SEMA5B cDNA ORF clone, Homo sapiens(human)

The following SEMA5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu00289 NM_001256347.1
Latest version!
Homo sapiens semaphorin 5B (SEMA5B), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu94293 XM_017006639.1
Latest version!
Homo sapiens semaphorin 5B (SEMA5B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu94292 XM_017006638.2
Latest version!
Homo sapiens semaphorin 5B (SEMA5B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu00906 NM_001256348.1
Latest version!
Homo sapiens semaphorin 5B (SEMA5B), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu01368 NM_001256346.1
Latest version!
Homo sapiens semaphorin 5B (SEMA5B), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu01368 NM_001031702.4
Latest version!
Homo sapiens semaphorin 5B (SEMA5B), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
View Old Accession Versions >>
OHu01368 NM_001031702.3 Homo sapiens semaphorin 5B (SEMA5B), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu94292 XM_017006638.1 Homo sapiens semaphorin 5B (SEMA5B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu00289
    Clone ID Related Accession (Same CDS sequence) NM_001256347.1
    Accession Version NM_001256347.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-22
    Organism Homo sapiens(human)
    Product semaphorin-5B isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK295619.1, AC109130.8 and AC078794.13. Transcript Variant: This variant (3) differs in the 5' UTR and coding region and uses an alternate start codon compared to variant 1. The resulting protein (isoform 2) has a longer N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK295619.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGCATC TGTCAGCAGA GGAAGCCATT GGCTGCGTGA GGGTCAGAAG GAGCTTCATA 
    GATGAGCTGG CATTTGGGAG AGGGCATTCT ACAGGGACGG GCAAGCAGAA GCGAAGGGAT
    CGAGTGAGTG GTTCCAGCTG GTGCCTGGCC TGTGTCTCTT GGATGCCCTG TGGCTTCAGT
    CCGTCTCCTG TTGCCCACCA CCTCGTCCCT GGGCCGCCTG ATACCCCAGC CCAACAGCTA
    AGGTGTGGAT GGACAGTAGG GGGCTGGCTT CTCTCACTGG TCAGGGGTCT TCTCCCCTGT
    CTGCCTCCCG GAGCTAGGAC TGCAGAGGGG CCTATCATGG TGCTTGCAGG CCCCCTGGCT
    GTCTCGCTGT TGCTGCCCAG CCTCACACTG CTGGTGTCCC ACCTCTCCAG CTCCCAGGAT
    GTCTCCAGTG AGCCCAGCAG TGAGCAGCAG CTGTGCGCCC TTAGCAAGCA CCCCACCGTG
    GCCTTTGAAG ACCTGCAGCC GTGGGTCTCT AACTTCACCT ACCCTGGAGC CCGGGATTTC
    TCCCAGCTGG CTTTGGACCC CTCCGGGAAC CAGCTCATCG TGGGAGCCAG GAACTACCTC
    TTCAGACTCA GCCTTGCCAA TGTCTCTCTT CTTCAGGCCA CAGAGTGGGC CTCCAGTGAG
    GACACGCGCC GCTCCTGCCA AAGCAAAGGG AAGACTGAGG AGGAGTGTCA GAACTACGTG
    CGAGTCCTGA TCGTCGCCGG CCGGAAGGTG TTCATGTGTG GAACCAATGC CTTTTCCCCC
    ATGTGCACCA GCAGACAGGT GGGGAACCTC AGCCGGACTA TTGAGAAGAT CAATGGTGTG
    GCCCGCTGCC CCTATGACCC ACGCCACAAC TCCACAGCTG TCATCTCCTC CCAGGGGGAG
    CTCTATGCAG CCACGGTCAT CGACTTCTCA GGTCGGGACC CTGCCATCTA CCGCAGCCTG
    GGCAGTGGGC CACCGCTTCG CACTGCCCAA TATAACTCCA AGTGGCTTAA TGAGCCAAAC
    TTCGTGGCAG CCTATGATAT TGGGCTGTTT GCATACTTCT TCCTGCGGGA GAACGCAGTG
    GAGCACGACT GTGGACGCAC CGTGTACTCT CGCGTGGCCC GCGTGTGCAA GAATGACGTG
    GGGGGCCGAT TCCTGCTGGA GGACACATGG ACCACATTCA TGAAGGCCCG GCTCAACTGC
    TCCCGCCCGG GCGAGGTCCC CTTCTACTAT AACGAGCTGC AGAGTGCCTT CCACTTGCCG
    GAGCAGGACC TCATCTATGG AGTTTTCACA ACCAACGTAA ACAGCATCGC GGCTTCTGCT
    GTCTGCGCCT TCAACCTCAG TGCTATCTCC CAGGCTTTCA ATGGCCCATT TCGCTACCAG
    GAGAACCCCA GGGCTGCCTG GCTCCCCATA GCCAACCCCA TCCCCAATTT CCAGTGTGGC
    ACCCTGCCTG AGACCGGTCC CAACGAGAAC CTGACGGAGC GCAGCCTGCA GGACGCGCAG
    CGCCTCTTCC TGATGAGCGA GGCCGTGCAG CCGGTGACAC CCGAGCCCTG TGTCACCCAG
    GACAGCGTGC GCTTCTCACA CCTCGTGGTG GACCTGGTGC AGGCTAAAGA CACGCTCTAC
    CATGTACTCT ACATTGGCAC CGAGTCGGGC ACCATCCTGA AGGCGCTGTC CACGGCGAGC
    CGCAGCCTCC ACGGCTGCTA CCTGGAGGAG CTGCACGTGC TGCCCCCCGG GCGCCGCGAG
    CCCCTGCGCA GCCTGCGCAT CCTGCACAGC GCCCGCGCGC TCTTCGTGGG GCTGAGAGAC
    GGCGTCCTGC GGGTCCCACT GGAGAGGTGC GCCGCCTACC GCAGCCAGGG GGCATGCCTG
    GGGGCCCGGG ACCCGTACTG TGGCTGGGAC GGGAAGCAGC AACGTTGCAG CACACTCGAG
    GACAGCTCCA ACATGAGCCT CTGGACCCAG AACATCACCG CCTGTCCTGT GCGGAATGTG
    ACACGGGATG GGGGCTTCGG CCCATGGTCA CCATGGCAAC CATGTGAGCA CTTGGATGGG
    GACAACTCAG GCTCTTGCCT GTGTCGAGCT CGATCCTGTG ATTCCCCTCG ACCCCGCTGT
    GGGGGCCTTG ACTGCCTGGG GCCAGCCATC CACATCGCCA ACTGCTCCAG GAATGGGGCG
    TGGACCCCGT GGTCATCGTG GGCGCTGTGC AGCACGTCCT GTGGCATCGG CTTCCAGGTC
    CGCCAGCGAA GTTGCAGCAA CCCTGCTCCC CGCCACGGGG GCCGCATCTG CGTGGGCAAG
    AGCCGGGAGG AACGGTTCTG TAATGAGAAC ACGCCTTGCC CGGTGCCCAT CTTCTGGGCT
    TCCTGGGGCT CCTGGAGCAA GTGCAGCAGC AACTGTGGAG GGGGCATGCA GTCGCGGCGT
    CGGGCCTGCG AGAACGGCAA CTCCTGCCTG GGCTGCGGCG TGGAGTTCAA GACGTGCAAC
    CCCGAGGGCT GCCCCGAAGT GCGGCGCAAC ACCCCCTGGA CGCCGTGGCT GCCCGTGAAC
    GTGACGCAGG GCGGGGCACG GCAGGAGCAG CGGTTCCGCT TCACCTGCCG CGCGCCCCTT
    GCAGACCCGC ACGGCCTGCA GTTCGGCAGG AGAAGGACCG AGACGAGGAC CTGTCCCGCG
    GACGGCTCCG GCTCCTGCGA CACCGACGCC CTGGTGGAGG TCCTCCTGCG CAGCGGGAGC
    ACCTCCCCGC ACACGGTGAG CGGGGGCTGG GCCGCCTGGG GCCCGTGGTC GTCCTGCTCC
    CGGGACTGCG AGCTGGGCTT CCGCGTCCGC AAGAGAACGT GCACTAACCC GGAGCCCCGC
    AACGGGGGCC TGCCCTGCGT GGGCGATGCT GCCGAGTACC AGGACTGCAA CCCCCAGGCT
    TGCCCAGTTC GGGGTGCTTG GTCCTGCTGG ACCTCATGGT CTCCATGCTC AGCTTCCTGT
    GGTGGGGGTC ACTATCAACG CACCCGTTCC TGCACCAGCC CCGCACCCTC CCCAGGTGAG
    GACATCTGTC TCGGGCTGCA CACGGAGGAG GCACTATGTG CCACACAGGC CTGCCCAGAA
    GGCTGGTCGC CCTGGTCTGA GTGGAGTAAG TGCACTGACG ACGGAGCCCA GAGCCGAAGC
    CGGCACTGTG AGGAGCTCCT CCCAGGGTCC AGCGCCTGTG CTGGAAACAG CAGCCAGAGC
    CGCCCCTGCC CCTACAGCGA GATTCCCGTC ATCCTGCCAG CCTCCAGCAT GGAGGAGGCC
    ACCGACTGTG CAGGGTTCAA TCTCATCCAC TTGGTGGCCA CGGGCATCTC CTGCTTCTTG
    GGCTCTGGGC TCCTGACCCT AGCAGTGTAC CTGTCTTGCC AGCACTGCCA GCGTCAGTCC
    CAGGAGTCCA CACTGGTCCA TCCTGCCACC CCCAACCATT TGCACTACAA GGGCGGAGGC
    ACCCCGAAGA ATGAAAAGTA CACACCCATG GAATTCAAGA CCCTGAACAA GAATAACTTG
    ATCCCTGATG ACAGAGCCAA CTTCTACCCA TTGCAGCAGA CCAATGTGTA CACGACTACT
    TACTACCCAA GCCCCCTGAA CAAACACAGC TTCCGGCCCG AGGCCTCACC TGGACAACGG
    TGCTTCCCCA ACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243276.1
    CDS12..3629
    Translation

    Target ORF information:

