NM_001256347.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243276.1 Homo sapiens semaphorin 5B (SEMA5B), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu00289
Clone ID Related Accession (Same CDS sequence)	NM_001256347.1
Accession Version	NM_001256347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Homo sapiens(human)
Product	semaphorin-5B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK295619.1, AC109130.8 and AC078794.13. Transcript Variant: This variant (3) differs in the 5' UTR and coding region and uses an alternate start codon compared to variant 1. The resulting protein (isoform 2) has a longer N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK295619.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCTGCATC TGTCAGCAGA GGAAGCCATT GGCTGCGTGA GGGTCAGAAG GAGCTTCATA 
GATGAGCTGG CATTTGGGAG AGGGCATTCT ACAGGGACGG GCAAGCAGAA GCGAAGGGAT 
CGAGTGAGTG GTTCCAGCTG GTGCCTGGCC TGTGTCTCTT GGATGCCCTG TGGCTTCAGT 
CCGTCTCCTG TTGCCCACCA CCTCGTCCCT GGGCCGCCTG ATACCCCAGC CCAACAGCTA 
AGGTGTGGAT GGACAGTAGG GGGCTGGCTT CTCTCACTGG TCAGGGGTCT TCTCCCCTGT 
CTGCCTCCCG GAGCTAGGAC TGCAGAGGGG CCTATCATGG TGCTTGCAGG CCCCCTGGCT 
GTCTCGCTGT TGCTGCCCAG CCTCACACTG CTGGTGTCCC ACCTCTCCAG CTCCCAGGAT 
GTCTCCAGTG AGCCCAGCAG TGAGCAGCAG CTGTGCGCCC TTAGCAAGCA CCCCACCGTG 
GCCTTTGAAG ACCTGCAGCC GTGGGTCTCT AACTTCACCT ACCCTGGAGC CCGGGATTTC 
TCCCAGCTGG CTTTGGACCC CTCCGGGAAC CAGCTCATCG TGGGAGCCAG GAACTACCTC 
TTCAGACTCA GCCTTGCCAA TGTCTCTCTT CTTCAGGCCA CAGAGTGGGC CTCCAGTGAG 
GACACGCGCC GCTCCTGCCA AAGCAAAGGG AAGACTGAGG AGGAGTGTCA GAACTACGTG 
CGAGTCCTGA TCGTCGCCGG CCGGAAGGTG TTCATGTGTG GAACCAATGC CTTTTCCCCC 
ATGTGCACCA GCAGACAGGT GGGGAACCTC AGCCGGACTA TTGAGAAGAT CAATGGTGTG 
GCCCGCTGCC CCTATGACCC ACGCCACAAC TCCACAGCTG TCATCTCCTC CCAGGGGGAG 
CTCTATGCAG CCACGGTCAT CGACTTCTCA GGTCGGGACC CTGCCATCTA CCGCAGCCTG 
GGCAGTGGGC CACCGCTTCG CACTGCCCAA TATAACTCCA AGTGGCTTAA TGAGCCAAAC 
TTCGTGGCAG CCTATGATAT TGGGCTGTTT GCATACTTCT TCCTGCGGGA GAACGCAGTG 
GAGCACGACT GTGGACGCAC CGTGTACTCT CGCGTGGCCC GCGTGTGCAA GAATGACGTG 
GGGGGCCGAT TCCTGCTGGA GGACACATGG ACCACATTCA TGAAGGCCCG GCTCAACTGC 
TCCCGCCCGG GCGAGGTCCC CTTCTACTAT AACGAGCTGC AGAGTGCCTT CCACTTGCCG 
GAGCAGGACC TCATCTATGG AGTTTTCACA ACCAACGTAA ACAGCATCGC GGCTTCTGCT 
GTCTGCGCCT TCAACCTCAG TGCTATCTCC CAGGCTTTCA ATGGCCCATT TCGCTACCAG 
GAGAACCCCA GGGCTGCCTG GCTCCCCATA GCCAACCCCA TCCCCAATTT CCAGTGTGGC 
ACCCTGCCTG AGACCGGTCC CAACGAGAAC CTGACGGAGC GCAGCCTGCA GGACGCGCAG 
CGCCTCTTCC TGATGAGCGA GGCCGTGCAG CCGGTGACAC CCGAGCCCTG TGTCACCCAG 
GACAGCGTGC GCTTCTCACA CCTCGTGGTG GACCTGGTGC AGGCTAAAGA CACGCTCTAC 
CATGTACTCT ACATTGGCAC CGAGTCGGGC ACCATCCTGA AGGCGCTGTC CACGGCGAGC 
CGCAGCCTCC ACGGCTGCTA CCTGGAGGAG CTGCACGTGC TGCCCCCCGG GCGCCGCGAG 
CCCCTGCGCA GCCTGCGCAT CCTGCACAGC GCCCGCGCGC TCTTCGTGGG GCTGAGAGAC 
GGCGTCCTGC GGGTCCCACT GGAGAGGTGC GCCGCCTACC GCAGCCAGGG GGCATGCCTG 
GGGGCCCGGG ACCCGTACTG TGGCTGGGAC GGGAAGCAGC AACGTTGCAG CACACTCGAG 
GACAGCTCCA ACATGAGCCT CTGGACCCAG AACATCACCG CCTGTCCTGT GCGGAATGTG 
ACACGGGATG GGGGCTTCGG CCCATGGTCA CCATGGCAAC CATGTGAGCA CTTGGATGGG 
GACAACTCAG GCTCTTGCCT GTGTCGAGCT CGATCCTGTG ATTCCCCTCG ACCCCGCTGT 
GGGGGCCTTG ACTGCCTGGG GCCAGCCATC CACATCGCCA ACTGCTCCAG GAATGGGGCG 
TGGACCCCGT GGTCATCGTG GGCGCTGTGC AGCACGTCCT GTGGCATCGG CTTCCAGGTC 
CGCCAGCGAA GTTGCAGCAA CCCTGCTCCC CGCCACGGGG GCCGCATCTG CGTGGGCAAG 
AGCCGGGAGG AACGGTTCTG TAATGAGAAC ACGCCTTGCC CGGTGCCCAT CTTCTGGGCT 
TCCTGGGGCT CCTGGAGCAA GTGCAGCAGC AACTGTGGAG GGGGCATGCA GTCGCGGCGT 
CGGGCCTGCG AGAACGGCAA CTCCTGCCTG GGCTGCGGCG TGGAGTTCAA GACGTGCAAC 
CCCGAGGGCT GCCCCGAAGT GCGGCGCAAC ACCCCCTGGA CGCCGTGGCT GCCCGTGAAC 
GTGACGCAGG GCGGGGCACG GCAGGAGCAG CGGTTCCGCT TCACCTGCCG CGCGCCCCTT 
GCAGACCCGC ACGGCCTGCA GTTCGGCAGG AGAAGGACCG AGACGAGGAC CTGTCCCGCG 
GACGGCTCCG GCTCCTGCGA CACCGACGCC CTGGTGGAGG TCCTCCTGCG CAGCGGGAGC 
ACCTCCCCGC ACACGGTGAG CGGGGGCTGG GCCGCCTGGG GCCCGTGGTC GTCCTGCTCC 
CGGGACTGCG AGCTGGGCTT CCGCGTCCGC AAGAGAACGT GCACTAACCC GGAGCCCCGC 
AACGGGGGCC TGCCCTGCGT GGGCGATGCT GCCGAGTACC AGGACTGCAA CCCCCAGGCT 
TGCCCAGTTC GGGGTGCTTG GTCCTGCTGG ACCTCATGGT CTCCATGCTC AGCTTCCTGT 
GGTGGGGGTC ACTATCAACG CACCCGTTCC TGCACCAGCC CCGCACCCTC CCCAGGTGAG 
GACATCTGTC TCGGGCTGCA CACGGAGGAG GCACTATGTG CCACACAGGC CTGCCCAGAA 
GGCTGGTCGC CCTGGTCTGA GTGGAGTAAG TGCACTGACG ACGGAGCCCA GAGCCGAAGC 
CGGCACTGTG AGGAGCTCCT CCCAGGGTCC AGCGCCTGTG CTGGAAACAG CAGCCAGAGC 
CGCCCCTGCC CCTACAGCGA GATTCCCGTC ATCCTGCCAG CCTCCAGCAT GGAGGAGGCC 
ACCGACTGTG CAGGGTTCAA TCTCATCCAC TTGGTGGCCA CGGGCATCTC CTGCTTCTTG 
GGCTCTGGGC TCCTGACCCT AGCAGTGTAC CTGTCTTGCC AGCACTGCCA GCGTCAGTCC 
CAGGAGTCCA CACTGGTCCA TCCTGCCACC CCCAACCATT TGCACTACAA GGGCGGAGGC 
ACCCCGAAGA ATGAAAAGTA CACACCCATG GAATTCAAGA CCCTGAACAA GAATAACTTG 
ATCCCTGATG ACAGAGCCAA CTTCTACCCA TTGCAGCAGA CCAATGTGTA CACGACTACT 
TACTACCCAA GCCCCCTGAA CAAACACAGC TTCCGGCCCG AGGCCTCACC TGGACAACGG 
TGCTTCCCCA ACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243276.1
CDS	12..3629
Translation	MLHLSAEEAIGCVRVRRSFIDELAFGRGHSTGTGKQKRRDRVSGSSWCLACVSWMPCGFSPSPVAHHLVPGPPDTPAQQLRCGWTVGGWLLSLVRGLLPCLPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCNPEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGWAAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIHLVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	NM_001256347.1
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 5B (SEMA5B), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256347.1

ORF Insert Sequence:

