PHKG2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHKG2
Entrez Gene ID	5261
Full Name	phosphorylase kinase catalytic subunit gamma 2
Synonyms	GSD9C
General protein information	Preferred Names phosphorylase kinase catalytic subunit gamma 2 Names phosphorylase b kinase gamma catalytic chain, liver/testis isoform PHK-gamma-LT PHK-gamma-T PSK-C3 Phosphorylase kinase, gamma 2 (testis/liver) phosphorylase b kinase gamma catalytic chain, testis/liver isoform phosphorylase kinase gamma subunit 2 phosphorylase kinase subunit gamma-2 phosphorylase kinase, gamma 2 (testis) serine/threonine-protein kinase PHKG2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p11.2
Summary	Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010].
Disorder MIM:	172471
Disorder Html:	Glycogen storage disease IXc, 613027 (3); Cirrhosis due to liver phosphorylase kinase deficiency (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000294.3	NP_000285.1	phosphorylase b kinase gamma catalytic chain, liver/testis isoform isoform 1
NM_000294.2	NP_000285.1	phosphorylase b kinase gamma catalytic chain, liver/testis isoform isoform 1
NM_001172432.1	NP_001165903.1	phosphorylase b kinase gamma catalytic chain, liver/testis isoform isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PHKG2	NP_000285.1
Bos taurus (cattle)	PHKG2	NP_001039593.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002647	XP_312280.3
Danio rerio (zebrafish)	phkg2	NP_957256.1
Pan troglodytes (chimpanzee)	PHKG2	XP_001144282.2
Canis lupus familiaris (dog)	PHKG2	XP_005621329.1
Drosophila melanogaster (fruit fly)	PhKgamma	NP_727548.1
	LOC100423096	XP_002802499.1
Mus musculus (house mouse)	Phkg2	NP_081164.2
Caenorhabditis elegans (roundworm)	Y50D7A.3	NP_497181.2

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

Novel PHKG2 mutation causing GSD IX with prominent liver disease: report of three cases and review of literature.
Albash B, Imtiaz F, Al-Zaidan H, Al-Manea H, Banemai M, Allam R, Al-Suheel A, Al-Owain M
European journal of pediatrics173(5)647-53(2014 May)

Variability of disease spectrum in children with liver phosphorylase kinase deficiency caused by mutations in the PHKG2 gene.
Bali DS, Goldstein JL, Fredrickson K, Rehder C, Boney A, Austin S, Weinstein DA, Lutz R, Boneh A, Kishnani PS
Molecular genetics and metabolism111(3)309-313(2014 Mar)

Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS.
Varjosalo M, Sacco R, Stukalov A, van Drogen A, Planyavsky M, Hauri S, Aebersold R, Bennett KL, Colinge J, Gstaiger M, Superti-Furga G
Nature methods10(4)307-14(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Patients with PHKG2 mutations have a severe hepatic phenotype within the heterogeneous GSD IX disorder. A defect in PHKG2 should be considered in patients with suspected glycogenosis associated with significant liver fibrosis and cirrhosis.
Title: Novel PHKG2 mutation causing GSD IX with prominent liver disease: report of three cases and review of literature.

These cases add to the current knowledge of clinical variability in patients with PHKG2 mutations. Long term studies, involving follow-up of these patients into adulthood, are needed
Title: Variability of disease spectrum in children with liver phosphorylase kinase deficiency caused by mutations in the PHKG2 gene.

PHKG2 mutations are associated with Glycogen storage disease type IX
Title: Glycogen storage disease type IX: High variability in clinical phenotype.

The following PHKG2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHKG2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23652	NM_000294.3 Latest version!	Homo sapiens phosphorylase kinase catalytic subunit gamma 2 (PHKG2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu21036	NM_001172432.1 Latest version!	Homo sapiens phosphorylase kinase catalytic subunit gamma 2 (PHKG2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu23652	NM_000294.2	Homo sapiens phosphorylase kinase catalytic subunit gamma 2 (PHKG2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHKG2 ( NM_000294.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000285.1 Homo sapiens phosphorylase kinase catalytic subunit gamma 2 (PHKG2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23652
Clone ID Related Accession (Same CDS sequence)	NM_000294.3 , NM_000294.2
Accession Version	NM_000294.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	phosphorylase b kinase gamma catalytic chain, liver/testis isoform isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106886.3. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000294.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.242401.1, SRR1803615.213690.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000563588.6/ ENSP00000455607.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACGCTGG ACGTGGGGCC GGAGGATGAG CTGCCCGACT GGGCCGCCGC CAAAGAGTTT 
TACCAGAAGT ACGACCCTAA GGACGTCATC GGCAGAGGAG TGAGCTCTGT GGTCCGCCGT 
TGTGTTCATC GAGCTACTGG CCACGAGTTT GCGGTGAAGA TTATGGAAGT GACAGCTGAG 
CGGCTGAGTC CTGAGCAGCT GGAGGAGGTG CGGGAAGCCA CACGGCGAGA GACACACATC 
CTTCGCCAGG TCGCCGGCCA CCCCCACATC ATCACCCTCA TCGATTCCTA CGAGTCTTCT 
AGCTTCATGT TCCTGGTGTT TGACCTGATG CGGAAGGGAG AGCTGTTTGA CTATCTCACA 
GAGAAGGTGG CCCTCTCTGA AAAGGAAACC AGGTCCATCA TGCGGTCTCT GCTGGAAGCA 
GTGAGCTTTC TCCATGCCAA CAACATTGTG CATCGAGATC TGAAGCCCGA GAATATTCTC 
CTAGATGACA ATATGCAGAT CCGACTTTCA GATTTCGGGT TCTCCTGCCA CTTGGAACCT 
GGCGAGAAGC TTCGAGAGTT GTGTGGGACC CCAGGGTATC TAGCGCCAGA GATCCTTAAA 
TGCTCCATGG ATGAAACCCA CCCAGGCTAT GGCAAGGAGG TCGACCTCTG GGCCTGTGGG 
GTGATCTTGT TCACACTCCT GGCTGGCTCG CCACCCTTCT GGCACCGGCG GCAGATCCTG 
ATGTTACGCA TGATCATGGA GGGCCAGTAC CAGTTCAGTT CCCCCGAGTG GGATGACCGT 
TCCAGCACTG TCAAAGACCT GATCTCCAGG CTGCTGCAGG TGGATCCTGA GGCACGCCTG 
ACAGCTGAGC AGGCCCTACA GCACCCCTTC TTTGAGCGTT GTGAAGGCAG CCAACCCTGG 
AACCTCACCC CCCGCCAGCG GTTCCGGGTG GCAGTGTGGA CAGTGCTGGC TGCTGGACGA 
GTGGCCCTAA GCACCCATCG TGTACGGCCA CTGACCAAGA ATGCACTGTT GAGGGACCCT 
TATGCGCTGC GGTCAGTGCG GCACCTCATC GACAACTGTG CCTTCCGGCT CTACGGGCAC 
TGGGTAAAGA AAGGGGAGCA GCAGAACCGG GCGGCTCTCT TTCAGCACCG GCCCCCTGGG 
CCTTTTCCCA TCATGGGCCC TGAAGAGGAG GGAGACTCTG CTGCTATAAC TGAGGATGAG 
GCCGTGCTTG TGCTGGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000285.1
CDS	85..1305
Translation	MTLDVGPEDELPDWAAAKEFYQKYDPKDVIGRGVSSVVRRCVHRATGHEFAVKIMEVTAERLSPEQLEEVREATRRETHILRQVAGHPHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKVALSEKETRSIMRSLLEAVSFLHANNIVHRDLKPENILLDDNMQIRLSDFGFSCHLEPGEKLRELCGTPGYLAPEILKCSMDETHPGYGKEVDLWACGVILFTLLAGSPPFWHRRQILMLRMIMEGQYQFSSPEWDDRSSTVKDLISRLLQVDPEARLTAEQALQHPFFERCEGSQPWNLTPRQRFRVAVWTVLAAGRVALSTHRVRPLTKNALLRDPYALRSVRHLIDNCAFRLYGHWVKKGEQQNRAALFQHRPPGPFPIMGPEEEGDSAAITEDEAVLVLG

Target ORF information:

RefSeq Version	NM_000294.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphorylase kinase catalytic subunit gamma 2 (PHKG2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000294.3

ORF Insert Sequence:

ATGACGCTGG ACGTGGGGCC GGAGGATGAG CTGCCCGACT GGGCCGCCGC CAAAGAGTTT 
TACCAGAAGT ACGACCCTAA GGACGTCATC GGCAGAGGAG TGAGCTCTGT GGTCCGCCGT 
TGTGTTCATC GAGCTACTGG CCACGAGTTT GCGGTGAAGA TTATGGAAGT GACAGCTGAG 
CGGCTGAGTC CTGAGCAGCT GGAGGAGGTG CGGGAAGCCA CACGGCGAGA GACACACATC 
CTTCGCCAGG TCGCCGGCCA CCCCCACATC ATCACCCTCA TCGATTCCTA CGAGTCTTCT 
AGCTTCATGT TCCTGGTGTT TGACCTGATG CGGAAGGGAG AGCTGTTTGA CTATCTCACA 
GAGAAGGTGG CCCTCTCTGA AAAGGAAACC AGGTCCATCA TGCGGTCTCT GCTGGAAGCA 
GTGAGCTTTC TCCATGCCAA CAACATTGTG CATCGAGATC TGAAGCCCGA GAATATTCTC 
CTAGATGACA ATATGCAGAT CCGACTTTCA GATTTCGGGT TCTCCTGCCA CTTGGAACCT 
GGCGAGAAGC TTCGAGAGTT GTGTGGGACC CCAGGGTATC TAGCGCCAGA GATCCTTAAA 
TGCTCCATGG ATGAAACCCA CCCAGGCTAT GGCAAGGAGG TCGACCTCTG GGCCTGTGGG 
GTGATCTTGT TCACACTCCT GGCTGGCTCG CCACCCTTCT GGCACCGGCG GCAGATCCTG 
ATGTTACGCA TGATCATGGA GGGCCAGTAC CAGTTCAGTT CCCCCGAGTG GGATGACCGT 
TCCAGCACTG TCAAAGACCT GATCTCCAGG CTGCTGCAGG TGGATCCTGA GGCACGCCTG 
ACAGCTGAGC AGGCCCTACA GCACCCCTTC TTTGAGCGTT GTGAAGGCAG CCAACCCTGG 
AACCTCACCC CCCGCCAGCG GTTCCGGGTG GCAGTGTGGA CAGTGCTGGC TGCTGGACGA 
GTGGCCCTAA GCACCCATCG TGTACGGCCA CTGACCAAGA ATGCACTGTT GAGGGACCCT 
TATGCGCTGC GGTCAGTGCG GCACCTCATC GACAACTGTG CCTTCCGGCT CTACGGGCAC 
TGGGTAAAGA AAGGGGAGCA GCAGAACCGG GCGGCTCTCT TTCAGCACCG GCCCCCTGGG 
CCTTTTCCCA TCATGGGCCC TGAAGAGGAG GGAGACTCTG CTGCTATAAC TGAGGATGAG 
GCCGTGCTTG TGCTGGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKG2 ( NM_000294.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000285.1 Homo sapiens phosphorylase kinase catalytic subunit gamma 2 (PHKG2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23652
Clone ID Related Accession (Same CDS sequence)	NM_000294.3 , NM_000294.2
Accession Version	NM_000294.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	phosphorylase b kinase gamma catalytic chain, liver/testis isoform isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106886.3 and M31606.1. This sequence is a reference standard in the RefSeqGene project. On Feb 18, 2010 this sequence version replaced NM_000294.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.213690.1, SRR1660807.76650.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGACGCTGG ACGTGGGGCC GGAGGATGAG CTGCCCGACT GGGCCGCCGC CAAAGAGTTT 
TACCAGAAGT ACGACCCTAA GGACGTCATC GGCAGAGGAG TGAGCTCTGT GGTCCGCCGT 
TGTGTTCATC GAGCTACTGG CCACGAGTTT GCGGTGAAGA TTATGGAAGT GACAGCTGAG 
CGGCTGAGTC CTGAGCAGCT GGAGGAGGTG CGGGAAGCCA CACGGCGAGA GACACACATC 
CTTCGCCAGG TCGCCGGCCA CCCCCACATC ATCACCCTCA TCGATTCCTA CGAGTCTTCT 
AGCTTCATGT TCCTGGTGTT TGACCTGATG CGGAAGGGAG AGCTGTTTGA CTATCTCACA 
GAGAAGGTGG CCCTCTCTGA AAAGGAAACC AGGTCCATCA TGCGGTCTCT GCTGGAAGCA 
GTGAGCTTTC TCCATGCCAA CAACATTGTG CATCGAGATC TGAAGCCCGA GAATATTCTC 
CTAGATGACA ATATGCAGAT CCGACTTTCA GATTTCGGGT TCTCCTGCCA CTTGGAACCT 
GGCGAGAAGC TTCGAGAGTT GTGTGGGACC CCAGGGTATC TAGCGCCAGA GATCCTTAAA 
TGCTCCATGG ATGAAACCCA CCCAGGCTAT GGCAAGGAGG TCGACCTCTG GGCCTGTGGG 
GTGATCTTGT TCACACTCCT GGCTGGCTCG CCACCCTTCT GGCACCGGCG GCAGATCCTG 
ATGTTACGCA TGATCATGGA GGGCCAGTAC CAGTTCAGTT CCCCCGAGTG GGATGACCGT 
TCCAGCACTG TCAAAGACCT GATCTCCAGG CTGCTGCAGG TGGATCCTGA GGCACGCCTG 
ACAGCTGAGC AGGCCCTACA GCACCCCTTC TTTGAGCGTT GTGAAGGCAG CCAACCCTGG 
AACCTCACCC CCCGCCAGCG GTTCCGGGTG GCAGTGTGGA CAGTGCTGGC TGCTGGACGA 
GTGGCCCTAA GCACCCATCG TGTACGGCCA CTGACCAAGA ATGCACTGTT GAGGGACCCT 
TATGCGCTGC GGTCAGTGCG GCACCTCATC GACAACTGTG CCTTCCGGCT CTACGGGCAC 
TGGGTAAAGA AAGGGGAGCA GCAGAACCGG GCGGCTCTCT TTCAGCACCG GCCCCCTGGG 
CCTTTTCCCA TCATGGGCCC TGAAGAGGAG GGAGACTCTG CTGCTATAAC TGAGGATGAG 
GCCGTGCTTG TGCTGGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000285.1
CDS	211..1431
Translation	MTLDVGPEDELPDWAAAKEFYQKYDPKDVIGRGVSSVVRRCVHRATGHEFAVKIMEVTAERLSPEQLEEVREATRRETHILRQVAGHPHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKVALSEKETRSIMRSLLEAVSFLHANNIVHRDLKPENILLDDNMQIRLSDFGFSCHLEPGEKLRELCGTPGYLAPEILKCSMDETHPGYGKEVDLWACGVILFTLLAGSPPFWHRRQILMLRMIMEGQYQFSSPEWDDRSSTVKDLISRLLQVDPEARLTAEQALQHPFFERCEGSQPWNLTPRQRFRVAVWTVLAAGRVALSTHRVRPLTKNALLRDPYALRSVRHLIDNCAFRLYGHWVKKGEQQNRAALFQHRPPGPFPIMGPEEEGDSAAITEDEAVLVLG