    RefSeq Version NM_001256347.1
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5B (SEMA5B), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTGCATC TGTCAGCAGA GGAAGCCATT GGCTGCGTGA GGGTCAGAAG GAGCTTCATA 
    GATGAGCTGG CATTTGGGAG AGGGCATTCT ACAGGGACGG GCAAGCAGAA GCGAAGGGAT
    CGAGTGAGTG GTTCCAGCTG GTGCCTGGCC TGTGTCTCTT GGATGCCCTG TGGCTTCAGT
    CCGTCTCCTG TTGCCCACCA CCTCGTCCCT GGGCCGCCTG ATACCCCAGC CCAACAGCTA
    AGGTGTGGAT GGACAGTAGG GGGCTGGCTT CTCTCACTGG TCAGGGGTCT TCTCCCCTGT
    CTGCCTCCCG GAGCTAGGAC TGCAGAGGGG CCTATCATGG TGCTTGCAGG CCCCCTGGCT
    GTCTCGCTGT TGCTGCCCAG CCTCACACTG CTGGTGTCCC ACCTCTCCAG CTCCCAGGAT
    GTCTCCAGTG AGCCCAGCAG TGAGCAGCAG CTGTGCGCCC TTAGCAAGCA CCCCACCGTG
    GCCTTTGAAG ACCTGCAGCC GTGGGTCTCT AACTTCACCT ACCCTGGAGC CCGGGATTTC
    TCCCAGCTGG CTTTGGACCC CTCCGGGAAC CAGCTCATCG TGGGAGCCAG GAACTACCTC
    TTCAGACTCA GCCTTGCCAA TGTCTCTCTT CTTCAGGCCA CAGAGTGGGC CTCCAGTGAG
    GACACGCGCC GCTCCTGCCA AAGCAAAGGG AAGACTGAGG AGGAGTGTCA GAACTACGTG
    CGAGTCCTGA TCGTCGCCGG CCGGAAGGTG TTCATGTGTG GAACCAATGC CTTTTCCCCC
    ATGTGCACCA GCAGACAGGT GGGGAACCTC AGCCGGACTA TTGAGAAGAT CAATGGTGTG
    GCCCGCTGCC CCTATGACCC ACGCCACAAC TCCACAGCTG TCATCTCCTC CCAGGGGGAG
    CTCTATGCAG CCACGGTCAT CGACTTCTCA GGTCGGGACC CTGCCATCTA CCGCAGCCTG
    GGCAGTGGGC CACCGCTTCG CACTGCCCAA TATAACTCCA AGTGGCTTAA TGAGCCAAAC
    TTCGTGGCAG CCTATGATAT TGGGCTGTTT GCATACTTCT TCCTGCGGGA GAACGCAGTG
    GAGCACGACT GTGGACGCAC CGTGTACTCT CGCGTGGCCC GCGTGTGCAA GAATGACGTG
    GGGGGCCGAT TCCTGCTGGA GGACACATGG ACCACATTCA TGAAGGCCCG GCTCAACTGC
    TCCCGCCCGG GCGAGGTCCC CTTCTACTAT AACGAGCTGC AGAGTGCCTT CCACTTGCCG
    GAGCAGGACC TCATCTATGG AGTTTTCACA ACCAACGTAA ACAGCATCGC GGCTTCTGCT
    GTCTGCGCCT TCAACCTCAG TGCTATCTCC CAGGCTTTCA ATGGCCCATT TCGCTACCAG
    GAGAACCCCA GGGCTGCCTG GCTCCCCATA GCCAACCCCA TCCCCAATTT CCAGTGTGGC
    ACCCTGCCTG AGACCGGTCC CAACGAGAAC CTGACGGAGC GCAGCCTGCA GGACGCGCAG
    CGCCTCTTCC TGATGAGCGA GGCCGTGCAG CCGGTGACAC CCGAGCCCTG TGTCACCCAG
    GACAGCGTGC GCTTCTCACA CCTCGTGGTG GACCTGGTGC AGGCTAAAGA CACGCTCTAC
    CATGTACTCT ACATTGGCAC CGAGTCGGGC ACCATCCTGA AGGCGCTGTC CACGGCGAGC
    CGCAGCCTCC ACGGCTGCTA CCTGGAGGAG CTGCACGTGC TGCCCCCCGG GCGCCGCGAG
    CCCCTGCGCA GCCTGCGCAT CCTGCACAGC GCCCGCGCGC TCTTCGTGGG GCTGAGAGAC
    GGCGTCCTGC GGGTCCCACT GGAGAGGTGC GCCGCCTACC GCAGCCAGGG GGCATGCCTG
    GGGGCCCGGG ACCCGTACTG TGGCTGGGAC GGGAAGCAGC AACGTTGCAG CACACTCGAG
    GACAGCTCCA ACATGAGCCT CTGGACCCAG AACATCACCG CCTGTCCTGT GCGGAATGTG
    ACACGGGATG GGGGCTTCGG CCCATGGTCA CCATGGCAAC CATGTGAGCA CTTGGATGGG
    GACAACTCAG GCTCTTGCCT GTGTCGAGCT CGATCCTGTG ATTCCCCTCG ACCCCGCTGT
    GGGGGCCTTG ACTGCCTGGG GCCAGCCATC CACATCGCCA ACTGCTCCAG GAATGGGGCG
    TGGACCCCGT GGTCATCGTG GGCGCTGTGC AGCACGTCCT GTGGCATCGG CTTCCAGGTC
    CGCCAGCGAA GTTGCAGCAA CCCTGCTCCC CGCCACGGGG GCCGCATCTG CGTGGGCAAG
    AGCCGGGAGG AACGGTTCTG TAATGAGAAC ACGCCTTGCC CGGTGCCCAT CTTCTGGGCT
    TCCTGGGGCT CCTGGAGCAA GTGCAGCAGC AACTGTGGAG GGGGCATGCA GTCGCGGCGT
    CGGGCCTGCG AGAACGGCAA CTCCTGCCTG GGCTGCGGCG TGGAGTTCAA GACGTGCAAC
    CCCGAGGGCT GCCCCGAAGT GCGGCGCAAC ACCCCCTGGA CGCCGTGGCT GCCCGTGAAC
    GTGACGCAGG GCGGGGCACG GCAGGAGCAG CGGTTCCGCT TCACCTGCCG CGCGCCCCTT
    GCAGACCCGC ACGGCCTGCA GTTCGGCAGG AGAAGGACCG AGACGAGGAC CTGTCCCGCG
    GACGGCTCCG GCTCCTGCGA CACCGACGCC CTGGTGGAGG TCCTCCTGCG CAGCGGGAGC
    ACCTCCCCGC ACACGGTGAG CGGGGGCTGG GCCGCCTGGG GCCCGTGGTC GTCCTGCTCC
    CGGGACTGCG AGCTGGGCTT CCGCGTCCGC AAGAGAACGT GCACTAACCC GGAGCCCCGC
    AACGGGGGCC TGCCCTGCGT GGGCGATGCT GCCGAGTACC AGGACTGCAA CCCCCAGGCT
    TGCCCAGTTC GGGGTGCTTG GTCCTGCTGG ACCTCATGGT CTCCATGCTC AGCTTCCTGT
    GGTGGGGGTC ACTATCAACG CACCCGTTCC TGCACCAGCC CCGCACCCTC CCCAGGTGAG
    GACATCTGTC TCGGGCTGCA CACGGAGGAG GCACTATGTG CCACACAGGC CTGCCCAGAA
    GGCTGGTCGC CCTGGTCTGA GTGGAGTAAG TGCACTGACG ACGGAGCCCA GAGCCGAAGC
    CGGCACTGTG AGGAGCTCCT CCCAGGGTCC AGCGCCTGTG CTGGAAACAG CAGCCAGAGC
    CGCCCCTGCC CCTACAGCGA GATTCCCGTC ATCCTGCCAG CCTCCAGCAT GGAGGAGGCC
    ACCGACTGTG CAGGGTTCAA TCTCATCCAC TTGGTGGCCA CGGGCATCTC CTGCTTCTTG
    GGCTCTGGGC TCCTGACCCT AGCAGTGTAC CTGTCTTGCC AGCACTGCCA GCGTCAGTCC
    CAGGAGTCCA CACTGGTCCA TCCTGCCACC CCCAACCATT TGCACTACAA GGGCGGAGGC
    ACCCCGAAGA ATGAAAAGTA CACACCCATG GAATTCAAGA CCCTGAACAA GAATAACTTG
    ATCCCTGATG ACAGAGCCAA CTTCTACCCA TTGCAGCAGA CCAATGTGTA CACGACTACT
    TACTACCCAA GCCCCCTGAA CAAACACAGC TTCCGGCCCG AGGCCTCACC TGGACAACGG
    TGCTTCCCCA ACAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94293
    Clone ID Related Accession (Same CDS sequence) XM_017006639.1
    Accession Version XM_017006639.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product semaphorin-5B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
    TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC
    GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC
    ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC
    ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG
    GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT
    GAGGAGGAGT GTCAGAACTA CGTGCGAGTC CTGATCGTCG CCGGCCGGAA GGTGTTCATG
    TGTGGAACCA ATGCCTTTTC CCCCATGTGC ACCAGCAGAC AGGTGGGGAA CCTCAGCCGG
    ACTATTGAGA AGATCAATGG TGTGGCCCGC TGCCCCTATG ACCCACGCCA CAACTCCACA
    GCTGTCATCT CCTCCCAGGG GGAGCTCTAT GCAGCCACGG TCATCGACTT CTCAGGTCGG
    GACCCTGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAATATAAC
    TCCAAGTGGC TTAATGAGCC AAACTTCGTG GCAGCCTATG ATATTGGGCT GTTTGCATAC
    TTCTTCCTGC GGGAGAACGC AGTGGAGCAC GACTGTGGAC GCACCGTGTA CTCTCGCGTG
    GCCCGCGTGT GCAAGAATGA CGTGGGGGGC CGATTCCTGC TGGAGGACAC ATGGACCACA
    TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGCGAGG TCCCCTTCTA CTATAACGAG
    CTGCAGAGTG CCTTCCACTT GCCGGAGCAG GACCTCATCT ATGGAGTTTT CACAACCAAC
    GTAAACAGCA TCGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCTAT CTCCCAGGCT
    TTCAATGGCC CATTTCGCTA CCAGGAGAAC CCCAGGGCTG CCTGGCTCCC CATAGCCAAC
    CCCATCCCCA ATTTCCAGTG TGGCACCCTG CCTGAGACCG GTCCCAACGA GAACCTGACG
    GAGCGCAGCC TGCAGGACGC GCAGCGCCTC TTCCTGATGA GCGAGGCCGT GCAGCCGGTG
    ACACCCGAGC CCTGTGTCAC CCAGGACAGC GTGCGCTTCT CACACCTCGT GGTGGACCTG
    GTGCAGGCTA AAGACACGCT CTACCATGTA CTCTACATTG GCACCGAGTC GGGCACCATC
    CTGAAGGCGC TGTCCACGGC GAGCCGCAGC CTCCACGGCT GCTACCTGGA GGAGCTGCAC
    GTGCTGCCCC CCGGGCGCCG CGAGCCCCTG CGCAGCCTGC GCATCCTGCA CAGCGCCCGC
    GCGCTCTTCG TGGGGCTGAG AGACGGCGTC CTGCGGGTCC CACTGGAGAG GTGCGCCGCC
    TACCGCAGCC AGGGGGCATG CCTGGGGGCC CGGGACCCGT ACTGTGGCTG GGACGGGAAG
    CAGCAACGTT GCAGCACACT CGAGGACAGC TCCAACATGA GCCTCTGGAC CCAGAACATC
    ACCGCCTGTC CTGTGCGGAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG
    CAACCATGTG AGCACTTGGA TGGGGACAAC TCAGGCTCTT GCCTGTGTCG AGCTCGATCC
    TGTGATTCCC CTCGACCCCG CTGTGGGGGC CTTGACTGCC TGGGGCCAGC CATCCACATC
    GCCAACTGCT CCAGGAATGG GGCGTGGACC CCGTGGTCAT CGTGGGCGCT GTGCAGCACG
    TCCTGTGGCA TCGGCTTCCA GGTCCGCCAG CGAAGTTGCA GCAACCCTGC TCCCCGCCAC
    GGGGGCCGCA TCTGCGTGGG CAAGAGCCGG GAGGAACGGT TCTGTAATGA GAACACGCCT
    TGCCCGGTGC CCATCTTCTG GGCTTCCTGG GGCTCCTGGA GCAAGTGCAG CAGCAACTGT
    GGAGGGGGCA TGCAGTCGCG GCGTCGGGCC TGCGAGAACG GCAACTCCTG CCTGGGCTGC
    GGCGTGGAGT TCAAGACGTG CAACCCCGAG GGCTGCCCCG AAGTGCGGCG CAACACCCCC
    TGGACGCCGT GGCTGCCCGT GAACGTGACG CAGGGCGGGG CACGGCAGGA GCAGCGGTTC
    CGCTTCACCT GCCGCGCGCC CCTTGCAGAC CCGCACGGCC TGCAGTTCGG CAGGAGAAGG
    ACCGAGACGA GGACCTGTCC CGCGGACGGC TCCGGCTCCT GCGACACCGA CGGTACCCCG
    CGCCCGGCGC CCCCTCCTTC CTCTCTCCCA GCCCCGGAGC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862128.1
    CDS354..2738
    Translation

    Target ORF information:

    RefSeq Version XM_017006639.1
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5B (SEMA5B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006639.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
    TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC
    GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC
    ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC
    ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG
    GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT
    GAGGAGGAGT GTCAGAACTA CGTGCGAGTC CTGATCGTCG CCGGCCGGAA GGTGTTCATG
    TGTGGAACCA ATGCCTTTTC CCCCATGTGC ACCAGCAGAC AGGTGGGGAA CCTCAGCCGG
    ACTATTGAGA AGATCAATGG TGTGGCCCGC TGCCCCTATG ACCCACGCCA CAACTCCACA
    GCTGTCATCT CCTCCCAGGG GGAGCTCTAT GCAGCCACGG TCATCGACTT CTCAGGTCGG
    GACCCTGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAATATAAC
    TCCAAGTGGC TTAATGAGCC AAACTTCGTG GCAGCCTATG ATATTGGGCT GTTTGCATAC
    TTCTTCCTGC GGGAGAACGC AGTGGAGCAC GACTGTGGAC GCACCGTGTA CTCTCGCGTG
    GCCCGCGTGT GCAAGAATGA CGTGGGGGGC CGATTCCTGC TGGAGGACAC ATGGACCACA
    TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGCGAGG TCCCCTTCTA CTATAACGAG
    CTGCAGAGTG CCTTCCACTT GCCGGAGCAG GACCTCATCT ATGGAGTTTT CACAACCAAC
    GTAAACAGCA TCGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCTAT CTCCCAGGCT
    TTCAATGGCC CATTTCGCTA CCAGGAGAAC CCCAGGGCTG CCTGGCTCCC CATAGCCAAC
    CCCATCCCCA ATTTCCAGTG TGGCACCCTG CCTGAGACCG GTCCCAACGA GAACCTGACG
    GAGCGCAGCC TGCAGGACGC GCAGCGCCTC TTCCTGATGA GCGAGGCCGT GCAGCCGGTG
    ACACCCGAGC CCTGTGTCAC CCAGGACAGC GTGCGCTTCT CACACCTCGT GGTGGACCTG
    GTGCAGGCTA AAGACACGCT CTACCATGTA CTCTACATTG GCACCGAGTC GGGCACCATC
    CTGAAGGCGC TGTCCACGGC GAGCCGCAGC CTCCACGGCT GCTACCTGGA GGAGCTGCAC
    GTGCTGCCCC CCGGGCGCCG CGAGCCCCTG CGCAGCCTGC GCATCCTGCA CAGCGCCCGC
    GCGCTCTTCG TGGGGCTGAG AGACGGCGTC CTGCGGGTCC CACTGGAGAG GTGCGCCGCC
    TACCGCAGCC AGGGGGCATG CCTGGGGGCC CGGGACCCGT ACTGTGGCTG GGACGGGAAG
    CAGCAACGTT GCAGCACACT CGAGGACAGC TCCAACATGA GCCTCTGGAC CCAGAACATC
    ACCGCCTGTC CTGTGCGGAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG
    CAACCATGTG AGCACTTGGA TGGGGACAAC TCAGGCTCTT GCCTGTGTCG AGCTCGATCC
    TGTGATTCCC CTCGACCCCG CTGTGGGGGC CTTGACTGCC TGGGGCCAGC CATCCACATC
    GCCAACTGCT CCAGGAATGG GGCGTGGACC CCGTGGTCAT CGTGGGCGCT GTGCAGCACG
    TCCTGTGGCA TCGGCTTCCA GGTCCGCCAG CGAAGTTGCA GCAACCCTGC TCCCCGCCAC
    GGGGGCCGCA TCTGCGTGGG CAAGAGCCGG GAGGAACGGT TCTGTAATGA GAACACGCCT
    TGCCCGGTGC CCATCTTCTG GGCTTCCTGG GGCTCCTGGA GCAAGTGCAG CAGCAACTGT
    GGAGGGGGCA TGCAGTCGCG GCGTCGGGCC TGCGAGAACG GCAACTCCTG CCTGGGCTGC
    GGCGTGGAGT TCAAGACGTG CAACCCCGAG GGCTGCCCCG AAGTGCGGCG CAACACCCCC
    TGGACGCCGT GGCTGCCCGT GAACGTGACG CAGGGCGGGG CACGGCAGGA GCAGCGGTTC
    CGCTTCACCT GCCGCGCGCC CCTTGCAGAC CCGCACGGCC TGCAGTTCGG CAGGAGAAGG
    ACCGAGACGA GGACCTGTCC CGCGGACGGC TCCGGCTCCT GCGACACCGA CGGTACCCCG
    CGCCCGGCGC CCCCTCCTTC CTCTCTCCCA GCCCCGGAGC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94292
    Clone ID Related Accession (Same CDS sequence) XM_017006638.2 , XM_017006638.1
    Accession Version XM_017006638.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product semaphorin-5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017006638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
    TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC
    GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC
    ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC
    ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG
    GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT
    GAGTGGTGTG AGCGTGAGAT ATCCAGCATC GCACCTGGAG AGCTGTGCTG TCTGCTCCTT
    TCCTTCCTGC CTCAGGAGGA GTGTCAGAAC TACGTGCGAG TCCTGATCGT CGCCGGCCGG
    AAGGTGTTCA TGTGTGGAAC CAATGCCTTT TCCCCCATGT GCACCAGCAG ACAGGTGGGG
    AACCTCAGCC GGACTATTGA GAAGATCAAT GGTGTGGCCC GCTGCCCCTA TGACCCACGC
    CACAACTCCA CAGCTGTCAT CTCCTCCCAG GGGGAGCTCT ATGCAGCCAC GGTCATCGAC
    TTCTCAGGTC GGGACCCTGC CATCTACCGC AGCCTGGGCA GTGGGCCACC GCTTCGCACT
    GCCCAATATA ACTCCAAGTG GCTTAATGAG CCAAACTTCG TGGCAGCCTA TGATATTGGG
    CTGTTTGCAT ACTTCTTCCT GCGGGAGAAC GCAGTGGAGC ACGACTGTGG ACGCACCGTG
    TACTCTCGCG TGGCCCGCGT GTGCAAGAAT GACGTGGGGG GCCGATTCCT GCTGGAGGAC
    ACATGGACCA CATTCATGAA GGCCCGGCTC AACTGCTCCC GCCCGGGCGA GGTCCCCTTC
    TACTATAACG AGCTGCAGAG TGCCTTCCAC TTGCCGGAGC AGGACCTCAT CTATGGAGTT
    TTCACAACCA ACGTAAACAG CATCGCGGCT TCTGCTGTCT GCGCCTTCAA CCTCAGTGCT
    ATCTCCCAGG CTTTCAATGG CCCATTTCGC TACCAGGAGA ACCCCAGGGC TGCCTGGCTC
    CCCATAGCCA ACCCCATCCC CAATTTCCAG TGTGGCACCC TGCCTGAGAC CGGTCCCAAC
    GAGAACCTGA CGGAGCGCAG CCTGCAGGAC GCGCAGCGCC TCTTCCTGAT GAGCGAGGCC
    GTGCAGCCGG TGACACCCGA GCCCTGTGTC ACCCAGGACA GCGTGCGCTT CTCACACCTC
    GTGGTGGACC TGGTGCAGGC TAAAGACACG CTCTACCATG TACTCTACAT TGGCACCGAG
    TCGGGCACCA TCCTGAAGGC GCTGTCCACG GCGAGCCGCA GCCTCCACGG CTGCTACCTG
    GAGGAGCTGC ACGTGCTGCC CCCCGGGCGC CGCGAGCCCC TGCGCAGCCT GCGCATCCTG
    CACAGCGCCC GCGCGCTCTT CGTGGGGCTG AGAGACGGCG TCCTGCGGGT CCCACTGGAG
    AGGTGCGCCG CCTACCGCAG CCAGGGGGCA TGCCTGGGGG CCCGGGACCC GTACTGTGGC
    TGGGACGGGA AGCAGCAACG TTGCAGCACA CTCGAGGACA GCTCCAACAT GAGCCTCTGG
    ACCCAGAACA TCACCGCCTG TCCTGTGCGG AATGTGACAC GGGATGGGGG CTTCGGCCCA
    TGGTCACCAT GGCAACCATG TGAGCACTTG GATGGGGACA ACTCAGGCTC TTGCCTGTGT
    CGAGCTCGAT CCTGTGATTC CCCTCGACCC CGCTGTGGGG GCCTTGACTG CCTGGGGCCA
    GCCATCCACA TCGCCAACTG CTCCAGGAAT GGGGCGTGGA CCCCGTGGTC ATCGTGGGCG
    CTGTGCAGCA CGTCCTGTGG CATCGGCTTC CAGGTCCGCC AGCGAAGTTG CAGCAACCCT
    GCTCCCCGCC ACGGGGGCCG CATCTGCGTG GGCAAGAGCC GGGAGGAACG GTTCTGTAAT
    GAGAACACGC CTTGCCCGGT GCCCATCTTC TGGGCTTCCT GGGGCTCCTG GAGCAAGTGC
    AGCAGCAACT GTGGAGGGGG CATGCAGTCG CGGCGTCGGG CCTGCGAGAA CGGCAACTCC
    TGCCTGGGCT GCGGCGTGGA GTTCAAGACG TGCAACCCCG AGGGCTGCCC CGAAGTGCGG
    CGCAACACCC CCTGGACGCC GTGGCTGCCC GTGAACGTGA CGCAGGGCGG GGCACGGCAG
    GAGCAGCGGT TCCGCTTCAC CTGCCGCGCG CCCCTTGCAG ACCCGCACGG CCTGCAGTTC
    GGCAGGAGAA GGACCGAGAC GAGGACCTGT CCCGCGGACG GCTCCGGCTC CTGCGACACC
    GACGCCCTGG TGGAGGTCCT CCTGCGCAGC GGGAGCACCT CCCCGCACAC GGTGAGCGGG
    GGCTGGGCCG CCTGGGGCCC GTGGTCGTCC TGCTCCCGGG ACTGCGAGCT GGGCTTCCGC
    GTCCGCAAGA GAACGTGCAC TAACCCGGAG CCCCGCAACG GGGGCCTGCC CTGCGTGGGC
    GATGCTGCCG AGTACCAGGA CTGCAACCCC CAGGCTTGCC CAGTTCGGGG TGCTTGGTCC
    TGCTGGACCT CATGGTCTCC ATGCTCAGCT TCCTGTGGTG GGGGTCACTA TCAACGCACC
    CGTTCCTGCA CCAGCCCCGC ACCCTCCCCA GGTGAGGACA TCTGTCTCGG GCTGCACACG
    GAGGAGGCAC TATGTGCCAC ACAGGCCTGC CCAGAAGGCT GGTCGCCCTG GTCTGAGTGG
    AGTAAGTGCA CTGACGACGG AGCCCAGAGC CGAAGCCGGC ACTGTGAGGA GCTCCTCCCA
    GGGTCCAGCG CCTGTGCTGG AAACAGCAGC CAGAGCCGCC CCTGCCCCTA CAGCGAGATT
    CCCGTCATCC TGCCAGCCTC CAGCATGGAG GAGGCCACCG ACTGTGCAGG GTTCAATCTC
    ATCCACTTGG TGGCCACGGG CATCTCCTGC TTCTTGGGCT CTGGGCTCCT GACCCTAGCA
    GTGTACCTGT CTTGCCAGCA CTGCCAGCGT CAGTCCCAGG AGTCCACACT GGTCCATCCT
    GCCACCCCCA ACCATTTGCA CTACAAGGGC GGAGGCACCC CGAAGAATGA AAAGTACACA
    CCCATGGAAT TCAAGACCCT GAACAAGAAT AACTTGATCC CTGATGACAG AGCCAACTTC
    TACCCATTGC AGCAGACCAA TGTGTACACG ACTACTTACT ACCCAAGCCC CCTGAACAAA
    CACAGCTTCC GGCCCGAGGC CTCACCTGGA CAACGGTGCT TCCCCAACAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862127.1
    CDS1156..4509
    Translation