ATGCTGCATC TGTCAGCAGA GGAAGCCATT GGCTGCGTGA GGGTCAGAAG GAGCTTCATA 
GATGAGCTGG CATTTGGGAG AGGGCATTCT ACAGGGACGG GCAAGCAGAA GCGAAGGGAT 
CGAGTGAGTG GTTCCAGCTG GTGCCTGGCC TGTGTCTCTT GGATGCCCTG TGGCTTCAGT 
CCGTCTCCTG TTGCCCACCA CCTCGTCCCT GGGCCGCCTG ATACCCCAGC CCAACAGCTA 
AGGTGTGGAT GGACAGTAGG GGGCTGGCTT CTCTCACTGG TCAGGGGTCT TCTCCCCTGT 
CTGCCTCCCG GAGCTAGGAC TGCAGAGGGG CCTATCATGG TGCTTGCAGG CCCCCTGGCT 
GTCTCGCTGT TGCTGCCCAG CCTCACACTG CTGGTGTCCC ACCTCTCCAG CTCCCAGGAT 
GTCTCCAGTG AGCCCAGCAG TGAGCAGCAG CTGTGCGCCC TTAGCAAGCA CCCCACCGTG 
GCCTTTGAAG ACCTGCAGCC GTGGGTCTCT AACTTCACCT ACCCTGGAGC CCGGGATTTC 
TCCCAGCTGG CTTTGGACCC CTCCGGGAAC CAGCTCATCG TGGGAGCCAG GAACTACCTC 
TTCAGACTCA GCCTTGCCAA TGTCTCTCTT CTTCAGGCCA CAGAGTGGGC CTCCAGTGAG 
GACACGCGCC GCTCCTGCCA AAGCAAAGGG AAGACTGAGG AGGAGTGTCA GAACTACGTG 
CGAGTCCTGA TCGTCGCCGG CCGGAAGGTG TTCATGTGTG GAACCAATGC CTTTTCCCCC 
ATGTGCACCA GCAGACAGGT GGGGAACCTC AGCCGGACTA TTGAGAAGAT CAATGGTGTG 
GCCCGCTGCC CCTATGACCC ACGCCACAAC TCCACAGCTG TCATCTCCTC CCAGGGGGAG 
CTCTATGCAG CCACGGTCAT CGACTTCTCA GGTCGGGACC CTGCCATCTA CCGCAGCCTG 
GGCAGTGGGC CACCGCTTCG CACTGCCCAA TATAACTCCA AGTGGCTTAA TGAGCCAAAC 
TTCGTGGCAG CCTATGATAT TGGGCTGTTT GCATACTTCT TCCTGCGGGA GAACGCAGTG 
GAGCACGACT GTGGACGCAC CGTGTACTCT CGCGTGGCCC GCGTGTGCAA GAATGACGTG 
GGGGGCCGAT TCCTGCTGGA GGACACATGG ACCACATTCA TGAAGGCCCG GCTCAACTGC 
TCCCGCCCGG GCGAGGTCCC CTTCTACTAT AACGAGCTGC AGAGTGCCTT CCACTTGCCG 
GAGCAGGACC TCATCTATGG AGTTTTCACA ACCAACGTAA ACAGCATCGC GGCTTCTGCT 
GTCTGCGCCT TCAACCTCAG TGCTATCTCC CAGGCTTTCA ATGGCCCATT TCGCTACCAG 
GAGAACCCCA GGGCTGCCTG GCTCCCCATA GCCAACCCCA TCCCCAATTT CCAGTGTGGC 
ACCCTGCCTG AGACCGGTCC CAACGAGAAC CTGACGGAGC GCAGCCTGCA GGACGCGCAG 
CGCCTCTTCC TGATGAGCGA GGCCGTGCAG CCGGTGACAC CCGAGCCCTG TGTCACCCAG 
GACAGCGTGC GCTTCTCACA CCTCGTGGTG GACCTGGTGC AGGCTAAAGA CACGCTCTAC 
CATGTACTCT ACATTGGCAC CGAGTCGGGC ACCATCCTGA AGGCGCTGTC CACGGCGAGC 
CGCAGCCTCC ACGGCTGCTA CCTGGAGGAG CTGCACGTGC TGCCCCCCGG GCGCCGCGAG 
CCCCTGCGCA GCCTGCGCAT CCTGCACAGC GCCCGCGCGC TCTTCGTGGG GCTGAGAGAC 
GGCGTCCTGC GGGTCCCACT GGAGAGGTGC GCCGCCTACC GCAGCCAGGG GGCATGCCTG 
GGGGCCCGGG ACCCGTACTG TGGCTGGGAC GGGAAGCAGC AACGTTGCAG CACACTCGAG 
GACAGCTCCA ACATGAGCCT CTGGACCCAG AACATCACCG CCTGTCCTGT GCGGAATGTG 
ACACGGGATG GGGGCTTCGG CCCATGGTCA CCATGGCAAC CATGTGAGCA CTTGGATGGG 
GACAACTCAG GCTCTTGCCT GTGTCGAGCT CGATCCTGTG ATTCCCCTCG ACCCCGCTGT 
GGGGGCCTTG ACTGCCTGGG GCCAGCCATC CACATCGCCA ACTGCTCCAG GAATGGGGCG 
TGGACCCCGT GGTCATCGTG GGCGCTGTGC AGCACGTCCT GTGGCATCGG CTTCCAGGTC 
CGCCAGCGAA GTTGCAGCAA CCCTGCTCCC CGCCACGGGG GCCGCATCTG CGTGGGCAAG 
AGCCGGGAGG AACGGTTCTG TAATGAGAAC ACGCCTTGCC CGGTGCCCAT CTTCTGGGCT 
TCCTGGGGCT CCTGGAGCAA GTGCAGCAGC AACTGTGGAG GGGGCATGCA GTCGCGGCGT 
CGGGCCTGCG AGAACGGCAA CTCCTGCCTG GGCTGCGGCG TGGAGTTCAA GACGTGCAAC 
CCCGAGGGCT GCCCCGAAGT GCGGCGCAAC ACCCCCTGGA CGCCGTGGCT GCCCGTGAAC 
GTGACGCAGG GCGGGGCACG GCAGGAGCAG CGGTTCCGCT TCACCTGCCG CGCGCCCCTT 
GCAGACCCGC ACGGCCTGCA GTTCGGCAGG AGAAGGACCG AGACGAGGAC CTGTCCCGCG 
GACGGCTCCG GCTCCTGCGA CACCGACGCC CTGGTGGAGG TCCTCCTGCG CAGCGGGAGC 
ACCTCCCCGC ACACGGTGAG CGGGGGCTGG GCCGCCTGGG GCCCGTGGTC GTCCTGCTCC 
CGGGACTGCG AGCTGGGCTT CCGCGTCCGC AAGAGAACGT GCACTAACCC GGAGCCCCGC 
AACGGGGGCC TGCCCTGCGT GGGCGATGCT GCCGAGTACC AGGACTGCAA CCCCCAGGCT 
TGCCCAGTTC GGGGTGCTTG GTCCTGCTGG ACCTCATGGT CTCCATGCTC AGCTTCCTGT 
GGTGGGGGTC ACTATCAACG CACCCGTTCC TGCACCAGCC CCGCACCCTC CCCAGGTGAG 
GACATCTGTC TCGGGCTGCA CACGGAGGAG GCACTATGTG CCACACAGGC CTGCCCAGAA 
GGCTGGTCGC CCTGGTCTGA GTGGAGTAAG TGCACTGACG ACGGAGCCCA GAGCCGAAGC 
CGGCACTGTG AGGAGCTCCT CCCAGGGTCC AGCGCCTGTG CTGGAAACAG CAGCCAGAGC 
CGCCCCTGCC CCTACAGCGA GATTCCCGTC ATCCTGCCAG CCTCCAGCAT GGAGGAGGCC 
ACCGACTGTG CAGGGTTCAA TCTCATCCAC TTGGTGGCCA CGGGCATCTC CTGCTTCTTG 
GGCTCTGGGC TCCTGACCCT AGCAGTGTAC CTGTCTTGCC AGCACTGCCA GCGTCAGTCC 
CAGGAGTCCA CACTGGTCCA TCCTGCCACC CCCAACCATT TGCACTACAA GGGCGGAGGC 
ACCCCGAAGA ATGAAAAGTA CACACCCATG GAATTCAAGA CCCTGAACAA GAATAACTTG 
ATCCCTGATG ACAGAGCCAA CTTCTACCCA TTGCAGCAGA CCAATGTGTA CACGACTACT 
TACTACCCAA GCCCCCTGAA CAAACACAGC TTCCGGCCCG AGGCCTCACC TGGACAACGG 
TGCTTCCCCA ACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5B ( XM_017006639.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862128.1 Homo sapiens semaphorin 5B (SEMA5B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu94293
Clone ID Related Accession (Same CDS sequence)	XM_017006639.1
Accession Version	XM_017006639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	semaphorin-5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC 
GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC 
ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG 
GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT 
GAGGAGGAGT GTCAGAACTA CGTGCGAGTC CTGATCGTCG CCGGCCGGAA GGTGTTCATG 
TGTGGAACCA ATGCCTTTTC CCCCATGTGC ACCAGCAGAC AGGTGGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAATGG TGTGGCCCGC TGCCCCTATG ACCCACGCCA CAACTCCACA 
GCTGTCATCT CCTCCCAGGG GGAGCTCTAT GCAGCCACGG TCATCGACTT CTCAGGTCGG 
GACCCTGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAATATAAC 
TCCAAGTGGC TTAATGAGCC AAACTTCGTG GCAGCCTATG ATATTGGGCT GTTTGCATAC 
TTCTTCCTGC GGGAGAACGC AGTGGAGCAC GACTGTGGAC GCACCGTGTA CTCTCGCGTG 
GCCCGCGTGT GCAAGAATGA CGTGGGGGGC CGATTCCTGC TGGAGGACAC ATGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGCGAGG TCCCCTTCTA CTATAACGAG 
CTGCAGAGTG CCTTCCACTT GCCGGAGCAG GACCTCATCT ATGGAGTTTT CACAACCAAC 
GTAAACAGCA TCGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCTAT CTCCCAGGCT 
TTCAATGGCC CATTTCGCTA CCAGGAGAAC CCCAGGGCTG CCTGGCTCCC CATAGCCAAC 
CCCATCCCCA ATTTCCAGTG TGGCACCCTG CCTGAGACCG GTCCCAACGA GAACCTGACG 
GAGCGCAGCC TGCAGGACGC GCAGCGCCTC TTCCTGATGA GCGAGGCCGT GCAGCCGGTG 
ACACCCGAGC CCTGTGTCAC CCAGGACAGC GTGCGCTTCT CACACCTCGT GGTGGACCTG 
GTGCAGGCTA AAGACACGCT CTACCATGTA CTCTACATTG GCACCGAGTC GGGCACCATC 
CTGAAGGCGC TGTCCACGGC GAGCCGCAGC CTCCACGGCT GCTACCTGGA GGAGCTGCAC 
GTGCTGCCCC CCGGGCGCCG CGAGCCCCTG CGCAGCCTGC GCATCCTGCA CAGCGCCCGC 
GCGCTCTTCG TGGGGCTGAG AGACGGCGTC CTGCGGGTCC CACTGGAGAG GTGCGCCGCC 
TACCGCAGCC AGGGGGCATG CCTGGGGGCC CGGGACCCGT ACTGTGGCTG GGACGGGAAG 
CAGCAACGTT GCAGCACACT CGAGGACAGC TCCAACATGA GCCTCTGGAC CCAGAACATC 
ACCGCCTGTC CTGTGCGGAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
CAACCATGTG AGCACTTGGA TGGGGACAAC TCAGGCTCTT GCCTGTGTCG AGCTCGATCC 
TGTGATTCCC CTCGACCCCG CTGTGGGGGC CTTGACTGCC TGGGGCCAGC CATCCACATC 
GCCAACTGCT CCAGGAATGG GGCGTGGACC CCGTGGTCAT CGTGGGCGCT GTGCAGCACG 
TCCTGTGGCA TCGGCTTCCA GGTCCGCCAG CGAAGTTGCA GCAACCCTGC TCCCCGCCAC 
GGGGGCCGCA TCTGCGTGGG CAAGAGCCGG GAGGAACGGT TCTGTAATGA GAACACGCCT 
TGCCCGGTGC CCATCTTCTG GGCTTCCTGG GGCTCCTGGA GCAAGTGCAG CAGCAACTGT 
GGAGGGGGCA TGCAGTCGCG GCGTCGGGCC TGCGAGAACG GCAACTCCTG CCTGGGCTGC 
GGCGTGGAGT TCAAGACGTG CAACCCCGAG GGCTGCCCCG AAGTGCGGCG CAACACCCCC 
TGGACGCCGT GGCTGCCCGT GAACGTGACG CAGGGCGGGG CACGGCAGGA GCAGCGGTTC 
CGCTTCACCT GCCGCGCGCC CCTTGCAGAC CCGCACGGCC TGCAGTTCGG CAGGAGAAGG 
ACCGAGACGA GGACCTGTCC CGCGGACGGC TCCGGCTCCT GCGACACCGA CGGTACCCCG 
CGCCCGGCGC CCCCTCCTTC CTCTCTCCCA GCCCCGGAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862128.1
CDS	354..2738
Translation	MVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCNPEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDGTPRPAPPPSSLPAPER

Target ORF information:

RefSeq Version	XM_017006639.1
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 5B (SEMA5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006639.1

ORF Insert Sequence:

ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC 
GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC 
ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG 
GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT 
GAGGAGGAGT GTCAGAACTA CGTGCGAGTC CTGATCGTCG CCGGCCGGAA GGTGTTCATG 
TGTGGAACCA ATGCCTTTTC CCCCATGTGC ACCAGCAGAC AGGTGGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAATGG TGTGGCCCGC TGCCCCTATG ACCCACGCCA CAACTCCACA 
GCTGTCATCT CCTCCCAGGG GGAGCTCTAT GCAGCCACGG TCATCGACTT CTCAGGTCGG 
GACCCTGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAATATAAC 
TCCAAGTGGC TTAATGAGCC AAACTTCGTG GCAGCCTATG ATATTGGGCT GTTTGCATAC 
TTCTTCCTGC GGGAGAACGC AGTGGAGCAC GACTGTGGAC GCACCGTGTA CTCTCGCGTG 
GCCCGCGTGT GCAAGAATGA CGTGGGGGGC CGATTCCTGC TGGAGGACAC ATGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGCGAGG TCCCCTTCTA CTATAACGAG 
CTGCAGAGTG CCTTCCACTT GCCGGAGCAG GACCTCATCT ATGGAGTTTT CACAACCAAC 
GTAAACAGCA TCGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCTAT CTCCCAGGCT 
TTCAATGGCC CATTTCGCTA CCAGGAGAAC CCCAGGGCTG CCTGGCTCCC CATAGCCAAC 
CCCATCCCCA ATTTCCAGTG TGGCACCCTG CCTGAGACCG GTCCCAACGA GAACCTGACG 
GAGCGCAGCC TGCAGGACGC GCAGCGCCTC TTCCTGATGA GCGAGGCCGT GCAGCCGGTG 
ACACCCGAGC CCTGTGTCAC CCAGGACAGC GTGCGCTTCT CACACCTCGT GGTGGACCTG 
GTGCAGGCTA AAGACACGCT CTACCATGTA CTCTACATTG GCACCGAGTC GGGCACCATC 
CTGAAGGCGC TGTCCACGGC GAGCCGCAGC CTCCACGGCT GCTACCTGGA GGAGCTGCAC 
GTGCTGCCCC CCGGGCGCCG CGAGCCCCTG CGCAGCCTGC GCATCCTGCA CAGCGCCCGC 
GCGCTCTTCG TGGGGCTGAG AGACGGCGTC CTGCGGGTCC CACTGGAGAG GTGCGCCGCC 
TACCGCAGCC AGGGGGCATG CCTGGGGGCC CGGGACCCGT ACTGTGGCTG GGACGGGAAG 
CAGCAACGTT GCAGCACACT CGAGGACAGC TCCAACATGA GCCTCTGGAC CCAGAACATC 
ACCGCCTGTC CTGTGCGGAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
CAACCATGTG AGCACTTGGA TGGGGACAAC TCAGGCTCTT GCCTGTGTCG AGCTCGATCC 
TGTGATTCCC CTCGACCCCG CTGTGGGGGC CTTGACTGCC TGGGGCCAGC CATCCACATC 
GCCAACTGCT CCAGGAATGG GGCGTGGACC CCGTGGTCAT CGTGGGCGCT GTGCAGCACG 
TCCTGTGGCA TCGGCTTCCA GGTCCGCCAG CGAAGTTGCA GCAACCCTGC TCCCCGCCAC 
GGGGGCCGCA TCTGCGTGGG CAAGAGCCGG GAGGAACGGT TCTGTAATGA GAACACGCCT 
TGCCCGGTGC CCATCTTCTG GGCTTCCTGG GGCTCCTGGA GCAAGTGCAG CAGCAACTGT 
GGAGGGGGCA TGCAGTCGCG GCGTCGGGCC TGCGAGAACG GCAACTCCTG CCTGGGCTGC 
GGCGTGGAGT TCAAGACGTG CAACCCCGAG GGCTGCCCCG AAGTGCGGCG CAACACCCCC 
TGGACGCCGT GGCTGCCCGT GAACGTGACG CAGGGCGGGG CACGGCAGGA GCAGCGGTTC 
CGCTTCACCT GCCGCGCGCC CCTTGCAGAC CCGCACGGCC TGCAGTTCGG CAGGAGAAGG 
ACCGAGACGA GGACCTGTCC CGCGGACGGC TCCGGCTCCT GCGACACCGA CGGTACCCCG 
CGCCCGGCGC CCCCTCCTTC CTCTCTCCCA GCCCCGGAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5B ( XM_017006638.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862127.1 Homo sapiens semaphorin 5B (SEMA5B), transcript variant X1, mRNA.