Target ORF information:

RefSeq Version	NM_000294.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphorylase kinase catalytic subunit gamma 2 (PHKG2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000294.2

ORF Insert Sequence:

ATGACGCTGG ACGTGGGGCC GGAGGATGAG CTGCCCGACT GGGCCGCCGC CAAAGAGTTT 
TACCAGAAGT ACGACCCTAA GGACGTCATC GGCAGAGGAG TGAGCTCTGT GGTCCGCCGT 
TGTGTTCATC GAGCTACTGG CCACGAGTTT GCGGTGAAGA TTATGGAAGT GACAGCTGAG 
CGGCTGAGTC CTGAGCAGCT GGAGGAGGTG CGGGAAGCCA CACGGCGAGA GACACACATC 
CTTCGCCAGG TCGCCGGCCA CCCCCACATC ATCACCCTCA TCGATTCCTA CGAGTCTTCT 
AGCTTCATGT TCCTGGTGTT TGACCTGATG CGGAAGGGAG AGCTGTTTGA CTATCTCACA 
GAGAAGGTGG CCCTCTCTGA AAAGGAAACC AGGTCCATCA TGCGGTCTCT GCTGGAAGCA 
GTGAGCTTTC TCCATGCCAA CAACATTGTG CATCGAGATC TGAAGCCCGA GAATATTCTC 
CTAGATGACA ATATGCAGAT CCGACTTTCA GATTTCGGGT TCTCCTGCCA CTTGGAACCT 
GGCGAGAAGC TTCGAGAGTT GTGTGGGACC CCAGGGTATC TAGCGCCAGA GATCCTTAAA 
TGCTCCATGG ATGAAACCCA CCCAGGCTAT GGCAAGGAGG TCGACCTCTG GGCCTGTGGG 
GTGATCTTGT TCACACTCCT GGCTGGCTCG CCACCCTTCT GGCACCGGCG GCAGATCCTG 
ATGTTACGCA TGATCATGGA GGGCCAGTAC CAGTTCAGTT CCCCCGAGTG GGATGACCGT 
TCCAGCACTG TCAAAGACCT GATCTCCAGG CTGCTGCAGG TGGATCCTGA GGCACGCCTG 
ACAGCTGAGC AGGCCCTACA GCACCCCTTC TTTGAGCGTT GTGAAGGCAG CCAACCCTGG 
AACCTCACCC CCCGCCAGCG GTTCCGGGTG GCAGTGTGGA CAGTGCTGGC TGCTGGACGA 
GTGGCCCTAA GCACCCATCG TGTACGGCCA CTGACCAAGA ATGCACTGTT GAGGGACCCT 
TATGCGCTGC GGTCAGTGCG GCACCTCATC GACAACTGTG CCTTCCGGCT CTACGGGCAC 
TGGGTAAAGA AAGGGGAGCA GCAGAACCGG GCGGCTCTCT TTCAGCACCG GCCCCCTGGG 
CCTTTTCCCA TCATGGGCCC TGAAGAGGAG GGAGACTCTG CTGCTATAAC TGAGGATGAG 
GCCGTGCTTG TGCTGGGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKG2 ( NM_001172432.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165903.1 Homo sapiens phosphorylase kinase catalytic subunit gamma 2 (PHKG2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21036
Clone ID Related Accession (Same CDS sequence)	NM_001172432.1
Accession Version	NM_001172432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	phosphorylase b kinase gamma catalytic chain, liver/testis isoform isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106886.3, AK293551.1 and CA449954.1. Transcript Variant: This variant (2) lacks an internal segment in the 3' region, as compared to variant 1. The resulting isoform (2) is shorter and has a different C-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293551.1, SRR1803615.97112.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END##