    Target ORF information:

    RefSeq Version XM_017006638.2
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5B (SEMA5B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006638.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
    TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC
    GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC
    ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC
    ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG
    GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT
    GAGTGGTGTG AGCGTGAGAT ATCCAGCATC GCACCTGGAG AGCTGTGCTG TCTGCTCCTT
    TCCTTCCTGC CTCAGGAGGA GTGTCAGAAC TACGTGCGAG TCCTGATCGT CGCCGGCCGG
    AAGGTGTTCA TGTGTGGAAC CAATGCCTTT TCCCCCATGT GCACCAGCAG ACAGGTGGGG
    AACCTCAGCC GGACTATTGA GAAGATCAAT GGTGTGGCCC GCTGCCCCTA TGACCCACGC
    CACAACTCCA CAGCTGTCAT CTCCTCCCAG GGGGAGCTCT ATGCAGCCAC GGTCATCGAC
    TTCTCAGGTC GGGACCCTGC CATCTACCGC AGCCTGGGCA GTGGGCCACC GCTTCGCACT
    GCCCAATATA ACTCCAAGTG GCTTAATGAG CCAAACTTCG TGGCAGCCTA TGATATTGGG
    CTGTTTGCAT ACTTCTTCCT GCGGGAGAAC GCAGTGGAGC ACGACTGTGG ACGCACCGTG
    TACTCTCGCG TGGCCCGCGT GTGCAAGAAT GACGTGGGGG GCCGATTCCT GCTGGAGGAC
    ACATGGACCA CATTCATGAA GGCCCGGCTC AACTGCTCCC GCCCGGGCGA GGTCCCCTTC
    TACTATAACG AGCTGCAGAG TGCCTTCCAC TTGCCGGAGC AGGACCTCAT CTATGGAGTT
    TTCACAACCA ACGTAAACAG CATCGCGGCT TCTGCTGTCT GCGCCTTCAA CCTCAGTGCT
    ATCTCCCAGG CTTTCAATGG CCCATTTCGC TACCAGGAGA ACCCCAGGGC TGCCTGGCTC
    CCCATAGCCA ACCCCATCCC CAATTTCCAG TGTGGCACCC TGCCTGAGAC CGGTCCCAAC
    GAGAACCTGA CGGAGCGCAG CCTGCAGGAC GCGCAGCGCC TCTTCCTGAT GAGCGAGGCC
    GTGCAGCCGG TGACACCCGA GCCCTGTGTC ACCCAGGACA GCGTGCGCTT CTCACACCTC
    GTGGTGGACC TGGTGCAGGC TAAAGACACG CTCTACCATG TACTCTACAT TGGCACCGAG
    TCGGGCACCA TCCTGAAGGC GCTGTCCACG GCGAGCCGCA GCCTCCACGG CTGCTACCTG
    GAGGAGCTGC ACGTGCTGCC CCCCGGGCGC CGCGAGCCCC TGCGCAGCCT GCGCATCCTG
    CACAGCGCCC GCGCGCTCTT CGTGGGGCTG AGAGACGGCG TCCTGCGGGT CCCACTGGAG
    AGGTGCGCCG CCTACCGCAG CCAGGGGGCA TGCCTGGGGG CCCGGGACCC GTACTGTGGC
    TGGGACGGGA AGCAGCAACG TTGCAGCACA CTCGAGGACA GCTCCAACAT GAGCCTCTGG
    ACCCAGAACA TCACCGCCTG TCCTGTGCGG AATGTGACAC GGGATGGGGG CTTCGGCCCA
    TGGTCACCAT GGCAACCATG TGAGCACTTG GATGGGGACA ACTCAGGCTC TTGCCTGTGT
    CGAGCTCGAT CCTGTGATTC CCCTCGACCC CGCTGTGGGG GCCTTGACTG CCTGGGGCCA
    GCCATCCACA TCGCCAACTG CTCCAGGAAT GGGGCGTGGA CCCCGTGGTC ATCGTGGGCG
    CTGTGCAGCA CGTCCTGTGG CATCGGCTTC CAGGTCCGCC AGCGAAGTTG CAGCAACCCT
    GCTCCCCGCC ACGGGGGCCG CATCTGCGTG GGCAAGAGCC GGGAGGAACG GTTCTGTAAT
    GAGAACACGC CTTGCCCGGT GCCCATCTTC TGGGCTTCCT GGGGCTCCTG GAGCAAGTGC
    AGCAGCAACT GTGGAGGGGG CATGCAGTCG CGGCGTCGGG CCTGCGAGAA CGGCAACTCC
    TGCCTGGGCT GCGGCGTGGA GTTCAAGACG TGCAACCCCG AGGGCTGCCC CGAAGTGCGG
    CGCAACACCC CCTGGACGCC GTGGCTGCCC GTGAACGTGA CGCAGGGCGG GGCACGGCAG
    GAGCAGCGGT TCCGCTTCAC CTGCCGCGCG CCCCTTGCAG ACCCGCACGG CCTGCAGTTC
    GGCAGGAGAA GGACCGAGAC GAGGACCTGT CCCGCGGACG GCTCCGGCTC CTGCGACACC
    GACGCCCTGG TGGAGGTCCT CCTGCGCAGC GGGAGCACCT CCCCGCACAC GGTGAGCGGG
    GGCTGGGCCG CCTGGGGCCC GTGGTCGTCC TGCTCCCGGG ACTGCGAGCT GGGCTTCCGC
    GTCCGCAAGA GAACGTGCAC TAACCCGGAG CCCCGCAACG GGGGCCTGCC CTGCGTGGGC
    GATGCTGCCG AGTACCAGGA CTGCAACCCC CAGGCTTGCC CAGTTCGGGG TGCTTGGTCC
    TGCTGGACCT CATGGTCTCC ATGCTCAGCT TCCTGTGGTG GGGGTCACTA TCAACGCACC
    CGTTCCTGCA CCAGCCCCGC ACCCTCCCCA GGTGAGGACA TCTGTCTCGG GCTGCACACG
    GAGGAGGCAC TATGTGCCAC ACAGGCCTGC CCAGAAGGCT GGTCGCCCTG GTCTGAGTGG
    AGTAAGTGCA CTGACGACGG AGCCCAGAGC CGAAGCCGGC ACTGTGAGGA GCTCCTCCCA
    GGGTCCAGCG CCTGTGCTGG AAACAGCAGC CAGAGCCGCC CCTGCCCCTA CAGCGAGATT
    CCCGTCATCC TGCCAGCCTC CAGCATGGAG GAGGCCACCG ACTGTGCAGG GTTCAATCTC
    ATCCACTTGG TGGCCACGGG CATCTCCTGC TTCTTGGGCT CTGGGCTCCT GACCCTAGCA
    GTGTACCTGT CTTGCCAGCA CTGCCAGCGT CAGTCCCAGG AGTCCACACT GGTCCATCCT
    GCCACCCCCA ACCATTTGCA CTACAAGGGC GGAGGCACCC CGAAGAATGA AAAGTACACA
    CCCATGGAAT TCAAGACCCT GAACAAGAAT AACTTGATCC CTGATGACAG AGCCAACTTC
    TACCCATTGC AGCAGACCAA TGTGTACACG ACTACTTACT ACCCAAGCCC CCTGAACAAA
    CACAGCTTCC GGCCCGAGGC CTCACCTGGA CAACGGTGCT TCCCCAACAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94292
    Clone ID Related Accession (Same CDS sequence) XM_017006638.2 , XM_017006638.1
    Accession Version XM_017006638.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3354bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product semaphorin-5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
    TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC
    GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC
    ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC
    ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG
    GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT
    GAGTGGTGTG AGCGTGAGAT ATCCAGCATC GCACCTGGAG AGCTGTGCTG TCTGCTCCTT
    TCCTTCCTGC CTCAGGAGGA GTGTCAGAAC TACGTGCGAG TCCTGATCGT CGCCGGCCGG
    AAGGTGTTCA TGTGTGGAAC CAATGCCTTT TCCCCCATGT GCACCAGCAG ACAGGTGGGG
    AACCTCAGCC GGACTATTGA GAAGATCAAT GGTGTGGCCC GCTGCCCCTA TGACCCACGC
    CACAACTCCA CAGCTGTCAT CTCCTCCCAG GGGGAGCTCT ATGCAGCCAC GGTCATCGAC
    TTCTCAGGTC GGGACCCTGC CATCTACCGC AGCCTGGGCA GTGGGCCACC GCTTCGCACT
    GCCCAATATA ACTCCAAGTG GCTTAATGAG CCAAACTTCG TGGCAGCCTA TGATATTGGG
    CTGTTTGCAT ACTTCTTCCT GCGGGAGAAC GCAGTGGAGC ACGACTGTGG ACGCACCGTG
    TACTCTCGCG TGGCCCGCGT GTGCAAGAAT GACGTGGGGG GCCGATTCCT GCTGGAGGAC
    ACATGGACCA CATTCATGAA GGCCCGGCTC AACTGCTCCC GCCCGGGCGA GGTCCCCTTC
    TACTATAACG AGCTGCAGAG TGCCTTCCAC TTGCCGGAGC AGGACCTCAT CTATGGAGTT
    TTCACAACCA ACGTAAACAG CATCGCGGCT TCTGCTGTCT GCGCCTTCAA CCTCAGTGCT
    ATCTCCCAGG CTTTCAATGG CCCATTTCGC TACCAGGAGA ACCCCAGGGC TGCCTGGCTC
    CCCATAGCCA ACCCCATCCC CAATTTCCAG TGTGGCACCC TGCCTGAGAC CGGTCCCAAC
    GAGAACCTGA CGGAGCGCAG CCTGCAGGAC GCGCAGCGCC TCTTCCTGAT GAGCGAGGCC
    GTGCAGCCGG TGACACCCGA GCCCTGTGTC ACCCAGGACA GCGTGCGCTT CTCACACCTC
    GTGGTGGACC TGGTGCAGGC TAAAGACACG CTCTACCATG TACTCTACAT TGGCACCGAG
    TCGGGCACCA TCCTGAAGGC GCTGTCCACG GCGAGCCGCA GCCTCCACGG CTGCTACCTG
    GAGGAGCTGC ACGTGCTGCC CCCCGGGCGC CGCGAGCCCC TGCGCAGCCT GCGCATCCTG
    CACAGCGCCC GCGCGCTCTT CGTGGGGCTG AGAGACGGCG TCCTGCGGGT CCCACTGGAG
    AGGTGCGCCG CCTACCGCAG CCAGGGGGCA TGCCTGGGGG CCCGGGACCC GTACTGTGGC
    TGGGACGGGA AGCAGCAACG TTGCAGCACA CTCGAGGACA GCTCCAACAT GAGCCTCTGG
    ACCCAGAACA TCACCGCCTG TCCTGTGCGG AATGTGACAC GGGATGGGGG CTTCGGCCCA
    TGGTCACCAT GGCAACCATG TGAGCACTTG GATGGGGACA ACTCAGGCTC TTGCCTGTGT
    CGAGCTCGAT CCTGTGATTC CCCTCGACCC CGCTGTGGGG GCCTTGACTG CCTGGGGCCA
    GCCATCCACA TCGCCAACTG CTCCAGGAAT GGGGCGTGGA CCCCGTGGTC ATCGTGGGCG
    CTGTGCAGCA CGTCCTGTGG CATCGGCTTC CAGGTCCGCC AGCGAAGTTG CAGCAACCCT
    GCTCCCCGCC ACGGGGGCCG CATCTGCGTG GGCAAGAGCC GGGAGGAACG GTTCTGTAAT
    GAGAACACGC CTTGCCCGGT GCCCATCTTC TGGGCTTCCT GGGGCTCCTG GAGCAAGTGC
    AGCAGCAACT GTGGAGGGGG CATGCAGTCG CGGCGTCGGG CCTGCGAGAA CGGCAACTCC
    TGCCTGGGCT GCGGCGTGGA GTTCAAGACG TGCAACCCCG AGGGCTGCCC CGAAGTGCGG
    CGCAACACCC CCTGGACGCC GTGGCTGCCC GTGAACGTGA CGCAGGGCGG GGCACGGCAG
    GAGCAGCGGT TCCGCTTCAC CTGCCGCGCG CCCCTTGCAG ACCCGCACGG CCTGCAGTTC
    GGCAGGAGAA GGACCGAGAC GAGGACCTGT CCCGCGGACG GCTCCGGCTC CTGCGACACC
    GACGCCCTGG TGGAGGTCCT CCTGCGCAGC GGGAGCACCT CCCCGCACAC GGTGAGCGGG
    GGCTGGGCCG CCTGGGGCCC GTGGTCGTCC TGCTCCCGGG ACTGCGAGCT GGGCTTCCGC
    GTCCGCAAGA GAACGTGCAC TAACCCGGAG CCCCGCAACG GGGGCCTGCC CTGCGTGGGC
    GATGCTGCCG AGTACCAGGA CTGCAACCCC CAGGCTTGCC CAGTTCGGGG TGCTTGGTCC
    TGCTGGACCT CATGGTCTCC ATGCTCAGCT TCCTGTGGTG GGGGTCACTA TCAACGCACC
    CGTTCCTGCA CCAGCCCCGC ACCCTCCCCA GGTGAGGACA TCTGTCTCGG GCTGCACACG
    GAGGAGGCAC TATGTGCCAC ACAGGCCTGC CCAGAAGGCT GGTCGCCCTG GTCTGAGTGG
    AGTAAGTGCA CTGACGACGG AGCCCAGAGC CGAAGCCGGC ACTGTGAGGA GCTCCTCCCA
    GGGTCCAGCG CCTGTGCTGG AAACAGCAGC CAGAGCCGCC CCTGCCCCTA CAGCGAGATT
    CCCGTCATCC TGCCAGCCTC CAGCATGGAG GAGGCCACCG ACTGTGCAGG GTTCAATCTC
    ATCCACTTGG TGGCCACGGG CATCTCCTGC TTCTTGGGCT CTGGGCTCCT GACCCTAGCA
    GTGTACCTGT CTTGCCAGCA CTGCCAGCGT CAGTCCCAGG AGTCCACACT GGTCCATCCT
    GCCACCCCCA ACCATTTGCA CTACAAGGGC GGAGGCACCC CGAAGAATGA AAAGTACACA
    CCCATGGAAT TCAAGACCCT GAACAAGAAT AACTTGATCC CTGATGACAG AGCCAACTTC
    TACCCATTGC AGCAGACCAA TGTGTACACG ACTACTTACT ACCCAAGCCC CCTGAACAAA
    CACAGCTTCC GGCCCGAGGC CTCACCTGGA CAACGGTGCT TCCCCAACAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862127.1
    CDS540..3893
    Translation

    Target ORF information:

    RefSeq Version XM_017006638.1
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5B (SEMA5B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017006638.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
    TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC
    GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC
    ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC
    ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG
    GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT
    GAGTGGTGTG AGCGTGAGAT ATCCAGCATC GCACCTGGAG AGCTGTGCTG TCTGCTCCTT
    TCCTTCCTGC CTCAGGAGGA GTGTCAGAAC TACGTGCGAG TCCTGATCGT CGCCGGCCGG
    AAGGTGTTCA TGTGTGGAAC CAATGCCTTT TCCCCCATGT GCACCAGCAG ACAGGTGGGG
    AACCTCAGCC GGACTATTGA GAAGATCAAT GGTGTGGCCC GCTGCCCCTA TGACCCACGC
    CACAACTCCA CAGCTGTCAT CTCCTCCCAG GGGGAGCTCT ATGCAGCCAC GGTCATCGAC
    TTCTCAGGTC GGGACCCTGC CATCTACCGC AGCCTGGGCA GTGGGCCACC GCTTCGCACT
    GCCCAATATA ACTCCAAGTG GCTTAATGAG CCAAACTTCG TGGCAGCCTA TGATATTGGG
    CTGTTTGCAT ACTTCTTCCT GCGGGAGAAC GCAGTGGAGC ACGACTGTGG ACGCACCGTG
    TACTCTCGCG TGGCCCGCGT GTGCAAGAAT GACGTGGGGG GCCGATTCCT GCTGGAGGAC
    ACATGGACCA CATTCATGAA GGCCCGGCTC AACTGCTCCC GCCCGGGCGA GGTCCCCTTC
    TACTATAACG AGCTGCAGAG TGCCTTCCAC TTGCCGGAGC AGGACCTCAT CTATGGAGTT
    TTCACAACCA ACGTAAACAG CATCGCGGCT TCTGCTGTCT GCGCCTTCAA CCTCAGTGCT
    ATCTCCCAGG CTTTCAATGG CCCATTTCGC TACCAGGAGA ACCCCAGGGC TGCCTGGCTC
    CCCATAGCCA ACCCCATCCC CAATTTCCAG TGTGGCACCC TGCCTGAGAC CGGTCCCAAC
    GAGAACCTGA CGGAGCGCAG CCTGCAGGAC GCGCAGCGCC TCTTCCTGAT GAGCGAGGCC
    GTGCAGCCGG TGACACCCGA GCCCTGTGTC ACCCAGGACA GCGTGCGCTT CTCACACCTC
    GTGGTGGACC TGGTGCAGGC TAAAGACACG CTCTACCATG TACTCTACAT TGGCACCGAG
    TCGGGCACCA TCCTGAAGGC GCTGTCCACG GCGAGCCGCA GCCTCCACGG CTGCTACCTG
    GAGGAGCTGC ACGTGCTGCC CCCCGGGCGC CGCGAGCCCC TGCGCAGCCT GCGCATCCTG
    CACAGCGCCC GCGCGCTCTT CGTGGGGCTG AGAGACGGCG TCCTGCGGGT CCCACTGGAG
    AGGTGCGCCG CCTACCGCAG CCAGGGGGCA TGCCTGGGGG CCCGGGACCC GTACTGTGGC
    TGGGACGGGA AGCAGCAACG TTGCAGCACA CTCGAGGACA GCTCCAACAT GAGCCTCTGG
    ACCCAGAACA TCACCGCCTG TCCTGTGCGG AATGTGACAC GGGATGGGGG CTTCGGCCCA
    TGGTCACCAT GGCAACCATG TGAGCACTTG GATGGGGACA ACTCAGGCTC TTGCCTGTGT
    CGAGCTCGAT CCTGTGATTC CCCTCGACCC CGCTGTGGGG GCCTTGACTG CCTGGGGCCA
    GCCATCCACA TCGCCAACTG CTCCAGGAAT GGGGCGTGGA CCCCGTGGTC ATCGTGGGCG
    CTGTGCAGCA CGTCCTGTGG CATCGGCTTC CAGGTCCGCC AGCGAAGTTG CAGCAACCCT
    GCTCCCCGCC ACGGGGGCCG CATCTGCGTG GGCAAGAGCC GGGAGGAACG GTTCTGTAAT
    GAGAACACGC CTTGCCCGGT GCCCATCTTC TGGGCTTCCT GGGGCTCCTG GAGCAAGTGC
    AGCAGCAACT GTGGAGGGGG CATGCAGTCG CGGCGTCGGG CCTGCGAGAA CGGCAACTCC
    TGCCTGGGCT GCGGCGTGGA GTTCAAGACG TGCAACCCCG AGGGCTGCCC CGAAGTGCGG
    CGCAACACCC CCTGGACGCC GTGGCTGCCC GTGAACGTGA CGCAGGGCGG GGCACGGCAG
    GAGCAGCGGT TCCGCTTCAC CTGCCGCGCG CCCCTTGCAG ACCCGCACGG CCTGCAGTTC
    GGCAGGAGAA GGACCGAGAC GAGGACCTGT CCCGCGGACG GCTCCGGCTC CTGCGACACC
    GACGCCCTGG TGGAGGTCCT CCTGCGCAGC GGGAGCACCT CCCCGCACAC GGTGAGCGGG
    GGCTGGGCCG CCTGGGGCCC GTGGTCGTCC TGCTCCCGGG ACTGCGAGCT GGGCTTCCGC
    GTCCGCAAGA GAACGTGCAC TAACCCGGAG CCCCGCAACG GGGGCCTGCC CTGCGTGGGC
    GATGCTGCCG AGTACCAGGA CTGCAACCCC CAGGCTTGCC CAGTTCGGGG TGCTTGGTCC
    TGCTGGACCT CATGGTCTCC ATGCTCAGCT TCCTGTGGTG GGGGTCACTA TCAACGCACC
    CGTTCCTGCA CCAGCCCCGC ACCCTCCCCA GGTGAGGACA TCTGTCTCGG GCTGCACACG
    GAGGAGGCAC TATGTGCCAC ACAGGCCTGC CCAGAAGGCT GGTCGCCCTG GTCTGAGTGG
    AGTAAGTGCA CTGACGACGG AGCCCAGAGC CGAAGCCGGC ACTGTGAGGA GCTCCTCCCA
    GGGTCCAGCG CCTGTGCTGG AAACAGCAGC CAGAGCCGCC CCTGCCCCTA CAGCGAGATT
    CCCGTCATCC TGCCAGCCTC CAGCATGGAG GAGGCCACCG ACTGTGCAGG GTTCAATCTC
    ATCCACTTGG TGGCCACGGG CATCTCCTGC TTCTTGGGCT CTGGGCTCCT GACCCTAGCA
    GTGTACCTGT CTTGCCAGCA CTGCCAGCGT CAGTCCCAGG AGTCCACACT GGTCCATCCT
    GCCACCCCCA ACCATTTGCA CTACAAGGGC GGAGGCACCC CGAAGAATGA AAAGTACACA
    CCCATGGAAT TCAAGACCCT GAACAAGAAT AACTTGATCC CTGATGACAG AGCCAACTTC
    TACCCATTGC AGCAGACCAA TGTGTACACG ACTACTTACT ACCCAAGCCC CCTGAACAAA
    CACAGCTTCC GGCCCGAGGC CTCACCTGGA CAACGGTGCT TCCCCAACAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00906
    Clone ID Related Accession (Same CDS sequence) NM_001256348.1
    Accession Version NM_001256348.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-23
    Organism Homo sapiens(human)
    Product semaphorin-5B isoform 3 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC083797.16, AK294528.1 and AC078794.13. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region, uses a downstream start codon and uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The resulting protein (isoform 3) is shorter and has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK294528.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
    TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC
    GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC
    ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC
    ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG
    GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT
    GAGGAGGAGT GTCAGAACTA CGTGCGAGTC CTGATCGTCG CCGGCCGGAA GGTGTTCATG
    TGTGGAACCA ATGCCTTTTC CCCCATGTGC ACCAGCAGAC AGGTGGGGAA CCTCAGCCGG
    ACTATTGAGA AGATCAATGG TGTGGCCCGC TGCCCCTATG ACCCACGCCA CAACTCCACA
    GCTGTCATCT CCTCCCAGGG GGAGCTCTAT GCAGCCACGG TCATCGACTT CTCAGGTCGG
    GACCCTGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAATATAAC
    TCCAAGTGGC TTAATGAGCC AAACTTCGTG GCAGCCTATG ATATTGGGCT GTTTGCATAC
    TTCTTCCTGC GGGAGAACGC AGTGGAGCAC GACTGTGGAC GCACCGTGTA CTCTCGCGTG
    GCCCGCGTGT GCAAGAATGA CGTGGGGGGC CGATTCCTGC TGGAGGACAC ATGGACCACA
    TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGCGAGG TCCCCTTCTA CTATAACGAG
    CTGCAGAGTG CCTTCCACTT GCCGGAGCAG GACCTCATCT ATGGAGTTTT CACAACCAAC
    GTAAACAGCA TCGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCTAT CTCCCAGGCT
    TTCAATGGCC CATTTCGCTA CCAGGAGAAC CCCAGGGCTG CCTGGCTCCC CATAGCCAAC
    CCCATCCCCA ATTTCCAGTG TGGCACCCTG CCTGAGACCG GTCCCAACGA GAACCTGACG
    GAGCGCAGCC TGCAGGACGC GCAGCGCCTC TTCCTGATGA GCGAGGCCGT GCAGCCGGTG
    ACACCCGAGC CCTGTGTCAC CCAGGACAGC GTGCGCTTCT CACACCTCGT GGTGGACCTG
    GTGCAGGCTA AAGACACGCT CTACCATGTA CTCTACATTG GCACCGAGTC GGGCACCATC
    CTGAAGGCGC TGTCCACGGC GAGCCGCAGC CTCCACGGCT GCTACCTGGA GGAGCTGCAC
    GTGCTGCCCC CCGGGCGCCG CGAGCCCCTG CGCAGCCTGC GCATCCTGCA CAGCGCCCGC
    GCGCTCTTCG TGGGGCTGAG AGACGGCGTC CTGCGGGTCC CACTGGAGAG GTGCGCCGCC
    TACCGCAGCC AGGGGGCATG CCTGGGGGCC CGGGACCCGT ACTGTGGCTG GGACGGGAAG
    CAGCAACGTT GCAGCACACT CGAGGACAGC TCCAACATGA GCCTCTGGAC CCAGAACATC
    ACCGCCTGTC CTGTGCGGAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG
    CAACCATGTG AGCACTTGGA TGGGGACAAC TCAGGCTCTT GCCTGTGTCG AGCTCGATCC
    TGTGATTCCC CTCGACCCCG CTGTGGGGGC CTTGACTGCC TGGGGCCAGC CATCCACATC
    GCCAACTGCT CCAGGAATGG GGCGTGGACC CCGTGGTCAT CGTGGGCGCT GTGCAGCACG
    TCCTGTGGCA TCGGCTTCCA GGTCCGCCAG CGAAGTTGCA GCAACCCTGC TCCCCGCCAC
    GGGGGCCGCA TCTGCGTGGG CAAGAGCCGG GAGGAACGGT TCTGTAATGA GAACACGCCT
    TGCCCGGTGC CCATCTTCTG GGCTTCCTGG GGCTCCTGGA GCAAGTGCAG CAGCAACTGT
    GGAGGGGGCA TGCAGTCGCG GCGTCGGGCC TGCGAGAACG GCAACTCCTG CCTGGGCTGC
    GGCGTGGAGT TCAAGACGTG CAACCCCGAG GGCTGCCCCG AAGTGCGGCG CAACACCCCC
    TGGACGCCGT GGCTGCCCGT GAACGTGACG CAGGGCGGGG CACGGCAGGA GCAGCGGTTC
    CGCTTCACCT GCCGCGCGCC CCTTGCAGAC CCGCACGGCC TGCAGTTCGG CAGGAGAAGG
    ACCGAGACGA GGACCTGTCC CGCGGACGGC TCCGGCTCCT GCGACACCGA CGCCCTGGTG
    GAGGTCCTCC TGCGCAGCGG GAGCACCTCC CCGCACACGG TGAGCGGGGG CTGGGCCGCC
    TGGGGCCCGT GGTCGTCCTG CTCCCGGGAC TGCGAGCTGG GCTTCCGCGT CCGCAAGAGA
    ACGTGCACTA ACCCGGAGCC CCGCAACGGG GGCCTGCCCT GCGTGGGCGA TGCTGCCGAG
    TACCAGGACT GCAACCCCCA GGCTTGCCCA GGTGAGGACA TCTGTCTCGG GCTGCACACG
    GAGGAGGCAC TATGTGCCAC ACAGGCCTGC CCAGAAGGCT GGTCGCCCTG GTCTGAGTGG
    AGTAAGTGCA CTGACGACGG AGCCCAGAGC CGAAGCCGGC ACTGTGAGGA GCTCCTCCCA
    GGGTCCAGCG CCTGTGCTGG AAACAGCAGC CAGAGCCGCC CCTGCCCCTA CAGCGAGATT
    CCCGTCATCC TGCCAGCCTC CAGCATGGAG GAGGCCACCG ACTGTGCAGG GTTCAATCTC
    ATCCACTTGG TGGCCACGGG CATCTCCTGC TTCTTGGGCT CTGGGCTCCT GACCCTAGCA
    GTGTACCTGT CTTGCCAGCA CTGCCAGCGT CAGTCCCAGG AGTCCACACT GGTCCATCCT
    GCCACCCCCA ACCATTTGCA CTACAAGGGC GGAGGCACCC CGAAGAATGA AAAGTACACA
    CCCATGGAAT TCAAGACCCT GAACAAGAAT AACTTGATCC CTGATGACAG AGCCAACTTC
    TACCCATTGC AGCAGACCAA TGTGTACACG ACTACTTACT ACCCAAGCCC CCTGAACAAA
    CACAGCTTCC GGCCCGAGGC CTCACCTGGA CAACGGTGCT TCCCCAACAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243277.1
    CDS400..3573
    Translation