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CloneID	OHu94292
Clone ID Related Accession (Same CDS sequence)	XM_017006638.2 , XM_017006638.1
Accession Version	XM_017006638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	semaphorin-5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017006638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC 
GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC 
ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG 
GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT 
GAGTGGTGTG AGCGTGAGAT ATCCAGCATC GCACCTGGAG AGCTGTGCTG TCTGCTCCTT 
TCCTTCCTGC CTCAGGAGGA GTGTCAGAAC TACGTGCGAG TCCTGATCGT CGCCGGCCGG 
AAGGTGTTCA TGTGTGGAAC CAATGCCTTT TCCCCCATGT GCACCAGCAG ACAGGTGGGG 
AACCTCAGCC GGACTATTGA GAAGATCAAT GGTGTGGCCC GCTGCCCCTA TGACCCACGC 
CACAACTCCA CAGCTGTCAT CTCCTCCCAG GGGGAGCTCT ATGCAGCCAC GGTCATCGAC 
TTCTCAGGTC GGGACCCTGC CATCTACCGC AGCCTGGGCA GTGGGCCACC GCTTCGCACT 
GCCCAATATA ACTCCAAGTG GCTTAATGAG CCAAACTTCG TGGCAGCCTA TGATATTGGG 
CTGTTTGCAT ACTTCTTCCT GCGGGAGAAC GCAGTGGAGC ACGACTGTGG ACGCACCGTG 
TACTCTCGCG TGGCCCGCGT GTGCAAGAAT GACGTGGGGG GCCGATTCCT GCTGGAGGAC 
ACATGGACCA CATTCATGAA GGCCCGGCTC AACTGCTCCC GCCCGGGCGA GGTCCCCTTC 
TACTATAACG AGCTGCAGAG TGCCTTCCAC TTGCCGGAGC AGGACCTCAT CTATGGAGTT 
TTCACAACCA ACGTAAACAG CATCGCGGCT TCTGCTGTCT GCGCCTTCAA CCTCAGTGCT 
ATCTCCCAGG CTTTCAATGG CCCATTTCGC TACCAGGAGA ACCCCAGGGC TGCCTGGCTC 
CCCATAGCCA ACCCCATCCC CAATTTCCAG TGTGGCACCC TGCCTGAGAC CGGTCCCAAC 
GAGAACCTGA CGGAGCGCAG CCTGCAGGAC GCGCAGCGCC TCTTCCTGAT GAGCGAGGCC 
GTGCAGCCGG TGACACCCGA GCCCTGTGTC ACCCAGGACA GCGTGCGCTT CTCACACCTC 
GTGGTGGACC TGGTGCAGGC TAAAGACACG CTCTACCATG TACTCTACAT TGGCACCGAG 
TCGGGCACCA TCCTGAAGGC GCTGTCCACG GCGAGCCGCA GCCTCCACGG CTGCTACCTG 
GAGGAGCTGC ACGTGCTGCC CCCCGGGCGC CGCGAGCCCC TGCGCAGCCT GCGCATCCTG 
CACAGCGCCC GCGCGCTCTT CGTGGGGCTG AGAGACGGCG TCCTGCGGGT CCCACTGGAG 
AGGTGCGCCG CCTACCGCAG CCAGGGGGCA TGCCTGGGGG CCCGGGACCC GTACTGTGGC 
TGGGACGGGA AGCAGCAACG TTGCAGCACA CTCGAGGACA GCTCCAACAT GAGCCTCTGG 
ACCCAGAACA TCACCGCCTG TCCTGTGCGG AATGTGACAC GGGATGGGGG CTTCGGCCCA 
TGGTCACCAT GGCAACCATG TGAGCACTTG GATGGGGACA ACTCAGGCTC TTGCCTGTGT 
CGAGCTCGAT CCTGTGATTC CCCTCGACCC CGCTGTGGGG GCCTTGACTG CCTGGGGCCA 
GCCATCCACA TCGCCAACTG CTCCAGGAAT GGGGCGTGGA CCCCGTGGTC ATCGTGGGCG 
CTGTGCAGCA CGTCCTGTGG CATCGGCTTC CAGGTCCGCC AGCGAAGTTG CAGCAACCCT 
GCTCCCCGCC ACGGGGGCCG CATCTGCGTG GGCAAGAGCC GGGAGGAACG GTTCTGTAAT 
GAGAACACGC CTTGCCCGGT GCCCATCTTC TGGGCTTCCT GGGGCTCCTG GAGCAAGTGC 
AGCAGCAACT GTGGAGGGGG CATGCAGTCG CGGCGTCGGG CCTGCGAGAA CGGCAACTCC 
TGCCTGGGCT GCGGCGTGGA GTTCAAGACG TGCAACCCCG AGGGCTGCCC CGAAGTGCGG 
CGCAACACCC CCTGGACGCC GTGGCTGCCC GTGAACGTGA CGCAGGGCGG GGCACGGCAG 
GAGCAGCGGT TCCGCTTCAC CTGCCGCGCG CCCCTTGCAG ACCCGCACGG CCTGCAGTTC 
GGCAGGAGAA GGACCGAGAC GAGGACCTGT CCCGCGGACG GCTCCGGCTC CTGCGACACC 
GACGCCCTGG TGGAGGTCCT CCTGCGCAGC GGGAGCACCT CCCCGCACAC GGTGAGCGGG 
GGCTGGGCCG CCTGGGGCCC GTGGTCGTCC TGCTCCCGGG ACTGCGAGCT GGGCTTCCGC 
GTCCGCAAGA GAACGTGCAC TAACCCGGAG CCCCGCAACG GGGGCCTGCC CTGCGTGGGC 
GATGCTGCCG AGTACCAGGA CTGCAACCCC CAGGCTTGCC CAGTTCGGGG TGCTTGGTCC 
TGCTGGACCT CATGGTCTCC ATGCTCAGCT TCCTGTGGTG GGGGTCACTA TCAACGCACC 
CGTTCCTGCA CCAGCCCCGC ACCCTCCCCA GGTGAGGACA TCTGTCTCGG GCTGCACACG 
GAGGAGGCAC TATGTGCCAC ACAGGCCTGC CCAGAAGGCT GGTCGCCCTG GTCTGAGTGG 
AGTAAGTGCA CTGACGACGG AGCCCAGAGC CGAAGCCGGC ACTGTGAGGA GCTCCTCCCA 
GGGTCCAGCG CCTGTGCTGG AAACAGCAGC CAGAGCCGCC CCTGCCCCTA CAGCGAGATT 
CCCGTCATCC TGCCAGCCTC CAGCATGGAG GAGGCCACCG ACTGTGCAGG GTTCAATCTC 
ATCCACTTGG TGGCCACGGG CATCTCCTGC TTCTTGGGCT CTGGGCTCCT GACCCTAGCA 
GTGTACCTGT CTTGCCAGCA CTGCCAGCGT CAGTCCCAGG AGTCCACACT GGTCCATCCT 
GCCACCCCCA ACCATTTGCA CTACAAGGGC GGAGGCACCC CGAAGAATGA AAAGTACACA 
CCCATGGAAT TCAAGACCCT GAACAAGAAT AACTTGATCC CTGATGACAG AGCCAACTTC 
TACCCATTGC AGCAGACCAA TGTGTACACG ACTACTTACT ACCCAAGCCC CCTGAACAAA 
CACAGCTTCC GGCCCGAGGC CTCACCTGGA CAACGGTGCT TCCCCAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862127.1
CDS	1156..4509
Translation	MVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEWCEREISSIAPGELCCLLLSFLPQEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCNPEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGWAAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIHLVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	XM_017006638.2
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 5B (SEMA5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006638.2

ORF Insert Sequence:

ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC 
GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC 
ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG 
GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT 
GAGTGGTGTG AGCGTGAGAT ATCCAGCATC GCACCTGGAG AGCTGTGCTG TCTGCTCCTT 
TCCTTCCTGC CTCAGGAGGA GTGTCAGAAC TACGTGCGAG TCCTGATCGT CGCCGGCCGG 
AAGGTGTTCA TGTGTGGAAC CAATGCCTTT TCCCCCATGT GCACCAGCAG ACAGGTGGGG 
AACCTCAGCC GGACTATTGA GAAGATCAAT GGTGTGGCCC GCTGCCCCTA TGACCCACGC 
CACAACTCCA CAGCTGTCAT CTCCTCCCAG GGGGAGCTCT ATGCAGCCAC GGTCATCGAC 
TTCTCAGGTC GGGACCCTGC CATCTACCGC AGCCTGGGCA GTGGGCCACC GCTTCGCACT 
GCCCAATATA ACTCCAAGTG GCTTAATGAG CCAAACTTCG TGGCAGCCTA TGATATTGGG 
CTGTTTGCAT ACTTCTTCCT GCGGGAGAAC GCAGTGGAGC ACGACTGTGG ACGCACCGTG 
TACTCTCGCG TGGCCCGCGT GTGCAAGAAT GACGTGGGGG GCCGATTCCT GCTGGAGGAC 
ACATGGACCA CATTCATGAA GGCCCGGCTC AACTGCTCCC GCCCGGGCGA GGTCCCCTTC 
TACTATAACG AGCTGCAGAG TGCCTTCCAC TTGCCGGAGC AGGACCTCAT CTATGGAGTT 
TTCACAACCA ACGTAAACAG CATCGCGGCT TCTGCTGTCT GCGCCTTCAA CCTCAGTGCT 
ATCTCCCAGG CTTTCAATGG CCCATTTCGC TACCAGGAGA ACCCCAGGGC TGCCTGGCTC 
CCCATAGCCA ACCCCATCCC CAATTTCCAG TGTGGCACCC TGCCTGAGAC CGGTCCCAAC 
GAGAACCTGA CGGAGCGCAG CCTGCAGGAC GCGCAGCGCC TCTTCCTGAT GAGCGAGGCC 
GTGCAGCCGG TGACACCCGA GCCCTGTGTC ACCCAGGACA GCGTGCGCTT CTCACACCTC 
GTGGTGGACC TGGTGCAGGC TAAAGACACG CTCTACCATG TACTCTACAT TGGCACCGAG 
TCGGGCACCA TCCTGAAGGC GCTGTCCACG GCGAGCCGCA GCCTCCACGG CTGCTACCTG 
GAGGAGCTGC ACGTGCTGCC CCCCGGGCGC CGCGAGCCCC TGCGCAGCCT GCGCATCCTG 
CACAGCGCCC GCGCGCTCTT CGTGGGGCTG AGAGACGGCG TCCTGCGGGT CCCACTGGAG 
AGGTGCGCCG CCTACCGCAG CCAGGGGGCA TGCCTGGGGG CCCGGGACCC GTACTGTGGC 
TGGGACGGGA AGCAGCAACG TTGCAGCACA CTCGAGGACA GCTCCAACAT GAGCCTCTGG 
ACCCAGAACA TCACCGCCTG TCCTGTGCGG AATGTGACAC GGGATGGGGG CTTCGGCCCA 
TGGTCACCAT GGCAACCATG TGAGCACTTG GATGGGGACA ACTCAGGCTC TTGCCTGTGT 
CGAGCTCGAT CCTGTGATTC CCCTCGACCC CGCTGTGGGG GCCTTGACTG CCTGGGGCCA 
GCCATCCACA TCGCCAACTG CTCCAGGAAT GGGGCGTGGA CCCCGTGGTC ATCGTGGGCG 
CTGTGCAGCA CGTCCTGTGG CATCGGCTTC CAGGTCCGCC AGCGAAGTTG CAGCAACCCT 
GCTCCCCGCC ACGGGGGCCG CATCTGCGTG GGCAAGAGCC GGGAGGAACG GTTCTGTAAT 
GAGAACACGC CTTGCCCGGT GCCCATCTTC TGGGCTTCCT GGGGCTCCTG GAGCAAGTGC 
AGCAGCAACT GTGGAGGGGG CATGCAGTCG CGGCGTCGGG CCTGCGAGAA CGGCAACTCC 
TGCCTGGGCT GCGGCGTGGA GTTCAAGACG TGCAACCCCG AGGGCTGCCC CGAAGTGCGG 
CGCAACACCC CCTGGACGCC GTGGCTGCCC GTGAACGTGA CGCAGGGCGG GGCACGGCAG 
GAGCAGCGGT TCCGCTTCAC CTGCCGCGCG CCCCTTGCAG ACCCGCACGG CCTGCAGTTC 
GGCAGGAGAA GGACCGAGAC GAGGACCTGT CCCGCGGACG GCTCCGGCTC CTGCGACACC 
GACGCCCTGG TGGAGGTCCT CCTGCGCAGC GGGAGCACCT CCCCGCACAC GGTGAGCGGG 
GGCTGGGCCG CCTGGGGCCC GTGGTCGTCC TGCTCCCGGG ACTGCGAGCT GGGCTTCCGC 
GTCCGCAAGA GAACGTGCAC TAACCCGGAG CCCCGCAACG GGGGCCTGCC CTGCGTGGGC 
GATGCTGCCG AGTACCAGGA CTGCAACCCC CAGGCTTGCC CAGTTCGGGG TGCTTGGTCC 
TGCTGGACCT CATGGTCTCC ATGCTCAGCT TCCTGTGGTG GGGGTCACTA TCAACGCACC 
CGTTCCTGCA CCAGCCCCGC ACCCTCCCCA GGTGAGGACA TCTGTCTCGG GCTGCACACG 
GAGGAGGCAC TATGTGCCAC ACAGGCCTGC CCAGAAGGCT GGTCGCCCTG GTCTGAGTGG 
AGTAAGTGCA CTGACGACGG AGCCCAGAGC CGAAGCCGGC ACTGTGAGGA GCTCCTCCCA 
GGGTCCAGCG CCTGTGCTGG AAACAGCAGC CAGAGCCGCC CCTGCCCCTA CAGCGAGATT 
CCCGTCATCC TGCCAGCCTC CAGCATGGAG GAGGCCACCG ACTGTGCAGG GTTCAATCTC 
ATCCACTTGG TGGCCACGGG CATCTCCTGC TTCTTGGGCT CTGGGCTCCT GACCCTAGCA 
GTGTACCTGT CTTGCCAGCA CTGCCAGCGT CAGTCCCAGG AGTCCACACT GGTCCATCCT 
GCCACCCCCA ACCATTTGCA CTACAAGGGC GGAGGCACCC CGAAGAATGA AAAGTACACA 
CCCATGGAAT TCAAGACCCT GAACAAGAAT AACTTGATCC CTGATGACAG AGCCAACTTC 
TACCCATTGC AGCAGACCAA TGTGTACACG ACTACTTACT ACCCAAGCCC CCTGAACAAA 
CACAGCTTCC GGCCCGAGGC CTCACCTGGA CAACGGTGCT TCCCCAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5B ( XM_017006638.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862127.1 Homo sapiens semaphorin 5B (SEMA5B), transcript variant X1, mRNA.

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CloneID	OHu94292
Clone ID Related Accession (Same CDS sequence)	XM_017006638.2 , XM_017006638.1
Accession Version	XM_017006638.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	semaphorin-5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC 
GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC 
ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG 
GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT 
GAGTGGTGTG AGCGTGAGAT ATCCAGCATC GCACCTGGAG AGCTGTGCTG TCTGCTCCTT 
TCCTTCCTGC CTCAGGAGGA GTGTCAGAAC TACGTGCGAG TCCTGATCGT CGCCGGCCGG 
AAGGTGTTCA TGTGTGGAAC CAATGCCTTT TCCCCCATGT GCACCAGCAG ACAGGTGGGG 
AACCTCAGCC GGACTATTGA GAAGATCAAT GGTGTGGCCC GCTGCCCCTA TGACCCACGC 
CACAACTCCA CAGCTGTCAT CTCCTCCCAG GGGGAGCTCT ATGCAGCCAC GGTCATCGAC 
TTCTCAGGTC GGGACCCTGC CATCTACCGC AGCCTGGGCA GTGGGCCACC GCTTCGCACT 
GCCCAATATA ACTCCAAGTG GCTTAATGAG CCAAACTTCG TGGCAGCCTA TGATATTGGG 
CTGTTTGCAT ACTTCTTCCT GCGGGAGAAC GCAGTGGAGC ACGACTGTGG ACGCACCGTG 
TACTCTCGCG TGGCCCGCGT GTGCAAGAAT GACGTGGGGG GCCGATTCCT GCTGGAGGAC 
ACATGGACCA CATTCATGAA GGCCCGGCTC AACTGCTCCC GCCCGGGCGA GGTCCCCTTC 
TACTATAACG AGCTGCAGAG TGCCTTCCAC TTGCCGGAGC AGGACCTCAT CTATGGAGTT 
TTCACAACCA ACGTAAACAG CATCGCGGCT TCTGCTGTCT GCGCCTTCAA CCTCAGTGCT 
ATCTCCCAGG CTTTCAATGG CCCATTTCGC TACCAGGAGA ACCCCAGGGC TGCCTGGCTC 
CCCATAGCCA ACCCCATCCC CAATTTCCAG TGTGGCACCC TGCCTGAGAC CGGTCCCAAC 
GAGAACCTGA CGGAGCGCAG CCTGCAGGAC GCGCAGCGCC TCTTCCTGAT GAGCGAGGCC 
GTGCAGCCGG TGACACCCGA GCCCTGTGTC ACCCAGGACA GCGTGCGCTT CTCACACCTC 
GTGGTGGACC TGGTGCAGGC TAAAGACACG CTCTACCATG TACTCTACAT TGGCACCGAG 
TCGGGCACCA TCCTGAAGGC GCTGTCCACG GCGAGCCGCA GCCTCCACGG CTGCTACCTG 
GAGGAGCTGC ACGTGCTGCC CCCCGGGCGC CGCGAGCCCC TGCGCAGCCT GCGCATCCTG 
CACAGCGCCC GCGCGCTCTT CGTGGGGCTG AGAGACGGCG TCCTGCGGGT CCCACTGGAG 
AGGTGCGCCG CCTACCGCAG CCAGGGGGCA TGCCTGGGGG CCCGGGACCC GTACTGTGGC 
TGGGACGGGA AGCAGCAACG TTGCAGCACA CTCGAGGACA GCTCCAACAT GAGCCTCTGG 
ACCCAGAACA TCACCGCCTG TCCTGTGCGG AATGTGACAC GGGATGGGGG CTTCGGCCCA 
TGGTCACCAT GGCAACCATG TGAGCACTTG GATGGGGACA ACTCAGGCTC TTGCCTGTGT 
CGAGCTCGAT CCTGTGATTC CCCTCGACCC CGCTGTGGGG GCCTTGACTG CCTGGGGCCA 
GCCATCCACA TCGCCAACTG CTCCAGGAAT GGGGCGTGGA CCCCGTGGTC ATCGTGGGCG 
CTGTGCAGCA CGTCCTGTGG CATCGGCTTC CAGGTCCGCC AGCGAAGTTG CAGCAACCCT 
GCTCCCCGCC ACGGGGGCCG CATCTGCGTG GGCAAGAGCC GGGAGGAACG GTTCTGTAAT 
GAGAACACGC CTTGCCCGGT GCCCATCTTC TGGGCTTCCT GGGGCTCCTG GAGCAAGTGC 
AGCAGCAACT GTGGAGGGGG CATGCAGTCG CGGCGTCGGG CCTGCGAGAA CGGCAACTCC 
TGCCTGGGCT GCGGCGTGGA GTTCAAGACG TGCAACCCCG AGGGCTGCCC CGAAGTGCGG 
CGCAACACCC CCTGGACGCC GTGGCTGCCC GTGAACGTGA CGCAGGGCGG GGCACGGCAG 
GAGCAGCGGT TCCGCTTCAC CTGCCGCGCG CCCCTTGCAG ACCCGCACGG CCTGCAGTTC 
GGCAGGAGAA GGACCGAGAC GAGGACCTGT CCCGCGGACG GCTCCGGCTC CTGCGACACC 
GACGCCCTGG TGGAGGTCCT CCTGCGCAGC GGGAGCACCT CCCCGCACAC GGTGAGCGGG 
GGCTGGGCCG CCTGGGGCCC GTGGTCGTCC TGCTCCCGGG ACTGCGAGCT GGGCTTCCGC 
GTCCGCAAGA GAACGTGCAC TAACCCGGAG CCCCGCAACG GGGGCCTGCC CTGCGTGGGC 
GATGCTGCCG AGTACCAGGA CTGCAACCCC CAGGCTTGCC CAGTTCGGGG TGCTTGGTCC 
TGCTGGACCT CATGGTCTCC ATGCTCAGCT TCCTGTGGTG GGGGTCACTA TCAACGCACC 
CGTTCCTGCA CCAGCCCCGC ACCCTCCCCA GGTGAGGACA TCTGTCTCGG GCTGCACACG 
GAGGAGGCAC TATGTGCCAC ACAGGCCTGC CCAGAAGGCT GGTCGCCCTG GTCTGAGTGG 
AGTAAGTGCA CTGACGACGG AGCCCAGAGC CGAAGCCGGC ACTGTGAGGA GCTCCTCCCA 
GGGTCCAGCG CCTGTGCTGG AAACAGCAGC CAGAGCCGCC CCTGCCCCTA CAGCGAGATT 
CCCGTCATCC TGCCAGCCTC CAGCATGGAG GAGGCCACCG ACTGTGCAGG GTTCAATCTC 
ATCCACTTGG TGGCCACGGG CATCTCCTGC TTCTTGGGCT CTGGGCTCCT GACCCTAGCA 
GTGTACCTGT CTTGCCAGCA CTGCCAGCGT CAGTCCCAGG AGTCCACACT GGTCCATCCT 
GCCACCCCCA ACCATTTGCA CTACAAGGGC GGAGGCACCC CGAAGAATGA AAAGTACACA 
CCCATGGAAT TCAAGACCCT GAACAAGAAT AACTTGATCC CTGATGACAG AGCCAACTTC 
TACCCATTGC AGCAGACCAA TGTGTACACG ACTACTTACT ACCCAAGCCC CCTGAACAAA 
CACAGCTTCC GGCCCGAGGC CTCACCTGGA CAACGGTGCT TCCCCAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862127.1
CDS	540..3893
Translation	MVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEWCEREISSIAPGELCCLLLSFLPQEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCNPEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGWAAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIHLVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	XM_017006638.1
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 5B (SEMA5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006638.1