ATGACGCTGG ACGTGGGGCC GGAGGATGAG CTGCCCGACT GGGCCGCCGC CAAAGAGTTT 
TACCAGAAGT ACGACCCTAA GGACGTCATC GGCAGAGGAG TGAGCTCTGT GGTCCGCCGT 
TGTGTTCATC GAGCTACTGG CCACGAGTTT GCGGTGAAGA TTATGGAAGT GACAGCTGAG 
CGGCTGAGTC CTGAGCAGCT GGAGGAGGTG CGGGAAGCCA CACGGCGAGA GACACACATC 
CTTCGCCAGG TCGCCGGCCA CCCCCACATC ATCACCCTCA TCGATTCCTA CGAGTCTTCT 
AGCTTCATGT TCCTGGTGTT TGACCTGATG CGGAAGGGAG AGCTGTTTGA CTATCTCACA 
GAGAAGGTGG CCCTCTCTGA AAAGGAAACC AGGTCCATCA TGCGGTCTCT GCTGGAAGCA 
GTGAGCTTTC TCCATGCCAA CAACATTGTG CATCGAGATC TGAAGCCCGA GAATATTCTC 
CTAGATGACA ATATGCAGAT CCGACTTTCA GATTTCGGGT TCTCCTGCCA CTTGGAACCT 
GGCGAGAAGC TTCGAGAGTT GTGTGGGACC CCAGGGTATC TAGCGCCAGA GATCCTTAAA 
TGCTCCATGG ATGAAACCCA CCCAGGCTAT GGCAAGGAGG TCGACCTCTG GGCCTGTGGG 
GTGATCTTGT TCACACTCCT GGCTGGCTCG CCACCCTTCT GGCACCGGCG GCAGATCCTG 
ATGTTACGCA TGATCATGGA GGGCCAGTAC CAGTTCAGTT CCCCCGAGTG GGATGACCGT 
TCCAGCACTG TCAAAGACCT GATCTCCAGG CTGCTGCAGG TGGATCCTGA GGCACGCCTG 
ACAGCTGAGC AGGCCCTACA GCACCCCTTC TTTGAGCGTT GTGAAGGCAG CCAACCCTGG 
AACCTCACCC CCCGCCAGCG GTTCCGGGTG GCAGTGTGGA CAGTGCTGGC TGCTGGACGA 
GTGGCCCTAA GCACCCATCG TGTACGGCCA CTGACCAAGA ATGCACTGTT GAGGGACCCT 
TATGCGCTGC GGTCAGTGCG GCACCTCATC GACAACTGTG CCTTCCGGCT CTACGGGCAC 
TGGATAAGGA AGCAGTGGAT TGGAAAGCTG ATGGCTTGTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165903.1
CDS	211..1335
Translation	MTLDVGPEDELPDWAAAKEFYQKYDPKDVIGRGVSSVVRRCVHRATGHEFAVKIMEVTAERLSPEQLEEVREATRRETHILRQVAGHPHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKVALSEKETRSIMRSLLEAVSFLHANNIVHRDLKPENILLDDNMQIRLSDFGFSCHLEPGEKLRELCGTPGYLAPEILKCSMDETHPGYGKEVDLWACGVILFTLLAGSPPFWHRRQILMLRMIMEGQYQFSSPEWDDRSSTVKDLISRLLQVDPEARLTAEQALQHPFFERCEGSQPWNLTPRQRFRVAVWTVLAAGRVALSTHRVRPLTKNALLRDPYALRSVRHLIDNCAFRLYGHWIRKQWIGKLMACV

Target ORF information:

RefSeq Version	NM_001172432.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphorylase kinase catalytic subunit gamma 2 (PHKG2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172432.1

ORF Insert Sequence:

ATGACGCTGG ACGTGGGGCC GGAGGATGAG CTGCCCGACT GGGCCGCCGC CAAAGAGTTT 
TACCAGAAGT ACGACCCTAA GGACGTCATC GGCAGAGGAG TGAGCTCTGT GGTCCGCCGT 
TGTGTTCATC GAGCTACTGG CCACGAGTTT GCGGTGAAGA TTATGGAAGT GACAGCTGAG 
CGGCTGAGTC CTGAGCAGCT GGAGGAGGTG CGGGAAGCCA CACGGCGAGA GACACACATC 
CTTCGCCAGG TCGCCGGCCA CCCCCACATC ATCACCCTCA TCGATTCCTA CGAGTCTTCT 
AGCTTCATGT TCCTGGTGTT TGACCTGATG CGGAAGGGAG AGCTGTTTGA CTATCTCACA 
GAGAAGGTGG CCCTCTCTGA AAAGGAAACC AGGTCCATCA TGCGGTCTCT GCTGGAAGCA 
GTGAGCTTTC TCCATGCCAA CAACATTGTG CATCGAGATC TGAAGCCCGA GAATATTCTC 
CTAGATGACA ATATGCAGAT CCGACTTTCA GATTTCGGGT TCTCCTGCCA CTTGGAACCT 
GGCGAGAAGC TTCGAGAGTT GTGTGGGACC CCAGGGTATC TAGCGCCAGA GATCCTTAAA 
TGCTCCATGG ATGAAACCCA CCCAGGCTAT GGCAAGGAGG TCGACCTCTG GGCCTGTGGG 
GTGATCTTGT TCACACTCCT GGCTGGCTCG CCACCCTTCT GGCACCGGCG GCAGATCCTG 
ATGTTACGCA TGATCATGGA GGGCCAGTAC CAGTTCAGTT CCCCCGAGTG GGATGACCGT 
TCCAGCACTG TCAAAGACCT GATCTCCAGG CTGCTGCAGG TGGATCCTGA GGCACGCCTG 
ACAGCTGAGC AGGCCCTACA GCACCCCTTC TTTGAGCGTT GTGAAGGCAG CCAACCCTGG 
AACCTCACCC CCCGCCAGCG GTTCCGGGTG GCAGTGTGGA CAGTGCTGGC TGCTGGACGA 
GTGGCCCTAA GCACCCATCG TGTACGGCCA CTGACCAAGA ATGCACTGTT GAGGGACCCT 
TATGCGCTGC GGTCAGTGCG GCACCTCATC GACAACTGTG CCTTCCGGCT CTACGGGCAC 
TGGATAAGGA AGCAGTGGAT TGGAAAGCTG ATGGCTTGTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

Novel PHKG2 mutation causing GSD IX with prominent liver disease: report of three cases and review of literature.
European journal of pediatrics173(5)647-53(2014 May)
Albash B,Imtiaz F,Al-Zaidan H,Al-Manea H,Banemai M,Allam R,Al-Suheel A,Al-Owain M

Variability of disease spectrum in children with liver phosphorylase kinase deficiency caused by mutations in the PHKG2 gene.
Molecular genetics and metabolism111(3)309-313(2014 Mar)
Bali DS,Goldstein JL,Fredrickson K,Rehder C,Boney A,Austin S,Weinstein DA,Lutz R,Boneh A,Kishnani PS

Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS.
Nature methods10(4)307-14(2013 Apr)
Varjosalo M,Sacco R,Stukalov A,van Drogen A,Planyavsky M,Hauri S,Aebersold R,Bennett KL,Colinge J,Gstaiger M,Superti-Furga G

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PHKG2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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