PFKFB1 cDNA ORF clone, Homo sapiens(human)

The following PFKFB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFKFB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu20318 NM_002625.4
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu03896 NM_001271805.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu09773 NM_001271804.2
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu54096 XM_024452389.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu99334 XM_017029577.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu99335 XM_017029578.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu99333 XM_017029576.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu20318 NM_002625.3 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu09773 NM_001271804.1 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu20318
    Clone ID Related Accession (Same CDS sequence) NM_002625.4 , NM_002625.3
    Accession Version NM_002625.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-24
    Organism Homo sapiens(human)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL020991.1, X52638.1, AK314089.1, AL703826.1 and AL049732.11. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_002625.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189664.85930.1, X52638.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved MANE Ensembl match :: ENST00000375006.8/ ENSP00000364145.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
    AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT
    TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA
    AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGTGTTTAA TTTAGGCCAG
    TATCGACGAG AGGCAGTGAG CTACAAGAAC TATGAATTCT TTCTTCCAGA CAACATGGAA
    GCCCTGCAAA TCAGGAAGCA GTGCGCCCTG GCAGCCCTGA AGGATGTTCA CAACTATCTC
    AGCCATGAGG AAGGTCATGT TGCGGTTTTT GATGCCACCA ACACTACCAG AGAACGACGG
    TCACTGATCC TGCAGTTTGC AAAAGAACAT GGTTACAAGG TGTTTTTCAT TGAGTCCATT
    TGTAATGACC CTGGCATAAT TGCAGAAAAC ATCAGGCAAG TGAAACTTGG CAGCCCTGAT
    TATATAGACT GTGACCGGGA AAAGGTTCTG GAAGACTTTC TAAAGAGAAT TGAGTGCTAT
    GAGGTCAACT ACCAACCCTT GGATGAGGAA CTGGACAGCC ACCTGTCCTA CATCAAGATC
    TTCGACGTGG GCACACGCTA CATGGTGAAC CGAGTGCAGG ATCACATCCA GAGCCGCACA
    GTCTACTACC TCATGAATAT CCATGTCACA CCTCGCTCCA TCTACCTTTG CCGACATGGC
    GAGAGTGAAC TCAACATCAG AGGCCGCATC GGAGGTGACT CTGGCCTCTC AGTTCGCGGC
    AAGCAGTATG CCTATGCCCT GGCCAACTTC ATTCAGTCCC AGGGCATCAG CTCCCTGAAG
    GTGTGGACCA GTCACATGAA GAGGACCATC CAGACAGCTG AGGCCCTGGG TGTCCCCTAT
    GAGCAGTGGA AGGCCCTGAA TGAGATTGAT GCGGGTGTCT GTGAGGAGAT GACCTATGAA
    GAAATCCAGG AACATTACCC TGAAGAATTT GCACTGCGAG ACCAAGATAA ATATCGCTAC
    CGCTATCCCA AGGGAGAGTC CTATGAGGAT CTGGTTCAGC GTCTGGAGCC AGTGATAATG
    GAGCTAGAAC GACAGGAGAA TGTACTGGTG ATCTGCCACC AGGCTGTCAT GCGGTGCCTC
    CTGGCCTATT TCCTGGATAA AAGTTCAGAT GAGCTTCCAT ATCTCAAGTG CCCTCTGCAC
    ACAGTGCTCA AACTCACTCC TGTGGCTTAT GGCTGCAAAG TGGAATCCAT CTACCTGAAT
    GTGGAGGCCG TGAACACACA CCGGGAGAAG CCTGAGAATG TGGACATCAC CCGGGAACCT
    GAGGAAGCCC TGGATACTGT CCCAGCCCAC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002616.2
    CDS182..1597
    Translation

    Target ORF information:

    RefSeq Version NM_002625.4
    Organism Homo sapiens(human)
    Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002625.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
    AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT
    TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA
    AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGTGTTTAA TTTAGGCCAG
    TATCGACGAG AGGCAGTGAG CTACAAGAAC TATGAATTCT TTCTTCCAGA CAACATGGAA
    GCCCTGCAAA TCAGGAAGCA GTGCGCCCTG GCAGCCCTGA AGGATGTTCA CAACTATCTC
    AGCCATGAGG AAGGTCATGT TGCGGTTTTT GATGCCACCA ACACTACCAG AGAACGACGG
    TCACTGATCC TGCAGTTTGC AAAAGAACAT GGTTACAAGG TGTTTTTCAT TGAGTCCATT
    TGTAATGACC CTGGCATAAT TGCAGAAAAC ATCAGGCAAG TGAAACTTGG CAGCCCTGAT
    TATATAGACT GTGACCGGGA AAAGGTTCTG GAAGACTTTC TAAAGAGAAT TGAGTGCTAT
    GAGGTCAACT ACCAACCCTT GGATGAGGAA CTGGACAGCC ACCTGTCCTA CATCAAGATC
    TTCGACGTGG GCACACGCTA CATGGTGAAC CGAGTGCAGG ATCACATCCA GAGCCGCACA
    GTCTACTACC TCATGAATAT CCATGTCACA CCTCGCTCCA TCTACCTTTG CCGACATGGC
    GAGAGTGAAC TCAACATCAG AGGCCGCATC GGAGGTGACT CTGGCCTCTC AGTTCGCGGC
    AAGCAGTATG CCTATGCCCT GGCCAACTTC ATTCAGTCCC AGGGCATCAG CTCCCTGAAG
    GTGTGGACCA GTCACATGAA GAGGACCATC CAGACAGCTG AGGCCCTGGG TGTCCCCTAT
    GAGCAGTGGA AGGCCCTGAA TGAGATTGAT GCGGGTGTCT GTGAGGAGAT GACCTATGAA
    GAAATCCAGG AACATTACCC TGAAGAATTT GCACTGCGAG ACCAAGATAA ATATCGCTAC
    CGCTATCCCA AGGGAGAGTC CTATGAGGAT CTGGTTCAGC GTCTGGAGCC AGTGATAATG
    GAGCTAGAAC GACAGGAGAA TGTACTGGTG ATCTGCCACC AGGCTGTCAT GCGGTGCCTC
    CTGGCCTATT TCCTGGATAA AAGTTCAGAT GAGCTTCCAT ATCTCAAGTG CCCTCTGCAC
    ACAGTGCTCA AACTCACTCC TGTGGCTTAT GGCTGCAAAG TGGAATCCAT CTACCTGAAT
    GTGGAGGCCG TGAACACACA CCGGGAGAAG CCTGAGAATG TGGACATCAC CCGGGAACCT
    GAGGAAGCCC TGGATACTGT CCCAGCCCAC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20318
    Clone ID Related Accession (Same CDS sequence) NM_002625.4 , NM_002625.3
    Accession Version NM_002625.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X52638.1, AK314089.1 and AL703826.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2012 this sequence version replaced NM_002625.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X52638.1, BC096078.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
    AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT
    TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA
    AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGTGTTTAA TTTAGGCCAG
    TATCGACGAG AGGCAGTGAG CTACAAGAAC TATGAATTCT TTCTTCCAGA CAACATGGAA
    GCCCTGCAAA TCAGGAAGCA GTGCGCCCTG GCAGCCCTGA AGGATGTTCA CAACTATCTC
    AGCCATGAGG AAGGTCATGT TGCGGTTTTT GATGCCACCA ACACTACCAG AGAACGACGG
    TCACTGATCC TGCAGTTTGC AAAAGAACAT GGTTACAAGG TGTTTTTCAT TGAGTCCATT
    TGTAATGACC CTGGCATAAT TGCAGAAAAC ATCAGGCAAG TGAAACTTGG CAGCCCTGAT
    TATATAGACT GTGACCGGGA AAAGGTTCTG GAAGACTTTC TAAAGAGAAT TGAGTGCTAT
    GAGGTCAACT ACCAACCCTT GGATGAGGAA CTGGACAGCC ACCTGTCCTA CATCAAGATC
    TTCGACGTGG GCACACGCTA CATGGTGAAC CGAGTGCAGG ATCACATCCA GAGCCGCACA
    GTCTACTACC TCATGAATAT CCATGTCACA CCTCGCTCCA TCTACCTTTG CCGACATGGC
    GAGAGTGAAC TCAACATCAG AGGCCGCATC GGAGGTGACT CTGGCCTCTC AGTTCGCGGC
    AAGCAGTATG CCTATGCCCT GGCCAACTTC ATTCAGTCCC AGGGCATCAG CTCCCTGAAG
    GTGTGGACCA GTCACATGAA GAGGACCATC CAGACAGCTG AGGCCCTGGG TGTCCCCTAT
    GAGCAGTGGA AGGCCCTGAA TGAGATTGAT GCGGGTGTCT GTGAGGAGAT GACCTATGAA
    GAAATCCAGG AACATTACCC TGAAGAATTT GCACTGCGAG ACCAAGATAA ATATCGCTAC
    CGCTATCCCA AGGGAGAGTC CTATGAGGAT CTGGTTCAGC GTCTGGAGCC AGTGATAATG
    GAGCTAGAAC GACAGGAGAA TGTACTGGTG ATCTGCCACC AGGCTGTCAT GCGGTGCCTC
    CTGGCCTATT TCCTGGATAA AAGTTCAGAT GAGCTTCCAT ATCTCAAGTG CCCTCTGCAC
    ACAGTGCTCA AACTCACTCC TGTGGCTTAT GGCTGCAAAG TGGAATCCAT CTACCTGAAT
    GTGGAGGCCG TGAACACACA CCGGGAGAAG CCTGAGAATG TGGACATCAC CCGGGAACCT
    GAGGAAGCCC TGGATACTGT CCCAGCCCAC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002616.2
    CDS72..1487
    Translation