    Target ORF information:

    RefSeq Version NM_001256348.1
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5B (SEMA5B), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256348.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGTGCTTG CAGGCCCCCT GGCTGTCTCG CTGTTGCTGC CCAGCCTCAC ACTGCTGGTG 
    TCCCACCTCT CCAGCTCCCA GGATGTCTCC AGTGAGCCCA GCAGTGAGCA GCAGCTGTGC
    GCCCTTAGCA AGCACCCCAC CGTGGCCTTT GAAGACCTGC AGCCGTGGGT CTCTAACTTC
    ACCTACCCTG GAGCCCGGGA TTTCTCCCAG CTGGCTTTGG ACCCCTCCGG GAACCAGCTC
    ATCGTGGGAG CCAGGAACTA CCTCTTCAGA CTCAGCCTTG CCAATGTCTC TCTTCTTCAG
    GCCACAGAGT GGGCCTCCAG TGAGGACACG CGCCGCTCCT GCCAAAGCAA AGGGAAGACT
    GAGGAGGAGT GTCAGAACTA CGTGCGAGTC CTGATCGTCG CCGGCCGGAA GGTGTTCATG
    TGTGGAACCA ATGCCTTTTC CCCCATGTGC ACCAGCAGAC AGGTGGGGAA CCTCAGCCGG
    ACTATTGAGA AGATCAATGG TGTGGCCCGC TGCCCCTATG ACCCACGCCA CAACTCCACA
    GCTGTCATCT CCTCCCAGGG GGAGCTCTAT GCAGCCACGG TCATCGACTT CTCAGGTCGG
    GACCCTGCCA TCTACCGCAG CCTGGGCAGT GGGCCACCGC TTCGCACTGC CCAATATAAC
    TCCAAGTGGC TTAATGAGCC AAACTTCGTG GCAGCCTATG ATATTGGGCT GTTTGCATAC
    TTCTTCCTGC GGGAGAACGC AGTGGAGCAC GACTGTGGAC GCACCGTGTA CTCTCGCGTG
    GCCCGCGTGT GCAAGAATGA CGTGGGGGGC CGATTCCTGC TGGAGGACAC ATGGACCACA
    TTCATGAAGG CCCGGCTCAA CTGCTCCCGC CCGGGCGAGG TCCCCTTCTA CTATAACGAG
    CTGCAGAGTG CCTTCCACTT GCCGGAGCAG GACCTCATCT ATGGAGTTTT CACAACCAAC
    GTAAACAGCA TCGCGGCTTC TGCTGTCTGC GCCTTCAACC TCAGTGCTAT CTCCCAGGCT
    TTCAATGGCC CATTTCGCTA CCAGGAGAAC CCCAGGGCTG CCTGGCTCCC CATAGCCAAC
    CCCATCCCCA ATTTCCAGTG TGGCACCCTG CCTGAGACCG GTCCCAACGA GAACCTGACG
    GAGCGCAGCC TGCAGGACGC GCAGCGCCTC TTCCTGATGA GCGAGGCCGT GCAGCCGGTG
    ACACCCGAGC CCTGTGTCAC CCAGGACAGC GTGCGCTTCT CACACCTCGT GGTGGACCTG
    GTGCAGGCTA AAGACACGCT CTACCATGTA CTCTACATTG GCACCGAGTC GGGCACCATC
    CTGAAGGCGC TGTCCACGGC GAGCCGCAGC CTCCACGGCT GCTACCTGGA GGAGCTGCAC
    GTGCTGCCCC CCGGGCGCCG CGAGCCCCTG CGCAGCCTGC GCATCCTGCA CAGCGCCCGC
    GCGCTCTTCG TGGGGCTGAG AGACGGCGTC CTGCGGGTCC CACTGGAGAG GTGCGCCGCC
    TACCGCAGCC AGGGGGCATG CCTGGGGGCC CGGGACCCGT ACTGTGGCTG GGACGGGAAG
    CAGCAACGTT GCAGCACACT CGAGGACAGC TCCAACATGA GCCTCTGGAC CCAGAACATC
    ACCGCCTGTC CTGTGCGGAA TGTGACACGG GATGGGGGCT TCGGCCCATG GTCACCATGG
    CAACCATGTG AGCACTTGGA TGGGGACAAC TCAGGCTCTT GCCTGTGTCG AGCTCGATCC
    TGTGATTCCC CTCGACCCCG CTGTGGGGGC CTTGACTGCC TGGGGCCAGC CATCCACATC
    GCCAACTGCT CCAGGAATGG GGCGTGGACC CCGTGGTCAT CGTGGGCGCT GTGCAGCACG
    TCCTGTGGCA TCGGCTTCCA GGTCCGCCAG CGAAGTTGCA GCAACCCTGC TCCCCGCCAC
    GGGGGCCGCA TCTGCGTGGG CAAGAGCCGG GAGGAACGGT TCTGTAATGA GAACACGCCT
    TGCCCGGTGC CCATCTTCTG GGCTTCCTGG GGCTCCTGGA GCAAGTGCAG CAGCAACTGT
    GGAGGGGGCA TGCAGTCGCG GCGTCGGGCC TGCGAGAACG GCAACTCCTG CCTGGGCTGC
    GGCGTGGAGT TCAAGACGTG CAACCCCGAG GGCTGCCCCG AAGTGCGGCG CAACACCCCC
    TGGACGCCGT GGCTGCCCGT GAACGTGACG CAGGGCGGGG CACGGCAGGA GCAGCGGTTC
    CGCTTCACCT GCCGCGCGCC CCTTGCAGAC CCGCACGGCC TGCAGTTCGG CAGGAGAAGG
    ACCGAGACGA GGACCTGTCC CGCGGACGGC TCCGGCTCCT GCGACACCGA CGCCCTGGTG
    GAGGTCCTCC TGCGCAGCGG GAGCACCTCC CCGCACACGG TGAGCGGGGG CTGGGCCGCC
    TGGGGCCCGT GGTCGTCCTG CTCCCGGGAC TGCGAGCTGG GCTTCCGCGT CCGCAAGAGA
    ACGTGCACTA ACCCGGAGCC CCGCAACGGG GGCCTGCCCT GCGTGGGCGA TGCTGCCGAG
    TACCAGGACT GCAACCCCCA GGCTTGCCCA GGTGAGGACA TCTGTCTCGG GCTGCACACG
    GAGGAGGCAC TATGTGCCAC ACAGGCCTGC CCAGAAGGCT GGTCGCCCTG GTCTGAGTGG
    AGTAAGTGCA CTGACGACGG AGCCCAGAGC CGAAGCCGGC ACTGTGAGGA GCTCCTCCCA
    GGGTCCAGCG CCTGTGCTGG AAACAGCAGC CAGAGCCGCC CCTGCCCCTA CAGCGAGATT
    CCCGTCATCC TGCCAGCCTC CAGCATGGAG GAGGCCACCG ACTGTGCAGG GTTCAATCTC
    ATCCACTTGG TGGCCACGGG CATCTCCTGC TTCTTGGGCT CTGGGCTCCT GACCCTAGCA
    GTGTACCTGT CTTGCCAGCA CTGCCAGCGT CAGTCCCAGG AGTCCACACT GGTCCATCCT
    GCCACCCCCA ACCATTTGCA CTACAAGGGC GGAGGCACCC CGAAGAATGA AAAGTACACA
    CCCATGGAAT TCAAGACCCT GAACAAGAAT AACTTGATCC CTGATGACAG AGCCAACTTC
    TACCCATTGC AGCAGACCAA TGTGTACACG ACTACTTACT ACCCAAGCCC CCTGAACAAA
    CACAGCTTCC GGCCCGAGGC CTCACCTGGA CAACGGTGCT TCCCCAACAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01368
    Clone ID Related Accession (Same CDS sequence) NM_001031702.4 , NM_001031702.3 , NM_001256346.1
    Accession Version NM_001031702.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-24
    Organism Homo sapiens(human)
    Product semaphorin-5B isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA115188.1, DA797213.1, AB040878.1, BC077726.1, AK091481.1, BC002776.2 and AC078794.13. On May 17, 2019 this sequence version replaced NM_001031702.3. Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 2 encode the same protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AB040878.1, BC077726.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000357599.8/ ENSP00000350215.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCCTGTG GCTTCAGTCC GTCTCCTGTT GCCCACCACC TCGTCCCTGG GCCGCCTGAT 
    ACCCCAGCCC AACAGCTAAG GTGTGGATGG ACAGTAGGGG GCTGGCTTCT CTCACTGGTC
    AGGGGTCTTC TCCCCTGTCT GCCTCCCGGA GCTAGGACTG CAGAGGGGCC TATCATGGTG
    CTTGCAGGCC CCCTGGCTGT CTCGCTGTTG CTGCCCAGCC TCACACTGCT GGTGTCCCAC
    CTCTCCAGCT CCCAGGATGT CTCCAGTGAG CCCAGCAGTG AGCAGCAGCT GTGCGCCCTT
    AGCAAGCACC CCACCGTGGC CTTTGAAGAC CTGCAGCCGT GGGTCTCTAA CTTCACCTAC
    CCTGGAGCCC GGGATTTCTC CCAGCTGGCT TTGGACCCCT CCGGGAACCA GCTCATCGTG
    GGAGCCAGGA ACTACCTCTT CAGACTCAGC CTTGCCAATG TCTCTCTTCT TCAGGCCACA
    GAGTGGGCCT CCAGTGAGGA CACGCGCCGC TCCTGCCAAA GCAAAGGGAA GACTGAGGAG
    GAGTGTCAGA ACTACGTGCG AGTCCTGATC GTCGCCGGCC GGAAGGTGTT CATGTGTGGA
    ACCAATGCCT TTTCCCCCAT GTGCACCAGC AGACAGGTGG GGAACCTCAG CCGGACTATT
    GAGAAGATCA ATGGTGTGGC CCGCTGCCCC TATGACCCAC GCCACAACTC CACAGCTGTC
    ATCTCCTCCC AGGGGGAGCT CTATGCAGCC ACGGTCATCG ACTTCTCAGG TCGGGACCCT
    GCCATCTACC GCAGCCTGGG CAGTGGGCCA CCGCTTCGCA CTGCCCAATA TAACTCCAAG
    TGGCTTAATG AGCCAAACTT CGTGGCAGCC TATGATATTG GGCTGTTTGC ATACTTCTTC
    CTGCGGGAGA ACGCAGTGGA GCACGACTGT GGACGCACCG TGTACTCTCG CGTGGCCCGC
    GTGTGCAAGA ATGACGTGGG GGGCCGATTC CTGCTGGAGG ACACATGGAC CACATTCATG
    AAGGCCCGGC TCAACTGCTC CCGCCCGGGC GAGGTCCCCT TCTACTATAA CGAGCTGCAG
    AGTGCCTTCC ACTTGCCGGA GCAGGACCTC ATCTATGGAG TTTTCACAAC CAACGTAAAC
    AGCATCGCGG CTTCTGCTGT CTGCGCCTTC AACCTCAGTG CTATCTCCCA GGCTTTCAAT
    GGCCCATTTC GCTACCAGGA GAACCCCAGG GCTGCCTGGC TCCCCATAGC CAACCCCATC
    CCCAATTTCC AGTGTGGCAC CCTGCCTGAG ACCGGTCCCA ACGAGAACCT GACGGAGCGC
    AGCCTGCAGG ACGCGCAGCG CCTCTTCCTG ATGAGCGAGG CCGTGCAGCC GGTGACACCC
    GAGCCCTGTG TCACCCAGGA CAGCGTGCGC TTCTCACACC TCGTGGTGGA CCTGGTGCAG
    GCTAAAGACA CGCTCTACCA TGTACTCTAC ATTGGCACCG AGTCGGGCAC CATCCTGAAG
    GCGCTGTCCA CGGCGAGCCG CAGCCTCCAC GGCTGCTACC TGGAGGAGCT GCACGTGCTG
    CCCCCCGGGC GCCGCGAGCC CCTGCGCAGC CTGCGCATCC TGCACAGCGC CCGCGCGCTC
    TTCGTGGGGC TGAGAGACGG CGTCCTGCGG GTCCCACTGG AGAGGTGCGC CGCCTACCGC
    AGCCAGGGGG CATGCCTGGG GGCCCGGGAC CCGTACTGTG GCTGGGACGG GAAGCAGCAA
    CGTTGCAGCA CACTCGAGGA CAGCTCCAAC ATGAGCCTCT GGACCCAGAA CATCACCGCC
    TGTCCTGTGC GGAATGTGAC ACGGGATGGG GGCTTCGGCC CATGGTCACC ATGGCAACCA
    TGTGAGCACT TGGATGGGGA CAACTCAGGC TCTTGCCTGT GTCGAGCTCG ATCCTGTGAT
    TCCCCTCGAC CCCGCTGTGG GGGCCTTGAC TGCCTGGGGC CAGCCATCCA CATCGCCAAC
    TGCTCCAGGA ATGGGGCGTG GACCCCGTGG TCATCGTGGG CGCTGTGCAG CACGTCCTGT
    GGCATCGGCT TCCAGGTCCG CCAGCGAAGT TGCAGCAACC CTGCTCCCCG CCACGGGGGC
    CGCATCTGCG TGGGCAAGAG CCGGGAGGAA CGGTTCTGTA ATGAGAACAC GCCTTGCCCG
    GTGCCCATCT TCTGGGCTTC CTGGGGCTCC TGGAGCAAGT GCAGCAGCAA CTGTGGAGGG
    GGCATGCAGT CGCGGCGTCG GGCCTGCGAG AACGGCAACT CCTGCCTGGG CTGCGGCGTG
    GAGTTCAAGA CGTGCAACCC CGAGGGCTGC CCCGAAGTGC GGCGCAACAC CCCCTGGACG
    CCGTGGCTGC CCGTGAACGT GACGCAGGGC GGGGCACGGC AGGAGCAGCG GTTCCGCTTC
    ACCTGCCGCG CGCCCCTTGC AGACCCGCAC GGCCTGCAGT TCGGCAGGAG AAGGACCGAG
    ACGAGGACCT GTCCCGCGGA CGGCTCCGGC TCCTGCGACA CCGACGCCCT GGTGGAGGTC
    CTCCTGCGCA GCGGGAGCAC CTCCCCGCAC ACGGTGAGCG GGGGCTGGGC CGCCTGGGGC
    CCGTGGTCGT CCTGCTCCCG GGACTGCGAG CTGGGCTTCC GCGTCCGCAA GAGAACGTGC
    ACTAACCCGG AGCCCCGCAA CGGGGGCCTG CCCTGCGTGG GCGATGCTGC CGAGTACCAG
    GACTGCAACC CCCAGGCTTG CCCAGTTCGG GGTGCTTGGT CCTGCTGGAC CTCATGGTCT
    CCATGCTCAG CTTCCTGTGG TGGGGGTCAC TATCAACGCA CCCGTTCCTG CACCAGCCCC
    GCACCCTCCC CAGGTGAGGA CATCTGTCTC GGGCTGCACA CGGAGGAGGC ACTATGTGCC
    ACACAGGCCT GCCCAGAAGG CTGGTCGCCC TGGTCTGAGT GGAGTAAGTG CACTGACGAC
    GGAGCCCAGA GCCGAAGCCG GCACTGTGAG GAGCTCCTCC CAGGGTCCAG CGCCTGTGCT
    GGAAACAGCA GCCAGAGCCG CCCCTGCCCC TACAGCGAGA TTCCCGTCAT CCTGCCAGCC
    TCCAGCATGG AGGAGGCCAC CGACTGTGCA GGGTTCAATC TCATCCACTT GGTGGCCACG
    GGCATCTCCT GCTTCTTGGG CTCTGGGCTC CTGACCCTAG CAGTGTACCT GTCTTGCCAG
    CACTGCCAGC GTCAGTCCCA GGAGTCCACA CTGGTCCATC CTGCCACCCC CAACCATTTG
    CACTACAAGG GCGGAGGCAC CCCGAAGAAT GAAAAGTACA CACCCATGGA ATTCAAGACC
    CTGAACAAGA ATAACTTGAT CCCTGATGAC AGAGCCAACT TCTACCCATT GCAGCAGACC
    AATGTGTACA CGACTACTTA CTACCCAAGC CCCCTGAACA AACACAGCTT CCGGCCCGAG
    GCCTCACCTG GACAACGGTG CTTCCCCAAC AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001026872.2
    CDS339..3794
    Translation

