POLR2I cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR2I
Entrez Gene ID	5438
Full Name	RNA polymerase II subunit I
Synonyms	RPB9,hRPB14.5
General protein information	Preferred Names RNA polymerase II subunit I Names DNA-directed RNA polymerase II subunit RPB9 DNA-directed RNA polymerase II 14.5 kDa polypeptide DNA-directed RNA polymerase II subunit I RNA polymerase II 14.5 kDa subunit RNA polymerase II subunit B9 RPB14.5 polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa polymerase (RNA) II subunit I
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.12
Summary	This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq, Jul 2008].
Disorder MIM:	180662

mRNA and Protein(s)

mRNA	Protein	Name
NM_006233.4	NP_006224.1	DNA-directed RNA polymerase II subunit RPB9
NM_006233.5	NP_006224.1	DNA-directed RNA polymerase II subunit RPB9

Pathways from BioSystems

Homologies

Bos taurus (cattle)	POLR2I	NP_001069797.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008393	XP_317057.2
Gallus gallus (chicken)	POLR2I	XP_423952.3
Danio rerio (zebrafish)	polr2i	NP_001006013.2
Drosophila melanogaster (fruit fly)	RpII15	NP_001247099.1
Saccharomyces cerevisiae (baker's yeast)	RPB9	NP_011445.1
Schizosaccharomyces pombe (fission yeast)	rpb9	NP_594705.1
Oryza sativa (rice)	Os12g0560400	NP_001067017.1
Pan troglodytes (chimpanzee)	POLR2I	XP_001162834.1
Mus musculus (house mouse)	Polr2i	NP_081535.1
Caenorhabditis elegans (roundworm)	rpb-9	NP_505062.1
Kluyveromyces lactis	KLLA0D03234g	XP_453210.1
Xenopus tropicalis (tropical clawed frog)	polr2i	XP_002932661.1
Homo sapiens (human)	POLR2I	NP_006224.1
Canis lupus familiaris (dog)	POLR2I	XP_853642.1
Eremothecium gossypii	AGOS_AFL088W	NP_985460.1
	POLR2I	XP_001102318.1
Magnaporthe oryzae (rice blast fungus)	MGG_07391	XP_003711269.1

Gene Ontology
Bibliography

Related articles in PubMed

The DNA sequence and biology of human chromosome 19.
Grimwood J, Gordon LA, Olsen A, Terry A, Schmutz J, Lamerdin J, Hellsten U, Goodstein D, Couronne O, Tran-Gyamfi M, Aerts A, Altherr M, Ashworth L, Bajorek E, Black S, Branscomb E, Caenepeel S, Carrano A, Caoile C, Chan YM, Christensen M, Cleland CA, Copeland A, Dalin E, Dehal P, Denys M, Detter JC, Escobar J, Flowers D, Fotopulos D, Garcia C, Georgescu AM, Glavina T, Gomez M, Gonzales E, Groza M, Hammon N, Hawkins T, Haydu L, Ho I, Huang W, Israni S, Jett J, Kadner K, Kimball H, Kobayashi A, Larionov V, Leem SH, Lopez F, Lou Y, Lowry S, Malfatti S, Martinez D, McCready P, Medina C, Morgan J, Nelson K, Nolan M, Ovcharenko I, Pitluck S, Pollard M, Popkie AP, Predki P, Quan G, Ramirez L, Rash S, Retterer J, Rodriguez A, Rogers S, Salamov A, Salazar A, She X, Smith D, Slezak T, Solovyev V, Thayer N, Tice H, Tsai M, Ustaszewska A, Vo N, Wagner M, Wheeler J, Wu K, Xie G, Yang J, Dubchak I, Furey TS, DeJong P, Dickson M, Gordon D, Eichler EE, Pennacchio LA, Richardson P, Stubbs L, Rokhsar DS, Myers RM, Rubin EM, Lucas SM
Nature428(6982)529-35(2004 Apr)

The Tat/TAR-dependent phosphorylation of RNA polymerase II C-terminal domain stimulates cotranscriptional capping of HIV-1 mRNA.
Zhou M, Deng L, Kashanchi F, Brady JN, Shatkin AJ, Kumar A
Proceedings of the National Academy of Sciences of the United States of America100(22)12666-71(2003 Oct)