    Target ORF information:

    RefSeq Version NM_002625.3
    Organism Homo sapiens(human)
    Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002625.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
    AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT
    TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA
    AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGTGTTTAA TTTAGGCCAG
    TATCGACGAG AGGCAGTGAG CTACAAGAAC TATGAATTCT TTCTTCCAGA CAACATGGAA
    GCCCTGCAAA TCAGGAAGCA GTGCGCCCTG GCAGCCCTGA AGGATGTTCA CAACTATCTC
    AGCCATGAGG AAGGTCATGT TGCGGTTTTT GATGCCACCA ACACTACCAG AGAACGACGG
    TCACTGATCC TGCAGTTTGC AAAAGAACAT GGTTACAAGG TGTTTTTCAT TGAGTCCATT
    TGTAATGACC CTGGCATAAT TGCAGAAAAC ATCAGGCAAG TGAAACTTGG CAGCCCTGAT
    TATATAGACT GTGACCGGGA AAAGGTTCTG GAAGACTTTC TAAAGAGAAT TGAGTGCTAT
    GAGGTCAACT ACCAACCCTT GGATGAGGAA CTGGACAGCC ACCTGTCCTA CATCAAGATC
    TTCGACGTGG GCACACGCTA CATGGTGAAC CGAGTGCAGG ATCACATCCA GAGCCGCACA
    GTCTACTACC TCATGAATAT CCATGTCACA CCTCGCTCCA TCTACCTTTG CCGACATGGC
    GAGAGTGAAC TCAACATCAG AGGCCGCATC GGAGGTGACT CTGGCCTCTC AGTTCGCGGC
    AAGCAGTATG CCTATGCCCT GGCCAACTTC ATTCAGTCCC AGGGCATCAG CTCCCTGAAG
    GTGTGGACCA GTCACATGAA GAGGACCATC CAGACAGCTG AGGCCCTGGG TGTCCCCTAT
    GAGCAGTGGA AGGCCCTGAA TGAGATTGAT GCGGGTGTCT GTGAGGAGAT GACCTATGAA
    GAAATCCAGG AACATTACCC TGAAGAATTT GCACTGCGAG ACCAAGATAA ATATCGCTAC
    CGCTATCCCA AGGGAGAGTC CTATGAGGAT CTGGTTCAGC GTCTGGAGCC AGTGATAATG
    GAGCTAGAAC GACAGGAGAA TGTACTGGTG ATCTGCCACC AGGCTGTCAT GCGGTGCCTC
    CTGGCCTATT TCCTGGATAA AAGTTCAGAT GAGCTTCCAT ATCTCAAGTG CCCTCTGCAC
    ACAGTGCTCA AACTCACTCC TGTGGCTTAT GGCTGCAAAG TGGAATCCAT CTACCTGAAT
    GTGGAGGCCG TGAACACACA CCGGGAGAAG CCTGAGAATG TGGACATCAC CCGGGAACCT
    GAGGAAGCCC TGGATACTGT CCCAGCCCAC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu03896
    Clone ID Related Accession (Same CDS sequence) NM_001271805.1
    Accession Version NM_001271805.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-05
    Organism Homo sapiens(human)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300724.1. Transcript Variant: This variant (3) lacks two 5' exons and contains an alternate 5' exon, compared to variant 1. The resulting isoform (3) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300724.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGAAGAAA AAACCTCTAG AATAAAAGTG TTTAATTTAG GCCAGTATCG ACGAGAGGCA 
    GTGAGCTACA AGAACTATGA ATTCTTTCTT CCAGACAACA TGGAAGCCCT GCAAATCAGG
    AAGCAGTGCG CCCTGGCAGC CCTGAAGGAT GTTCACAACT ATCTCAGCCA TGAGGAAGGT
    CATGTTGCGG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATCCTGCAG
    TTTGCAAAAG AACATGGTTA CAAGGTGTTT TTCATTGAGT CCATTTGTAA TGACCCTGGC
    ATAATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGCC CTGATTATAT AGACTGTGAC
    CGGGAAAAGG TTCTGGAAGA CTTTCTAAAG AGAATTGAGT GCTATGAGGT CAACTACCAA
    CCCTTGGATG AGGAACTGGA CAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA
    CGCTACATGG TGAACCGAGT GCAGGATCAC ATCCAGAGCC GCACAGTCTA CTACCTCATG
    AATATCCATG TCACACCTCG CTCCATCTAC CTTTGCCGAC ATGGCGAGAG TGAACTCAAC
    ATCAGAGGCC GCATCGGAGG TGACTCTGGC CTCTCAGTTC GCGGCAAGCA GTATGCCTAT
    GCCCTGGCCA ACTTCATTCA GTCCCAGGGC ATCAGCTCCC TGAAGGTGTG GACCAGTCAC
    ATGAAGAGGA CCATCCAGAC AGCTGAGGCC CTGGGTGTCC CCTATGAGCA GTGGAAGGCC
    CTGAATGAGA TTGATGCGGG TGTCTGTGAG GAGATGACCT ATGAAGAAAT CCAGGAACAT
    TACCCTGAAG AATTTGCACT GCGAGACCAA GATAAATATC GCTACCGCTA TCCCAAGGGA
    GAGTCCTATG AGGATCTGGT TCAGCGTCTG GAGCCAGTGA TAATGGAGCT AGAACGACAG
    GAGAATGTAC TGGTGATCTG CCACCAGGCT GTCATGCGGT GCCTCCTGGC CTATTTCCTG
    GATAAAAGTT CAGATGAGCT TCCATATCTC AAGTGCCCTC TGCACACAGT GCTCAAACTC
    ACTCCTGTGG CTTATGGCTG CAAAGTGGAA TCCATCTACC TGAATGTGGA GGCCGTGAAC
    ACACACCGGG AGAAGCCTGA GAATGTGGAC ATCACCCGGG AACCTGAGGA AGCCCTGGAT
    ACTGTCCCAG CCCACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258734.1
    CDS102..1322
    Translation

