POLA1 cDNA ORF clone, Homo sapiens(human)

The following POLA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu59806 XM_011545541.2
Latest version!
Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu19436 NM_016937.4
Latest version!
Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OHu53935 XM_006724499.2
Latest version!
Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $643.30
$919.00
OHu106266 XM_024452392.1
Latest version!
Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu99340 XM_017029594.2
Latest version!
Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu99341 XM_017029595.2
Latest version!
Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu99342 XM_017029596.1
Latest version!
Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu53934 NM_001330360.2
Latest version!
Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu59805 XM_011545540.3
Latest version!
Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu59805 XM_011545540.2 Homo sapiens polymerase (DNA) alpha 1, catalytic subunit (POLA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu53934 NM_001330360.1 Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu99340 XM_017029594.1 Homo sapiens polymerase (DNA) alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu19436 NM_016937.3 Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OHu99341 XM_017029595.1 Homo sapiens polymerase (DNA) alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu59806
    Clone ID Related Accession (Same CDS sequence) XM_011545541.2
    Accession Version XM_011545541.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545541.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGATG GAGAACAAGA
    ACAGATCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543843.1
    CDS86..2677
    Translation

    Target ORF information:

    RefSeq Version XM_011545541.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545541.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGATG GAGAACAAGA
    ACAGATCCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19436
    Clone ID Related Accession (Same CDS sequence) NM_016937.3 , NM_016937.4
    Accession Version NM_016937.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X06745.1, BX648513.1, BU508486.1, CN364591.1 and BM664024.1. This sequence is a reference standard in the RefSeqGene project. On Mar 13, 2010 this sequence version replaced NM_016937.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X06745.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCACCTG TGCACGGCGA CGACTCTCTG TCAGATTCAG GGAGTTTTGT ATCTTCTCGA 
    GCCCGGCGAG AAAAAAAATC AAAGAAGGGG CGCCAAGAAG CCCTAGAAAG ACTGAAAAAG
    GCTAAAGCTG GTGAGAAGTA TAAATATGAA GTCGAGGACT TCACAGGTGT TTATGAAGAA
    GTTGATGAAG AACAGTATTC GAAGCTGGTT CAGGCACGCC AGGATGATGA CTGGATTGTG
    GATGATGATG GTATTGGCTA TGTGGAAGAT GGCCGAGAGA TTTTTGATGA TGACCTTGAA
    GATGATGCCC TTGATGCTGA TGAGAAAGGA AAAGATGGTA AAGCACGCAA TAAAGACAAG
    AGGAATGTAA AGAAGCTCGC AGTGACAAAA CCGAACAACA TTAAGTCAAT GTTCATTGCT
    TGTGCTGGAA AGAAAACTGC AGATAAAGCT GTAGACTTGT CCAAGGATGG TCTGCTAGGT
    GACATTCTAC AGGATCTTAA CACTGAGACA CCTCAAATAA CTCCACCACC TGTAATGATA
    CTGAAGAAGA AAAGATCCAT TGGAGCTTCA CCGAATCCTT TCTCTGTGCA CACCGCCACG
    GCAGTTCCTT CAGGAAAAAT TGCTTCCCCT GTCTCCAGAA AGGAGCCTCC ATTAACTCCT
    GTTCCTCTTA AACGTGCTGA ATTTGCTGGC GATGATGTAC AGGTCGAGAG TACAGAAGAA
    GAGCAGGAGT CAGGGGCAAT GGAGTTTGAA GATGGTGACT TTGATGAGCC CATGGAAGTT
    GAAGAGGTGG ACCTGGAGCC TATGGCTGCC AAGGCTTGGG ACAAAGAGAG TGAGCCAGCA
    GAGGAAGTGA AACAAGAGGC GGATTCTGGG AAAGGGACCG TGTCCTACTT AGGAAGTTTT
    CTCCCGGATG TCTCTTGTTG GGACATTGAT CAAGAAGGTG ATAGCAGTTT CTCAGTGCAA
    GAAGTTCAAG TGGATTCCAG TCACCTCCCA TTGGTAAAAG GGGCAGATGA GGAACAAGTA
    TTCCACTTTT ATTGGTTGGA TGCTTATGAG GATCAGTACA ACCAACCAGG TGTGGTATTT
    CTGTTTGGGA AAGTTTGGAT TGAATCAGCC GAGACCCATG TGAGCTGTTG TGTCATGGTG
    AAAAATATCG AGCGAACGCT TTACTTCCTT CCCCGTGAAA TGAAAATTGA TCTAAATACG
    GGGAAAGAAA CAGGAACTCC AATTTCAATG AAGGATGTTT ATGAGGAATT TGATGAGAAA
    ATAGCAACAA AATATAAAAT TATGAAGTTC AAGTCTAAGC CAGTGGAAAA GAACTATGCT
    TTTGAGATAC CTGATGTTCC AGAAAAATCT GAGTACTTGG AAGTTAAATA CTCGGCTGAA
    ATGCCACAGC TTCCTCAAGA TTTGAAAGGA GAAACTTTTT CTCATGTATT TGGGACCAAC
    ACATCTAGCC TGGAACTGTT CTTGATGAAC AGAAAGATCA AAGGACCTTG TTGGCTTGAA
    GTAAAAAGTC CACAGCTCTT GAATCAGCCA GTCAGTTGGT GTAAAGTTGA GGCAATGGCT
    TTGAAACCAG ACCTGGTGAA TGTAATTAAG GATGTCAGTC CACCACCGCT TGTCGTGATG
    GCTTTCAGCA TGAAGACAAT GCAGAATGCA AAGAACCATC AAAATGAGAT TATTGCTATG
    GCAGCTTTGG TCCATCACAG TTTTGCATTG GATAAAGCAG CCCCAAAGCC TCCCTTTCAG
    TCACACTTCT GTGTTGTGTC TAAACCAAAG GACTGTATTT TTCCATATGC TTTCAAAGAA
    GTCATTGAGA AAAAGAATGT GAAGGTTGAG GTTGCTGCAA CAGAAAGAAC ACTGCTAGGT
    TTTTTCCTTG CAAAAGTTCA CAAAATTGAT CCTGATATCA TTGTGGGTCA TAATATTTAT
    GGGTTTGAAC TGGAAGTACT ACTGCAGAGA ATTAATGTGT GCAAAGCTCC TCACTGGTCC
    AAGATAGGTC GACTGAAGCG ATCCAACATG CCAAAGCTTG GGGGCCGGAG TGGATTTGGT
    GAAAGAAATG CTACCTGTGG TCGAATGATC TGTGATGTGG AAATTTCAGC AAAGGAATTG
    ATTCGTTGTA AAAGCTACCA TCTGTCTGAA CTTGTTCAGC AGATTCTAAA AACTGAAAGG
    GTTGTAATCC CAATGGAAAA TATACAAAAT ATGTACAGTG AATCTTCTCA ACTGTTATAC
    CTGTTGGAAC ACACCTGGAA AGATGCCAAG TTCATTTTGC AGATCATGTG TGAGCTAAAT
    GTTCTTCCAT TAGCATTGCA GATCACTAAC ATCGCTGGGA ACATTATGTC CAGGACGCTG
    ATGGGTGGAC GATCCGAGCG TAACGAGTTC TTGTTGCTTC ATGCATTTTA CGAAAACAAC
    TATATTGTGC CTGACAAGCA GATTTTCAGA AAGCCTCAGC AAAAACTGGG AGATGAAGAT
    GAAGAAATTG ATGGAGATAC CAATAAATAC AAGAAAGGAC GTAAGAAAGC AGCTTATGCT
    GGAGGCTTGG TTTTGGACCC CAAAGTTGGT TTTTATGATA AGTTCATTTT GCTTCTGGAC
    TTCAACAGTC TATATCCTTC CATCATTCAG GAATTTAACA TTTGTTTTAC AACAGTACAA
    AGAGTTGCTT CAGAGGCACA GAAAGTTACA GAGGATGGAG AACAAGAACA GATCCCTGAG
    TTGCCAGATC CAAGCTTAGA AATGGGCATT TTGCCCAGAG AGATCCGGAA ACTGGTAGAA
    CGGAGAAAAC AAGTCAAACA GCTAATGAAA CAGCAAGACT TAAATCCAGA CCTTATTCTT
    CAGTATGACA TTCGACAGAA GGCTTTGAAG CTCACAGCGA ACAGTATGTA TGGTTGCCTG
    GGATTTTCCT ATAGCAGATT TTACGCCAAA CCACTGGCTG CCTTGGTGAC ATACAAAGGA
    AGGGAGATTT TGATGCATAC GAAAGAGATG GTACAAAAGA TGAATCTTGA AGTTATTTAT
    GGAGATACAG ATTCAATTAT GATAAACACC AATAGCACCA ATCTGGAAGA AGTATTTAAG
    TTGGGAAACA AGGTAAAAAG TGAAGTGAAT AAGTTGTACA AACTGCTTGA AATAGACATT
    GATGGGGTTT TCAAGTCTCT GCTACTGCTG AAAAAAAAGA AGTACGCTGC TCTGGTTGTT
    GAGCCAACGT CGGATGGGAA TTATGTCACC AAACAGGAGC TCAAAGGATT AGATATAGTT
    AGAAGAGATT GGTGTGATCT TGCTAAAGAC ACTGGAAACT TTGTGATTGG CCAGATTCTT
    TCTGATCAAA GCCGGGACAC TATAGTGGAA AACATTCAGA AGAGGCTGAT AGAAATTGGA
    GAAAATGTGC TAAATGGCAG TGTCCCAGTG AGCCAGTTTG AAATTAACAA GGCATTGACA
    AAGGATCCCC AGGATTACCC TGATAAAAAA AGCCTACCTC ATGTACATGT TGCCCTCTGG
    ATAAATTCTC AAGGAGGCAG AAAGGTGAAA GCTGGAGATA CTGTGTCATA TGTCATCTGT
    CAGGATGGAT CAAACCTCAC TGCAAGTCAG AGGGCCTATG CGCCTGAGCA GCTGCAGAAA
    CAGGATAATC TAACCATTGA CACCCAGTAC TACCTGGCCC AGCAGATCCA CCCAGTCGTG
    GCTCGGATCT GTGAACCAAT AGACGGAATT GATGCTGTCC TCATTGCAAC GTGGTTGGGA
    CTTGACCCCA CCCAATTTAG AGTTCATCAT TATCATAAAG ATGAAGAGAA TGATGCTCTA
    CTTGGTGGCC CAGCACAGCT CACTGATGAA GAGAAATACA GGGACTGTGA AAGATTCAAA
    TGTCCATGCC CTACATGTGG AACTGAGAAT ATTTATGATA ATGTCTTTGA TGGTTCGGGA
    ACAGATATGG AGCCCAGCTT GTATCGTTGC AGTAACATCG ATTGTAAGGC TTCACCTCTG
    ACCTTTACAG TACAACTGAG CAACAAATTG ATCATGGACA TTAGACGTTT CATTAAAAAG
    TACTATGATG GCTGGTTGAT ATGTGAAGAG CCAACCTGTC GCAATCGAAC TCGTCACCTT
    CCCCTTCAAT TCTCCCGAAC TGGGCCTCTT TGCCCAGCCT GCATGAAAGC TACACTTCAA
    CCAGAGTATT CTGACAAGTC CCTGTACACC CAGCTGTGCT TTTACCGGTA CATTTTTGAT
    GCGGAGTGTG CACTGGAGAA ACTTACTACC GATCATGAGA AAGATAAATT GAAGAAGCAA
    TTTTTTACCC CCAAAGTTCT GCAGGACTAC AGAAAACTCA AGAACACAGC AGAGCAATTC
    TTGTCCCGAA GTGGCTACTC CGAAGTGAAT CTGAGCAAAC TCTTCGCTGG TTGTGCCGTG
    AAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_058633.2
    CDS16..4404
    Translation

