PFKFB1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PFKFB1
Entrez Gene ID	5207
Full Name	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Synonyms	F6PK,HL2K,PFRX
General protein information	Preferred Names 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 Names 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 6PF-2-K/Fru-2,6-P2ASE liver isozyme 6PF-2-K/Fru-2,6-P2ase 1 PFK/FBPase 1 fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.21
Summary	This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012].
Disorder MIM:	311790

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271805.1	NP_001258734.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 3
NM_001271804.2	NP_001258733.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 2
NM_002625.4	NP_002616.2	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 1
XM_017029578.1	XP_016885067.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X3
XM_024452389.1	XP_024308157.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X4
XM_017029577.1	XP_016885066.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X2
NM_002625.3	NP_002616.2	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 1
NM_001271804.1	NP_001258733.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 2
XM_017029576.1	XP_016885065.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100488795	XP_004919688.1
Bos taurus (cattle)	PFKFB1	NP_776997.3
Rattus norvegicus (Norway rat)	Pfkfb1	NP_036753.4
Danio rerio (zebrafish)	LOC100330415	XP_002666487.2
	PFKFB1	XP_001091907.2
Canis lupus familiaris (dog)	PFKFB1	XP_005641453.1
Mus musculus (house mouse)	Pfkfb1	NP_032850.1
Homo sapiens (human)	PFKFB1	NP_002616.2
Gallus gallus (chicken)	PFKFB4	NP_001025755.1

Gene Ontology
Bibliography

Related articles in PubMed

[EXPRESSION OF GENES, WHICH CONTROL GLUCOSE METABOLISM, IN BLOOD CELLS OF THE OBESE BOYS WITH INSULIN RESISTANCE].
Tiazhka OV, Minchenko DO, Davydov VV, Moliavko OS, Budreiko OA, Kulieshova DK, Minchenko OH
Likars'ka sprava(3-4)66-71(2015 Apr-Jun)

Perioperative genomic profiles using structure-specific oligonucleotide probes.
Hogan KJ, Burmester JK, Caldwell MD, Hogan QH, Coursin DB, Green DN, Selzer RM, Broderick TP, Rusy DA, Poroli M, Lutz AL, Sanders AM, Oldenburg MC, Koelbl JA, de Arruda-Indig M, Halsey JL, Day SP, Domanico MJ
Clinical medicine & research7(3)69-84(2009 Sep)

The DNA sequence of the human X chromosome.
Ross MT, Grafham DV, Coffey AJ, Scherer S, McLay K, Muzny D, Platzer M, Howell GR, Burrows C, Bird CP, Frankish A, Lovell FL, Howe KL, Ashurst JL, Fulton RS, Sudbrak R, Wen G, Jones MC, Hurles ME, Andrews TD, Scott CE, Searle S, Ramser J, Whittaker A, Deadman R, Carter NP, Hunt SE, Chen R, Cree A, Gunaratne P, Havlak P, Hodgson A, Metzker ML, Richards S, Scott G, Steffen D, Sodergren E, Wheeler DA, Worley KC, Ainscough R, Ambrose KD, Ansari-Lari MA, Aradhya S, Ashwell RI, Babbage AK, Bagguley CL, Ballabio A, Banerjee R, Barker GE, Barlow KF, Barrett IP, Bates KN, Beare DM, Beasley H, Beasley O, Beck A, Bethel G, Blechschmidt K, Brady N, Bray-Allen S, Bridgeman AM, Brown AJ, Brown MJ, Bonnin D, Bruford EA, Buhay C, Burch P, Burford D, Burgess J, Burrill W, Burton J, Bye JM, Carder C, Carrel L, Chako J, Chapman JC, Chavez D, Chen E, Chen G, Chen Y, Chen Z, Chinault C, Ciccodicola A, Clark SY, Clarke G, Clee CM, Clegg S, Clerc-Blankenburg K, Clifford K, Cobley V, Cole CG, Conquer JS, Corby N, Connor RE, David R, Davies J, Davis C, Davis J, Delgado O, Deshazo D, Dhami P, Ding Y, Dinh H, Dodsworth S, Draper H, Dugan-Rocha S, Dunham A, Dunn M, Durbin KJ, Dutta I, Eades T, Ellwood M, Emery-Cohen A, Errington H, Evans KL, Faulkner L, Francis F, Frankland J, Fraser AE, Galgoczy P, Gilbert J, Gill R, Gl?ckner G, Gregory SG, Gribble S, Griffiths C, Grocock R, Gu Y, Gwilliam R, Hamilton C, Hart EA, Hawes A, Heath PD, Heitmann K, Hennig S, Hernandez J, Hinzmann B, Ho S, Hoffs M, Howden PJ, Huckle EJ, Hume J, Hunt PJ, Hunt AR, Isherwood J, Jacob L, Johnson D, Jones S, de Jong PJ, Joseph SS, Keenan S, Kelly S, Kershaw JK, Khan Z, Kioschis P, Klages S, Knights AJ, Kosiura A, Kovar-Smith C, Laird GK, Langford C, Lawlor S, Leversha M, Lewis L, Liu W, Lloyd C, Lloyd DM, Loulseged H, Loveland JE, Lovell JD, Lozado R, Lu J, Lyne R, Ma J, Maheshwari M, Matthews LH, McDowall J, McLaren S, McMurray A, Meidl P, Meitinger T, Milne S, Miner G, Mistry SL, Morgan M, Morris S, M?ller I, Mullikin JC, Nguyen N, Nordsiek G, Nyakatura G, O'Dell CN, Okwuonu G, Palmer S, Pandian R, Parker D, Parrish J, Pasternak S, Patel D, Pearce AV, Pearson DM, Pelan SE, Perez L, Porter KM, Ramsey Y, Reichwald K, Rhodes S, Ridler KA, Schlessinger D, Schueler MG, Sehra HK, Shaw-Smith C, Shen H, Sheridan EM, Shownkeen R, Skuce CD, Smith ML, Sotheran EC, Steingruber HE, Steward CA, Storey R, Swann RM, Swarbreck D, Tabor PE, Taudien S, Taylor T, Teague B, Thomas K, Thorpe A, Timms K, Tracey A, Trevanion S, Tromans AC, d'Urso M, Verduzco D, Villasana D, Waldron L, Wall M, Wang Q, Warren J, Warry GL, Wei X, West A, Whitehead SL, Whiteley MN, Wilkinson JE, Willey DL, Williams G, Williams L, Williamson A, Williamson H, Wilming L, Woodmansey RL, Wray PW, Yen J, Zhang J, Zhou J, Zoghbi H, Zorilla S, Buck D, Reinhardt R, Poustka A, Rosenthal A, Lehrach H, Meindl A, Minx PJ, Hillier LW, Willard HF, Wilson RK, Waterston RH, Rice CM, Vaudin M, Coulson A, Nelson DL, Weinstock G, Sulston JE, Durbin R, Hubbard T, Gibbs RA, Beck S, Rogers J, Bentley DR
Nature434(7031)325-37(2005 Mar)

A humble hexose monophosphate pathway metabolite regulates short- and long-term control of lipogenesis.
Veech RL
Proceedings of the National Academy of Sciences of the United States of America100(10)5578-80(2003 May)

Tissue-specific structure/function differentiation of the liver isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Lee YH, Li Y, Uyeda K, Hasemann CA
The Journal of biological chemistry278(1)523-30(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Insulin resistance in obese boys leads to up-regulation of INSIG2 gene expression as well as to down-regulation of PFKFB1, PFKFB3, and HK2 genes in the blood cells as compared to obese patients with normal insulin sensitivity.
Title: [EXPRESSION OF GENES, WHICH CONTROL GLUCOSE METABOLISM, IN BLOOD CELLS OF THE OBESE BOYS WITH INSULIN RESISTANCE].

Observational study of genetic testing. (HuGE Navigator)
Title: Perioperative genomic profiles using structure-specific oligonucleotide probes.

Results describe structural differences between two isozymes of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from liver and testis.
Title: Tissue-specific structure/function differentiation of the liver isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.