    Target ORF information:

    RefSeq Version NM_001271805.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271805.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGAAGAAA AAACCTCTAG AATAAAAGTG TTTAATTTAG GCCAGTATCG ACGAGAGGCA 
    GTGAGCTACA AGAACTATGA ATTCTTTCTT CCAGACAACA TGGAAGCCCT GCAAATCAGG
    AAGCAGTGCG CCCTGGCAGC CCTGAAGGAT GTTCACAACT ATCTCAGCCA TGAGGAAGGT
    CATGTTGCGG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATCCTGCAG
    TTTGCAAAAG AACATGGTTA CAAGGTGTTT TTCATTGAGT CCATTTGTAA TGACCCTGGC
    ATAATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGCC CTGATTATAT AGACTGTGAC
    CGGGAAAAGG TTCTGGAAGA CTTTCTAAAG AGAATTGAGT GCTATGAGGT CAACTACCAA
    CCCTTGGATG AGGAACTGGA CAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA
    CGCTACATGG TGAACCGAGT GCAGGATCAC ATCCAGAGCC GCACAGTCTA CTACCTCATG
    AATATCCATG TCACACCTCG CTCCATCTAC CTTTGCCGAC ATGGCGAGAG TGAACTCAAC
    ATCAGAGGCC GCATCGGAGG TGACTCTGGC CTCTCAGTTC GCGGCAAGCA GTATGCCTAT
    GCCCTGGCCA ACTTCATTCA GTCCCAGGGC ATCAGCTCCC TGAAGGTGTG GACCAGTCAC
    ATGAAGAGGA CCATCCAGAC AGCTGAGGCC CTGGGTGTCC CCTATGAGCA GTGGAAGGCC
    CTGAATGAGA TTGATGCGGG TGTCTGTGAG GAGATGACCT ATGAAGAAAT CCAGGAACAT
    TACCCTGAAG AATTTGCACT GCGAGACCAA GATAAATATC GCTACCGCTA TCCCAAGGGA
    GAGTCCTATG AGGATCTGGT TCAGCGTCTG GAGCCAGTGA TAATGGAGCT AGAACGACAG
    GAGAATGTAC TGGTGATCTG CCACCAGGCT GTCATGCGGT GCCTCCTGGC CTATTTCCTG
    GATAAAAGTT CAGATGAGCT TCCATATCTC AAGTGCCCTC TGCACACAGT GCTCAAACTC
    ACTCCTGTGG CTTATGGCTG CAAAGTGGAA TCCATCTACC TGAATGTGGA GGCCGTGAAC
    ACACACCGGG AGAAGCCTGA GAATGTGGAC ATCACCCGGG AACCTGAGGA AGCCCTGGAT
    ACTGTCCCAG CCCACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09773
    Clone ID Related Accession (Same CDS sequence) NM_001271804.2 , NM_001271804.1
    Accession Version NM_001271804.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-06
    Organism Homo sapiens(human)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL020991.1, AK314089.1, JQ425695.1, AK300724.1 and AL049732.11. On May 31, 2019 this sequence version replaced NM_001271804.1. Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JQ425695.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
    AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT
    TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA
    AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGACAACAT GGAAGCCCTG
    CAAATCAGGA AGCAGTGCGC CCTGGCAGCC CTGAAGGATG TTCACAACTA TCTCAGCCAT
    GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGGTCACTG
    ATCCTGCAGT TTGCAAAAGA ACATGGTTAC AAGGTGTTTT TCATTGAGTC CATTTGTAAT
    GACCCTGGCA TAATTGCAGA AAACATCAGG CAAGTGAAAC TTGGCAGCCC TGATTATATA
    GACTGTGACC GGGAAAAGGT TCTGGAAGAC TTTCTAAAGA GAATTGAGTG CTATGAGGTC
    AACTACCAAC CCTTGGATGA GGAACTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC
    GTGGGCACAC GCTACATGGT GAACCGAGTG CAGGATCACA TCCAGAGCCG CACAGTCTAC
    TACCTCATGA ATATCCATGT CACACCTCGC TCCATCTACC TTTGCCGACA TGGCGAGAGT
    GAACTCAACA TCAGAGGCCG CATCGGAGGT GACTCTGGCC TCTCAGTTCG CGGCAAGCAG
    TATGCCTATG CCCTGGCCAA CTTCATTCAG TCCCAGGGCA TCAGCTCCCT GAAGGTGTGG
    ACCAGTCACA TGAAGAGGAC CATCCAGACA GCTGAGGCCC TGGGTGTCCC CTATGAGCAG
    TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAGG AGATGACCTA TGAAGAAATC
    CAGGAACATT ACCCTGAAGA ATTTGCACTG CGAGACCAAG ATAAATATCG CTACCGCTAT
    CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTGG AGCCAGTGAT AATGGAGCTA
    GAACGACAGG AGAATGTACT GGTGATCTGC CACCAGGCTG TCATGCGGTG CCTCCTGGCC
    TATTTCCTGG ATAAAAGTTC AGATGAGCTT CCATATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTCA CTCCTGTGGC TTATGGCTGC AAAGTGGAAT CCATCTACCT GAATGTGGAG
    GCCGTGAACA CACACCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAGGAA
    GCCCTGGATA CTGTCCCAGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258733.1
    CDS182..1531
    Translation

    Target ORF information:

    RefSeq Version NM_001271804.2
    Organism Homo sapiens(human)
    Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271804.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
    AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT
    TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA
    AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGACAACAT GGAAGCCCTG
    CAAATCAGGA AGCAGTGCGC CCTGGCAGCC CTGAAGGATG TTCACAACTA TCTCAGCCAT
    GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGGTCACTG
    ATCCTGCAGT TTGCAAAAGA ACATGGTTAC AAGGTGTTTT TCATTGAGTC CATTTGTAAT
    GACCCTGGCA TAATTGCAGA AAACATCAGG CAAGTGAAAC TTGGCAGCCC TGATTATATA
    GACTGTGACC GGGAAAAGGT TCTGGAAGAC TTTCTAAAGA GAATTGAGTG CTATGAGGTC
    AACTACCAAC CCTTGGATGA GGAACTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC
    GTGGGCACAC GCTACATGGT GAACCGAGTG CAGGATCACA TCCAGAGCCG CACAGTCTAC
    TACCTCATGA ATATCCATGT CACACCTCGC TCCATCTACC TTTGCCGACA TGGCGAGAGT
    GAACTCAACA TCAGAGGCCG CATCGGAGGT GACTCTGGCC TCTCAGTTCG CGGCAAGCAG
    TATGCCTATG CCCTGGCCAA CTTCATTCAG TCCCAGGGCA TCAGCTCCCT GAAGGTGTGG
    ACCAGTCACA TGAAGAGGAC CATCCAGACA GCTGAGGCCC TGGGTGTCCC CTATGAGCAG
    TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAGG AGATGACCTA TGAAGAAATC
    CAGGAACATT ACCCTGAAGA ATTTGCACTG CGAGACCAAG ATAAATATCG CTACCGCTAT
    CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTGG AGCCAGTGAT AATGGAGCTA
    GAACGACAGG AGAATGTACT GGTGATCTGC CACCAGGCTG TCATGCGGTG CCTCCTGGCC
    TATTTCCTGG ATAAAAGTTC AGATGAGCTT CCATATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTCA CTCCTGTGGC TTATGGCTGC AAAGTGGAAT CCATCTACCT GAATGTGGAG
    GCCGTGAACA CACACCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAGGAA
    GCCCTGGATA CTGTCCCAGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09773
    Clone ID Related Accession (Same CDS sequence) NM_001271804.2 , NM_001271804.1
    Accession Version NM_001271804.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-24
    Organism Homo sapiens(human)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314089.1, JQ425695.1 and AK300724.1. Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JQ425695.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
    AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT
    TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA
    AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGACAACAT GGAAGCCCTG
    CAAATCAGGA AGCAGTGCGC CCTGGCAGCC CTGAAGGATG TTCACAACTA TCTCAGCCAT
    GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGGTCACTG
    ATCCTGCAGT TTGCAAAAGA ACATGGTTAC AAGGTGTTTT TCATTGAGTC CATTTGTAAT
    GACCCTGGCA TAATTGCAGA AAACATCAGG CAAGTGAAAC TTGGCAGCCC TGATTATATA
    GACTGTGACC GGGAAAAGGT TCTGGAAGAC TTTCTAAAGA GAATTGAGTG CTATGAGGTC
    AACTACCAAC CCTTGGATGA GGAACTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC
    GTGGGCACAC GCTACATGGT GAACCGAGTG CAGGATCACA TCCAGAGCCG CACAGTCTAC
    TACCTCATGA ATATCCATGT CACACCTCGC TCCATCTACC TTTGCCGACA TGGCGAGAGT
    GAACTCAACA TCAGAGGCCG CATCGGAGGT GACTCTGGCC TCTCAGTTCG CGGCAAGCAG
    TATGCCTATG CCCTGGCCAA CTTCATTCAG TCCCAGGGCA TCAGCTCCCT GAAGGTGTGG
    ACCAGTCACA TGAAGAGGAC CATCCAGACA GCTGAGGCCC TGGGTGTCCC CTATGAGCAG
    TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAGG AGATGACCTA TGAAGAAATC
    CAGGAACATT ACCCTGAAGA ATTTGCACTG CGAGACCAAG ATAAATATCG CTACCGCTAT
    CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTGG AGCCAGTGAT AATGGAGCTA
    GAACGACAGG AGAATGTACT GGTGATCTGC CACCAGGCTG TCATGCGGTG CCTCCTGGCC
    TATTTCCTGG ATAAAAGTTC AGATGAGCTT CCATATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTCA CTCCTGTGGC TTATGGCTGC AAAGTGGAAT CCATCTACCT GAATGTGGAG
    GCCGTGAACA CACACCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAGGAA
    GCCCTGGATA CTGTCCCAGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258733.1
    CDS72..1421
    Translation

    Target ORF information:

    RefSeq Version NM_001271804.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271804.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
    AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT
    TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA
    AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGACAACAT GGAAGCCCTG
    CAAATCAGGA AGCAGTGCGC CCTGGCAGCC CTGAAGGATG TTCACAACTA TCTCAGCCAT
    GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGGTCACTG
    ATCCTGCAGT TTGCAAAAGA ACATGGTTAC AAGGTGTTTT TCATTGAGTC CATTTGTAAT
    GACCCTGGCA TAATTGCAGA AAACATCAGG CAAGTGAAAC TTGGCAGCCC TGATTATATA
    GACTGTGACC GGGAAAAGGT TCTGGAAGAC TTTCTAAAGA GAATTGAGTG CTATGAGGTC
    AACTACCAAC CCTTGGATGA GGAACTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC
    GTGGGCACAC GCTACATGGT GAACCGAGTG CAGGATCACA TCCAGAGCCG CACAGTCTAC
    TACCTCATGA ATATCCATGT CACACCTCGC TCCATCTACC TTTGCCGACA TGGCGAGAGT
    GAACTCAACA TCAGAGGCCG CATCGGAGGT GACTCTGGCC TCTCAGTTCG CGGCAAGCAG
    TATGCCTATG CCCTGGCCAA CTTCATTCAG TCCCAGGGCA TCAGCTCCCT GAAGGTGTGG
    ACCAGTCACA TGAAGAGGAC CATCCAGACA GCTGAGGCCC TGGGTGTCCC CTATGAGCAG
    TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAGG AGATGACCTA TGAAGAAATC
    CAGGAACATT ACCCTGAAGA ATTTGCACTG CGAGACCAAG ATAAATATCG CTACCGCTAT
    CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTGG AGCCAGTGAT AATGGAGCTA
    GAACGACAGG AGAATGTACT GGTGATCTGC CACCAGGCTG TCATGCGGTG CCTCCTGGCC
    TATTTCCTGG ATAAAAGTTC AGATGAGCTT CCATATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTCA CTCCTGTGGC TTATGGCTGC AAAGTGGAAT CCATCTACCT GAATGTGGAG
    GCCGTGAACA CACACCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAGGAA
    GCCCTGGATA CTGTCCCAGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu54096
    Clone ID Related Accession (Same CDS sequence) XM_024452389.1
    Accession Version XM_024452389.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAAGAAA AAACCTCTAG AATAAAAGCA TCCATACCCC AGTTTACCAA TTCCCCCACA 
    ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGCAAGACCT ATATCTCCAC AAAGCTCACA
    CGATATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGCCA GTATCGACGA
    GAGGCAGTGA GCTACAAGAA CTATGAATTC TTTCTTCCAG ACAACATGGA AGCCCTGCAA
    ATCAGGAAGC AGTGCGCCCT GGCAGCCCTG AAGGATGTTC ACAACTATCT CAGCCATGAG
    GAAGGTCATG TTGCGGTTTT TGATGCCACC AACACTACCA GAGAACGACG GTCACTGATC
    CTGCAGTTTG CAAAAGAACA TGGTTACAAG GTGTTTTTCA TTGAGTCCAT TTGTAATGAC
    CCTGGCATAA TTGCAGAAAA CATCAGGCAA GTGAAACTTG GCAGCCCTGA TTATATAGAC
    TGTGACCGGG AAAAGGTTCT GGAAGACTTT CTAAAGAGAA TTGAGTGCTA TGAGGTCAAC
    TACCAACCCT TGGATGAGGA ACTGGACAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG
    GGCACACGCT ACATGGTGAA CCGAGTGCAG GATCACATCC AGAGCCGCAC AGTCTACTAC
    CTCATGAATA TCCATGTCAC ACCTCGCTCC ATCTACCTTT GCCGACATGG CGAGAGTGAA
    CTCAACATCA GAGGCCGCAT CGGAGGTGAC TCTGGCCTCT CAGTTCGCGG CAAGCAGTAT
    GCCTATGCCC TGGCCAACTT CATTCAGTCC CAGGGCATCA GCTCCCTGAA GGTGTGGACC
    AGTCACATGA AGAGGACCAT CCAGACAGCT GAGGCCCTGG GTGTCCCCTA TGAGCAGTGG
    AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAGGAGA TGACCTATGA AGAAATCCAG
    GAACATTACC CTGAAGAATT TGCACTGCGA GACCAAGATA AATATCGCTA CCGCTATCCC
    AAGGGAGAGT CCTATGAGGA TCTGGTTCAG CGTCTGGAGC CAGTGATAAT GGAGCTAGAA
    CGACAGGAGA ATGTACTGGT GATCTGCCAC CAGGCTGTCA TGCGGTGCCT CCTGGCCTAT
    TTCCTGGATA AAAGTTCAGA TGAGCTTCCA TATCTCAAGT GCCCTCTGCA CACAGTGCTC
    AAACTCACTC CTGTGGCTTA TGGCTGCAAA GTGGAATCCA TCTACCTGAA TGTGGAGGCC
    GTGAACACAC ACCGGGAGAA GCCTGAGAAT GTGGACATCA CCCGGGAACC TGAGGAAGCC
    CTGGATACTG TCCCAGCCCA CTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308157.1
    CDS1620..2966
    Translation

    Target ORF information:

    RefSeq Version XM_024452389.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452389.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAAGAAA AAACCTCTAG AATAAAAGCA TCCATACCCC AGTTTACCAA TTCCCCCACA 
    ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGCAAGACCT ATATCTCCAC AAAGCTCACA
    CGATATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGCCA GTATCGACGA
    GAGGCAGTGA GCTACAAGAA CTATGAATTC TTTCTTCCAG ACAACATGGA AGCCCTGCAA
    ATCAGGAAGC AGTGCGCCCT GGCAGCCCTG AAGGATGTTC ACAACTATCT CAGCCATGAG
    GAAGGTCATG TTGCGGTTTT TGATGCCACC AACACTACCA GAGAACGACG GTCACTGATC
    CTGCAGTTTG CAAAAGAACA TGGTTACAAG GTGTTTTTCA TTGAGTCCAT TTGTAATGAC
    CCTGGCATAA TTGCAGAAAA CATCAGGCAA GTGAAACTTG GCAGCCCTGA TTATATAGAC
    TGTGACCGGG AAAAGGTTCT GGAAGACTTT CTAAAGAGAA TTGAGTGCTA TGAGGTCAAC
    TACCAACCCT TGGATGAGGA ACTGGACAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG
    GGCACACGCT ACATGGTGAA CCGAGTGCAG GATCACATCC AGAGCCGCAC AGTCTACTAC
    CTCATGAATA TCCATGTCAC ACCTCGCTCC ATCTACCTTT GCCGACATGG CGAGAGTGAA
    CTCAACATCA GAGGCCGCAT CGGAGGTGAC TCTGGCCTCT CAGTTCGCGG CAAGCAGTAT
    GCCTATGCCC TGGCCAACTT CATTCAGTCC CAGGGCATCA GCTCCCTGAA GGTGTGGACC
    AGTCACATGA AGAGGACCAT CCAGACAGCT GAGGCCCTGG GTGTCCCCTA TGAGCAGTGG
    AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAGGAGA TGACCTATGA AGAAATCCAG
    GAACATTACC CTGAAGAATT TGCACTGCGA GACCAAGATA AATATCGCTA CCGCTATCCC
    AAGGGAGAGT CCTATGAGGA TCTGGTTCAG CGTCTGGAGC CAGTGATAAT GGAGCTAGAA
    CGACAGGAGA ATGTACTGGT GATCTGCCAC CAGGCTGTCA TGCGGTGCCT CCTGGCCTAT
    TTCCTGGATA AAAGTTCAGA TGAGCTTCCA TATCTCAAGT GCCCTCTGCA CACAGTGCTC
    AAACTCACTC CTGTGGCTTA TGGCTGCAAA GTGGAATCCA TCTACCTGAA TGTGGAGGCC
    GTGAACACAC ACCGGGAGAA GCCTGAGAAT GTGGACATCA CCCGGGAACC TGAGGAAGCC
    CTGGATACTG TCCCAGCCCA CTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99334
    Clone ID Related Accession (Same CDS sequence) XM_017029577.1
    Accession Version XM_017029577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCATTCG GTGCGACTCC TAAGATGTCT CCAGAGATGG GAGAGCTCAC CCAAACCAGG 
    TTGCAGAAGA TCTGGATTCC ACACAGCAGC GGCAGCAGCA GGCTGCAACG GAGAAGGGGC
    TCATCCATAC CCCAGTTTAC CAATTCCCCC ACAATGGTGA TCATGGTGGG TTTACCAGCT
    CGAGGCAAGA CCTATATCTC CACAAAGCTC ACACGATATC TCAACTGGAT AGGAACACCA
    ACTAAAGCAA GGACATTTTT TTGGCCACTG GGATCTTCCC AACCTCTTCA CTGGCACTCC
    CTGCTTTCAG TGTTTAATTT AGGCCAGTAT CGACGAGAGG CAGTGAGCTA CAAGAACTAT
    GAATTCTTTC TTCCAGACAA CATGGAAGCC CTGCAAATCA GGAAGCAGTG CGCCCTGGCA
    GCCCTGAAGG ATGTTCACAA CTATCTCAGC CATGAGGAAG GTCATGTTGC GGTTTTTGAT
    GCCACCAACA CTACCAGAGA ACGACGGTCA CTGATCCTGC AGTTTGCAAA AGAACATGGT
    TACAAGGTGT TTTTCATTGA GTCCATTTGT AATGACCCTG GCATAATTGC AGAAAACATC
    AGGCAAGTGA AACTTGGCAG CCCTGATTAT ATAGACTGTG ACCGGGAAAA GGTTCTGGAA
    GACTTTCTAA AGAGAATTGA GTGCTATGAG GTCAACTACC AACCCTTGGA TGAGGAACTG
    GACAGCCACC TGTCCTACAT CAAGATCTTC GACGTGGGCA CACGCTACAT GGTGAACCGA
    GTGCAGGATC ACATCCAGAG CCGCACAGTC TACTACCTCA TGAATATCCA TGTCACACCT
    CGCTCCATCT ACCTTTGCCG ACATGGCGAG AGTGAACTCA ACATCAGAGG CCGCATCGGA
    GGTGACTCTG GCCTCTCAGT TCGCGGCAAG CAGTATGCCT ATGCCCTGGC CAACTTCATT
    CAGTCCCAGG GCATCAGCTC CCTGAAGGTG TGGACCAGTC ACATGAAGAG GACCATCCAG
    ACAGCTGAGG CCCTGGGTGT CCCCTATGAG CAGTGGAAGG CCCTGAATGA GATTGATGCG
    GGTGTCTGTG AGGAGATGAC CTATGAAGAA ATCCAGGAAC ATTACCCTGA AGAATTTGCA
    CTGCGAGACC AAGATAAATA TCGCTACCGC TATCCCAAGG GAGAGTCCTA TGAGGATCTG
    GTTCAGCGTC TGGAGCCAGT GATAATGGAG CTAGAACGAC AGGAGAATGT ACTGGTGATC
    TGCCACCAGG CTGTCATGCG GTGCCTCCTG GCCTATTTCC TGGATAAAAG TTCAGGTGAA
    TGCTGGAACA AGGCCCAGAG AGATGATGTG ACTGAGAATG CAGACCCACA GAACATCAGG
    GCTGGGGCAC CCTCAGAGTA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885066.1
    CDS389..1795
    Translation

    Target ORF information:

    RefSeq Version XM_017029577.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCATTCG GTGCGACTCC TAAGATGTCT CCAGAGATGG GAGAGCTCAC CCAAACCAGG 
    TTGCAGAAGA TCTGGATTCC ACACAGCAGC GGCAGCAGCA GGCTGCAACG GAGAAGGGGC
    TCATCCATAC CCCAGTTTAC CAATTCCCCC ACAATGGTGA TCATGGTGGG TTTACCAGCT
    CGAGGCAAGA CCTATATCTC CACAAAGCTC ACACGATATC TCAACTGGAT AGGAACACCA
    ACTAAAGCAA GGACATTTTT TTGGCCACTG GGATCTTCCC AACCTCTTCA CTGGCACTCC
    CTGCTTTCAG TGTTTAATTT AGGCCAGTAT CGACGAGAGG CAGTGAGCTA CAAGAACTAT
    GAATTCTTTC TTCCAGACAA CATGGAAGCC CTGCAAATCA GGAAGCAGTG CGCCCTGGCA
    GCCCTGAAGG ATGTTCACAA CTATCTCAGC CATGAGGAAG GTCATGTTGC GGTTTTTGAT
    GCCACCAACA CTACCAGAGA ACGACGGTCA CTGATCCTGC AGTTTGCAAA AGAACATGGT
    TACAAGGTGT TTTTCATTGA GTCCATTTGT AATGACCCTG GCATAATTGC AGAAAACATC
    AGGCAAGTGA AACTTGGCAG CCCTGATTAT ATAGACTGTG ACCGGGAAAA GGTTCTGGAA
    GACTTTCTAA AGAGAATTGA GTGCTATGAG GTCAACTACC AACCCTTGGA TGAGGAACTG
    GACAGCCACC TGTCCTACAT CAAGATCTTC GACGTGGGCA CACGCTACAT GGTGAACCGA
    GTGCAGGATC ACATCCAGAG CCGCACAGTC TACTACCTCA TGAATATCCA TGTCACACCT
    CGCTCCATCT ACCTTTGCCG ACATGGCGAG AGTGAACTCA ACATCAGAGG CCGCATCGGA
    GGTGACTCTG GCCTCTCAGT TCGCGGCAAG CAGTATGCCT ATGCCCTGGC CAACTTCATT
    CAGTCCCAGG GCATCAGCTC CCTGAAGGTG TGGACCAGTC ACATGAAGAG GACCATCCAG
    ACAGCTGAGG CCCTGGGTGT CCCCTATGAG CAGTGGAAGG CCCTGAATGA GATTGATGCG
    GGTGTCTGTG AGGAGATGAC CTATGAAGAA ATCCAGGAAC ATTACCCTGA AGAATTTGCA
    CTGCGAGACC AAGATAAATA TCGCTACCGC TATCCCAAGG GAGAGTCCTA TGAGGATCTG
    GTTCAGCGTC TGGAGCCAGT GATAATGGAG CTAGAACGAC AGGAGAATGT ACTGGTGATC
    TGCCACCAGG CTGTCATGCG GTGCCTCCTG GCCTATTTCC TGGATAAAAG TTCAGGTGAA
    TGCTGGAACA AGGCCCAGAG AGATGATGTG ACTGAGAATG CAGACCCACA GAACATCAGG
    GCTGGGGCAC CCTCAGAGTA CATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99335
    Clone ID Related Accession (Same CDS sequence) XM_017029578.1
    Accession Version XM_017029578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGCAAGACCT ATATCTCCAC AAAGCTCACA 
    CGATATCTCA ACTGGATAGG AACACCAACT AAAGCAAGGA CATTTTTTTG GCCACTGGGA
    TCTTCCCAAC CTCTTCACTG GCACTCCCTG CTTTCAGTGT TTAATTTAGG CCAGTATCGA
    CGAGAGGCAG TGAGCTACAA GAACTATGAA TTCTTTCTTC CAGACAACAT GGAAGCCCTG
    CAAATCAGGA AGCAGTGCGC CCTGGCAGCC CTGAAGGATG TTCACAACTA TCTCAGCCAT
    GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGGTCACTG
    ATCCTGCAGT TTGCAAAAGA ACATGGTTAC AAGGTGTTTT TCATTGAGTC CATTTGTAAT
    GACCCTGGCA TAATTGCAGA AAACATCAGG CAAGTGAAAC TTGGCAGCCC TGATTATATA
    GACTGTGACC GGGAAAAGGT TCTGGAAGAC TTTCTAAAGA GAATTGAGTG CTATGAGGTC
    AACTACCAAC CCTTGGATGA GGAACTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC
    GTGGGCACAC GCTACATGGT GAACCGAGTG CAGGATCACA TCCAGAGCCG CACAGTCTAC
    TACCTCATGA ATATCCATGT CACACCTCGC TCCATCTACC TTTGCCGACA TGGCGAGAGT
    GAACTCAACA TCAGAGGCCG CATCGGAGGT GACTCTGGCC TCTCAGTTCG CGGCAAGCAG
    TATGCCTATG CCCTGGCCAA CTTCATTCAG TCCCAGGGCA TCAGCTCCCT GAAGGTGTGG
    ACCAGTCACA TGAAGAGGAC CATCCAGACA GCTGAGGCCC TGGGTGTCCC CTATGAGCAG
    TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAGG AGATGACCTA TGAAGAAATC
    CAGGAACATT ACCCTGAAGA ATTTGCACTG CGAGACCAAG ATAAATATCG CTACCGCTAT
    CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTGG AGCCAGTGAT AATGGAGCTA
    GAACGACAGG AGAATGTACT GGTGATCTGC CACCAGGCTG TCATGCGGTG CCTCCTGGCC
    TATTTCCTGG ATAAAAGTTC AGATGAGCTT CCATATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTCA CTCCTGTGGC TTATGGCTGC AAAGTGGAAT CCATCTACCT GAATGTGGAG
    GCCGTGAACA CACACCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAGGAA
    GCCCTGGATA CTGTCCCAGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885067.1
    CDS624..1973
    Translation

