ATP6V1E1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP6V1E1
Entrez Gene ID	529
Full Name	ATPase H+ transporting V1 subunit E1
Synonyms	ARCL2C,ATP6E,ATP6E2,ATP6V1E,P31,Vma4
General protein information	Preferred Names ATPase H+ transporting V1 subunit E1 Names V-type proton ATPase subunit E 1 ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 H(+)-transporting two-sector ATPase, 31kDa subunit H+-transporting ATP synthase chain E, vacuolar V-ATPase 31 kDa subunit V-ATPase subunit E 1 V-ATPase, subunit E vacuolar proton pump subunit E 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q11.21
Summary	This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008].
Disorder MIM:	108746
Disorder Html:	Cutis laxa, autosomal recessive, type IIC, 617402 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001039367.1	NP_001034456.1	V-type proton ATPase subunit E 1 isoform c
NM_001039366.1	NP_001034455.1	V-type proton ATPase subunit E 1 isoform b
NM_001696.3	NP_001687.1	V-type proton ATPase subunit E 1 isoform a
NM_001696.4	NP_001687.1	V-type proton ATPase subunit E 1 isoform a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP6V1E1	XP_005637441.1
Rattus norvegicus (Norway rat)	Atp6v1e1	NP_942040.1
Schizosaccharomyces pombe (fission yeast)	vma4	NP_594932.1
Bos taurus (cattle)	ATP6V1E1	NP_777235.1
Neurospora crassa	NCU07446	XP_964633.2
Arabidopsis thaliana (thale cress)	VHA-E2	NP_187468.1
Oryza sativa (rice)	Os05g0480700	NP_001055857.1
Homo sapiens (human)	ATP6V1E1	NP_001687.1
Mus musculus (house mouse)	Atp6v1e1	NP_031536.2
Gallus gallus (chicken)	ATP6V1E1	NP_001006246.1
Danio rerio (zebrafish)	atp6v1e1b	NP_775361.1
Drosophila melanogaster (fruit fly)	Vha26	NP_524237.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002401	XP_312551.1
Caenorhabditis elegans (roundworm)	vha-8	NP_501040.1
Magnaporthe oryzae (rice blast fungus)	MGG_02770	XP_003720979.1
Pan troglodytes (chimpanzee)	ATP6V1E1	XP_514965.2
Saccharomyces cerevisiae (baker's yeast)	VMA4	NP_014977.3
Kluyveromyces lactis	KLLA0F02541g	XP_455195.1
Xenopus tropicalis (tropical clawed frog)	atp6v1e1	NP_989123.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in ATP6V1E1 or ATP6V1A Cause Autosomal-Recessive Cutis Laxa.
Van Damme T, Gardeitchik T, Mohamed M, Guerrero-Castillo S, Freisinger P, Guillemyn B, Kariminejad A, Dalloyaux D, van Kraaij S, Lefeber DJ, Syx D, Steyaert W, De Rycke R, Hoischen A, Kamsteeg EJ, Wong SY, van Scherpenzeel M, Jamali P, Brandt U, Nijtmans L, Korenke GC, Chung BHY, Mak CCY, Hausser I, Kornak U, Fischer-Zirnsak B, Strom TM, Meitinger T, Alanay Y, Utine GE, Leung PKC, Ghaderi-Sohi S, Coucke P, Symoens S, De Paepe A, Thiel C, Haack TB, Malfait F, Morava E, Callewaert B, Wevers RA
American journal of human genetics100(2)216-227(2017 Feb)

Prognostic significance and function of the vacuolar H+-ATPase subunit V1E1 in esophageal squamous cell carcinoma.
Son SW, Kim SH, Moon EY, Kim DH, Pyo S, Um SH
Oncotarget7(31)49334-49348(2016 Aug)

Polarization of the vacuolar adenosine triphosphatase delineates a transition to high-grade pancreatic intraepithelial neoplasm lesions.
Sreekumar BK, Belinsky GS, Einwachter H, Rhim AD, Schmid R, Chung C
Pancreas43(8)1256-63(2014 Nov)