Acetylation of Tat defines a cyclinT1-independent step in HIV transactivation.
Kaehlcke K, Dorr A, Hetzer-Egger C, Kiermer V, Henklein P, Schnoelzer M, Loret E, Cole PA, Verdin E, Ott M
Molecular cell12(1)167-76(2003 Jul)

The RNA polymerase II elongation complex.
Shilatifard A, Conaway RC, Conaway JW
Annual review of biochemistry72693-715(2003)

HIV-1 Vpr binding to HIV-1 LTR C/EBP cis-acting elements and adjacent regions is sequence-specific.
Hogan TH, Nonnemacher MR, Krebs FC, Henderson A, Wigdahl B
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie57(1)41-8(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLR2I gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR2I cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05463	NM_006233.5 Latest version!	Homo sapiens RNA polymerase II subunit I (POLR2I), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu05463	NM_006233.4	Homo sapiens RNA polymerase II subunit I (POLR2I), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR2I ( NM_006233.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006224.1 Homo sapiens RNA polymerase II subunit I (POLR2I), mRNA.

Price & Availability↑

CloneID	OHu05463
Clone ID Related Accession (Same CDS sequence)	NM_006233.4 , NM_006233.5
Accession Version	NM_006233.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC067794.1 and BC017112.1. On Jun 2, 2004 this sequence version replaced NM_006233.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC067794.1, SRR1163655.463084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467144 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGCCCG ACGGGACTTA CGAGCCGGGC TTCGTGGGTA TTCGCTTCTG CCAGGAATGT 
AACAACATGC TGTACCCCAA GGAAGACAAG GAGAACCGCA TTCTGCTCTA CGCGTGCCGG 
AACTGTGATT ACCAGCAGGA GGCCGACAAC AGCTGCATCT ATGTCAACAA GATCACGCAC 
GAAGTGGACG AACTGACCCA GATTATCGCC GACGTGTCCC AGGACCCCAC GTTGCCGCGG 
ACCGAGGACC ACCCGTGCCA AAAGTGCGGC CACAAGGAGG CTGTGTTCTT CCAGTCACAC 
AGTGCGCGGG CCGAGGACGC CATGCGCCTT TACTACGTGT GCACAGCCCC ACACTGCGGC 
CACCGCTGGA CCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006224.1
CDS	449..826
Translation	MEPDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE

Target ORF information:

RefSeq Version	NM_006233.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit I (POLR2I), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006233.4

ORF Insert Sequence:

ATGGAGCCCG ACGGGACTTA CGAGCCGGGC TTCGTGGGTA TTCGCTTCTG CCAGGAATGT 
AACAACATGC TGTACCCCAA GGAAGACAAG GAGAACCGCA TTCTGCTCTA CGCGTGCCGG 
AACTGTGATT ACCAGCAGGA GGCCGACAAC AGCTGCATCT ATGTCAACAA GATCACGCAC 
GAAGTGGACG AACTGACCCA GATTATCGCC GACGTGTCCC AGGACCCCAC GTTGCCGCGG 
ACCGAGGACC ACCCGTGCCA AAAGTGCGGC CACAAGGAGG CTGTGTTCTT CCAGTCACAC 
AGTGCGCGGG CCGAGGACGC CATGCGCCTT TACTACGTGT GCACAGCCCC ACACTGCGGC 
CACCGCTGGA CCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR2I ( NM_006233.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_006224.1 Homo sapiens RNA polymerase II subunit I (POLR2I), mRNA.

Price & Availability↑

CloneID	OHu05463
Clone ID Related Accession (Same CDS sequence)	NM_006233.4 , NM_006233.5
Accession Version	NM_006233.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	DNA-directed RNA polymerase II subunit RPB9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC017112.1. On Nov 22, 2018 this sequence version replaced NM_006233.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017112.1, SRR1163655.549868.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467144 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000221859.9/ ENSP00000221859.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCCCG ACGGGACTTA CGAGCCGGGC TTCGTGGGTA TTCGCTTCTG CCAGGAATGT 
AACAACATGC TGTACCCCAA GGAAGACAAG GAGAACCGCA TTCTGCTCTA CGCGTGCCGG 
AACTGTGATT ACCAGCAGGA GGCCGACAAC AGCTGCATCT ATGTCAACAA GATCACGCAC 
GAAGTGGACG AACTGACCCA GATTATCGCC GACGTGTCCC AGGACCCCAC GTTGCCGCGG 
ACCGAGGACC ACCCGTGCCA AAAGTGCGGC CACAAGGAGG CTGTGTTCTT CCAGTCACAC 
AGTGCGCGGG CCGAGGACGC CATGCGCCTT TACTACGTGT GCACAGCCCC ACACTGCGGC 
CACCGCTGGA CCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006224.1
CDS	20..397
Translation	MEPDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE

Target ORF information:

RefSeq Version	NM_006233.5
Organism	Homo sapiens(human)
Definition	Homo sapiens RNA polymerase II subunit I (POLR2I), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006233.5

ORF Insert Sequence:

ATGGAGCCCG ACGGGACTTA CGAGCCGGGC TTCGTGGGTA TTCGCTTCTG CCAGGAATGT 
AACAACATGC TGTACCCCAA GGAAGACAAG GAGAACCGCA TTCTGCTCTA CGCGTGCCGG 
AACTGTGATT ACCAGCAGGA GGCCGACAAC AGCTGCATCT ATGTCAACAA GATCACGCAC 
GAAGTGGACG AACTGACCCA GATTATCGCC GACGTGTCCC AGGACCCCAC GTTGCCGCGG 
ACCGAGGACC ACCCGTGCCA AAAGTGCGGC CACAAGGAGG CTGTGTTCTT CCAGTCACAC 
AGTGCGCGGG CCGAGGACGC CATGCGCCTT TACTACGTGT GCACAGCCCC ACACTGCGGC 
CACCGCTGGA CCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The DNA sequence and biology of human chromosome 19.
Nature428(6982)529-35(2004 Apr)
Grimwood J,Gordon LA,Olsen A,Terry A,Schmutz J,Lamerdin J,Hellsten U,Goodstein D,Couronne O,Tran-Gyamfi M,Aerts A,Altherr M,Ashworth L,Bajorek E,Black S,Branscomb E,Caenepeel S,Carrano A,Caoile C,Chan YM,Christensen M,Cleland CA,Copeland A,Dalin E,Dehal P,Denys M,Detter JC,Escobar J,Flowers D,Fotopulos D,Garcia C,Georgescu AM,Glavina T,Gomez M,Gonzales E,Groza M,Hammon N,Hawkins T,Haydu L,Ho I,Huang W,Israni S,Jett J,Kadner K,Kimball H,Kobayashi A,Larionov V,Leem SH,Lopez F,Lou Y,Lowry S,Malfatti S,Martinez D,McCready P,Medina C,Morgan J,Nelson K,Nolan M,Ovcharenko I,Pitluck S,Pollard M,Popkie AP,Predki P,Quan G,Ramirez L,Rash S,Retterer J,Rodriguez A,Rogers S,Salamov A,Salazar A,She X,Smith D,Slezak T,Solovyev V,Thayer N,Tice H,Tsai M,Ustaszewska A,Vo N,Wagner M,Wheeler J,Wu K,Xie G,Yang J,Dubchak I,Furey TS,DeJong P,Dickson M,Gordon D,Eichler EE,Pennacchio LA,Richardson P,Stubbs L,Rokhsar DS,Myers RM,Rubin EM,Lucas SM

The Tat/TAR-dependent phosphorylation of RNA polymerase II C-terminal domain stimulates cotranscriptional capping of HIV-1 mRNA.
Proceedings of the National Academy of Sciences of the United States of America100(22)12666-71(2003 Oct)
Zhou M,Deng L,Kashanchi F,Brady JN,Shatkin AJ,Kumar A

Acetylation of Tat defines a cyclinT1-independent step in HIV transactivation.
Molecular cell12(1)167-76(2003 Jul)
Kaehlcke K,Dorr A,Hetzer-Egger C,Kiermer V,Henklein P,Schnoelzer M,Loret E,Cole PA,Verdin E,Ott M

The RNA polymerase II elongation complex.
Annual review of biochemistry72693-715(2003)
Shilatifard A,Conaway RC,Conaway JW

HIV-1 Vpr binding to HIV-1 LTR C/EBP cis-acting elements and adjacent regions is sequence-specific.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie57(1)41-8(2003 Jan)
Hogan TH,Nonnemacher MR,Krebs FC,Henderson A,Wigdahl B

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POLR2I cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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