ORF Insert Sequence:

ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC 
GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC 
ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG 
GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT 
GAGTGGTGTG AGCGTGAGAT ATCCAGCATC GCACCTGGAG AGCTGTGCTG TCTGCTCCTT 
TCCTTCCTGC CTCAGGAGGA GTGTCAGAAC TACGTGCGAG TCCTGATCGT CGCCGGCCGG 
AAGGTGTTCA TGTGTGGAAC CAATGCCTTT TCCCCCATGT GCACCAGCAG ACAGGTGGGG 
AACCTCAGCC GGACTATTGA GAAGATCAAT GGTGTGGCCC GCTGCCCCTA TGACCCACGC 
CACAACTCCA CAGCTGTCAT CTCCTCCCAG GGGGAGCTCT ATGCAGCCAC GGTCATCGAC 
TTCTCAGGTC GGGACCCTGC CATCTACCGC AGCCTGGGCA GTGGGCCACC GCTTCGCACT 
GCCCAATATA ACTCCAAGTG GCTTAATGAG CCAAACTTCG TGGCAGCCTA TGATATTGGG 
CTGTTTGCAT ACTTCTTCCT GCGGGAGAAC GCAGTGGAGC ACGACTGTGG ACGCACCGTG 
TACTCTCGCG TGGCCCGCGT GTGCAAGAAT GACGTGGGGG GCCGATTCCT GCTGGAGGAC 
ACATGGACCA CATTCATGAA GGCCCGGCTC AACTGCTCCC GCCCGGGCGA GGTCCCCTTC 
TACTATAACG AGCTGCAGAG TGCCTTCCAC TTGCCGGAGC AGGACCTCAT CTATGGAGTT 
TTCACAACCA ACGTAAACAG CATCGCGGCT TCTGCTGTCT GCGCCTTCAA CCTCAGTGCT 
ATCTCCCAGG CTTTCAATGG CCCATTTCGC TACCAGGAGA ACCCCAGGGC TGCCTGGCTC 
CCCATAGCCA ACCCCATCCC CAATTTCCAG TGTGGCACCC TGCCTGAGAC CGGTCCCAAC 
GAGAACCTGA CGGAGCGCAG CCTGCAGGAC GCGCAGCGCC TCTTCCTGAT GAGCGAGGCC 
GTGCAGCCGG TGACACCCGA GCCCTGTGTC ACCCAGGACA GCGTGCGCTT CTCACACCTC 
GTGGTGGACC TGGTGCAGGC TAAAGACACG CTCTACCATG TACTCTACAT TGGCACCGAG 
TCGGGCACCA TCCTGAAGGC GCTGTCCACG GCGAGCCGCA GCCTCCACGG CTGCTACCTG 
GAGGAGCTGC ACGTGCTGCC CCCCGGGCGC CGCGAGCCCC TGCGCAGCCT GCGCATCCTG 
CACAGCGCCC GCGCGCTCTT CGTGGGGCTG AGAGACGGCG TCCTGCGGGT CCCACTGGAG 
AGGTGCGCCG CCTACCGCAG CCAGGGGGCA TGCCTGGGGG CCCGGGACCC GTACTGTGGC 
TGGGACGGGA AGCAGCAACG TTGCAGCACA CTCGAGGACA GCTCCAACAT GAGCCTCTGG 
ACCCAGAACA TCACCGCCTG TCCTGTGCGG AATGTGACAC GGGATGGGGG CTTCGGCCCA 
TGGTCACCAT GGCAACCATG TGAGCACTTG GATGGGGACA ACTCAGGCTC TTGCCTGTGT 
CGAGCTCGAT CCTGTGATTC CCCTCGACCC CGCTGTGGGG GCCTTGACTG CCTGGGGCCA 
GCCATCCACA TCGCCAACTG CTCCAGGAAT GGGGCGTGGA CCCCGTGGTC ATCGTGGGCG 
CTGTGCAGCA CGTCCTGTGG CATCGGCTTC CAGGTCCGCC AGCGAAGTTG CAGCAACCCT 
GCTCCCCGCC ACGGGGGCCG CATCTGCGTG GGCAAGAGCC GGGAGGAACG GTTCTGTAAT 
GAGAACACGC CTTGCCCGGT GCCCATCTTC TGGGCTTCCT GGGGCTCCTG GAGCAAGTGC 
AGCAGCAACT GTGGAGGGGG CATGCAGTCG CGGCGTCGGG CCTGCGAGAA CGGCAACTCC 
TGCCTGGGCT GCGGCGTGGA GTTCAAGACG TGCAACCCCG AGGGCTGCCC CGAAGTGCGG 
CGCAACACCC CCTGGACGCC GTGGCTGCCC GTGAACGTGA CGCAGGGCGG GGCACGGCAG 
GAGCAGCGGT TCCGCTTCAC CTGCCGCGCG CCCCTTGCAG ACCCGCACGG CCTGCAGTTC 
GGCAGGAGAA GGACCGAGAC GAGGACCTGT CCCGCGGACG GCTCCGGCTC CTGCGACACC 
GACGCCCTGG TGGAGGTCCT CCTGCGCAGC GGGAGCACCT CCCCGCACAC GGTGAGCGGG 
GGCTGGGCCG CCTGGGGCCC GTGGTCGTCC TGCTCCCGGG ACTGCGAGCT GGGCTTCCGC 
GTCCGCAAGA GAACGTGCAC TAACCCGGAG CCCCGCAACG GGGGCCTGCC CTGCGTGGGC 
GATGCTGCCG AGTACCAGGA CTGCAACCCC CAGGCTTGCC CAGTTCGGGG TGCTTGGTCC 
TGCTGGACCT CATGGTCTCC ATGCTCAGCT TCCTGTGGTG GGGGTCACTA TCAACGCACC 
CGTTCCTGCA CCAGCCCCGC ACCCTCCCCA GGTGAGGACA TCTGTCTCGG GCTGCACACG 
GAGGAGGCAC TATGTGCCAC ACAGGCCTGC CCAGAAGGCT GGTCGCCCTG GTCTGAGTGG 
AGTAAGTGCA CTGACGACGG AGCCCAGAGC CGAAGCCGGC ACTGTGAGGA GCTCCTCCCA 
GGGTCCAGCG CCTGTGCTGG AAACAGCAGC CAGAGCCGCC CCTGCCCCTA CAGCGAGATT 
CCCGTCATCC TGCCAGCCTC CAGCATGGAG GAGGCCACCG ACTGTGCAGG GTTCAATCTC 
ATCCACTTGG TGGCCACGGG CATCTCCTGC TTCTTGGGCT CTGGGCTCCT GACCCTAGCA 
GTGTACCTGT CTTGCCAGCA CTGCCAGCGT CAGTCCCAGG AGTCCACACT GGTCCATCCT 
GCCACCCCCA ACCATTTGCA CTACAAGGGC GGAGGCACCC CGAAGAATGA AAAGTACACA 
CCCATGGAAT TCAAGACCCT GAACAAGAAT AACTTGATCC CTGATGACAG AGCCAACTTC 
TACCCATTGC AGCAGACCAA TGTGTACACG ACTACTTACT ACCCAAGCCC CCTGAACAAA 
CACAGCTTCC GGCCCGAGGC CTCACCTGGA CAACGGTGCT TCCCCAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5B ( NM_001256348.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243277.1 Homo sapiens semaphorin 5B (SEMA5B), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu00906
Clone ID Related Accession (Same CDS sequence)	NM_001256348.1
Accession Version	NM_001256348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	semaphorin-5B isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC083797.16, AK294528.1 and AC078794.13. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region, uses a downstream start codon and uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The resulting protein (isoform 3) is shorter and has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK294528.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END##

ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC 
GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC 
ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG 
GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT 
GAGGAGGAGT GTCAGAACTA CGTGCGAGTC CTGATCGTCG CCGGCCGGAA GGTGTTCATG 
TGTGGAACCA ATGCCTTTTC CCCCATGTGC ACCAGCAGAC AGGTGGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAATGG TGTGGCCCGC TGCCCCTATG ACCCACGCCA CAACTCCACA 
GCTGTCATCT CCTCCCAGGG GGAGCTCTAT GCAGCCACGG TCATCGACTT CTCAGGTCGG 
GACCCTGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAATATAAC 
TCCAAGTGGC TTAATGAGCC AAACTTCGTG GCAGCCTATG ATATTGGGCT GTTTGCATAC 
TTCTTCCTGC GGGAGAACGC AGTGGAGCAC GACTGTGGAC GCACCGTGTA CTCTCGCGTG 
GCCCGCGTGT GCAAGAATGA CGTGGGGGGC CGATTCCTGC TGGAGGACAC ATGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGCGAGG TCCCCTTCTA CTATAACGAG 
CTGCAGAGTG CCTTCCACTT GCCGGAGCAG GACCTCATCT ATGGAGTTTT CACAACCAAC 
GTAAACAGCA TCGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCTAT CTCCCAGGCT 
TTCAATGGCC CATTTCGCTA CCAGGAGAAC CCCAGGGCTG CCTGGCTCCC CATAGCCAAC 
CCCATCCCCA ATTTCCAGTG TGGCACCCTG CCTGAGACCG GTCCCAACGA GAACCTGACG 
GAGCGCAGCC TGCAGGACGC GCAGCGCCTC TTCCTGATGA GCGAGGCCGT GCAGCCGGTG 
ACACCCGAGC CCTGTGTCAC CCAGGACAGC GTGCGCTTCT CACACCTCGT GGTGGACCTG 
GTGCAGGCTA AAGACACGCT CTACCATGTA CTCTACATTG GCACCGAGTC GGGCACCATC 
CTGAAGGCGC TGTCCACGGC GAGCCGCAGC CTCCACGGCT GCTACCTGGA GGAGCTGCAC 
GTGCTGCCCC CCGGGCGCCG CGAGCCCCTG CGCAGCCTGC GCATCCTGCA CAGCGCCCGC 
GCGCTCTTCG TGGGGCTGAG AGACGGCGTC CTGCGGGTCC CACTGGAGAG GTGCGCCGCC 
TACCGCAGCC AGGGGGCATG CCTGGGGGCC CGGGACCCGT ACTGTGGCTG GGACGGGAAG 
CAGCAACGTT GCAGCACACT CGAGGACAGC TCCAACATGA GCCTCTGGAC CCAGAACATC 
ACCGCCTGTC CTGTGCGGAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
CAACCATGTG AGCACTTGGA TGGGGACAAC TCAGGCTCTT GCCTGTGTCG AGCTCGATCC 
TGTGATTCCC CTCGACCCCG CTGTGGGGGC CTTGACTGCC TGGGGCCAGC CATCCACATC 
GCCAACTGCT CCAGGAATGG GGCGTGGACC CCGTGGTCAT CGTGGGCGCT GTGCAGCACG 
TCCTGTGGCA TCGGCTTCCA GGTCCGCCAG CGAAGTTGCA GCAACCCTGC TCCCCGCCAC 
GGGGGCCGCA TCTGCGTGGG CAAGAGCCGG GAGGAACGGT TCTGTAATGA GAACACGCCT 
TGCCCGGTGC CCATCTTCTG GGCTTCCTGG GGCTCCTGGA GCAAGTGCAG CAGCAACTGT 
GGAGGGGGCA TGCAGTCGCG GCGTCGGGCC TGCGAGAACG GCAACTCCTG CCTGGGCTGC 
GGCGTGGAGT TCAAGACGTG CAACCCCGAG GGCTGCCCCG AAGTGCGGCG CAACACCCCC 
TGGACGCCGT GGCTGCCCGT GAACGTGACG CAGGGCGGGG CACGGCAGGA GCAGCGGTTC 
CGCTTCACCT GCCGCGCGCC CCTTGCAGAC CCGCACGGCC TGCAGTTCGG CAGGAGAAGG 
ACCGAGACGA GGACCTGTCC CGCGGACGGC TCCGGCTCCT GCGACACCGA CGCCCTGGTG 
GAGGTCCTCC TGCGCAGCGG GAGCACCTCC CCGCACACGG TGAGCGGGGG CTGGGCCGCC 
TGGGGCCCGT GGTCGTCCTG CTCCCGGGAC TGCGAGCTGG GCTTCCGCGT CCGCAAGAGA 
ACGTGCACTA ACCCGGAGCC CCGCAACGGG GGCCTGCCCT GCGTGGGCGA TGCTGCCGAG 
TACCAGGACT GCAACCCCCA GGCTTGCCCA GGTGAGGACA TCTGTCTCGG GCTGCACACG 
GAGGAGGCAC TATGTGCCAC ACAGGCCTGC CCAGAAGGCT GGTCGCCCTG GTCTGAGTGG 
AGTAAGTGCA CTGACGACGG AGCCCAGAGC CGAAGCCGGC ACTGTGAGGA GCTCCTCCCA 
GGGTCCAGCG CCTGTGCTGG AAACAGCAGC CAGAGCCGCC CCTGCCCCTA CAGCGAGATT 
CCCGTCATCC TGCCAGCCTC CAGCATGGAG GAGGCCACCG ACTGTGCAGG GTTCAATCTC 
ATCCACTTGG TGGCCACGGG CATCTCCTGC TTCTTGGGCT CTGGGCTCCT GACCCTAGCA 
GTGTACCTGT CTTGCCAGCA CTGCCAGCGT CAGTCCCAGG AGTCCACACT GGTCCATCCT 
GCCACCCCCA ACCATTTGCA CTACAAGGGC GGAGGCACCC CGAAGAATGA AAAGTACACA 
CCCATGGAAT TCAAGACCCT GAACAAGAAT AACTTGATCC CTGATGACAG AGCCAACTTC 
TACCCATTGC AGCAGACCAA TGTGTACACG ACTACTTACT ACCCAAGCCC CCTGAACAAA 
CACAGCTTCC GGCCCGAGGC CTCACCTGGA CAACGGTGCT TCCCCAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243277.1
CDS	400..3573
Translation	MVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCNPEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGWAAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPGEDICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIHLVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	NM_001256348.1
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 5B (SEMA5B), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256348.1