    Target ORF information:

    RefSeq Version NM_016937.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_016937.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCACCTG TGCACGGCGA CGACTCTCTG TCAGATTCAG GGAGTTTTGT ATCTTCTCGA 
    GCCCGGCGAG AAAAAAAATC AAAGAAGGGG CGCCAAGAAG CCCTAGAAAG ACTGAAAAAG
    GCTAAAGCTG GTGAGAAGTA TAAATATGAA GTCGAGGACT TCACAGGTGT TTATGAAGAA
    GTTGATGAAG AACAGTATTC GAAGCTGGTT CAGGCACGCC AGGATGATGA CTGGATTGTG
    GATGATGATG GTATTGGCTA TGTGGAAGAT GGCCGAGAGA TTTTTGATGA TGACCTTGAA
    GATGATGCCC TTGATGCTGA TGAGAAAGGA AAAGATGGTA AAGCACGCAA TAAAGACAAG
    AGGAATGTAA AGAAGCTCGC AGTGACAAAA CCGAACAACA TTAAGTCAAT GTTCATTGCT
    TGTGCTGGAA AGAAAACTGC AGATAAAGCT GTAGACTTGT CCAAGGATGG TCTGCTAGGT
    GACATTCTAC AGGATCTTAA CACTGAGACA CCTCAAATAA CTCCACCACC TGTAATGATA
    CTGAAGAAGA AAAGATCCAT TGGAGCTTCA CCGAATCCTT TCTCTGTGCA CACCGCCACG
    GCAGTTCCTT CAGGAAAAAT TGCTTCCCCT GTCTCCAGAA AGGAGCCTCC ATTAACTCCT
    GTTCCTCTTA AACGTGCTGA ATTTGCTGGC GATGATGTAC AGGTCGAGAG TACAGAAGAA
    GAGCAGGAGT CAGGGGCAAT GGAGTTTGAA GATGGTGACT TTGATGAGCC CATGGAAGTT
    GAAGAGGTGG ACCTGGAGCC TATGGCTGCC AAGGCTTGGG ACAAAGAGAG TGAGCCAGCA
    GAGGAAGTGA AACAAGAGGC GGATTCTGGG AAAGGGACCG TGTCCTACTT AGGAAGTTTT
    CTCCCGGATG TCTCTTGTTG GGACATTGAT CAAGAAGGTG ATAGCAGTTT CTCAGTGCAA
    GAAGTTCAAG TGGATTCCAG TCACCTCCCA TTGGTAAAAG GGGCAGATGA GGAACAAGTA
    TTCCACTTTT ATTGGTTGGA TGCTTATGAG GATCAGTACA ACCAACCAGG TGTGGTATTT
    CTGTTTGGGA AAGTTTGGAT TGAATCAGCC GAGACCCATG TGAGCTGTTG TGTCATGGTG
    AAAAATATCG AGCGAACGCT TTACTTCCTT CCCCGTGAAA TGAAAATTGA TCTAAATACG
    GGGAAAGAAA CAGGAACTCC AATTTCAATG AAGGATGTTT ATGAGGAATT TGATGAGAAA
    ATAGCAACAA AATATAAAAT TATGAAGTTC AAGTCTAAGC CAGTGGAAAA GAACTATGCT
    TTTGAGATAC CTGATGTTCC AGAAAAATCT GAGTACTTGG AAGTTAAATA CTCGGCTGAA
    ATGCCACAGC TTCCTCAAGA TTTGAAAGGA GAAACTTTTT CTCATGTATT TGGGACCAAC
    ACATCTAGCC TGGAACTGTT CTTGATGAAC AGAAAGATCA AAGGACCTTG TTGGCTTGAA
    GTAAAAAGTC CACAGCTCTT GAATCAGCCA GTCAGTTGGT GTAAAGTTGA GGCAATGGCT
    TTGAAACCAG ACCTGGTGAA TGTAATTAAG GATGTCAGTC CACCACCGCT TGTCGTGATG
    GCTTTCAGCA TGAAGACAAT GCAGAATGCA AAGAACCATC AAAATGAGAT TATTGCTATG
    GCAGCTTTGG TCCATCACAG TTTTGCATTG GATAAAGCAG CCCCAAAGCC TCCCTTTCAG
    TCACACTTCT GTGTTGTGTC TAAACCAAAG GACTGTATTT TTCCATATGC TTTCAAAGAA
    GTCATTGAGA AAAAGAATGT GAAGGTTGAG GTTGCTGCAA CAGAAAGAAC ACTGCTAGGT
    TTTTTCCTTG CAAAAGTTCA CAAAATTGAT CCTGATATCA TTGTGGGTCA TAATATTTAT
    GGGTTTGAAC TGGAAGTACT ACTGCAGAGA ATTAATGTGT GCAAAGCTCC TCACTGGTCC
    AAGATAGGTC GACTGAAGCG ATCCAACATG CCAAAGCTTG GGGGCCGGAG TGGATTTGGT
    GAAAGAAATG CTACCTGTGG TCGAATGATC TGTGATGTGG AAATTTCAGC AAAGGAATTG
    ATTCGTTGTA AAAGCTACCA TCTGTCTGAA CTTGTTCAGC AGATTCTAAA AACTGAAAGG
    GTTGTAATCC CAATGGAAAA TATACAAAAT ATGTACAGTG AATCTTCTCA ACTGTTATAC
    CTGTTGGAAC ACACCTGGAA AGATGCCAAG TTCATTTTGC AGATCATGTG TGAGCTAAAT
    GTTCTTCCAT TAGCATTGCA GATCACTAAC ATCGCTGGGA ACATTATGTC CAGGACGCTG
    ATGGGTGGAC GATCCGAGCG TAACGAGTTC TTGTTGCTTC ATGCATTTTA CGAAAACAAC
    TATATTGTGC CTGACAAGCA GATTTTCAGA AAGCCTCAGC AAAAACTGGG AGATGAAGAT
    GAAGAAATTG ATGGAGATAC CAATAAATAC AAGAAAGGAC GTAAGAAAGC AGCTTATGCT
    GGAGGCTTGG TTTTGGACCC CAAAGTTGGT TTTTATGATA AGTTCATTTT GCTTCTGGAC
    TTCAACAGTC TATATCCTTC CATCATTCAG GAATTTAACA TTTGTTTTAC AACAGTACAA
    AGAGTTGCTT CAGAGGCACA GAAAGTTACA GAGGATGGAG AACAAGAACA GATCCCTGAG
    TTGCCAGATC CAAGCTTAGA AATGGGCATT TTGCCCAGAG AGATCCGGAA ACTGGTAGAA
    CGGAGAAAAC AAGTCAAACA GCTAATGAAA CAGCAAGACT TAAATCCAGA CCTTATTCTT
    CAGTATGACA TTCGACAGAA GGCTTTGAAG CTCACAGCGA ACAGTATGTA TGGTTGCCTG
    GGATTTTCCT ATAGCAGATT TTACGCCAAA CCACTGGCTG CCTTGGTGAC ATACAAAGGA
    AGGGAGATTT TGATGCATAC GAAAGAGATG GTACAAAAGA TGAATCTTGA AGTTATTTAT
    GGAGATACAG ATTCAATTAT GATAAACACC AATAGCACCA ATCTGGAAGA AGTATTTAAG
    TTGGGAAACA AGGTAAAAAG TGAAGTGAAT AAGTTGTACA AACTGCTTGA AATAGACATT
    GATGGGGTTT TCAAGTCTCT GCTACTGCTG AAAAAAAAGA AGTACGCTGC TCTGGTTGTT
    GAGCCAACGT CGGATGGGAA TTATGTCACC AAACAGGAGC TCAAAGGATT AGATATAGTT
    AGAAGAGATT GGTGTGATCT TGCTAAAGAC ACTGGAAACT TTGTGATTGG CCAGATTCTT
    TCTGATCAAA GCCGGGACAC TATAGTGGAA AACATTCAGA AGAGGCTGAT AGAAATTGGA
    GAAAATGTGC TAAATGGCAG TGTCCCAGTG AGCCAGTTTG AAATTAACAA GGCATTGACA
    AAGGATCCCC AGGATTACCC TGATAAAAAA AGCCTACCTC ATGTACATGT TGCCCTCTGG
    ATAAATTCTC AAGGAGGCAG AAAGGTGAAA GCTGGAGATA CTGTGTCATA TGTCATCTGT
    CAGGATGGAT CAAACCTCAC TGCAAGTCAG AGGGCCTATG CGCCTGAGCA GCTGCAGAAA
    CAGGATAATC TAACCATTGA CACCCAGTAC TACCTGGCCC AGCAGATCCA CCCAGTCGTG
    GCTCGGATCT GTGAACCAAT AGACGGAATT GATGCTGTCC TCATTGCAAC GTGGTTGGGA
    CTTGACCCCA CCCAATTTAG AGTTCATCAT TATCATAAAG ATGAAGAGAA TGATGCTCTA
    CTTGGTGGCC CAGCACAGCT CACTGATGAA GAGAAATACA GGGACTGTGA AAGATTCAAA
    TGTCCATGCC CTACATGTGG AACTGAGAAT ATTTATGATA ATGTCTTTGA TGGTTCGGGA
    ACAGATATGG AGCCCAGCTT GTATCGTTGC AGTAACATCG ATTGTAAGGC TTCACCTCTG
    ACCTTTACAG TACAACTGAG CAACAAATTG ATCATGGACA TTAGACGTTT CATTAAAAAG
    TACTATGATG GCTGGTTGAT ATGTGAAGAG CCAACCTGTC GCAATCGAAC TCGTCACCTT
    CCCCTTCAAT TCTCCCGAAC TGGGCCTCTT TGCCCAGCCT GCATGAAAGC TACACTTCAA
    CCAGAGTATT CTGACAAGTC CCTGTACACC CAGCTGTGCT TTTACCGGTA CATTTTTGAT
    GCGGAGTGTG CACTGGAGAA ACTTACTACC GATCATGAGA AAGATAAATT GAAGAAGCAA
    TTTTTTACCC CCAAAGTTCT GCAGGACTAC AGAAAACTCA AGAACACAGC AGAGCAATTC
    TTGTCCCGAA GTGGCTACTC CGAAGTGAAT CTGAGCAAAC TCTTCGCTGG TTGTGCCGTG
    AAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19436
    Clone ID Related Accession (Same CDS sequence) NM_016937.3 , NM_016937.4
    Accession Version NM_016937.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4389bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004655.1, X06745.1, BX648513.1, BU508486.1, CN364591.1 and BM664024.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_016937.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X06745.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCACCTG TGCACGGCGA CGACTCTCTG TCAGATTCAG GGAGTTTTGT ATCTTCTCGA 
    GCCCGGCGAG AAAAAAAATC AAAGAAGGGG CGCCAAGAAG CCCTAGAAAG ACTGAAAAAG
    GCTAAAGCTG GTGAGAAGTA TAAATATGAA GTCGAGGACT TCACAGGTGT TTATGAAGAA
    GTTGATGAAG AACAGTATTC GAAGCTGGTT CAGGCACGCC AGGATGATGA CTGGATTGTG
    GATGATGATG GTATTGGCTA TGTGGAAGAT GGCCGAGAGA TTTTTGATGA TGACCTTGAA
    GATGATGCCC TTGATGCTGA TGAGAAAGGA AAAGATGGTA AAGCACGCAA TAAAGACAAG
    AGGAATGTAA AGAAGCTCGC AGTGACAAAA CCGAACAACA TTAAGTCAAT GTTCATTGCT
    TGTGCTGGAA AGAAAACTGC AGATAAAGCT GTAGACTTGT CCAAGGATGG TCTGCTAGGT
    GACATTCTAC AGGATCTTAA CACTGAGACA CCTCAAATAA CTCCACCACC TGTAATGATA
    CTGAAGAAGA AAAGATCCAT TGGAGCTTCA CCGAATCCTT TCTCTGTGCA CACCGCCACG
    GCAGTTCCTT CAGGAAAAAT TGCTTCCCCT GTCTCCAGAA AGGAGCCTCC ATTAACTCCT
    GTTCCTCTTA AACGTGCTGA ATTTGCTGGC GATGATGTAC AGGTCGAGAG TACAGAAGAA
    GAGCAGGAGT CAGGGGCAAT GGAGTTTGAA GATGGTGACT TTGATGAGCC CATGGAAGTT
    GAAGAGGTGG ACCTGGAGCC TATGGCTGCC AAGGCTTGGG ACAAAGAGAG TGAGCCAGCA
    GAGGAAGTGA AACAAGAGGC GGATTCTGGG AAAGGGACCG TGTCCTACTT AGGAAGTTTT
    CTCCCGGATG TCTCTTGTTG GGACATTGAT CAAGAAGGTG ATAGCAGTTT CTCAGTGCAA
    GAAGTTCAAG TGGATTCCAG TCACCTCCCA TTGGTAAAAG GGGCAGATGA GGAACAAGTA
    TTCCACTTTT ATTGGTTGGA TGCTTATGAG GATCAGTACA ACCAACCAGG TGTGGTATTT
    CTGTTTGGGA AAGTTTGGAT TGAATCAGCC GAGACCCATG TGAGCTGTTG TGTCATGGTG
    AAAAATATCG AGCGAACGCT TTACTTCCTT CCCCGTGAAA TGAAAATTGA TCTAAATACG
    GGGAAAGAAA CAGGAACTCC AATTTCAATG AAGGATGTTT ATGAGGAATT TGATGAGAAA
    ATAGCAACAA AATATAAAAT TATGAAGTTC AAGTCTAAGC CAGTGGAAAA GAACTATGCT
    TTTGAGATAC CTGATGTTCC AGAAAAATCT GAGTACTTGG AAGTTAAATA CTCGGCTGAA
    ATGCCACAGC TTCCTCAAGA TTTGAAAGGA GAAACTTTTT CTCATGTATT TGGGACCAAC
    ACATCTAGCC TGGAACTGTT CTTGATGAAC AGAAAGATCA AAGGACCTTG TTGGCTTGAA
    GTAAAAAGTC CACAGCTCTT GAATCAGCCA GTCAGTTGGT GTAAAGTTGA GGCAATGGCT
    TTGAAACCAG ACCTGGTGAA TGTAATTAAG GATGTCAGTC CACCACCGCT TGTCGTGATG
    GCTTTCAGCA TGAAGACAAT GCAGAATGCA AAGAACCATC AAAATGAGAT TATTGCTATG
    GCAGCTTTGG TCCATCACAG TTTTGCATTG GATAAAGCAG CCCCAAAGCC TCCCTTTCAG
    TCACACTTCT GTGTTGTGTC TAAACCAAAG GACTGTATTT TTCCATATGC TTTCAAAGAA
    GTCATTGAGA AAAAGAATGT GAAGGTTGAG GTTGCTGCAA CAGAAAGAAC ACTGCTAGGT
    TTTTTCCTTG CAAAAGTTCA CAAAATTGAT CCTGATATCA TTGTGGGTCA TAATATTTAT
    GGGTTTGAAC TGGAAGTACT ACTGCAGAGA ATTAATGTGT GCAAAGCTCC TCACTGGTCC
    AAGATAGGTC GACTGAAGCG ATCCAACATG CCAAAGCTTG GGGGCCGGAG TGGATTTGGT
    GAAAGAAATG CTACCTGTGG TCGAATGATC TGTGATGTGG AAATTTCAGC AAAGGAATTG
    ATTCGTTGTA AAAGCTACCA TCTGTCTGAA CTTGTTCAGC AGATTCTAAA AACTGAAAGG
    GTTGTAATCC CAATGGAAAA TATACAAAAT ATGTACAGTG AATCTTCTCA ACTGTTATAC
    CTGTTGGAAC ACACCTGGAA AGATGCCAAG TTCATTTTGC AGATCATGTG TGAGCTAAAT
    GTTCTTCCAT TAGCATTGCA GATCACTAAC ATCGCTGGGA ACATTATGTC CAGGACGCTG
    ATGGGTGGAC GATCCGAGCG TAACGAGTTC TTGTTGCTTC ATGCATTTTA CGAAAACAAC
    TATATTGTGC CTGACAAGCA GATTTTCAGA AAGCCTCAGC AAAAACTGGG AGATGAAGAT
    GAAGAAATTG ATGGAGATAC CAATAAATAC AAGAAAGGAC GTAAGAAAGC AGCTTATGCT
    GGAGGCTTGG TTTTGGACCC CAAAGTTGGT TTTTATGATA AGTTCATTTT GCTTCTGGAC
    TTCAACAGTC TATATCCTTC CATCATTCAG GAATTTAACA TTTGTTTTAC AACAGTACAA
    AGAGTTGCTT CAGAGGCACA GAAAGTTACA GAGGATGGAG AACAAGAACA GATCCCTGAG
    TTGCCAGATC CAAGCTTAGA AATGGGCATT TTGCCCAGAG AGATCCGGAA ACTGGTAGAA
    CGGAGAAAAC AAGTCAAACA GCTAATGAAA CAGCAAGACT TAAATCCAGA CCTTATTCTT
    CAGTATGACA TTCGACAGAA GGCTTTGAAG CTCACAGCGA ACAGTATGTA TGGTTGCCTG
    GGATTTTCCT ATAGCAGATT TTACGCCAAA CCACTGGCTG CCTTGGTGAC ATACAAAGGA
    AGGGAGATTT TGATGCATAC GAAAGAGATG GTACAAAAGA TGAATCTTGA AGTTATTTAT
    GGAGATACAG ATTCAATTAT GATAAACACC AATAGCACCA ATCTGGAAGA AGTATTTAAG
    TTGGGAAACA AGGTAAAAAG TGAAGTGAAT AAGTTGTACA AACTGCTTGA AATAGACATT
    GATGGGGTTT TCAAGTCTCT GCTACTGCTG AAAAAAAAGA AGTACGCTGC TCTGGTTGTT
    GAGCCAACGT CGGATGGGAA TTATGTCACC AAACAGGAGC TCAAAGGATT AGATATAGTT
    AGAAGAGATT GGTGTGATCT TGCTAAAGAC ACTGGAAACT TTGTGATTGG CCAGATTCTT
    TCTGATCAAA GCCGGGACAC TATAGTGGAA AACATTCAGA AGAGGCTGAT AGAAATTGGA
    GAAAATGTGC TAAATGGCAG TGTCCCAGTG AGCCAGTTTG AAATTAACAA GGCATTGACA
    AAGGATCCCC AGGATTACCC TGATAAAAAA AGCCTACCTC ATGTACATGT TGCCCTCTGG
    ATAAATTCTC AAGGAGGCAG AAAGGTGAAA GCTGGAGATA CTGTGTCATA TGTCATCTGT
    CAGGATGGAT CAAACCTCAC TGCAAGTCAG AGGGCCTATG CGCCTGAGCA GCTGCAGAAA
    CAGGATAATC TAACCATTGA CACCCAGTAC TACCTGGCCC AGCAGATCCA CCCAGTCGTG
    GCTCGGATCT GTGAACCAAT AGACGGAATT GATGCTGTCC TCATTGCAAC GTGGTTGGGA
    CTTGACCCCA CCCAATTTAG AGTTCATCAT TATCATAAAG ATGAAGAGAA TGATGCTCTA
    CTTGGTGGCC CAGCACAGCT CACTGATGAA GAGAAATACA GGGACTGTGA AAGATTCAAA
    TGTCCATGCC CTACATGTGG AACTGAGAAT ATTTATGATA ATGTCTTTGA TGGTTCGGGA
    ACAGATATGG AGCCCAGCTT GTATCGTTGC AGTAACATCG ATTGTAAGGC TTCACCTCTG
    ACCTTTACAG TACAACTGAG CAACAAATTG ATCATGGACA TTAGACGTTT CATTAAAAAG
    TACTATGATG GCTGGTTGAT ATGTGAAGAG CCAACCTGTC GCAATCGAAC TCGTCACCTT
    CCCCTTCAAT TCTCCCGAAC TGGGCCTCTT TGCCCAGCCT GCATGAAAGC TACACTTCAA
    CCAGAGTATT CTGACAAGTC CCTGTACACC CAGCTGTGCT TTTACCGGTA CATTTTTGAT
    GCGGAGTGTG CACTGGAGAA ACTTACTACC GATCATGAGA AAGATAAATT GAAGAAGCAA
    TTTTTTACCC CCAAAGTTCT GCAGGACTAC AGAAAACTCA AGAACACAGC AGAGCAATTC
    TTGTCCCGAA GTGGCTACTC CGAAGTGAAT CTGAGCAAAC TCTTCGCTGG TTGTGCCGTG
    AAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_058633.2
    CDS45..4433
    Translation

    Target ORF information:

    RefSeq Version NM_016937.4
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_016937.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCACCTG TGCACGGCGA CGACTCTCTG TCAGATTCAG GGAGTTTTGT ATCTTCTCGA 
    GCCCGGCGAG AAAAAAAATC AAAGAAGGGG CGCCAAGAAG CCCTAGAAAG ACTGAAAAAG
    GCTAAAGCTG GTGAGAAGTA TAAATATGAA GTCGAGGACT TCACAGGTGT TTATGAAGAA
    GTTGATGAAG AACAGTATTC GAAGCTGGTT CAGGCACGCC AGGATGATGA CTGGATTGTG
    GATGATGATG GTATTGGCTA TGTGGAAGAT GGCCGAGAGA TTTTTGATGA TGACCTTGAA
    GATGATGCCC TTGATGCTGA TGAGAAAGGA AAAGATGGTA AAGCACGCAA TAAAGACAAG
    AGGAATGTAA AGAAGCTCGC AGTGACAAAA CCGAACAACA TTAAGTCAAT GTTCATTGCT
    TGTGCTGGAA AGAAAACTGC AGATAAAGCT GTAGACTTGT CCAAGGATGG TCTGCTAGGT
    GACATTCTAC AGGATCTTAA CACTGAGACA CCTCAAATAA CTCCACCACC TGTAATGATA
    CTGAAGAAGA AAAGATCCAT TGGAGCTTCA CCGAATCCTT TCTCTGTGCA CACCGCCACG
    GCAGTTCCTT CAGGAAAAAT TGCTTCCCCT GTCTCCAGAA AGGAGCCTCC ATTAACTCCT
    GTTCCTCTTA AACGTGCTGA ATTTGCTGGC GATGATGTAC AGGTCGAGAG TACAGAAGAA
    GAGCAGGAGT CAGGGGCAAT GGAGTTTGAA GATGGTGACT TTGATGAGCC CATGGAAGTT
    GAAGAGGTGG ACCTGGAGCC TATGGCTGCC AAGGCTTGGG ACAAAGAGAG TGAGCCAGCA
    GAGGAAGTGA AACAAGAGGC GGATTCTGGG AAAGGGACCG TGTCCTACTT AGGAAGTTTT
    CTCCCGGATG TCTCTTGTTG GGACATTGAT CAAGAAGGTG ATAGCAGTTT CTCAGTGCAA
    GAAGTTCAAG TGGATTCCAG TCACCTCCCA TTGGTAAAAG GGGCAGATGA GGAACAAGTA
    TTCCACTTTT ATTGGTTGGA TGCTTATGAG GATCAGTACA ACCAACCAGG TGTGGTATTT
    CTGTTTGGGA AAGTTTGGAT TGAATCAGCC GAGACCCATG TGAGCTGTTG TGTCATGGTG
    AAAAATATCG AGCGAACGCT TTACTTCCTT CCCCGTGAAA TGAAAATTGA TCTAAATACG
    GGGAAAGAAA CAGGAACTCC AATTTCAATG AAGGATGTTT ATGAGGAATT TGATGAGAAA
    ATAGCAACAA AATATAAAAT TATGAAGTTC AAGTCTAAGC CAGTGGAAAA GAACTATGCT
    TTTGAGATAC CTGATGTTCC AGAAAAATCT GAGTACTTGG AAGTTAAATA CTCGGCTGAA
    ATGCCACAGC TTCCTCAAGA TTTGAAAGGA GAAACTTTTT CTCATGTATT TGGGACCAAC
    ACATCTAGCC TGGAACTGTT CTTGATGAAC AGAAAGATCA AAGGACCTTG TTGGCTTGAA
    GTAAAAAGTC CACAGCTCTT GAATCAGCCA GTCAGTTGGT GTAAAGTTGA GGCAATGGCT
    TTGAAACCAG ACCTGGTGAA TGTAATTAAG GATGTCAGTC CACCACCGCT TGTCGTGATG
    GCTTTCAGCA TGAAGACAAT GCAGAATGCA AAGAACCATC AAAATGAGAT TATTGCTATG
    GCAGCTTTGG TCCATCACAG TTTTGCATTG GATAAAGCAG CCCCAAAGCC TCCCTTTCAG
    TCACACTTCT GTGTTGTGTC TAAACCAAAG GACTGTATTT TTCCATATGC TTTCAAAGAA
    GTCATTGAGA AAAAGAATGT GAAGGTTGAG GTTGCTGCAA CAGAAAGAAC ACTGCTAGGT
    TTTTTCCTTG CAAAAGTTCA CAAAATTGAT CCTGATATCA TTGTGGGTCA TAATATTTAT
    GGGTTTGAAC TGGAAGTACT ACTGCAGAGA ATTAATGTGT GCAAAGCTCC TCACTGGTCC
    AAGATAGGTC GACTGAAGCG ATCCAACATG CCAAAGCTTG GGGGCCGGAG TGGATTTGGT
    GAAAGAAATG CTACCTGTGG TCGAATGATC TGTGATGTGG AAATTTCAGC AAAGGAATTG
    ATTCGTTGTA AAAGCTACCA TCTGTCTGAA CTTGTTCAGC AGATTCTAAA AACTGAAAGG
    GTTGTAATCC CAATGGAAAA TATACAAAAT ATGTACAGTG AATCTTCTCA ACTGTTATAC
    CTGTTGGAAC ACACCTGGAA AGATGCCAAG TTCATTTTGC AGATCATGTG TGAGCTAAAT
    GTTCTTCCAT TAGCATTGCA GATCACTAAC ATCGCTGGGA ACATTATGTC CAGGACGCTG
    ATGGGTGGAC GATCCGAGCG TAACGAGTTC TTGTTGCTTC ATGCATTTTA CGAAAACAAC
    TATATTGTGC CTGACAAGCA GATTTTCAGA AAGCCTCAGC AAAAACTGGG AGATGAAGAT
    GAAGAAATTG ATGGAGATAC CAATAAATAC AAGAAAGGAC GTAAGAAAGC AGCTTATGCT
    GGAGGCTTGG TTTTGGACCC CAAAGTTGGT TTTTATGATA AGTTCATTTT GCTTCTGGAC
    TTCAACAGTC TATATCCTTC CATCATTCAG GAATTTAACA TTTGTTTTAC AACAGTACAA
    AGAGTTGCTT CAGAGGCACA GAAAGTTACA GAGGATGGAG AACAAGAACA GATCCCTGAG
    TTGCCAGATC CAAGCTTAGA AATGGGCATT TTGCCCAGAG AGATCCGGAA ACTGGTAGAA
    CGGAGAAAAC AAGTCAAACA GCTAATGAAA CAGCAAGACT TAAATCCAGA CCTTATTCTT
    CAGTATGACA TTCGACAGAA GGCTTTGAAG CTCACAGCGA ACAGTATGTA TGGTTGCCTG
    GGATTTTCCT ATAGCAGATT TTACGCCAAA CCACTGGCTG CCTTGGTGAC ATACAAAGGA
    AGGGAGATTT TGATGCATAC GAAAGAGATG GTACAAAAGA TGAATCTTGA AGTTATTTAT
    GGAGATACAG ATTCAATTAT GATAAACACC AATAGCACCA ATCTGGAAGA AGTATTTAAG
    TTGGGAAACA AGGTAAAAAG TGAAGTGAAT AAGTTGTACA AACTGCTTGA AATAGACATT
    GATGGGGTTT TCAAGTCTCT GCTACTGCTG AAAAAAAAGA AGTACGCTGC TCTGGTTGTT
    GAGCCAACGT CGGATGGGAA TTATGTCACC AAACAGGAGC TCAAAGGATT AGATATAGTT
    AGAAGAGATT GGTGTGATCT TGCTAAAGAC ACTGGAAACT TTGTGATTGG CCAGATTCTT
    TCTGATCAAA GCCGGGACAC TATAGTGGAA AACATTCAGA AGAGGCTGAT AGAAATTGGA
    GAAAATGTGC TAAATGGCAG TGTCCCAGTG AGCCAGTTTG AAATTAACAA GGCATTGACA
    AAGGATCCCC AGGATTACCC TGATAAAAAA AGCCTACCTC ATGTACATGT TGCCCTCTGG
    ATAAATTCTC AAGGAGGCAG AAAGGTGAAA GCTGGAGATA CTGTGTCATA TGTCATCTGT
    CAGGATGGAT CAAACCTCAC TGCAAGTCAG AGGGCCTATG CGCCTGAGCA GCTGCAGAAA
    CAGGATAATC TAACCATTGA CACCCAGTAC TACCTGGCCC AGCAGATCCA CCCAGTCGTG
    GCTCGGATCT GTGAACCAAT AGACGGAATT GATGCTGTCC TCATTGCAAC GTGGTTGGGA
    CTTGACCCCA CCCAATTTAG AGTTCATCAT TATCATAAAG ATGAAGAGAA TGATGCTCTA
    CTTGGTGGCC CAGCACAGCT CACTGATGAA GAGAAATACA GGGACTGTGA AAGATTCAAA
    TGTCCATGCC CTACATGTGG AACTGAGAAT ATTTATGATA ATGTCTTTGA TGGTTCGGGA
    ACAGATATGG AGCCCAGCTT GTATCGTTGC AGTAACATCG ATTGTAAGGC TTCACCTCTG
    ACCTTTACAG TACAACTGAG CAACAAATTG ATCATGGACA TTAGACGTTT CATTAAAAAG
    TACTATGATG GCTGGTTGAT ATGTGAAGAG CCAACCTGTC GCAATCGAAC TCGTCACCTT
    CCCCTTCAAT TCTCCCGAAC TGGGCCTCTT TGCCCAGCCT GCATGAAAGC TACACTTCAA
    CCAGAGTATT CTGACAAGTC CCTGTACACC CAGCTGTGCT TTTACCGGTA CATTTTTGAT
    GCGGAGTGTG CACTGGAGAA ACTTACTACC GATCATGAGA AAGATAAATT GAAGAAGCAA
    TTTTTTACCC CCAAAGTTCT GCAGGACTAC AGAAAACTCA AGAACACAGC AGAGCAATTC
    TTGTCCCGAA GTGGCTACTC CGAAGTGAAT CTGAGCAAAC TCTTCGCTGG TTGTGCCGTG
    AAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53935
    Clone ID Related Accession (Same CDS sequence) XM_006724499.2
    Accession Version XM_006724499.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3981bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006724499.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTCATTG CTTGTGCTGG AAAGAAAACT GCAGATAAAG CTGTAGACTT GTCCAAGGAT 
    GGTCTGCTAG GTGACATTCT ACAGGATCTT AACACTGAGA CACCTCAAAT AACTCCACCA
    CCTGTAATGA TACTGAAGAA GAAAAGATCC ATTGGAGCTT CACCGAATCC TTTCTCTGTG
    CACACCGCCA CGGCAGTTCC TTCAGGAAAA ATTGCTTCCC CTGTCTCCAG AAAGGAGCCT
    CCATTAACTC CTGTTCCTCT TAAACGTGCT GAATTTGCTG GCGATGATGT ACAGGTCGAG
    AGTACAGAAG AAGAGCAGGA GTCAGGGGCA ATGGAGTTTG AAGATGGTGA CTTTGATGAG
    CCCATGGAAG TTGAAGAGGT GGACCTGGAG CCTATGGCTG CCAAGGCTTG GGACAAAGAG
    AGTGAGCCAG CAGAGGAAGT GAAACAAGAG GCGGATTCTG GGAAAGGGAC CGTGTCCTAC
    TTAGGAAGTT TTCTCCCGGA TGTCTCTTGT TGGGACATTG ATCAAGAAGG TGATAGCAGT
    TTCTCAGTGC AAGAAGTTCA AGTGGATTCC AGTCACCTCC CATTGGTAAA AGGGGCAGAT
    GAGGAACAAG TATTCCACTT TTATTGGTTG GATGCTTATG AGGATCAGTA CAACCAACCA
    GGTGTGGTAT TTCTGTTTGG GAAAGTTTGG ATTGAATCAG CCGAGACCCA TGTGAGCTGT
    TGTGTCATGG TGAAAAATAT CGAGCGAACG CTTTACTTCC TTCCCCGTGA AATGAAAATT
    GATCTAAATA CGGGGAAAGA AACAGGAACT CCAATTTCAA TGAAGGATGT TTATGAGGAA
    TTTGATGAGA AAATAGCAAC AAAATATAAA ATTATGAAGT TCAAGTCTAA GCCAGTGGAA
    AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACTT GGAAGTTAAA
    TACTCGGCTG AAATGCCACA GCTTCCTCAA GATTTGAAAG GAGAAACTTT TTCTCATGTA
    TTTGGGACCA ACACATCTAG CCTGGAACTG TTCTTGATGA ACAGAAAGAT CAAAGGACCT
    TGTTGGCTTG AAGTAAAAAG TCCACAGCTC TTGAATCAGC CAGTCAGTTG GTGTAAAGTT
    GAGGCAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCACCACCG
    CTTGTCGTGA TGGCTTTCAG CATGAAGACA ATGCAGAATG CAAAGAACCA TCAAAATGAG
    ATTATTGCTA TGGCAGCTTT GGTCCATCAC AGTTTTGCAT TGGATAAAGC AGCCCCAAAG
    CCTCCCTTTC AGTCACACTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTTCCATAT
    GCTTTCAAAG AAGTCATTGA GAAAAAGAAT GTGAAGGTTG AGGTTGCTGC AACAGAAAGA
    ACACTGCTAG GTTTTTTCCT TGCAAAAGTT CACAAAATTG ATCCTGATAT CATTGTGGGT
    CATAATATTT ATGGGTTTGA ACTGGAAGTA CTACTGCAGA GAATTAATGT GTGCAAAGCT
    CCTCACTGGT CCAAGATAGG TCGACTGAAG CGATCCAACA TGCCAAAGCT TGGGGGCCGG
    AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGTCGAATGA TCTGTGATGT GGAAATTTCA
    GCAAAGGAAT TGATTCGTTG TAAAAGCTAC CATCTGTCTG AACTTGTTCA GCAGATTCTA
    AAAACTGAAA GGGTTGTAAT CCCAATGGAA AATATACAAA ATATGTACAG TGAATCTTCT
    CAACTGTTAT ACCTGTTGGA ACACACCTGG AAAGATGCCA AGTTCATTTT GCAGATCATG
    TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG
    TCCAGGACGC TGATGGGTGG ACGATCCGAG CGTAACGAGT TCTTGTTGCT TCATGCATTT
    TACGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAGCCTCA GCAAAAACTG
    GGAGATGAAG ATGAAGAAAT TGATGGAGAT ACCAATAAAT ACAAGAAAGG ACGTAAGAAA
    GCAGCTTATG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA TAAGTTCATT
    TTGCTTCTGG ACTTCAACAG TCTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT
    ACAACAGTAC AAAGAGTTGC TTCAGAGGCA CAGAAAGTTA CAGAGGATGG AGAACAAGAA
    CAGATCCCTG AGTTGCCAGA TCCAAGCTTA GAAATGGGCA TTTTGCCCAG AGAGATCCGG
    AAACTGGTAG AACGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA CTTAAATCCA
    GACCTTATTC TTCAGTATGA CATTCGACAG AAGGCTTTGA AGCTCACAGC GAACAGTATG
    TATGGTTGCC TGGGATTTTC CTATAGCAGA TTTTACGCCA AACCACTGGC TGCCTTGGTG
    ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGAGA TGGTACAAAA GATGAATCTT
    GAAGTTATTT ATGGAGATAC AGATTCAATT ATGATAAACA CCAATAGCAC CAATCTGGAA
    GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAGTTGTA CAAACTGCTT
    GAAATAGACA TTGATGGGGT TTTCAAGTCT CTGCTACTGC TGAAAAAAAA GAAGTACGCT
    GCTCTGGTTG TTGAGCCAAC GTCGGATGGG AATTATGTCA CCAAACAGGA GCTCAAAGGA
    TTAGATATAG TTAGAAGAGA TTGGTGTGAT CTTGCTAAAG ACACTGGAAA CTTTGTGATT
    GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACTATAGTGG AAAACATTCA GAAGAGGCTG
    ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC
    AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAAAA AAAGCCTACC TCATGTACAT
    GTTGCCCTCT GGATAAATTC TCAAGGAGGC AGAAAGGTGA AAGCTGGAGA TACTGTGTCA
    TATGTCATCT GTCAGGATGG ATCAAACCTC ACTGCAAGTC AGAGGGCCTA TGCGCCTGAG
    CAGCTGCAGA AACAGGATAA TCTAACCATT GACACCCAGT ACTACCTGGC CCAGCAGATC
    CACCCAGTCG TGGCTCGGAT CTGTGAACCA ATAGACGGAA TTGATGCTGT CCTCATTGCA
    ACGTGGTTGG GACTTGACCC CACCCAATTT AGAGTTCATC ATTATCATAA AGATGAAGAG
    AATGATGCTC TACTTGGTGG CCCAGCACAG CTCACTGATG AAGAGAAATA CAGGGACTGT
    GAAAGATTCA AATGTCCATG CCCTACATGT GGAACTGAGA ATATTTATGA TAATGTCTTT
    GATGGTTCGG GAACAGATAT GGAGCCCAGC TTGTATCGTT GCAGTAACAT CGATTGTAAG
    GCTTCACCTC TGACCTTTAC AGTACAACTG AGCAACAAAT TGATCATGGA CATTAGACGT
    TTCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACCTG TCGCAATCGA
    ACTCGTCACC TTCCCCTTCA ATTCTCCCGA ACTGGGCCTC TTTGCCCAGC CTGCATGAAA
    GCTACACTTC AACCAGAGTA TTCTGACAAG TCCCTGTACA CCCAGCTGTG CTTTTACCGG
    TACATTTTTG ATGCGGAGTG TGCACTGGAG AAACTTACTA CCGATCATGA GAAAGATAAA
    TTGAAGAAGC AATTTTTTAC CCCCAAAGTT CTGCAGGACT ACAGAAAACT CAAGAACACA
    GCAGAGCAAT TCTTGTCCCG AAGTGGCTAC TCCGAAGTGA ATCTGAGCAA ACTCTTCGCT
    GGTTGTGCCG TGAAATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006724562.1
    CDS366..4346
    Translation