The following PFKFB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFKFB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20318	NM_002625.4 Latest version!	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu03896	NM_001271805.1 Latest version!	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu09773	NM_001271804.2 Latest version!	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu54096	XM_024452389.1 Latest version!	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu99334	XM_017029577.1 Latest version!	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu99335	XM_017029578.1 Latest version!	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu99333	XM_017029576.1 Latest version!	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu20318	NM_002625.3	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu09773	NM_001271804.1	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PFKFB1 ( NM_002625.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002616.2 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20318
Clone ID Related Accession (Same CDS sequence)	NM_002625.4 , NM_002625.3
Accession Version	NM_002625.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL020991.1, X52638.1, AK314089.1, AL703826.1 and AL049732.11. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_002625.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189664.85930.1, X52638.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved MANE Ensembl match :: ENST00000375006.8/ ENSP00000364145.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT 
TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA 
AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGTGTTTAA TTTAGGCCAG 
TATCGACGAG AGGCAGTGAG CTACAAGAAC TATGAATTCT TTCTTCCAGA CAACATGGAA 
GCCCTGCAAA TCAGGAAGCA GTGCGCCCTG GCAGCCCTGA AGGATGTTCA CAACTATCTC 
AGCCATGAGG AAGGTCATGT TGCGGTTTTT GATGCCACCA ACACTACCAG AGAACGACGG 
TCACTGATCC TGCAGTTTGC AAAAGAACAT GGTTACAAGG TGTTTTTCAT TGAGTCCATT 
TGTAATGACC CTGGCATAAT TGCAGAAAAC ATCAGGCAAG TGAAACTTGG CAGCCCTGAT 
TATATAGACT GTGACCGGGA AAAGGTTCTG GAAGACTTTC TAAAGAGAAT TGAGTGCTAT 
GAGGTCAACT ACCAACCCTT GGATGAGGAA CTGGACAGCC ACCTGTCCTA CATCAAGATC 
TTCGACGTGG GCACACGCTA CATGGTGAAC CGAGTGCAGG ATCACATCCA GAGCCGCACA 
GTCTACTACC TCATGAATAT CCATGTCACA CCTCGCTCCA TCTACCTTTG CCGACATGGC 
GAGAGTGAAC TCAACATCAG AGGCCGCATC GGAGGTGACT CTGGCCTCTC AGTTCGCGGC 
AAGCAGTATG CCTATGCCCT GGCCAACTTC ATTCAGTCCC AGGGCATCAG CTCCCTGAAG 
GTGTGGACCA GTCACATGAA GAGGACCATC CAGACAGCTG AGGCCCTGGG TGTCCCCTAT 
GAGCAGTGGA AGGCCCTGAA TGAGATTGAT GCGGGTGTCT GTGAGGAGAT GACCTATGAA 
GAAATCCAGG AACATTACCC TGAAGAATTT GCACTGCGAG ACCAAGATAA ATATCGCTAC 
CGCTATCCCA AGGGAGAGTC CTATGAGGAT CTGGTTCAGC GTCTGGAGCC AGTGATAATG 
GAGCTAGAAC GACAGGAGAA TGTACTGGTG ATCTGCCACC AGGCTGTCAT GCGGTGCCTC 
CTGGCCTATT TCCTGGATAA AAGTTCAGAT GAGCTTCCAT ATCTCAAGTG CCCTCTGCAC 
ACAGTGCTCA AACTCACTCC TGTGGCTTAT GGCTGCAAAG TGGAATCCAT CTACCTGAAT 
GTGGAGGCCG TGAACACACA CCGGGAGAAG CCTGAGAATG TGGACATCAC CCGGGAACCT 
GAGGAAGCCC TGGATACTGT CCCAGCCCAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002616.2
CDS	182..1597
Translation	MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY

Target ORF information:

RefSeq Version	NM_002625.4
Organism	Homo sapiens(human)
Definition	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002625.4

ORF Insert Sequence:

ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT 
TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA 
AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGTGTTTAA TTTAGGCCAG 
TATCGACGAG AGGCAGTGAG CTACAAGAAC TATGAATTCT TTCTTCCAGA CAACATGGAA 
GCCCTGCAAA TCAGGAAGCA GTGCGCCCTG GCAGCCCTGA AGGATGTTCA CAACTATCTC 
AGCCATGAGG AAGGTCATGT TGCGGTTTTT GATGCCACCA ACACTACCAG AGAACGACGG 
TCACTGATCC TGCAGTTTGC AAAAGAACAT GGTTACAAGG TGTTTTTCAT TGAGTCCATT 
TGTAATGACC CTGGCATAAT TGCAGAAAAC ATCAGGCAAG TGAAACTTGG CAGCCCTGAT 
TATATAGACT GTGACCGGGA AAAGGTTCTG GAAGACTTTC TAAAGAGAAT TGAGTGCTAT 
GAGGTCAACT ACCAACCCTT GGATGAGGAA CTGGACAGCC ACCTGTCCTA CATCAAGATC 
TTCGACGTGG GCACACGCTA CATGGTGAAC CGAGTGCAGG ATCACATCCA GAGCCGCACA 
GTCTACTACC TCATGAATAT CCATGTCACA CCTCGCTCCA TCTACCTTTG CCGACATGGC 
GAGAGTGAAC TCAACATCAG AGGCCGCATC GGAGGTGACT CTGGCCTCTC AGTTCGCGGC 
AAGCAGTATG CCTATGCCCT GGCCAACTTC ATTCAGTCCC AGGGCATCAG CTCCCTGAAG 
GTGTGGACCA GTCACATGAA GAGGACCATC CAGACAGCTG AGGCCCTGGG TGTCCCCTAT 
GAGCAGTGGA AGGCCCTGAA TGAGATTGAT GCGGGTGTCT GTGAGGAGAT GACCTATGAA 
GAAATCCAGG AACATTACCC TGAAGAATTT GCACTGCGAG ACCAAGATAA ATATCGCTAC 
CGCTATCCCA AGGGAGAGTC CTATGAGGAT CTGGTTCAGC GTCTGGAGCC AGTGATAATG 
GAGCTAGAAC GACAGGAGAA TGTACTGGTG ATCTGCCACC AGGCTGTCAT GCGGTGCCTC 
CTGGCCTATT TCCTGGATAA AAGTTCAGAT GAGCTTCCAT ATCTCAAGTG CCCTCTGCAC 
ACAGTGCTCA AACTCACTCC TGTGGCTTAT GGCTGCAAAG TGGAATCCAT CTACCTGAAT 
GTGGAGGCCG TGAACACACA CCGGGAGAAG CCTGAGAATG TGGACATCAC CCGGGAACCT 
GAGGAAGCCC TGGATACTGT CCCAGCCCAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKFB1 ( NM_002625.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002616.2 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20318
Clone ID Related Accession (Same CDS sequence)	NM_002625.4 , NM_002625.3
Accession Version	NM_002625.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X52638.1, AK314089.1 and AL703826.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2012 this sequence version replaced NM_002625.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X52638.1, BC096078.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT 
TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA 
AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGTGTTTAA TTTAGGCCAG 
TATCGACGAG AGGCAGTGAG CTACAAGAAC TATGAATTCT TTCTTCCAGA CAACATGGAA 
GCCCTGCAAA TCAGGAAGCA GTGCGCCCTG GCAGCCCTGA AGGATGTTCA CAACTATCTC 
AGCCATGAGG AAGGTCATGT TGCGGTTTTT GATGCCACCA ACACTACCAG AGAACGACGG 
TCACTGATCC TGCAGTTTGC AAAAGAACAT GGTTACAAGG TGTTTTTCAT TGAGTCCATT 
TGTAATGACC CTGGCATAAT TGCAGAAAAC ATCAGGCAAG TGAAACTTGG CAGCCCTGAT 
TATATAGACT GTGACCGGGA AAAGGTTCTG GAAGACTTTC TAAAGAGAAT TGAGTGCTAT 
GAGGTCAACT ACCAACCCTT GGATGAGGAA CTGGACAGCC ACCTGTCCTA CATCAAGATC 
TTCGACGTGG GCACACGCTA CATGGTGAAC CGAGTGCAGG ATCACATCCA GAGCCGCACA 
GTCTACTACC TCATGAATAT CCATGTCACA CCTCGCTCCA TCTACCTTTG CCGACATGGC 
GAGAGTGAAC TCAACATCAG AGGCCGCATC GGAGGTGACT CTGGCCTCTC AGTTCGCGGC 
AAGCAGTATG CCTATGCCCT GGCCAACTTC ATTCAGTCCC AGGGCATCAG CTCCCTGAAG 
GTGTGGACCA GTCACATGAA GAGGACCATC CAGACAGCTG AGGCCCTGGG TGTCCCCTAT 
GAGCAGTGGA AGGCCCTGAA TGAGATTGAT GCGGGTGTCT GTGAGGAGAT GACCTATGAA 
GAAATCCAGG AACATTACCC TGAAGAATTT GCACTGCGAG ACCAAGATAA ATATCGCTAC 
CGCTATCCCA AGGGAGAGTC CTATGAGGAT CTGGTTCAGC GTCTGGAGCC AGTGATAATG 
GAGCTAGAAC GACAGGAGAA TGTACTGGTG ATCTGCCACC AGGCTGTCAT GCGGTGCCTC 
CTGGCCTATT TCCTGGATAA AAGTTCAGAT GAGCTTCCAT ATCTCAAGTG CCCTCTGCAC 
ACAGTGCTCA AACTCACTCC TGTGGCTTAT GGCTGCAAAG TGGAATCCAT CTACCTGAAT 
GTGGAGGCCG TGAACACACA CCGGGAGAAG CCTGAGAATG TGGACATCAC CCGGGAACCT 
GAGGAAGCCC TGGATACTGT CCCAGCCCAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002616.2
CDS	72..1487
Translation	MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY

Target ORF information:

RefSeq Version	NM_002625.3
Organism	Homo sapiens(human)
Definition	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002625.3

ORF Insert Sequence:

ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT 
TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA 
AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGTGTTTAA TTTAGGCCAG 
TATCGACGAG AGGCAGTGAG CTACAAGAAC TATGAATTCT TTCTTCCAGA CAACATGGAA 
GCCCTGCAAA TCAGGAAGCA GTGCGCCCTG GCAGCCCTGA AGGATGTTCA CAACTATCTC 
AGCCATGAGG AAGGTCATGT TGCGGTTTTT GATGCCACCA ACACTACCAG AGAACGACGG 
TCACTGATCC TGCAGTTTGC AAAAGAACAT GGTTACAAGG TGTTTTTCAT TGAGTCCATT 
TGTAATGACC CTGGCATAAT TGCAGAAAAC ATCAGGCAAG TGAAACTTGG CAGCCCTGAT 
TATATAGACT GTGACCGGGA AAAGGTTCTG GAAGACTTTC TAAAGAGAAT TGAGTGCTAT 
GAGGTCAACT ACCAACCCTT GGATGAGGAA CTGGACAGCC ACCTGTCCTA CATCAAGATC 
TTCGACGTGG GCACACGCTA CATGGTGAAC CGAGTGCAGG ATCACATCCA GAGCCGCACA 
GTCTACTACC TCATGAATAT CCATGTCACA CCTCGCTCCA TCTACCTTTG CCGACATGGC 
GAGAGTGAAC TCAACATCAG AGGCCGCATC GGAGGTGACT CTGGCCTCTC AGTTCGCGGC 
AAGCAGTATG CCTATGCCCT GGCCAACTTC ATTCAGTCCC AGGGCATCAG CTCCCTGAAG 
GTGTGGACCA GTCACATGAA GAGGACCATC CAGACAGCTG AGGCCCTGGG TGTCCCCTAT 
GAGCAGTGGA AGGCCCTGAA TGAGATTGAT GCGGGTGTCT GTGAGGAGAT GACCTATGAA 
GAAATCCAGG AACATTACCC TGAAGAATTT GCACTGCGAG ACCAAGATAA ATATCGCTAC 
CGCTATCCCA AGGGAGAGTC CTATGAGGAT CTGGTTCAGC GTCTGGAGCC AGTGATAATG 
GAGCTAGAAC GACAGGAGAA TGTACTGGTG ATCTGCCACC AGGCTGTCAT GCGGTGCCTC 
CTGGCCTATT TCCTGGATAA AAGTTCAGAT GAGCTTCCAT ATCTCAAGTG CCCTCTGCAC 
ACAGTGCTCA AACTCACTCC TGTGGCTTAT GGCTGCAAAG TGGAATCCAT CTACCTGAAT 
GTGGAGGCCG TGAACACACA CCGGGAGAAG CCTGAGAATG TGGACATCAC CCGGGAACCT 
GAGGAAGCCC TGGATACTGT CCCAGCCCAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKFB1 ( NM_001271805.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258734.1 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu03896
Clone ID Related Accession (Same CDS sequence)	NM_001271805.1
Accession Version	NM_001271805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300724.1. Transcript Variant: This variant (3) lacks two 5' exons and contains an alternate 5' exon, compared to variant 1. The resulting isoform (3) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300724.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGAAA AAACCTCTAG AATAAAAGTG TTTAATTTAG GCCAGTATCG ACGAGAGGCA 
GTGAGCTACA AGAACTATGA ATTCTTTCTT CCAGACAACA TGGAAGCCCT GCAAATCAGG 
AAGCAGTGCG CCCTGGCAGC CCTGAAGGAT GTTCACAACT ATCTCAGCCA TGAGGAAGGT 
CATGTTGCGG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATCCTGCAG 
TTTGCAAAAG AACATGGTTA CAAGGTGTTT TTCATTGAGT CCATTTGTAA TGACCCTGGC 
ATAATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGCC CTGATTATAT AGACTGTGAC 
CGGGAAAAGG TTCTGGAAGA CTTTCTAAAG AGAATTGAGT GCTATGAGGT CAACTACCAA 
CCCTTGGATG AGGAACTGGA CAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA 
CGCTACATGG TGAACCGAGT GCAGGATCAC ATCCAGAGCC GCACAGTCTA CTACCTCATG 
AATATCCATG TCACACCTCG CTCCATCTAC CTTTGCCGAC ATGGCGAGAG TGAACTCAAC 
ATCAGAGGCC GCATCGGAGG TGACTCTGGC CTCTCAGTTC GCGGCAAGCA GTATGCCTAT 
GCCCTGGCCA ACTTCATTCA GTCCCAGGGC ATCAGCTCCC TGAAGGTGTG GACCAGTCAC 
ATGAAGAGGA CCATCCAGAC AGCTGAGGCC CTGGGTGTCC CCTATGAGCA GTGGAAGGCC 
CTGAATGAGA TTGATGCGGG TGTCTGTGAG GAGATGACCT ATGAAGAAAT CCAGGAACAT 
TACCCTGAAG AATTTGCACT GCGAGACCAA GATAAATATC GCTACCGCTA TCCCAAGGGA 
GAGTCCTATG AGGATCTGGT TCAGCGTCTG GAGCCAGTGA TAATGGAGCT AGAACGACAG 
GAGAATGTAC TGGTGATCTG CCACCAGGCT GTCATGCGGT GCCTCCTGGC CTATTTCCTG 
GATAAAAGTT CAGATGAGCT TCCATATCTC AAGTGCCCTC TGCACACAGT GCTCAAACTC 
ACTCCTGTGG CTTATGGCTG CAAAGTGGAA TCCATCTACC TGAATGTGGA GGCCGTGAAC 
ACACACCGGG AGAAGCCTGA GAATGTGGAC ATCACCCGGG AACCTGAGGA AGCCCTGGAT 
ACTGTCCCAG CCCACTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258734.1
CDS	102..1322
Translation	MEEKTSRIKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY

Target ORF information:

RefSeq Version	NM_001271805.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271805.1

ORF Insert Sequence:

ATGGAAGAAA AAACCTCTAG AATAAAAGTG TTTAATTTAG GCCAGTATCG ACGAGAGGCA 
GTGAGCTACA AGAACTATGA ATTCTTTCTT CCAGACAACA TGGAAGCCCT GCAAATCAGG 
AAGCAGTGCG CCCTGGCAGC CCTGAAGGAT GTTCACAACT ATCTCAGCCA TGAGGAAGGT 
CATGTTGCGG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATCCTGCAG 
TTTGCAAAAG AACATGGTTA CAAGGTGTTT TTCATTGAGT CCATTTGTAA TGACCCTGGC 
ATAATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGCC CTGATTATAT AGACTGTGAC 
CGGGAAAAGG TTCTGGAAGA CTTTCTAAAG AGAATTGAGT GCTATGAGGT CAACTACCAA 
CCCTTGGATG AGGAACTGGA CAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA 
CGCTACATGG TGAACCGAGT GCAGGATCAC ATCCAGAGCC GCACAGTCTA CTACCTCATG 
AATATCCATG TCACACCTCG CTCCATCTAC CTTTGCCGAC ATGGCGAGAG TGAACTCAAC 
ATCAGAGGCC GCATCGGAGG TGACTCTGGC CTCTCAGTTC GCGGCAAGCA GTATGCCTAT 
GCCCTGGCCA ACTTCATTCA GTCCCAGGGC ATCAGCTCCC TGAAGGTGTG GACCAGTCAC 
ATGAAGAGGA CCATCCAGAC AGCTGAGGCC CTGGGTGTCC CCTATGAGCA GTGGAAGGCC 
CTGAATGAGA TTGATGCGGG TGTCTGTGAG GAGATGACCT ATGAAGAAAT CCAGGAACAT 
TACCCTGAAG AATTTGCACT GCGAGACCAA GATAAATATC GCTACCGCTA TCCCAAGGGA 
GAGTCCTATG AGGATCTGGT TCAGCGTCTG GAGCCAGTGA TAATGGAGCT AGAACGACAG 
GAGAATGTAC TGGTGATCTG CCACCAGGCT GTCATGCGGT GCCTCCTGGC CTATTTCCTG 
GATAAAAGTT CAGATGAGCT TCCATATCTC AAGTGCCCTC TGCACACAGT GCTCAAACTC 
ACTCCTGTGG CTTATGGCTG CAAAGTGGAA TCCATCTACC TGAATGTGGA GGCCGTGAAC 
ACACACCGGG AGAAGCCTGA GAATGTGGAC ATCACCCGGG AACCTGAGGA AGCCCTGGAT 
ACTGTCCCAG CCCACTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKFB1 ( NM_001271804.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258733.1 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09773
Clone ID Related Accession (Same CDS sequence)	NM_001271804.2 , NM_001271804.1
Accession Version	NM_001271804.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL020991.1, AK314089.1, JQ425695.1, AK300724.1 and AL049732.11. On May 31, 2019 this sequence version replaced NM_001271804.1. Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JQ425695.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT 
TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA 
AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGACAACAT GGAAGCCCTG 
CAAATCAGGA AGCAGTGCGC CCTGGCAGCC CTGAAGGATG TTCACAACTA TCTCAGCCAT 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGGTCACTG 
ATCCTGCAGT TTGCAAAAGA ACATGGTTAC AAGGTGTTTT TCATTGAGTC CATTTGTAAT 
GACCCTGGCA TAATTGCAGA AAACATCAGG CAAGTGAAAC TTGGCAGCCC TGATTATATA 
GACTGTGACC GGGAAAAGGT TCTGGAAGAC TTTCTAAAGA GAATTGAGTG CTATGAGGTC 
AACTACCAAC CCTTGGATGA GGAACTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT GAACCGAGTG CAGGATCACA TCCAGAGCCG CACAGTCTAC 
TACCTCATGA ATATCCATGT CACACCTCGC TCCATCTACC TTTGCCGACA TGGCGAGAGT 
GAACTCAACA TCAGAGGCCG CATCGGAGGT GACTCTGGCC TCTCAGTTCG CGGCAAGCAG 
TATGCCTATG CCCTGGCCAA CTTCATTCAG TCCCAGGGCA TCAGCTCCCT GAAGGTGTGG 
ACCAGTCACA TGAAGAGGAC CATCCAGACA GCTGAGGCCC TGGGTGTCCC CTATGAGCAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAGG AGATGACCTA TGAAGAAATC 
CAGGAACATT ACCCTGAAGA ATTTGCACTG CGAGACCAAG ATAAATATCG CTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTGG AGCCAGTGAT AATGGAGCTA 
GAACGACAGG AGAATGTACT GGTGATCTGC CACCAGGCTG TCATGCGGTG CCTCCTGGCC 
TATTTCCTGG ATAAAAGTTC AGATGAGCTT CCATATCTCA AGTGCCCTCT GCACACAGTG 
CTCAAACTCA CTCCTGTGGC TTATGGCTGC AAAGTGGAAT CCATCTACCT GAATGTGGAG 
GCCGTGAACA CACACCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAGGAA 
GCCCTGGATA CTGTCCCAGC CCACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258733.1
CDS	182..1531
Translation	MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY

Target ORF information:

RefSeq Version	NM_001271804.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271804.2

ORF Insert Sequence:

ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT 
TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA 
AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGACAACAT GGAAGCCCTG 
CAAATCAGGA AGCAGTGCGC CCTGGCAGCC CTGAAGGATG TTCACAACTA TCTCAGCCAT 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGGTCACTG 
ATCCTGCAGT TTGCAAAAGA ACATGGTTAC AAGGTGTTTT TCATTGAGTC CATTTGTAAT 
GACCCTGGCA TAATTGCAGA AAACATCAGG CAAGTGAAAC TTGGCAGCCC TGATTATATA 
GACTGTGACC GGGAAAAGGT TCTGGAAGAC TTTCTAAAGA GAATTGAGTG CTATGAGGTC 
AACTACCAAC CCTTGGATGA GGAACTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT GAACCGAGTG CAGGATCACA TCCAGAGCCG CACAGTCTAC 
TACCTCATGA ATATCCATGT CACACCTCGC TCCATCTACC TTTGCCGACA TGGCGAGAGT 
GAACTCAACA TCAGAGGCCG CATCGGAGGT GACTCTGGCC TCTCAGTTCG CGGCAAGCAG 
TATGCCTATG CCCTGGCCAA CTTCATTCAG TCCCAGGGCA TCAGCTCCCT GAAGGTGTGG 
ACCAGTCACA TGAAGAGGAC CATCCAGACA GCTGAGGCCC TGGGTGTCCC CTATGAGCAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAGG AGATGACCTA TGAAGAAATC 
CAGGAACATT ACCCTGAAGA ATTTGCACTG CGAGACCAAG ATAAATATCG CTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTGG AGCCAGTGAT AATGGAGCTA 
GAACGACAGG AGAATGTACT GGTGATCTGC CACCAGGCTG TCATGCGGTG CCTCCTGGCC 
TATTTCCTGG ATAAAAGTTC AGATGAGCTT CCATATCTCA AGTGCCCTCT GCACACAGTG 
CTCAAACTCA CTCCTGTGGC TTATGGCTGC AAAGTGGAAT CCATCTACCT GAATGTGGAG 
GCCGTGAACA CACACCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAGGAA 
GCCCTGGATA CTGTCCCAGC CCACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKFB1 ( NM_001271804.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258733.1 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09773
Clone ID Related Accession (Same CDS sequence)	NM_001271804.2 , NM_001271804.1
Accession Version	NM_001271804.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314089.1, JQ425695.1 and AK300724.1. Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JQ425695.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT 
TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA 
AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGACAACAT GGAAGCCCTG 
CAAATCAGGA AGCAGTGCGC CCTGGCAGCC CTGAAGGATG TTCACAACTA TCTCAGCCAT 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGGTCACTG 
ATCCTGCAGT TTGCAAAAGA ACATGGTTAC AAGGTGTTTT TCATTGAGTC CATTTGTAAT 
GACCCTGGCA TAATTGCAGA AAACATCAGG CAAGTGAAAC TTGGCAGCCC TGATTATATA 
GACTGTGACC GGGAAAAGGT TCTGGAAGAC TTTCTAAAGA GAATTGAGTG CTATGAGGTC 
AACTACCAAC CCTTGGATGA GGAACTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT GAACCGAGTG CAGGATCACA TCCAGAGCCG CACAGTCTAC 
TACCTCATGA ATATCCATGT CACACCTCGC TCCATCTACC TTTGCCGACA TGGCGAGAGT 
GAACTCAACA TCAGAGGCCG CATCGGAGGT GACTCTGGCC TCTCAGTTCG CGGCAAGCAG 
TATGCCTATG CCCTGGCCAA CTTCATTCAG TCCCAGGGCA TCAGCTCCCT GAAGGTGTGG 
ACCAGTCACA TGAAGAGGAC CATCCAGACA GCTGAGGCCC TGGGTGTCCC CTATGAGCAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAGG AGATGACCTA TGAAGAAATC 
CAGGAACATT ACCCTGAAGA ATTTGCACTG CGAGACCAAG ATAAATATCG CTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTGG AGCCAGTGAT AATGGAGCTA 
GAACGACAGG AGAATGTACT GGTGATCTGC CACCAGGCTG TCATGCGGTG CCTCCTGGCC 
TATTTCCTGG ATAAAAGTTC AGATGAGCTT CCATATCTCA AGTGCCCTCT GCACACAGTG 
CTCAAACTCA CTCCTGTGGC TTATGGCTGC AAAGTGGAAT CCATCTACCT GAATGTGGAG 
GCCGTGAACA CACACCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAGGAA 
GCCCTGGATA CTGTCCCAGC CCACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258733.1
CDS	72..1421
Translation	MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY

Target ORF information:

RefSeq Version	NM_001271804.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271804.1

ORF Insert Sequence:

ATGTCTCCAG AGATGGGAGA GCTCACCCAA ACCAGGTTGC AGAAGATCTG GATTCCACAC 
AGCAGCGGCA GCAGCAGGCT GCAACGGAGA AGGGGCTCAT CCATACCCCA GTTTACCAAT 
TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA TATCTCCACA 
AAGCTCACAC GATATCTCAA CTGGATAGGA ACACCAACTA AAGACAACAT GGAAGCCCTG 
CAAATCAGGA AGCAGTGCGC CCTGGCAGCC CTGAAGGATG TTCACAACTA TCTCAGCCAT 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGGTCACTG 
ATCCTGCAGT TTGCAAAAGA ACATGGTTAC AAGGTGTTTT TCATTGAGTC CATTTGTAAT 
GACCCTGGCA TAATTGCAGA AAACATCAGG CAAGTGAAAC TTGGCAGCCC TGATTATATA 
GACTGTGACC GGGAAAAGGT TCTGGAAGAC TTTCTAAAGA GAATTGAGTG CTATGAGGTC 
AACTACCAAC CCTTGGATGA GGAACTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT GAACCGAGTG CAGGATCACA TCCAGAGCCG CACAGTCTAC 
TACCTCATGA ATATCCATGT CACACCTCGC TCCATCTACC TTTGCCGACA TGGCGAGAGT 
GAACTCAACA TCAGAGGCCG CATCGGAGGT GACTCTGGCC TCTCAGTTCG CGGCAAGCAG 
TATGCCTATG CCCTGGCCAA CTTCATTCAG TCCCAGGGCA TCAGCTCCCT GAAGGTGTGG 
ACCAGTCACA TGAAGAGGAC CATCCAGACA GCTGAGGCCC TGGGTGTCCC CTATGAGCAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAGG AGATGACCTA TGAAGAAATC 
CAGGAACATT ACCCTGAAGA ATTTGCACTG CGAGACCAAG ATAAATATCG CTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTGG AGCCAGTGAT AATGGAGCTA 
GAACGACAGG AGAATGTACT GGTGATCTGC CACCAGGCTG TCATGCGGTG CCTCCTGGCC 
TATTTCCTGG ATAAAAGTTC AGATGAGCTT CCATATCTCA AGTGCCCTCT GCACACAGTG 
CTCAAACTCA CTCCTGTGGC TTATGGCTGC AAAGTGGAAT CCATCTACCT GAATGTGGAG 
GCCGTGAACA CACACCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAGGAA 
GCCCTGGATA CTGTCCCAGC CCACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKFB1 ( XM_024452389.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308157.1 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu54096
Clone ID Related Accession (Same CDS sequence)	XM_024452389.1
Accession Version	XM_024452389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA AAACCTCTAG AATAAAAGCA TCCATACCCC AGTTTACCAA TTCCCCCACA 
ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGCAAGACCT ATATCTCCAC AAAGCTCACA 
CGATATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGCCA GTATCGACGA 
GAGGCAGTGA GCTACAAGAA CTATGAATTC TTTCTTCCAG ACAACATGGA AGCCCTGCAA 
ATCAGGAAGC AGTGCGCCCT GGCAGCCCTG AAGGATGTTC ACAACTATCT CAGCCATGAG 
GAAGGTCATG TTGCGGTTTT TGATGCCACC AACACTACCA GAGAACGACG GTCACTGATC 
CTGCAGTTTG CAAAAGAACA TGGTTACAAG GTGTTTTTCA TTGAGTCCAT TTGTAATGAC 
CCTGGCATAA TTGCAGAAAA CATCAGGCAA GTGAAACTTG GCAGCCCTGA TTATATAGAC 
TGTGACCGGG AAAAGGTTCT GGAAGACTTT CTAAAGAGAA TTGAGTGCTA TGAGGTCAAC 
TACCAACCCT TGGATGAGGA ACTGGACAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG 
GGCACACGCT ACATGGTGAA CCGAGTGCAG GATCACATCC AGAGCCGCAC AGTCTACTAC 
CTCATGAATA TCCATGTCAC ACCTCGCTCC ATCTACCTTT GCCGACATGG CGAGAGTGAA 
CTCAACATCA GAGGCCGCAT CGGAGGTGAC TCTGGCCTCT CAGTTCGCGG CAAGCAGTAT 
GCCTATGCCC TGGCCAACTT CATTCAGTCC CAGGGCATCA GCTCCCTGAA GGTGTGGACC 
AGTCACATGA AGAGGACCAT CCAGACAGCT GAGGCCCTGG GTGTCCCCTA TGAGCAGTGG 
AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAGGAGA TGACCTATGA AGAAATCCAG 
GAACATTACC CTGAAGAATT TGCACTGCGA GACCAAGATA AATATCGCTA CCGCTATCCC 
AAGGGAGAGT CCTATGAGGA TCTGGTTCAG CGTCTGGAGC CAGTGATAAT GGAGCTAGAA 
CGACAGGAGA ATGTACTGGT GATCTGCCAC CAGGCTGTCA TGCGGTGCCT CCTGGCCTAT 
TTCCTGGATA AAAGTTCAGA TGAGCTTCCA TATCTCAAGT GCCCTCTGCA CACAGTGCTC 
AAACTCACTC CTGTGGCTTA TGGCTGCAAA GTGGAATCCA TCTACCTGAA TGTGGAGGCC 
GTGAACACAC ACCGGGAGAA GCCTGAGAAT GTGGACATCA CCCGGGAACC TGAGGAAGCC 
CTGGATACTG TCCCAGCCCA CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308157.1
CDS	1620..2966
Translation	MEEKTSRIKASIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY

Target ORF information:

RefSeq Version	XM_024452389.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452389.1

ORF Insert Sequence:

ATGGAAGAAA AAACCTCTAG AATAAAAGCA TCCATACCCC AGTTTACCAA TTCCCCCACA 
ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGCAAGACCT ATATCTCCAC AAAGCTCACA 
CGATATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGCCA GTATCGACGA 
GAGGCAGTGA GCTACAAGAA CTATGAATTC TTTCTTCCAG ACAACATGGA AGCCCTGCAA 
ATCAGGAAGC AGTGCGCCCT GGCAGCCCTG AAGGATGTTC ACAACTATCT CAGCCATGAG 
GAAGGTCATG TTGCGGTTTT TGATGCCACC AACACTACCA GAGAACGACG GTCACTGATC 
CTGCAGTTTG CAAAAGAACA TGGTTACAAG GTGTTTTTCA TTGAGTCCAT TTGTAATGAC 
CCTGGCATAA TTGCAGAAAA CATCAGGCAA GTGAAACTTG GCAGCCCTGA TTATATAGAC 
TGTGACCGGG AAAAGGTTCT GGAAGACTTT CTAAAGAGAA TTGAGTGCTA TGAGGTCAAC 
TACCAACCCT TGGATGAGGA ACTGGACAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG 
GGCACACGCT ACATGGTGAA CCGAGTGCAG GATCACATCC AGAGCCGCAC AGTCTACTAC 
CTCATGAATA TCCATGTCAC ACCTCGCTCC ATCTACCTTT GCCGACATGG CGAGAGTGAA 
CTCAACATCA GAGGCCGCAT CGGAGGTGAC TCTGGCCTCT CAGTTCGCGG CAAGCAGTAT 
GCCTATGCCC TGGCCAACTT CATTCAGTCC CAGGGCATCA GCTCCCTGAA GGTGTGGACC 
AGTCACATGA AGAGGACCAT CCAGACAGCT GAGGCCCTGG GTGTCCCCTA TGAGCAGTGG 
AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAGGAGA TGACCTATGA AGAAATCCAG 
GAACATTACC CTGAAGAATT TGCACTGCGA GACCAAGATA AATATCGCTA CCGCTATCCC 
AAGGGAGAGT CCTATGAGGA TCTGGTTCAG CGTCTGGAGC CAGTGATAAT GGAGCTAGAA 
CGACAGGAGA ATGTACTGGT GATCTGCCAC CAGGCTGTCA TGCGGTGCCT CCTGGCCTAT 
TTCCTGGATA AAAGTTCAGA TGAGCTTCCA TATCTCAAGT GCCCTCTGCA CACAGTGCTC 
AAACTCACTC CTGTGGCTTA TGGCTGCAAA GTGGAATCCA TCTACCTGAA TGTGGAGGCC 
GTGAACACAC ACCGGGAGAA GCCTGAGAAT GTGGACATCA CCCGGGAACC TGAGGAAGCC 
CTGGATACTG TCCCAGCCCA CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKFB1 ( XM_017029577.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885066.1 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu99334
Clone ID Related Accession (Same CDS sequence)	XM_017029577.1
Accession Version	XM_017029577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATTCG GTGCGACTCC TAAGATGTCT CCAGAGATGG GAGAGCTCAC CCAAACCAGG 
TTGCAGAAGA TCTGGATTCC ACACAGCAGC GGCAGCAGCA GGCTGCAACG GAGAAGGGGC 
TCATCCATAC CCCAGTTTAC CAATTCCCCC ACAATGGTGA TCATGGTGGG TTTACCAGCT 
CGAGGCAAGA CCTATATCTC CACAAAGCTC ACACGATATC TCAACTGGAT AGGAACACCA 
ACTAAAGCAA GGACATTTTT TTGGCCACTG GGATCTTCCC AACCTCTTCA CTGGCACTCC 
CTGCTTTCAG TGTTTAATTT AGGCCAGTAT CGACGAGAGG CAGTGAGCTA CAAGAACTAT 
GAATTCTTTC TTCCAGACAA CATGGAAGCC CTGCAAATCA GGAAGCAGTG CGCCCTGGCA 
GCCCTGAAGG ATGTTCACAA CTATCTCAGC CATGAGGAAG GTCATGTTGC GGTTTTTGAT 
GCCACCAACA CTACCAGAGA ACGACGGTCA CTGATCCTGC AGTTTGCAAA AGAACATGGT 
TACAAGGTGT TTTTCATTGA GTCCATTTGT AATGACCCTG GCATAATTGC AGAAAACATC 
AGGCAAGTGA AACTTGGCAG CCCTGATTAT ATAGACTGTG ACCGGGAAAA GGTTCTGGAA 
GACTTTCTAA AGAGAATTGA GTGCTATGAG GTCAACTACC AACCCTTGGA TGAGGAACTG 
GACAGCCACC TGTCCTACAT CAAGATCTTC GACGTGGGCA CACGCTACAT GGTGAACCGA 
GTGCAGGATC ACATCCAGAG CCGCACAGTC TACTACCTCA TGAATATCCA TGTCACACCT 
CGCTCCATCT ACCTTTGCCG ACATGGCGAG AGTGAACTCA ACATCAGAGG CCGCATCGGA 
GGTGACTCTG GCCTCTCAGT TCGCGGCAAG CAGTATGCCT ATGCCCTGGC CAACTTCATT 
CAGTCCCAGG GCATCAGCTC CCTGAAGGTG TGGACCAGTC ACATGAAGAG GACCATCCAG 
ACAGCTGAGG CCCTGGGTGT CCCCTATGAG CAGTGGAAGG CCCTGAATGA GATTGATGCG 
GGTGTCTGTG AGGAGATGAC CTATGAAGAA ATCCAGGAAC ATTACCCTGA AGAATTTGCA 
CTGCGAGACC AAGATAAATA TCGCTACCGC TATCCCAAGG GAGAGTCCTA TGAGGATCTG 
GTTCAGCGTC TGGAGCCAGT GATAATGGAG CTAGAACGAC AGGAGAATGT ACTGGTGATC 
TGCCACCAGG CTGTCATGCG GTGCCTCCTG GCCTATTTCC TGGATAAAAG TTCAGGTGAA 
TGCTGGAACA AGGCCCAGAG AGATGATGTG ACTGAGAATG CAGACCCACA GAACATCAGG 
GCTGGGGCAC CCTCAGAGTA CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885066.1
CDS	389..1795
Translation	MSFGATPKMSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKARTFFWPLGSSQPLHWHSLLSVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSGECWNKAQRDDVTENADPQNIRAGAPSEYI

Target ORF information:

RefSeq Version	XM_017029577.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029577.1

ORF Insert Sequence:

ATGTCATTCG GTGCGACTCC TAAGATGTCT CCAGAGATGG GAGAGCTCAC CCAAACCAGG 
TTGCAGAAGA TCTGGATTCC ACACAGCAGC GGCAGCAGCA GGCTGCAACG GAGAAGGGGC 
TCATCCATAC CCCAGTTTAC CAATTCCCCC ACAATGGTGA TCATGGTGGG TTTACCAGCT 
CGAGGCAAGA CCTATATCTC CACAAAGCTC ACACGATATC TCAACTGGAT AGGAACACCA 
ACTAAAGCAA GGACATTTTT TTGGCCACTG GGATCTTCCC AACCTCTTCA CTGGCACTCC 
CTGCTTTCAG TGTTTAATTT AGGCCAGTAT CGACGAGAGG CAGTGAGCTA CAAGAACTAT 
GAATTCTTTC TTCCAGACAA CATGGAAGCC CTGCAAATCA GGAAGCAGTG CGCCCTGGCA 
GCCCTGAAGG ATGTTCACAA CTATCTCAGC CATGAGGAAG GTCATGTTGC GGTTTTTGAT 
GCCACCAACA CTACCAGAGA ACGACGGTCA CTGATCCTGC AGTTTGCAAA AGAACATGGT 
TACAAGGTGT TTTTCATTGA GTCCATTTGT AATGACCCTG GCATAATTGC AGAAAACATC 
AGGCAAGTGA AACTTGGCAG CCCTGATTAT ATAGACTGTG ACCGGGAAAA GGTTCTGGAA 
GACTTTCTAA AGAGAATTGA GTGCTATGAG GTCAACTACC AACCCTTGGA TGAGGAACTG 
GACAGCCACC TGTCCTACAT CAAGATCTTC GACGTGGGCA CACGCTACAT GGTGAACCGA 
GTGCAGGATC ACATCCAGAG CCGCACAGTC TACTACCTCA TGAATATCCA TGTCACACCT 
CGCTCCATCT ACCTTTGCCG ACATGGCGAG AGTGAACTCA ACATCAGAGG CCGCATCGGA 
GGTGACTCTG GCCTCTCAGT TCGCGGCAAG CAGTATGCCT ATGCCCTGGC CAACTTCATT 
CAGTCCCAGG GCATCAGCTC CCTGAAGGTG TGGACCAGTC ACATGAAGAG GACCATCCAG 
ACAGCTGAGG CCCTGGGTGT CCCCTATGAG CAGTGGAAGG CCCTGAATGA GATTGATGCG 
GGTGTCTGTG AGGAGATGAC CTATGAAGAA ATCCAGGAAC ATTACCCTGA AGAATTTGCA 
CTGCGAGACC AAGATAAATA TCGCTACCGC TATCCCAAGG GAGAGTCCTA TGAGGATCTG 
GTTCAGCGTC TGGAGCCAGT GATAATGGAG CTAGAACGAC AGGAGAATGT ACTGGTGATC 
TGCCACCAGG CTGTCATGCG GTGCCTCCTG GCCTATTTCC TGGATAAAAG TTCAGGTGAA 
TGCTGGAACA AGGCCCAGAG AGATGATGTG ACTGAGAATG CAGACCCACA GAACATCAGG 
GCTGGGGCAC CCTCAGAGTA CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKFB1 ( XM_017029578.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885067.1 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu99335
Clone ID Related Accession (Same CDS sequence)	XM_017029578.1
Accession Version	XM_017029578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGCAAGACCT ATATCTCCAC AAAGCTCACA 
CGATATCTCA ACTGGATAGG AACACCAACT AAAGCAAGGA CATTTTTTTG GCCACTGGGA 
TCTTCCCAAC CTCTTCACTG GCACTCCCTG CTTTCAGTGT TTAATTTAGG CCAGTATCGA 
CGAGAGGCAG TGAGCTACAA GAACTATGAA TTCTTTCTTC CAGACAACAT GGAAGCCCTG 
CAAATCAGGA AGCAGTGCGC CCTGGCAGCC CTGAAGGATG TTCACAACTA TCTCAGCCAT 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGGTCACTG 
ATCCTGCAGT TTGCAAAAGA ACATGGTTAC AAGGTGTTTT TCATTGAGTC CATTTGTAAT 
GACCCTGGCA TAATTGCAGA AAACATCAGG CAAGTGAAAC TTGGCAGCCC TGATTATATA 
GACTGTGACC GGGAAAAGGT TCTGGAAGAC TTTCTAAAGA GAATTGAGTG CTATGAGGTC 
AACTACCAAC CCTTGGATGA GGAACTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT GAACCGAGTG CAGGATCACA TCCAGAGCCG CACAGTCTAC 
TACCTCATGA ATATCCATGT CACACCTCGC TCCATCTACC TTTGCCGACA TGGCGAGAGT 
GAACTCAACA TCAGAGGCCG CATCGGAGGT GACTCTGGCC TCTCAGTTCG CGGCAAGCAG 
TATGCCTATG CCCTGGCCAA CTTCATTCAG TCCCAGGGCA TCAGCTCCCT GAAGGTGTGG 
ACCAGTCACA TGAAGAGGAC CATCCAGACA GCTGAGGCCC TGGGTGTCCC CTATGAGCAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAGG AGATGACCTA TGAAGAAATC 
CAGGAACATT ACCCTGAAGA ATTTGCACTG CGAGACCAAG ATAAATATCG CTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTGG AGCCAGTGAT AATGGAGCTA 
GAACGACAGG AGAATGTACT GGTGATCTGC CACCAGGCTG TCATGCGGTG CCTCCTGGCC 
TATTTCCTGG ATAAAAGTTC AGATGAGCTT CCATATCTCA AGTGCCCTCT GCACACAGTG 
CTCAAACTCA CTCCTGTGGC TTATGGCTGC AAAGTGGAAT CCATCTACCT GAATGTGGAG 
GCCGTGAACA CACACCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAGGAA 
GCCCTGGATA CTGTCCCAGC CCACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885067.1
CDS	624..1973
Translation	MVIMVGLPARGKTYISTKLTRYLNWIGTPTKARTFFWPLGSSQPLHWHSLLSVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY

Target ORF information:

RefSeq Version	XM_017029578.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029578.1

ORF Insert Sequence:

ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGCAAGACCT ATATCTCCAC AAAGCTCACA 
CGATATCTCA ACTGGATAGG AACACCAACT AAAGCAAGGA CATTTTTTTG GCCACTGGGA 
TCTTCCCAAC CTCTTCACTG GCACTCCCTG CTTTCAGTGT TTAATTTAGG CCAGTATCGA 
CGAGAGGCAG TGAGCTACAA GAACTATGAA TTCTTTCTTC CAGACAACAT GGAAGCCCTG 
CAAATCAGGA AGCAGTGCGC CCTGGCAGCC CTGAAGGATG TTCACAACTA TCTCAGCCAT 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGGTCACTG 
ATCCTGCAGT TTGCAAAAGA ACATGGTTAC AAGGTGTTTT TCATTGAGTC CATTTGTAAT 
GACCCTGGCA TAATTGCAGA AAACATCAGG CAAGTGAAAC TTGGCAGCCC TGATTATATA 
GACTGTGACC GGGAAAAGGT TCTGGAAGAC TTTCTAAAGA GAATTGAGTG CTATGAGGTC 
AACTACCAAC CCTTGGATGA GGAACTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT GAACCGAGTG CAGGATCACA TCCAGAGCCG CACAGTCTAC 
TACCTCATGA ATATCCATGT CACACCTCGC TCCATCTACC TTTGCCGACA TGGCGAGAGT 
GAACTCAACA TCAGAGGCCG CATCGGAGGT GACTCTGGCC TCTCAGTTCG CGGCAAGCAG 
TATGCCTATG CCCTGGCCAA CTTCATTCAG TCCCAGGGCA TCAGCTCCCT GAAGGTGTGG 
ACCAGTCACA TGAAGAGGAC CATCCAGACA GCTGAGGCCC TGGGTGTCCC CTATGAGCAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAGG AGATGACCTA TGAAGAAATC 
CAGGAACATT ACCCTGAAGA ATTTGCACTG CGAGACCAAG ATAAATATCG CTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTGG AGCCAGTGAT AATGGAGCTA 
GAACGACAGG AGAATGTACT GGTGATCTGC CACCAGGCTG TCATGCGGTG CCTCCTGGCC 
TATTTCCTGG ATAAAAGTTC AGATGAGCTT CCATATCTCA AGTGCCCTCT GCACACAGTG 
CTCAAACTCA CTCCTGTGGC TTATGGCTGC AAAGTGGAAT CCATCTACCT GAATGTGGAG 
GCCGTGAACA CACACCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAGGAA 
GCCCTGGATA CTGTCCCAGC CCACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKFB1 ( XM_017029576.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885065.1 Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu99333
Clone ID Related Accession (Same CDS sequence)	XM_017029576.1
Accession Version	XM_017029576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATTCG GTGCGACTCC TAAGATGTCT CCAGAGATGG GAGAGCTCAC CCAAACCAGG 
TTGCAGAAGA TCTGGATTCC ACACAGCAGC GGCAGCAGCA GGCTGCAACG GAGAAGGGGC 
TCATCCATAC CCCAGTTTAC CAATTCCCCC ACAATGGTGA TCATGGTGGG TTTACCAGCT 
CGAGGCAAGA CCTATATCTC CACAAAGCTC ACACGATATC TCAACTGGAT AGGAACACCA 
ACTAAAGCAA GGACATTTTT TTGGCCACTG GGATCTTCCC AACCTCTTCA CTGGCACTCC 
CTGCTTTCAG TGTTTAATTT AGGCCAGTAT CGACGAGAGG CAGTGAGCTA CAAGAACTAT 
GAATTCTTTC TTCCAGACAA CATGGAAGCC CTGCAAATCA GGAAGCAGTG CGCCCTGGCA 
GCCCTGAAGG ATGTTCACAA CTATCTCAGC CATGAGGAAG GTCATGTTGC GGTTTTTGAT 
GCCACCAACA CTACCAGAGA ACGACGGTCA CTGATCCTGC AGTTTGCAAA AGAACATGGT 
TACAAGGTGT TTTTCATTGA GTCCATTTGT AATGACCCTG GCATAATTGC AGAAAACATC 
AGGCAAGTGA AACTTGGCAG CCCTGATTAT ATAGACTGTG ACCGGGAAAA GGTTCTGGAA 
GACTTTCTAA AGAGAATTGA GTGCTATGAG GTCAACTACC AACCCTTGGA TGAGGAACTG 
GACAGCCACC TGTCCTACAT CAAGATCTTC GACGTGGGCA CACGCTACAT GGTGAACCGA 
GTGCAGGATC ACATCCAGAG CCGCACAGTC TACTACCTCA TGAATATCCA TGTCACACCT 
CGCTCCATCT ACCTTTGCCG ACATGGCGAG AGTGAACTCA ACATCAGAGG CCGCATCGGA 
GGTGACTCTG GCCTCTCAGT TCGCGGCAAG CAGTATGCCT ATGCCCTGGC CAACTTCATT 
CAGTCCCAGG GCATCAGCTC CCTGAAGGTG TGGACCAGTC ACATGAAGAG GACCATCCAG 
ACAGCTGAGG CCCTGGGTGT CCCCTATGAG CAGTGGAAGG CCCTGAATGA GATTGATGCG 
GGTGTCTGTG AGGAGATGAC CTATGAAGAA ATCCAGGAAC ATTACCCTGA AGAATTTGCA 
CTGCGAGACC AAGATAAATA TCGCTACCGC TATCCCAAGG GAGAGTCCTA TGAGGATCTG 
GTTCAGCGTC TGGAGCCAGT GATAATGGAG CTAGAACGAC AGGAGAATGT ACTGGTGATC 
TGCCACCAGG CTGTCATGCG GTGCCTCCTG GCCTATTTCC TGGATAAAAG TTCAGATGAG 
CTTCCATATC TCAAGTGCCC TCTGCACACA GTGCTCAAAC TCACTCCTGT GGCTTATGGC 
TGCAAAGTGG AATCCATCTA CCTGAATGTG GAGGCCGTGA ACACACACCG GGAGAAGCCT 
GAGAATGTGG ACATCACCCG GGAACCTGAG GAAGCCCTGG ATACTGTCCC AGCCCACTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885065.1
CDS	389..1891
Translation	MSFGATPKMSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKARTFFWPLGSSQPLHWHSLLSVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY

Target ORF information:

RefSeq Version	XM_017029576.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029576.1

ORF Insert Sequence:

ATGTCATTCG GTGCGACTCC TAAGATGTCT CCAGAGATGG GAGAGCTCAC CCAAACCAGG 
TTGCAGAAGA TCTGGATTCC ACACAGCAGC GGCAGCAGCA GGCTGCAACG GAGAAGGGGC 
TCATCCATAC CCCAGTTTAC CAATTCCCCC ACAATGGTGA TCATGGTGGG TTTACCAGCT 
CGAGGCAAGA CCTATATCTC CACAAAGCTC ACACGATATC TCAACTGGAT AGGAACACCA 
ACTAAAGCAA GGACATTTTT TTGGCCACTG GGATCTTCCC AACCTCTTCA CTGGCACTCC 
CTGCTTTCAG TGTTTAATTT AGGCCAGTAT CGACGAGAGG CAGTGAGCTA CAAGAACTAT 
GAATTCTTTC TTCCAGACAA CATGGAAGCC CTGCAAATCA GGAAGCAGTG CGCCCTGGCA 
GCCCTGAAGG ATGTTCACAA CTATCTCAGC CATGAGGAAG GTCATGTTGC GGTTTTTGAT 
GCCACCAACA CTACCAGAGA ACGACGGTCA CTGATCCTGC AGTTTGCAAA AGAACATGGT 
TACAAGGTGT TTTTCATTGA GTCCATTTGT AATGACCCTG GCATAATTGC AGAAAACATC 
AGGCAAGTGA AACTTGGCAG CCCTGATTAT ATAGACTGTG ACCGGGAAAA GGTTCTGGAA 
GACTTTCTAA AGAGAATTGA GTGCTATGAG GTCAACTACC AACCCTTGGA TGAGGAACTG 
GACAGCCACC TGTCCTACAT CAAGATCTTC GACGTGGGCA CACGCTACAT GGTGAACCGA 
GTGCAGGATC ACATCCAGAG CCGCACAGTC TACTACCTCA TGAATATCCA TGTCACACCT 
CGCTCCATCT ACCTTTGCCG ACATGGCGAG AGTGAACTCA ACATCAGAGG CCGCATCGGA 
GGTGACTCTG GCCTCTCAGT TCGCGGCAAG CAGTATGCCT ATGCCCTGGC CAACTTCATT 
CAGTCCCAGG GCATCAGCTC CCTGAAGGTG TGGACCAGTC ACATGAAGAG GACCATCCAG 
ACAGCTGAGG CCCTGGGTGT CCCCTATGAG CAGTGGAAGG CCCTGAATGA GATTGATGCG 
GGTGTCTGTG AGGAGATGAC CTATGAAGAA ATCCAGGAAC ATTACCCTGA AGAATTTGCA 
CTGCGAGACC AAGATAAATA TCGCTACCGC TATCCCAAGG GAGAGTCCTA TGAGGATCTG 
GTTCAGCGTC TGGAGCCAGT GATAATGGAG CTAGAACGAC AGGAGAATGT ACTGGTGATC 
TGCCACCAGG CTGTCATGCG GTGCCTCCTG GCCTATTTCC TGGATAAAAG TTCAGATGAG 
CTTCCATATC TCAAGTGCCC TCTGCACACA GTGCTCAAAC TCACTCCTGT GGCTTATGGC 
TGCAAAGTGG AATCCATCTA CCTGAATGTG GAGGCCGTGA ACACACACCG GGAGAAGCCT 
GAGAATGTGG ACATCACCCG GGAACCTGAG GAAGCCCTGG ATACTGTCCC AGCCCACTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[EXPRESSION OF GENES, WHICH CONTROL GLUCOSE METABOLISM, IN BLOOD CELLS OF THE OBESE BOYS WITH INSULIN RESISTANCE].
Likars'ka sprava(3-4)66-71(2015 Apr-Jun)
Tiazhka OV,Minchenko DO,Davydov VV,Moliavko OS,Budreiko OA,Kulieshova DK,Minchenko OH

Perioperative genomic profiles using structure-specific oligonucleotide probes.
Clinical medicine & research7(3)69-84(2009 Sep)
Hogan KJ,Burmester JK,Caldwell MD,Hogan QH,Coursin DB,Green DN,Selzer RM,Broderick TP,Rusy DA,Poroli M,Lutz AL,Sanders AM,Oldenburg MC,Koelbl JA,de Arruda-Indig M,Halsey JL,Day SP,Domanico MJ

The DNA sequence of the human X chromosome.
Nature434(7031)325-37(2005 Mar)
Ross MT,Grafham DV,Coffey AJ,Scherer S,McLay K,Muzny D,Platzer M,Howell GR,Burrows C,Bird CP,Frankish A,Lovell FL,Howe KL,Ashurst JL,Fulton RS,Sudbrak R,Wen G,Jones MC,Hurles ME,Andrews TD,Scott CE,Searle S,Ramser J,Whittaker A,Deadman R,Carter NP,Hunt SE,Chen R,Cree A,Gunaratne P,Havlak P,Hodgson A,Metzker ML,Richards S,Scott G,Steffen D,Sodergren E,Wheeler DA,Worley KC,Ainscough R,Ambrose KD,Ansari-Lari MA,Aradhya S,Ashwell RI,Babbage AK,Bagguley CL,Ballabio A,Banerjee R,Barker GE,Barlow KF,Barrett IP,Bates KN,Beare DM,Beasley H,Beasley O,Beck A,Bethel G,Blechschmidt K,Brady N,Bray-Allen S,Bridgeman AM,Brown AJ,Brown MJ,Bonnin D,Bruford EA,Buhay C,Burch P,Burford D,Burgess J,Burrill W,Burton J,Bye JM,Carder C,Carrel L,Chako J,Chapman JC,Chavez D,Chen E,Chen G,Chen Y,Chen Z,Chinault C,Ciccodicola A,Clark SY,Clarke G,Clee CM,Clegg S,Clerc-Blankenburg K,Clifford K,Cobley V,Cole CG,Conquer JS,Corby N,Connor RE,David R,Davies J,Davis C,Davis J,Delgado O,Deshazo D,Dhami P,Ding Y,Dinh H,Dodsworth S,Draper H,Dugan-Rocha S,Dunham A,Dunn M,Durbin KJ,Dutta I,Eades T,Ellwood M,Emery-Cohen A,Errington H,Evans KL,Faulkner L,Francis F,Frankland J,Fraser AE,Galgoczy P,Gilbert J,Gill R,Gl?ckner G,Gregory SG,Gribble S,Griffiths C,Grocock R,Gu Y,Gwilliam R,Hamilton C,Hart EA,Hawes A,Heath PD,Heitmann K,Hennig S,Hernandez J,Hinzmann B,Ho S,Hoffs M,Howden PJ,Huckle EJ,Hume J,Hunt PJ,Hunt AR,Isherwood J,Jacob L,Johnson D,Jones S,de Jong PJ,Joseph SS,Keenan S,Kelly S,Kershaw JK,Khan Z,Kioschis P,Klages S,Knights AJ,Kosiura A,Kovar-Smith C,Laird GK,Langford C,Lawlor S,Leversha M,Lewis L,Liu W,Lloyd C,Lloyd DM,Loulseged H,Loveland JE,Lovell JD,Lozado R,Lu J,Lyne R,Ma J,Maheshwari M,Matthews LH,McDowall J,McLaren S,McMurray A,Meidl P,Meitinger T,Milne S,Miner G,Mistry SL,Morgan M,Morris S,M?ller I,Mullikin JC,Nguyen N,Nordsiek G,Nyakatura G,O'Dell CN,Okwuonu G,Palmer S,Pandian R,Parker D,Parrish J,Pasternak S,Patel D,Pearce AV,Pearson DM,Pelan SE,Perez L,Porter KM,Ramsey Y,Reichwald K,Rhodes S,Ridler KA,Schlessinger D,Schueler MG,Sehra HK,Shaw-Smith C,Shen H,Sheridan EM,Shownkeen R,Skuce CD,Smith ML,Sotheran EC,Steingruber HE,Steward CA,Storey R,Swann RM,Swarbreck D,Tabor PE,Taudien S,Taylor T,Teague B,Thomas K,Thorpe A,Timms K,Tracey A,Trevanion S,Tromans AC,d'Urso M,Verduzco D,Villasana D,Waldron L,Wall M,Wang Q,Warren J,Warry GL,Wei X,West A,Whitehead SL,Whiteley MN,Wilkinson JE,Willey DL,Williams G,Williams L,Williamson A,Williamson H,Wilming L,Woodmansey RL,Wray PW,Yen J,Zhang J,Zhou J,Zoghbi H,Zorilla S,Buck D,Reinhardt R,Poustka A,Rosenthal A,Lehrach H,Meindl A,Minx PJ,Hillier LW,Willard HF,Wilson RK,Waterston RH,Rice CM,Vaudin M,Coulson A,Nelson DL,Weinstock G,Sulston JE,Durbin R,Hubbard T,Gibbs RA,Beck S,Rogers J,Bentley DR

A humble hexose monophosphate pathway metabolite regulates short- and long-term control of lipogenesis.
Proceedings of the National Academy of Sciences of the United States of America100(10)5578-80(2003 May)
Veech RL

Tissue-specific structure/function differentiation of the liver isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
The Journal of biological chemistry278(1)523-30(2003 Jan)
Lee YH,Li Y,Uyeda K,Hasemann CA

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オンライン注文

PFKFB1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム