DUOX1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DUOX1
Entrez Gene ID	53905
Full Name	dual oxidase 1
Synonyms	LNOX1,NOXEF1,THOX1
General protein information	Preferred Names dual oxidase 1 Names dual oxidase 1 NADPH thyroid oxidase 1 flavoprotein NADPH oxidase large NOX 1 long NOX 1 nicotinamide adenine dinucleotide phosphate oxidase thyroid oxidase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q21.1
Summary	The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes proteins encoded by this gene and the similar DUOX2 gene. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. This protein generates hydrogen peroxide and thereby plays a role in the activity of thyroid peroxidase, lactoperoxidase, and in lactoperoxidase-mediated antimicrobial defense at mucosal surfaces. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2012].
Disorder MIM:	606758

mRNA and Protein(s)

mRNA	Protein	Name
NM_175940.2	NP_787954.1	dual oxidase 1 precursor
NM_175940.3	NP_787954.1	dual oxidase 1 precursor
NM_017434.4	NP_059130.2	dual oxidase 1 precursor
NM_017434.5	NP_059130.2	dual oxidase 1 precursor
XM_011521681.2	XP_011519983.1	dual oxidase 1 isoform X1
XM_011521682.1	XP_011519984.1	dual oxidase 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DUOX1	XP_001163196.1
Arabidopsis thaliana (thale cress)	RBOHD	NP_199602.1
Arabidopsis thaliana (thale cress)	AT4G25090	NP_194239.2
Mus musculus (house mouse)	Duox1	NP_001092767.1
Danio rerio (zebrafish)	duox	XP_001919394.4
Arabidopsis thaliana (thale cress)	RBOHA	NP_196356.1
Oryza sativa (rice)	Os01g0360200	NP_001043020.1
Rattus norvegicus (Norway rat)	Duox1	NP_714961.1
Xenopus tropicalis (tropical clawed frog)	duox1	XP_002937936.2
	DUOX1	XP_001111370.1
Canis lupus familiaris (dog)	DUOX1	NP_001003122.1
Gallus gallus (chicken)	DUOX2	XP_425053.4
Homo sapiens (human)	DUOX1	NP_059130.2
Bos taurus (cattle)	DUOX1	NP_001192603.1
Arabidopsis thaliana (thale cress)	RHD2	NP_199919.1

Gene Ontology
Bibliography

Related articles in PubMed

Autophagy regulates DUOX1 localization and superoxide production in airway epithelial cells during chronic IL-13 stimulation.
Dickinson JD, Sweeter JM, Warren KJ, Ahmad IM, De Deken X, Zimmerman MC, Brody SL
Redox biology14272-284(2018 Apr)

Digenic DUOX1 and DUOX2 Mutations in Cases With Congenital Hypothyroidism.
Aycan Z, Cangul H, Muzza M, Bas VN, Fugazzola L, Chatterjee VK, Persani L, Schoenmakers N
The Journal of clinical endocrinology and metabolism102(9)3085-3090(2017 Sep)

Epidermal growth factor-induced hydrogen peroxide production is mediated by dual oxidase 1.
Sirokm?ny G, Pat? A, Zana M, Donk? ?, B?r? A, Nagy P, Geiszt M
Free radical biology & medicine97204-211(2016 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DUOX1 missense mutation plays a role in congenital hypothyroidism.
Title: Digenic DUOX1 and DUOX2 Mutations in Cases With Congenital Hypothyroidism.

We analyzed the expression of NADPH oxidase isoforms and found both A431 and HaCaT cells to express the calcium-sensitive NADPH oxidase, Dual oxidase 1 (Duox1) and its protein partner Duox activator 1 (DuoxA1).Our observations provide evidence for a new signaling paradigm in which changes of intracellular calcium concentration are transformed into redox signals through the calcium-dependent activation of Duox1.
Title: Epidermal growth factor-induced hydrogen peroxide production is mediated by dual oxidase 1.

DUOX1 expression in liver tumors is a potential prognostic tool for patients.
Title: Dual oxidase 1: A predictive tool for the prognosis of hepatocellular carcinoma patients.

Cox multivariate regression analysis demonstrated that DUOX1, GLS2, FBP1 and age were independent risk factors for the prognosis of HCC patients after surgery
Title: The combination of three molecular markers can be a valuable predictive tool for the prognosis of hepatocellular carcinoma patients.

Nox4 and Duox1/Duox2 mediate redox activation of mesenchymal cell migration by PDGF.
Title: Nox4 and Duox1/2 Mediate Redox Activation of Mesenchymal Cell Migration by PDGF.

The following DUOX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DUOX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu68238	XM_011521682.1 Latest version!	Homo sapiens dual oxidase 1 (DUOX1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu10600	NM_017434.5 Latest version!	Homo sapiens dual oxidase 1 (DUOX1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
OHu10600	NM_175940.3 Latest version!	Homo sapiens dual oxidase 1 (DUOX1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
OHu68237	XM_011521681.2 Latest version!	Homo sapiens dual oxidase 1 (DUOX1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OHu10600	NM_175940.2	Homo sapiens dual oxidase 1 (DUOX1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
OHu10600	NM_017434.4	Homo sapiens dual oxidase 1 (DUOX1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DUOX1 ( XM_011521682.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519984.1 Homo sapiens dual oxidase 1 (DUOX1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu68238
Clone ID Related Accession (Same CDS sequence)	XM_011521682.1
Accession Version	XM_011521682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dual oxidase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGATGC ACTGCTGCTG GGCATGGCCT CCCAGATCGC AGAGCGAGAG GACCATGTGT 
TGGTTGAAGA TGTGCGGGGT GAGTCTGAGG CTGTCCCTGC AGGTTGTGAA CTCCTGGCCT 
CTGGGAAGAG GCTCAGCTGG ACTTCCAGAA CCAGATTTCT GGCCTGGGCC ACTGAAGTTT 
TCCCGCACAG ACCACCTGGC CAGCTGCCTG CAGCGGGGCC GGGATCTGGG CCTGCCCTCT 
TACACCAAGG CCAGGGCAGC ACTGGGCTTG TCTCCCATTA CCCGCTGGCA GGACATCAAC 
CCTGCACTCT CCCGGAGCAA TGACACTGTA CTGGAGGCCA CAGCTGCCCT GTACAACCAG 
GACTTATCCT GGCTAGAGCT GCTCCCTGGG GGACTCCTGG AGAGCCACCG GGACCCTGGA 
CCTCTGTTCA GCACCATCGT CCTTGAACAA TTTGTGCGGC TACGGGATGG TGACCGCTAC 
TGGTTTGAGA ACACCAGGAA TGGGCTGTTC TCCAAGAAGG AGATTGAAGA AATCCGAAAT 
ACCACCCTGC AGGACGTGCT GGTCGCTGTT ATCAACATTG ACCCCAGTGC TCTGCAGCCC 
AATGTCTTTG TCTGGCATAA AGGAGACCCC TGTCCGCAGC CGAGACAGCT CAGCACTGAA 
GGCCTGCCAG CGTGTGCTCC CTCTGTTGTT CGTGACTATT TTGAGGGCAG TGGATTTGGC 
TTCGGGGTCA CCATCGGGAC CCTCTGTTGC TTCCCTTTGG TGAGCCTGCT CAGTGCCTGG 
ATTGTTGCCC GGCTCCGGAT GAGAAATTTC AAGAGGCTCC AGGGCCAGGA CCGCCAGAGC 
ATCGTGTCTG AGAAGCTCGT GGGAGGCATG GAAGCTTTGG AATGGCAAGG CCACAAGGAG 
CCCTGCCGGC CCGTGCTTGT GTACCTGCAG CCCGGGCAGA TCCGTGTGGT AGATGGCAGG 
CTCACCGTGC TCCGCACCAT CCAGCTGCAG CCTCCACAGA AGGTCAACTT CGTCCTGTCC 
AGCAACCGTG GACGCCGCAC TCTGCTGCTC AAGATCCCCA AGGAGTATGA CCTGGTGCTG 
CTGTTTAACT TGGAGGAAGA GCGGCAGGCG CTGGTGGAAA ATCTCCGGGG AGCTCTGAAG 
GAGAGCGGGT TGAGCATCCA GGAGTGGGAG CTGCGGGAGC AGGAGCTGAT GAGAGCAGCT 
GTGACACGGG AGCAGCGGAG GCACCTCCTG GAGACCTTTT TCAGGCACCT TTTCTCCCAG 
GTGCTGGACA TCAACCAGGC CGACGCAGGG ACCCTGCCCC TGGACTCCTC CCAGAAGGTG 
CGGGAGGCCC TGACCTGTGA GCTGAGCAGG GCCGAGTTTG CCGAGTCCCT GGGCCTCAAG 
CCCCAGGACA TGTTTGTGGA GTCCATGTTC TCTCTGGCTG ACAAGGATGG CAATGGCTAC 
CTGTCCTTCC GAGAGTTCCT GGACATCCTG GTGGTCTTCA TGAAAGGCTC TCCTGAGGAA 
AAGTCTCGCC TTATGTTCCG CATGTACGAC TTTGATGGGA ATGGCCTCAT TTCCAAGGAT 
GAGTTCATCA GGATGCTGAG ATCCTTCATC GAGATCTCCA ACAACTGCCT GTCCAAGGCC 
CAGCTGGCTG AGGTGGTGGA GTCCATGTTC CGGGAGTCGG GATTCCAGGA CAAGGAGGAA 
CTGACATGGG AAGATTTTCA CTTCATGCTG CGGGACCACA ATAGCGAGCT CCGCTTCACG 
CAGCTCTGTG TCAAAGGGGT GGAGGTGCCT GAAGTCATCA AGGACCTCTG CCGGCGAGCC 
TCCTACATCA GCCAGGATAT GATCTGTCCC TCTCCCAGAG TGAGTGCCCG CTGTTCCCGC 
AGCGACATTG AGACTGAGTT GACACCTCAG AGACTGCAGT GCCCCATGGA CACAGACCCT 
CCCCAGGAGA TTCGGCGGAG GTTTGGCAAG AAGGTAACGT CATTCCAGCC CTTGCTGTTC 
ACTGAGGCGC ACCGAGAGAA GTTCCAACGC AGCTGTCTCC ACCAGACGGT GCAACAGTTC 
AAGCGCTTCA TTGAGAACTA CCGGCGCCAC ATCGGCTGCG TGGCCGTGTT CTACGCCATC 
GCTGGGGGGC TTTTCCTGGA GAGGGCCTAC TACTACGCCT TTGCCGCACA TCACACGGGC 
ATCACAGACA CCACCCGCGT GGGAATCATC CTGTCGCGGG GCACAGCAGC CAGCATCTCT 
TTCATGTTCT CCTACATCTT GCTCACCATG TGCCGCAACC TCATCACCTT CCTGCGAGAA 
ACCTTCCTCA ACCGCTACGT GCCCTTCGAC GCCGCCGTGG ACTTCCATCG CCTCATTGCC 
TCCACCGCCA TCGTCCTCAC AGTCTTACAC AGTGTGGGCC ATGTGGTGAA TGTGTACCTG 
TTCTCCATCA GCCCCCTCAG CGTCCTCTCT TGCCTCTTTC CTGGCCTCTT CCATGATGAT 
GGGTCTGAGC TCCCCCAGAA GTATTACTGG TGGTTCTTCC AGACCGTACC AGGCCTCACG 
GGGGTTGTGC TGCTCCTGAT CCTGGCCATC ATGTATGTCT TTGCCTCCCA CCACTTCCGC 
CGCCGCAGTT TCCGGGGCTT CTGGCTGACC CACCACCTCT ACATCCTGCT CTATGTCCTG 
CTCATCATCC ATGGTAGCTT TGCCCTGATC CAGCTGCCCC GTTTCCACAT CTTCTTCCTG 
GTCCCAGCAA TCATCTATGG GGGCGACAAG CTGGTGAGCC TGAGCCGGAA GAAGGTGGAG 
ATCAGCGTGG TGAAGGCGGA GCTGCTGCCC TCAGGAGTGA CCCACCTGCG GTTCCAGCGG 
CCCCAGGGCT TTGAGTACAA GTCAGGGCAG TGGGTGCGGA TCGCTTGCCT GGCTCTGGGG 
ACCACCGAGT ACCACCCCTT CACACTGACC TCTGCGCCCC ATGAGGACAC GCTTAGCCTG 
CACATCCGGG CAGCAGGGCC CTGGACCACT CGCCTCAGGG AGATCTACTC AGCCCCGACG 
GGTGACAGAT GTGCCAGATA CCCAAAGCTG TACCTTGATG GACCATTTGG AGAGGGCCAC 
CAGGAGTGGC ATAAGTTTGA GGTGTCAGTG TTAGTGGGAG GGGGCATTGG GGTCACCCCT 
TTTGCCTCCA TCCTCAAAGA CCTGGTCTTC AAGTCATCCG TCAGCTGCCA AGTGTTCTGT 
AAGAAGATCT ACTTCATCTG GGTGACGCGG ACCCAGCGTC AGTTTGAGTG GCTGGCTGAC 
ATCATCCGAG AGGTGGAGGA GAATGACCAC CAGGACCTGG TGTCTGTGCA CATCTACATC 
ACCCAGCTGG CTGAGAAGTT CGACCTCAGG ACCACTATGC TGTACATCTG TGAGCGGCAC 
TTCCAGAAGG TTCTGAACCG GAGTCTATTC ACAGGCCTGC GCTCCATCAC CCACTTTGGC 
CGTCCCCCCT TTGAGCCCTT CTTCAACTCC CTGCAGGAGG TCCACCCCCA GGTCCGGAAG 
ATCGGGGTGT TTAGCTGTGG CCCCCCTGGC ATGACCAAGA ATGTGGAAAA GGCCTGTCAG 
CTCATCAACA GGCAGGACCG GACTCACTTC TCCCACCATT ATGAGAACTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519984.1
CDS	20..3613
Translation	MWMHCCWAWPPRSQSERTMCWLKMCGVSLRLSLQVVNSWPLGRGSAGLPEPDFWPGPLKFSRTDHLASCLQRGRDLGLPSYTKARAALGLSPITRWQDINPALSRSNDTVLEATAALYNQDLSWLELLPGGLLESHRDPGPLFSTIVLEQFVRLRDGDRYWFENTRNGLFSKKEIEEIRNTTLQDVLVAVINIDPSALQPNVFVWHKGDPCPQPRQLSTEGLPACAPSVVRDYFEGSGFGFGVTIGTLCCFPLVSLLSAWIVARLRMRNFKRLQGQDRQSIVSEKLVGGMEALEWQGHKEPCRPVLVYLQPGQIRVVDGRLTVLRTIQLQPPQKVNFVLSSNRGRRTLLLKIPKEYDLVLLFNLEEERQALVENLRGALKESGLSIQEWELREQELMRAAVTREQRRHLLETFFRHLFSQVLDINQADAGTLPLDSSQKVREALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHNSELRFTQLCVKGVEVPEVIKDLCRRASYISQDMICPSPRVSARCSRSDIETELTPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSCLHQTVQQFKRFIENYRRHIGCVAVFYAIAGGLFLERAYYYAFAAHHTGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRLIASTAIVLTVLHSVGHVVNVYLFSISPLSVLSCLFPGLFHDDGSELPQKYYWWFFQTVPGLTGVVLLLILAIMYVFASHHFRRRSFRGFWLTHHLYILLYVLLIIHGSFALIQLPRFHIFFLVPAIIYGGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSAPTGDRCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDHQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF

Target ORF information:

RefSeq Version	XM_011521682.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dual oxidase 1 (DUOX1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521682.1

ORF Insert Sequence:

ATGTGGATGC ACTGCTGCTG GGCATGGCCT CCCAGATCGC AGAGCGAGAG GACCATGTGT 
TGGTTGAAGA TGTGCGGGGT GAGTCTGAGG CTGTCCCTGC AGGTTGTGAA CTCCTGGCCT 
CTGGGAAGAG GCTCAGCTGG ACTTCCAGAA CCAGATTTCT GGCCTGGGCC ACTGAAGTTT 
TCCCGCACAG ACCACCTGGC CAGCTGCCTG CAGCGGGGCC GGGATCTGGG CCTGCCCTCT 
TACACCAAGG CCAGGGCAGC ACTGGGCTTG TCTCCCATTA CCCGCTGGCA GGACATCAAC 
CCTGCACTCT CCCGGAGCAA TGACACTGTA CTGGAGGCCA CAGCTGCCCT GTACAACCAG 
GACTTATCCT GGCTAGAGCT GCTCCCTGGG GGACTCCTGG AGAGCCACCG GGACCCTGGA 
CCTCTGTTCA GCACCATCGT CCTTGAACAA TTTGTGCGGC TACGGGATGG TGACCGCTAC 
TGGTTTGAGA ACACCAGGAA TGGGCTGTTC TCCAAGAAGG AGATTGAAGA AATCCGAAAT 
ACCACCCTGC AGGACGTGCT GGTCGCTGTT ATCAACATTG ACCCCAGTGC TCTGCAGCCC 
AATGTCTTTG TCTGGCATAA AGGAGACCCC TGTCCGCAGC CGAGACAGCT CAGCACTGAA 
GGCCTGCCAG CGTGTGCTCC CTCTGTTGTT CGTGACTATT TTGAGGGCAG TGGATTTGGC 
TTCGGGGTCA CCATCGGGAC CCTCTGTTGC TTCCCTTTGG TGAGCCTGCT CAGTGCCTGG 
ATTGTTGCCC GGCTCCGGAT GAGAAATTTC AAGAGGCTCC AGGGCCAGGA CCGCCAGAGC 
ATCGTGTCTG AGAAGCTCGT GGGAGGCATG GAAGCTTTGG AATGGCAAGG CCACAAGGAG 
CCCTGCCGGC CCGTGCTTGT GTACCTGCAG CCCGGGCAGA TCCGTGTGGT AGATGGCAGG 
CTCACCGTGC TCCGCACCAT CCAGCTGCAG CCTCCACAGA AGGTCAACTT CGTCCTGTCC 
AGCAACCGTG GACGCCGCAC TCTGCTGCTC AAGATCCCCA AGGAGTATGA CCTGGTGCTG 
CTGTTTAACT TGGAGGAAGA GCGGCAGGCG CTGGTGGAAA ATCTCCGGGG AGCTCTGAAG 
GAGAGCGGGT TGAGCATCCA GGAGTGGGAG CTGCGGGAGC AGGAGCTGAT GAGAGCAGCT 
GTGACACGGG AGCAGCGGAG GCACCTCCTG GAGACCTTTT TCAGGCACCT TTTCTCCCAG 
GTGCTGGACA TCAACCAGGC CGACGCAGGG ACCCTGCCCC TGGACTCCTC CCAGAAGGTG 
CGGGAGGCCC TGACCTGTGA GCTGAGCAGG GCCGAGTTTG CCGAGTCCCT GGGCCTCAAG 
CCCCAGGACA TGTTTGTGGA GTCCATGTTC TCTCTGGCTG ACAAGGATGG CAATGGCTAC 
CTGTCCTTCC GAGAGTTCCT GGACATCCTG GTGGTCTTCA TGAAAGGCTC TCCTGAGGAA 
AAGTCTCGCC TTATGTTCCG CATGTACGAC TTTGATGGGA ATGGCCTCAT TTCCAAGGAT 
GAGTTCATCA GGATGCTGAG ATCCTTCATC GAGATCTCCA ACAACTGCCT GTCCAAGGCC 
CAGCTGGCTG AGGTGGTGGA GTCCATGTTC CGGGAGTCGG GATTCCAGGA CAAGGAGGAA 
CTGACATGGG AAGATTTTCA CTTCATGCTG CGGGACCACA ATAGCGAGCT CCGCTTCACG 
CAGCTCTGTG TCAAAGGGGT GGAGGTGCCT GAAGTCATCA AGGACCTCTG CCGGCGAGCC 
TCCTACATCA GCCAGGATAT GATCTGTCCC TCTCCCAGAG TGAGTGCCCG CTGTTCCCGC 
AGCGACATTG AGACTGAGTT GACACCTCAG AGACTGCAGT GCCCCATGGA CACAGACCCT 
CCCCAGGAGA TTCGGCGGAG GTTTGGCAAG AAGGTAACGT CATTCCAGCC CTTGCTGTTC 
ACTGAGGCGC ACCGAGAGAA GTTCCAACGC AGCTGTCTCC ACCAGACGGT GCAACAGTTC 
AAGCGCTTCA TTGAGAACTA CCGGCGCCAC ATCGGCTGCG TGGCCGTGTT CTACGCCATC 
GCTGGGGGGC TTTTCCTGGA GAGGGCCTAC TACTACGCCT TTGCCGCACA TCACACGGGC 
ATCACAGACA CCACCCGCGT GGGAATCATC CTGTCGCGGG GCACAGCAGC CAGCATCTCT 
TTCATGTTCT CCTACATCTT GCTCACCATG TGCCGCAACC TCATCACCTT CCTGCGAGAA 
ACCTTCCTCA ACCGCTACGT GCCCTTCGAC GCCGCCGTGG ACTTCCATCG CCTCATTGCC 
TCCACCGCCA TCGTCCTCAC AGTCTTACAC AGTGTGGGCC ATGTGGTGAA TGTGTACCTG 
TTCTCCATCA GCCCCCTCAG CGTCCTCTCT TGCCTCTTTC CTGGCCTCTT CCATGATGAT 
GGGTCTGAGC TCCCCCAGAA GTATTACTGG TGGTTCTTCC AGACCGTACC AGGCCTCACG 
GGGGTTGTGC TGCTCCTGAT CCTGGCCATC ATGTATGTCT TTGCCTCCCA CCACTTCCGC 
CGCCGCAGTT TCCGGGGCTT CTGGCTGACC CACCACCTCT ACATCCTGCT CTATGTCCTG 
CTCATCATCC ATGGTAGCTT TGCCCTGATC CAGCTGCCCC GTTTCCACAT CTTCTTCCTG 
GTCCCAGCAA TCATCTATGG GGGCGACAAG CTGGTGAGCC TGAGCCGGAA GAAGGTGGAG 
ATCAGCGTGG TGAAGGCGGA GCTGCTGCCC TCAGGAGTGA CCCACCTGCG GTTCCAGCGG 
CCCCAGGGCT TTGAGTACAA GTCAGGGCAG TGGGTGCGGA TCGCTTGCCT GGCTCTGGGG 
ACCACCGAGT ACCACCCCTT CACACTGACC TCTGCGCCCC ATGAGGACAC GCTTAGCCTG 
CACATCCGGG CAGCAGGGCC CTGGACCACT CGCCTCAGGG AGATCTACTC AGCCCCGACG 
GGTGACAGAT GTGCCAGATA CCCAAAGCTG TACCTTGATG GACCATTTGG AGAGGGCCAC 
CAGGAGTGGC ATAAGTTTGA GGTGTCAGTG TTAGTGGGAG GGGGCATTGG GGTCACCCCT 
TTTGCCTCCA TCCTCAAAGA CCTGGTCTTC AAGTCATCCG TCAGCTGCCA AGTGTTCTGT 
AAGAAGATCT ACTTCATCTG GGTGACGCGG ACCCAGCGTC AGTTTGAGTG GCTGGCTGAC 
ATCATCCGAG AGGTGGAGGA GAATGACCAC CAGGACCTGG TGTCTGTGCA CATCTACATC 
ACCCAGCTGG CTGAGAAGTT CGACCTCAGG ACCACTATGC TGTACATCTG TGAGCGGCAC 
TTCCAGAAGG TTCTGAACCG GAGTCTATTC ACAGGCCTGC GCTCCATCAC CCACTTTGGC 
CGTCCCCCCT TTGAGCCCTT CTTCAACTCC CTGCAGGAGG TCCACCCCCA GGTCCGGAAG 
ATCGGGGTGT TTAGCTGTGG CCCCCCTGGC ATGACCAAGA ATGTGGAAAA GGCCTGTCAG 
CTCATCAACA GGCAGGACCG GACTCACTTC TCCCACCATT ATGAGAACTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DUOX1 ( NM_175940.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_787954.1 Homo sapiens dual oxidase 1 (DUOX1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10600
Clone ID Related Accession (Same CDS sequence)	NM_175940.2 , NM_175940.3 , NM_017434.4 , NM_017434.5
Accession Version	NM_175940.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-08
Organism	Homo sapiens(human)
Product	dual oxidase 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF213465.1, AF230495.1, AC051619.7 and CB852814.1. On Sep 11, 2013 this sequence version replaced NM_175940.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF213465.1, AK075268.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTTCT GCCTGGCTCT AGCATGGACA CTTCTGGTTG GGGCATGGAC CCCTCTGGGA 
GCTCAGAACC CCATTTCGTG GGAGGTGCAG CGATTTGATG GGTGGTACAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCCCGG CTGCAGCGCC TGGTCCCAGC CAGCTATGCA 
GATGGCGTGT ACCAGCCCTT GGGAGAACCC CACCTGCCCA ACCCCCGAGA CCTTAGCAAC 
ACCATCTCAA GGGGCCCTGC AGGGCTGGCC TCCCTGAGAA ACCGCACAGT GTTGGGGGTC 
TTCTTTGGCT ATCACGTGCT TTCAGACCTG GTGAGCGTGG AAACTCCCGG CTGCCCCGCC 
GAGTTCCTCA ACATTCGCAT CCCGCCCGGA GACCCCATGT TCGACCCCGA CCAGCGCGGG 
GACGTGGTGC TGCCCTTCCA GAGAAGCCGC TGGGACCCCG AGACCGGACG GAGTCCCAGC 
AATCCCCGGG ACCCGGCCAA CCAGGTGACG GGCTGGCTGG ACGGCAGCGC CATCTATGGT 
TCCTCGCATT CCTGGAGCGA CGCGCTGCGG AGCTTCTCCA GGGGACAGCT GGCGTCGGGG 
CCCGACCCCG CTTTTCCCCG AGACTCGCAG AACCCCCTGC TCATGTGGGC GGCGCCCGAC 
CCCGCCACCG GGCAGAACGG GCCCCGGGGG CTGTACGCCT TCGGGGCAGA GAGAGGGAAC 
CGGGAACCCT TCCTGCAGGC GCTGGGCCTG CTCTGGTTCC GCTACCACAA CCTGTGGGCG 
CAGAGGCTGG CCCGCCAGCA CCCAGACTGG GAGGACGAGG AGCTGTTCCA GCACGCACGC 
AAGAGGGTCA TCGCCACCTA CCAGAACATC GCTGTGTATG AGTGGCTGCC CAGCTTCCTG 
CAGAAAACAC TCCCGGAGTA TACAGGATAC CGGCCATTTC TGGACCCCAG CATCTCCTCA 
GAGTTCGTGG CGGCCTCTGA GCAGTTCCTG TCCACCATGG TGCCCCCTGG CGTCTACATG 
AGAAATGCCA GCTGCCACTT CCAGGGGGTC ATCAATCGGA ACTCAAGTGT CTCCAGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGCCGT GAGCACCCAA GCCTACAAAG TGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATCGCAG AGCGAGAGGA CCATGTGTTG 
GTTGAAGATG TGCGGGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGCAC AGACCACCTG 
GCCAGCTGCC TGCAGCGGGG CCGGGATCTG GGCCTGCCCT CTTACACCAA GGCCAGGGCA 
GCACTGGGCT TGTCTCCCAT TACCCGCTGG CAGGACATCA ACCCTGCACT CTCCCGGAGC 
AATGACACTG TACTGGAGGC CACAGCTGCC CTGTACAACC AGGACTTATC CTGGCTAGAG 
CTGCTCCCTG GGGGACTCCT GGAGAGCCAC CGGGACCCTG GACCTCTGTT CAGCACCATC 
GTCCTTGAAC AATTTGTGCG GCTACGGGAT GGTGACCGCT ACTGGTTTGA GAACACCAGG 
AATGGGCTGT TCTCCAAGAA GGAGATTGAA GAAATCCGAA ATACCACCCT GCAGGACGTG 
CTGGTCGCTG TTATCAACAT TGACCCCAGT GCTCTGCAGC CCAATGTCTT TGTCTGGCAT 
AAAGGAGACC CCTGTCCGCA GCCGAGACAG CTCAGCACTG AAGGCCTGCC AGCGTGTGCT 
CCCTCTGTTG TTCGTGACTA TTTTGAGGGC AGTGGATTTG GCTTCGGGGT CACCATCGGG 
ACCCTCTGTT GCTTCCCTTT GGTGAGCCTG CTCAGTGCCT GGATTGTTGC CCGGCTCCGG 
ATGAGAAATT TCAAGAGGCT CCAGGGCCAG GACCGCCAGA GCATCGTGTC TGAGAAGCTC 
GTGGGAGGCA TGGAAGCTTT GGAATGGCAA GGCCACAAGG AGCCCTGCCG GCCCGTGCTT 
GTGTACCTGC AGCCCGGGCA GATCCGTGTG GTAGATGGCA GGCTCACCGT GCTCCGCACC 
ATCCAGCTGC AGCCTCCACA GAAGGTCAAC TTCGTCCTGT CCAGCAACCG TGGACGCCGC 
ACTCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTGC TGCTGTTTAA CTTGGAGGAA 
GAGCGGCAGG CGCTGGTGGA AAATCTCCGG GGAGCTCTGA AGGAGAGCGG GTTGAGCATC 
CAGGAGTGGG AGCTGCGGGA GCAGGAGCTG ATGAGAGCAG CTGTGACACG GGAGCAGCGG 
AGGCACCTCC TGGAGACCTT TTTCAGGCAC CTTTTCTCCC AGGTGCTGGA CATCAACCAG 
GCCGACGCAG GGACCCTGCC CCTGGACTCC TCCCAGAAGG TGCGGGAGGC CCTGACCTGT 
GAGCTGAGCA GGGCCGAGTT TGCCGAGTCC CTGGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TCTCTCTGGC TGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGAGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCTCCTGAGG AAAAGTCTCG CCTTATGTTC 
CGCATGTACG ACTTTGATGG GAATGGCCTC ATTTCCAAGG ATGAGTTCAT CAGGATGCTG 
AGATCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTG 
GAGTCCATGT TCCGGGAGTC GGGATTCCAG GACAAGGAGG AACTGACATG GGAAGATTTT 
CACTTCATGC TGCGGGACCA CAATAGCGAG CTCCGCTTCA CGCAGCTCTG TGTCAAAGGG 
GTGGAGGTGC CTGAAGTCAT CAAGGACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAT 
ATGATCTGTC CCTCTCCCAG AGTGAGTGCC CGCTGTTCCC GCAGCGACAT TGAGACTGAG 
TTGACACCTC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCCCAGGA GATTCGGCGG 
AGGTTTGGCA AGAAGGTAAC GTCATTCCAG CCCTTGCTGT TCACTGAGGC GCACCGAGAG 
AAGTTCCAAC GCAGCTGTCT CCACCAGACG GTGCAACAGT TCAAGCGCTT CATTGAGAAC 
TACCGGCGCC ACATCGGCTG CGTGGCCGTG TTCTACGCCA TCGCTGGGGG GCTTTTCCTG 
GAGAGGGCCT ACTACTACGC CTTTGCCGCA CATCACACGG GCATCACAGA CACCACCCGC 
GTGGGAATCA TCCTGTCGCG GGGCACAGCA GCCAGCATCT CTTTCATGTT CTCCTACATC 
TTGCTCACCA TGTGCCGCAA CCTCATCACC TTCCTGCGAG AAACCTTCCT CAACCGCTAC 
GTGCCCTTCG ACGCCGCCGT GGACTTCCAT CGCCTCATTG CCTCCACCGC CATCGTCCTC 
ACAGTCTTAC ACAGTGTGGG CCATGTGGTG AATGTGTACC TGTTCTCCAT CAGCCCCCTC 
AGCGTCCTCT CTTGCCTCTT TCCTGGCCTC TTCCATGATG ATGGGTCTGA GCTCCCCCAG 
AAGTATTACT GGTGGTTCTT CCAGACCGTA CCAGGCCTCA CGGGGGTTGT GCTGCTCCTG 
ATCCTGGCCA TCATGTATGT CTTTGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCACCACCT CTACATCCTG CTCTATGTCC TGCTCATCAT CCATGGTAGC 
TTTGCCCTGA TCCAGCTGCC CCGTTTCCAC ATCTTCTTCC TGGTCCCAGC AATCATCTAT 
GGGGGCGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCG 
GAGCTGCTGC CCTCAGGAGT GACCCACCTG CGGTTCCAGC GGCCCCAGGG CTTTGAGTAC 
AAGTCAGGGC AGTGGGTGCG GATCGCTTGC CTGGCTCTGG GGACCACCGA GTACCACCCC 
TTCACACTGA CCTCTGCGCC CCATGAGGAC ACGCTTAGCC TGCACATCCG GGCAGCAGGG 
CCCTGGACCA CTCGCCTCAG GGAGATCTAC TCAGCCCCGA CGGGTGACAG ATGTGCCAGA 
TACCCAAAGC TGTACCTTGA TGGACCATTT GGAGAGGGCC ACCAGGAGTG GCATAAGTTT 
GAGGTGTCAG TGTTAGTGGG AGGGGGCATT GGGGTCACCC CTTTTGCCTC CATCCTCAAA 
GACCTGGTCT TCAAGTCATC CGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACGC GGACCCAGCG TCAGTTTGAG TGGCTGGCTG ACATCATCCG AGAGGTGGAG 
GAGAATGACC ACCAGGACCT GGTGTCTGTG CACATCTACA TCACCCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACTAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTTCTGAAC 
CGGAGTCTAT TCACAGGCCT GCGCTCCATC ACCCACTTTG GCCGTCCCCC CTTTGAGCCC 
TTCTTCAACT CCCTGCAGGA GGTCCACCCC CAGGTCCGGA AGATCGGGGT GTTTAGCTGT 
GGCCCCCCTG GCATGACCAA GAATGTGGAA AAGGCCTGTC AGCTCATCAA CAGGCAGGAC 
CGGACTCACT TCTCCCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787954.1
CDS	155..4810
Translation	MGFCLALAWTLLVGAWTPLGAQNPISWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLGEPHLPNPRDLSNTISRGPAGLASLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIRIPPGDPMFDPDQRGDVVLPFQRSRWDPETGRSPSNPRDPANQVTGWLDGSAIYGSSHSWSDALRSFSRGQLASGPDPAFPRDSQNPLLMWAAPDPATGQNGPRGLYAFGAERGNREPFLQALGLLWFRYHNLWAQRLARQHPDWEDEELFQHARKRVIATYQNIAVYEWLPSFLQKTLPEYTGYRPFLDPSISSEFVAASEQFLSTMVPPGVYMRNASCHFQGVINRNSSVSRALRVCNSYWSREHPSLQSAEDVDALLLGMASQIAEREDHVLVEDVRDFWPGPLKFSRTDHLASCLQRGRDLGLPSYTKARAALGLSPITRWQDINPALSRSNDTVLEATAALYNQDLSWLELLPGGLLESHRDPGPLFSTIVLEQFVRLRDGDRYWFENTRNGLFSKKEIEEIRNTTLQDVLVAVINIDPSALQPNVFVWHKGDPCPQPRQLSTEGLPACAPSVVRDYFEGSGFGFGVTIGTLCCFPLVSLLSAWIVARLRMRNFKRLQGQDRQSIVSEKLVGGMEALEWQGHKEPCRPVLVYLQPGQIRVVDGRLTVLRTIQLQPPQKVNFVLSSNRGRRTLLLKIPKEYDLVLLFNLEEERQALVENLRGALKESGLSIQEWELREQELMRAAVTREQRRHLLETFFRHLFSQVLDINQADAGTLPLDSSQKVREALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHNSELRFTQLCVKGVEVPEVIKDLCRRASYISQDMICPSPRVSARCSRSDIETELTPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSCLHQTVQQFKRFIENYRRHIGCVAVFYAIAGGLFLERAYYYAFAAHHTGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRLIASTAIVLTVLHSVGHVVNVYLFSISPLSVLSCLFPGLFHDDGSELPQKYYWWFFQTVPGLTGVVLLLILAIMYVFASHHFRRRSFRGFWLTHHLYILLYVLLIIHGSFALIQLPRFHIFFLVPAIIYGGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSAPTGDRCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDHQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF

Target ORF information:

RefSeq Version	NM_175940.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dual oxidase 1 (DUOX1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175940.2

ORF Insert Sequence:

ATGGGCTTCT GCCTGGCTCT AGCATGGACA CTTCTGGTTG GGGCATGGAC CCCTCTGGGA 
GCTCAGAACC CCATTTCGTG GGAGGTGCAG CGATTTGATG GGTGGTACAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCCCGG CTGCAGCGCC TGGTCCCAGC CAGCTATGCA 
GATGGCGTGT ACCAGCCCTT GGGAGAACCC CACCTGCCCA ACCCCCGAGA CCTTAGCAAC 
ACCATCTCAA GGGGCCCTGC AGGGCTGGCC TCCCTGAGAA ACCGCACAGT GTTGGGGGTC 
TTCTTTGGCT ATCACGTGCT TTCAGACCTG GTGAGCGTGG AAACTCCCGG CTGCCCCGCC 
GAGTTCCTCA ACATTCGCAT CCCGCCCGGA GACCCCATGT TCGACCCCGA CCAGCGCGGG 
GACGTGGTGC TGCCCTTCCA GAGAAGCCGC TGGGACCCCG AGACCGGACG GAGTCCCAGC 
AATCCCCGGG ACCCGGCCAA CCAGGTGACG GGCTGGCTGG ACGGCAGCGC CATCTATGGT 
TCCTCGCATT CCTGGAGCGA CGCGCTGCGG AGCTTCTCCA GGGGACAGCT GGCGTCGGGG 
CCCGACCCCG CTTTTCCCCG AGACTCGCAG AACCCCCTGC TCATGTGGGC GGCGCCCGAC 
CCCGCCACCG GGCAGAACGG GCCCCGGGGG CTGTACGCCT TCGGGGCAGA GAGAGGGAAC 
CGGGAACCCT TCCTGCAGGC GCTGGGCCTG CTCTGGTTCC GCTACCACAA CCTGTGGGCG 
CAGAGGCTGG CCCGCCAGCA CCCAGACTGG GAGGACGAGG AGCTGTTCCA GCACGCACGC 
AAGAGGGTCA TCGCCACCTA CCAGAACATC GCTGTGTATG AGTGGCTGCC CAGCTTCCTG 
CAGAAAACAC TCCCGGAGTA TACAGGATAC CGGCCATTTC TGGACCCCAG CATCTCCTCA 
GAGTTCGTGG CGGCCTCTGA GCAGTTCCTG TCCACCATGG TGCCCCCTGG CGTCTACATG 
AGAAATGCCA GCTGCCACTT CCAGGGGGTC ATCAATCGGA ACTCAAGTGT CTCCAGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGCCGT GAGCACCCAA GCCTACAAAG TGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATCGCAG AGCGAGAGGA CCATGTGTTG 
GTTGAAGATG TGCGGGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGCAC AGACCACCTG 
GCCAGCTGCC TGCAGCGGGG CCGGGATCTG GGCCTGCCCT CTTACACCAA GGCCAGGGCA 
GCACTGGGCT TGTCTCCCAT TACCCGCTGG CAGGACATCA ACCCTGCACT CTCCCGGAGC 
AATGACACTG TACTGGAGGC CACAGCTGCC CTGTACAACC AGGACTTATC CTGGCTAGAG 
CTGCTCCCTG GGGGACTCCT GGAGAGCCAC CGGGACCCTG GACCTCTGTT CAGCACCATC 
GTCCTTGAAC AATTTGTGCG GCTACGGGAT GGTGACCGCT ACTGGTTTGA GAACACCAGG 
AATGGGCTGT TCTCCAAGAA GGAGATTGAA GAAATCCGAA ATACCACCCT GCAGGACGTG 
CTGGTCGCTG TTATCAACAT TGACCCCAGT GCTCTGCAGC CCAATGTCTT TGTCTGGCAT 
AAAGGAGACC CCTGTCCGCA GCCGAGACAG CTCAGCACTG AAGGCCTGCC AGCGTGTGCT 
CCCTCTGTTG TTCGTGACTA TTTTGAGGGC AGTGGATTTG GCTTCGGGGT CACCATCGGG 
ACCCTCTGTT GCTTCCCTTT GGTGAGCCTG CTCAGTGCCT GGATTGTTGC CCGGCTCCGG 
ATGAGAAATT TCAAGAGGCT CCAGGGCCAG GACCGCCAGA GCATCGTGTC TGAGAAGCTC 
GTGGGAGGCA TGGAAGCTTT GGAATGGCAA GGCCACAAGG AGCCCTGCCG GCCCGTGCTT 
GTGTACCTGC AGCCCGGGCA GATCCGTGTG GTAGATGGCA GGCTCACCGT GCTCCGCACC 
ATCCAGCTGC AGCCTCCACA GAAGGTCAAC TTCGTCCTGT CCAGCAACCG TGGACGCCGC 
ACTCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTGC TGCTGTTTAA CTTGGAGGAA 
GAGCGGCAGG CGCTGGTGGA AAATCTCCGG GGAGCTCTGA AGGAGAGCGG GTTGAGCATC 
CAGGAGTGGG AGCTGCGGGA GCAGGAGCTG ATGAGAGCAG CTGTGACACG GGAGCAGCGG 
AGGCACCTCC TGGAGACCTT TTTCAGGCAC CTTTTCTCCC AGGTGCTGGA CATCAACCAG 
GCCGACGCAG GGACCCTGCC CCTGGACTCC TCCCAGAAGG TGCGGGAGGC CCTGACCTGT 
GAGCTGAGCA GGGCCGAGTT TGCCGAGTCC CTGGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TCTCTCTGGC TGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGAGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCTCCTGAGG AAAAGTCTCG CCTTATGTTC 
CGCATGTACG ACTTTGATGG GAATGGCCTC ATTTCCAAGG ATGAGTTCAT CAGGATGCTG 
AGATCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTG 
GAGTCCATGT TCCGGGAGTC GGGATTCCAG GACAAGGAGG AACTGACATG GGAAGATTTT 
CACTTCATGC TGCGGGACCA CAATAGCGAG CTCCGCTTCA CGCAGCTCTG TGTCAAAGGG 
GTGGAGGTGC CTGAAGTCAT CAAGGACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAT 
ATGATCTGTC CCTCTCCCAG AGTGAGTGCC CGCTGTTCCC GCAGCGACAT TGAGACTGAG 
TTGACACCTC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCCCAGGA GATTCGGCGG 
AGGTTTGGCA AGAAGGTAAC GTCATTCCAG CCCTTGCTGT TCACTGAGGC GCACCGAGAG 
AAGTTCCAAC GCAGCTGTCT CCACCAGACG GTGCAACAGT TCAAGCGCTT CATTGAGAAC 
TACCGGCGCC ACATCGGCTG CGTGGCCGTG TTCTACGCCA TCGCTGGGGG GCTTTTCCTG 
GAGAGGGCCT ACTACTACGC CTTTGCCGCA CATCACACGG GCATCACAGA CACCACCCGC 
GTGGGAATCA TCCTGTCGCG GGGCACAGCA GCCAGCATCT CTTTCATGTT CTCCTACATC 
TTGCTCACCA TGTGCCGCAA CCTCATCACC TTCCTGCGAG AAACCTTCCT CAACCGCTAC 
GTGCCCTTCG ACGCCGCCGT GGACTTCCAT CGCCTCATTG CCTCCACCGC CATCGTCCTC 
ACAGTCTTAC ACAGTGTGGG CCATGTGGTG AATGTGTACC TGTTCTCCAT CAGCCCCCTC 
AGCGTCCTCT CTTGCCTCTT TCCTGGCCTC TTCCATGATG ATGGGTCTGA GCTCCCCCAG 
AAGTATTACT GGTGGTTCTT CCAGACCGTA CCAGGCCTCA CGGGGGTTGT GCTGCTCCTG 
ATCCTGGCCA TCATGTATGT CTTTGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCACCACCT CTACATCCTG CTCTATGTCC TGCTCATCAT CCATGGTAGC 
TTTGCCCTGA TCCAGCTGCC CCGTTTCCAC ATCTTCTTCC TGGTCCCAGC AATCATCTAT 
GGGGGCGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCG 
GAGCTGCTGC CCTCAGGAGT GACCCACCTG CGGTTCCAGC GGCCCCAGGG CTTTGAGTAC 
AAGTCAGGGC AGTGGGTGCG GATCGCTTGC CTGGCTCTGG GGACCACCGA GTACCACCCC 
TTCACACTGA CCTCTGCGCC CCATGAGGAC ACGCTTAGCC TGCACATCCG GGCAGCAGGG 
CCCTGGACCA CTCGCCTCAG GGAGATCTAC TCAGCCCCGA CGGGTGACAG ATGTGCCAGA 
TACCCAAAGC TGTACCTTGA TGGACCATTT GGAGAGGGCC ACCAGGAGTG GCATAAGTTT 
GAGGTGTCAG TGTTAGTGGG AGGGGGCATT GGGGTCACCC CTTTTGCCTC CATCCTCAAA 
GACCTGGTCT TCAAGTCATC CGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACGC GGACCCAGCG TCAGTTTGAG TGGCTGGCTG ACATCATCCG AGAGGTGGAG 
GAGAATGACC ACCAGGACCT GGTGTCTGTG CACATCTACA TCACCCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACTAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTTCTGAAC 
CGGAGTCTAT TCACAGGCCT GCGCTCCATC ACCCACTTTG GCCGTCCCCC CTTTGAGCCC 
TTCTTCAACT CCCTGCAGGA GGTCCACCCC CAGGTCCGGA AGATCGGGGT GTTTAGCTGT 
GGCCCCCCTG GCATGACCAA GAATGTGGAA AAGGCCTGTC AGCTCATCAA CAGGCAGGAC 
CGGACTCACT TCTCCCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DUOX1 ( NM_175940.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_787954.1 Homo sapiens dual oxidase 1 (DUOX1), transcript variant 2, mRNA.

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CloneID	OHu10600
Clone ID Related Accession (Same CDS sequence)	NM_175940.2 , NM_175940.3 , NM_017434.4 , NM_017434.5
Accession Version	NM_175940.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	dual oxidase 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF213465.1, AF230495.1, AC051619.7 and CB852814.1. On Nov 23, 2018 this sequence version replaced NM_175940.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF213465.1, BC114628.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142363 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000389037.7/ ENSP00000373689.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCTTCT GCCTGGCTCT AGCATGGACA CTTCTGGTTG GGGCATGGAC CCCTCTGGGA 
GCTCAGAACC CCATTTCGTG GGAGGTGCAG CGATTTGATG GGTGGTACAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCCCGG CTGCAGCGCC TGGTCCCAGC CAGCTATGCA 
GATGGCGTGT ACCAGCCCTT GGGAGAACCC CACCTGCCCA ACCCCCGAGA CCTTAGCAAC 
ACCATCTCAA GGGGCCCTGC AGGGCTGGCC TCCCTGAGAA ACCGCACAGT GTTGGGGGTC 
TTCTTTGGCT ATCACGTGCT TTCAGACCTG GTGAGCGTGG AAACTCCCGG CTGCCCCGCC 
GAGTTCCTCA ACATTCGCAT CCCGCCCGGA GACCCCATGT TCGACCCCGA CCAGCGCGGG 
GACGTGGTGC TGCCCTTCCA GAGAAGCCGC TGGGACCCCG AGACCGGACG GAGTCCCAGC 
AATCCCCGGG ACCCGGCCAA CCAGGTGACG GGCTGGCTGG ACGGCAGCGC CATCTATGGT 
TCCTCGCATT CCTGGAGCGA CGCGCTGCGG AGCTTCTCCA GGGGACAGCT GGCGTCGGGG 
CCCGACCCCG CTTTTCCCCG AGACTCGCAG AACCCCCTGC TCATGTGGGC GGCGCCCGAC 
CCCGCCACCG GGCAGAACGG GCCCCGGGGG CTGTACGCCT TCGGGGCAGA GAGAGGGAAC 
CGGGAACCCT TCCTGCAGGC GCTGGGCCTG CTCTGGTTCC GCTACCACAA CCTGTGGGCG 
CAGAGGCTGG CCCGCCAGCA CCCAGACTGG GAGGACGAGG AGCTGTTCCA GCACGCACGC 
AAGAGGGTCA TCGCCACCTA CCAGAACATC GCTGTGTATG AGTGGCTGCC CAGCTTCCTG 
CAGAAAACAC TCCCGGAGTA TACAGGATAC CGGCCATTTC TGGACCCCAG CATCTCCTCA 
GAGTTCGTGG CGGCCTCTGA GCAGTTCCTG TCCACCATGG TGCCCCCTGG CGTCTACATG 
AGAAATGCCA GCTGCCACTT CCAGGGGGTC ATCAATCGGA ACTCAAGTGT CTCCAGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGCCGT GAGCACCCAA GCCTACAAAG TGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATCGCAG AGCGAGAGGA CCATGTGTTG 
GTTGAAGATG TGCGGGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGCAC AGACCACCTG 
GCCAGCTGCC TGCAGCGGGG CCGGGATCTG GGCCTGCCCT CTTACACCAA GGCCAGGGCA 
GCACTGGGCT TGTCTCCCAT TACCCGCTGG CAGGACATCA ACCCTGCACT CTCCCGGAGC 
AATGACACTG TACTGGAGGC CACAGCTGCC CTGTACAACC AGGACTTATC CTGGCTAGAG 
CTGCTCCCTG GGGGACTCCT GGAGAGCCAC CGGGACCCTG GACCTCTGTT CAGCACCATC 
GTCCTTGAAC AATTTGTGCG GCTACGGGAT GGTGACCGCT ACTGGTTTGA GAACACCAGG 
AATGGGCTGT TCTCCAAGAA GGAGATTGAA GAAATCCGAA ATACCACCCT GCAGGACGTG 
CTGGTCGCTG TTATCAACAT TGACCCCAGT GCTCTGCAGC CCAATGTCTT TGTCTGGCAT 
AAAGGAGACC CCTGTCCGCA GCCGAGACAG CTCAGCACTG AAGGCCTGCC AGCGTGTGCT 
CCCTCTGTTG TTCGTGACTA TTTTGAGGGC AGTGGATTTG GCTTCGGGGT CACCATCGGG 
ACCCTCTGTT GCTTCCCTTT GGTGAGCCTG CTCAGTGCCT GGATTGTTGC CCGGCTCCGG 
ATGAGAAATT TCAAGAGGCT CCAGGGCCAG GACCGCCAGA GCATCGTGTC TGAGAAGCTC 
GTGGGAGGCA TGGAAGCTTT GGAATGGCAA GGCCACAAGG AGCCCTGCCG GCCCGTGCTT 
GTGTACCTGC AGCCCGGGCA GATCCGTGTG GTAGATGGCA GGCTCACCGT GCTCCGCACC 
ATCCAGCTGC AGCCTCCACA GAAGGTCAAC TTCGTCCTGT CCAGCAACCG TGGACGCCGC 
ACTCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTGC TGCTGTTTAA CTTGGAGGAA 
GAGCGGCAGG CGCTGGTGGA AAATCTCCGG GGAGCTCTGA AGGAGAGCGG GTTGAGCATC 
CAGGAGTGGG AGCTGCGGGA GCAGGAGCTG ATGAGAGCAG CTGTGACACG GGAGCAGCGG 
AGGCACCTCC TGGAGACCTT TTTCAGGCAC CTTTTCTCCC AGGTGCTGGA CATCAACCAG 
GCCGACGCAG GGACCCTGCC CCTGGACTCC TCCCAGAAGG TGCGGGAGGC CCTGACCTGT 
GAGCTGAGCA GGGCCGAGTT TGCCGAGTCC CTGGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TCTCTCTGGC TGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGAGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCTCCTGAGG AAAAGTCTCG CCTTATGTTC 
CGCATGTACG ACTTTGATGG GAATGGCCTC ATTTCCAAGG ATGAGTTCAT CAGGATGCTG 
AGATCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTG 
GAGTCCATGT TCCGGGAGTC GGGATTCCAG GACAAGGAGG AACTGACATG GGAAGATTTT 
CACTTCATGC TGCGGGACCA CAATAGCGAG CTCCGCTTCA CGCAGCTCTG TGTCAAAGGG 
GTGGAGGTGC CTGAAGTCAT CAAGGACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAT 
ATGATCTGTC CCTCTCCCAG AGTGAGTGCC CGCTGTTCCC GCAGCGACAT TGAGACTGAG 
TTGACACCTC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCCCAGGA GATTCGGCGG 
AGGTTTGGCA AGAAGGTAAC GTCATTCCAG CCCTTGCTGT TCACTGAGGC GCACCGAGAG 
AAGTTCCAAC GCAGCTGTCT CCACCAGACG GTGCAACAGT TCAAGCGCTT CATTGAGAAC 
TACCGGCGCC ACATCGGCTG CGTGGCCGTG TTCTACGCCA TCGCTGGGGG GCTTTTCCTG 
GAGAGGGCCT ACTACTACGC CTTTGCCGCA CATCACACGG GCATCACAGA CACCACCCGC 
GTGGGAATCA TCCTGTCGCG GGGCACAGCA GCCAGCATCT CTTTCATGTT CTCCTACATC 
TTGCTCACCA TGTGCCGCAA CCTCATCACC TTCCTGCGAG AAACCTTCCT CAACCGCTAC 
GTGCCCTTCG ACGCCGCCGT GGACTTCCAT CGCCTCATTG CCTCCACCGC CATCGTCCTC 
ACAGTCTTAC ACAGTGTGGG CCATGTGGTG AATGTGTACC TGTTCTCCAT CAGCCCCCTC 
AGCGTCCTCT CTTGCCTCTT TCCTGGCCTC TTCCATGATG ATGGGTCTGA GCTCCCCCAG 
AAGTATTACT GGTGGTTCTT CCAGACCGTA CCAGGCCTCA CGGGGGTTGT GCTGCTCCTG 
ATCCTGGCCA TCATGTATGT CTTTGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCACCACCT CTACATCCTG CTCTATGTCC TGCTCATCAT CCATGGTAGC 
TTTGCCCTGA TCCAGCTGCC CCGTTTCCAC ATCTTCTTCC TGGTCCCAGC AATCATCTAT 
GGGGGCGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCG 
GAGCTGCTGC CCTCAGGAGT GACCCACCTG CGGTTCCAGC GGCCCCAGGG CTTTGAGTAC 
AAGTCAGGGC AGTGGGTGCG GATCGCTTGC CTGGCTCTGG GGACCACCGA GTACCACCCC 
TTCACACTGA CCTCTGCGCC CCATGAGGAC ACGCTTAGCC TGCACATCCG GGCAGCAGGG 
CCCTGGACCA CTCGCCTCAG GGAGATCTAC TCAGCCCCGA CGGGTGACAG ATGTGCCAGA 
TACCCAAAGC TGTACCTTGA TGGACCATTT GGAGAGGGCC ACCAGGAGTG GCATAAGTTT 
GAGGTGTCAG TGTTAGTGGG AGGGGGCATT GGGGTCACCC CTTTTGCCTC CATCCTCAAA 
GACCTGGTCT TCAAGTCATC CGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACGC GGACCCAGCG TCAGTTTGAG TGGCTGGCTG ACATCATCCG AGAGGTGGAG 
GAGAATGACC ACCAGGACCT GGTGTCTGTG CACATCTACA TCACCCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACTAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTTCTGAAC 
CGGAGTCTAT TCACAGGCCT GCGCTCCATC ACCCACTTTG GCCGTCCCCC CTTTGAGCCC 
TTCTTCAACT CCCTGCAGGA GGTCCACCCC CAGGTCCGGA AGATCGGGGT GTTTAGCTGT 
GGCCCCCCTG GCATGACCAA GAATGTGGAA AAGGCCTGTC AGCTCATCAA CAGGCAGGAC 
CGGACTCACT TCTCCCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787954.1
CDS	155..4810
Translation	MGFCLALAWTLLVGAWTPLGAQNPISWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLGEPHLPNPRDLSNTISRGPAGLASLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIRIPPGDPMFDPDQRGDVVLPFQRSRWDPETGRSPSNPRDPANQVTGWLDGSAIYGSSHSWSDALRSFSRGQLASGPDPAFPRDSQNPLLMWAAPDPATGQNGPRGLYAFGAERGNREPFLQALGLLWFRYHNLWAQRLARQHPDWEDEELFQHARKRVIATYQNIAVYEWLPSFLQKTLPEYTGYRPFLDPSISSEFVAASEQFLSTMVPPGVYMRNASCHFQGVINRNSSVSRALRVCNSYWSREHPSLQSAEDVDALLLGMASQIAEREDHVLVEDVRDFWPGPLKFSRTDHLASCLQRGRDLGLPSYTKARAALGLSPITRWQDINPALSRSNDTVLEATAALYNQDLSWLELLPGGLLESHRDPGPLFSTIVLEQFVRLRDGDRYWFENTRNGLFSKKEIEEIRNTTLQDVLVAVINIDPSALQPNVFVWHKGDPCPQPRQLSTEGLPACAPSVVRDYFEGSGFGFGVTIGTLCCFPLVSLLSAWIVARLRMRNFKRLQGQDRQSIVSEKLVGGMEALEWQGHKEPCRPVLVYLQPGQIRVVDGRLTVLRTIQLQPPQKVNFVLSSNRGRRTLLLKIPKEYDLVLLFNLEEERQALVENLRGALKESGLSIQEWELREQELMRAAVTREQRRHLLETFFRHLFSQVLDINQADAGTLPLDSSQKVREALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHNSELRFTQLCVKGVEVPEVIKDLCRRASYISQDMICPSPRVSARCSRSDIETELTPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSCLHQTVQQFKRFIENYRRHIGCVAVFYAIAGGLFLERAYYYAFAAHHTGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRLIASTAIVLTVLHSVGHVVNVYLFSISPLSVLSCLFPGLFHDDGSELPQKYYWWFFQTVPGLTGVVLLLILAIMYVFASHHFRRRSFRGFWLTHHLYILLYVLLIIHGSFALIQLPRFHIFFLVPAIIYGGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSAPTGDRCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDHQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF

Target ORF information:

RefSeq Version	NM_175940.3
Organism	Homo sapiens(human)
Definition	Homo sapiens dual oxidase 1 (DUOX1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175940.3

ORF Insert Sequence:

ATGGGCTTCT GCCTGGCTCT AGCATGGACA CTTCTGGTTG GGGCATGGAC CCCTCTGGGA 
GCTCAGAACC CCATTTCGTG GGAGGTGCAG CGATTTGATG GGTGGTACAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCCCGG CTGCAGCGCC TGGTCCCAGC CAGCTATGCA 
GATGGCGTGT ACCAGCCCTT GGGAGAACCC CACCTGCCCA ACCCCCGAGA CCTTAGCAAC 
ACCATCTCAA GGGGCCCTGC AGGGCTGGCC TCCCTGAGAA ACCGCACAGT GTTGGGGGTC 
TTCTTTGGCT ATCACGTGCT TTCAGACCTG GTGAGCGTGG AAACTCCCGG CTGCCCCGCC 
GAGTTCCTCA ACATTCGCAT CCCGCCCGGA GACCCCATGT TCGACCCCGA CCAGCGCGGG 
GACGTGGTGC TGCCCTTCCA GAGAAGCCGC TGGGACCCCG AGACCGGACG GAGTCCCAGC 
AATCCCCGGG ACCCGGCCAA CCAGGTGACG GGCTGGCTGG ACGGCAGCGC CATCTATGGT 
TCCTCGCATT CCTGGAGCGA CGCGCTGCGG AGCTTCTCCA GGGGACAGCT GGCGTCGGGG 
CCCGACCCCG CTTTTCCCCG AGACTCGCAG AACCCCCTGC TCATGTGGGC GGCGCCCGAC 
CCCGCCACCG GGCAGAACGG GCCCCGGGGG CTGTACGCCT TCGGGGCAGA GAGAGGGAAC 
CGGGAACCCT TCCTGCAGGC GCTGGGCCTG CTCTGGTTCC GCTACCACAA CCTGTGGGCG 
CAGAGGCTGG CCCGCCAGCA CCCAGACTGG GAGGACGAGG AGCTGTTCCA GCACGCACGC 
AAGAGGGTCA TCGCCACCTA CCAGAACATC GCTGTGTATG AGTGGCTGCC CAGCTTCCTG 
CAGAAAACAC TCCCGGAGTA TACAGGATAC CGGCCATTTC TGGACCCCAG CATCTCCTCA 
GAGTTCGTGG CGGCCTCTGA GCAGTTCCTG TCCACCATGG TGCCCCCTGG CGTCTACATG 
AGAAATGCCA GCTGCCACTT CCAGGGGGTC ATCAATCGGA ACTCAAGTGT CTCCAGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGCCGT GAGCACCCAA GCCTACAAAG TGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATCGCAG AGCGAGAGGA CCATGTGTTG 
GTTGAAGATG TGCGGGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGCAC AGACCACCTG 
GCCAGCTGCC TGCAGCGGGG CCGGGATCTG GGCCTGCCCT CTTACACCAA GGCCAGGGCA 
GCACTGGGCT TGTCTCCCAT TACCCGCTGG CAGGACATCA ACCCTGCACT CTCCCGGAGC 
AATGACACTG TACTGGAGGC CACAGCTGCC CTGTACAACC AGGACTTATC CTGGCTAGAG 
CTGCTCCCTG GGGGACTCCT GGAGAGCCAC CGGGACCCTG GACCTCTGTT CAGCACCATC 
GTCCTTGAAC AATTTGTGCG GCTACGGGAT GGTGACCGCT ACTGGTTTGA GAACACCAGG 
AATGGGCTGT TCTCCAAGAA GGAGATTGAA GAAATCCGAA ATACCACCCT GCAGGACGTG 
CTGGTCGCTG TTATCAACAT TGACCCCAGT GCTCTGCAGC CCAATGTCTT TGTCTGGCAT 
AAAGGAGACC CCTGTCCGCA GCCGAGACAG CTCAGCACTG AAGGCCTGCC AGCGTGTGCT 
CCCTCTGTTG TTCGTGACTA TTTTGAGGGC AGTGGATTTG GCTTCGGGGT CACCATCGGG 
ACCCTCTGTT GCTTCCCTTT GGTGAGCCTG CTCAGTGCCT GGATTGTTGC CCGGCTCCGG 
ATGAGAAATT TCAAGAGGCT CCAGGGCCAG GACCGCCAGA GCATCGTGTC TGAGAAGCTC 
GTGGGAGGCA TGGAAGCTTT GGAATGGCAA GGCCACAAGG AGCCCTGCCG GCCCGTGCTT 
GTGTACCTGC AGCCCGGGCA GATCCGTGTG GTAGATGGCA GGCTCACCGT GCTCCGCACC 
ATCCAGCTGC AGCCTCCACA GAAGGTCAAC TTCGTCCTGT CCAGCAACCG TGGACGCCGC 
ACTCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTGC TGCTGTTTAA CTTGGAGGAA 
GAGCGGCAGG CGCTGGTGGA AAATCTCCGG GGAGCTCTGA AGGAGAGCGG GTTGAGCATC 
CAGGAGTGGG AGCTGCGGGA GCAGGAGCTG ATGAGAGCAG CTGTGACACG GGAGCAGCGG 
AGGCACCTCC TGGAGACCTT TTTCAGGCAC CTTTTCTCCC AGGTGCTGGA CATCAACCAG 
GCCGACGCAG GGACCCTGCC CCTGGACTCC TCCCAGAAGG TGCGGGAGGC CCTGACCTGT 
GAGCTGAGCA GGGCCGAGTT TGCCGAGTCC CTGGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TCTCTCTGGC TGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGAGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCTCCTGAGG AAAAGTCTCG CCTTATGTTC 
CGCATGTACG ACTTTGATGG GAATGGCCTC ATTTCCAAGG ATGAGTTCAT CAGGATGCTG 
AGATCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTG 
GAGTCCATGT TCCGGGAGTC GGGATTCCAG GACAAGGAGG AACTGACATG GGAAGATTTT 
CACTTCATGC TGCGGGACCA CAATAGCGAG CTCCGCTTCA CGCAGCTCTG TGTCAAAGGG 
GTGGAGGTGC CTGAAGTCAT CAAGGACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAT 
ATGATCTGTC CCTCTCCCAG AGTGAGTGCC CGCTGTTCCC GCAGCGACAT TGAGACTGAG 
TTGACACCTC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCCCAGGA GATTCGGCGG 
AGGTTTGGCA AGAAGGTAAC GTCATTCCAG CCCTTGCTGT TCACTGAGGC GCACCGAGAG 
AAGTTCCAAC GCAGCTGTCT CCACCAGACG GTGCAACAGT TCAAGCGCTT CATTGAGAAC 
TACCGGCGCC ACATCGGCTG CGTGGCCGTG TTCTACGCCA TCGCTGGGGG GCTTTTCCTG 
GAGAGGGCCT ACTACTACGC CTTTGCCGCA CATCACACGG GCATCACAGA CACCACCCGC 
GTGGGAATCA TCCTGTCGCG GGGCACAGCA GCCAGCATCT CTTTCATGTT CTCCTACATC 
TTGCTCACCA TGTGCCGCAA CCTCATCACC TTCCTGCGAG AAACCTTCCT CAACCGCTAC 
GTGCCCTTCG ACGCCGCCGT GGACTTCCAT CGCCTCATTG CCTCCACCGC CATCGTCCTC 
ACAGTCTTAC ACAGTGTGGG CCATGTGGTG AATGTGTACC TGTTCTCCAT CAGCCCCCTC 
AGCGTCCTCT CTTGCCTCTT TCCTGGCCTC TTCCATGATG ATGGGTCTGA GCTCCCCCAG 
AAGTATTACT GGTGGTTCTT CCAGACCGTA CCAGGCCTCA CGGGGGTTGT GCTGCTCCTG 
ATCCTGGCCA TCATGTATGT CTTTGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCACCACCT CTACATCCTG CTCTATGTCC TGCTCATCAT CCATGGTAGC 
TTTGCCCTGA TCCAGCTGCC CCGTTTCCAC ATCTTCTTCC TGGTCCCAGC AATCATCTAT 
GGGGGCGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCG 
GAGCTGCTGC CCTCAGGAGT GACCCACCTG CGGTTCCAGC GGCCCCAGGG CTTTGAGTAC 
AAGTCAGGGC AGTGGGTGCG GATCGCTTGC CTGGCTCTGG GGACCACCGA GTACCACCCC 
TTCACACTGA CCTCTGCGCC CCATGAGGAC ACGCTTAGCC TGCACATCCG GGCAGCAGGG 
CCCTGGACCA CTCGCCTCAG GGAGATCTAC TCAGCCCCGA CGGGTGACAG ATGTGCCAGA 
TACCCAAAGC TGTACCTTGA TGGACCATTT GGAGAGGGCC ACCAGGAGTG GCATAAGTTT 
GAGGTGTCAG TGTTAGTGGG AGGGGGCATT GGGGTCACCC CTTTTGCCTC CATCCTCAAA 
GACCTGGTCT TCAAGTCATC CGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACGC GGACCCAGCG TCAGTTTGAG TGGCTGGCTG ACATCATCCG AGAGGTGGAG 
GAGAATGACC ACCAGGACCT GGTGTCTGTG CACATCTACA TCACCCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACTAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTTCTGAAC 
CGGAGTCTAT TCACAGGCCT GCGCTCCATC ACCCACTTTG GCCGTCCCCC CTTTGAGCCC 
TTCTTCAACT CCCTGCAGGA GGTCCACCCC CAGGTCCGGA AGATCGGGGT GTTTAGCTGT 
GGCCCCCCTG GCATGACCAA GAATGTGGAA AAGGCCTGTC AGCTCATCAA CAGGCAGGAC 
CGGACTCACT TCTCCCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DUOX1 ( NM_017434.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_059130.2 Homo sapiens dual oxidase 1 (DUOX1), transcript variant 1, mRNA.

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CloneID	OHu10600
Clone ID Related Accession (Same CDS sequence)	NM_175940.2 , NM_175940.3 , NM_017434.4 , NM_017434.5
Accession Version	NM_017434.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	dual oxidase 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF213465.1, AF230495.1, AC051619.7 and CB852814.1. On Sep 11, 2013 this sequence version replaced NM_017434.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF230495.1, AK290372.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTTCT GCCTGGCTCT AGCATGGACA CTTCTGGTTG GGGCATGGAC CCCTCTGGGA 
GCTCAGAACC CCATTTCGTG GGAGGTGCAG CGATTTGATG GGTGGTACAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCCCGG CTGCAGCGCC TGGTCCCAGC CAGCTATGCA 
GATGGCGTGT ACCAGCCCTT GGGAGAACCC CACCTGCCCA ACCCCCGAGA CCTTAGCAAC 
ACCATCTCAA GGGGCCCTGC AGGGCTGGCC TCCCTGAGAA ACCGCACAGT GTTGGGGGTC 
TTCTTTGGCT ATCACGTGCT TTCAGACCTG GTGAGCGTGG AAACTCCCGG CTGCCCCGCC 
GAGTTCCTCA ACATTCGCAT CCCGCCCGGA GACCCCATGT TCGACCCCGA CCAGCGCGGG 
GACGTGGTGC TGCCCTTCCA GAGAAGCCGC TGGGACCCCG AGACCGGACG GAGTCCCAGC 
AATCCCCGGG ACCCGGCCAA CCAGGTGACG GGCTGGCTGG ACGGCAGCGC CATCTATGGT 
TCCTCGCATT CCTGGAGCGA CGCGCTGCGG AGCTTCTCCA GGGGACAGCT GGCGTCGGGG 
CCCGACCCCG CTTTTCCCCG AGACTCGCAG AACCCCCTGC TCATGTGGGC GGCGCCCGAC 
CCCGCCACCG GGCAGAACGG GCCCCGGGGG CTGTACGCCT TCGGGGCAGA GAGAGGGAAC 
CGGGAACCCT TCCTGCAGGC GCTGGGCCTG CTCTGGTTCC GCTACCACAA CCTGTGGGCG 
CAGAGGCTGG CCCGCCAGCA CCCAGACTGG GAGGACGAGG AGCTGTTCCA GCACGCACGC 
AAGAGGGTCA TCGCCACCTA CCAGAACATC GCTGTGTATG AGTGGCTGCC CAGCTTCCTG 
CAGAAAACAC TCCCGGAGTA TACAGGATAC CGGCCATTTC TGGACCCCAG CATCTCCTCA 
GAGTTCGTGG CGGCCTCTGA GCAGTTCCTG TCCACCATGG TGCCCCCTGG CGTCTACATG 
AGAAATGCCA GCTGCCACTT CCAGGGGGTC ATCAATCGGA ACTCAAGTGT CTCCAGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGCCGT GAGCACCCAA GCCTACAAAG TGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATCGCAG AGCGAGAGGA CCATGTGTTG 
GTTGAAGATG TGCGGGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGCAC AGACCACCTG 
GCCAGCTGCC TGCAGCGGGG CCGGGATCTG GGCCTGCCCT CTTACACCAA GGCCAGGGCA 
GCACTGGGCT TGTCTCCCAT TACCCGCTGG CAGGACATCA ACCCTGCACT CTCCCGGAGC 
AATGACACTG TACTGGAGGC CACAGCTGCC CTGTACAACC AGGACTTATC CTGGCTAGAG 
CTGCTCCCTG GGGGACTCCT GGAGAGCCAC CGGGACCCTG GACCTCTGTT CAGCACCATC 
GTCCTTGAAC AATTTGTGCG GCTACGGGAT GGTGACCGCT ACTGGTTTGA GAACACCAGG 
AATGGGCTGT TCTCCAAGAA GGAGATTGAA GAAATCCGAA ATACCACCCT GCAGGACGTG 
CTGGTCGCTG TTATCAACAT TGACCCCAGT GCTCTGCAGC CCAATGTCTT TGTCTGGCAT 
AAAGGAGACC CCTGTCCGCA GCCGAGACAG CTCAGCACTG AAGGCCTGCC AGCGTGTGCT 
CCCTCTGTTG TTCGTGACTA TTTTGAGGGC AGTGGATTTG GCTTCGGGGT CACCATCGGG 
ACCCTCTGTT GCTTCCCTTT GGTGAGCCTG CTCAGTGCCT GGATTGTTGC CCGGCTCCGG 
ATGAGAAATT TCAAGAGGCT CCAGGGCCAG GACCGCCAGA GCATCGTGTC TGAGAAGCTC 
GTGGGAGGCA TGGAAGCTTT GGAATGGCAA GGCCACAAGG AGCCCTGCCG GCCCGTGCTT 
GTGTACCTGC AGCCCGGGCA GATCCGTGTG GTAGATGGCA GGCTCACCGT GCTCCGCACC 
ATCCAGCTGC AGCCTCCACA GAAGGTCAAC TTCGTCCTGT CCAGCAACCG TGGACGCCGC 
ACTCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTGC TGCTGTTTAA CTTGGAGGAA 
GAGCGGCAGG CGCTGGTGGA AAATCTCCGG GGAGCTCTGA AGGAGAGCGG GTTGAGCATC 
CAGGAGTGGG AGCTGCGGGA GCAGGAGCTG ATGAGAGCAG CTGTGACACG GGAGCAGCGG 
AGGCACCTCC TGGAGACCTT TTTCAGGCAC CTTTTCTCCC AGGTGCTGGA CATCAACCAG 
GCCGACGCAG GGACCCTGCC CCTGGACTCC TCCCAGAAGG TGCGGGAGGC CCTGACCTGT 
GAGCTGAGCA GGGCCGAGTT TGCCGAGTCC CTGGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TCTCTCTGGC TGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGAGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCTCCTGAGG AAAAGTCTCG CCTTATGTTC 
CGCATGTACG ACTTTGATGG GAATGGCCTC ATTTCCAAGG ATGAGTTCAT CAGGATGCTG 
AGATCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTG 
GAGTCCATGT TCCGGGAGTC GGGATTCCAG GACAAGGAGG AACTGACATG GGAAGATTTT 
CACTTCATGC TGCGGGACCA CAATAGCGAG CTCCGCTTCA CGCAGCTCTG TGTCAAAGGG 
GTGGAGGTGC CTGAAGTCAT CAAGGACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAT 
ATGATCTGTC CCTCTCCCAG AGTGAGTGCC CGCTGTTCCC GCAGCGACAT TGAGACTGAG 
TTGACACCTC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCCCAGGA GATTCGGCGG 
AGGTTTGGCA AGAAGGTAAC GTCATTCCAG CCCTTGCTGT TCACTGAGGC GCACCGAGAG 
AAGTTCCAAC GCAGCTGTCT CCACCAGACG GTGCAACAGT TCAAGCGCTT CATTGAGAAC 
TACCGGCGCC ACATCGGCTG CGTGGCCGTG TTCTACGCCA TCGCTGGGGG GCTTTTCCTG 
GAGAGGGCCT ACTACTACGC CTTTGCCGCA CATCACACGG GCATCACAGA CACCACCCGC 
GTGGGAATCA TCCTGTCGCG GGGCACAGCA GCCAGCATCT CTTTCATGTT CTCCTACATC 
TTGCTCACCA TGTGCCGCAA CCTCATCACC TTCCTGCGAG AAACCTTCCT CAACCGCTAC 
GTGCCCTTCG ACGCCGCCGT GGACTTCCAT CGCCTCATTG CCTCCACCGC CATCGTCCTC 
ACAGTCTTAC ACAGTGTGGG CCATGTGGTG AATGTGTACC TGTTCTCCAT CAGCCCCCTC 
AGCGTCCTCT CTTGCCTCTT TCCTGGCCTC TTCCATGATG ATGGGTCTGA GCTCCCCCAG 
AAGTATTACT GGTGGTTCTT CCAGACCGTA CCAGGCCTCA CGGGGGTTGT GCTGCTCCTG 
ATCCTGGCCA TCATGTATGT CTTTGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCACCACCT CTACATCCTG CTCTATGTCC TGCTCATCAT CCATGGTAGC 
TTTGCCCTGA TCCAGCTGCC CCGTTTCCAC ATCTTCTTCC TGGTCCCAGC AATCATCTAT 
GGGGGCGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCG 
GAGCTGCTGC CCTCAGGAGT GACCCACCTG CGGTTCCAGC GGCCCCAGGG CTTTGAGTAC 
AAGTCAGGGC AGTGGGTGCG GATCGCTTGC CTGGCTCTGG GGACCACCGA GTACCACCCC 
TTCACACTGA CCTCTGCGCC CCATGAGGAC ACGCTTAGCC TGCACATCCG GGCAGCAGGG 
CCCTGGACCA CTCGCCTCAG GGAGATCTAC TCAGCCCCGA CGGGTGACAG ATGTGCCAGA 
TACCCAAAGC TGTACCTTGA TGGACCATTT GGAGAGGGCC ACCAGGAGTG GCATAAGTTT 
GAGGTGTCAG TGTTAGTGGG AGGGGGCATT GGGGTCACCC CTTTTGCCTC CATCCTCAAA 
GACCTGGTCT TCAAGTCATC CGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACGC GGACCCAGCG TCAGTTTGAG TGGCTGGCTG ACATCATCCG AGAGGTGGAG 
GAGAATGACC ACCAGGACCT GGTGTCTGTG CACATCTACA TCACCCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACTAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTTCTGAAC 
CGGAGTCTAT TCACAGGCCT GCGCTCCATC ACCCACTTTG GCCGTCCCCC CTTTGAGCCC 
TTCTTCAACT CCCTGCAGGA GGTCCACCCC CAGGTCCGGA AGATCGGGGT GTTTAGCTGT 
GGCCCCCCTG GCATGACCAA GAATGTGGAA AAGGCCTGTC AGCTCATCAA CAGGCAGGAC 
CGGACTCACT TCTCCCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059130.2
CDS	347..5002
Translation	MGFCLALAWTLLVGAWTPLGAQNPISWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLGEPHLPNPRDLSNTISRGPAGLASLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIRIPPGDPMFDPDQRGDVVLPFQRSRWDPETGRSPSNPRDPANQVTGWLDGSAIYGSSHSWSDALRSFSRGQLASGPDPAFPRDSQNPLLMWAAPDPATGQNGPRGLYAFGAERGNREPFLQALGLLWFRYHNLWAQRLARQHPDWEDEELFQHARKRVIATYQNIAVYEWLPSFLQKTLPEYTGYRPFLDPSISSEFVAASEQFLSTMVPPGVYMRNASCHFQGVINRNSSVSRALRVCNSYWSREHPSLQSAEDVDALLLGMASQIAEREDHVLVEDVRDFWPGPLKFSRTDHLASCLQRGRDLGLPSYTKARAALGLSPITRWQDINPALSRSNDTVLEATAALYNQDLSWLELLPGGLLESHRDPGPLFSTIVLEQFVRLRDGDRYWFENTRNGLFSKKEIEEIRNTTLQDVLVAVINIDPSALQPNVFVWHKGDPCPQPRQLSTEGLPACAPSVVRDYFEGSGFGFGVTIGTLCCFPLVSLLSAWIVARLRMRNFKRLQGQDRQSIVSEKLVGGMEALEWQGHKEPCRPVLVYLQPGQIRVVDGRLTVLRTIQLQPPQKVNFVLSSNRGRRTLLLKIPKEYDLVLLFNLEEERQALVENLRGALKESGLSIQEWELREQELMRAAVTREQRRHLLETFFRHLFSQVLDINQADAGTLPLDSSQKVREALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHNSELRFTQLCVKGVEVPEVIKDLCRRASYISQDMICPSPRVSARCSRSDIETELTPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSCLHQTVQQFKRFIENYRRHIGCVAVFYAIAGGLFLERAYYYAFAAHHTGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRLIASTAIVLTVLHSVGHVVNVYLFSISPLSVLSCLFPGLFHDDGSELPQKYYWWFFQTVPGLTGVVLLLILAIMYVFASHHFRRRSFRGFWLTHHLYILLYVLLIIHGSFALIQLPRFHIFFLVPAIIYGGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSAPTGDRCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDHQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF

Target ORF information:

RefSeq Version	NM_017434.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dual oxidase 1 (DUOX1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017434.4

ORF Insert Sequence:

ATGGGCTTCT GCCTGGCTCT AGCATGGACA CTTCTGGTTG GGGCATGGAC CCCTCTGGGA 
GCTCAGAACC CCATTTCGTG GGAGGTGCAG CGATTTGATG GGTGGTACAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCCCGG CTGCAGCGCC TGGTCCCAGC CAGCTATGCA 
GATGGCGTGT ACCAGCCCTT GGGAGAACCC CACCTGCCCA ACCCCCGAGA CCTTAGCAAC 
ACCATCTCAA GGGGCCCTGC AGGGCTGGCC TCCCTGAGAA ACCGCACAGT GTTGGGGGTC 
TTCTTTGGCT ATCACGTGCT TTCAGACCTG GTGAGCGTGG AAACTCCCGG CTGCCCCGCC 
GAGTTCCTCA ACATTCGCAT CCCGCCCGGA GACCCCATGT TCGACCCCGA CCAGCGCGGG 
GACGTGGTGC TGCCCTTCCA GAGAAGCCGC TGGGACCCCG AGACCGGACG GAGTCCCAGC 
AATCCCCGGG ACCCGGCCAA CCAGGTGACG GGCTGGCTGG ACGGCAGCGC CATCTATGGT 
TCCTCGCATT CCTGGAGCGA CGCGCTGCGG AGCTTCTCCA GGGGACAGCT GGCGTCGGGG 
CCCGACCCCG CTTTTCCCCG AGACTCGCAG AACCCCCTGC TCATGTGGGC GGCGCCCGAC 
CCCGCCACCG GGCAGAACGG GCCCCGGGGG CTGTACGCCT TCGGGGCAGA GAGAGGGAAC 
CGGGAACCCT TCCTGCAGGC GCTGGGCCTG CTCTGGTTCC GCTACCACAA CCTGTGGGCG 
CAGAGGCTGG CCCGCCAGCA CCCAGACTGG GAGGACGAGG AGCTGTTCCA GCACGCACGC 
AAGAGGGTCA TCGCCACCTA CCAGAACATC GCTGTGTATG AGTGGCTGCC CAGCTTCCTG 
CAGAAAACAC TCCCGGAGTA TACAGGATAC CGGCCATTTC TGGACCCCAG CATCTCCTCA 
GAGTTCGTGG CGGCCTCTGA GCAGTTCCTG TCCACCATGG TGCCCCCTGG CGTCTACATG 
AGAAATGCCA GCTGCCACTT CCAGGGGGTC ATCAATCGGA ACTCAAGTGT CTCCAGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGCCGT GAGCACCCAA GCCTACAAAG TGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATCGCAG AGCGAGAGGA CCATGTGTTG 
GTTGAAGATG TGCGGGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGCAC AGACCACCTG 
GCCAGCTGCC TGCAGCGGGG CCGGGATCTG GGCCTGCCCT CTTACACCAA GGCCAGGGCA 
GCACTGGGCT TGTCTCCCAT TACCCGCTGG CAGGACATCA ACCCTGCACT CTCCCGGAGC 
AATGACACTG TACTGGAGGC CACAGCTGCC CTGTACAACC AGGACTTATC CTGGCTAGAG 
CTGCTCCCTG GGGGACTCCT GGAGAGCCAC CGGGACCCTG GACCTCTGTT CAGCACCATC 
GTCCTTGAAC AATTTGTGCG GCTACGGGAT GGTGACCGCT ACTGGTTTGA GAACACCAGG 
AATGGGCTGT TCTCCAAGAA GGAGATTGAA GAAATCCGAA ATACCACCCT GCAGGACGTG 
CTGGTCGCTG TTATCAACAT TGACCCCAGT GCTCTGCAGC CCAATGTCTT TGTCTGGCAT 
AAAGGAGACC CCTGTCCGCA GCCGAGACAG CTCAGCACTG AAGGCCTGCC AGCGTGTGCT 
CCCTCTGTTG TTCGTGACTA TTTTGAGGGC AGTGGATTTG GCTTCGGGGT CACCATCGGG 
ACCCTCTGTT GCTTCCCTTT GGTGAGCCTG CTCAGTGCCT GGATTGTTGC CCGGCTCCGG 
ATGAGAAATT TCAAGAGGCT CCAGGGCCAG GACCGCCAGA GCATCGTGTC TGAGAAGCTC 
GTGGGAGGCA TGGAAGCTTT GGAATGGCAA GGCCACAAGG AGCCCTGCCG GCCCGTGCTT 
GTGTACCTGC AGCCCGGGCA GATCCGTGTG GTAGATGGCA GGCTCACCGT GCTCCGCACC 
ATCCAGCTGC AGCCTCCACA GAAGGTCAAC TTCGTCCTGT CCAGCAACCG TGGACGCCGC 
ACTCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTGC TGCTGTTTAA CTTGGAGGAA 
GAGCGGCAGG CGCTGGTGGA AAATCTCCGG GGAGCTCTGA AGGAGAGCGG GTTGAGCATC 
CAGGAGTGGG AGCTGCGGGA GCAGGAGCTG ATGAGAGCAG CTGTGACACG GGAGCAGCGG 
AGGCACCTCC TGGAGACCTT TTTCAGGCAC CTTTTCTCCC AGGTGCTGGA CATCAACCAG 
GCCGACGCAG GGACCCTGCC CCTGGACTCC TCCCAGAAGG TGCGGGAGGC CCTGACCTGT 
GAGCTGAGCA GGGCCGAGTT TGCCGAGTCC CTGGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TCTCTCTGGC TGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGAGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCTCCTGAGG AAAAGTCTCG CCTTATGTTC 
CGCATGTACG ACTTTGATGG GAATGGCCTC ATTTCCAAGG ATGAGTTCAT CAGGATGCTG 
AGATCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTG 
GAGTCCATGT TCCGGGAGTC GGGATTCCAG GACAAGGAGG AACTGACATG GGAAGATTTT 
CACTTCATGC TGCGGGACCA CAATAGCGAG CTCCGCTTCA CGCAGCTCTG TGTCAAAGGG 
GTGGAGGTGC CTGAAGTCAT CAAGGACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAT 
ATGATCTGTC CCTCTCCCAG AGTGAGTGCC CGCTGTTCCC GCAGCGACAT TGAGACTGAG 
TTGACACCTC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCCCAGGA GATTCGGCGG 
AGGTTTGGCA AGAAGGTAAC GTCATTCCAG CCCTTGCTGT TCACTGAGGC GCACCGAGAG 
AAGTTCCAAC GCAGCTGTCT CCACCAGACG GTGCAACAGT TCAAGCGCTT CATTGAGAAC 
TACCGGCGCC ACATCGGCTG CGTGGCCGTG TTCTACGCCA TCGCTGGGGG GCTTTTCCTG 
GAGAGGGCCT ACTACTACGC CTTTGCCGCA CATCACACGG GCATCACAGA CACCACCCGC 
GTGGGAATCA TCCTGTCGCG GGGCACAGCA GCCAGCATCT CTTTCATGTT CTCCTACATC 
TTGCTCACCA TGTGCCGCAA CCTCATCACC TTCCTGCGAG AAACCTTCCT CAACCGCTAC 
GTGCCCTTCG ACGCCGCCGT GGACTTCCAT CGCCTCATTG CCTCCACCGC CATCGTCCTC 
ACAGTCTTAC ACAGTGTGGG CCATGTGGTG AATGTGTACC TGTTCTCCAT CAGCCCCCTC 
AGCGTCCTCT CTTGCCTCTT TCCTGGCCTC TTCCATGATG ATGGGTCTGA GCTCCCCCAG 
AAGTATTACT GGTGGTTCTT CCAGACCGTA CCAGGCCTCA CGGGGGTTGT GCTGCTCCTG 
ATCCTGGCCA TCATGTATGT CTTTGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCACCACCT CTACATCCTG CTCTATGTCC TGCTCATCAT CCATGGTAGC 
TTTGCCCTGA TCCAGCTGCC CCGTTTCCAC ATCTTCTTCC TGGTCCCAGC AATCATCTAT 
GGGGGCGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCG 
GAGCTGCTGC CCTCAGGAGT GACCCACCTG CGGTTCCAGC GGCCCCAGGG CTTTGAGTAC 
AAGTCAGGGC AGTGGGTGCG GATCGCTTGC CTGGCTCTGG GGACCACCGA GTACCACCCC 
TTCACACTGA CCTCTGCGCC CCATGAGGAC ACGCTTAGCC TGCACATCCG GGCAGCAGGG 
CCCTGGACCA CTCGCCTCAG GGAGATCTAC TCAGCCCCGA CGGGTGACAG ATGTGCCAGA 
TACCCAAAGC TGTACCTTGA TGGACCATTT GGAGAGGGCC ACCAGGAGTG GCATAAGTTT 
GAGGTGTCAG TGTTAGTGGG AGGGGGCATT GGGGTCACCC CTTTTGCCTC CATCCTCAAA 
GACCTGGTCT TCAAGTCATC CGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACGC GGACCCAGCG TCAGTTTGAG TGGCTGGCTG ACATCATCCG AGAGGTGGAG 
GAGAATGACC ACCAGGACCT GGTGTCTGTG CACATCTACA TCACCCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACTAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTTCTGAAC 
CGGAGTCTAT TCACAGGCCT GCGCTCCATC ACCCACTTTG GCCGTCCCCC CTTTGAGCCC 
TTCTTCAACT CCCTGCAGGA GGTCCACCCC CAGGTCCGGA AGATCGGGGT GTTTAGCTGT 
GGCCCCCCTG GCATGACCAA GAATGTGGAA AAGGCCTGTC AGCTCATCAA CAGGCAGGAC 
CGGACTCACT TCTCCCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DUOX1 ( NM_017434.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_059130.2 Homo sapiens dual oxidase 1 (DUOX1), transcript variant 1, mRNA.

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CloneID	OHu10600
Clone ID Related Accession (Same CDS sequence)	NM_175940.2 , NM_175940.3 , NM_017434.4 , NM_017434.5
Accession Version	NM_017434.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	dual oxidase 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF213465.1, AF230495.1, AC051619.7 and CB852814.1. On May 31, 2019 this sequence version replaced NM_017434.4. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF230495.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTTCT GCCTGGCTCT AGCATGGACA CTTCTGGTTG GGGCATGGAC CCCTCTGGGA 
GCTCAGAACC CCATTTCGTG GGAGGTGCAG CGATTTGATG GGTGGTACAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCCCGG CTGCAGCGCC TGGTCCCAGC CAGCTATGCA 
GATGGCGTGT ACCAGCCCTT GGGAGAACCC CACCTGCCCA ACCCCCGAGA CCTTAGCAAC 
ACCATCTCAA GGGGCCCTGC AGGGCTGGCC TCCCTGAGAA ACCGCACAGT GTTGGGGGTC 
TTCTTTGGCT ATCACGTGCT TTCAGACCTG GTGAGCGTGG AAACTCCCGG CTGCCCCGCC 
GAGTTCCTCA ACATTCGCAT CCCGCCCGGA GACCCCATGT TCGACCCCGA CCAGCGCGGG 
GACGTGGTGC TGCCCTTCCA GAGAAGCCGC TGGGACCCCG AGACCGGACG GAGTCCCAGC 
AATCCCCGGG ACCCGGCCAA CCAGGTGACG GGCTGGCTGG ACGGCAGCGC CATCTATGGT 
TCCTCGCATT CCTGGAGCGA CGCGCTGCGG AGCTTCTCCA GGGGACAGCT GGCGTCGGGG 
CCCGACCCCG CTTTTCCCCG AGACTCGCAG AACCCCCTGC TCATGTGGGC GGCGCCCGAC 
CCCGCCACCG GGCAGAACGG GCCCCGGGGG CTGTACGCCT TCGGGGCAGA GAGAGGGAAC 
CGGGAACCCT TCCTGCAGGC GCTGGGCCTG CTCTGGTTCC GCTACCACAA CCTGTGGGCG 
CAGAGGCTGG CCCGCCAGCA CCCAGACTGG GAGGACGAGG AGCTGTTCCA GCACGCACGC 
AAGAGGGTCA TCGCCACCTA CCAGAACATC GCTGTGTATG AGTGGCTGCC CAGCTTCCTG 
CAGAAAACAC TCCCGGAGTA TACAGGATAC CGGCCATTTC TGGACCCCAG CATCTCCTCA 
GAGTTCGTGG CGGCCTCTGA GCAGTTCCTG TCCACCATGG TGCCCCCTGG CGTCTACATG 
AGAAATGCCA GCTGCCACTT CCAGGGGGTC ATCAATCGGA ACTCAAGTGT CTCCAGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGCCGT GAGCACCCAA GCCTACAAAG TGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATCGCAG AGCGAGAGGA CCATGTGTTG 
GTTGAAGATG TGCGGGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGCAC AGACCACCTG 
GCCAGCTGCC TGCAGCGGGG CCGGGATCTG GGCCTGCCCT CTTACACCAA GGCCAGGGCA 
GCACTGGGCT TGTCTCCCAT TACCCGCTGG CAGGACATCA ACCCTGCACT CTCCCGGAGC 
AATGACACTG TACTGGAGGC CACAGCTGCC CTGTACAACC AGGACTTATC CTGGCTAGAG 
CTGCTCCCTG GGGGACTCCT GGAGAGCCAC CGGGACCCTG GACCTCTGTT CAGCACCATC 
GTCCTTGAAC AATTTGTGCG GCTACGGGAT GGTGACCGCT ACTGGTTTGA GAACACCAGG 
AATGGGCTGT TCTCCAAGAA GGAGATTGAA GAAATCCGAA ATACCACCCT GCAGGACGTG 
CTGGTCGCTG TTATCAACAT TGACCCCAGT GCTCTGCAGC CCAATGTCTT TGTCTGGCAT 
AAAGGAGACC CCTGTCCGCA GCCGAGACAG CTCAGCACTG AAGGCCTGCC AGCGTGTGCT 
CCCTCTGTTG TTCGTGACTA TTTTGAGGGC AGTGGATTTG GCTTCGGGGT CACCATCGGG 
ACCCTCTGTT GCTTCCCTTT GGTGAGCCTG CTCAGTGCCT GGATTGTTGC CCGGCTCCGG 
ATGAGAAATT TCAAGAGGCT CCAGGGCCAG GACCGCCAGA GCATCGTGTC TGAGAAGCTC 
GTGGGAGGCA TGGAAGCTTT GGAATGGCAA GGCCACAAGG AGCCCTGCCG GCCCGTGCTT 
GTGTACCTGC AGCCCGGGCA GATCCGTGTG GTAGATGGCA GGCTCACCGT GCTCCGCACC 
ATCCAGCTGC AGCCTCCACA GAAGGTCAAC TTCGTCCTGT CCAGCAACCG TGGACGCCGC 
ACTCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTGC TGCTGTTTAA CTTGGAGGAA 
GAGCGGCAGG CGCTGGTGGA AAATCTCCGG GGAGCTCTGA AGGAGAGCGG GTTGAGCATC 
CAGGAGTGGG AGCTGCGGGA GCAGGAGCTG ATGAGAGCAG CTGTGACACG GGAGCAGCGG 
AGGCACCTCC TGGAGACCTT TTTCAGGCAC CTTTTCTCCC AGGTGCTGGA CATCAACCAG 
GCCGACGCAG GGACCCTGCC CCTGGACTCC TCCCAGAAGG TGCGGGAGGC CCTGACCTGT 
GAGCTGAGCA GGGCCGAGTT TGCCGAGTCC CTGGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TCTCTCTGGC TGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGAGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCTCCTGAGG AAAAGTCTCG CCTTATGTTC 
CGCATGTACG ACTTTGATGG GAATGGCCTC ATTTCCAAGG ATGAGTTCAT CAGGATGCTG 
AGATCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTG 
GAGTCCATGT TCCGGGAGTC GGGATTCCAG GACAAGGAGG AACTGACATG GGAAGATTTT 
CACTTCATGC TGCGGGACCA CAATAGCGAG CTCCGCTTCA CGCAGCTCTG TGTCAAAGGG 
GTGGAGGTGC CTGAAGTCAT CAAGGACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAT 
ATGATCTGTC CCTCTCCCAG AGTGAGTGCC CGCTGTTCCC GCAGCGACAT TGAGACTGAG 
TTGACACCTC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCCCAGGA GATTCGGCGG 
AGGTTTGGCA AGAAGGTAAC GTCATTCCAG CCCTTGCTGT TCACTGAGGC GCACCGAGAG 
AAGTTCCAAC GCAGCTGTCT CCACCAGACG GTGCAACAGT TCAAGCGCTT CATTGAGAAC 
TACCGGCGCC ACATCGGCTG CGTGGCCGTG TTCTACGCCA TCGCTGGGGG GCTTTTCCTG 
GAGAGGGCCT ACTACTACGC CTTTGCCGCA CATCACACGG GCATCACAGA CACCACCCGC 
GTGGGAATCA TCCTGTCGCG GGGCACAGCA GCCAGCATCT CTTTCATGTT CTCCTACATC 
TTGCTCACCA TGTGCCGCAA CCTCATCACC TTCCTGCGAG AAACCTTCCT CAACCGCTAC 
GTGCCCTTCG ACGCCGCCGT GGACTTCCAT CGCCTCATTG CCTCCACCGC CATCGTCCTC 
ACAGTCTTAC ACAGTGTGGG CCATGTGGTG AATGTGTACC TGTTCTCCAT CAGCCCCCTC 
AGCGTCCTCT CTTGCCTCTT TCCTGGCCTC TTCCATGATG ATGGGTCTGA GCTCCCCCAG 
AAGTATTACT GGTGGTTCTT CCAGACCGTA CCAGGCCTCA CGGGGGTTGT GCTGCTCCTG 
ATCCTGGCCA TCATGTATGT CTTTGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCACCACCT CTACATCCTG CTCTATGTCC TGCTCATCAT CCATGGTAGC 
TTTGCCCTGA TCCAGCTGCC CCGTTTCCAC ATCTTCTTCC TGGTCCCAGC AATCATCTAT 
GGGGGCGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCG 
GAGCTGCTGC CCTCAGGAGT GACCCACCTG CGGTTCCAGC GGCCCCAGGG CTTTGAGTAC 
AAGTCAGGGC AGTGGGTGCG GATCGCTTGC CTGGCTCTGG GGACCACCGA GTACCACCCC 
TTCACACTGA CCTCTGCGCC CCATGAGGAC ACGCTTAGCC TGCACATCCG GGCAGCAGGG 
CCCTGGACCA CTCGCCTCAG GGAGATCTAC TCAGCCCCGA CGGGTGACAG ATGTGCCAGA 
TACCCAAAGC TGTACCTTGA TGGACCATTT GGAGAGGGCC ACCAGGAGTG GCATAAGTTT 
GAGGTGTCAG TGTTAGTGGG AGGGGGCATT GGGGTCACCC CTTTTGCCTC CATCCTCAAA 
GACCTGGTCT TCAAGTCATC CGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACGC GGACCCAGCG TCAGTTTGAG TGGCTGGCTG ACATCATCCG AGAGGTGGAG 
GAGAATGACC ACCAGGACCT GGTGTCTGTG CACATCTACA TCACCCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACTAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTTCTGAAC 
CGGAGTCTAT TCACAGGCCT GCGCTCCATC ACCCACTTTG GCCGTCCCCC CTTTGAGCCC 
TTCTTCAACT CCCTGCAGGA GGTCCACCCC CAGGTCCGGA AGATCGGGGT GTTTAGCTGT 
GGCCCCCCTG GCATGACCAA GAATGTGGAA AAGGCCTGTC AGCTCATCAA CAGGCAGGAC 
CGGACTCACT TCTCCCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059130.2
CDS	347..5002
Translation	MGFCLALAWTLLVGAWTPLGAQNPISWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLGEPHLPNPRDLSNTISRGPAGLASLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIRIPPGDPMFDPDQRGDVVLPFQRSRWDPETGRSPSNPRDPANQVTGWLDGSAIYGSSHSWSDALRSFSRGQLASGPDPAFPRDSQNPLLMWAAPDPATGQNGPRGLYAFGAERGNREPFLQALGLLWFRYHNLWAQRLARQHPDWEDEELFQHARKRVIATYQNIAVYEWLPSFLQKTLPEYTGYRPFLDPSISSEFVAASEQFLSTMVPPGVYMRNASCHFQGVINRNSSVSRALRVCNSYWSREHPSLQSAEDVDALLLGMASQIAEREDHVLVEDVRDFWPGPLKFSRTDHLASCLQRGRDLGLPSYTKARAALGLSPITRWQDINPALSRSNDTVLEATAALYNQDLSWLELLPGGLLESHRDPGPLFSTIVLEQFVRLRDGDRYWFENTRNGLFSKKEIEEIRNTTLQDVLVAVINIDPSALQPNVFVWHKGDPCPQPRQLSTEGLPACAPSVVRDYFEGSGFGFGVTIGTLCCFPLVSLLSAWIVARLRMRNFKRLQGQDRQSIVSEKLVGGMEALEWQGHKEPCRPVLVYLQPGQIRVVDGRLTVLRTIQLQPPQKVNFVLSSNRGRRTLLLKIPKEYDLVLLFNLEEERQALVENLRGALKESGLSIQEWELREQELMRAAVTREQRRHLLETFFRHLFSQVLDINQADAGTLPLDSSQKVREALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHNSELRFTQLCVKGVEVPEVIKDLCRRASYISQDMICPSPRVSARCSRSDIETELTPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSCLHQTVQQFKRFIENYRRHIGCVAVFYAIAGGLFLERAYYYAFAAHHTGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRLIASTAIVLTVLHSVGHVVNVYLFSISPLSVLSCLFPGLFHDDGSELPQKYYWWFFQTVPGLTGVVLLLILAIMYVFASHHFRRRSFRGFWLTHHLYILLYVLLIIHGSFALIQLPRFHIFFLVPAIIYGGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSAPTGDRCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDHQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF

Target ORF information:

RefSeq Version	NM_017434.5
Organism	Homo sapiens(human)
Definition	Homo sapiens dual oxidase 1 (DUOX1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017434.5

ORF Insert Sequence:

ATGGGCTTCT GCCTGGCTCT AGCATGGACA CTTCTGGTTG GGGCATGGAC CCCTCTGGGA 
GCTCAGAACC CCATTTCGTG GGAGGTGCAG CGATTTGATG GGTGGTACAA CAACCTCATG 
GAGCACAGAT GGGGCAGCAA AGGCTCCCGG CTGCAGCGCC TGGTCCCAGC CAGCTATGCA 
GATGGCGTGT ACCAGCCCTT GGGAGAACCC CACCTGCCCA ACCCCCGAGA CCTTAGCAAC 
ACCATCTCAA GGGGCCCTGC AGGGCTGGCC TCCCTGAGAA ACCGCACAGT GTTGGGGGTC 
TTCTTTGGCT ATCACGTGCT TTCAGACCTG GTGAGCGTGG AAACTCCCGG CTGCCCCGCC 
GAGTTCCTCA ACATTCGCAT CCCGCCCGGA GACCCCATGT TCGACCCCGA CCAGCGCGGG 
GACGTGGTGC TGCCCTTCCA GAGAAGCCGC TGGGACCCCG AGACCGGACG GAGTCCCAGC 
AATCCCCGGG ACCCGGCCAA CCAGGTGACG GGCTGGCTGG ACGGCAGCGC CATCTATGGT 
TCCTCGCATT CCTGGAGCGA CGCGCTGCGG AGCTTCTCCA GGGGACAGCT GGCGTCGGGG 
CCCGACCCCG CTTTTCCCCG AGACTCGCAG AACCCCCTGC TCATGTGGGC GGCGCCCGAC 
CCCGCCACCG GGCAGAACGG GCCCCGGGGG CTGTACGCCT TCGGGGCAGA GAGAGGGAAC 
CGGGAACCCT TCCTGCAGGC GCTGGGCCTG CTCTGGTTCC GCTACCACAA CCTGTGGGCG 
CAGAGGCTGG CCCGCCAGCA CCCAGACTGG GAGGACGAGG AGCTGTTCCA GCACGCACGC 
AAGAGGGTCA TCGCCACCTA CCAGAACATC GCTGTGTATG AGTGGCTGCC CAGCTTCCTG 
CAGAAAACAC TCCCGGAGTA TACAGGATAC CGGCCATTTC TGGACCCCAG CATCTCCTCA 
GAGTTCGTGG CGGCCTCTGA GCAGTTCCTG TCCACCATGG TGCCCCCTGG CGTCTACATG 
AGAAATGCCA GCTGCCACTT CCAGGGGGTC ATCAATCGGA ACTCAAGTGT CTCCAGAGCT 
CTCCGGGTCT GCAACAGCTA CTGGAGCCGT GAGCACCCAA GCCTACAAAG TGCTGAAGAT 
GTGGATGCAC TGCTGCTGGG CATGGCCTCC CAGATCGCAG AGCGAGAGGA CCATGTGTTG 
GTTGAAGATG TGCGGGATTT CTGGCCTGGG CCACTGAAGT TTTCCCGCAC AGACCACCTG 
GCCAGCTGCC TGCAGCGGGG CCGGGATCTG GGCCTGCCCT CTTACACCAA GGCCAGGGCA 
GCACTGGGCT TGTCTCCCAT TACCCGCTGG CAGGACATCA ACCCTGCACT CTCCCGGAGC 
AATGACACTG TACTGGAGGC CACAGCTGCC CTGTACAACC AGGACTTATC CTGGCTAGAG 
CTGCTCCCTG GGGGACTCCT GGAGAGCCAC CGGGACCCTG GACCTCTGTT CAGCACCATC 
GTCCTTGAAC AATTTGTGCG GCTACGGGAT GGTGACCGCT ACTGGTTTGA GAACACCAGG 
AATGGGCTGT TCTCCAAGAA GGAGATTGAA GAAATCCGAA ATACCACCCT GCAGGACGTG 
CTGGTCGCTG TTATCAACAT TGACCCCAGT GCTCTGCAGC CCAATGTCTT TGTCTGGCAT 
AAAGGAGACC CCTGTCCGCA GCCGAGACAG CTCAGCACTG AAGGCCTGCC AGCGTGTGCT 
CCCTCTGTTG TTCGTGACTA TTTTGAGGGC AGTGGATTTG GCTTCGGGGT CACCATCGGG 
ACCCTCTGTT GCTTCCCTTT GGTGAGCCTG CTCAGTGCCT GGATTGTTGC CCGGCTCCGG 
ATGAGAAATT TCAAGAGGCT CCAGGGCCAG GACCGCCAGA GCATCGTGTC TGAGAAGCTC 
GTGGGAGGCA TGGAAGCTTT GGAATGGCAA GGCCACAAGG AGCCCTGCCG GCCCGTGCTT 
GTGTACCTGC AGCCCGGGCA GATCCGTGTG GTAGATGGCA GGCTCACCGT GCTCCGCACC 
ATCCAGCTGC AGCCTCCACA GAAGGTCAAC TTCGTCCTGT CCAGCAACCG TGGACGCCGC 
ACTCTGCTGC TCAAGATCCC CAAGGAGTAT GACCTGGTGC TGCTGTTTAA CTTGGAGGAA 
GAGCGGCAGG CGCTGGTGGA AAATCTCCGG GGAGCTCTGA AGGAGAGCGG GTTGAGCATC 
CAGGAGTGGG AGCTGCGGGA GCAGGAGCTG ATGAGAGCAG CTGTGACACG GGAGCAGCGG 
AGGCACCTCC TGGAGACCTT TTTCAGGCAC CTTTTCTCCC AGGTGCTGGA CATCAACCAG 
GCCGACGCAG GGACCCTGCC CCTGGACTCC TCCCAGAAGG TGCGGGAGGC CCTGACCTGT 
GAGCTGAGCA GGGCCGAGTT TGCCGAGTCC CTGGGCCTCA AGCCCCAGGA CATGTTTGTG 
GAGTCCATGT TCTCTCTGGC TGACAAGGAT GGCAATGGCT ACCTGTCCTT CCGAGAGTTC 
CTGGACATCC TGGTGGTCTT CATGAAAGGC TCTCCTGAGG AAAAGTCTCG CCTTATGTTC 
CGCATGTACG ACTTTGATGG GAATGGCCTC ATTTCCAAGG ATGAGTTCAT CAGGATGCTG 
AGATCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG CCCAGCTGGC TGAGGTGGTG 
GAGTCCATGT TCCGGGAGTC GGGATTCCAG GACAAGGAGG AACTGACATG GGAAGATTTT 
CACTTCATGC TGCGGGACCA CAATAGCGAG CTCCGCTTCA CGCAGCTCTG TGTCAAAGGG 
GTGGAGGTGC CTGAAGTCAT CAAGGACCTC TGCCGGCGAG CCTCCTACAT CAGCCAGGAT 
ATGATCTGTC CCTCTCCCAG AGTGAGTGCC CGCTGTTCCC GCAGCGACAT TGAGACTGAG 
TTGACACCTC AGAGACTGCA GTGCCCCATG GACACAGACC CTCCCCAGGA GATTCGGCGG 
AGGTTTGGCA AGAAGGTAAC GTCATTCCAG CCCTTGCTGT TCACTGAGGC GCACCGAGAG 
AAGTTCCAAC GCAGCTGTCT CCACCAGACG GTGCAACAGT TCAAGCGCTT CATTGAGAAC 
TACCGGCGCC ACATCGGCTG CGTGGCCGTG TTCTACGCCA TCGCTGGGGG GCTTTTCCTG 
GAGAGGGCCT ACTACTACGC CTTTGCCGCA CATCACACGG GCATCACAGA CACCACCCGC 
GTGGGAATCA TCCTGTCGCG GGGCACAGCA GCCAGCATCT CTTTCATGTT CTCCTACATC 
TTGCTCACCA TGTGCCGCAA CCTCATCACC TTCCTGCGAG AAACCTTCCT CAACCGCTAC 
GTGCCCTTCG ACGCCGCCGT GGACTTCCAT CGCCTCATTG CCTCCACCGC CATCGTCCTC 
ACAGTCTTAC ACAGTGTGGG CCATGTGGTG AATGTGTACC TGTTCTCCAT CAGCCCCCTC 
AGCGTCCTCT CTTGCCTCTT TCCTGGCCTC TTCCATGATG ATGGGTCTGA GCTCCCCCAG 
AAGTATTACT GGTGGTTCTT CCAGACCGTA CCAGGCCTCA CGGGGGTTGT GCTGCTCCTG 
ATCCTGGCCA TCATGTATGT CTTTGCCTCC CACCACTTCC GCCGCCGCAG TTTCCGGGGC 
TTCTGGCTGA CCCACCACCT CTACATCCTG CTCTATGTCC TGCTCATCAT CCATGGTAGC 
TTTGCCCTGA TCCAGCTGCC CCGTTTCCAC ATCTTCTTCC TGGTCCCAGC AATCATCTAT 
GGGGGCGACA AGCTGGTGAG CCTGAGCCGG AAGAAGGTGG AGATCAGCGT GGTGAAGGCG 
GAGCTGCTGC CCTCAGGAGT GACCCACCTG CGGTTCCAGC GGCCCCAGGG CTTTGAGTAC 
AAGTCAGGGC AGTGGGTGCG GATCGCTTGC CTGGCTCTGG GGACCACCGA GTACCACCCC 
TTCACACTGA CCTCTGCGCC CCATGAGGAC ACGCTTAGCC TGCACATCCG GGCAGCAGGG 
CCCTGGACCA CTCGCCTCAG GGAGATCTAC TCAGCCCCGA CGGGTGACAG ATGTGCCAGA 
TACCCAAAGC TGTACCTTGA TGGACCATTT GGAGAGGGCC ACCAGGAGTG GCATAAGTTT 
GAGGTGTCAG TGTTAGTGGG AGGGGGCATT GGGGTCACCC CTTTTGCCTC CATCCTCAAA 
GACCTGGTCT TCAAGTCATC CGTCAGCTGC CAAGTGTTCT GTAAGAAGAT CTACTTCATC 
TGGGTGACGC GGACCCAGCG TCAGTTTGAG TGGCTGGCTG ACATCATCCG AGAGGTGGAG 
GAGAATGACC ACCAGGACCT GGTGTCTGTG CACATCTACA TCACCCAGCT GGCTGAGAAG 
TTCGACCTCA GGACCACTAT GCTGTACATC TGTGAGCGGC ACTTCCAGAA GGTTCTGAAC 
CGGAGTCTAT TCACAGGCCT GCGCTCCATC ACCCACTTTG GCCGTCCCCC CTTTGAGCCC 
TTCTTCAACT CCCTGCAGGA GGTCCACCCC CAGGTCCGGA AGATCGGGGT GTTTAGCTGT 
GGCCCCCCTG GCATGACCAA GAATGTGGAA AAGGCCTGTC AGCTCATCAA CAGGCAGGAC 
CGGACTCACT TCTCCCACCA TTATGAGAAC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DUOX1 ( XM_011521681.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519983.1 Homo sapiens dual oxidase 1 (DUOX1), transcript variant X1, mRNA.

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CloneID	OHu68237
Clone ID Related Accession (Same CDS sequence)	XM_011521681.2
Accession Version	XM_011521681.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	dual oxidase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521681.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA GATGGGGCAG CAAAGGCTCC CGGCTGCAGC GCCTGGTCCC AGCCAGCTAT 
GCAGATGGCG TGTACCAGCC CTTGGGAGAA CCCCACCTGC CCAACCCCCG AGACCTTAGC 
AACACCATCT CAAGGGGCCC TGCAGGGCTG GCCTCCCTGA GAAACCGCAC AGTGTTGGGG 
GTCTTCTTTG GCTATCACGT GCTTTCAGAC CTGGTGAGCG TGGAAACTCC CGGCTGCCCC 
GCCGAGTTCC TCAACATTCG CATCCCGCCC GGAGACCCCA TGTTCGACCC CGACCAGCGC 
GGGGACGTGG TGCTGCCCTT CCAGAGAAGC CGCTGGGACC CCGAGACCGG ACGGAGTCCC 
AGCAATCCCC GGGACCCGGC CAACCAGGTG ACGGGCTGGC TGGACGGCAG CGCCATCTAT 
GGTTCCTCGC ATTCCTGGAG CGACGCGCTG CGGAGCTTCT CCAGGGGACA GCTGGCGTCG 
GGGCCCGACC CCGCTTTTCC CCGAGACTCG CAGAACCCCC TGCTCATGTG GGCGGCGCCC 
GACCCCGCCA CCGGGCAGAA CGGGCCCCGG GGGCTGTACG CCTTCGGGGC AGAGAGAGGG 
AACCGGGAAC CCTTCCTGCA GGCGCTGGGC CTGCTCTGGT TCCGCTACCA CAACCTGTGG 
GCGCAGAGGC TGGCCCGCCA GCACCCAGAC TGGGAGGACG AGGAGCTGTT CCAGCACGCA 
CGCAAGAGGG TCATCGCCAC CTACCAGAAC ATCGCTGTGT ATGAGTGGCT GCCCAGCTTC 
CTGCAGAAAA CACTCCCGGA GTATACAGGA TACCGGCCAT TTCTGGACCC CAGCATCTCC 
TCAGAGTTCG TGGCGGCCTC TGAGCAGTTC CTGTCCACCA TGGTGCCCCC TGGCGTCTAC 
ATGAGAAATG CCAGCTGCCA CTTCCAGGGG GTCATCAATC GGAACTCAAG TGTCTCCAGA 
GCTCTCCGGG TCTGCAACAG CTACTGGAGC CGTGAGCACC CAAGCCTACA AAGTGCTGAA 
GATGTGGATG CACTGCTGCT GGGCATGGCC TCCCAGATCG CAGAGCGAGA GGACCATGTG 
TTGGTTGAAG ATGTGCGGGA TTTCTGGCCT GGGCCACTGA AGTTTTCCCG CACAGACCAC 
CTGGCCAGCT GCCTGCAGCG GGGCCGGGAT CTGGGCCTGC CCTCTTACAC CAAGGCCAGG 
GCAGCACTGG GCTTGTCTCC CATTACCCGC TGGCAGGACA TCAACCCTGC ACTCTCCCGG 
AGCAATGACA CTGTACTGGA GGCCACAGCT GCCCTGTACA ACCAGGACTT ATCCTGGCTA 
GAGCTGCTCC CTGGGGGACT CCTGGAGAGC CACCGGGACC CTGGACCTCT GTTCAGCACC 
ATCGTCCTTG AACAATTTGT GCGGCTACGG GATGGTGACC GCTACTGGTT TGAGAACACC 
AGGAATGGGC TGTTCTCCAA GAAGGAGATT GAAGAAATCC GAAATACCAC CCTGCAGGAC 
GTGCTGGTCG CTGTTATCAA CATTGACCCC AGTGCTCTGC AGCCCAATGT CTTTGTCTGG 
CATAAAGGAG ACCCCTGTCC GCAGCCGAGA CAGCTCAGCA CTGAAGGCCT GCCAGCGTGT 
GCTCCCTCTG TTGTTCGTGA CTATTTTGAG GGCAGTGGAT TTGGCTTCGG GGTCACCATC 
GGGACCCTCT GTTGCTTCCC TTTGGTGAGC CTGCTCAGTG CCTGGATTGT TGCCCGGCTC 
CGGATGAGAA ATTTCAAGAG GCTCCAGGGC CAGGACCGCC AGAGCATCGT GTCTGAGAAG 
CTCGTGGGAG GCATGGAAGC TTTGGAATGG CAAGGCCACA AGGAGCCCTG CCGGCCCGTG 
CTTGTGTACC TGCAGCCCGG GCAGATCCGT GTGGTAGATG GCAGGCTCAC CGTGCTCCGC 
ACCATCCAGC TGCAGCCTCC ACAGAAGGTC AACTTCGTCC TGTCCAGCAA CCGTGGACGC 
CGCACTCTGC TGCTCAAGAT CCCCAAGGAG TATGACCTGG TGCTGCTGTT TAACTTGGAG 
GAAGAGCGGC AGGCGCTGGT GGAAAATCTC CGGGGAGCTC TGAAGGAGAG CGGGTTGAGC 
ATCCAGGAGT GGGAGCTGCG GGAGCAGGAG CTGATGAGAG CAGCTGTGAC ACGGGAGCAG 
CGGAGGCACC TCCTGGAGAC CTTTTTCAGG CACCTTTTCT CCCAGGTGCT GGACATCAAC 
CAGGCCGACG CAGGGACCCT GCCCCTGGAC TCCTCCCAGA AGGTGCGGGA GGCCCTGACC 
TGTGAGCTGA GCAGGGCCGA GTTTGCCGAG TCCCTGGGCC TCAAGCCCCA GGACATGTTT 
GTGGAGTCCA TGTTCTCTCT GGCTGACAAG GATGGCAATG GCTACCTGTC CTTCCGAGAG 
TTCCTGGACA TCCTGGTGGT CTTCATGAAA GGCTCTCCTG AGGAAAAGTC TCGCCTTATG 
TTCCGCATGT ACGACTTTGA TGGGAATGGC CTCATTTCCA AGGATGAGTT CATCAGGATG 
CTGAGATCCT TCATCGAGAT CTCCAACAAC TGCCTGTCCA AGGCCCAGCT GGCTGAGGTG 
GTGGAGTCCA TGTTCCGGGA GTCGGGATTC CAGGACAAGG AGGAACTGAC ATGGGAAGAT 
TTTCACTTCA TGCTGCGGGA CCACAATAGC GAGCTCCGCT TCACGCAGCT CTGTGTCAAA 
GGGGTGGAGG TGCCTGAAGT CATCAAGGAC CTCTGCCGGC GAGCCTCCTA CATCAGCCAG 
GATATGATCT GTCCCTCTCC CAGAGTGAGT GCCCGCTGTT CCCGCAGCGA CATTGAGACT 
GAGTTGACAC CTCAGAGACT GCAGTGCCCC ATGGACACAG ACCCTCCCCA GGAGATTCGG 
CGGAGGTTTG GCAAGAAGGT AACGTCATTC CAGCCCTTGC TGTTCACTGA GGCGCACCGA 
GAGAAGTTCC AACGCAGCTG TCTCCACCAG ACGGTGCAAC AGTTCAAGCG CTTCATTGAG 
AACTACCGGC GCCACATCGG CTGCGTGGCC GTGTTCTACG CCATCGCTGG GGGGCTTTTC 
CTGGAGAGGG CCTACTACTA CGCCTTTGCC GCACATCACA CGGGCATCAC AGACACCACC 
CGCGTGGGAA TCATCCTGTC GCGGGGCACA GCAGCCAGCA TCTCTTTCAT GTTCTCCTAC 
ATCTTGCTCA CCATGTGCCG CAACCTCATC ACCTTCCTGC GAGAAACCTT CCTCAACCGC 
TACGTGCCCT TCGACGCCGC CGTGGACTTC CATCGCCTCA TTGCCTCCAC CGCCATCGTC 
CTCACAGTCT TACACAGTGT GGGCCATGTG GTGAATGTGT ACCTGTTCTC CATCAGCCCC 
CTCAGCGTCC TCTCTTGCCT CTTTCCTGGC CTCTTCCATG ATGATGGGTC TGAGCTCCCC 
CAGAAGTATT ACTGGTGGTT CTTCCAGACC GTACCAGGCC TCACGGGGGT TGTGCTGCTC 
CTGATCCTGG CCATCATGTA TGTCTTTGCC TCCCACCACT TCCGCCGCCG CAGTTTCCGG 
GGCTTCTGGC TGACCCACCA CCTCTACATC CTGCTCTATG TCCTGCTCAT CATCCATGGT 
AGCTTTGCCC TGATCCAGCT GCCCCGTTTC CACATCTTCT TCCTGGTCCC AGCAATCATC 
TATGGGGGCG ACAAGCTGGT GAGCCTGAGC CGGAAGAAGG TGGAGATCAG CGTGGTGAAG 
GCGGAGCTGC TGCCCTCAGG AGTGACCCAC CTGCGGTTCC AGCGGCCCCA GGGCTTTGAG 
TACAAGTCAG GGCAGTGGGT GCGGATCGCT TGCCTGGCTC TGGGGACCAC CGAGTACCAC 
CCCTTCACAC TGACCTCTGC GCCCCATGAG GACACGCTTA GCCTGCACAT CCGGGCAGCA 
GGGCCCTGGA CCACTCGCCT CAGGGAGATC TACTCAGCCC CGACGGGTGA CAGATGTGCC 
AGATACCCAA AGCTGTACCT TGATGGACCA TTTGGAGAGG GCCACCAGGA GTGGCATAAG 
TTTGAGGTGT CAGTGTTAGT GGGAGGGGGC ATTGGGGTCA CCCCTTTTGC CTCCATCCTC 
AAAGACCTGG TCTTCAAGTC ATCCGTCAGC TGCCAAGTGT TCTGTAAGAA GATCTACTTC 
ATCTGGGTGA CGCGGACCCA GCGTCAGTTT GAGTGGCTGG CTGACATCAT CCGAGAGGTG 
GAGGAGAATG ACCACCAGGA CCTGGTGTCT GTGCACATCT ACATCACCCA GCTGGCTGAG 
AAGTTCGACC TCAGGACCAC TATGCTGTAC ATCTGTGAGC GGCACTTCCA GAAGGTTCTG 
AACCGGAGTC TATTCACAGG CCTGCGCTCC ATCACCCACT TTGGCCGTCC CCCCTTTGAG 
CCCTTCTTCA ACTCCCTGCA GGAGGTCCAC CCCCAGGTCC GGAAGATCGG GGTGTTTAGC 
TGTGGCCCCC CTGGCATGAC CAAGAATGTG GAAAAGGCCT GTCAGCTCAT CAACAGGCAG 
GACCGGACTC ACTTCTCCCA CCATTATGAG AACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519983.1
CDS	165..4703
Translation	MEHRWGSKGSRLQRLVPASYADGVYQPLGEPHLPNPRDLSNTISRGPAGLASLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIRIPPGDPMFDPDQRGDVVLPFQRSRWDPETGRSPSNPRDPANQVTGWLDGSAIYGSSHSWSDALRSFSRGQLASGPDPAFPRDSQNPLLMWAAPDPATGQNGPRGLYAFGAERGNREPFLQALGLLWFRYHNLWAQRLARQHPDWEDEELFQHARKRVIATYQNIAVYEWLPSFLQKTLPEYTGYRPFLDPSISSEFVAASEQFLSTMVPPGVYMRNASCHFQGVINRNSSVSRALRVCNSYWSREHPSLQSAEDVDALLLGMASQIAEREDHVLVEDVRDFWPGPLKFSRTDHLASCLQRGRDLGLPSYTKARAALGLSPITRWQDINPALSRSNDTVLEATAALYNQDLSWLELLPGGLLESHRDPGPLFSTIVLEQFVRLRDGDRYWFENTRNGLFSKKEIEEIRNTTLQDVLVAVINIDPSALQPNVFVWHKGDPCPQPRQLSTEGLPACAPSVVRDYFEGSGFGFGVTIGTLCCFPLVSLLSAWIVARLRMRNFKRLQGQDRQSIVSEKLVGGMEALEWQGHKEPCRPVLVYLQPGQIRVVDGRLTVLRTIQLQPPQKVNFVLSSNRGRRTLLLKIPKEYDLVLLFNLEEERQALVENLRGALKESGLSIQEWELREQELMRAAVTREQRRHLLETFFRHLFSQVLDINQADAGTLPLDSSQKVREALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHNSELRFTQLCVKGVEVPEVIKDLCRRASYISQDMICPSPRVSARCSRSDIETELTPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSCLHQTVQQFKRFIENYRRHIGCVAVFYAIAGGLFLERAYYYAFAAHHTGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRLIASTAIVLTVLHSVGHVVNVYLFSISPLSVLSCLFPGLFHDDGSELPQKYYWWFFQTVPGLTGVVLLLILAIMYVFASHHFRRRSFRGFWLTHHLYILLYVLLIIHGSFALIQLPRFHIFFLVPAIIYGGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAAGPWTTRLREIYSAPTGDRCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDHQDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQDRTHFSHHYENF

Target ORF information:

RefSeq Version	XM_011521681.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dual oxidase 1 (DUOX1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521681.2

ORF Insert Sequence:

ATGGAGCACA GATGGGGCAG CAAAGGCTCC CGGCTGCAGC GCCTGGTCCC AGCCAGCTAT 
GCAGATGGCG TGTACCAGCC CTTGGGAGAA CCCCACCTGC CCAACCCCCG AGACCTTAGC 
AACACCATCT CAAGGGGCCC TGCAGGGCTG GCCTCCCTGA GAAACCGCAC AGTGTTGGGG 
GTCTTCTTTG GCTATCACGT GCTTTCAGAC CTGGTGAGCG TGGAAACTCC CGGCTGCCCC 
GCCGAGTTCC TCAACATTCG CATCCCGCCC GGAGACCCCA TGTTCGACCC CGACCAGCGC 
GGGGACGTGG TGCTGCCCTT CCAGAGAAGC CGCTGGGACC CCGAGACCGG ACGGAGTCCC 
AGCAATCCCC GGGACCCGGC CAACCAGGTG ACGGGCTGGC TGGACGGCAG CGCCATCTAT 
GGTTCCTCGC ATTCCTGGAG CGACGCGCTG CGGAGCTTCT CCAGGGGACA GCTGGCGTCG 
GGGCCCGACC CCGCTTTTCC CCGAGACTCG CAGAACCCCC TGCTCATGTG GGCGGCGCCC 
GACCCCGCCA CCGGGCAGAA CGGGCCCCGG GGGCTGTACG CCTTCGGGGC AGAGAGAGGG 
AACCGGGAAC CCTTCCTGCA GGCGCTGGGC CTGCTCTGGT TCCGCTACCA CAACCTGTGG 
GCGCAGAGGC TGGCCCGCCA GCACCCAGAC TGGGAGGACG AGGAGCTGTT CCAGCACGCA 
CGCAAGAGGG TCATCGCCAC CTACCAGAAC ATCGCTGTGT ATGAGTGGCT GCCCAGCTTC 
CTGCAGAAAA CACTCCCGGA GTATACAGGA TACCGGCCAT TTCTGGACCC CAGCATCTCC 
TCAGAGTTCG TGGCGGCCTC TGAGCAGTTC CTGTCCACCA TGGTGCCCCC TGGCGTCTAC 
ATGAGAAATG CCAGCTGCCA CTTCCAGGGG GTCATCAATC GGAACTCAAG TGTCTCCAGA 
GCTCTCCGGG TCTGCAACAG CTACTGGAGC CGTGAGCACC CAAGCCTACA AAGTGCTGAA 
GATGTGGATG CACTGCTGCT GGGCATGGCC TCCCAGATCG CAGAGCGAGA GGACCATGTG 
TTGGTTGAAG ATGTGCGGGA TTTCTGGCCT GGGCCACTGA AGTTTTCCCG CACAGACCAC 
CTGGCCAGCT GCCTGCAGCG GGGCCGGGAT CTGGGCCTGC CCTCTTACAC CAAGGCCAGG 
GCAGCACTGG GCTTGTCTCC CATTACCCGC TGGCAGGACA TCAACCCTGC ACTCTCCCGG 
AGCAATGACA CTGTACTGGA GGCCACAGCT GCCCTGTACA ACCAGGACTT ATCCTGGCTA 
GAGCTGCTCC CTGGGGGACT CCTGGAGAGC CACCGGGACC CTGGACCTCT GTTCAGCACC 
ATCGTCCTTG AACAATTTGT GCGGCTACGG GATGGTGACC GCTACTGGTT TGAGAACACC 
AGGAATGGGC TGTTCTCCAA GAAGGAGATT GAAGAAATCC GAAATACCAC CCTGCAGGAC 
GTGCTGGTCG CTGTTATCAA CATTGACCCC AGTGCTCTGC AGCCCAATGT CTTTGTCTGG 
CATAAAGGAG ACCCCTGTCC GCAGCCGAGA CAGCTCAGCA CTGAAGGCCT GCCAGCGTGT 
GCTCCCTCTG TTGTTCGTGA CTATTTTGAG GGCAGTGGAT TTGGCTTCGG GGTCACCATC 
GGGACCCTCT GTTGCTTCCC TTTGGTGAGC CTGCTCAGTG CCTGGATTGT TGCCCGGCTC 
CGGATGAGAA ATTTCAAGAG GCTCCAGGGC CAGGACCGCC AGAGCATCGT GTCTGAGAAG 
CTCGTGGGAG GCATGGAAGC TTTGGAATGG CAAGGCCACA AGGAGCCCTG CCGGCCCGTG 
CTTGTGTACC TGCAGCCCGG GCAGATCCGT GTGGTAGATG GCAGGCTCAC CGTGCTCCGC 
ACCATCCAGC TGCAGCCTCC ACAGAAGGTC AACTTCGTCC TGTCCAGCAA CCGTGGACGC 
CGCACTCTGC TGCTCAAGAT CCCCAAGGAG TATGACCTGG TGCTGCTGTT TAACTTGGAG 
GAAGAGCGGC AGGCGCTGGT GGAAAATCTC CGGGGAGCTC TGAAGGAGAG CGGGTTGAGC 
ATCCAGGAGT GGGAGCTGCG GGAGCAGGAG CTGATGAGAG CAGCTGTGAC ACGGGAGCAG 
CGGAGGCACC TCCTGGAGAC CTTTTTCAGG CACCTTTTCT CCCAGGTGCT GGACATCAAC 
CAGGCCGACG CAGGGACCCT GCCCCTGGAC TCCTCCCAGA AGGTGCGGGA GGCCCTGACC 
TGTGAGCTGA GCAGGGCCGA GTTTGCCGAG TCCCTGGGCC TCAAGCCCCA GGACATGTTT 
GTGGAGTCCA TGTTCTCTCT GGCTGACAAG GATGGCAATG GCTACCTGTC CTTCCGAGAG 
TTCCTGGACA TCCTGGTGGT CTTCATGAAA GGCTCTCCTG AGGAAAAGTC TCGCCTTATG 
TTCCGCATGT ACGACTTTGA TGGGAATGGC CTCATTTCCA AGGATGAGTT CATCAGGATG 
CTGAGATCCT TCATCGAGAT CTCCAACAAC TGCCTGTCCA AGGCCCAGCT GGCTGAGGTG 
GTGGAGTCCA TGTTCCGGGA GTCGGGATTC CAGGACAAGG AGGAACTGAC ATGGGAAGAT 
TTTCACTTCA TGCTGCGGGA CCACAATAGC GAGCTCCGCT TCACGCAGCT CTGTGTCAAA 
GGGGTGGAGG TGCCTGAAGT CATCAAGGAC CTCTGCCGGC GAGCCTCCTA CATCAGCCAG 
GATATGATCT GTCCCTCTCC CAGAGTGAGT GCCCGCTGTT CCCGCAGCGA CATTGAGACT 
GAGTTGACAC CTCAGAGACT GCAGTGCCCC ATGGACACAG ACCCTCCCCA GGAGATTCGG 
CGGAGGTTTG GCAAGAAGGT AACGTCATTC CAGCCCTTGC TGTTCACTGA GGCGCACCGA 
GAGAAGTTCC AACGCAGCTG TCTCCACCAG ACGGTGCAAC AGTTCAAGCG CTTCATTGAG 
AACTACCGGC GCCACATCGG CTGCGTGGCC GTGTTCTACG CCATCGCTGG GGGGCTTTTC 
CTGGAGAGGG CCTACTACTA CGCCTTTGCC GCACATCACA CGGGCATCAC AGACACCACC 
CGCGTGGGAA TCATCCTGTC GCGGGGCACA GCAGCCAGCA TCTCTTTCAT GTTCTCCTAC 
ATCTTGCTCA CCATGTGCCG CAACCTCATC ACCTTCCTGC GAGAAACCTT CCTCAACCGC 
TACGTGCCCT TCGACGCCGC CGTGGACTTC CATCGCCTCA TTGCCTCCAC CGCCATCGTC 
CTCACAGTCT TACACAGTGT GGGCCATGTG GTGAATGTGT ACCTGTTCTC CATCAGCCCC 
CTCAGCGTCC TCTCTTGCCT CTTTCCTGGC CTCTTCCATG ATGATGGGTC TGAGCTCCCC 
CAGAAGTATT ACTGGTGGTT CTTCCAGACC GTACCAGGCC TCACGGGGGT TGTGCTGCTC 
CTGATCCTGG CCATCATGTA TGTCTTTGCC TCCCACCACT TCCGCCGCCG CAGTTTCCGG 
GGCTTCTGGC TGACCCACCA CCTCTACATC CTGCTCTATG TCCTGCTCAT CATCCATGGT 
AGCTTTGCCC TGATCCAGCT GCCCCGTTTC CACATCTTCT TCCTGGTCCC AGCAATCATC 
TATGGGGGCG ACAAGCTGGT GAGCCTGAGC CGGAAGAAGG TGGAGATCAG CGTGGTGAAG 
GCGGAGCTGC TGCCCTCAGG AGTGACCCAC CTGCGGTTCC AGCGGCCCCA GGGCTTTGAG 
TACAAGTCAG GGCAGTGGGT GCGGATCGCT TGCCTGGCTC TGGGGACCAC CGAGTACCAC 
CCCTTCACAC TGACCTCTGC GCCCCATGAG GACACGCTTA GCCTGCACAT CCGGGCAGCA 
GGGCCCTGGA CCACTCGCCT CAGGGAGATC TACTCAGCCC CGACGGGTGA CAGATGTGCC 
AGATACCCAA AGCTGTACCT TGATGGACCA TTTGGAGAGG GCCACCAGGA GTGGCATAAG 
TTTGAGGTGT CAGTGTTAGT GGGAGGGGGC ATTGGGGTCA CCCCTTTTGC CTCCATCCTC 
AAAGACCTGG TCTTCAAGTC ATCCGTCAGC TGCCAAGTGT TCTGTAAGAA GATCTACTTC 
ATCTGGGTGA CGCGGACCCA GCGTCAGTTT GAGTGGCTGG CTGACATCAT CCGAGAGGTG 
GAGGAGAATG ACCACCAGGA CCTGGTGTCT GTGCACATCT ACATCACCCA GCTGGCTGAG 
AAGTTCGACC TCAGGACCAC TATGCTGTAC ATCTGTGAGC GGCACTTCCA GAAGGTTCTG 
AACCGGAGTC TATTCACAGG CCTGCGCTCC ATCACCCACT TTGGCCGTCC CCCCTTTGAG 
CCCTTCTTCA ACTCCCTGCA GGAGGTCCAC CCCCAGGTCC GGAAGATCGG GGTGTTTAGC 
TGTGGCCCCC CTGGCATGAC CAAGAATGTG GAAAAGGCCT GTCAGCTCAT CAACAGGCAG 
GACCGGACTC ACTTCTCCCA CCATTATGAG AACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Autophagy regulates DUOX1 localization and superoxide production in airway epithelial cells during chronic IL-13 stimulation.
Redox biology14272-284(2018 Apr)
Dickinson JD,Sweeter JM,Warren KJ,Ahmad IM,De Deken X,Zimmerman MC,Brody SL

Digenic DUOX1 and DUOX2 Mutations in Cases With Congenital Hypothyroidism.
The Journal of clinical endocrinology and metabolism102(9)3085-3090(2017 Sep)
Aycan Z,Cangul H,Muzza M,Bas VN,Fugazzola L,Chatterjee VK,Persani L,Schoenmakers N

Epidermal growth factor-induced hydrogen peroxide production is mediated by dual oxidase 1.
Free radical biology & medicine97204-211(2016 08)
Sirokm?ny G,Pat? A,Zana M,Donk? ?,B?r? A,Nagy P,Geiszt M

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DUOX1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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