    Target ORF information:

    RefSeq Version XM_006724499.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006724499.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTCATTG CTTGTGCTGG AAAGAAAACT GCAGATAAAG CTGTAGACTT GTCCAAGGAT 
    GGTCTGCTAG GTGACATTCT ACAGGATCTT AACACTGAGA CACCTCAAAT AACTCCACCA
    CCTGTAATGA TACTGAAGAA GAAAAGATCC ATTGGAGCTT CACCGAATCC TTTCTCTGTG
    CACACCGCCA CGGCAGTTCC TTCAGGAAAA ATTGCTTCCC CTGTCTCCAG AAAGGAGCCT
    CCATTAACTC CTGTTCCTCT TAAACGTGCT GAATTTGCTG GCGATGATGT ACAGGTCGAG
    AGTACAGAAG AAGAGCAGGA GTCAGGGGCA ATGGAGTTTG AAGATGGTGA CTTTGATGAG
    CCCATGGAAG TTGAAGAGGT GGACCTGGAG CCTATGGCTG CCAAGGCTTG GGACAAAGAG
    AGTGAGCCAG CAGAGGAAGT GAAACAAGAG GCGGATTCTG GGAAAGGGAC CGTGTCCTAC
    TTAGGAAGTT TTCTCCCGGA TGTCTCTTGT TGGGACATTG ATCAAGAAGG TGATAGCAGT
    TTCTCAGTGC AAGAAGTTCA AGTGGATTCC AGTCACCTCC CATTGGTAAA AGGGGCAGAT
    GAGGAACAAG TATTCCACTT TTATTGGTTG GATGCTTATG AGGATCAGTA CAACCAACCA
    GGTGTGGTAT TTCTGTTTGG GAAAGTTTGG ATTGAATCAG CCGAGACCCA TGTGAGCTGT
    TGTGTCATGG TGAAAAATAT CGAGCGAACG CTTTACTTCC TTCCCCGTGA AATGAAAATT
    GATCTAAATA CGGGGAAAGA AACAGGAACT CCAATTTCAA TGAAGGATGT TTATGAGGAA
    TTTGATGAGA AAATAGCAAC AAAATATAAA ATTATGAAGT TCAAGTCTAA GCCAGTGGAA
    AAGAACTATG CTTTTGAGAT ACCTGATGTT CCAGAAAAAT CTGAGTACTT GGAAGTTAAA
    TACTCGGCTG AAATGCCACA GCTTCCTCAA GATTTGAAAG GAGAAACTTT TTCTCATGTA
    TTTGGGACCA ACACATCTAG CCTGGAACTG TTCTTGATGA ACAGAAAGAT CAAAGGACCT
    TGTTGGCTTG AAGTAAAAAG TCCACAGCTC TTGAATCAGC CAGTCAGTTG GTGTAAAGTT
    GAGGCAATGG CTTTGAAACC AGACCTGGTG AATGTAATTA AGGATGTCAG TCCACCACCG
    CTTGTCGTGA TGGCTTTCAG CATGAAGACA ATGCAGAATG CAAAGAACCA TCAAAATGAG
    ATTATTGCTA TGGCAGCTTT GGTCCATCAC AGTTTTGCAT TGGATAAAGC AGCCCCAAAG
    CCTCCCTTTC AGTCACACTT CTGTGTTGTG TCTAAACCAA AGGACTGTAT TTTTCCATAT
    GCTTTCAAAG AAGTCATTGA GAAAAAGAAT GTGAAGGTTG AGGTTGCTGC AACAGAAAGA
    ACACTGCTAG GTTTTTTCCT TGCAAAAGTT CACAAAATTG ATCCTGATAT CATTGTGGGT
    CATAATATTT ATGGGTTTGA ACTGGAAGTA CTACTGCAGA GAATTAATGT GTGCAAAGCT
    CCTCACTGGT CCAAGATAGG TCGACTGAAG CGATCCAACA TGCCAAAGCT TGGGGGCCGG
    AGTGGATTTG GTGAAAGAAA TGCTACCTGT GGTCGAATGA TCTGTGATGT GGAAATTTCA
    GCAAAGGAAT TGATTCGTTG TAAAAGCTAC CATCTGTCTG AACTTGTTCA GCAGATTCTA
    AAAACTGAAA GGGTTGTAAT CCCAATGGAA AATATACAAA ATATGTACAG TGAATCTTCT
    CAACTGTTAT ACCTGTTGGA ACACACCTGG AAAGATGCCA AGTTCATTTT GCAGATCATG
    TGTGAGCTAA ATGTTCTTCC ATTAGCATTG CAGATCACTA ACATCGCTGG GAACATTATG
    TCCAGGACGC TGATGGGTGG ACGATCCGAG CGTAACGAGT TCTTGTTGCT TCATGCATTT
    TACGAAAACA ACTATATTGT GCCTGACAAG CAGATTTTCA GAAAGCCTCA GCAAAAACTG
    GGAGATGAAG ATGAAGAAAT TGATGGAGAT ACCAATAAAT ACAAGAAAGG ACGTAAGAAA
    GCAGCTTATG CTGGAGGCTT GGTTTTGGAC CCCAAAGTTG GTTTTTATGA TAAGTTCATT
    TTGCTTCTGG ACTTCAACAG TCTATATCCT TCCATCATTC AGGAATTTAA CATTTGTTTT
    ACAACAGTAC AAAGAGTTGC TTCAGAGGCA CAGAAAGTTA CAGAGGATGG AGAACAAGAA
    CAGATCCCTG AGTTGCCAGA TCCAAGCTTA GAAATGGGCA TTTTGCCCAG AGAGATCCGG
    AAACTGGTAG AACGGAGAAA ACAAGTCAAA CAGCTAATGA AACAGCAAGA CTTAAATCCA
    GACCTTATTC TTCAGTATGA CATTCGACAG AAGGCTTTGA AGCTCACAGC GAACAGTATG
    TATGGTTGCC TGGGATTTTC CTATAGCAGA TTTTACGCCA AACCACTGGC TGCCTTGGTG
    ACATACAAAG GAAGGGAGAT TTTGATGCAT ACGAAAGAGA TGGTACAAAA GATGAATCTT
    GAAGTTATTT ATGGAGATAC AGATTCAATT ATGATAAACA CCAATAGCAC CAATCTGGAA
    GAAGTATTTA AGTTGGGAAA CAAGGTAAAA AGTGAAGTGA ATAAGTTGTA CAAACTGCTT
    GAAATAGACA TTGATGGGGT TTTCAAGTCT CTGCTACTGC TGAAAAAAAA GAAGTACGCT
    GCTCTGGTTG TTGAGCCAAC GTCGGATGGG AATTATGTCA CCAAACAGGA GCTCAAAGGA
    TTAGATATAG TTAGAAGAGA TTGGTGTGAT CTTGCTAAAG ACACTGGAAA CTTTGTGATT
    GGCCAGATTC TTTCTGATCA AAGCCGGGAC ACTATAGTGG AAAACATTCA GAAGAGGCTG
    ATAGAAATTG GAGAAAATGT GCTAAATGGC AGTGTCCCAG TGAGCCAGTT TGAAATTAAC
    AAGGCATTGA CAAAGGATCC CCAGGATTAC CCTGATAAAA AAAGCCTACC TCATGTACAT
    GTTGCCCTCT GGATAAATTC TCAAGGAGGC AGAAAGGTGA AAGCTGGAGA TACTGTGTCA
    TATGTCATCT GTCAGGATGG ATCAAACCTC ACTGCAAGTC AGAGGGCCTA TGCGCCTGAG
    CAGCTGCAGA AACAGGATAA TCTAACCATT GACACCCAGT ACTACCTGGC CCAGCAGATC
    CACCCAGTCG TGGCTCGGAT CTGTGAACCA ATAGACGGAA TTGATGCTGT CCTCATTGCA
    ACGTGGTTGG GACTTGACCC CACCCAATTT AGAGTTCATC ATTATCATAA AGATGAAGAG
    AATGATGCTC TACTTGGTGG CCCAGCACAG CTCACTGATG AAGAGAAATA CAGGGACTGT
    GAAAGATTCA AATGTCCATG CCCTACATGT GGAACTGAGA ATATTTATGA TAATGTCTTT
    GATGGTTCGG GAACAGATAT GGAGCCCAGC TTGTATCGTT GCAGTAACAT CGATTGTAAG
    GCTTCACCTC TGACCTTTAC AGTACAACTG AGCAACAAAT TGATCATGGA CATTAGACGT
    TTCATTAAAA AGTACTATGA TGGCTGGTTG ATATGTGAAG AGCCAACCTG TCGCAATCGA
    ACTCGTCACC TTCCCCTTCA ATTCTCCCGA ACTGGGCCTC TTTGCCCAGC CTGCATGAAA
    GCTACACTTC AACCAGAGTA TTCTGACAAG TCCCTGTACA CCCAGCTGTG CTTTTACCGG
    TACATTTTTG ATGCGGAGTG TGCACTGGAG AAACTTACTA CCGATCATGA GAAAGATAAA
    TTGAAGAAGC AATTTTTTAC CCCCAAAGTT CTGCAGGACT ACAGAAAACT CAAGAACACA
    GCAGAGCAAT TCTTGTCCCG AAGTGGCTAC TCCGAAGTGA ATCTGAGCAA ACTCTTCGCT
    GGTTGTGCCG TGAAATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu106266
    Clone ID Related Accession (Same CDS sequence) XM_024452392.1
    Accession Version XM_024452392.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GCAAATGTCC AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308160.1
    CDS85..3660
    Translation

    Target ORF information:

    RefSeq Version XM_024452392.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452392.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GCAAATGTCC AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99340
    Clone ID Related Accession (Same CDS sequence) XM_017029594.2 , XM_017029594.1
    Accession Version XM_017029594.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029594.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATGGC TGGTTGATAT GTGAAGAGCC AACCTGTCGC
    AATCGAACTC GTCACCTTCC CCTTCAATTC TCCCGAACTG GGCCTCTTTG CCCAGCCTGC
    ATGAAAGCTA CACTTCAACC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTACTACCGA TCATGAGAAA
    GGATGGCCGC ATGAAATGGT CCCATTTGGC TGCCTCATCC CGTGGTGCCT TGGCCTGAGT
    AGGGGTAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885083.1
    CDS86..4417
    Translation

    Target ORF information:

    RefSeq Version XM_017029594.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029594.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATGGC TGGTTGATAT GTGAAGAGCC AACCTGTCGC
    AATCGAACTC GTCACCTTCC CCTTCAATTC TCCCGAACTG GGCCTCTTTG CCCAGCCTGC
    ATGAAAGCTA CACTTCAACC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTACTACCGA TCATGAGAAA
    GGATGGCCGC ATGAAATGGT CCCATTTGGC TGCCTCATCC CGTGGTGCCT TGGCCTGAGT
    AGGGGTAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99340
    Clone ID Related Accession (Same CDS sequence) XM_017029594.2 , XM_017029594.1
    Accession Version XM_017029594.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATGGC TGGTTGATAT GTGAAGAGCC AACCTGTCGC
    AATCGAACTC GTCACCTTCC CCTTCAATTC TCCCGAACTG GGCCTCTTTG CCCAGCCTGC
    ATGAAAGCTA CACTTCAACC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTACTACCGA TCATGAGAAA
    GGATGGCCGC ATGAAATGGT CCCATTTGGC TGCCTCATCC CGTGGTGCCT TGGCCTGAGT
    AGGGGTAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885083.1
    CDS86..4417
    Translation

    Target ORF information:

    RefSeq Version XM_017029594.1
    Organism Homo sapiens(human)
    Definition Homo sapiens polymerase (DNA) alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029594.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATGGC TGGTTGATAT GTGAAGAGCC AACCTGTCGC
    AATCGAACTC GTCACCTTCC CCTTCAATTC TCCCGAACTG GGCCTCTTTG CCCAGCCTGC
    ATGAAAGCTA CACTTCAACC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTACTACCGA TCATGAGAAA
    GGATGGCCGC ATGAAATGGT CCCATTTGGC TGCCTCATCC CGTGGTGCCT TGGCCTGAGT
    AGGGGTAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99341
    Clone ID Related Accession (Same CDS sequence) XM_017029595.1 , XM_017029595.2
    Accession Version XM_017029595.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATGGC TGGTTGATAT GTGAAGAGCC AACCTGTCGC
    AATCGAACTC GTCACCTTCC CCTTCAATTC TCCCGAACTG GGCCTCTTTG CCCAGCCTGC
    ATGAAAGCTA CACTTCAACC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTACTACCGA TCATGAGAAA
    GGCCTGGTGG TAACATCTGT AGGCCCTCTT GTTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885084.1
    CDS86..4384
    Translation

    Target ORF information:

    RefSeq Version XM_017029595.1
    Organism Homo sapiens(human)
    Definition Homo sapiens polymerase (DNA) alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029595.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATGGC TGGTTGATAT GTGAAGAGCC AACCTGTCGC
    AATCGAACTC GTCACCTTCC CCTTCAATTC TCCCGAACTG GGCCTCTTTG CCCAGCCTGC
    ATGAAAGCTA CACTTCAACC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTACTACCGA TCATGAGAAA
    GGCCTGGTGG TAACATCTGT AGGCCCTCTT GTTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99341
    Clone ID Related Accession (Same CDS sequence) XM_017029595.1 , XM_017029595.2
    Accession Version XM_017029595.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATGGC TGGTTGATAT GTGAAGAGCC AACCTGTCGC
    AATCGAACTC GTCACCTTCC CCTTCAATTC TCCCGAACTG GGCCTCTTTG CCCAGCCTGC
    ATGAAAGCTA CACTTCAACC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTACTACCGA TCATGAGAAA
    GGCCTGGTGG TAACATCTGT AGGCCCTCTT GTTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885084.1
    CDS86..4384
    Translation

    Target ORF information:

    RefSeq Version XM_017029595.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029595.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATGGC TGGTTGATAT GTGAAGAGCC AACCTGTCGC
    AATCGAACTC GTCACCTTCC CCTTCAATTC TCCCGAACTG GGCCTCTTTG CCCAGCCTGC
    ATGAAAGCTA CACTTCAACC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTACTACCGA TCATGAGAAA
    GGCCTGGTGG TAACATCTGT AGGCCCTCTT GTTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99342
    Clone ID Related Accession (Same CDS sequence) XM_017029596.1
    Accession Version XM_017029596.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATTTC TGTGAGTGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885085.1
    CDS86..4147
    Translation

    Target ORF information:

    RefSeq Version XM_017029596.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029596.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATTTC TGTGAGTGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53934
    Clone ID Related Accession (Same CDS sequence) NM_001330360.1 , NM_001330360.2
    Accession Version NM_001330360.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-24
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004655.1, AC079375.24, KF459037.1, AC135280.2 and AC002504.1. On Aug 25, 2016 this sequence version replaced XM_005274552.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.108337.1, SRR1803613.231149.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATGGC TGGTTGATAT GTGAAGAGCC AACCTGTCGC
    AATCGAACTC GTCACCTTCC CCTTCAATTC TCCCGAACTG GGCCTCTTTG CCCAGCCTGC
    ATGAAAGCTA CACTTCAACC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTACTACCGA TCATGAGAAA
    GATAAATTGA AGAAGCAATT TTTTACCCCC AAAGTTCTGC AGGACTACAG AAAACTCAAG
    AACACAGCAG AGCAATTCTT GTCCCGAAGT GGCTACTCCG AAGTGAATCT GAGCAAACTC
    TTCGCTGGTT GTGCCGTGAA ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317289.1
    CDS130..4536
    Translation

    Target ORF information:

    RefSeq Version NM_001330360.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330360.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATGGC TGGTTGATAT GTGAAGAGCC AACCTGTCGC
    AATCGAACTC GTCACCTTCC CCTTCAATTC TCCCGAACTG GGCCTCTTTG CCCAGCCTGC
    ATGAAAGCTA CACTTCAACC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTACTACCGA TCATGAGAAA
    GATAAATTGA AGAAGCAATT TTTTACCCCC AAAGTTCTGC AGGACTACAG AAAACTCAAG
    AACACAGCAG AGCAATTCTT GTCCCGAAGT GGCTACTCCG AAGTGAATCT GAGCAAACTC
    TTCGCTGGTT GTGCCGTGAA ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53934
    Clone ID Related Accession (Same CDS sequence) NM_001330360.1 , NM_001330360.2
    Accession Version NM_001330360.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004655.1, AC079375.24, KF459037.1, AC135280.2 and AC002504.1. On Jun 2, 2019 this sequence version replaced NM_001330360.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.189376.1, SRR1803613.231149.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATGGC TGGTTGATAT GTGAAGAGCC AACCTGTCGC
    AATCGAACTC GTCACCTTCC CCTTCAATTC TCCCGAACTG GGCCTCTTTG CCCAGCCTGC
    ATGAAAGCTA CACTTCAACC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTACTACCGA TCATGAGAAA
    GATAAATTGA AGAAGCAATT TTTTACCCCC AAAGTTCTGC AGGACTACAG AAAACTCAAG
    AACACAGCAG AGCAATTCTT GTCCCGAAGT GGCTACTCCG AAGTGAATCT GAGCAAACTC
    TTCGCTGGTT GTGCCGTGAA ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317289.1
    CDS45..4451
    Translation

    Target ORF information:

    RefSeq Version NM_001330360.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330360.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATGGC TGGTTGATAT GTGAAGAGCC AACCTGTCGC
    AATCGAACTC GTCACCTTCC CCTTCAATTC TCCCGAACTG GGCCTCTTTG CCCAGCCTGC
    ATGAAAGCTA CACTTCAACC AGAGTATTCT GACAAGTCCC TGTACACCCA GCTGTGCTTT
    TACCGGTACA TTTTTGATGC GGAGTGTGCA CTGGAGAAAC TTACTACCGA TCATGAGAAA
    GATAAATTGA AGAAGCAATT TTTTACCCCC AAAGTTCTGC AGGACTACAG AAAACTCAAG
    AACACAGCAG AGCAATTCTT GTCCCGAAGT GGCTACTCCG AAGTGAATCT GAGCAAACTC
    TTCGCTGGTT GTGCCGTGAA ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59805
    Clone ID Related Accession (Same CDS sequence) XM_011545540.2 , XM_011545540.3
    Accession Version XM_011545540.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543842.1
    CDS85..4137
    Translation

    Target ORF information:

    RefSeq Version XM_011545540.2
    Organism Homo sapiens(human)
    Definition Homo sapiens polymerase (DNA) alpha 1, catalytic subunit (POLA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545540.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59805
    Clone ID Related Accession (Same CDS sequence) XM_011545540.2 , XM_011545540.3
    Accession Version XM_011545540.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA polymerase alpha catalytic subunit isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545540.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543842.1
    CDS86..4138
    Translation

    Target ORF information:

    RefSeq Version XM_011545540.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA polymerase alpha 1, catalytic subunit (POLA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545540.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCACCTG TGCACGGCGA CGACTGTGAG ATAGGGGCGA GTGCTCTGTC AGATTCAGGG 
    AGTTTTGTAT CTTCTCGAGC CCGGCGAGAA AAAAAATCAA AGAAGGGGCG CCAAGAAGCC
    CTAGAAAGAC TGAAAAAGGC TAAAGCTGGT GAGAAGTATA AATATGAAGT CGAGGACTTC
    ACAGGTGTTT ATGAAGAAGT TGATGAAGAA CAGTATTCGA AGCTGGTTCA GGCACGCCAG
    GATGATGACT GGATTGTGGA TGATGATGGT ATTGGCTATG TGGAAGATGG CCGAGAGATT
    TTTGATGATG ACCTTGAAGA TGATGCCCTT GATGCTGATG AGAAAGGAAA AGATGGTAAA
    GCACGCAATA AAGACAAGAG GAATGTAAAG AAGCTCGCAG TGACAAAACC GAACAACATT
    AAGTCAATGT TCATTGCTTG TGCTGGAAAG AAAACTGCAG ATAAAGCTGT AGACTTGTCC
    AAGGATGGTC TGCTAGGTGA CATTCTACAG GATCTTAACA CTGAGACACC TCAAATAACT
    CCACCACCTG TAATGATACT GAAGAAGAAA AGATCCATTG GAGCTTCACC GAATCCTTTC
    TCTGTGCACA CCGCCACGGC AGTTCCTTCA GGAAAAATTG CTTCCCCTGT CTCCAGAAAG
    GAGCCTCCAT TAACTCCTGT TCCTCTTAAA CGTGCTGAAT TTGCTGGCGA TGATGTACAG
    GTCGAGAGTA CAGAAGAAGA GCAGGAGTCA GGGGCAATGG AGTTTGAAGA TGGTGACTTT
    GATGAGCCCA TGGAAGTTGA AGAGGTGGAC CTGGAGCCTA TGGCTGCCAA GGCTTGGGAC
    AAAGAGAGTG AGCCAGCAGA GGAAGTGAAA CAAGAGGCGG ATTCTGGGAA AGGGACCGTG
    TCCTACTTAG GAAGTTTTCT CCCGGATGTC TCTTGTTGGG ACATTGATCA AGAAGGTGAT
    AGCAGTTTCT CAGTGCAAGA AGTTCAAGTG GATTCCAGTC ACCTCCCATT GGTAAAAGGG
    GCAGATGAGG AACAAGTATT CCACTTTTAT TGGTTGGATG CTTATGAGGA TCAGTACAAC
    CAACCAGGTG TGGTATTTCT GTTTGGGAAA GTTTGGATTG AATCAGCCGA GACCCATGTG
    AGCTGTTGTG TCATGGTGAA AAATATCGAG CGAACGCTTT ACTTCCTTCC CCGTGAAATG
    AAAATTGATC TAAATACGGG GAAAGAAACA GGAACTCCAA TTTCAATGAA GGATGTTTAT
    GAGGAATTTG ATGAGAAAAT AGCAACAAAA TATAAAATTA TGAAGTTCAA GTCTAAGCCA
    GTGGAAAAGA ACTATGCTTT TGAGATACCT GATGTTCCAG AAAAATCTGA GTACTTGGAA
    GTTAAATACT CGGCTGAAAT GCCACAGCTT CCTCAAGATT TGAAAGGAGA AACTTTTTCT
    CATGTATTTG GGACCAACAC ATCTAGCCTG GAACTGTTCT TGATGAACAG AAAGATCAAA
    GGACCTTGTT GGCTTGAAGT AAAAAGTCCA CAGCTCTTGA ATCAGCCAGT CAGTTGGTGT
    AAAGTTGAGG CAATGGCTTT GAAACCAGAC CTGGTGAATG TAATTAAGGA TGTCAGTCCA
    CCACCGCTTG TCGTGATGGC TTTCAGCATG AAGACAATGC AGAATGCAAA GAACCATCAA
    AATGAGATTA TTGCTATGGC AGCTTTGGTC CATCACAGTT TTGCATTGGA TAAAGCAGCC
    CCAAAGCCTC CCTTTCAGTC ACACTTCTGT GTTGTGTCTA AACCAAAGGA CTGTATTTTT
    CCATATGCTT TCAAAGAAGT CATTGAGAAA AAGAATGTGA AGGTTGAGGT TGCTGCAACA
    GAAAGAACAC TGCTAGGTTT TTTCCTTGCA AAAGTTCACA AAATTGATCC TGATATCATT
    GTGGGTCATA ATATTTATGG GTTTGAACTG GAAGTACTAC TGCAGAGAAT TAATGTGTGC
    AAAGCTCCTC ACTGGTCCAA GATAGGTCGA CTGAAGCGAT CCAACATGCC AAAGCTTGGG
    GGCCGGAGTG GATTTGGTGA AAGAAATGCT ACCTGTGGTC GAATGATCTG TGATGTGGAA
    ATTTCAGCAA AGGAATTGAT TCGTTGTAAA AGCTACCATC TGTCTGAACT TGTTCAGCAG
    ATTCTAAAAA CTGAAAGGGT TGTAATCCCA ATGGAAAATA TACAAAATAT GTACAGTGAA
    TCTTCTCAAC TGTTATACCT GTTGGAACAC ACCTGGAAAG ATGCCAAGTT CATTTTGCAG
    ATCATGTGTG AGCTAAATGT TCTTCCATTA GCATTGCAGA TCACTAACAT CGCTGGGAAC
    ATTATGTCCA GGACGCTGAT GGGTGGACGA TCCGAGCGTA ACGAGTTCTT GTTGCTTCAT
    GCATTTTACG AAAACAACTA TATTGTGCCT GACAAGCAGA TTTTCAGAAA GCCTCAGCAA
    AAACTGGGAG ATGAAGATGA AGAAATTGAT GGAGATACCA ATAAATACAA GAAAGGACGT
    AAGAAAGCAG CTTATGCTGG AGGCTTGGTT TTGGACCCCA AAGTTGGTTT TTATGATAAG
    TTCATTTTGC TTCTGGACTT CAACAGTCTA TATCCTTCCA TCATTCAGGA ATTTAACATT
    TGTTTTACAA CAGTACAAAG AGTTGCTTCA GAGGCACAGA AAGTTACAGA GGATGGAGAA
    CAAGAACAGA TCCCTGAGTT GCCAGATCCA AGCTTAGAAA TGGGCATTTT GCCCAGAGAG
    ATCCGGAAAC TGGTAGAACG GAGAAAACAA GTCAAACAGC TAATGAAACA GCAAGACTTA
    AATCCAGACC TTATTCTTCA GTATGACATT CGACAGAAGG CTTTGAAGCT CACAGCGAAC
    AGTATGTATG GTTGCCTGGG ATTTTCCTAT AGCAGATTTT ACGCCAAACC ACTGGCTGCC
    TTGGTGACAT ACAAAGGAAG GGAGATTTTG ATGCATACGA AAGAGATGGT ACAAAAGATG
    AATCTTGAAG TTATTTATGG AGATACAGAT TCAATTATGA TAAACACCAA TAGCACCAAT
    CTGGAAGAAG TATTTAAGTT GGGAAACAAG GTAAAAAGTG AAGTGAATAA GTTGTACAAA
    CTGCTTGAAA TAGACATTGA TGGGGTTTTC AAGTCTCTGC TACTGCTGAA AAAAAAGAAG
    TACGCTGCTC TGGTTGTTGA GCCAACGTCG GATGGGAATT ATGTCACCAA ACAGGAGCTC
    AAAGGATTAG ATATAGTTAG AAGAGATTGG TGTGATCTTG CTAAAGACAC TGGAAACTTT
    GTGATTGGCC AGATTCTTTC TGATCAAAGC CGGGACACTA TAGTGGAAAA CATTCAGAAG
    AGGCTGATAG AAATTGGAGA AAATGTGCTA AATGGCAGTG TCCCAGTGAG CCAGTTTGAA
    ATTAACAAGG CATTGACAAA GGATCCCCAG GATTACCCTG ATAAAAAAAG CCTACCTCAT
    GTACATGTTG CCCTCTGGAT AAATTCTCAA GGAGGCAGAA AGGTGAAAGC TGGAGATACT
    GTGTCATATG TCATCTGTCA GGATGGATCA AACCTCACTG CAAGTCAGAG GGCCTATGCG
    CCTGAGCAGC TGCAGAAACA GGATAATCTA ACCATTGACA CCCAGTACTA CCTGGCCCAG
    CAGATCCACC CAGTCGTGGC TCGGATCTGT GAACCAATAG ACGGAATTGA TGCTGTCCTC
    ATTGCAACGT GGTTGGGACT TGACCCCACC CAATTTAGAG TTCATCATTA TCATAAAGAT
    GAAGAGAATG ATGCTCTACT TGGTGGCCCA GCACAGCTCA CTGATGAAGA GAAATACAGG
    GACTGTGAAA GATTCAAATG TCCATGCCCT ACATGTGGAA CTGAGAATAT TTATGATAAT
    GTCTTTGATG GTTCGGGAAC AGATATGGAG CCCAGCTTGT ATCGTTGCAG TAACATCGAT
    TGTAAGGCTT CACCTCTGAC CTTTACAGTA CAACTGAGCA ACAAATTGAT CATGGACATT
    AGACGTTTCA TTAAAAAGTA CTATGATTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.