ORF Insert Sequence:

ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC 
GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC 
ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC 
ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG 
GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT 
GAGGAGGAGT GTCAGAACTA CGTGCGAGTC CTGATCGTCG CCGGCCGGAA GGTGTTCATG 
TGTGGAACCA ATGCCTTTTC CCCCATGTGC ACCAGCAGAC AGGTGGGGAA CCTCAGCCGG 
ACTATTGAGA AGATCAATGG TGTGGCCCGC TGCCCCTATG ACCCACGCCA CAACTCCACA 
GCTGTCATCT CCTCCCAGGG GGAGCTCTAT GCAGCCACGG TCATCGACTT CTCAGGTCGG 
GACCCTGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAATATAAC 
TCCAAGTGGC TTAATGAGCC AAACTTCGTG GCAGCCTATG ATATTGGGCT GTTTGCATAC 
TTCTTCCTGC GGGAGAACGC AGTGGAGCAC GACTGTGGAC GCACCGTGTA CTCTCGCGTG 
GCCCGCGTGT GCAAGAATGA CGTGGGGGGC CGATTCCTGC TGGAGGACAC ATGGACCACA 
TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGCGAGG TCCCCTTCTA CTATAACGAG 
CTGCAGAGTG CCTTCCACTT GCCGGAGCAG GACCTCATCT ATGGAGTTTT CACAACCAAC 
GTAAACAGCA TCGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCTAT CTCCCAGGCT 
TTCAATGGCC CATTTCGCTA CCAGGAGAAC CCCAGGGCTG CCTGGCTCCC CATAGCCAAC 
CCCATCCCCA ATTTCCAGTG TGGCACCCTG CCTGAGACCG GTCCCAACGA GAACCTGACG 
GAGCGCAGCC TGCAGGACGC GCAGCGCCTC TTCCTGATGA GCGAGGCCGT GCAGCCGGTG 
ACACCCGAGC CCTGTGTCAC CCAGGACAGC GTGCGCTTCT CACACCTCGT GGTGGACCTG 
GTGCAGGCTA AAGACACGCT CTACCATGTA CTCTACATTG GCACCGAGTC GGGCACCATC 
CTGAAGGCGC TGTCCACGGC GAGCCGCAGC CTCCACGGCT GCTACCTGGA GGAGCTGCAC 
GTGCTGCCCC CCGGGCGCCG CGAGCCCCTG CGCAGCCTGC GCATCCTGCA CAGCGCCCGC 
GCGCTCTTCG TGGGGCTGAG AGACGGCGTC CTGCGGGTCC CACTGGAGAG GTGCGCCGCC 
TACCGCAGCC AGGGGGCATG CCTGGGGGCC CGGGACCCGT ACTGTGGCTG GGACGGGAAG 
CAGCAACGTT GCAGCACACT CGAGGACAGC TCCAACATGA GCCTCTGGAC CCAGAACATC 
ACCGCCTGTC CTGTGCGGAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG 
CAACCATGTG AGCACTTGGA TGGGGACAAC TCAGGCTCTT GCCTGTGTCG AGCTCGATCC 
TGTGATTCCC CTCGACCCCG CTGTGGGGGC CTTGACTGCC TGGGGCCAGC CATCCACATC 
GCCAACTGCT CCAGGAATGG GGCGTGGACC CCGTGGTCAT CGTGGGCGCT GTGCAGCACG 
TCCTGTGGCA TCGGCTTCCA GGTCCGCCAG CGAAGTTGCA GCAACCCTGC TCCCCGCCAC 
GGGGGCCGCA TCTGCGTGGG CAAGAGCCGG GAGGAACGGT TCTGTAATGA GAACACGCCT 
TGCCCGGTGC CCATCTTCTG GGCTTCCTGG GGCTCCTGGA GCAAGTGCAG CAGCAACTGT 
GGAGGGGGCA TGCAGTCGCG GCGTCGGGCC TGCGAGAACG GCAACTCCTG CCTGGGCTGC 
GGCGTGGAGT TCAAGACGTG CAACCCCGAG GGCTGCCCCG AAGTGCGGCG CAACACCCCC 
TGGACGCCGT GGCTGCCCGT GAACGTGACG CAGGGCGGGG CACGGCAGGA GCAGCGGTTC 
CGCTTCACCT GCCGCGCGCC CCTTGCAGAC CCGCACGGCC TGCAGTTCGG CAGGAGAAGG 
ACCGAGACGA GGACCTGTCC CGCGGACGGC TCCGGCTCCT GCGACACCGA CGCCCTGGTG 
GAGGTCCTCC TGCGCAGCGG GAGCACCTCC CCGCACACGG TGAGCGGGGG CTGGGCCGCC 
TGGGGCCCGT GGTCGTCCTG CTCCCGGGAC TGCGAGCTGG GCTTCCGCGT CCGCAAGAGA 
ACGTGCACTA ACCCGGAGCC CCGCAACGGG GGCCTGCCCT GCGTGGGCGA TGCTGCCGAG 
TACCAGGACT GCAACCCCCA GGCTTGCCCA GGTGAGGACA TCTGTCTCGG GCTGCACACG 
GAGGAGGCAC TATGTGCCAC ACAGGCCTGC CCAGAAGGCT GGTCGCCCTG GTCTGAGTGG 
AGTAAGTGCA CTGACGACGG AGCCCAGAGC CGAAGCCGGC ACTGTGAGGA GCTCCTCCCA 
GGGTCCAGCG CCTGTGCTGG AAACAGCAGC CAGAGCCGCC CCTGCCCCTA CAGCGAGATT 
CCCGTCATCC TGCCAGCCTC CAGCATGGAG GAGGCCACCG ACTGTGCAGG GTTCAATCTC 
ATCCACTTGG TGGCCACGGG CATCTCCTGC TTCTTGGGCT CTGGGCTCCT GACCCTAGCA 
GTGTACCTGT CTTGCCAGCA CTGCCAGCGT CAGTCCCAGG AGTCCACACT GGTCCATCCT 
GCCACCCCCA ACCATTTGCA CTACAAGGGC GGAGGCACCC CGAAGAATGA AAAGTACACA 
CCCATGGAAT TCAAGACCCT GAACAAGAAT AACTTGATCC CTGATGACAG AGCCAACTTC 
TACCCATTGC AGCAGACCAA TGTGTACACG ACTACTTACT ACCCAAGCCC CCTGAACAAA 
CACAGCTTCC GGCCCGAGGC CTCACCTGGA CAACGGTGCT TCCCCAACAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5B ( NM_001031702.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001026872.2 Homo sapiens semaphorin 5B (SEMA5B), transcript variant 1, mRNA.

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CloneID	OHu01368
Clone ID Related Accession (Same CDS sequence)	NM_001031702.4 , NM_001031702.3 , NM_001256346.1
Accession Version	NM_001031702.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	semaphorin-5B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA115188.1, DA797213.1, AB040878.1, BC077726.1, AK091481.1, BC002776.2 and AC078794.13. On May 17, 2019 this sequence version replaced NM_001031702.3. Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 2 encode the same protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AB040878.1, BC077726.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000357599.8/ ENSP00000350215.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGCCCTGTG GCTTCAGTCC GTCTCCTGTT GCCCACCACC TCGTCCCTGG GCCGCCTGAT 
ACCCCAGCCC AACAGCTAAG GTGTGGATGG ACAGTAGGGG GCTGGCTTCT CTCACTGGTC 
AGGGGTCTTC TCCCCTGTCT GCCTCCCGGA GCTAGGACTG CAGAGGGGCC TATCATGGTG 
CTTGCAGGCC CCCTGGCTGT CTCGCTGTTG CTGCCCAGCC TCACACTGCT GGTGTCCCAC 
CTCTCCAGCT CCCAGGATGT CTCCAGTGAG CCCAGCAGTG AGCAGCAGCT GTGCGCCCTT 
AGCAAGCACC CCACCGTGGC CTTTGAAGAC CTGCAGCCGT GGGTCTCTAA CTTCACCTAC 
CCTGGAGCCC GGGATTTCTC CCAGCTGGCT TTGGACCCCT CCGGGAACCA GCTCATCGTG 
GGAGCCAGGA ACTACCTCTT CAGACTCAGC CTTGCCAATG TCTCTCTTCT TCAGGCCACA 
GAGTGGGCCT CCAGTGAGGA CACGCGCCGC TCCTGCCAAA GCAAAGGGAA GACTGAGGAG 
GAGTGTCAGA ACTACGTGCG AGTCCTGATC GTCGCCGGCC GGAAGGTGTT CATGTGTGGA 
ACCAATGCCT TTTCCCCCAT GTGCACCAGC AGACAGGTGG GGAACCTCAG CCGGACTATT 
GAGAAGATCA ATGGTGTGGC CCGCTGCCCC TATGACCCAC GCCACAACTC CACAGCTGTC 
ATCTCCTCCC AGGGGGAGCT CTATGCAGCC ACGGTCATCG ACTTCTCAGG TCGGGACCCT 
GCCATCTACC GCAGCCTGGG CAGTGGGCCA CCGCTTCGCA CTGCCCAATA TAACTCCAAG 
TGGCTTAATG AGCCAAACTT CGTGGCAGCC TATGATATTG GGCTGTTTGC ATACTTCTTC 
CTGCGGGAGA ACGCAGTGGA GCACGACTGT GGACGCACCG TGTACTCTCG CGTGGCCCGC 
GTGTGCAAGA ATGACGTGGG GGGCCGATTC CTGCTGGAGG ACACATGGAC CACATTCATG 
AAGGCCCGGC TCAACTGCTC CCGCCCGGGC GAGGTCCCCT TCTACTATAA CGAGCTGCAG 
AGTGCCTTCC ACTTGCCGGA GCAGGACCTC ATCTATGGAG TTTTCACAAC CAACGTAAAC 
AGCATCGCGG CTTCTGCTGT CTGCGCCTTC AACCTCAGTG CTATCTCCCA GGCTTTCAAT 
GGCCCATTTC GCTACCAGGA GAACCCCAGG GCTGCCTGGC TCCCCATAGC CAACCCCATC 
CCCAATTTCC AGTGTGGCAC CCTGCCTGAG ACCGGTCCCA ACGAGAACCT GACGGAGCGC 
AGCCTGCAGG ACGCGCAGCG CCTCTTCCTG ATGAGCGAGG CCGTGCAGCC GGTGACACCC 
GAGCCCTGTG TCACCCAGGA CAGCGTGCGC TTCTCACACC TCGTGGTGGA CCTGGTGCAG 
GCTAAAGACA CGCTCTACCA TGTACTCTAC ATTGGCACCG AGTCGGGCAC CATCCTGAAG 
GCGCTGTCCA CGGCGAGCCG CAGCCTCCAC GGCTGCTACC TGGAGGAGCT GCACGTGCTG 
CCCCCCGGGC GCCGCGAGCC CCTGCGCAGC CTGCGCATCC TGCACAGCGC CCGCGCGCTC 
TTCGTGGGGC TGAGAGACGG CGTCCTGCGG GTCCCACTGG AGAGGTGCGC CGCCTACCGC 
AGCCAGGGGG CATGCCTGGG GGCCCGGGAC CCGTACTGTG GCTGGGACGG GAAGCAGCAA 
CGTTGCAGCA CACTCGAGGA CAGCTCCAAC ATGAGCCTCT GGACCCAGAA CATCACCGCC 
TGTCCTGTGC GGAATGTGAC ACGGGATGGG GGCTTCGGCC CATGGTCACC ATGGCAACCA 
TGTGAGCACT TGGATGGGGA CAACTCAGGC TCTTGCCTGT GTCGAGCTCG ATCCTGTGAT 
TCCCCTCGAC CCCGCTGTGG GGGCCTTGAC TGCCTGGGGC CAGCCATCCA CATCGCCAAC 
TGCTCCAGGA ATGGGGCGTG GACCCCGTGG TCATCGTGGG CGCTGTGCAG CACGTCCTGT 
GGCATCGGCT TCCAGGTCCG CCAGCGAAGT TGCAGCAACC CTGCTCCCCG CCACGGGGGC 
CGCATCTGCG TGGGCAAGAG CCGGGAGGAA CGGTTCTGTA ATGAGAACAC GCCTTGCCCG 
GTGCCCATCT TCTGGGCTTC CTGGGGCTCC TGGAGCAAGT GCAGCAGCAA CTGTGGAGGG 
GGCATGCAGT CGCGGCGTCG GGCCTGCGAG AACGGCAACT CCTGCCTGGG CTGCGGCGTG 
GAGTTCAAGA CGTGCAACCC CGAGGGCTGC CCCGAAGTGC GGCGCAACAC CCCCTGGACG 
CCGTGGCTGC CCGTGAACGT GACGCAGGGC GGGGCACGGC AGGAGCAGCG GTTCCGCTTC 
ACCTGCCGCG CGCCCCTTGC AGACCCGCAC GGCCTGCAGT TCGGCAGGAG AAGGACCGAG 
ACGAGGACCT GTCCCGCGGA CGGCTCCGGC TCCTGCGACA CCGACGCCCT GGTGGAGGTC 
CTCCTGCGCA GCGGGAGCAC CTCCCCGCAC ACGGTGAGCG GGGGCTGGGC CGCCTGGGGC 
CCGTGGTCGT CCTGCTCCCG GGACTGCGAG CTGGGCTTCC GCGTCCGCAA GAGAACGTGC 
ACTAACCCGG AGCCCCGCAA CGGGGGCCTG CCCTGCGTGG GCGATGCTGC CGAGTACCAG 
GACTGCAACC CCCAGGCTTG CCCAGTTCGG GGTGCTTGGT CCTGCTGGAC CTCATGGTCT 
CCATGCTCAG CTTCCTGTGG TGGGGGTCAC TATCAACGCA CCCGTTCCTG CACCAGCCCC 
GCACCCTCCC CAGGTGAGGA CATCTGTCTC GGGCTGCACA CGGAGGAGGC ACTATGTGCC 
ACACAGGCCT GCCCAGAAGG CTGGTCGCCC TGGTCTGAGT GGAGTAAGTG CACTGACGAC 
GGAGCCCAGA GCCGAAGCCG GCACTGTGAG GAGCTCCTCC CAGGGTCCAG CGCCTGTGCT 
GGAAACAGCA GCCAGAGCCG CCCCTGCCCC TACAGCGAGA TTCCCGTCAT CCTGCCAGCC 
TCCAGCATGG AGGAGGCCAC CGACTGTGCA GGGTTCAATC TCATCCACTT GGTGGCCACG 
GGCATCTCCT GCTTCTTGGG CTCTGGGCTC CTGACCCTAG CAGTGTACCT GTCTTGCCAG 
CACTGCCAGC GTCAGTCCCA GGAGTCCACA CTGGTCCATC CTGCCACCCC CAACCATTTG 
CACTACAAGG GCGGAGGCAC CCCGAAGAAT GAAAAGTACA CACCCATGGA ATTCAAGACC 
CTGAACAAGA ATAACTTGAT CCCTGATGAC AGAGCCAACT TCTACCCATT GCAGCAGACC 
AATGTGTACA CGACTACTTA CTACCCAAGC CCCCTGAACA AACACAGCTT CCGGCCCGAG 
GCCTCACCTG GACAACGGTG CTTCCCCAAC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026872.2
CDS	339..3794
Translation	MPCGFSPSPVAHHLVPGPPDTPAQQLRCGWTVGGWLLSLVRGLLPCLPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCNPEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGWAAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIHLVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	NM_001031702.4
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 5B (SEMA5B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031702.4