Lipofuscin is formed independently of macroautophagy and lysosomal activity in stress-induced prematurely senescent human fibroblasts.
H?hn A, Sittig A, Jung T, Grimm S, Grune T
Free radical biology & medicine53(9)1760-9(2012 Nov)

A 600?kb triplication in the cat eye syndrome critical region causes anorectal, renal and preauricular anomalies in a three-generation family.
Knijnenburg J, van Bever Y, Hulsman LO, van Kempen CA, Bolman GM, van Loon RL, Beverloo HB, van Zutven LJ
European journal of human genetics : EJHG20(9)986-9(2012 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We report biallelic mutations in ATP6V1E1 and ATP6V1A, respectively encoding the E1 and A subunits of the V1 domain of V-ATPase, as a cause of distinct metabolic and multisystemic cutis laxa entities.
Title: Mutations in ATP6V1E1 or ATP6V1A Cause Autosomal-Recessive Cutis Laxa.

Low-grade PanIN lesions with typical columnar morphology displayed diffuse labeling of the V1E subunit. In advanced lesions it was found along the basolateral membranes.
Title: Polarization of the vacuolar adenosine triphosphatase delineates a transition to high-grade pancreatic intraepithelial neoplasm lesions.

The genes CECR2, SLC25A18 and ATP6V1E1, mapping within the critical region for cat eye syndrome (CES), may be responsible for anorectal, renal and preauricular anomalies in patients with CES.
Title: A 600?kb triplication in the cat eye syndrome critical region causes anorectal, renal and preauricular anomalies in a three-generation family.

HuR shows increased binding to some V-ATPase mRNAs during ATP depletion; siRNA-mediated knockdown of HuR results in diminished V-ATPase expression
Title: HuR stabilizes vacuolar H+-translocating ATPase mRNA during cellular energy depletion.

Data demonstrate the physiological significance of the interaction between the E and H subunits of V-ATPase and extend previous studies on the arrangement of subunits on the peripheral stalk of V-ATPase.
Title: The amino-terminal domain of the E subunit of vacuolar H(+)-ATPase (V-ATPase) interacts with the H subunit and is required for V-ATPase function.

The following ATP6V1E1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP6V1E1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22838	NM_001696.4 Latest version!	Homo sapiens ATPase H+ transporting V1 subunit E1 (ATP6V1E1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu12787	NM_001039366.1 Latest version!	Homo sapiens ATPase H+ transporting V1 subunit E1 (ATP6V1E1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OHu12853	NM_001039367.1 Latest version!	Homo sapiens ATPase H+ transporting V1 subunit E1 (ATP6V1E1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu22838	NM_001696.3	Homo sapiens ATPase H+ transporting V1 subunit E1 (ATP6V1E1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP6V1E1 ( NM_001696.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001687.1 Homo sapiens ATPase H+ transporting V1 subunit E1 (ATP6V1E1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22838
Clone ID Related Accession (Same CDS sequence)	NM_001696.3 , NM_001696.4
Accession Version	NM_001696.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	V-type proton ATPase subunit E 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CK005516.1, AC006285.13, AC007666.12 and KF457350.1. This sequence is a reference standard in the RefSeqGene project. On Feb 10, 2006 this sequence version replaced NM_001696.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.113510.1, SRR1803615.142799.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTCA GCGATGCTGA CGTGCAAAAG CAGATAAAGC ATATGATGGC TTTCATTGAA 
CAAGAAGCCA ATGAGAAAGC AGAAGAAATA GATGCAAAGG CAGAAGAAGA GTTCAACATA 
GAGAAAGGTC GGCTTGTGCA AACCCAAAGA CTAAAGATTA TGGAATATTA TGAGAAGAAA 
GAGAAACAGA TTGAGCAGCA GAAGAAAATT CAGATGTCCA ATTTGATGAA TCAAGCGAGA 
CTCAAAGTCC TCAGAGCAAG AGATGACCTT ATCACAGACC TACTAAATGA AGCAAAACAG 
AGACTCAGCA AGGTGGTAAA AGATACAACC AGGTACCAAG TGCTGCTGGA TGGACTGGTT 
CTCCAGGGTT TGTACCAGTT GCTGGAGCCC CGAATGATTG TTCGTTGCAG GAAACAAGAT 
TTCCCTCTGG TAAAGGCTGC AGTGCAGAAG GCAATTCCTA TGTACAAAAT TGCCACCAAA 
AACGATGTTG ATGTCCAAAT TGACCAGGAG TCCTACCTGC CTGAAGACAT AGCTGGTGGA 
GTTGAGATCT ATAATGGAGA TCGTAAAATA AAGGTTTCCA ACACCCTGGA AAGCCGGCTG 
GATCTCATAG CCCAGCAGAT GATGCCAGAA GTCCGGGGAG CCTTGTTTGG TGCAAATGCC 
AACAGGAAGT TTTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001687.1
CDS	188..868
Translation	MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPEDIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD

Target ORF information:

RefSeq Version	NM_001696.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V1 subunit E1 (ATP6V1E1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001696.3

ORF Insert Sequence:

ATGGCTCTCA GCGATGCTGA CGTGCAAAAG CAGATAAAGC ATATGATGGC TTTCATTGAA 
CAAGAAGCCA ATGAGAAAGC AGAAGAAATA GATGCAAAGG CAGAAGAAGA GTTCAACATA 
GAGAAAGGTC GGCTTGTGCA AACCCAAAGA CTAAAGATTA TGGAATATTA TGAGAAGAAA 
GAGAAACAGA TTGAGCAGCA GAAGAAAATT CAGATGTCCA ATTTGATGAA TCAAGCGAGA 
CTCAAAGTCC TCAGAGCAAG AGATGACCTT ATCACAGACC TACTAAATGA AGCAAAACAG 
AGACTCAGCA AGGTGGTAAA AGATACAACC AGGTACCAAG TGCTGCTGGA TGGACTGGTT 
CTCCAGGGTT TGTACCAGTT GCTGGAGCCC CGAATGATTG TTCGTTGCAG GAAACAAGAT 
TTCCCTCTGG TAAAGGCTGC AGTGCAGAAG GCAATTCCTA TGTACAAAAT TGCCACCAAA 
AACGATGTTG ATGTCCAAAT TGACCAGGAG TCCTACCTGC CTGAAGACAT AGCTGGTGGA 
GTTGAGATCT ATAATGGAGA TCGTAAAATA AAGGTTTCCA ACACCCTGGA AAGCCGGCTG 
GATCTCATAG CCCAGCAGAT GATGCCAGAA GTCCGGGGAG CCTTGTTTGG TGCAAATGCC 
AACAGGAAGT TTTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V1E1 ( NM_001696.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001687.1 Homo sapiens ATPase H+ transporting V1 subunit E1 (ATP6V1E1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22838
Clone ID Related Accession (Same CDS sequence)	NM_001696.3 , NM_001696.4
Accession Version	NM_001696.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	V-type proton ATPase subunit E 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006285.13, AC007666.12 and KF457350.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001696.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189652.201736.1, SRR1803611.250359.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000253413.10/ ENSP00000253413.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCTCA GCGATGCTGA CGTGCAAAAG CAGATAAAGC ATATGATGGC TTTCATTGAA 
CAAGAAGCCA ATGAGAAAGC AGAAGAAATA GATGCAAAGG CAGAAGAAGA GTTCAACATA 
GAGAAAGGTC GGCTTGTGCA AACCCAAAGA CTAAAGATTA TGGAATATTA TGAGAAGAAA 
GAGAAACAGA TTGAGCAGCA GAAGAAAATT CAGATGTCCA ATTTGATGAA TCAAGCGAGA 
CTCAAAGTCC TCAGAGCAAG AGATGACCTT ATCACAGACC TACTAAATGA AGCAAAACAG 
AGACTCAGCA AGGTGGTAAA AGATACAACC AGGTACCAAG TGCTGCTGGA TGGACTGGTT 
CTCCAGGGTT TGTACCAGTT GCTGGAGCCC CGAATGATTG TTCGTTGCAG GAAACAAGAT 
TTCCCTCTGG TAAAGGCTGC AGTGCAGAAG GCAATTCCTA TGTACAAAAT TGCCACCAAA 
AACGATGTTG ATGTCCAAAT TGACCAGGAG TCCTACCTGC CTGAAGACAT AGCTGGTGGA 
GTTGAGATCT ATAATGGAGA TCGTAAAATA AAGGTTTCCA ACACCCTGGA AAGCCGGCTG 
GATCTCATAG CCCAGCAGAT GATGCCAGAA GTCCGGGGAG CCTTGTTTGG TGCAAATGCC 
AACAGGAAGT TTTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001687.1
CDS	115..795
Translation	MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPEDIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD

Target ORF information:

RefSeq Version	NM_001696.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V1 subunit E1 (ATP6V1E1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001696.4

ORF Insert Sequence:

ATGGCTCTCA GCGATGCTGA CGTGCAAAAG CAGATAAAGC ATATGATGGC TTTCATTGAA 
CAAGAAGCCA ATGAGAAAGC AGAAGAAATA GATGCAAAGG CAGAAGAAGA GTTCAACATA 
GAGAAAGGTC GGCTTGTGCA AACCCAAAGA CTAAAGATTA TGGAATATTA TGAGAAGAAA 
GAGAAACAGA TTGAGCAGCA GAAGAAAATT CAGATGTCCA ATTTGATGAA TCAAGCGAGA 
CTCAAAGTCC TCAGAGCAAG AGATGACCTT ATCACAGACC TACTAAATGA AGCAAAACAG 
AGACTCAGCA AGGTGGTAAA AGATACAACC AGGTACCAAG TGCTGCTGGA TGGACTGGTT 
CTCCAGGGTT TGTACCAGTT GCTGGAGCCC CGAATGATTG TTCGTTGCAG GAAACAAGAT 
TTCCCTCTGG TAAAGGCTGC AGTGCAGAAG GCAATTCCTA TGTACAAAAT TGCCACCAAA 
AACGATGTTG ATGTCCAAAT TGACCAGGAG TCCTACCTGC CTGAAGACAT AGCTGGTGGA 
GTTGAGATCT ATAATGGAGA TCGTAAAATA AAGGTTTCCA ACACCCTGGA AAGCCGGCTG 
GATCTCATAG CCCAGCAGAT GATGCCAGAA GTCCGGGGAG CCTTGTTTGG TGCAAATGCC 
AACAGGAAGT TTTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V1E1 ( NM_001039366.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001034455.1 Homo sapiens ATPase H+ transporting V1 subunit E1 (ATP6V1E1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12787
Clone ID Related Accession (Same CDS sequence)	NM_001039366.1
Accession Version	NM_001039366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	V-type proton ATPase subunit E 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CK005516.1, AC006285.13, BQ888820.1 and AC007666.12. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.40316.1, SRR1163655.19829.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMN03267755 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTCA GCGATGCTGA CGTGCAAAAG CAGGCAGAAG AAGAGTTCAA CATAGAGAAA 
GGTCGGCTTG TGCAAACCCA AAGACTAAAG ATTATGGAAT ATTATGAGAA GAAAGAGAAA 
CAGATTGAGC AGCAGAAGAA AATTCAGATG TCCAATTTGA TGAATCAAGC GAGACTCAAA 
GTCCTCAGAG CAAGAGATGA CCTTATCACA GACCTACTAA ATGAAGCAAA ACAGAGACTC 
AGCAAGGTGG TAAAAGATAC AACCAGGTAC CAAGTGCTGC TGGATGGACT GGTTCTCCAG 
GGTTTGTACC AGTTGCTGGA GCCCCGAATG ATTGTTCGTT GCAGGAAACA AGATTTCCCT 
CTGGTAAAGG CTGCAGTGCA GAAGGCAATT CCTATGTACA AAATTGCCAC CAAAAACGAT 
GTTGATGTCC AAATTGACCA GGAGTCCTAC CTGCCTGAAG ACATAGCTGG TGGAGTTGAG 
ATCTATAATG GAGATCGTAA AATAAAGGTT TCCAACACCC TGGAAAGCCG GCTGGATCTC 
ATAGCCCAGC AGATGATGCC AGAAGTCCGG GGAGCCTTGT TTGGTGCAAA TGCCAACAGG 
AAGTTTTTGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034455.1
CDS	188..802
Translation	MALSDADVQKQAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPEDIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD

Target ORF information:

RefSeq Version	NM_001039366.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V1 subunit E1 (ATP6V1E1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039366.1

ORF Insert Sequence:

ATGGCTCTCA GCGATGCTGA CGTGCAAAAG CAGGCAGAAG AAGAGTTCAA CATAGAGAAA 
GGTCGGCTTG TGCAAACCCA AAGACTAAAG ATTATGGAAT ATTATGAGAA GAAAGAGAAA 
CAGATTGAGC AGCAGAAGAA AATTCAGATG TCCAATTTGA TGAATCAAGC GAGACTCAAA 
GTCCTCAGAG CAAGAGATGA CCTTATCACA GACCTACTAA ATGAAGCAAA ACAGAGACTC 
AGCAAGGTGG TAAAAGATAC AACCAGGTAC CAAGTGCTGC TGGATGGACT GGTTCTCCAG 
GGTTTGTACC AGTTGCTGGA GCCCCGAATG ATTGTTCGTT GCAGGAAACA AGATTTCCCT 
CTGGTAAAGG CTGCAGTGCA GAAGGCAATT CCTATGTACA AAATTGCCAC CAAAAACGAT 
GTTGATGTCC AAATTGACCA GGAGTCCTAC CTGCCTGAAG ACATAGCTGG TGGAGTTGAG 
ATCTATAATG GAGATCGTAA AATAAAGGTT TCCAACACCC TGGAAAGCCG GCTGGATCTC 
ATAGCCCAGC AGATGATGCC AGAAGTCCGG GGAGCCTTGT TTGGTGCAAA TGCCAACAGG 
AAGTTTTTGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP6V1E1 ( NM_001039367.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001034456.1 Homo sapiens ATPase H+ transporting V1 subunit E1 (ATP6V1E1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12853
Clone ID Related Accession (Same CDS sequence)	NM_001039367.1
Accession Version	NM_001039367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	V-type proton ATPase subunit E 1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CK005516.1, AC006285.13, BI597419.1, BE735148.1, AW406069.1 and AC007666.12. Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform c), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315941.1, SRR1803615.356399.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTCA GCGATGCTGA CGTGCAAAAG CAGATAAAGC ATATGATGGC TTTCATTGAA 
CAAGAAGCCA ATGAGAAAGC AGAAGAAATA GATGCAAAGG CAGAAGAAGA GTTCAACATA 
GAGAAAGGTC GGCTTGTGCA AACCCAAAGA CTAAAGATTA TGGAATATTA TGAGAAGAAA 
GAGAAACAGA TTGAGCAGCA GAAGAAAATT CAGATGTCCA ATTTGATGAA TCAAGCGAGA 
CTCAAAGTCC TCAGAGCAAG AGATGACCTT ATCACAGGTT TGTACCAGTT GCTGGAGCCC 
CGAATGATTG TTCGTTGCAG GAAACAAGAT TTCCCTCTGG TAAAGGCTGC AGTGCAGAAG 
GCAATTCCTA TGTACAAAAT TGCCACCAAA AACGATGTTG ATGTCCAAAT TGACCAGGAG 
TCCTACCTGC CTGAAGACAT AGCTGGTGGA GTTGAGATCT ATAATGGAGA TCGTAAAATA 
AAGGTTTCCA ACACCCTGGA AAGCCGGCTG GATCTCATAG CCCAGCAGAT GATGCCAGAA 
GTCCGGGGAG CCTTGTTTGG TGCAAATGCC AACAGGAAGT TTTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034456.1
CDS	188..778
Translation	MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKIQMSNLMNQARLKVLRARDDLITGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPEDIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD

Target ORF information:

RefSeq Version	NM_001039367.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATPase H+ transporting V1 subunit E1 (ATP6V1E1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039367.1

ORF Insert Sequence:

ATGGCTCTCA GCGATGCTGA CGTGCAAAAG CAGATAAAGC ATATGATGGC TTTCATTGAA 
CAAGAAGCCA ATGAGAAAGC AGAAGAAATA GATGCAAAGG CAGAAGAAGA GTTCAACATA 
GAGAAAGGTC GGCTTGTGCA AACCCAAAGA CTAAAGATTA TGGAATATTA TGAGAAGAAA 
GAGAAACAGA TTGAGCAGCA GAAGAAAATT CAGATGTCCA ATTTGATGAA TCAAGCGAGA 
CTCAAAGTCC TCAGAGCAAG AGATGACCTT ATCACAGGTT TGTACCAGTT GCTGGAGCCC 
CGAATGATTG TTCGTTGCAG GAAACAAGAT TTCCCTCTGG TAAAGGCTGC AGTGCAGAAG 
GCAATTCCTA TGTACAAAAT TGCCACCAAA AACGATGTTG ATGTCCAAAT TGACCAGGAG 
TCCTACCTGC CTGAAGACAT AGCTGGTGGA GTTGAGATCT ATAATGGAGA TCGTAAAATA 
AAGGTTTCCA ACACCCTGGA AAGCCGGCTG GATCTCATAG CCCAGCAGAT GATGCCAGAA 
GTCCGGGGAG CCTTGTTTGG TGCAAATGCC AACAGGAAGT TTTTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in ATP6V1E1 or ATP6V1A Cause Autosomal-Recessive Cutis Laxa.
American journal of human genetics100(2)216-227(2017 Feb)
Van Damme T,Gardeitchik T,Mohamed M,Guerrero-Castillo S,Freisinger P,Guillemyn B,Kariminejad A,Dalloyaux D,van Kraaij S,Lefeber DJ,Syx D,Steyaert W,De Rycke R,Hoischen A,Kamsteeg EJ,Wong SY,van Scherpenzeel M,Jamali P,Brandt U,Nijtmans L,Korenke GC,Chung BHY,Mak CCY,Hausser I,Kornak U,Fischer-Zirnsak B,Strom TM,Meitinger T,Alanay Y,Utine GE,Leung PKC,Ghaderi-Sohi S,Coucke P,Symoens S,De Paepe A,Thiel C,Haack TB,Malfait F,Morava E,Callewaert B,Wevers RA

Prognostic significance and function of the vacuolar H+-ATPase subunit V1E1 in esophageal squamous cell carcinoma.
Oncotarget7(31)49334-49348(2016 Aug)
Son SW,Kim SH,Moon EY,Kim DH,Pyo S,Um SH

Polarization of the vacuolar adenosine triphosphatase delineates a transition to high-grade pancreatic intraepithelial neoplasm lesions.
Pancreas43(8)1256-63(2014 Nov)
Sreekumar BK,Belinsky GS,Einwachter H,Rhim AD,Schmid R,Chung C

Lipofuscin is formed independently of macroautophagy and lysosomal activity in stress-induced prematurely senescent human fibroblasts.
Free radical biology & medicine53(9)1760-9(2012 Nov)
H?hn A,Sittig A,Jung T,Grimm S,Grune T

A 600?kb triplication in the cat eye syndrome critical region causes anorectal, renal and preauricular anomalies in a three-generation family.
European journal of human genetics : EJHG20(9)986-9(2012 Sep)
Knijnenburg J,van Bever Y,Hulsman LO,van Kempen CA,Bolman GM,van Loon RL,Beverloo HB,van Zutven LJ

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ATP6V1E1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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