    Target ORF information:

    RefSeq Version XM_017029578.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGCAAGACCT ATATCTCCAC AAAGCTCACA 
    CGATATCTCA ACTGGATAGG AACACCAACT AAAGCAAGGA CATTTTTTTG GCCACTGGGA
    TCTTCCCAAC CTCTTCACTG GCACTCCCTG CTTTCAGTGT TTAATTTAGG CCAGTATCGA
    CGAGAGGCAG TGAGCTACAA GAACTATGAA TTCTTTCTTC CAGACAACAT GGAAGCCCTG
    CAAATCAGGA AGCAGTGCGC CCTGGCAGCC CTGAAGGATG TTCACAACTA TCTCAGCCAT
    GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGGTCACTG
    ATCCTGCAGT TTGCAAAAGA ACATGGTTAC AAGGTGTTTT TCATTGAGTC CATTTGTAAT
    GACCCTGGCA TAATTGCAGA AAACATCAGG CAAGTGAAAC TTGGCAGCCC TGATTATATA
    GACTGTGACC GGGAAAAGGT TCTGGAAGAC TTTCTAAAGA GAATTGAGTG CTATGAGGTC
    AACTACCAAC CCTTGGATGA GGAACTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC
    GTGGGCACAC GCTACATGGT GAACCGAGTG CAGGATCACA TCCAGAGCCG CACAGTCTAC
    TACCTCATGA ATATCCATGT CACACCTCGC TCCATCTACC TTTGCCGACA TGGCGAGAGT
    GAACTCAACA TCAGAGGCCG CATCGGAGGT GACTCTGGCC TCTCAGTTCG CGGCAAGCAG
    TATGCCTATG CCCTGGCCAA CTTCATTCAG TCCCAGGGCA TCAGCTCCCT GAAGGTGTGG
    ACCAGTCACA TGAAGAGGAC CATCCAGACA GCTGAGGCCC TGGGTGTCCC CTATGAGCAG
    TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAGG AGATGACCTA TGAAGAAATC
    CAGGAACATT ACCCTGAAGA ATTTGCACTG CGAGACCAAG ATAAATATCG CTACCGCTAT
    CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTGG AGCCAGTGAT AATGGAGCTA
    GAACGACAGG AGAATGTACT GGTGATCTGC CACCAGGCTG TCATGCGGTG CCTCCTGGCC
    TATTTCCTGG ATAAAAGTTC AGATGAGCTT CCATATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTCA CTCCTGTGGC TTATGGCTGC AAAGTGGAAT CCATCTACCT GAATGTGGAG
    GCCGTGAACA CACACCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAGGAA
    GCCCTGGATA CTGTCCCAGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99333
    Clone ID Related Accession (Same CDS sequence) XM_017029576.1
    Accession Version XM_017029576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCATTCG GTGCGACTCC TAAGATGTCT CCAGAGATGG GAGAGCTCAC CCAAACCAGG 
    TTGCAGAAGA TCTGGATTCC ACACAGCAGC GGCAGCAGCA GGCTGCAACG GAGAAGGGGC
    TCATCCATAC CCCAGTTTAC CAATTCCCCC ACAATGGTGA TCATGGTGGG TTTACCAGCT
    CGAGGCAAGA CCTATATCTC CACAAAGCTC ACACGATATC TCAACTGGAT AGGAACACCA
    ACTAAAGCAA GGACATTTTT TTGGCCACTG GGATCTTCCC AACCTCTTCA CTGGCACTCC
    CTGCTTTCAG TGTTTAATTT AGGCCAGTAT CGACGAGAGG CAGTGAGCTA CAAGAACTAT
    GAATTCTTTC TTCCAGACAA CATGGAAGCC CTGCAAATCA GGAAGCAGTG CGCCCTGGCA
    GCCCTGAAGG ATGTTCACAA CTATCTCAGC CATGAGGAAG GTCATGTTGC GGTTTTTGAT
    GCCACCAACA CTACCAGAGA ACGACGGTCA CTGATCCTGC AGTTTGCAAA AGAACATGGT
    TACAAGGTGT TTTTCATTGA GTCCATTTGT AATGACCCTG GCATAATTGC AGAAAACATC
    AGGCAAGTGA AACTTGGCAG CCCTGATTAT ATAGACTGTG ACCGGGAAAA GGTTCTGGAA
    GACTTTCTAA AGAGAATTGA GTGCTATGAG GTCAACTACC AACCCTTGGA TGAGGAACTG
    GACAGCCACC TGTCCTACAT CAAGATCTTC GACGTGGGCA CACGCTACAT GGTGAACCGA
    GTGCAGGATC ACATCCAGAG CCGCACAGTC TACTACCTCA TGAATATCCA TGTCACACCT
    CGCTCCATCT ACCTTTGCCG ACATGGCGAG AGTGAACTCA ACATCAGAGG CCGCATCGGA
    GGTGACTCTG GCCTCTCAGT TCGCGGCAAG CAGTATGCCT ATGCCCTGGC CAACTTCATT
    CAGTCCCAGG GCATCAGCTC CCTGAAGGTG TGGACCAGTC ACATGAAGAG GACCATCCAG
    ACAGCTGAGG CCCTGGGTGT CCCCTATGAG CAGTGGAAGG CCCTGAATGA GATTGATGCG
    GGTGTCTGTG AGGAGATGAC CTATGAAGAA ATCCAGGAAC ATTACCCTGA AGAATTTGCA
    CTGCGAGACC AAGATAAATA TCGCTACCGC TATCCCAAGG GAGAGTCCTA TGAGGATCTG
    GTTCAGCGTC TGGAGCCAGT GATAATGGAG CTAGAACGAC AGGAGAATGT ACTGGTGATC
    TGCCACCAGG CTGTCATGCG GTGCCTCCTG GCCTATTTCC TGGATAAAAG TTCAGATGAG
    CTTCCATATC TCAAGTGCCC TCTGCACACA GTGCTCAAAC TCACTCCTGT GGCTTATGGC
    TGCAAAGTGG AATCCATCTA CCTGAATGTG GAGGCCGTGA ACACACACCG GGAGAAGCCT
    GAGAATGTGG ACATCACCCG GGAACCTGAG GAAGCCCTGG ATACTGTCCC AGCCCACTAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016885065.1
    CDS389..1891
    Translation

    Target ORF information:

    RefSeq Version XM_017029576.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCATTCG GTGCGACTCC TAAGATGTCT CCAGAGATGG GAGAGCTCAC CCAAACCAGG 
    TTGCAGAAGA TCTGGATTCC ACACAGCAGC GGCAGCAGCA GGCTGCAACG GAGAAGGGGC
    TCATCCATAC CCCAGTTTAC CAATTCCCCC ACAATGGTGA TCATGGTGGG TTTACCAGCT
    CGAGGCAAGA CCTATATCTC CACAAAGCTC ACACGATATC TCAACTGGAT AGGAACACCA
    ACTAAAGCAA GGACATTTTT TTGGCCACTG GGATCTTCCC AACCTCTTCA CTGGCACTCC
    CTGCTTTCAG TGTTTAATTT AGGCCAGTAT CGACGAGAGG CAGTGAGCTA CAAGAACTAT
    GAATTCTTTC TTCCAGACAA CATGGAAGCC CTGCAAATCA GGAAGCAGTG CGCCCTGGCA
    GCCCTGAAGG ATGTTCACAA CTATCTCAGC CATGAGGAAG GTCATGTTGC GGTTTTTGAT
    GCCACCAACA CTACCAGAGA ACGACGGTCA CTGATCCTGC AGTTTGCAAA AGAACATGGT
    TACAAGGTGT TTTTCATTGA GTCCATTTGT AATGACCCTG GCATAATTGC AGAAAACATC
    AGGCAAGTGA AACTTGGCAG CCCTGATTAT ATAGACTGTG ACCGGGAAAA GGTTCTGGAA
    GACTTTCTAA AGAGAATTGA GTGCTATGAG GTCAACTACC AACCCTTGGA TGAGGAACTG
    GACAGCCACC TGTCCTACAT CAAGATCTTC GACGTGGGCA CACGCTACAT GGTGAACCGA
    GTGCAGGATC ACATCCAGAG CCGCACAGTC TACTACCTCA TGAATATCCA TGTCACACCT
    CGCTCCATCT ACCTTTGCCG ACATGGCGAG AGTGAACTCA ACATCAGAGG CCGCATCGGA
    GGTGACTCTG GCCTCTCAGT TCGCGGCAAG CAGTATGCCT ATGCCCTGGC CAACTTCATT
    CAGTCCCAGG GCATCAGCTC CCTGAAGGTG TGGACCAGTC ACATGAAGAG GACCATCCAG
    ACAGCTGAGG CCCTGGGTGT CCCCTATGAG CAGTGGAAGG CCCTGAATGA GATTGATGCG
    GGTGTCTGTG AGGAGATGAC CTATGAAGAA ATCCAGGAAC ATTACCCTGA AGAATTTGCA
    CTGCGAGACC AAGATAAATA TCGCTACCGC TATCCCAAGG GAGAGTCCTA TGAGGATCTG
    GTTCAGCGTC TGGAGCCAGT GATAATGGAG CTAGAACGAC AGGAGAATGT ACTGGTGATC
    TGCCACCAGG CTGTCATGCG GTGCCTCCTG GCCTATTTCC TGGATAAAAG TTCAGATGAG
    CTTCCATATC TCAAGTGCCC TCTGCACACA GTGCTCAAAC TCACTCCTGT GGCTTATGGC
    TGCAAAGTGG AATCCATCTA CCTGAATGTG GAGGCCGTGA ACACACACCG GGAGAAGCCT
    GAGAATGTGG ACATCACCCG GGAACCTGAG GAAGCCCTGG ATACTGTCCC AGCCCACTAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    [EXPRESSION OF GENES, WHICH CONTROL GLUCOSE METABOLISM, IN BLOOD CELLS OF THE OBESE BOYS WITH INSULIN RESISTANCE].
    Likars'ka sprava(3-4)66-71(2015 Apr-Jun)
    Tiazhka OV,Minchenko DO,Davydov VV,Moliavko OS,Budreiko OA,Kulieshova DK,Minchenko OH


    Perioperative genomic profiles using structure-specific oligonucleotide probes.
    Clinical medicine & research7(3)69-84(2009 Sep)
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    The DNA sequence of the human X chromosome.
    Nature434(7031)325-37(2005 Mar)
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    Tissue-specific structure/function differentiation of the liver isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
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