ORF Insert Sequence:

ATGCCCTGTG GCTTCAGTCC GTCTCCTGTT GCCCACCACC TCGTCCCTGG GCCGCCTGAT 
ACCCCAGCCC AACAGCTAAG GTGTGGATGG ACAGTAGGGG GCTGGCTTCT CTCACTGGTC 
AGGGGTCTTC TCCCCTGTCT GCCTCCCGGA GCTAGGACTG CAGAGGGGCC TATCATGGTG 
CTTGCAGGCC CCCTGGCTGT CTCGCTGTTG CTGCCCAGCC TCACACTGCT GGTGTCCCAC 
CTCTCCAGCT CCCAGGATGT CTCCAGTGAG CCCAGCAGTG AGCAGCAGCT GTGCGCCCTT 
AGCAAGCACC CCACCGTGGC CTTTGAAGAC CTGCAGCCGT GGGTCTCTAA CTTCACCTAC 
CCTGGAGCCC GGGATTTCTC CCAGCTGGCT TTGGACCCCT CCGGGAACCA GCTCATCGTG 
GGAGCCAGGA ACTACCTCTT CAGACTCAGC CTTGCCAATG TCTCTCTTCT TCAGGCCACA 
GAGTGGGCCT CCAGTGAGGA CACGCGCCGC TCCTGCCAAA GCAAAGGGAA GACTGAGGAG 
GAGTGTCAGA ACTACGTGCG AGTCCTGATC GTCGCCGGCC GGAAGGTGTT CATGTGTGGA 
ACCAATGCCT TTTCCCCCAT GTGCACCAGC AGACAGGTGG GGAACCTCAG CCGGACTATT 
GAGAAGATCA ATGGTGTGGC CCGCTGCCCC TATGACCCAC GCCACAACTC CACAGCTGTC 
ATCTCCTCCC AGGGGGAGCT CTATGCAGCC ACGGTCATCG ACTTCTCAGG TCGGGACCCT 
GCCATCTACC GCAGCCTGGG CAGTGGGCCA CCGCTTCGCA CTGCCCAATA TAACTCCAAG 
TGGCTTAATG AGCCAAACTT CGTGGCAGCC TATGATATTG GGCTGTTTGC ATACTTCTTC 
CTGCGGGAGA ACGCAGTGGA GCACGACTGT GGACGCACCG TGTACTCTCG CGTGGCCCGC 
GTGTGCAAGA ATGACGTGGG GGGCCGATTC CTGCTGGAGG ACACATGGAC CACATTCATG 
AAGGCCCGGC TCAACTGCTC CCGCCCGGGC GAGGTCCCCT TCTACTATAA CGAGCTGCAG 
AGTGCCTTCC ACTTGCCGGA GCAGGACCTC ATCTATGGAG TTTTCACAAC CAACGTAAAC 
AGCATCGCGG CTTCTGCTGT CTGCGCCTTC AACCTCAGTG CTATCTCCCA GGCTTTCAAT 
GGCCCATTTC GCTACCAGGA GAACCCCAGG GCTGCCTGGC TCCCCATAGC CAACCCCATC 
CCCAATTTCC AGTGTGGCAC CCTGCCTGAG ACCGGTCCCA ACGAGAACCT GACGGAGCGC 
AGCCTGCAGG ACGCGCAGCG CCTCTTCCTG ATGAGCGAGG CCGTGCAGCC GGTGACACCC 
GAGCCCTGTG TCACCCAGGA CAGCGTGCGC TTCTCACACC TCGTGGTGGA CCTGGTGCAG 
GCTAAAGACA CGCTCTACCA TGTACTCTAC ATTGGCACCG AGTCGGGCAC CATCCTGAAG 
GCGCTGTCCA CGGCGAGCCG CAGCCTCCAC GGCTGCTACC TGGAGGAGCT GCACGTGCTG 
CCCCCCGGGC GCCGCGAGCC CCTGCGCAGC CTGCGCATCC TGCACAGCGC CCGCGCGCTC 
TTCGTGGGGC TGAGAGACGG CGTCCTGCGG GTCCCACTGG AGAGGTGCGC CGCCTACCGC 
AGCCAGGGGG CATGCCTGGG GGCCCGGGAC CCGTACTGTG GCTGGGACGG GAAGCAGCAA 
CGTTGCAGCA CACTCGAGGA CAGCTCCAAC ATGAGCCTCT GGACCCAGAA CATCACCGCC 
TGTCCTGTGC GGAATGTGAC ACGGGATGGG GGCTTCGGCC CATGGTCACC ATGGCAACCA 
TGTGAGCACT TGGATGGGGA CAACTCAGGC TCTTGCCTGT GTCGAGCTCG ATCCTGTGAT 
TCCCCTCGAC CCCGCTGTGG GGGCCTTGAC TGCCTGGGGC CAGCCATCCA CATCGCCAAC 
TGCTCCAGGA ATGGGGCGTG GACCCCGTGG TCATCGTGGG CGCTGTGCAG CACGTCCTGT 
GGCATCGGCT TCCAGGTCCG CCAGCGAAGT TGCAGCAACC CTGCTCCCCG CCACGGGGGC 
CGCATCTGCG TGGGCAAGAG CCGGGAGGAA CGGTTCTGTA ATGAGAACAC GCCTTGCCCG 
GTGCCCATCT TCTGGGCTTC CTGGGGCTCC TGGAGCAAGT GCAGCAGCAA CTGTGGAGGG 
GGCATGCAGT CGCGGCGTCG GGCCTGCGAG AACGGCAACT CCTGCCTGGG CTGCGGCGTG 
GAGTTCAAGA CGTGCAACCC CGAGGGCTGC CCCGAAGTGC GGCGCAACAC CCCCTGGACG 
CCGTGGCTGC CCGTGAACGT GACGCAGGGC GGGGCACGGC AGGAGCAGCG GTTCCGCTTC 
ACCTGCCGCG CGCCCCTTGC AGACCCGCAC GGCCTGCAGT TCGGCAGGAG AAGGACCGAG 
ACGAGGACCT GTCCCGCGGA CGGCTCCGGC TCCTGCGACA CCGACGCCCT GGTGGAGGTC 
CTCCTGCGCA GCGGGAGCAC CTCCCCGCAC ACGGTGAGCG GGGGCTGGGC CGCCTGGGGC 
CCGTGGTCGT CCTGCTCCCG GGACTGCGAG CTGGGCTTCC GCGTCCGCAA GAGAACGTGC 
ACTAACCCGG AGCCCCGCAA CGGGGGCCTG CCCTGCGTGG GCGATGCTGC CGAGTACCAG 
GACTGCAACC CCCAGGCTTG CCCAGTTCGG GGTGCTTGGT CCTGCTGGAC CTCATGGTCT 
CCATGCTCAG CTTCCTGTGG TGGGGGTCAC TATCAACGCA CCCGTTCCTG CACCAGCCCC 
GCACCCTCCC CAGGTGAGGA CATCTGTCTC GGGCTGCACA CGGAGGAGGC ACTATGTGCC 
ACACAGGCCT GCCCAGAAGG CTGGTCGCCC TGGTCTGAGT GGAGTAAGTG CACTGACGAC 
GGAGCCCAGA GCCGAAGCCG GCACTGTGAG GAGCTCCTCC CAGGGTCCAG CGCCTGTGCT 
GGAAACAGCA GCCAGAGCCG CCCCTGCCCC TACAGCGAGA TTCCCGTCAT CCTGCCAGCC 
TCCAGCATGG AGGAGGCCAC CGACTGTGCA GGGTTCAATC TCATCCACTT GGTGGCCACG 
GGCATCTCCT GCTTCTTGGG CTCTGGGCTC CTGACCCTAG CAGTGTACCT GTCTTGCCAG 
CACTGCCAGC GTCAGTCCCA GGAGTCCACA CTGGTCCATC CTGCCACCCC CAACCATTTG 
CACTACAAGG GCGGAGGCAC CCCGAAGAAT GAAAAGTACA CACCCATGGA ATTCAAGACC 
CTGAACAAGA ATAACTTGAT CCCTGATGAC AGAGCCAACT TCTACCCATT GCAGCAGACC 
AATGTGTACA CGACTACTTA CTACCCAAGC CCCCTGAACA AACACAGCTT CCGGCCCGAG 
GCCTCACCTG GACAACGGTG CTTCCCCAAC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5B ( NM_001031702.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001026872.2 Homo sapiens semaphorin 5B (SEMA5B), transcript variant 1, mRNA.

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CloneID	OHu01368
Clone ID Related Accession (Same CDS sequence)	NM_001031702.4 , NM_001031702.3 , NM_001256346.1
Accession Version	NM_001031702.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	semaphorin-5B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA115188.1, DA797213.1, AB040878.1, BC077726.1, AK091481.1, BC002776.2 and AC078794.13. On Jan 21, 2012 this sequence version replaced NM_001031702.2. Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 2 encode the same protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AB040878.1, BC077726.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCCCTGTG GCTTCAGTCC GTCTCCTGTT GCCCACCACC TCGTCCCTGG GCCGCCTGAT 
ACCCCAGCCC AACAGCTAAG GTGTGGATGG ACAGTAGGGG GCTGGCTTCT CTCACTGGTC 
AGGGGTCTTC TCCCCTGTCT GCCTCCCGGA GCTAGGACTG CAGAGGGGCC TATCATGGTG 
CTTGCAGGCC CCCTGGCTGT CTCGCTGTTG CTGCCCAGCC TCACACTGCT GGTGTCCCAC 
CTCTCCAGCT CCCAGGATGT CTCCAGTGAG CCCAGCAGTG AGCAGCAGCT GTGCGCCCTT 
AGCAAGCACC CCACCGTGGC CTTTGAAGAC CTGCAGCCGT GGGTCTCTAA CTTCACCTAC 
CCTGGAGCCC GGGATTTCTC CCAGCTGGCT TTGGACCCCT CCGGGAACCA GCTCATCGTG 
GGAGCCAGGA ACTACCTCTT CAGACTCAGC CTTGCCAATG TCTCTCTTCT TCAGGCCACA 
GAGTGGGCCT CCAGTGAGGA CACGCGCCGC TCCTGCCAAA GCAAAGGGAA GACTGAGGAG 
GAGTGTCAGA ACTACGTGCG AGTCCTGATC GTCGCCGGCC GGAAGGTGTT CATGTGTGGA 
ACCAATGCCT TTTCCCCCAT GTGCACCAGC AGACAGGTGG GGAACCTCAG CCGGACTATT 
GAGAAGATCA ATGGTGTGGC CCGCTGCCCC TATGACCCAC GCCACAACTC CACAGCTGTC 
ATCTCCTCCC AGGGGGAGCT CTATGCAGCC ACGGTCATCG ACTTCTCAGG TCGGGACCCT 
GCCATCTACC GCAGCCTGGG CAGTGGGCCA CCGCTTCGCA CTGCCCAATA TAACTCCAAG 
TGGCTTAATG AGCCAAACTT CGTGGCAGCC TATGATATTG GGCTGTTTGC ATACTTCTTC 
CTGCGGGAGA ACGCAGTGGA GCACGACTGT GGACGCACCG TGTACTCTCG CGTGGCCCGC 
GTGTGCAAGA ATGACGTGGG GGGCCGATTC CTGCTGGAGG ACACATGGAC CACATTCATG 
AAGGCCCGGC TCAACTGCTC CCGCCCGGGC GAGGTCCCCT TCTACTATAA CGAGCTGCAG 
AGTGCCTTCC ACTTGCCGGA GCAGGACCTC ATCTATGGAG TTTTCACAAC CAACGTAAAC 
AGCATCGCGG CTTCTGCTGT CTGCGCCTTC AACCTCAGTG CTATCTCCCA GGCTTTCAAT 
GGCCCATTTC GCTACCAGGA GAACCCCAGG GCTGCCTGGC TCCCCATAGC CAACCCCATC 
CCCAATTTCC AGTGTGGCAC CCTGCCTGAG ACCGGTCCCA ACGAGAACCT GACGGAGCGC 
AGCCTGCAGG ACGCGCAGCG CCTCTTCCTG ATGAGCGAGG CCGTGCAGCC GGTGACACCC 
GAGCCCTGTG TCACCCAGGA CAGCGTGCGC TTCTCACACC TCGTGGTGGA CCTGGTGCAG 
GCTAAAGACA CGCTCTACCA TGTACTCTAC ATTGGCACCG AGTCGGGCAC CATCCTGAAG 
GCGCTGTCCA CGGCGAGCCG CAGCCTCCAC GGCTGCTACC TGGAGGAGCT GCACGTGCTG 
CCCCCCGGGC GCCGCGAGCC CCTGCGCAGC CTGCGCATCC TGCACAGCGC CCGCGCGCTC 
TTCGTGGGGC TGAGAGACGG CGTCCTGCGG GTCCCACTGG AGAGGTGCGC CGCCTACCGC 
AGCCAGGGGG CATGCCTGGG GGCCCGGGAC CCGTACTGTG GCTGGGACGG GAAGCAGCAA 
CGTTGCAGCA CACTCGAGGA CAGCTCCAAC ATGAGCCTCT GGACCCAGAA CATCACCGCC 
TGTCCTGTGC GGAATGTGAC ACGGGATGGG GGCTTCGGCC CATGGTCACC ATGGCAACCA 
TGTGAGCACT TGGATGGGGA CAACTCAGGC TCTTGCCTGT GTCGAGCTCG ATCCTGTGAT 
TCCCCTCGAC CCCGCTGTGG GGGCCTTGAC TGCCTGGGGC CAGCCATCCA CATCGCCAAC 
TGCTCCAGGA ATGGGGCGTG GACCCCGTGG TCATCGTGGG CGCTGTGCAG CACGTCCTGT 
GGCATCGGCT TCCAGGTCCG CCAGCGAAGT TGCAGCAACC CTGCTCCCCG CCACGGGGGC 
CGCATCTGCG TGGGCAAGAG CCGGGAGGAA CGGTTCTGTA ATGAGAACAC GCCTTGCCCG 
GTGCCCATCT TCTGGGCTTC CTGGGGCTCC TGGAGCAAGT GCAGCAGCAA CTGTGGAGGG 
GGCATGCAGT CGCGGCGTCG GGCCTGCGAG AACGGCAACT CCTGCCTGGG CTGCGGCGTG 
GAGTTCAAGA CGTGCAACCC CGAGGGCTGC CCCGAAGTGC GGCGCAACAC CCCCTGGACG 
CCGTGGCTGC CCGTGAACGT GACGCAGGGC GGGGCACGGC AGGAGCAGCG GTTCCGCTTC 
ACCTGCCGCG CGCCCCTTGC AGACCCGCAC GGCCTGCAGT TCGGCAGGAG AAGGACCGAG 
ACGAGGACCT GTCCCGCGGA CGGCTCCGGC TCCTGCGACA CCGACGCCCT GGTGGAGGTC 
CTCCTGCGCA GCGGGAGCAC CTCCCCGCAC ACGGTGAGCG GGGGCTGGGC CGCCTGGGGC 
CCGTGGTCGT CCTGCTCCCG GGACTGCGAG CTGGGCTTCC GCGTCCGCAA GAGAACGTGC 
ACTAACCCGG AGCCCCGCAA CGGGGGCCTG CCCTGCGTGG GCGATGCTGC CGAGTACCAG 
GACTGCAACC CCCAGGCTTG CCCAGTTCGG GGTGCTTGGT CCTGCTGGAC CTCATGGTCT 
CCATGCTCAG CTTCCTGTGG TGGGGGTCAC TATCAACGCA CCCGTTCCTG CACCAGCCCC 
GCACCCTCCC CAGGTGAGGA CATCTGTCTC GGGCTGCACA CGGAGGAGGC ACTATGTGCC 
ACACAGGCCT GCCCAGAAGG CTGGTCGCCC TGGTCTGAGT GGAGTAAGTG CACTGACGAC 
GGAGCCCAGA GCCGAAGCCG GCACTGTGAG GAGCTCCTCC CAGGGTCCAG CGCCTGTGCT 
GGAAACAGCA GCCAGAGCCG CCCCTGCCCC TACAGCGAGA TTCCCGTCAT CCTGCCAGCC 
TCCAGCATGG AGGAGGCCAC CGACTGTGCA GGGTTCAATC TCATCCACTT GGTGGCCACG 
GGCATCTCCT GCTTCTTGGG CTCTGGGCTC CTGACCCTAG CAGTGTACCT GTCTTGCCAG 
CACTGCCAGC GTCAGTCCCA GGAGTCCACA CTGGTCCATC CTGCCACCCC CAACCATTTG 
CACTACAAGG GCGGAGGCAC CCCGAAGAAT GAAAAGTACA CACCCATGGA ATTCAAGACC 
CTGAACAAGA ATAACTTGAT CCCTGATGAC AGAGCCAACT TCTACCCATT GCAGCAGACC 
AATGTGTACA CGACTACTTA CTACCCAAGC CCCCTGAACA AACACAGCTT CCGGCCCGAG 
GCCTCACCTG GACAACGGTG CTTCCCCAAC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026872.2
CDS	388..3843
Translation	MPCGFSPSPVAHHLVPGPPDTPAQQLRCGWTVGGWLLSLVRGLLPCLPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCNPEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGWAAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIHLVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	NM_001031702.3
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 5B (SEMA5B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031702.3

ORF Insert Sequence:

ATGCCCTGTG GCTTCAGTCC GTCTCCTGTT GCCCACCACC TCGTCCCTGG GCCGCCTGAT 
ACCCCAGCCC AACAGCTAAG GTGTGGATGG ACAGTAGGGG GCTGGCTTCT CTCACTGGTC 
AGGGGTCTTC TCCCCTGTCT GCCTCCCGGA GCTAGGACTG CAGAGGGGCC TATCATGGTG 
CTTGCAGGCC CCCTGGCTGT CTCGCTGTTG CTGCCCAGCC TCACACTGCT GGTGTCCCAC 
CTCTCCAGCT CCCAGGATGT CTCCAGTGAG CCCAGCAGTG AGCAGCAGCT GTGCGCCCTT 
AGCAAGCACC CCACCGTGGC CTTTGAAGAC CTGCAGCCGT GGGTCTCTAA CTTCACCTAC 
CCTGGAGCCC GGGATTTCTC CCAGCTGGCT TTGGACCCCT CCGGGAACCA GCTCATCGTG 
GGAGCCAGGA ACTACCTCTT CAGACTCAGC CTTGCCAATG TCTCTCTTCT TCAGGCCACA 
GAGTGGGCCT CCAGTGAGGA CACGCGCCGC TCCTGCCAAA GCAAAGGGAA GACTGAGGAG 
GAGTGTCAGA ACTACGTGCG AGTCCTGATC GTCGCCGGCC GGAAGGTGTT CATGTGTGGA 
ACCAATGCCT TTTCCCCCAT GTGCACCAGC AGACAGGTGG GGAACCTCAG CCGGACTATT 
GAGAAGATCA ATGGTGTGGC CCGCTGCCCC TATGACCCAC GCCACAACTC CACAGCTGTC 
ATCTCCTCCC AGGGGGAGCT CTATGCAGCC ACGGTCATCG ACTTCTCAGG TCGGGACCCT 
GCCATCTACC GCAGCCTGGG CAGTGGGCCA CCGCTTCGCA CTGCCCAATA TAACTCCAAG 
TGGCTTAATG AGCCAAACTT CGTGGCAGCC TATGATATTG GGCTGTTTGC ATACTTCTTC 
CTGCGGGAGA ACGCAGTGGA GCACGACTGT GGACGCACCG TGTACTCTCG CGTGGCCCGC 
GTGTGCAAGA ATGACGTGGG GGGCCGATTC CTGCTGGAGG ACACATGGAC CACATTCATG 
AAGGCCCGGC TCAACTGCTC CCGCCCGGGC GAGGTCCCCT TCTACTATAA CGAGCTGCAG 
AGTGCCTTCC ACTTGCCGGA GCAGGACCTC ATCTATGGAG TTTTCACAAC CAACGTAAAC 
AGCATCGCGG CTTCTGCTGT CTGCGCCTTC AACCTCAGTG CTATCTCCCA GGCTTTCAAT 
GGCCCATTTC GCTACCAGGA GAACCCCAGG GCTGCCTGGC TCCCCATAGC CAACCCCATC 
CCCAATTTCC AGTGTGGCAC CCTGCCTGAG ACCGGTCCCA ACGAGAACCT GACGGAGCGC 
AGCCTGCAGG ACGCGCAGCG CCTCTTCCTG ATGAGCGAGG CCGTGCAGCC GGTGACACCC 
GAGCCCTGTG TCACCCAGGA CAGCGTGCGC TTCTCACACC TCGTGGTGGA CCTGGTGCAG 
GCTAAAGACA CGCTCTACCA TGTACTCTAC ATTGGCACCG AGTCGGGCAC CATCCTGAAG 
GCGCTGTCCA CGGCGAGCCG CAGCCTCCAC GGCTGCTACC TGGAGGAGCT GCACGTGCTG 
CCCCCCGGGC GCCGCGAGCC CCTGCGCAGC CTGCGCATCC TGCACAGCGC CCGCGCGCTC 
TTCGTGGGGC TGAGAGACGG CGTCCTGCGG GTCCCACTGG AGAGGTGCGC CGCCTACCGC 
AGCCAGGGGG CATGCCTGGG GGCCCGGGAC CCGTACTGTG GCTGGGACGG GAAGCAGCAA 
CGTTGCAGCA CACTCGAGGA CAGCTCCAAC ATGAGCCTCT GGACCCAGAA CATCACCGCC 
TGTCCTGTGC GGAATGTGAC ACGGGATGGG GGCTTCGGCC CATGGTCACC ATGGCAACCA 
TGTGAGCACT TGGATGGGGA CAACTCAGGC TCTTGCCTGT GTCGAGCTCG ATCCTGTGAT 
TCCCCTCGAC CCCGCTGTGG GGGCCTTGAC TGCCTGGGGC CAGCCATCCA CATCGCCAAC 
TGCTCCAGGA ATGGGGCGTG GACCCCGTGG TCATCGTGGG CGCTGTGCAG CACGTCCTGT 
GGCATCGGCT TCCAGGTCCG CCAGCGAAGT TGCAGCAACC CTGCTCCCCG CCACGGGGGC 
CGCATCTGCG TGGGCAAGAG CCGGGAGGAA CGGTTCTGTA ATGAGAACAC GCCTTGCCCG 
GTGCCCATCT TCTGGGCTTC CTGGGGCTCC TGGAGCAAGT GCAGCAGCAA CTGTGGAGGG 
GGCATGCAGT CGCGGCGTCG GGCCTGCGAG AACGGCAACT CCTGCCTGGG CTGCGGCGTG 
GAGTTCAAGA CGTGCAACCC CGAGGGCTGC CCCGAAGTGC GGCGCAACAC CCCCTGGACG 
CCGTGGCTGC CCGTGAACGT GACGCAGGGC GGGGCACGGC AGGAGCAGCG GTTCCGCTTC 
ACCTGCCGCG CGCCCCTTGC AGACCCGCAC GGCCTGCAGT TCGGCAGGAG AAGGACCGAG 
ACGAGGACCT GTCCCGCGGA CGGCTCCGGC TCCTGCGACA CCGACGCCCT GGTGGAGGTC 
CTCCTGCGCA GCGGGAGCAC CTCCCCGCAC ACGGTGAGCG GGGGCTGGGC CGCCTGGGGC 
CCGTGGTCGT CCTGCTCCCG GGACTGCGAG CTGGGCTTCC GCGTCCGCAA GAGAACGTGC 
ACTAACCCGG AGCCCCGCAA CGGGGGCCTG CCCTGCGTGG GCGATGCTGC CGAGTACCAG 
GACTGCAACC CCCAGGCTTG CCCAGTTCGG GGTGCTTGGT CCTGCTGGAC CTCATGGTCT 
CCATGCTCAG CTTCCTGTGG TGGGGGTCAC TATCAACGCA CCCGTTCCTG CACCAGCCCC 
GCACCCTCCC CAGGTGAGGA CATCTGTCTC GGGCTGCACA CGGAGGAGGC ACTATGTGCC 
ACACAGGCCT GCCCAGAAGG CTGGTCGCCC TGGTCTGAGT GGAGTAAGTG CACTGACGAC 
GGAGCCCAGA GCCGAAGCCG GCACTGTGAG GAGCTCCTCC CAGGGTCCAG CGCCTGTGCT 
GGAAACAGCA GCCAGAGCCG CCCCTGCCCC TACAGCGAGA TTCCCGTCAT CCTGCCAGCC 
TCCAGCATGG AGGAGGCCAC CGACTGTGCA GGGTTCAATC TCATCCACTT GGTGGCCACG 
GGCATCTCCT GCTTCTTGGG CTCTGGGCTC CTGACCCTAG CAGTGTACCT GTCTTGCCAG 
CACTGCCAGC GTCAGTCCCA GGAGTCCACA CTGGTCCATC CTGCCACCCC CAACCATTTG 
CACTACAAGG GCGGAGGCAC CCCGAAGAAT GAAAAGTACA CACCCATGGA ATTCAAGACC 
CTGAACAAGA ATAACTTGAT CCCTGATGAC AGAGCCAACT TCTACCCATT GCAGCAGACC 
AATGTGTACA CGACTACTTA CTACCCAAGC CCCCTGAACA AACACAGCTT CCGGCCCGAG 
GCCTCACCTG GACAACGGTG CTTCCCCAAC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5B ( NM_001256346.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243275.1 Homo sapiens semaphorin 5B (SEMA5B), transcript variant 2, mRNA.

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CloneID	OHu01368
Clone ID Related Accession (Same CDS sequence)	NM_001031702.4 , NM_001031702.3 , NM_001256346.1
Accession Version	NM_001256346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Homo sapiens(human)
Product	semaphorin-5B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK091481.1, AC109130.8 and AC078794.13. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## CDS exon combination :: AB040878.1, BC077726.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCCCTGTG GCTTCAGTCC GTCTCCTGTT GCCCACCACC TCGTCCCTGG GCCGCCTGAT 
ACCCCAGCCC AACAGCTAAG GTGTGGATGG ACAGTAGGGG GCTGGCTTCT CTCACTGGTC 
AGGGGTCTTC TCCCCTGTCT GCCTCCCGGA GCTAGGACTG CAGAGGGGCC TATCATGGTG 
CTTGCAGGCC CCCTGGCTGT CTCGCTGTTG CTGCCCAGCC TCACACTGCT GGTGTCCCAC 
CTCTCCAGCT CCCAGGATGT CTCCAGTGAG CCCAGCAGTG AGCAGCAGCT GTGCGCCCTT 
AGCAAGCACC CCACCGTGGC CTTTGAAGAC CTGCAGCCGT GGGTCTCTAA CTTCACCTAC 
CCTGGAGCCC GGGATTTCTC CCAGCTGGCT TTGGACCCCT CCGGGAACCA GCTCATCGTG 
GGAGCCAGGA ACTACCTCTT CAGACTCAGC CTTGCCAATG TCTCTCTTCT TCAGGCCACA 
GAGTGGGCCT CCAGTGAGGA CACGCGCCGC TCCTGCCAAA GCAAAGGGAA GACTGAGGAG 
GAGTGTCAGA ACTACGTGCG AGTCCTGATC GTCGCCGGCC GGAAGGTGTT CATGTGTGGA 
ACCAATGCCT TTTCCCCCAT GTGCACCAGC AGACAGGTGG GGAACCTCAG CCGGACTATT 
GAGAAGATCA ATGGTGTGGC CCGCTGCCCC TATGACCCAC GCCACAACTC CACAGCTGTC 
ATCTCCTCCC AGGGGGAGCT CTATGCAGCC ACGGTCATCG ACTTCTCAGG TCGGGACCCT 
GCCATCTACC GCAGCCTGGG CAGTGGGCCA CCGCTTCGCA CTGCCCAATA TAACTCCAAG 
TGGCTTAATG AGCCAAACTT CGTGGCAGCC TATGATATTG GGCTGTTTGC ATACTTCTTC 
CTGCGGGAGA ACGCAGTGGA GCACGACTGT GGACGCACCG TGTACTCTCG CGTGGCCCGC 
GTGTGCAAGA ATGACGTGGG GGGCCGATTC CTGCTGGAGG ACACATGGAC CACATTCATG 
AAGGCCCGGC TCAACTGCTC CCGCCCGGGC GAGGTCCCCT TCTACTATAA CGAGCTGCAG 
AGTGCCTTCC ACTTGCCGGA GCAGGACCTC ATCTATGGAG TTTTCACAAC CAACGTAAAC 
AGCATCGCGG CTTCTGCTGT CTGCGCCTTC AACCTCAGTG CTATCTCCCA GGCTTTCAAT 
GGCCCATTTC GCTACCAGGA GAACCCCAGG GCTGCCTGGC TCCCCATAGC CAACCCCATC 
CCCAATTTCC AGTGTGGCAC CCTGCCTGAG ACCGGTCCCA ACGAGAACCT GACGGAGCGC 
AGCCTGCAGG ACGCGCAGCG CCTCTTCCTG ATGAGCGAGG CCGTGCAGCC GGTGACACCC 
GAGCCCTGTG TCACCCAGGA CAGCGTGCGC TTCTCACACC TCGTGGTGGA CCTGGTGCAG 
GCTAAAGACA CGCTCTACCA TGTACTCTAC ATTGGCACCG AGTCGGGCAC CATCCTGAAG 
GCGCTGTCCA CGGCGAGCCG CAGCCTCCAC GGCTGCTACC TGGAGGAGCT GCACGTGCTG 
CCCCCCGGGC GCCGCGAGCC CCTGCGCAGC CTGCGCATCC TGCACAGCGC CCGCGCGCTC 
TTCGTGGGGC TGAGAGACGG CGTCCTGCGG GTCCCACTGG AGAGGTGCGC CGCCTACCGC 
AGCCAGGGGG CATGCCTGGG GGCCCGGGAC CCGTACTGTG GCTGGGACGG GAAGCAGCAA 
CGTTGCAGCA CACTCGAGGA CAGCTCCAAC ATGAGCCTCT GGACCCAGAA CATCACCGCC 
TGTCCTGTGC GGAATGTGAC ACGGGATGGG GGCTTCGGCC CATGGTCACC ATGGCAACCA 
TGTGAGCACT TGGATGGGGA CAACTCAGGC TCTTGCCTGT GTCGAGCTCG ATCCTGTGAT 
TCCCCTCGAC CCCGCTGTGG GGGCCTTGAC TGCCTGGGGC CAGCCATCCA CATCGCCAAC 
TGCTCCAGGA ATGGGGCGTG GACCCCGTGG TCATCGTGGG CGCTGTGCAG CACGTCCTGT 
GGCATCGGCT TCCAGGTCCG CCAGCGAAGT TGCAGCAACC CTGCTCCCCG CCACGGGGGC 
CGCATCTGCG TGGGCAAGAG CCGGGAGGAA CGGTTCTGTA ATGAGAACAC GCCTTGCCCG 
GTGCCCATCT TCTGGGCTTC CTGGGGCTCC TGGAGCAAGT GCAGCAGCAA CTGTGGAGGG 
GGCATGCAGT CGCGGCGTCG GGCCTGCGAG AACGGCAACT CCTGCCTGGG CTGCGGCGTG 
GAGTTCAAGA CGTGCAACCC CGAGGGCTGC CCCGAAGTGC GGCGCAACAC CCCCTGGACG 
CCGTGGCTGC CCGTGAACGT GACGCAGGGC GGGGCACGGC AGGAGCAGCG GTTCCGCTTC 
ACCTGCCGCG CGCCCCTTGC AGACCCGCAC GGCCTGCAGT TCGGCAGGAG AAGGACCGAG 
ACGAGGACCT GTCCCGCGGA CGGCTCCGGC TCCTGCGACA CCGACGCCCT GGTGGAGGTC 
CTCCTGCGCA GCGGGAGCAC CTCCCCGCAC ACGGTGAGCG GGGGCTGGGC CGCCTGGGGC 
CCGTGGTCGT CCTGCTCCCG GGACTGCGAG CTGGGCTTCC GCGTCCGCAA GAGAACGTGC 
ACTAACCCGG AGCCCCGCAA CGGGGGCCTG CCCTGCGTGG GCGATGCTGC CGAGTACCAG 
GACTGCAACC CCCAGGCTTG CCCAGTTCGG GGTGCTTGGT CCTGCTGGAC CTCATGGTCT 
CCATGCTCAG CTTCCTGTGG TGGGGGTCAC TATCAACGCA CCCGTTCCTG CACCAGCCCC 
GCACCCTCCC CAGGTGAGGA CATCTGTCTC GGGCTGCACA CGGAGGAGGC ACTATGTGCC 
ACACAGGCCT GCCCAGAAGG CTGGTCGCCC TGGTCTGAGT GGAGTAAGTG CACTGACGAC 
GGAGCCCAGA GCCGAAGCCG GCACTGTGAG GAGCTCCTCC CAGGGTCCAG CGCCTGTGCT 
GGAAACAGCA GCCAGAGCCG CCCCTGCCCC TACAGCGAGA TTCCCGTCAT CCTGCCAGCC 
TCCAGCATGG AGGAGGCCAC CGACTGTGCA GGGTTCAATC TCATCCACTT GGTGGCCACG 
GGCATCTCCT GCTTCTTGGG CTCTGGGCTC CTGACCCTAG CAGTGTACCT GTCTTGCCAG 
CACTGCCAGC GTCAGTCCCA GGAGTCCACA CTGGTCCATC CTGCCACCCC CAACCATTTG 
CACTACAAGG GCGGAGGCAC CCCGAAGAAT GAAAAGTACA CACCCATGGA ATTCAAGACC 
CTGAACAAGA ATAACTTGAT CCCTGATGAC AGAGCCAACT TCTACCCATT GCAGCAGACC 
AATGTGTACA CGACTACTTA CTACCCAAGC CCCCTGAACA AACACAGCTT CCGGCCCGAG 
GCCTCACCTG GACAACGGTG CTTCCCCAAC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243275.1
CDS	528..3983
Translation	MPCGFSPSPVAHHLVPGPPDTPAQQLRCGWTVGGWLLSLVRGLLPCLPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESGTILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLEDSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALCSTSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCNPEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGWAAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIHLVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKHSFRPEASPGQRCFPNS

Target ORF information:

RefSeq Version	NM_001256346.1
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 5B (SEMA5B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256346.1

ORF Insert Sequence:

ATGCCCTGTG GCTTCAGTCC GTCTCCTGTT GCCCACCACC TCGTCCCTGG GCCGCCTGAT 
ACCCCAGCCC AACAGCTAAG GTGTGGATGG ACAGTAGGGG GCTGGCTTCT CTCACTGGTC 
AGGGGTCTTC TCCCCTGTCT GCCTCCCGGA GCTAGGACTG CAGAGGGGCC TATCATGGTG 
CTTGCAGGCC CCCTGGCTGT CTCGCTGTTG CTGCCCAGCC TCACACTGCT GGTGTCCCAC 
CTCTCCAGCT CCCAGGATGT CTCCAGTGAG CCCAGCAGTG AGCAGCAGCT GTGCGCCCTT 
AGCAAGCACC CCACCGTGGC CTTTGAAGAC CTGCAGCCGT GGGTCTCTAA CTTCACCTAC 
CCTGGAGCCC GGGATTTCTC CCAGCTGGCT TTGGACCCCT CCGGGAACCA GCTCATCGTG 
GGAGCCAGGA ACTACCTCTT CAGACTCAGC CTTGCCAATG TCTCTCTTCT TCAGGCCACA 
GAGTGGGCCT CCAGTGAGGA CACGCGCCGC TCCTGCCAAA GCAAAGGGAA GACTGAGGAG 
GAGTGTCAGA ACTACGTGCG AGTCCTGATC GTCGCCGGCC GGAAGGTGTT CATGTGTGGA 
ACCAATGCCT TTTCCCCCAT GTGCACCAGC AGACAGGTGG GGAACCTCAG CCGGACTATT 
GAGAAGATCA ATGGTGTGGC CCGCTGCCCC TATGACCCAC GCCACAACTC CACAGCTGTC 
ATCTCCTCCC AGGGGGAGCT CTATGCAGCC ACGGTCATCG ACTTCTCAGG TCGGGACCCT 
GCCATCTACC GCAGCCTGGG CAGTGGGCCA CCGCTTCGCA CTGCCCAATA TAACTCCAAG 
TGGCTTAATG AGCCAAACTT CGTGGCAGCC TATGATATTG GGCTGTTTGC ATACTTCTTC 
CTGCGGGAGA ACGCAGTGGA GCACGACTGT GGACGCACCG TGTACTCTCG CGTGGCCCGC 
GTGTGCAAGA ATGACGTGGG GGGCCGATTC CTGCTGGAGG ACACATGGAC CACATTCATG 
AAGGCCCGGC TCAACTGCTC CCGCCCGGGC GAGGTCCCCT TCTACTATAA CGAGCTGCAG 
AGTGCCTTCC ACTTGCCGGA GCAGGACCTC ATCTATGGAG TTTTCACAAC CAACGTAAAC 
AGCATCGCGG CTTCTGCTGT CTGCGCCTTC AACCTCAGTG CTATCTCCCA GGCTTTCAAT 
GGCCCATTTC GCTACCAGGA GAACCCCAGG GCTGCCTGGC TCCCCATAGC CAACCCCATC 
CCCAATTTCC AGTGTGGCAC CCTGCCTGAG ACCGGTCCCA ACGAGAACCT GACGGAGCGC 
AGCCTGCAGG ACGCGCAGCG CCTCTTCCTG ATGAGCGAGG CCGTGCAGCC GGTGACACCC 
GAGCCCTGTG TCACCCAGGA CAGCGTGCGC TTCTCACACC TCGTGGTGGA CCTGGTGCAG 
GCTAAAGACA CGCTCTACCA TGTACTCTAC ATTGGCACCG AGTCGGGCAC CATCCTGAAG 
GCGCTGTCCA CGGCGAGCCG CAGCCTCCAC GGCTGCTACC TGGAGGAGCT GCACGTGCTG 
CCCCCCGGGC GCCGCGAGCC CCTGCGCAGC CTGCGCATCC TGCACAGCGC CCGCGCGCTC 
TTCGTGGGGC TGAGAGACGG CGTCCTGCGG GTCCCACTGG AGAGGTGCGC CGCCTACCGC 
AGCCAGGGGG CATGCCTGGG GGCCCGGGAC CCGTACTGTG GCTGGGACGG GAAGCAGCAA 
CGTTGCAGCA CACTCGAGGA CAGCTCCAAC ATGAGCCTCT GGACCCAGAA CATCACCGCC 
TGTCCTGTGC GGAATGTGAC ACGGGATGGG GGCTTCGGCC CATGGTCACC ATGGCAACCA 
TGTGAGCACT TGGATGGGGA CAACTCAGGC TCTTGCCTGT GTCGAGCTCG ATCCTGTGAT 
TCCCCTCGAC CCCGCTGTGG GGGCCTTGAC TGCCTGGGGC CAGCCATCCA CATCGCCAAC 
TGCTCCAGGA ATGGGGCGTG GACCCCGTGG TCATCGTGGG CGCTGTGCAG CACGTCCTGT 
GGCATCGGCT TCCAGGTCCG CCAGCGAAGT TGCAGCAACC CTGCTCCCCG CCACGGGGGC 
CGCATCTGCG TGGGCAAGAG CCGGGAGGAA CGGTTCTGTA ATGAGAACAC GCCTTGCCCG 
GTGCCCATCT TCTGGGCTTC CTGGGGCTCC TGGAGCAAGT GCAGCAGCAA CTGTGGAGGG 
GGCATGCAGT CGCGGCGTCG GGCCTGCGAG AACGGCAACT CCTGCCTGGG CTGCGGCGTG 
GAGTTCAAGA CGTGCAACCC CGAGGGCTGC CCCGAAGTGC GGCGCAACAC CCCCTGGACG 
CCGTGGCTGC CCGTGAACGT GACGCAGGGC GGGGCACGGC AGGAGCAGCG GTTCCGCTTC 
ACCTGCCGCG CGCCCCTTGC AGACCCGCAC GGCCTGCAGT TCGGCAGGAG AAGGACCGAG 
ACGAGGACCT GTCCCGCGGA CGGCTCCGGC TCCTGCGACA CCGACGCCCT GGTGGAGGTC 
CTCCTGCGCA GCGGGAGCAC CTCCCCGCAC ACGGTGAGCG GGGGCTGGGC CGCCTGGGGC 
CCGTGGTCGT CCTGCTCCCG GGACTGCGAG CTGGGCTTCC GCGTCCGCAA GAGAACGTGC 
ACTAACCCGG AGCCCCGCAA CGGGGGCCTG CCCTGCGTGG GCGATGCTGC CGAGTACCAG 
GACTGCAACC CCCAGGCTTG CCCAGTTCGG GGTGCTTGGT CCTGCTGGAC CTCATGGTCT 
CCATGCTCAG CTTCCTGTGG TGGGGGTCAC TATCAACGCA CCCGTTCCTG CACCAGCCCC 
GCACCCTCCC CAGGTGAGGA CATCTGTCTC GGGCTGCACA CGGAGGAGGC ACTATGTGCC 
ACACAGGCCT GCCCAGAAGG CTGGTCGCCC TGGTCTGAGT GGAGTAAGTG CACTGACGAC 
GGAGCCCAGA GCCGAAGCCG GCACTGTGAG GAGCTCCTCC CAGGGTCCAG CGCCTGTGCT 
GGAAACAGCA GCCAGAGCCG CCCCTGCCCC TACAGCGAGA TTCCCGTCAT CCTGCCAGCC 
TCCAGCATGG AGGAGGCCAC CGACTGTGCA GGGTTCAATC TCATCCACTT GGTGGCCACG 
GGCATCTCCT GCTTCTTGGG CTCTGGGCTC CTGACCCTAG CAGTGTACCT GTCTTGCCAG 
CACTGCCAGC GTCAGTCCCA GGAGTCCACA CTGGTCCATC CTGCCACCCC CAACCATTTG 
CACTACAAGG GCGGAGGCAC CCCGAAGAAT GAAAAGTACA CACCCATGGA ATTCAAGACC 
CTGAACAAGA ATAACTTGAT CCCTGATGAC AGAGCCAACT TCTACCCATT GCAGCAGACC 
AATGTGTACA CGACTACTTA CTACCCAAGC CCCCTGAACA AACACAGCTT CCGGCCCGAG 
GCCTCACCTG GACAACGGTG CTTCCCCAAC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Driver Gene and Novel Mutations in Asbestos-Exposed Lung Adenocarcinoma and Malignant Mesothelioma Detected by Exome Sequencing.
Lung194(1)125-35(2016 Feb)
M?ki-Nevala S,Sarhadi VK,Knuuttila A,Scheinin I,Ellonen P,Lagstr?m S,R?nty M,Kettunen E,Husgafvel-Pursiainen K,Wolff H,Knuutila S

Genome-wide association study identifies three new susceptibility loci for esophageal squamous-cell carcinoma in Chinese populations.
Nature genetics43(7)679-84(2011 Jun)
Wu C,Hu Z,He Z,Jia W,Wang F,Zhou Y,Liu Z,Zhan Q,Liu Y,Yu D,Zhai K,Chang J,Qiao Y,Jin G,Liu Z,Shen Y,Guo C,Fu J,Miao X,Tan W,Shen H,Ke Y,Zeng Y,Wu T,Lin D

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SEMA5B cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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