PI3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PI3
Entrez Gene ID	5266
Full Name	peptidase inhibitor 3
Synonyms	ESI,SKALP,WAP3,WFDC14,cementoin
General protein information	Preferred Names peptidase inhibitor 3 Names elafin PI-3 WAP four-disulfide core domain 14 WAP four-disulfide core domain protein 14 elastase-specific inhibitor peptidase inhibitor 3, skin-derived pre-elafin protease inhibitor 3, skin-derived (SKALP) protease inhibitor WAP3 skin-derived antileukoproteinase trappin-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q13.12
Summary	This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq, Oct 2014].
Disorder MIM:	182257

mRNA and Protein(s)

mRNA	Protein	Name
NM_002638.3	NP_002629.1	elafin preproprotein
NM_002638.4	NP_002629.1	elafin preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	PI3	NP_002629.1
	PI3	NP_001103981.1
Pan troglodytes (chimpanzee)	PI3	NP_001129323.1
Canis lupus familiaris (dog)	PI3	XP_005635165.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression and Clinical Significance of Elafin in Inflammatory Bowel Disease.
Zhang W, Teng G, Wu T, Tian Y, Wang H
Inflammatory bowel diseases23(12)2134-2141(2017 Dec)

Recombinant human elafin promotes alveologenesis in newborn mice exposed to chronic hyperoxia.
Han W, Li X, Zhang H, Yu B, Guo C, Deng C
The international journal of biochemistry & cell biology92173-182(2017 Nov)

Plasma Neutrophil Elastase and Elafin as Prognostic Biomarker for Acute Respiratory Distress Syndrome: A Multicenter Survival and Longitudinal Prospective Observation Study.
Wang T, Zhu Z, Liu Z, Yi L, Yang Z, Bian W, Chen W, Wang S, Li G, Li A, Martin GS, Zhu X
Shock (Augusta, Ga.)48(2)168-174(2017 Aug)

Pre-elafin is Involved in Ultraviolet-induced Keratinocyte Apoptosis via Pro-caspase-3 Activation Associated with Cystatin-A Down-regulation.
Kim H, Noh M, Bae ON, Lee CH, Lee AY
Acta dermato-venereologica97(5)578-585(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Recombinant human elafin promotes alveologenesis in newborn mice exposed to chronic hyperoxia
Title: Recombinant human elafin promotes alveologenesis in newborn mice exposed to chronic hyperoxia.

significantly higher expression in preterm prelabor ruptured fetal membranes than in membranes from term birth
Title: Anti-inflammatory Elafin in human fetal membranes.

Our study revealed that high elafin levels identified in smoking- and asthma-related microarray data sets and an epidemiologic study significantly reduced the risk of asthma.
Title: Protective effects of elafin against adult asthma.

The elevations in cervical elafin and SLPI expression in the women with preterm delivery might reflect the local response to the pathogen invasion into the cervix preceding preterm labor.
Title: Cervical Expression of Elafin and SLPI in Pregnancy and Their Association With Preterm Labor.

PI3 expression was downregulated in 24% of DCIS and 83% of invasive breast tumors. PI3 was also downregulated in 33% of ovarian cystadenomas, 43% of borderline ovarian tumors, and 86% of invasive ovarian carcinomas.
Title: Elafin is downregulated during breast and ovarian tumorigenesis but its residual expression predicts recurrence.

The following PI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31415	NM_002638.4 Latest version!	Homo sapiens peptidase inhibitor 3 (PI3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu31415	NM_002638.3	Homo sapiens peptidase inhibitor 3 (PI3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PI3 ( NM_002638.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002629.1 Homo sapiens peptidase inhibitor 3 (PI3), mRNA.

Price & Availability↑

CloneID	OHu31415
Clone ID Related Accession (Same CDS sequence)	NM_002638.3 , NM_002638.4
Accession Version	NM_002638.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	elafin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL049767.12, BC010952.1 and AA583773.1. On Oct 7, 2008 this sequence version replaced NM_002638.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG490472.1, BC010952.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 19906197 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGGCCA GCAGCTTCTT GATCGTGGTG GTGTTCCTCA TCGCTGGGAC GCTGGTTCTA 
GAGGCAGCTG TCACGGGAGT TCCTGTTAAA GGTCAAGACA CTGTCAAAGG CCGTGTTCCA 
TTCAATGGAC AAGATCCCGT TAAAGGACAA GTTTCAGTTA AAGGTCAAGA TAAAGTCAAA 
GCGCAAGAGC CAGTCAAAGG TCCAGTCTCC ACTAAGCCTG GCTCCTGCCC CATTATCTTG 
ATCCGGTGCG CCATGTTGAA TCCCCCTAAC CGCTGCTTGA AAGATACTGA CTGCCCAGGA 
ATCAAGAAGT GCTGTGAAGG CTCTTGCGGG ATGGCCTGTT TCGTTCCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002629.1
CDS	25..378
Translation	MRASSFLIVVVFLIAGTLVLEAAVTGVPVKGQDTVKGRVPFNGQDPVKGQVSVKGQDKVKAQEPVKGPVSTKPGSCPIILIRCAMLNPPNRCLKDTDCPGIKKCCEGSCGMACFVPQ