    Target ORF information:

    RefSeq Version NM_001031702.4
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5B (SEMA5B), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001031702.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCCTGTG GCTTCAGTCC GTCTCCTGTT GCCCACCACC TCGTCCCTGG GCCGCCTGAT 
    ACCCCAGCCC AACAGCTAAG GTGTGGATGG ACAGTAGGGG GCTGGCTTCT CTCACTGGTC
    AGGGGTCTTC TCCCCTGTCT GCCTCCCGGA GCTAGGACTG CAGAGGGGCC TATCATGGTG
    CTTGCAGGCC CCCTGGCTGT CTCGCTGTTG CTGCCCAGCC TCACACTGCT GGTGTCCCAC
    CTCTCCAGCT CCCAGGATGT CTCCAGTGAG CCCAGCAGTG AGCAGCAGCT GTGCGCCCTT
    AGCAAGCACC CCACCGTGGC CTTTGAAGAC CTGCAGCCGT GGGTCTCTAA CTTCACCTAC
    CCTGGAGCCC GGGATTTCTC CCAGCTGGCT TTGGACCCCT CCGGGAACCA GCTCATCGTG
    GGAGCCAGGA ACTACCTCTT CAGACTCAGC CTTGCCAATG TCTCTCTTCT TCAGGCCACA
    GAGTGGGCCT CCAGTGAGGA CACGCGCCGC TCCTGCCAAA GCAAAGGGAA GACTGAGGAG
    GAGTGTCAGA ACTACGTGCG AGTCCTGATC GTCGCCGGCC GGAAGGTGTT CATGTGTGGA
    ACCAATGCCT TTTCCCCCAT GTGCACCAGC AGACAGGTGG GGAACCTCAG CCGGACTATT
    GAGAAGATCA ATGGTGTGGC CCGCTGCCCC TATGACCCAC GCCACAACTC CACAGCTGTC
    ATCTCCTCCC AGGGGGAGCT CTATGCAGCC ACGGTCATCG ACTTCTCAGG TCGGGACCCT
    GCCATCTACC GCAGCCTGGG CAGTGGGCCA CCGCTTCGCA CTGCCCAATA TAACTCCAAG
    TGGCTTAATG AGCCAAACTT CGTGGCAGCC TATGATATTG GGCTGTTTGC ATACTTCTTC
    CTGCGGGAGA ACGCAGTGGA GCACGACTGT GGACGCACCG TGTACTCTCG CGTGGCCCGC
    GTGTGCAAGA ATGACGTGGG GGGCCGATTC CTGCTGGAGG ACACATGGAC CACATTCATG
    AAGGCCCGGC TCAACTGCTC CCGCCCGGGC GAGGTCCCCT TCTACTATAA CGAGCTGCAG
    AGTGCCTTCC ACTTGCCGGA GCAGGACCTC ATCTATGGAG TTTTCACAAC CAACGTAAAC
    AGCATCGCGG CTTCTGCTGT CTGCGCCTTC AACCTCAGTG CTATCTCCCA GGCTTTCAAT
    GGCCCATTTC GCTACCAGGA GAACCCCAGG GCTGCCTGGC TCCCCATAGC CAACCCCATC
    CCCAATTTCC AGTGTGGCAC CCTGCCTGAG ACCGGTCCCA ACGAGAACCT GACGGAGCGC
    AGCCTGCAGG ACGCGCAGCG CCTCTTCCTG ATGAGCGAGG CCGTGCAGCC GGTGACACCC
    GAGCCCTGTG TCACCCAGGA CAGCGTGCGC TTCTCACACC TCGTGGTGGA CCTGGTGCAG
    GCTAAAGACA CGCTCTACCA TGTACTCTAC ATTGGCACCG AGTCGGGCAC CATCCTGAAG
    GCGCTGTCCA CGGCGAGCCG CAGCCTCCAC GGCTGCTACC TGGAGGAGCT GCACGTGCTG
    CCCCCCGGGC GCCGCGAGCC CCTGCGCAGC CTGCGCATCC TGCACAGCGC CCGCGCGCTC
    TTCGTGGGGC TGAGAGACGG CGTCCTGCGG GTCCCACTGG AGAGGTGCGC CGCCTACCGC
    AGCCAGGGGG CATGCCTGGG GGCCCGGGAC CCGTACTGTG GCTGGGACGG GAAGCAGCAA
    CGTTGCAGCA CACTCGAGGA CAGCTCCAAC ATGAGCCTCT GGACCCAGAA CATCACCGCC
    TGTCCTGTGC GGAATGTGAC ACGGGATGGG GGCTTCGGCC CATGGTCACC ATGGCAACCA
    TGTGAGCACT TGGATGGGGA CAACTCAGGC TCTTGCCTGT GTCGAGCTCG ATCCTGTGAT
    TCCCCTCGAC CCCGCTGTGG GGGCCTTGAC TGCCTGGGGC CAGCCATCCA CATCGCCAAC
    TGCTCCAGGA ATGGGGCGTG GACCCCGTGG TCATCGTGGG CGCTGTGCAG CACGTCCTGT
    GGCATCGGCT TCCAGGTCCG CCAGCGAAGT TGCAGCAACC CTGCTCCCCG CCACGGGGGC
    CGCATCTGCG TGGGCAAGAG CCGGGAGGAA CGGTTCTGTA ATGAGAACAC GCCTTGCCCG
    GTGCCCATCT TCTGGGCTTC CTGGGGCTCC TGGAGCAAGT GCAGCAGCAA CTGTGGAGGG
    GGCATGCAGT CGCGGCGTCG GGCCTGCGAG AACGGCAACT CCTGCCTGGG CTGCGGCGTG
    GAGTTCAAGA CGTGCAACCC CGAGGGCTGC CCCGAAGTGC GGCGCAACAC CCCCTGGACG
    CCGTGGCTGC CCGTGAACGT GACGCAGGGC GGGGCACGGC AGGAGCAGCG GTTCCGCTTC
    ACCTGCCGCG CGCCCCTTGC AGACCCGCAC GGCCTGCAGT TCGGCAGGAG AAGGACCGAG
    ACGAGGACCT GTCCCGCGGA CGGCTCCGGC TCCTGCGACA CCGACGCCCT GGTGGAGGTC
    CTCCTGCGCA GCGGGAGCAC CTCCCCGCAC ACGGTGAGCG GGGGCTGGGC CGCCTGGGGC
    CCGTGGTCGT CCTGCTCCCG GGACTGCGAG CTGGGCTTCC GCGTCCGCAA GAGAACGTGC
    ACTAACCCGG AGCCCCGCAA CGGGGGCCTG CCCTGCGTGG GCGATGCTGC CGAGTACCAG
    GACTGCAACC CCCAGGCTTG CCCAGTTCGG GGTGCTTGGT CCTGCTGGAC CTCATGGTCT
    CCATGCTCAG CTTCCTGTGG TGGGGGTCAC TATCAACGCA CCCGTTCCTG CACCAGCCCC
    GCACCCTCCC CAGGTGAGGA CATCTGTCTC GGGCTGCACA CGGAGGAGGC ACTATGTGCC
    ACACAGGCCT GCCCAGAAGG CTGGTCGCCC TGGTCTGAGT GGAGTAAGTG CACTGACGAC
    GGAGCCCAGA GCCGAAGCCG GCACTGTGAG GAGCTCCTCC CAGGGTCCAG CGCCTGTGCT
    GGAAACAGCA GCCAGAGCCG CCCCTGCCCC TACAGCGAGA TTCCCGTCAT CCTGCCAGCC
    TCCAGCATGG AGGAGGCCAC CGACTGTGCA GGGTTCAATC TCATCCACTT GGTGGCCACG
    GGCATCTCCT GCTTCTTGGG CTCTGGGCTC CTGACCCTAG CAGTGTACCT GTCTTGCCAG
    CACTGCCAGC GTCAGTCCCA GGAGTCCACA CTGGTCCATC CTGCCACCCC CAACCATTTG
    CACTACAAGG GCGGAGGCAC CCCGAAGAAT GAAAAGTACA CACCCATGGA ATTCAAGACC
    CTGAACAAGA ATAACTTGAT CCCTGATGAC AGAGCCAACT TCTACCCATT GCAGCAGACC
    AATGTGTACA CGACTACTTA CTACCCAAGC CCCCTGAACA AACACAGCTT CCGGCCCGAG
    GCCTCACCTG GACAACGGTG CTTCCCCAAC AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01368
    Clone ID Related Accession (Same CDS sequence) NM_001031702.4 , NM_001031702.3 , NM_001256346.1
    Accession Version NM_001031702.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product semaphorin-5B isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA115188.1, DA797213.1, AB040878.1, BC077726.1, AK091481.1, BC002776.2 and AC078794.13. On Jan 21, 2012 this sequence version replaced NM_001031702.2. Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 2 encode the same protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AB040878.1, BC077726.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCCTGTG GCTTCAGTCC GTCTCCTGTT GCCCACCACC TCGTCCCTGG GCCGCCTGAT 
    ACCCCAGCCC AACAGCTAAG GTGTGGATGG ACAGTAGGGG GCTGGCTTCT CTCACTGGTC
    AGGGGTCTTC TCCCCTGTCT GCCTCCCGGA GCTAGGACTG CAGAGGGGCC TATCATGGTG
    CTTGCAGGCC CCCTGGCTGT CTCGCTGTTG CTGCCCAGCC TCACACTGCT GGTGTCCCAC
    CTCTCCAGCT CCCAGGATGT CTCCAGTGAG CCCAGCAGTG AGCAGCAGCT GTGCGCCCTT
    AGCAAGCACC CCACCGTGGC CTTTGAAGAC CTGCAGCCGT GGGTCTCTAA CTTCACCTAC
    CCTGGAGCCC GGGATTTCTC CCAGCTGGCT TTGGACCCCT CCGGGAACCA GCTCATCGTG
    GGAGCCAGGA ACTACCTCTT CAGACTCAGC CTTGCCAATG TCTCTCTTCT TCAGGCCACA
    GAGTGGGCCT CCAGTGAGGA CACGCGCCGC TCCTGCCAAA GCAAAGGGAA GACTGAGGAG
    GAGTGTCAGA ACTACGTGCG AGTCCTGATC GTCGCCGGCC GGAAGGTGTT CATGTGTGGA
    ACCAATGCCT TTTCCCCCAT GTGCACCAGC AGACAGGTGG GGAACCTCAG CCGGACTATT
    GAGAAGATCA ATGGTGTGGC CCGCTGCCCC TATGACCCAC GCCACAACTC CACAGCTGTC
    ATCTCCTCCC AGGGGGAGCT CTATGCAGCC ACGGTCATCG ACTTCTCAGG TCGGGACCCT
    GCCATCTACC GCAGCCTGGG CAGTGGGCCA CCGCTTCGCA CTGCCCAATA TAACTCCAAG
    TGGCTTAATG AGCCAAACTT CGTGGCAGCC TATGATATTG GGCTGTTTGC ATACTTCTTC
    CTGCGGGAGA ACGCAGTGGA GCACGACTGT GGACGCACCG TGTACTCTCG CGTGGCCCGC
    GTGTGCAAGA ATGACGTGGG GGGCCGATTC CTGCTGGAGG ACACATGGAC CACATTCATG
    AAGGCCCGGC TCAACTGCTC CCGCCCGGGC GAGGTCCCCT TCTACTATAA CGAGCTGCAG
    AGTGCCTTCC ACTTGCCGGA GCAGGACCTC ATCTATGGAG TTTTCACAAC CAACGTAAAC
    AGCATCGCGG CTTCTGCTGT CTGCGCCTTC AACCTCAGTG CTATCTCCCA GGCTTTCAAT
    GGCCCATTTC GCTACCAGGA GAACCCCAGG GCTGCCTGGC TCCCCATAGC CAACCCCATC
    CCCAATTTCC AGTGTGGCAC CCTGCCTGAG ACCGGTCCCA ACGAGAACCT GACGGAGCGC
    AGCCTGCAGG ACGCGCAGCG CCTCTTCCTG ATGAGCGAGG CCGTGCAGCC GGTGACACCC
    GAGCCCTGTG TCACCCAGGA CAGCGTGCGC TTCTCACACC TCGTGGTGGA CCTGGTGCAG
    GCTAAAGACA CGCTCTACCA TGTACTCTAC ATTGGCACCG AGTCGGGCAC CATCCTGAAG
    GCGCTGTCCA CGGCGAGCCG CAGCCTCCAC GGCTGCTACC TGGAGGAGCT GCACGTGCTG
    CCCCCCGGGC GCCGCGAGCC CCTGCGCAGC CTGCGCATCC TGCACAGCGC CCGCGCGCTC
    TTCGTGGGGC TGAGAGACGG CGTCCTGCGG GTCCCACTGG AGAGGTGCGC CGCCTACCGC
    AGCCAGGGGG CATGCCTGGG GGCCCGGGAC CCGTACTGTG GCTGGGACGG GAAGCAGCAA
    CGTTGCAGCA CACTCGAGGA CAGCTCCAAC ATGAGCCTCT GGACCCAGAA CATCACCGCC
    TGTCCTGTGC GGAATGTGAC ACGGGATGGG GGCTTCGGCC CATGGTCACC ATGGCAACCA
    TGTGAGCACT TGGATGGGGA CAACTCAGGC TCTTGCCTGT GTCGAGCTCG ATCCTGTGAT
    TCCCCTCGAC CCCGCTGTGG GGGCCTTGAC TGCCTGGGGC CAGCCATCCA CATCGCCAAC
    TGCTCCAGGA ATGGGGCGTG GACCCCGTGG TCATCGTGGG CGCTGTGCAG CACGTCCTGT
    GGCATCGGCT TCCAGGTCCG CCAGCGAAGT TGCAGCAACC CTGCTCCCCG CCACGGGGGC
    CGCATCTGCG TGGGCAAGAG CCGGGAGGAA CGGTTCTGTA ATGAGAACAC GCCTTGCCCG
    GTGCCCATCT TCTGGGCTTC CTGGGGCTCC TGGAGCAAGT GCAGCAGCAA CTGTGGAGGG
    GGCATGCAGT CGCGGCGTCG GGCCTGCGAG AACGGCAACT CCTGCCTGGG CTGCGGCGTG
    GAGTTCAAGA CGTGCAACCC CGAGGGCTGC CCCGAAGTGC GGCGCAACAC CCCCTGGACG
    CCGTGGCTGC CCGTGAACGT GACGCAGGGC GGGGCACGGC AGGAGCAGCG GTTCCGCTTC
    ACCTGCCGCG CGCCCCTTGC AGACCCGCAC GGCCTGCAGT TCGGCAGGAG AAGGACCGAG
    ACGAGGACCT GTCCCGCGGA CGGCTCCGGC TCCTGCGACA CCGACGCCCT GGTGGAGGTC
    CTCCTGCGCA GCGGGAGCAC CTCCCCGCAC ACGGTGAGCG GGGGCTGGGC CGCCTGGGGC
    CCGTGGTCGT CCTGCTCCCG GGACTGCGAG CTGGGCTTCC GCGTCCGCAA GAGAACGTGC
    ACTAACCCGG AGCCCCGCAA CGGGGGCCTG CCCTGCGTGG GCGATGCTGC CGAGTACCAG
    GACTGCAACC CCCAGGCTTG CCCAGTTCGG GGTGCTTGGT CCTGCTGGAC CTCATGGTCT
    CCATGCTCAG CTTCCTGTGG TGGGGGTCAC TATCAACGCA CCCGTTCCTG CACCAGCCCC
    GCACCCTCCC CAGGTGAGGA CATCTGTCTC GGGCTGCACA CGGAGGAGGC ACTATGTGCC
    ACACAGGCCT GCCCAGAAGG CTGGTCGCCC TGGTCTGAGT GGAGTAAGTG CACTGACGAC
    GGAGCCCAGA GCCGAAGCCG GCACTGTGAG GAGCTCCTCC CAGGGTCCAG CGCCTGTGCT
    GGAAACAGCA GCCAGAGCCG CCCCTGCCCC TACAGCGAGA TTCCCGTCAT CCTGCCAGCC
    TCCAGCATGG AGGAGGCCAC CGACTGTGCA GGGTTCAATC TCATCCACTT GGTGGCCACG
    GGCATCTCCT GCTTCTTGGG CTCTGGGCTC CTGACCCTAG CAGTGTACCT GTCTTGCCAG
    CACTGCCAGC GTCAGTCCCA GGAGTCCACA CTGGTCCATC CTGCCACCCC CAACCATTTG
    CACTACAAGG GCGGAGGCAC CCCGAAGAAT GAAAAGTACA CACCCATGGA ATTCAAGACC
    CTGAACAAGA ATAACTTGAT CCCTGATGAC AGAGCCAACT TCTACCCATT GCAGCAGACC
    AATGTGTACA CGACTACTTA CTACCCAAGC CCCCTGAACA AACACAGCTT CCGGCCCGAG
    GCCTCACCTG GACAACGGTG CTTCCCCAAC AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001026872.2
    CDS388..3843
    Translation

    Target ORF information:

    RefSeq Version NM_001031702.3
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5B (SEMA5B), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001031702.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCCTGTG GCTTCAGTCC GTCTCCTGTT GCCCACCACC TCGTCCCTGG GCCGCCTGAT 
    ACCCCAGCCC AACAGCTAAG GTGTGGATGG ACAGTAGGGG GCTGGCTTCT CTCACTGGTC
    AGGGGTCTTC TCCCCTGTCT GCCTCCCGGA GCTAGGACTG CAGAGGGGCC TATCATGGTG
    CTTGCAGGCC CCCTGGCTGT CTCGCTGTTG CTGCCCAGCC TCACACTGCT GGTGTCCCAC
    CTCTCCAGCT CCCAGGATGT CTCCAGTGAG CCCAGCAGTG AGCAGCAGCT GTGCGCCCTT
    AGCAAGCACC CCACCGTGGC CTTTGAAGAC CTGCAGCCGT GGGTCTCTAA CTTCACCTAC
    CCTGGAGCCC GGGATTTCTC CCAGCTGGCT TTGGACCCCT CCGGGAACCA GCTCATCGTG
    GGAGCCAGGA ACTACCTCTT CAGACTCAGC CTTGCCAATG TCTCTCTTCT TCAGGCCACA
    GAGTGGGCCT CCAGTGAGGA CACGCGCCGC TCCTGCCAAA GCAAAGGGAA GACTGAGGAG
    GAGTGTCAGA ACTACGTGCG AGTCCTGATC GTCGCCGGCC GGAAGGTGTT CATGTGTGGA
    ACCAATGCCT TTTCCCCCAT GTGCACCAGC AGACAGGTGG GGAACCTCAG CCGGACTATT
    GAGAAGATCA ATGGTGTGGC CCGCTGCCCC TATGACCCAC GCCACAACTC CACAGCTGTC
    ATCTCCTCCC AGGGGGAGCT CTATGCAGCC ACGGTCATCG ACTTCTCAGG TCGGGACCCT
    GCCATCTACC GCAGCCTGGG CAGTGGGCCA CCGCTTCGCA CTGCCCAATA TAACTCCAAG
    TGGCTTAATG AGCCAAACTT CGTGGCAGCC TATGATATTG GGCTGTTTGC ATACTTCTTC
    CTGCGGGAGA ACGCAGTGGA GCACGACTGT GGACGCACCG TGTACTCTCG CGTGGCCCGC
    GTGTGCAAGA ATGACGTGGG GGGCCGATTC CTGCTGGAGG ACACATGGAC CACATTCATG
    AAGGCCCGGC TCAACTGCTC CCGCCCGGGC GAGGTCCCCT TCTACTATAA CGAGCTGCAG
    AGTGCCTTCC ACTTGCCGGA GCAGGACCTC ATCTATGGAG TTTTCACAAC CAACGTAAAC
    AGCATCGCGG CTTCTGCTGT CTGCGCCTTC AACCTCAGTG CTATCTCCCA GGCTTTCAAT
    GGCCCATTTC GCTACCAGGA GAACCCCAGG GCTGCCTGGC TCCCCATAGC CAACCCCATC
    CCCAATTTCC AGTGTGGCAC CCTGCCTGAG ACCGGTCCCA ACGAGAACCT GACGGAGCGC
    AGCCTGCAGG ACGCGCAGCG CCTCTTCCTG ATGAGCGAGG CCGTGCAGCC GGTGACACCC
    GAGCCCTGTG TCACCCAGGA CAGCGTGCGC TTCTCACACC TCGTGGTGGA CCTGGTGCAG
    GCTAAAGACA CGCTCTACCA TGTACTCTAC ATTGGCACCG AGTCGGGCAC CATCCTGAAG
    GCGCTGTCCA CGGCGAGCCG CAGCCTCCAC GGCTGCTACC TGGAGGAGCT GCACGTGCTG
    CCCCCCGGGC GCCGCGAGCC CCTGCGCAGC CTGCGCATCC TGCACAGCGC CCGCGCGCTC
    TTCGTGGGGC TGAGAGACGG CGTCCTGCGG GTCCCACTGG AGAGGTGCGC CGCCTACCGC
    AGCCAGGGGG CATGCCTGGG GGCCCGGGAC CCGTACTGTG GCTGGGACGG GAAGCAGCAA
    CGTTGCAGCA CACTCGAGGA CAGCTCCAAC ATGAGCCTCT GGACCCAGAA CATCACCGCC
    TGTCCTGTGC GGAATGTGAC ACGGGATGGG GGCTTCGGCC CATGGTCACC ATGGCAACCA
    TGTGAGCACT TGGATGGGGA CAACTCAGGC TCTTGCCTGT GTCGAGCTCG ATCCTGTGAT
    TCCCCTCGAC CCCGCTGTGG GGGCCTTGAC TGCCTGGGGC CAGCCATCCA CATCGCCAAC
    TGCTCCAGGA ATGGGGCGTG GACCCCGTGG TCATCGTGGG CGCTGTGCAG CACGTCCTGT
    GGCATCGGCT TCCAGGTCCG CCAGCGAAGT TGCAGCAACC CTGCTCCCCG CCACGGGGGC
    CGCATCTGCG TGGGCAAGAG CCGGGAGGAA CGGTTCTGTA ATGAGAACAC GCCTTGCCCG
    GTGCCCATCT TCTGGGCTTC CTGGGGCTCC TGGAGCAAGT GCAGCAGCAA CTGTGGAGGG
    GGCATGCAGT CGCGGCGTCG GGCCTGCGAG AACGGCAACT CCTGCCTGGG CTGCGGCGTG
    GAGTTCAAGA CGTGCAACCC CGAGGGCTGC CCCGAAGTGC GGCGCAACAC CCCCTGGACG
    CCGTGGCTGC CCGTGAACGT GACGCAGGGC GGGGCACGGC AGGAGCAGCG GTTCCGCTTC
    ACCTGCCGCG CGCCCCTTGC AGACCCGCAC GGCCTGCAGT TCGGCAGGAG AAGGACCGAG
    ACGAGGACCT GTCCCGCGGA CGGCTCCGGC TCCTGCGACA CCGACGCCCT GGTGGAGGTC
    CTCCTGCGCA GCGGGAGCAC CTCCCCGCAC ACGGTGAGCG GGGGCTGGGC CGCCTGGGGC
    CCGTGGTCGT CCTGCTCCCG GGACTGCGAG CTGGGCTTCC GCGTCCGCAA GAGAACGTGC
    ACTAACCCGG AGCCCCGCAA CGGGGGCCTG CCCTGCGTGG GCGATGCTGC CGAGTACCAG
    GACTGCAACC CCCAGGCTTG CCCAGTTCGG GGTGCTTGGT CCTGCTGGAC CTCATGGTCT
    CCATGCTCAG CTTCCTGTGG TGGGGGTCAC TATCAACGCA CCCGTTCCTG CACCAGCCCC
    GCACCCTCCC CAGGTGAGGA CATCTGTCTC GGGCTGCACA CGGAGGAGGC ACTATGTGCC
    ACACAGGCCT GCCCAGAAGG CTGGTCGCCC TGGTCTGAGT GGAGTAAGTG CACTGACGAC
    GGAGCCCAGA GCCGAAGCCG GCACTGTGAG GAGCTCCTCC CAGGGTCCAG CGCCTGTGCT
    GGAAACAGCA GCCAGAGCCG CCCCTGCCCC TACAGCGAGA TTCCCGTCAT CCTGCCAGCC
    TCCAGCATGG AGGAGGCCAC CGACTGTGCA GGGTTCAATC TCATCCACTT GGTGGCCACG
    GGCATCTCCT GCTTCTTGGG CTCTGGGCTC CTGACCCTAG CAGTGTACCT GTCTTGCCAG
    CACTGCCAGC GTCAGTCCCA GGAGTCCACA CTGGTCCATC CTGCCACCCC CAACCATTTG
    CACTACAAGG GCGGAGGCAC CCCGAAGAAT GAAAAGTACA CACCCATGGA ATTCAAGACC
    CTGAACAAGA ATAACTTGAT CCCTGATGAC AGAGCCAACT TCTACCCATT GCAGCAGACC
    AATGTGTACA CGACTACTTA CTACCCAAGC CCCCTGAACA AACACAGCTT CCGGCCCGAG
    GCCTCACCTG GACAACGGTG CTTCCCCAAC AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu01368
    Clone ID Related Accession (Same CDS sequence) NM_001031702.4 , NM_001031702.3 , NM_001256346.1
    Accession Version NM_001256346.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-22
    Organism Homo sapiens(human)
    Product semaphorin-5B isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK091481.1, AC109130.8 and AC078794.13. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## CDS exon combination :: AB040878.1, BC077726.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCCTGTG GCTTCAGTCC GTCTCCTGTT GCCCACCACC TCGTCCCTGG GCCGCCTGAT 
    ACCCCAGCCC AACAGCTAAG GTGTGGATGG ACAGTAGGGG GCTGGCTTCT CTCACTGGTC
    AGGGGTCTTC TCCCCTGTCT GCCTCCCGGA GCTAGGACTG CAGAGGGGCC TATCATGGTG
    CTTGCAGGCC CCCTGGCTGT CTCGCTGTTG CTGCCCAGCC TCACACTGCT GGTGTCCCAC
    CTCTCCAGCT CCCAGGATGT CTCCAGTGAG CCCAGCAGTG AGCAGCAGCT GTGCGCCCTT
    AGCAAGCACC CCACCGTGGC CTTTGAAGAC CTGCAGCCGT GGGTCTCTAA CTTCACCTAC
    CCTGGAGCCC GGGATTTCTC CCAGCTGGCT TTGGACCCCT CCGGGAACCA GCTCATCGTG
    GGAGCCAGGA ACTACCTCTT CAGACTCAGC CTTGCCAATG TCTCTCTTCT TCAGGCCACA
    GAGTGGGCCT CCAGTGAGGA CACGCGCCGC TCCTGCCAAA GCAAAGGGAA GACTGAGGAG
    GAGTGTCAGA ACTACGTGCG AGTCCTGATC GTCGCCGGCC GGAAGGTGTT CATGTGTGGA
    ACCAATGCCT TTTCCCCCAT GTGCACCAGC AGACAGGTGG GGAACCTCAG CCGGACTATT
    GAGAAGATCA ATGGTGTGGC CCGCTGCCCC TATGACCCAC GCCACAACTC CACAGCTGTC
    ATCTCCTCCC AGGGGGAGCT CTATGCAGCC ACGGTCATCG ACTTCTCAGG TCGGGACCCT
    GCCATCTACC GCAGCCTGGG CAGTGGGCCA CCGCTTCGCA CTGCCCAATA TAACTCCAAG
    TGGCTTAATG AGCCAAACTT CGTGGCAGCC TATGATATTG GGCTGTTTGC ATACTTCTTC
    CTGCGGGAGA ACGCAGTGGA GCACGACTGT GGACGCACCG TGTACTCTCG CGTGGCCCGC
    GTGTGCAAGA ATGACGTGGG GGGCCGATTC CTGCTGGAGG ACACATGGAC CACATTCATG
    AAGGCCCGGC TCAACTGCTC CCGCCCGGGC GAGGTCCCCT TCTACTATAA CGAGCTGCAG
    AGTGCCTTCC ACTTGCCGGA GCAGGACCTC ATCTATGGAG TTTTCACAAC CAACGTAAAC
    AGCATCGCGG CTTCTGCTGT CTGCGCCTTC AACCTCAGTG CTATCTCCCA GGCTTTCAAT
    GGCCCATTTC GCTACCAGGA GAACCCCAGG GCTGCCTGGC TCCCCATAGC CAACCCCATC
    CCCAATTTCC AGTGTGGCAC CCTGCCTGAG ACCGGTCCCA ACGAGAACCT GACGGAGCGC
    AGCCTGCAGG ACGCGCAGCG CCTCTTCCTG ATGAGCGAGG CCGTGCAGCC GGTGACACCC
    GAGCCCTGTG TCACCCAGGA CAGCGTGCGC TTCTCACACC TCGTGGTGGA CCTGGTGCAG
    GCTAAAGACA CGCTCTACCA TGTACTCTAC ATTGGCACCG AGTCGGGCAC CATCCTGAAG
    GCGCTGTCCA CGGCGAGCCG CAGCCTCCAC GGCTGCTACC TGGAGGAGCT GCACGTGCTG
    CCCCCCGGGC GCCGCGAGCC CCTGCGCAGC CTGCGCATCC TGCACAGCGC CCGCGCGCTC
    TTCGTGGGGC TGAGAGACGG CGTCCTGCGG GTCCCACTGG AGAGGTGCGC CGCCTACCGC
    AGCCAGGGGG CATGCCTGGG GGCCCGGGAC CCGTACTGTG GCTGGGACGG GAAGCAGCAA
    CGTTGCAGCA CACTCGAGGA CAGCTCCAAC ATGAGCCTCT GGACCCAGAA CATCACCGCC
    TGTCCTGTGC GGAATGTGAC ACGGGATGGG GGCTTCGGCC CATGGTCACC ATGGCAACCA
    TGTGAGCACT TGGATGGGGA CAACTCAGGC TCTTGCCTGT GTCGAGCTCG ATCCTGTGAT
    TCCCCTCGAC CCCGCTGTGG GGGCCTTGAC TGCCTGGGGC CAGCCATCCA CATCGCCAAC
    TGCTCCAGGA ATGGGGCGTG GACCCCGTGG TCATCGTGGG CGCTGTGCAG CACGTCCTGT
    GGCATCGGCT TCCAGGTCCG CCAGCGAAGT TGCAGCAACC CTGCTCCCCG CCACGGGGGC
    CGCATCTGCG TGGGCAAGAG CCGGGAGGAA CGGTTCTGTA ATGAGAACAC GCCTTGCCCG
    GTGCCCATCT TCTGGGCTTC CTGGGGCTCC TGGAGCAAGT GCAGCAGCAA CTGTGGAGGG
    GGCATGCAGT CGCGGCGTCG GGCCTGCGAG AACGGCAACT CCTGCCTGGG CTGCGGCGTG
    GAGTTCAAGA CGTGCAACCC CGAGGGCTGC CCCGAAGTGC GGCGCAACAC CCCCTGGACG
    CCGTGGCTGC CCGTGAACGT GACGCAGGGC GGGGCACGGC AGGAGCAGCG GTTCCGCTTC
    ACCTGCCGCG CGCCCCTTGC AGACCCGCAC GGCCTGCAGT TCGGCAGGAG AAGGACCGAG
    ACGAGGACCT GTCCCGCGGA CGGCTCCGGC TCCTGCGACA CCGACGCCCT GGTGGAGGTC
    CTCCTGCGCA GCGGGAGCAC CTCCCCGCAC ACGGTGAGCG GGGGCTGGGC CGCCTGGGGC
    CCGTGGTCGT CCTGCTCCCG GGACTGCGAG CTGGGCTTCC GCGTCCGCAA GAGAACGTGC
    ACTAACCCGG AGCCCCGCAA CGGGGGCCTG CCCTGCGTGG GCGATGCTGC CGAGTACCAG
    GACTGCAACC CCCAGGCTTG CCCAGTTCGG GGTGCTTGGT CCTGCTGGAC CTCATGGTCT
    CCATGCTCAG CTTCCTGTGG TGGGGGTCAC TATCAACGCA CCCGTTCCTG CACCAGCCCC
    GCACCCTCCC CAGGTGAGGA CATCTGTCTC GGGCTGCACA CGGAGGAGGC ACTATGTGCC
    ACACAGGCCT GCCCAGAAGG CTGGTCGCCC TGGTCTGAGT GGAGTAAGTG CACTGACGAC
    GGAGCCCAGA GCCGAAGCCG GCACTGTGAG GAGCTCCTCC CAGGGTCCAG CGCCTGTGCT
    GGAAACAGCA GCCAGAGCCG CCCCTGCCCC TACAGCGAGA TTCCCGTCAT CCTGCCAGCC
    TCCAGCATGG AGGAGGCCAC CGACTGTGCA GGGTTCAATC TCATCCACTT GGTGGCCACG
    GGCATCTCCT GCTTCTTGGG CTCTGGGCTC CTGACCCTAG CAGTGTACCT GTCTTGCCAG
    CACTGCCAGC GTCAGTCCCA GGAGTCCACA CTGGTCCATC CTGCCACCCC CAACCATTTG
    CACTACAAGG GCGGAGGCAC CCCGAAGAAT GAAAAGTACA CACCCATGGA ATTCAAGACC
    CTGAACAAGA ATAACTTGAT CCCTGATGAC AGAGCCAACT TCTACCCATT GCAGCAGACC
    AATGTGTACA CGACTACTTA CTACCCAAGC CCCCTGAACA AACACAGCTT CCGGCCCGAG
    GCCTCACCTG GACAACGGTG CTTCCCCAAC AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243275.1
    CDS528..3983
    Translation

    Target ORF information:

    RefSeq Version NM_001256346.1
    Organism Homo sapiens(human)
    Definition Homo sapiens semaphorin 5B (SEMA5B), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCCTGTG GCTTCAGTCC GTCTCCTGTT GCCCACCACC TCGTCCCTGG GCCGCCTGAT 
    ACCCCAGCCC AACAGCTAAG GTGTGGATGG ACAGTAGGGG GCTGGCTTCT CTCACTGGTC
    AGGGGTCTTC TCCCCTGTCT GCCTCCCGGA GCTAGGACTG CAGAGGGGCC TATCATGGTG
    CTTGCAGGCC CCCTGGCTGT CTCGCTGTTG CTGCCCAGCC TCACACTGCT GGTGTCCCAC
    CTCTCCAGCT CCCAGGATGT CTCCAGTGAG CCCAGCAGTG AGCAGCAGCT GTGCGCCCTT
    AGCAAGCACC CCACCGTGGC CTTTGAAGAC CTGCAGCCGT GGGTCTCTAA CTTCACCTAC
    CCTGGAGCCC GGGATTTCTC CCAGCTGGCT TTGGACCCCT CCGGGAACCA GCTCATCGTG
    GGAGCCAGGA ACTACCTCTT CAGACTCAGC CTTGCCAATG TCTCTCTTCT TCAGGCCACA
    GAGTGGGCCT CCAGTGAGGA CACGCGCCGC TCCTGCCAAA GCAAAGGGAA GACTGAGGAG
    GAGTGTCAGA ACTACGTGCG AGTCCTGATC GTCGCCGGCC GGAAGGTGTT CATGTGTGGA
    ACCAATGCCT TTTCCCCCAT GTGCACCAGC AGACAGGTGG GGAACCTCAG CCGGACTATT
    GAGAAGATCA ATGGTGTGGC CCGCTGCCCC TATGACCCAC GCCACAACTC CACAGCTGTC
    ATCTCCTCCC AGGGGGAGCT CTATGCAGCC ACGGTCATCG ACTTCTCAGG TCGGGACCCT
    GCCATCTACC GCAGCCTGGG CAGTGGGCCA CCGCTTCGCA CTGCCCAATA TAACTCCAAG
    TGGCTTAATG AGCCAAACTT CGTGGCAGCC TATGATATTG GGCTGTTTGC ATACTTCTTC
    CTGCGGGAGA ACGCAGTGGA GCACGACTGT GGACGCACCG TGTACTCTCG CGTGGCCCGC
    GTGTGCAAGA ATGACGTGGG GGGCCGATTC CTGCTGGAGG ACACATGGAC CACATTCATG
    AAGGCCCGGC TCAACTGCTC CCGCCCGGGC GAGGTCCCCT TCTACTATAA CGAGCTGCAG
    AGTGCCTTCC ACTTGCCGGA GCAGGACCTC ATCTATGGAG TTTTCACAAC CAACGTAAAC
    AGCATCGCGG CTTCTGCTGT CTGCGCCTTC AACCTCAGTG CTATCTCCCA GGCTTTCAAT
    GGCCCATTTC GCTACCAGGA GAACCCCAGG GCTGCCTGGC TCCCCATAGC CAACCCCATC
    CCCAATTTCC AGTGTGGCAC CCTGCCTGAG ACCGGTCCCA ACGAGAACCT GACGGAGCGC
    AGCCTGCAGG ACGCGCAGCG CCTCTTCCTG ATGAGCGAGG CCGTGCAGCC GGTGACACCC
    GAGCCCTGTG TCACCCAGGA CAGCGTGCGC TTCTCACACC TCGTGGTGGA CCTGGTGCAG
    GCTAAAGACA CGCTCTACCA TGTACTCTAC ATTGGCACCG AGTCGGGCAC CATCCTGAAG
    GCGCTGTCCA CGGCGAGCCG CAGCCTCCAC GGCTGCTACC TGGAGGAGCT GCACGTGCTG
    CCCCCCGGGC GCCGCGAGCC CCTGCGCAGC CTGCGCATCC TGCACAGCGC CCGCGCGCTC
    TTCGTGGGGC TGAGAGACGG CGTCCTGCGG GTCCCACTGG AGAGGTGCGC CGCCTACCGC
    AGCCAGGGGG CATGCCTGGG GGCCCGGGAC CCGTACTGTG GCTGGGACGG GAAGCAGCAA
    CGTTGCAGCA CACTCGAGGA CAGCTCCAAC ATGAGCCTCT GGACCCAGAA CATCACCGCC
    TGTCCTGTGC GGAATGTGAC ACGGGATGGG GGCTTCGGCC CATGGTCACC ATGGCAACCA
    TGTGAGCACT TGGATGGGGA CAACTCAGGC TCTTGCCTGT GTCGAGCTCG ATCCTGTGAT
    TCCCCTCGAC CCCGCTGTGG GGGCCTTGAC TGCCTGGGGC CAGCCATCCA CATCGCCAAC
    TGCTCCAGGA ATGGGGCGTG GACCCCGTGG TCATCGTGGG CGCTGTGCAG CACGTCCTGT
    GGCATCGGCT TCCAGGTCCG CCAGCGAAGT TGCAGCAACC CTGCTCCCCG CCACGGGGGC
    CGCATCTGCG TGGGCAAGAG CCGGGAGGAA CGGTTCTGTA ATGAGAACAC GCCTTGCCCG
    GTGCCCATCT TCTGGGCTTC CTGGGGCTCC TGGAGCAAGT GCAGCAGCAA CTGTGGAGGG
    GGCATGCAGT CGCGGCGTCG GGCCTGCGAG AACGGCAACT CCTGCCTGGG CTGCGGCGTG
    GAGTTCAAGA CGTGCAACCC CGAGGGCTGC CCCGAAGTGC GGCGCAACAC CCCCTGGACG
    CCGTGGCTGC CCGTGAACGT GACGCAGGGC GGGGCACGGC AGGAGCAGCG GTTCCGCTTC
    ACCTGCCGCG CGCCCCTTGC AGACCCGCAC GGCCTGCAGT TCGGCAGGAG AAGGACCGAG
    ACGAGGACCT GTCCCGCGGA CGGCTCCGGC TCCTGCGACA CCGACGCCCT GGTGGAGGTC
    CTCCTGCGCA GCGGGAGCAC CTCCCCGCAC ACGGTGAGCG GGGGCTGGGC CGCCTGGGGC
    CCGTGGTCGT CCTGCTCCCG GGACTGCGAG CTGGGCTTCC GCGTCCGCAA GAGAACGTGC
    ACTAACCCGG AGCCCCGCAA CGGGGGCCTG CCCTGCGTGG GCGATGCTGC CGAGTACCAG
    GACTGCAACC CCCAGGCTTG CCCAGTTCGG GGTGCTTGGT CCTGCTGGAC CTCATGGTCT
    CCATGCTCAG CTTCCTGTGG TGGGGGTCAC TATCAACGCA CCCGTTCCTG CACCAGCCCC
    GCACCCTCCC CAGGTGAGGA CATCTGTCTC GGGCTGCACA CGGAGGAGGC ACTATGTGCC
    ACACAGGCCT GCCCAGAAGG CTGGTCGCCC TGGTCTGAGT GGAGTAAGTG CACTGACGAC
    GGAGCCCAGA GCCGAAGCCG GCACTGTGAG GAGCTCCTCC CAGGGTCCAG CGCCTGTGCT
    GGAAACAGCA GCCAGAGCCG CCCCTGCCCC TACAGCGAGA TTCCCGTCAT CCTGCCAGCC
    TCCAGCATGG AGGAGGCCAC CGACTGTGCA GGGTTCAATC TCATCCACTT GGTGGCCACG
    GGCATCTCCT GCTTCTTGGG CTCTGGGCTC CTGACCCTAG CAGTGTACCT GTCTTGCCAG
    CACTGCCAGC GTCAGTCCCA GGAGTCCACA CTGGTCCATC CTGCCACCCC CAACCATTTG
    CACTACAAGG GCGGAGGCAC CCCGAAGAAT GAAAAGTACA CACCCATGGA ATTCAAGACC
    CTGAACAAGA ATAACTTGAT CCCTGATGAC AGAGCCAACT TCTACCCATT GCAGCAGACC
    AATGTGTACA CGACTACTTA CTACCCAAGC CCCCTGAACA AACACAGCTT CCGGCCCGAG
    GCCTCACCTG GACAACGGTG CTTCCCCAAC AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.