Target ORF information:

RefSeq Version	NM_002638.3
Organism	Homo sapiens(human)
Definition	Homo sapiens peptidase inhibitor 3 (PI3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002638.3

ORF Insert Sequence:

ATGAGGGCCA GCAGCTTCTT GATCGTGGTG GTGTTCCTCA TCGCTGGGAC GCTGGTTCTA 
GAGGCAGCTG TCACGGGAGT TCCTGTTAAA GGTCAAGACA CTGTCAAAGG CCGTGTTCCA 
TTCAATGGAC AAGATCCCGT TAAAGGACAA GTTTCAGTTA AAGGTCAAGA TAAAGTCAAA 
GCGCAAGAGC CAGTCAAAGG TCCAGTCTCC ACTAAGCCTG GCTCCTGCCC CATTATCTTG 
ATCCGGTGCG CCATGTTGAA TCCCCCTAAC CGCTGCTTGA AAGATACTGA CTGCCCAGGA 
ATCAAGAAGT GCTGTGAAGG CTCTTGCGGG ATGGCCTGTT TCGTTCCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PI3 ( NM_002638.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002629.1 Homo sapiens peptidase inhibitor 3 (PI3), mRNA.

Price & Availability↑

CloneID	OHu31415
Clone ID Related Accession (Same CDS sequence)	NM_002638.3 , NM_002638.4
Accession Version	NM_002638.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Homo sapiens(human)
Product	elafin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL049767.12, BC010952.1 and AA583773.1. On Nov 22, 2018 this sequence version replaced NM_002638.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG490472.1, BC010952.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000243924.4/ ENSP00000243924.3 Protein has antimicrobial activity :: PMID: 19906197 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGGCCA GCAGCTTCTT GATCGTGGTG GTGTTCCTCA TCGCTGGGAC GCTGGTTCTA 
GAGGCAGCTG TCACGGGAGT TCCTGTTAAA GGTCAAGACA CTGTCAAAGG CCGTGTTCCA 
TTCAATGGAC AAGATCCCGT TAAAGGACAA GTTTCAGTTA AAGGTCAAGA TAAAGTCAAA 
GCGCAAGAGC CAGTCAAAGG TCCAGTCTCC ACTAAGCCTG GCTCCTGCCC CATTATCTTG 
ATCCGGTGCG CCATGTTGAA TCCCCCTAAC CGCTGCTTGA AAGATACTGA CTGCCCAGGA 
ATCAAGAAGT GCTGTGAAGG CTCTTGCGGG ATGGCCTGTT TCGTTCCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002629.1
CDS	22..375
Translation	MRASSFLIVVVFLIAGTLVLEAAVTGVPVKGQDTVKGRVPFNGQDPVKGQVSVKGQDKVKAQEPVKGPVSTKPGSCPIILIRCAMLNPPNRCLKDTDCPGIKKCCEGSCGMACFVPQ

Target ORF information:

RefSeq Version	NM_002638.4
Organism	Homo sapiens(human)
Definition	Homo sapiens peptidase inhibitor 3 (PI3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002638.4

ORF Insert Sequence:

ATGAGGGCCA GCAGCTTCTT GATCGTGGTG GTGTTCCTCA TCGCTGGGAC GCTGGTTCTA 
GAGGCAGCTG TCACGGGAGT TCCTGTTAAA GGTCAAGACA CTGTCAAAGG CCGTGTTCCA 
TTCAATGGAC AAGATCCCGT TAAAGGACAA GTTTCAGTTA AAGGTCAAGA TAAAGTCAAA 
GCGCAAGAGC CAGTCAAAGG TCCAGTCTCC ACTAAGCCTG GCTCCTGCCC CATTATCTTG 
ATCCGGTGCG CCATGTTGAA TCCCCCTAAC CGCTGCTTGA AAGATACTGA CTGCCCAGGA 
ATCAAGAAGT GCTGTGAAGG CTCTTGCGGG ATGGCCTGTT TCGTTCCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression and Clinical Significance of Elafin in Inflammatory Bowel Disease.
Inflammatory bowel diseases23(12)2134-2141(2017 Dec)
Zhang W,Teng G,Wu T,Tian Y,Wang H

Recombinant human elafin promotes alveologenesis in newborn mice exposed to chronic hyperoxia.
The international journal of biochemistry & cell biology92173-182(2017 Nov)
Han W,Li X,Zhang H,Yu B,Guo C,Deng C

Plasma Neutrophil Elastase and Elafin as Prognostic Biomarker for Acute Respiratory Distress Syndrome: A Multicenter Survival and Longitudinal Prospective Observation Study.
Shock (Augusta, Ga.)48(2)168-174(2017 Aug)
Wang T,Zhu Z,Liu Z,Yi L,Yang Z,Bian W,Chen W,Wang S,Li G,Li A,Martin GS,Zhu X

Pre-elafin is Involved in Ultraviolet-induced Keratinocyte Apoptosis via Pro-caspase-3 Activation Associated with Cystatin-A Down-regulation.
Acta dermato-venereologica97(5)578-585(2017 May)
Kim H,Noh M,Bae ON,Lee CH,Lee AY