PFAS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PFAS
Entrez Gene ID	5198
Full Name	phosphoribosylformylglycinamidine synthase
Synonyms	FGAMS,FGAR-AT,FGARAT,GATD8,PURL
General protein information	Preferred Names phosphoribosylformylglycinamidine synthase Names phosphoribosylformylglycinamidine synthase FGAM synthase FGAR amidotransferase formylglycinamide ribonucleotide amidotransferase formylglycinamide ribotide amidotransferase formylglycinamide ribotide synthetase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.1
Summary	Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. The enzyme encoded by this gene catalyzes the fourth step of IMP biosynthesis. [provided by RefSeq, Jul 2008].
Disorder MIM:	602133

mRNA and Protein(s)

mRNA	Protein	Name
XM_024450804.1	XP_024306572.1	phosphoribosylformylglycinamidine synthase isoform X1
XM_006721546.3	XP_006721609.1	phosphoribosylformylglycinamidine synthase isoform X3
NM_012393.3	NP_036525.1	phosphoribosylformylglycinamidine synthase
XM_006721546.2	XP_006721609.1	phosphoribosylformylglycinamidine synthase isoform X2
NM_012393.2	NP_036525.1	phosphoribosylformylglycinamidine synthase
XM_017024760.1	XP_016880249.1	phosphoribosylformylglycinamidine synthase isoform X2

Pathways from BioSystems

Homologies

	PFAS	XP_001112405.1
Pan troglodytes (chimpanzee)	PFAS	XP_511854.3
Bos taurus (cattle)	PFAS	NP_001243493.1
Mus musculus (house mouse)	Pfas	NP_001152991.1
Drosophila melanogaster (fruit fly)	ade2	NP_723146.1
Caenorhabditis elegans (roundworm)	CELE_F10F2.2	NP_497942.2
Homo sapiens (human)	PFAS	NP_036525.1
Canis lupus familiaris (dog)	PFAS	XP_005620078.1
Rattus norvegicus (Norway rat)	Pfas	NP_001099261.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002091	XP_320948.2
Arabidopsis thaliana (thale cress)	PUR4	NP_177566.3
Danio rerio (zebrafish)	pfas	NP_001038667.1
Oryza sativa (rice)	Os01g0888500	NP_001045039.1
Xenopus tropicalis (tropical clawed frog)	pfas	NP_001135692.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression of the purine biosynthetic enzyme phosphoribosyl formylglycinamidine synthase (FGAMS) in neurons.
Mangold CA, Yao PJ, Du M, Freeman WM, Benkovic SJ, Szpara ML
Journal of neurochemistry(2018 Jan)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Girard A, Sachidanandam R, Hannon GJ, Carmell MA
Nature442(7099)199-202(2006 Jul)

Human phosphoribosylformylglycineamide amidotransferase (FGARAT): regional mapping, complete coding sequence, isolation of a functional genomic clone, and DNA sequence analysis.
Patterson D, Bleskan J, Gardiner K, Bowersox J
Gene239(2)381-91(1999 Nov)

The human GARS-AIRS-GART gene encodes two proteins which are differentially expressed during human brain development and temporally overexpressed in cerebellum of individuals with Down syndrome.
Brodsky G, Barnes T, Bleskan J, Becker L, Cox M, Patterson D
Human molecular genetics6(12)2043-50(1997 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following PFAS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFAS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu49877	XM_006721546.3 Latest version!	Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu59620	XM_017024760.1 Latest version!	Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OHu31922	NM_012393.3 Latest version!	Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
OHu31922	XM_024450804.1 Latest version!	Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
View Old Accession Versions >>
OHu49877	XM_006721546.2	Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu31922	NM_012393.2	Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$384.30 $549.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PFAS ( XM_006721546.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006721609.1 Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu49877
Clone ID Related Accession (Same CDS sequence)	XM_006721546.3 , XM_006721546.2
Accession Version	XM_006721546.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phosphoribosylformylglycinamidine synthase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006721546.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCCAG TCCTTCACTT CTATGTTCGT CCCTCTGGCC ATGAGGGGGC AGCCCCTGGA 
CACACTCGGA GGAAACTGCA AGGGAAACTG CCAGAGCTGC AGGGCGTCGA GACTGAACTG 
TGCTACAACG TGAACTGGAC AGCTGAGGCC CTCCCCAGTG CTGAGGAGAC AAAGAAGCTG 
ATGTGGCTGT TTGGTTGCCC CTTACTGCTG GATGATGTTG CTCGGGAGTC CTGGCTCCTT 
CCTGGCTCCA ATGACCTGCT GCTGGAGGTC GGGCCCAGGC TGAACTTCTC CACCCCAACA 
TCCACCAACA TCGTGTCAGT GTGCCGCGCC ACTGGGCTGG GGCCTGTGGA TCGTGTGGAG 
ACCACCCGGC GCTACCGGCT CTCGTTTGCC CACCCCCCGT CAGCTGAGGT GGAAGCCATT 
GCTCTGGCTA CCCTGCACGA CCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGT 
TTCTCCCCTG AGAGCATGCC GGAACCCCTC AATGGCCCTA TCAATATACT GGGTGAGGGC 
CGGCTTGCGC TGGAGAAGGC CAACCAGGAG CTTGGTCTGG CTTTAGACTC TTGGGACCTA 
GACTTCTACA CCAAGCGCTT CCAGGAGCTA CAGCGGAACC CGAGCACTGT GGAGGCCTTT 
GACTTGGCGC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
GTGGATGGGC AGAAGCTGGT GCACTCACTG TTTGAGTCCA TCATGAGCAC CCAGGAATCC 
TCGAACCCCA ACAACGTCCT CAAATTCTGT GATAACAGCA GTGCAATCCA GGGAAAGGAA 
GTCCGATTCC TACGGCCTGA GGACCCCACA CGGCCAAGCC GCTTCCAGCA ACAGCAAGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACT CACAACTTTC CCACAGGAGT ATGCCCCTTT 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATTCGAGATG TCCAGTGCAC AGGCCGCGGG 
GCCCACGTGG TGGCTGGCAC TGCCGGCTAT TGCTTTGGAA ATCTGCATAT TCCAGGTTAC 
AATCTGCCCT GGGAGGATCC AAGCTTCCAG TATCCTGGGA ATTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AAGCCAGTAA TGGAGCTTCT GACTATGGCA ACAAGTTTGG GGAACCAGTG 
CTGGCTGGCT TCGCCCGCTC CTTGGGCCTC CAGCTCCCAG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGGGGCATT GGGTCCATGG AAGCTGACCA CATAAGCAAG 
GAGGCCCCAG AGCCAGGCAT GGAAGTTGTA AAGGTTGGAG GTCCCGTCTA CAGGATTGGA 
GTTGGAGGTG GAGCTGCTTC ATCTGTGCAG GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGAGG AGACCCGGAG ATGGAACAGA AGATGAACCG TGTGATCAGG 
GCTTGTGTGG AGGCCCCCAA GGGAAACCCC ATCTGCAGCC TTCATGATCA GGGCGCTGGT 
GGCAATGGCA ATGTCCTAAA AGAGCTGAGT GACCCAGCTG GAGCCATCAT TTACACCAGC 
CGCTTCCAGC TTGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC TGAGTACCAG 
GAATCAAATG CTCTTCTGCT GAGGTCCCCC AACCGGGACT TCCTGACTCA TGTCAGTGCC 
CGTGAACGTT GCCCGGCTTG CTTCGTGGGC ACCATCACTG GAGACCGGAG AATAGTGCTG 
GTGGACGATC GGGAGTGTCC TGTCAGAAGA AATGGCCAGG GGGATGCCCC CCCGACACCC 
CTGCCAACCC CTGTGGACCT GGAGCTCGAA TGGGTGCTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTGC AGAGGAAGCC CCCCATGCTG CAGCCTCTGG CCTTGCCCCC AGGGCTGAGC 
GTGCACCAGG CTCTGGAGAG GGTTCTGAGG CTGCCCGCCG TGGCCAGCAA GCGCTACCTC 
ACCAATAAGG TGGACCGCTC TGTGGGAGGC CTGGTGGCCC AGCAGCAGTG CGTGGGGCCC 
CTGCAAACTC CTCTGGCAGA TGTAGCGGTT GTGGCACTGA GCCATGAGGA GCTCATAGGG 
GCTGCCACAG CCTTGGGAGA ACAGCCAGTC AAGAGCCTGC TGGACCCAAA AGTCGCCGCC 
CGGCTGGCCG TGGCCGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC TGACCTCCGG 
GATGTGAAGT GTAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGCGCAGCT 
TTGGCGGATG CCTGTGAGGC TATGGTGGCA GTGATGGCAG CCCTGGGTGT GGCAGTGGAT 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCT CGGGTTGGCA CTGAGACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCCGTC TGCCCAGACA TCACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGAAGG GAGAGGCCAT CTGCTCTATG TGGCTCTGAG CCCTGGGCAG 
CACCGGCTCG GGGGCACAGC TCTGGCCCAG TGCTTCTCCC AGCTTGGGGA ACACCCTCCA 
GACCTGGACC TTCCTGAGAA CTTGGTGCGG GCCTTCAGCA TCACTCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCACGATGTC AGTGACGGAG GCCTCGTCAC ATGCCTGCTG 
GAGATGGCCT TTGCTGGAAA TTGCGGGCTA CAGGTGGATG TGCCTGTCCC CAGGGTTGAT 
GCTGAACTGG ATGGAACTGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006721609.1
CDS	139..2982
Translation	MSPVLHFYVRPSGHEGAAPGHTRRKLQGKLPELQGVETELCYNVNWTAEALPSAEETKKLMWLFGCPLLLDDVARESWLLPGSNDLLLEVGPRLNFSTPTSTNIVSVCRATGLGPVDRVETTRRYRLSFAHPPSAEVEAIALATLHDRMTEQHFPHPIQSFSPESMPEPLNGPINILGEGRLALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHVDGQKLVHSLFESIMSTQESSNPNNVLKFCDNSSAIQGKEVRFLRPEDPTRPSRFQQQQGLRHVVFTAETHNFPTGVCPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPGNFARPLEVAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEADHISKEAPEPGMEVVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAPKGNPICSLHDQGAGGNGNVLKELSDPAGAIIYTSRFQLGDPTLNALEIWGAEYQESNALLLRSPNRDFLTHVSARERCPACFVGTITGDRRIVLVDDRECPVRRNGQGDAPPTPLPTPVDLELEWVLGKMPRKEFFLQRKPPMLQPLALPPGLSVHQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHEELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGTETVRAPGSLVISAYAVCPDITATVTPDLKHPEGRGHLLYVALSPGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFSITQGLLKDRLLCSGHDVSDGGLVTCLLEMAFAGNCGLQVDVPVPRVDAELDGTG

Target ORF information:

RefSeq Version	XM_006721546.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721546.3

ORF Insert Sequence:

ATGTCCCCAG TCCTTCACTT CTATGTTCGT CCCTCTGGCC ATGAGGGGGC AGCCCCTGGA 
CACACTCGGA GGAAACTGCA AGGGAAACTG CCAGAGCTGC AGGGCGTCGA GACTGAACTG 
TGCTACAACG TGAACTGGAC AGCTGAGGCC CTCCCCAGTG CTGAGGAGAC AAAGAAGCTG 
ATGTGGCTGT TTGGTTGCCC CTTACTGCTG GATGATGTTG CTCGGGAGTC CTGGCTCCTT 
CCTGGCTCCA ATGACCTGCT GCTGGAGGTC GGGCCCAGGC TGAACTTCTC CACCCCAACA 
TCCACCAACA TCGTGTCAGT GTGCCGCGCC ACTGGGCTGG GGCCTGTGGA TCGTGTGGAG 
ACCACCCGGC GCTACCGGCT CTCGTTTGCC CACCCCCCGT CAGCTGAGGT GGAAGCCATT 
GCTCTGGCTA CCCTGCACGA CCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGT 
TTCTCCCCTG AGAGCATGCC GGAACCCCTC AATGGCCCTA TCAATATACT GGGTGAGGGC 
CGGCTTGCGC TGGAGAAGGC CAACCAGGAG CTTGGTCTGG CTTTAGACTC TTGGGACCTA 
GACTTCTACA CCAAGCGCTT CCAGGAGCTA CAGCGGAACC CGAGCACTGT GGAGGCCTTT 
GACTTGGCGC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
GTGGATGGGC AGAAGCTGGT GCACTCACTG TTTGAGTCCA TCATGAGCAC CCAGGAATCC 
TCGAACCCCA ACAACGTCCT CAAATTCTGT GATAACAGCA GTGCAATCCA GGGAAAGGAA 
GTCCGATTCC TACGGCCTGA GGACCCCACA CGGCCAAGCC GCTTCCAGCA ACAGCAAGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACT CACAACTTTC CCACAGGAGT ATGCCCCTTT 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATTCGAGATG TCCAGTGCAC AGGCCGCGGG 
GCCCACGTGG TGGCTGGCAC TGCCGGCTAT TGCTTTGGAA ATCTGCATAT TCCAGGTTAC 
AATCTGCCCT GGGAGGATCC AAGCTTCCAG TATCCTGGGA ATTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AAGCCAGTAA TGGAGCTTCT GACTATGGCA ACAAGTTTGG GGAACCAGTG 
CTGGCTGGCT TCGCCCGCTC CTTGGGCCTC CAGCTCCCAG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGGGGCATT GGGTCCATGG AAGCTGACCA CATAAGCAAG 
GAGGCCCCAG AGCCAGGCAT GGAAGTTGTA AAGGTTGGAG GTCCCGTCTA CAGGATTGGA 
GTTGGAGGTG GAGCTGCTTC ATCTGTGCAG GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGAGG AGACCCGGAG ATGGAACAGA AGATGAACCG TGTGATCAGG 
GCTTGTGTGG AGGCCCCCAA GGGAAACCCC ATCTGCAGCC TTCATGATCA GGGCGCTGGT 
GGCAATGGCA ATGTCCTAAA AGAGCTGAGT GACCCAGCTG GAGCCATCAT TTACACCAGC 
CGCTTCCAGC TTGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC TGAGTACCAG 
GAATCAAATG CTCTTCTGCT GAGGTCCCCC AACCGGGACT TCCTGACTCA TGTCAGTGCC 
CGTGAACGTT GCCCGGCTTG CTTCGTGGGC ACCATCACTG GAGACCGGAG AATAGTGCTG 
GTGGACGATC GGGAGTGTCC TGTCAGAAGA AATGGCCAGG GGGATGCCCC CCCGACACCC 
CTGCCAACCC CTGTGGACCT GGAGCTCGAA TGGGTGCTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTGC AGAGGAAGCC CCCCATGCTG CAGCCTCTGG CCTTGCCCCC AGGGCTGAGC 
GTGCACCAGG CTCTGGAGAG GGTTCTGAGG CTGCCCGCCG TGGCCAGCAA GCGCTACCTC 
ACCAATAAGG TGGACCGCTC TGTGGGAGGC CTGGTGGCCC AGCAGCAGTG CGTGGGGCCC 
CTGCAAACTC CTCTGGCAGA TGTAGCGGTT GTGGCACTGA GCCATGAGGA GCTCATAGGG 
GCTGCCACAG CCTTGGGAGA ACAGCCAGTC AAGAGCCTGC TGGACCCAAA AGTCGCCGCC 
CGGCTGGCCG TGGCCGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC TGACCTCCGG 
GATGTGAAGT GTAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGCGCAGCT 
TTGGCGGATG CCTGTGAGGC TATGGTGGCA GTGATGGCAG CCCTGGGTGT GGCAGTGGAT 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCT CGGGTTGGCA CTGAGACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCCGTC TGCCCAGACA TCACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGAAGG GAGAGGCCAT CTGCTCTATG TGGCTCTGAG CCCTGGGCAG 
CACCGGCTCG GGGGCACAGC TCTGGCCCAG TGCTTCTCCC AGCTTGGGGA ACACCCTCCA 
GACCTGGACC TTCCTGAGAA CTTGGTGCGG GCCTTCAGCA TCACTCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCACGATGTC AGTGACGGAG GCCTCGTCAC ATGCCTGCTG 
GAGATGGCCT TTGCTGGAAA TTGCGGGCTA CAGGTGGATG TGCCTGTCCC CAGGGTTGAT 
GCTGAACTGG ATGGAACTGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_006721546.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006721609.1 Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu49877
Clone ID Related Accession (Same CDS sequence)	XM_006721546.3 , XM_006721546.2
Accession Version	XM_006721546.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	phosphoribosylformylglycinamidine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006721546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCCAG TCCTTCACTT CTATGTTCGT CCCTCTGGCC ATGAGGGGGC AGCCCCTGGA 
CACACTCGGA GGAAACTGCA AGGGAAACTG CCAGAGCTGC AGGGCGTCGA GACTGAACTG 
TGCTACAACG TGAACTGGAC AGCTGAGGCC CTCCCCAGTG CTGAGGAGAC AAAGAAGCTG 
ATGTGGCTGT TTGGTTGCCC CTTACTGCTG GATGATGTTG CTCGGGAGTC CTGGCTCCTT 
CCTGGCTCCA ATGACCTGCT GCTGGAGGTC GGGCCCAGGC TGAACTTCTC CACCCCAACA 
TCCACCAACA TCGTGTCAGT GTGCCGCGCC ACTGGGCTGG GGCCTGTGGA TCGTGTGGAG 
ACCACCCGGC GCTACCGGCT CTCGTTTGCC CACCCCCCGT CAGCTGAGGT GGAAGCCATT 
GCTCTGGCTA CCCTGCACGA CCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGT 
TTCTCCCCTG AGAGCATGCC GGAACCCCTC AATGGCCCTA TCAATATACT GGGTGAGGGC 
CGGCTTGCGC TGGAGAAGGC CAACCAGGAG CTTGGTCTGG CTTTAGACTC TTGGGACCTA 
GACTTCTACA CCAAGCGCTT CCAGGAGCTA CAGCGGAACC CGAGCACTGT GGAGGCCTTT 
GACTTGGCGC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
GTGGATGGGC AGAAGCTGGT GCACTCACTG TTTGAGTCCA TCATGAGCAC CCAGGAATCC 
TCGAACCCCA ACAACGTCCT CAAATTCTGT GATAACAGCA GTGCAATCCA GGGAAAGGAA 
GTCCGATTCC TACGGCCTGA GGACCCCACA CGGCCAAGCC GCTTCCAGCA ACAGCAAGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACT CACAACTTTC CCACAGGAGT ATGCCCCTTT 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATTCGAGATG TCCAGTGCAC AGGCCGCGGG 
GCCCACGTGG TGGCTGGCAC TGCCGGCTAT TGCTTTGGAA ATCTGCATAT TCCAGGTTAC 
AATCTGCCCT GGGAGGATCC AAGCTTCCAG TATCCTGGGA ATTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AAGCCAGTAA TGGAGCTTCT GACTATGGCA ACAAGTTTGG GGAACCAGTG 
CTGGCTGGCT TCGCCCGCTC CTTGGGCCTC CAGCTCCCAG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGGGGCATT GGGTCCATGG AAGCTGACCA CATAAGCAAG 
GAGGCCCCAG AGCCAGGCAT GGAAGTTGTA AAGGTTGGAG GTCCCGTCTA CAGGATTGGA 
GTTGGAGGTG GAGCTGCTTC ATCTGTGCAG GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGAGG AGACCCGGAG ATGGAACAGA AGATGAACCG TGTGATCAGG 
GCTTGTGTGG AGGCCCCCAA GGGAAACCCC ATCTGCAGCC TTCATGATCA GGGCGCTGGT 
GGCAATGGCA ATGTCCTAAA AGAGCTGAGT GACCCAGCTG GAGCCATCAT TTACACCAGC 
CGCTTCCAGC TTGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC TGAGTACCAG 
GAATCAAATG CTCTTCTGCT GAGGTCCCCC AACCGGGACT TCCTGACTCA TGTCAGTGCC 
CGTGAACGTT GCCCGGCTTG CTTCGTGGGC ACCATCACTG GAGACCGGAG AATAGTGCTG 
GTGGACGATC GGGAGTGTCC TGTCAGAAGA AATGGCCAGG GGGATGCCCC CCCGACACCC 
CTGCCAACCC CTGTGGACCT GGAGCTCGAA TGGGTGCTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTGC AGAGGAAGCC CCCCATGCTG CAGCCTCTGG CCTTGCCCCC AGGGCTGAGC 
GTGCACCAGG CTCTGGAGAG GGTTCTGAGG CTGCCCGCCG TGGCCAGCAA GCGCTACCTC 
ACCAATAAGG TGGACCGCTC TGTGGGAGGC CTGGTGGCCC AGCAGCAGTG CGTGGGGCCC 
CTGCAAACTC CTCTGGCAGA TGTAGCGGTT GTGGCACTGA GCCATGAGGA GCTCATAGGG 
GCTGCCACAG CCTTGGGAGA ACAGCCAGTC AAGAGCCTGC TGGACCCAAA AGTCGCCGCC 
CGGCTGGCCG TGGCCGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC TGACCTCCGG 
GATGTGAAGT GTAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGCGCAGCT 
TTGGCGGATG CCTGTGAGGC TATGGTGGCA GTGATGGCAG CCCTGGGTGT GGCAGTGGAT 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCT CGGGTTGGCA CTGAGACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCCGTC TGCCCAGACA TCACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGAAGG GAGAGGCCAT CTGCTCTATG TGGCTCTGAG CCCTGGGCAG 
CACCGGCTCG GGGGCACAGC TCTGGCCCAG TGCTTCTCCC AGCTTGGGGA ACACCCTCCA 
GACCTGGACC TTCCTGAGAA CTTGGTGCGG GCCTTCAGCA TCACTCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCACGATGTC AGTGACGGAG GCCTCGTCAC ATGCCTGCTG 
GAGATGGCCT TTGCTGGAAA TTGCGGGCTA CAGGTGGATG TGCCTGTCCC CAGGGTTGAT 
GCTGAACTGG ATGGAACTGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006721609.1
CDS	165..3008
Translation	MSPVLHFYVRPSGHEGAAPGHTRRKLQGKLPELQGVETELCYNVNWTAEALPSAEETKKLMWLFGCPLLLDDVARESWLLPGSNDLLLEVGPRLNFSTPTSTNIVSVCRATGLGPVDRVETTRRYRLSFAHPPSAEVEAIALATLHDRMTEQHFPHPIQSFSPESMPEPLNGPINILGEGRLALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHVDGQKLVHSLFESIMSTQESSNPNNVLKFCDNSSAIQGKEVRFLRPEDPTRPSRFQQQQGLRHVVFTAETHNFPTGVCPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPGNFARPLEVAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEADHISKEAPEPGMEVVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAPKGNPICSLHDQGAGGNGNVLKELSDPAGAIIYTSRFQLGDPTLNALEIWGAEYQESNALLLRSPNRDFLTHVSARERCPACFVGTITGDRRIVLVDDRECPVRRNGQGDAPPTPLPTPVDLELEWVLGKMPRKEFFLQRKPPMLQPLALPPGLSVHQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHEELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGTETVRAPGSLVISAYAVCPDITATVTPDLKHPEGRGHLLYVALSPGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFSITQGLLKDRLLCSGHDVSDGGLVTCLLEMAFAGNCGLQVDVPVPRVDAELDGTG

Target ORF information:

RefSeq Version	XM_006721546.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721546.2

ORF Insert Sequence:

ATGTCCCCAG TCCTTCACTT CTATGTTCGT CCCTCTGGCC ATGAGGGGGC AGCCCCTGGA 
CACACTCGGA GGAAACTGCA AGGGAAACTG CCAGAGCTGC AGGGCGTCGA GACTGAACTG 
TGCTACAACG TGAACTGGAC AGCTGAGGCC CTCCCCAGTG CTGAGGAGAC AAAGAAGCTG 
ATGTGGCTGT TTGGTTGCCC CTTACTGCTG GATGATGTTG CTCGGGAGTC CTGGCTCCTT 
CCTGGCTCCA ATGACCTGCT GCTGGAGGTC GGGCCCAGGC TGAACTTCTC CACCCCAACA 
TCCACCAACA TCGTGTCAGT GTGCCGCGCC ACTGGGCTGG GGCCTGTGGA TCGTGTGGAG 
ACCACCCGGC GCTACCGGCT CTCGTTTGCC CACCCCCCGT CAGCTGAGGT GGAAGCCATT 
GCTCTGGCTA CCCTGCACGA CCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGT 
TTCTCCCCTG AGAGCATGCC GGAACCCCTC AATGGCCCTA TCAATATACT GGGTGAGGGC 
CGGCTTGCGC TGGAGAAGGC CAACCAGGAG CTTGGTCTGG CTTTAGACTC TTGGGACCTA 
GACTTCTACA CCAAGCGCTT CCAGGAGCTA CAGCGGAACC CGAGCACTGT GGAGGCCTTT 
GACTTGGCGC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
GTGGATGGGC AGAAGCTGGT GCACTCACTG TTTGAGTCCA TCATGAGCAC CCAGGAATCC 
TCGAACCCCA ACAACGTCCT CAAATTCTGT GATAACAGCA GTGCAATCCA GGGAAAGGAA 
GTCCGATTCC TACGGCCTGA GGACCCCACA CGGCCAAGCC GCTTCCAGCA ACAGCAAGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACT CACAACTTTC CCACAGGAGT ATGCCCCTTT 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATTCGAGATG TCCAGTGCAC AGGCCGCGGG 
GCCCACGTGG TGGCTGGCAC TGCCGGCTAT TGCTTTGGAA ATCTGCATAT TCCAGGTTAC 
AATCTGCCCT GGGAGGATCC AAGCTTCCAG TATCCTGGGA ATTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AAGCCAGTAA TGGAGCTTCT GACTATGGCA ACAAGTTTGG GGAACCAGTG 
CTGGCTGGCT TCGCCCGCTC CTTGGGCCTC CAGCTCCCAG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGGGGCATT GGGTCCATGG AAGCTGACCA CATAAGCAAG 
GAGGCCCCAG AGCCAGGCAT GGAAGTTGTA AAGGTTGGAG GTCCCGTCTA CAGGATTGGA 
GTTGGAGGTG GAGCTGCTTC ATCTGTGCAG GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGAGG AGACCCGGAG ATGGAACAGA AGATGAACCG TGTGATCAGG 
GCTTGTGTGG AGGCCCCCAA GGGAAACCCC ATCTGCAGCC TTCATGATCA GGGCGCTGGT 
GGCAATGGCA ATGTCCTAAA AGAGCTGAGT GACCCAGCTG GAGCCATCAT TTACACCAGC 
CGCTTCCAGC TTGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC TGAGTACCAG 
GAATCAAATG CTCTTCTGCT GAGGTCCCCC AACCGGGACT TCCTGACTCA TGTCAGTGCC 
CGTGAACGTT GCCCGGCTTG CTTCGTGGGC ACCATCACTG GAGACCGGAG AATAGTGCTG 
GTGGACGATC GGGAGTGTCC TGTCAGAAGA AATGGCCAGG GGGATGCCCC CCCGACACCC 
CTGCCAACCC CTGTGGACCT GGAGCTCGAA TGGGTGCTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTGC AGAGGAAGCC CCCCATGCTG CAGCCTCTGG CCTTGCCCCC AGGGCTGAGC 
GTGCACCAGG CTCTGGAGAG GGTTCTGAGG CTGCCCGCCG TGGCCAGCAA GCGCTACCTC 
ACCAATAAGG TGGACCGCTC TGTGGGAGGC CTGGTGGCCC AGCAGCAGTG CGTGGGGCCC 
CTGCAAACTC CTCTGGCAGA TGTAGCGGTT GTGGCACTGA GCCATGAGGA GCTCATAGGG 
GCTGCCACAG CCTTGGGAGA ACAGCCAGTC AAGAGCCTGC TGGACCCAAA AGTCGCCGCC 
CGGCTGGCCG TGGCCGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC TGACCTCCGG 
GATGTGAAGT GTAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGCGCAGCT 
TTGGCGGATG CCTGTGAGGC TATGGTGGCA GTGATGGCAG CCCTGGGTGT GGCAGTGGAT 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCT CGGGTTGGCA CTGAGACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCCGTC TGCCCAGACA TCACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGAAGG GAGAGGCCAT CTGCTCTATG TGGCTCTGAG CCCTGGGCAG 
CACCGGCTCG GGGGCACAGC TCTGGCCCAG TGCTTCTCCC AGCTTGGGGA ACACCCTCCA 
GACCTGGACC TTCCTGAGAA CTTGGTGCGG GCCTTCAGCA TCACTCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCACGATGTC AGTGACGGAG GCCTCGTCAC ATGCCTGCTG 
GAGATGGCCT TTGCTGGAAA TTGCGGGCTA CAGGTGGATG TGCCTGTCCC CAGGGTTGAT 
GCTGAACTGG ATGGAACTGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_017024760.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880249.1 Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

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CloneID	OHu59620
Clone ID Related Accession (Same CDS sequence)	XM_017024760.1
Accession Version	XM_017024760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phosphoribosylformylglycinamidine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCC AGGAATCCTC GAACCCCAAC AACGTCCTCA AATTCTGTGA TAACAGCAGT 
GCAATCCAGG GAAAGGAAGT CCGATTCCTA CGGCCTGAGG ACCCCACACG GCCAAGCCGC 
TTCCAGCAAC AGCAAGGGCT GAGACATGTT GTCTTCACAG CAGAGACTCA CAACTTTCCC 
ACAGGAGTAT GCCCCTTTAG TGGTGCAACC ACTGGCACAG GGGGCCGGAT TCGAGATGTC 
CAGTGCACAG GCCGCGGGGC CCACGTGGTG GCTGGCACTG CCGGCTATTG CTTTGGAAAT 
CTGCATATTC CAGGTTACAA TCTGCCCTGG GAGGATCCAA GCTTCCAGTA TCCTGGGAAT 
TTTGCCCGGC CCCTGGAGGT TGCCATTGAA GCCAGTAATG GAGCTTCTGA CTATGGCAAC 
AAGTTTGGGG AACCAGTGCT GGCTGGCTTC GCCCGCTCCT TGGGCCTCCA GCTCCCAGAC 
GGCCAGCGGC GTGAGTGGAT CAAGCCCATC ATGTTTAGTG GGGGCATTGG GTCCATGGAA 
GCTGACCACA TAAGCAAGGA GGCCCCAGAG CCAGGCATGG AAGTTGTAAA GGTTGGAGGT 
CCCGTCTACA GGATTGGAGT TGGAGGTGGA GCTGCTTCAT CTGTGCAGGT GCAGGGAGAT 
AACACCAGTG ACCTGGACTT TGGGGCTGTG CAGCGAGGAG ACCCGGAGAT GGAACAGAAG 
ATGAACCGTG TGATCAGGGC TTGTGTGGAG GCCCCCAAGG GAAACCCCAT CTGCAGCCTT 
CATGATCAGG GCGCTGGTGG CAATGGCAAT GTCCTAAAAG AGCTGAGTGA CCCAGCTGGA 
GCCATCATTT ACACCAGCCG CTTCCAGCTT GGGGACCCAA CCCTGAATGC CCTGGAAATC 
TGGGGGGCTG AGTACCAGGA ATCAAATGCT CTTCTGCTGA GGTCCCCCAA CCGGGACTTC 
CTGACTCATG TCAGTGCCCG TGAACGTTGC CCGGCTTGCT TCGTGGGCAC CATCACTGGA 
GACCGGAGAA TAGTGCTGGT GGACGATCGG GAGTGTCCTG TCAGAAGAAA TGGCCAGGGG 
GATGCCCCCC CGACACCCCT GCCAACCCCT GTGGACCTGG AGCTCGAATG GGTGCTGGGC 
AAGATGCCTC GGAAGGAGTT CTTCCTGCAG AGGAAGCCCC CCATGCTGCA GCCTCTGGCC 
TTGCCCCCAG GGCTGAGCGT GCACCAGGCT CTGGAGAGGG TTCTGAGGCT GCCCGCCGTG 
GCCAGCAAGC GCTACCTCAC CAATAAGGTG GACCGCTCTG TGGGAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCCCT GCAAACTCCT CTGGCAGATG TAGCGGTTGT GGCACTGAGC 
CATGAGGAGC TCATAGGGGC TGCCACAGCC TTGGGAGAAC AGCCAGTCAA GAGCCTGCTG 
GACCCAAAAG TCGCCGCCCG GCTGGCCGTG GCCGAAGCCC TCACCAACCT GGTGTTTGCT 
CTGGTCACTG ACCTCCGGGA TGTGAAGTGT AGCGGGAACT GGATGTGGGC AGCCAAGCTC 
CCAGGGGAGG GCGCAGCTTT GGCGGATGCC TGTGAGGCTA TGGTGGCAGT GATGGCAGCC 
CTGGGTGTGG CAGTGGATGG TGGCAAGGAC TCCCTCAGCA TGGCTGCTCG GGTTGGCACT 
GAGACCGTGC GGGCTCCTGG GTCACTGGTC ATCTCAGCCT ATGCCGTCTG CCCAGACATC 
ACAGCCACTG TGACCCCAGA CCTCAAGCAT CCTGAAGGGA GAGGCCATCT GCTCTATGTG 
GCTCTGAGCC CTGGGCAGCA CCGGCTCGGG GGCACAGCTC TGGCCCAGTG CTTCTCCCAG 
CTTGGGGAAC ACCCTCCAGA CCTGGACCTT CCTGAGAACT TGGTGCGGGC CTTCAGCATC 
ACTCAGGGGC TGCTGAAAGA CCGCCTCCTC TGCTCAGGCC ACGATGTCAG TGACGGAGGC 
CTCGTCACAT GCCTGCTGGA GATGGCCTTT GCTGGAAATT GCGGGCTACA GGTGGATGTG 
CCTGTCCCCA GGGTTGATGT CCTGTCTGTG CTGTTCGCTG AGGAGCCAGG CCTCGTGCTG 
GAGGTGCAGG AGCCAGACCT GGCCCAGGTG CTGAAGCGTT ACCGGGATGC TGGCCTCCAT 
TGCCTGGAGC TGGGCCACAC AGGCGAGGCC GGGCCCCACG CCATGGTCCG GGTGTCAGTG 
AACGGGGCTG TGGTTCTGGA GGAGCCTGTT GGGGAGCTGC GAGCCCTCTG GGAGGAGACG 
AGTTTCCAGC TGGACCGGCT ACAGGCAGAG CCTCGCTGTG TGGCAGAGGA GGAACGGGGC 
CTGAGGGAGC GGATGGGGCC CAGCTATTGC CTGCCCCCCA CCTTTCCCAA AGCCTCCGTG 
CCCCGTGAGC CTGGTGGTCC CAGCCCCCGA GTCGCCATCT TGCGAGAGGA GGGCAGTAAT 
GGAGACCGGG AGATGGCCGA TGCCTTCCAC TTAGCTGGGT TTGAGGTATG GGACGTGACC 
ATGCAGGACC TCTGCTCTGG GGCAATTGGG CTGGACACTT TCCGTGGCGT GGCCTTCGTG 
GGCGGCTTCA GCTATGCAGA TGTCCTGGGC TCTGCCAAAG GGTGGGCAGC TGCTGTGACC 
TTTCATCCCA GGGCTGGGGC TGAGCTGAGG CGCTTCCGGA AGCGGCCAGA CACCTTCAGC 
CTGGGCGTGT GTAATGGCTG TCAACTGCTG GCTCTGCTCG GCTGGGTGGG AGGCGACCCC 
AATGAGGATG CTGCAGAGAT GGGCCCTGAC TCCCAGCCAG CCCGGCCAGG CCTTCTGCTA 
CGCCACAACC TGTCTGGGCG CTACGAGTCT CGCTGGGCCA GCGTGCGTGT GGGGCCTGGG 
CCAGCCCTGA TGCTGCGAGG GATGGAGGGC GCCGTGCTGC CCGTGTGGAG TGCGCACGGG 
GAAGGTTACG TAGCATTTTC TTCTCCGGAA CTCCAAGCTC AGATTGAGGC CAGGGGCTTG 
GCTCCACTGC ACTGGGCTGA TGATGACGGG AACCCCACAG AGCAGTACCC TCTGAATCCC 
AATGGGTCCC CAGGGGGCGT GGCTGGCATC TGCTCCTGTG ATGGCCGCCA CCTGGCTGTC 
ATGCCTCACC CTGAGCGGGC CGTTAGGCCT TGGCAGTGGG CATGGCGACC CCCTCCATTT 
GATACTCTGA CCACCTCCCC CTGGCTCCAG CTCTTTATCA ATGCCCGAAA CTGGACCCTG 
GAAGGGAGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880249.1
CDS	706..3960
Translation	MSTQESSNPNNVLKFCDNSSAIQGKEVRFLRPEDPTRPSRFQQQQGLRHVVFTAETHNFPTGVCPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPGNFARPLEVAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEADHISKEAPEPGMEVVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAPKGNPICSLHDQGAGGNGNVLKELSDPAGAIIYTSRFQLGDPTLNALEIWGAEYQESNALLLRSPNRDFLTHVSARERCPACFVGTITGDRRIVLVDDRECPVRRNGQGDAPPTPLPTPVDLELEWVLGKMPRKEFFLQRKPPMLQPLALPPGLSVHQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHEELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGTETVRAPGSLVISAYAVCPDITATVTPDLKHPEGRGHLLYVALSPGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFSITQGLLKDRLLCSGHDVSDGGLVTCLLEMAFAGNCGLQVDVPVPRVDVLSVLFAEEPGLVLEVQEPDLAQVLKRYRDAGLHCLELGHTGEAGPHAMVRVSVNGAVVLEEPVGELRALWEETSFQLDRLQAEPRCVAEEERGLRERMGPSYCLPPTFPKASVPREPGGPSPRVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPRAGAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGDPNEDAAEMGPDSQPARPGLLLRHNLSGRYESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYVAFSSPELQAQIEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSCDGRHLAVMPHPERAVRPWQWAWRPPPFDTLTTSPWLQLFINARNWTLEGSC

Target ORF information:

RefSeq Version	XM_017024760.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024760.1

ORF Insert Sequence:

ATGAGCACCC AGGAATCCTC GAACCCCAAC AACGTCCTCA AATTCTGTGA TAACAGCAGT 
GCAATCCAGG GAAAGGAAGT CCGATTCCTA CGGCCTGAGG ACCCCACACG GCCAAGCCGC 
TTCCAGCAAC AGCAAGGGCT GAGACATGTT GTCTTCACAG CAGAGACTCA CAACTTTCCC 
ACAGGAGTAT GCCCCTTTAG TGGTGCAACC ACTGGCACAG GGGGCCGGAT TCGAGATGTC 
CAGTGCACAG GCCGCGGGGC CCACGTGGTG GCTGGCACTG CCGGCTATTG CTTTGGAAAT 
CTGCATATTC CAGGTTACAA TCTGCCCTGG GAGGATCCAA GCTTCCAGTA TCCTGGGAAT 
TTTGCCCGGC CCCTGGAGGT TGCCATTGAA GCCAGTAATG GAGCTTCTGA CTATGGCAAC 
AAGTTTGGGG AACCAGTGCT GGCTGGCTTC GCCCGCTCCT TGGGCCTCCA GCTCCCAGAC 
GGCCAGCGGC GTGAGTGGAT CAAGCCCATC ATGTTTAGTG GGGGCATTGG GTCCATGGAA 
GCTGACCACA TAAGCAAGGA GGCCCCAGAG CCAGGCATGG AAGTTGTAAA GGTTGGAGGT 
CCCGTCTACA GGATTGGAGT TGGAGGTGGA GCTGCTTCAT CTGTGCAGGT GCAGGGAGAT 
AACACCAGTG ACCTGGACTT TGGGGCTGTG CAGCGAGGAG ACCCGGAGAT GGAACAGAAG 
ATGAACCGTG TGATCAGGGC TTGTGTGGAG GCCCCCAAGG GAAACCCCAT CTGCAGCCTT 
CATGATCAGG GCGCTGGTGG CAATGGCAAT GTCCTAAAAG AGCTGAGTGA CCCAGCTGGA 
GCCATCATTT ACACCAGCCG CTTCCAGCTT GGGGACCCAA CCCTGAATGC CCTGGAAATC 
TGGGGGGCTG AGTACCAGGA ATCAAATGCT CTTCTGCTGA GGTCCCCCAA CCGGGACTTC 
CTGACTCATG TCAGTGCCCG TGAACGTTGC CCGGCTTGCT TCGTGGGCAC CATCACTGGA 
GACCGGAGAA TAGTGCTGGT GGACGATCGG GAGTGTCCTG TCAGAAGAAA TGGCCAGGGG 
GATGCCCCCC CGACACCCCT GCCAACCCCT GTGGACCTGG AGCTCGAATG GGTGCTGGGC 
AAGATGCCTC GGAAGGAGTT CTTCCTGCAG AGGAAGCCCC CCATGCTGCA GCCTCTGGCC 
TTGCCCCCAG GGCTGAGCGT GCACCAGGCT CTGGAGAGGG TTCTGAGGCT GCCCGCCGTG 
GCCAGCAAGC GCTACCTCAC CAATAAGGTG GACCGCTCTG TGGGAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCCCT GCAAACTCCT CTGGCAGATG TAGCGGTTGT GGCACTGAGC 
CATGAGGAGC TCATAGGGGC TGCCACAGCC TTGGGAGAAC AGCCAGTCAA GAGCCTGCTG 
GACCCAAAAG TCGCCGCCCG GCTGGCCGTG GCCGAAGCCC TCACCAACCT GGTGTTTGCT 
CTGGTCACTG ACCTCCGGGA TGTGAAGTGT AGCGGGAACT GGATGTGGGC AGCCAAGCTC 
CCAGGGGAGG GCGCAGCTTT GGCGGATGCC TGTGAGGCTA TGGTGGCAGT GATGGCAGCC 
CTGGGTGTGG CAGTGGATGG TGGCAAGGAC TCCCTCAGCA TGGCTGCTCG GGTTGGCACT 
GAGACCGTGC GGGCTCCTGG GTCACTGGTC ATCTCAGCCT ATGCCGTCTG CCCAGACATC 
ACAGCCACTG TGACCCCAGA CCTCAAGCAT CCTGAAGGGA GAGGCCATCT GCTCTATGTG 
GCTCTGAGCC CTGGGCAGCA CCGGCTCGGG GGCACAGCTC TGGCCCAGTG CTTCTCCCAG 
CTTGGGGAAC ACCCTCCAGA CCTGGACCTT CCTGAGAACT TGGTGCGGGC CTTCAGCATC 
ACTCAGGGGC TGCTGAAAGA CCGCCTCCTC TGCTCAGGCC ACGATGTCAG TGACGGAGGC 
CTCGTCACAT GCCTGCTGGA GATGGCCTTT GCTGGAAATT GCGGGCTACA GGTGGATGTG 
CCTGTCCCCA GGGTTGATGT CCTGTCTGTG CTGTTCGCTG AGGAGCCAGG CCTCGTGCTG 
GAGGTGCAGG AGCCAGACCT GGCCCAGGTG CTGAAGCGTT ACCGGGATGC TGGCCTCCAT 
TGCCTGGAGC TGGGCCACAC AGGCGAGGCC GGGCCCCACG CCATGGTCCG GGTGTCAGTG 
AACGGGGCTG TGGTTCTGGA GGAGCCTGTT GGGGAGCTGC GAGCCCTCTG GGAGGAGACG 
AGTTTCCAGC TGGACCGGCT ACAGGCAGAG CCTCGCTGTG TGGCAGAGGA GGAACGGGGC 
CTGAGGGAGC GGATGGGGCC CAGCTATTGC CTGCCCCCCA CCTTTCCCAA AGCCTCCGTG 
CCCCGTGAGC CTGGTGGTCC CAGCCCCCGA GTCGCCATCT TGCGAGAGGA GGGCAGTAAT 
GGAGACCGGG AGATGGCCGA TGCCTTCCAC TTAGCTGGGT TTGAGGTATG GGACGTGACC 
ATGCAGGACC TCTGCTCTGG GGCAATTGGG CTGGACACTT TCCGTGGCGT GGCCTTCGTG 
GGCGGCTTCA GCTATGCAGA TGTCCTGGGC TCTGCCAAAG GGTGGGCAGC TGCTGTGACC 
TTTCATCCCA GGGCTGGGGC TGAGCTGAGG CGCTTCCGGA AGCGGCCAGA CACCTTCAGC 
CTGGGCGTGT GTAATGGCTG TCAACTGCTG GCTCTGCTCG GCTGGGTGGG AGGCGACCCC 
AATGAGGATG CTGCAGAGAT GGGCCCTGAC TCCCAGCCAG CCCGGCCAGG CCTTCTGCTA 
CGCCACAACC TGTCTGGGCG CTACGAGTCT CGCTGGGCCA GCGTGCGTGT GGGGCCTGGG 
CCAGCCCTGA TGCTGCGAGG GATGGAGGGC GCCGTGCTGC CCGTGTGGAG TGCGCACGGG 
GAAGGTTACG TAGCATTTTC TTCTCCGGAA CTCCAAGCTC AGATTGAGGC CAGGGGCTTG 
GCTCCACTGC ACTGGGCTGA TGATGACGGG AACCCCACAG AGCAGTACCC TCTGAATCCC 
AATGGGTCCC CAGGGGGCGT GGCTGGCATC TGCTCCTGTG ATGGCCGCCA CCTGGCTGTC 
ATGCCTCACC CTGAGCGGGC CGTTAGGCCT TGGCAGTGGG CATGGCGACC CCCTCCATTT 
GATACTCTGA CCACCTCCCC CTGGCTCCAG CTCTTTATCA ATGCCCGAAA CTGGACCCTG 
GAAGGGAGCT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( NM_012393.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036525.1 Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), mRNA.

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CloneID	OHu31922
Clone ID Related Accession (Same CDS sequence)	XM_024450804.1 , NM_012393.3 , NM_012393.2
Accession Version	NM_012393.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	phosphoribosylformylglycinamidine synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292804.1 and BC063538.1. On Nov 22, 2018 this sequence version replaced NM_012393.2. ##Evidence-Data-START## Transcript exon combination :: BC146768.1, SRR1660809.69783.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000314666.11/ ENSP00000313490.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCCCAG TCCTTCACTT CTATGTTCGT CCCTCTGGCC ATGAGGGGGC AGCCCCTGGA 
CACACTCGGA GGAAACTGCA AGGGAAACTG CCAGAGCTGC AGGGCGTCGA GACTGAACTG 
TGCTACAACG TGAACTGGAC AGCTGAGGCC CTCCCCAGTG CTGAGGAGAC AAAGAAGCTG 
ATGTGGCTGT TTGGTTGCCC CTTACTGCTG GATGATGTTG CTCGGGAGTC CTGGCTCCTT 
CCTGGCTCCA ATGACCTGCT GCTGGAGGTC GGGCCCAGGC TGAACTTCTC CACCCCAACA 
TCCACCAACA TCGTGTCAGT GTGCCGCGCC ACTGGGCTGG GGCCTGTGGA TCGTGTGGAG 
ACCACCCGGC GCTACCGGCT CTCGTTTGCC CACCCCCCGT CAGCTGAGGT GGAAGCCATT 
GCTCTGGCTA CCCTGCACGA CCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGT 
TTCTCCCCTG AGAGCATGCC GGAACCCCTC AATGGCCCTA TCAATATACT GGGTGAGGGC 
CGGCTTGCGC TGGAGAAGGC CAACCAGGAG CTTGGTCTGG CTTTAGACTC TTGGGACCTA 
GACTTCTACA CCAAGCGCTT CCAGGAGCTA CAGCGGAACC CGAGCACTGT GGAGGCCTTT 
GACTTGGCGC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
GTGGATGGGC AGAAGCTGGT GCACTCACTG TTTGAGTCCA TCATGAGCAC CCAGGAATCC 
TCGAACCCCA ACAACGTCCT CAAATTCTGT GATAACAGCA GTGCAATCCA GGGAAAGGAA 
GTCCGATTCC TACGGCCTGA GGACCCCACA CGGCCAAGCC GCTTCCAGCA ACAGCAAGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACT CACAACTTTC CCACAGGAGT ATGCCCCTTT 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATTCGAGATG TCCAGTGCAC AGGCCGCGGG 
GCCCACGTGG TGGCTGGCAC TGCCGGCTAT TGCTTTGGAA ATCTGCATAT TCCAGGTTAC 
AATCTGCCCT GGGAGGATCC AAGCTTCCAG TATCCTGGGA ATTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AAGCCAGTAA TGGAGCTTCT GACTATGGCA ACAAGTTTGG GGAACCAGTG 
CTGGCTGGCT TCGCCCGCTC CTTGGGCCTC CAGCTCCCAG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGGGGCATT GGGTCCATGG AAGCTGACCA CATAAGCAAG 
GAGGCCCCAG AGCCAGGCAT GGAAGTTGTA AAGGTTGGAG GTCCCGTCTA CAGGATTGGA 
GTTGGAGGTG GAGCTGCTTC ATCTGTGCAG GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGAGG AGACCCGGAG ATGGAACAGA AGATGAACCG TGTGATCAGG 
GCTTGTGTGG AGGCCCCCAA GGGAAACCCC ATCTGCAGCC TTCATGATCA GGGCGCTGGT 
GGCAATGGCA ATGTCCTAAA AGAGCTGAGT GACCCAGCTG GAGCCATCAT TTACACCAGC 
CGCTTCCAGC TTGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC TGAGTACCAG 
GAATCAAATG CTCTTCTGCT GAGGTCCCCC AACCGGGACT TCCTGACTCA TGTCAGTGCC 
CGTGAACGTT GCCCGGCTTG CTTCGTGGGC ACCATCACTG GAGACCGGAG AATAGTGCTG 
GTGGACGATC GGGAGTGTCC TGTCAGAAGA AATGGCCAGG GGGATGCCCC CCCGACACCC 
CTGCCAACCC CTGTGGACCT GGAGCTCGAA TGGGTGCTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTGC AGAGGAAGCC CCCCATGCTG CAGCCTCTGG CCTTGCCCCC AGGGCTGAGC 
GTGCACCAGG CTCTGGAGAG GGTTCTGAGG CTGCCCGCCG TGGCCAGCAA GCGCTACCTC 
ACCAATAAGG TGGACCGCTC TGTGGGAGGC CTGGTGGCCC AGCAGCAGTG CGTGGGGCCC 
CTGCAAACTC CTCTGGCAGA TGTAGCGGTT GTGGCACTGA GCCATGAGGA GCTCATAGGG 
GCTGCCACAG CCTTGGGAGA ACAGCCAGTC AAGAGCCTGC TGGACCCAAA AGTCGCCGCC 
CGGCTGGCCG TGGCCGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC TGACCTCCGG 
GATGTGAAGT GTAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGCGCAGCT 
TTGGCGGATG CCTGTGAGGC TATGGTGGCA GTGATGGCAG CCCTGGGTGT GGCAGTGGAT 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCT CGGGTTGGCA CTGAGACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCCGTC TGCCCAGACA TCACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGAAGG GAGAGGCCAT CTGCTCTATG TGGCTCTGAG CCCTGGGCAG 
CACCGGCTCG GGGGCACAGC TCTGGCCCAG TGCTTCTCCC AGCTTGGGGA ACACCCTCCA 
GACCTGGACC TTCCTGAGAA CTTGGTGCGG GCCTTCAGCA TCACTCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCACGATGTC AGTGACGGAG GCCTCGTCAC ATGCCTGCTG 
GAGATGGCCT TTGCTGGAAA TTGCGGGCTA CAGGTGGATG TGCCTGTCCC CAGGGTTGAT 
GTCCTGTCTG TGCTGTTCGC TGAGGAGCCA GGCCTCGTGC TGGAGGTGCA GGAGCCAGAC 
CTGGCCCAGG TGCTGAAGCG TTACCGGGAT GCTGGCCTCC ATTGCCTGGA GCTGGGCCAC 
ACAGGCGAGG CCGGGCCCCA CGCCATGGTC CGGGTGTCAG TGAACGGGGC TGTGGTTCTG 
GAGGAGCCTG TTGGGGAGCT GCGAGCCCTC TGGGAGGAGA CGAGTTTCCA GCTGGACCGG 
CTACAGGCAG AGCCTCGCTG TGTGGCAGAG GAGGAACGGG GCCTGAGGGA GCGGATGGGG 
CCCAGCTATT GCCTGCCCCC CACCTTTCCC AAAGCCTCCG TGCCCCGTGA GCCTGGTGGT 
CCCAGCCCCC GAGTCGCCAT CTTGCGAGAG GAGGGCAGTA ATGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTAGCTGG GTTTGAGGTA TGGGACGTGA CCATGCAGGA CCTCTGCTCT 
GGGGCAATTG GGCTGGACAC TTTCCGTGGC GTGGCCTTCG TGGGCGGCTT CAGCTATGCA 
GATGTCCTGG GCTCTGCCAA AGGGTGGGCA GCTGCTGTGA CCTTTCATCC CAGGGCTGGG 
GCTGAGCTGA GGCGCTTCCG GAAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAATGGC 
TGTCAACTGC TGGCTCTGCT CGGCTGGGTG GGAGGCGACC CCAATGAGGA TGCTGCAGAG 
ATGGGCCCTG ACTCCCAGCC AGCCCGGCCA GGCCTTCTGC TACGCCACAA CCTGTCTGGG 
CGCTACGAGT CTCGCTGGGC CAGCGTGCGT GTGGGGCCTG GGCCAGCCCT GATGCTGCGA 
GGGATGGAGG GCGCCGTGCT GCCCGTGTGG AGTGCGCACG GGGAAGGTTA CGTAGCATTT 
TCTTCTCCGG AACTCCAAGC TCAGATTGAG GCCAGGGGCT TGGCTCCACT GCACTGGGCT 
GATGATGACG GGAACCCCAC AGAGCAGTAC CCTCTGAATC CCAATGGGTC CCCAGGGGGC 
GTGGCTGGCA TCTGCTCCTG TGATGGCCGC CACCTGGCTG TCATGCCTCA CCCTGAGCGG 
GCCGTTAGGC CTTGGCAGTG GGCATGGCGA CCCCCTCCAT TTGATACTCT GACCACCTCC 
CCCTGGCTCC AGCTCTTTAT CAATGCCCGA AACTGGACCC TGGAAGGGAG CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036525.1
CDS	131..4147
Translation	MSPVLHFYVRPSGHEGAAPGHTRRKLQGKLPELQGVETELCYNVNWTAEALPSAEETKKLMWLFGCPLLLDDVARESWLLPGSNDLLLEVGPRLNFSTPTSTNIVSVCRATGLGPVDRVETTRRYRLSFAHPPSAEVEAIALATLHDRMTEQHFPHPIQSFSPESMPEPLNGPINILGEGRLALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHVDGQKLVHSLFESIMSTQESSNPNNVLKFCDNSSAIQGKEVRFLRPEDPTRPSRFQQQQGLRHVVFTAETHNFPTGVCPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPGNFARPLEVAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEADHISKEAPEPGMEVVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAPKGNPICSLHDQGAGGNGNVLKELSDPAGAIIYTSRFQLGDPTLNALEIWGAEYQESNALLLRSPNRDFLTHVSARERCPACFVGTITGDRRIVLVDDRECPVRRNGQGDAPPTPLPTPVDLELEWVLGKMPRKEFFLQRKPPMLQPLALPPGLSVHQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHEELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGTETVRAPGSLVISAYAVCPDITATVTPDLKHPEGRGHLLYVALSPGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFSITQGLLKDRLLCSGHDVSDGGLVTCLLEMAFAGNCGLQVDVPVPRVDVLSVLFAEEPGLVLEVQEPDLAQVLKRYRDAGLHCLELGHTGEAGPHAMVRVSVNGAVVLEEPVGELRALWEETSFQLDRLQAEPRCVAEEERGLRERMGPSYCLPPTFPKASVPREPGGPSPRVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPRAGAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGDPNEDAAEMGPDSQPARPGLLLRHNLSGRYESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYVAFSSPELQAQIEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSCDGRHLAVMPHPERAVRPWQWAWRPPPFDTLTTSPWLQLFINARNWTLEGSC

Target ORF information:

RefSeq Version	NM_012393.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012393.3

ORF Insert Sequence:

ATGTCCCCAG TCCTTCACTT CTATGTTCGT CCCTCTGGCC ATGAGGGGGC AGCCCCTGGA 
CACACTCGGA GGAAACTGCA AGGGAAACTG CCAGAGCTGC AGGGCGTCGA GACTGAACTG 
TGCTACAACG TGAACTGGAC AGCTGAGGCC CTCCCCAGTG CTGAGGAGAC AAAGAAGCTG 
ATGTGGCTGT TTGGTTGCCC CTTACTGCTG GATGATGTTG CTCGGGAGTC CTGGCTCCTT 
CCTGGCTCCA ATGACCTGCT GCTGGAGGTC GGGCCCAGGC TGAACTTCTC CACCCCAACA 
TCCACCAACA TCGTGTCAGT GTGCCGCGCC ACTGGGCTGG GGCCTGTGGA TCGTGTGGAG 
ACCACCCGGC GCTACCGGCT CTCGTTTGCC CACCCCCCGT CAGCTGAGGT GGAAGCCATT 
GCTCTGGCTA CCCTGCACGA CCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGT 
TTCTCCCCTG AGAGCATGCC GGAACCCCTC AATGGCCCTA TCAATATACT GGGTGAGGGC 
CGGCTTGCGC TGGAGAAGGC CAACCAGGAG CTTGGTCTGG CTTTAGACTC TTGGGACCTA 
GACTTCTACA CCAAGCGCTT CCAGGAGCTA CAGCGGAACC CGAGCACTGT GGAGGCCTTT 
GACTTGGCGC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
GTGGATGGGC AGAAGCTGGT GCACTCACTG TTTGAGTCCA TCATGAGCAC CCAGGAATCC 
TCGAACCCCA ACAACGTCCT CAAATTCTGT GATAACAGCA GTGCAATCCA GGGAAAGGAA 
GTCCGATTCC TACGGCCTGA GGACCCCACA CGGCCAAGCC GCTTCCAGCA ACAGCAAGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACT CACAACTTTC CCACAGGAGT ATGCCCCTTT 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATTCGAGATG TCCAGTGCAC AGGCCGCGGG 
GCCCACGTGG TGGCTGGCAC TGCCGGCTAT TGCTTTGGAA ATCTGCATAT TCCAGGTTAC 
AATCTGCCCT GGGAGGATCC AAGCTTCCAG TATCCTGGGA ATTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AAGCCAGTAA TGGAGCTTCT GACTATGGCA ACAAGTTTGG GGAACCAGTG 
CTGGCTGGCT TCGCCCGCTC CTTGGGCCTC CAGCTCCCAG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGGGGCATT GGGTCCATGG AAGCTGACCA CATAAGCAAG 
GAGGCCCCAG AGCCAGGCAT GGAAGTTGTA AAGGTTGGAG GTCCCGTCTA CAGGATTGGA 
GTTGGAGGTG GAGCTGCTTC ATCTGTGCAG GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGAGG AGACCCGGAG ATGGAACAGA AGATGAACCG TGTGATCAGG 
GCTTGTGTGG AGGCCCCCAA GGGAAACCCC ATCTGCAGCC TTCATGATCA GGGCGCTGGT 
GGCAATGGCA ATGTCCTAAA AGAGCTGAGT GACCCAGCTG GAGCCATCAT TTACACCAGC 
CGCTTCCAGC TTGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC TGAGTACCAG 
GAATCAAATG CTCTTCTGCT GAGGTCCCCC AACCGGGACT TCCTGACTCA TGTCAGTGCC 
CGTGAACGTT GCCCGGCTTG CTTCGTGGGC ACCATCACTG GAGACCGGAG AATAGTGCTG 
GTGGACGATC GGGAGTGTCC TGTCAGAAGA AATGGCCAGG GGGATGCCCC CCCGACACCC 
CTGCCAACCC CTGTGGACCT GGAGCTCGAA TGGGTGCTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTGC AGAGGAAGCC CCCCATGCTG CAGCCTCTGG CCTTGCCCCC AGGGCTGAGC 
GTGCACCAGG CTCTGGAGAG GGTTCTGAGG CTGCCCGCCG TGGCCAGCAA GCGCTACCTC 
ACCAATAAGG TGGACCGCTC TGTGGGAGGC CTGGTGGCCC AGCAGCAGTG CGTGGGGCCC 
CTGCAAACTC CTCTGGCAGA TGTAGCGGTT GTGGCACTGA GCCATGAGGA GCTCATAGGG 
GCTGCCACAG CCTTGGGAGA ACAGCCAGTC AAGAGCCTGC TGGACCCAAA AGTCGCCGCC 
CGGCTGGCCG TGGCCGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC TGACCTCCGG 
GATGTGAAGT GTAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGCGCAGCT 
TTGGCGGATG CCTGTGAGGC TATGGTGGCA GTGATGGCAG CCCTGGGTGT GGCAGTGGAT 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCT CGGGTTGGCA CTGAGACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCCGTC TGCCCAGACA TCACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGAAGG GAGAGGCCAT CTGCTCTATG TGGCTCTGAG CCCTGGGCAG 
CACCGGCTCG GGGGCACAGC TCTGGCCCAG TGCTTCTCCC AGCTTGGGGA ACACCCTCCA 
GACCTGGACC TTCCTGAGAA CTTGGTGCGG GCCTTCAGCA TCACTCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCACGATGTC AGTGACGGAG GCCTCGTCAC ATGCCTGCTG 
GAGATGGCCT TTGCTGGAAA TTGCGGGCTA CAGGTGGATG TGCCTGTCCC CAGGGTTGAT 
GTCCTGTCTG TGCTGTTCGC TGAGGAGCCA GGCCTCGTGC TGGAGGTGCA GGAGCCAGAC 
CTGGCCCAGG TGCTGAAGCG TTACCGGGAT GCTGGCCTCC ATTGCCTGGA GCTGGGCCAC 
ACAGGCGAGG CCGGGCCCCA CGCCATGGTC CGGGTGTCAG TGAACGGGGC TGTGGTTCTG 
GAGGAGCCTG TTGGGGAGCT GCGAGCCCTC TGGGAGGAGA CGAGTTTCCA GCTGGACCGG 
CTACAGGCAG AGCCTCGCTG TGTGGCAGAG GAGGAACGGG GCCTGAGGGA GCGGATGGGG 
CCCAGCTATT GCCTGCCCCC CACCTTTCCC AAAGCCTCCG TGCCCCGTGA GCCTGGTGGT 
CCCAGCCCCC GAGTCGCCAT CTTGCGAGAG GAGGGCAGTA ATGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTAGCTGG GTTTGAGGTA TGGGACGTGA CCATGCAGGA CCTCTGCTCT 
GGGGCAATTG GGCTGGACAC TTTCCGTGGC GTGGCCTTCG TGGGCGGCTT CAGCTATGCA 
GATGTCCTGG GCTCTGCCAA AGGGTGGGCA GCTGCTGTGA CCTTTCATCC CAGGGCTGGG 
GCTGAGCTGA GGCGCTTCCG GAAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAATGGC 
TGTCAACTGC TGGCTCTGCT CGGCTGGGTG GGAGGCGACC CCAATGAGGA TGCTGCAGAG 
ATGGGCCCTG ACTCCCAGCC AGCCCGGCCA GGCCTTCTGC TACGCCACAA CCTGTCTGGG 
CGCTACGAGT CTCGCTGGGC CAGCGTGCGT GTGGGGCCTG GGCCAGCCCT GATGCTGCGA 
GGGATGGAGG GCGCCGTGCT GCCCGTGTGG AGTGCGCACG GGGAAGGTTA CGTAGCATTT 
TCTTCTCCGG AACTCCAAGC TCAGATTGAG GCCAGGGGCT TGGCTCCACT GCACTGGGCT 
GATGATGACG GGAACCCCAC AGAGCAGTAC CCTCTGAATC CCAATGGGTC CCCAGGGGGC 
GTGGCTGGCA TCTGCTCCTG TGATGGCCGC CACCTGGCTG TCATGCCTCA CCCTGAGCGG 
GCCGTTAGGC CTTGGCAGTG GGCATGGCGA CCCCCTCCAT TTGATACTCT GACCACCTCC 
CCCTGGCTCC AGCTCTTTAT CAATGCCCGA AACTGGACCC TGGAAGGGAG CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( NM_012393.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_036525.1 Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), mRNA.

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CloneID	OHu31922
Clone ID Related Accession (Same CDS sequence)	XM_024450804.1 , NM_012393.3 , NM_012393.2
Accession Version	NM_012393.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	phosphoribosylformylglycinamidine synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292804.1, BC063538.1 and AL044326.1. On Aug 8, 2008 this sequence version replaced NM_012393.1. ##Evidence-Data-START## Transcript exon combination :: AK292804.1, SRR1660809.69783.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCCCAG TCCTTCACTT CTATGTTCGT CCCTCTGGCC ATGAGGGGGC AGCCCCTGGA 
CACACTCGGA GGAAACTGCA AGGGAAACTG CCAGAGCTGC AGGGCGTCGA GACTGAACTG 
TGCTACAACG TGAACTGGAC AGCTGAGGCC CTCCCCAGTG CTGAGGAGAC AAAGAAGCTG 
ATGTGGCTGT TTGGTTGCCC CTTACTGCTG GATGATGTTG CTCGGGAGTC CTGGCTCCTT 
CCTGGCTCCA ATGACCTGCT GCTGGAGGTC GGGCCCAGGC TGAACTTCTC CACCCCAACA 
TCCACCAACA TCGTGTCAGT GTGCCGCGCC ACTGGGCTGG GGCCTGTGGA TCGTGTGGAG 
ACCACCCGGC GCTACCGGCT CTCGTTTGCC CACCCCCCGT CAGCTGAGGT GGAAGCCATT 
GCTCTGGCTA CCCTGCACGA CCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGT 
TTCTCCCCTG AGAGCATGCC GGAACCCCTC AATGGCCCTA TCAATATACT GGGTGAGGGC 
CGGCTTGCGC TGGAGAAGGC CAACCAGGAG CTTGGTCTGG CTTTAGACTC TTGGGACCTA 
GACTTCTACA CCAAGCGCTT CCAGGAGCTA CAGCGGAACC CGAGCACTGT GGAGGCCTTT 
GACTTGGCGC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
GTGGATGGGC AGAAGCTGGT GCACTCACTG TTTGAGTCCA TCATGAGCAC CCAGGAATCC 
TCGAACCCCA ACAACGTCCT CAAATTCTGT GATAACAGCA GTGCAATCCA GGGAAAGGAA 
GTCCGATTCC TACGGCCTGA GGACCCCACA CGGCCAAGCC GCTTCCAGCA ACAGCAAGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACT CACAACTTTC CCACAGGAGT ATGCCCCTTT 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATTCGAGATG TCCAGTGCAC AGGCCGCGGG 
GCCCACGTGG TGGCTGGCAC TGCCGGCTAT TGCTTTGGAA ATCTGCATAT TCCAGGTTAC 
AATCTGCCCT GGGAGGATCC AAGCTTCCAG TATCCTGGGA ATTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AAGCCAGTAA TGGAGCTTCT GACTATGGCA ACAAGTTTGG GGAACCAGTG 
CTGGCTGGCT TCGCCCGCTC CTTGGGCCTC CAGCTCCCAG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGGGGCATT GGGTCCATGG AAGCTGACCA CATAAGCAAG 
GAGGCCCCAG AGCCAGGCAT GGAAGTTGTA AAGGTTGGAG GTCCCGTCTA CAGGATTGGA 
GTTGGAGGTG GAGCTGCTTC ATCTGTGCAG GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGAGG AGACCCGGAG ATGGAACAGA AGATGAACCG TGTGATCAGG 
GCTTGTGTGG AGGCCCCCAA GGGAAACCCC ATCTGCAGCC TTCATGATCA GGGCGCTGGT 
GGCAATGGCA ATGTCCTAAA AGAGCTGAGT GACCCAGCTG GAGCCATCAT TTACACCAGC 
CGCTTCCAGC TTGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC TGAGTACCAG 
GAATCAAATG CTCTTCTGCT GAGGTCCCCC AACCGGGACT TCCTGACTCA TGTCAGTGCC 
CGTGAACGTT GCCCGGCTTG CTTCGTGGGC ACCATCACTG GAGACCGGAG AATAGTGCTG 
GTGGACGATC GGGAGTGTCC TGTCAGAAGA AATGGCCAGG GGGATGCCCC CCCGACACCC 
CTGCCAACCC CTGTGGACCT GGAGCTCGAA TGGGTGCTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTGC AGAGGAAGCC CCCCATGCTG CAGCCTCTGG CCTTGCCCCC AGGGCTGAGC 
GTGCACCAGG CTCTGGAGAG GGTTCTGAGG CTGCCCGCCG TGGCCAGCAA GCGCTACCTC 
ACCAATAAGG TGGACCGCTC TGTGGGAGGC CTGGTGGCCC AGCAGCAGTG CGTGGGGCCC 
CTGCAAACTC CTCTGGCAGA TGTAGCGGTT GTGGCACTGA GCCATGAGGA GCTCATAGGG 
GCTGCCACAG CCTTGGGAGA ACAGCCAGTC AAGAGCCTGC TGGACCCAAA AGTCGCCGCC 
CGGCTGGCCG TGGCCGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC TGACCTCCGG 
GATGTGAAGT GTAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGCGCAGCT 
TTGGCGGATG CCTGTGAGGC TATGGTGGCA GTGATGGCAG CCCTGGGTGT GGCAGTGGAT 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCT CGGGTTGGCA CTGAGACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCCGTC TGCCCAGACA TCACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGAAGG GAGAGGCCAT CTGCTCTATG TGGCTCTGAG CCCTGGGCAG 
CACCGGCTCG GGGGCACAGC TCTGGCCCAG TGCTTCTCCC AGCTTGGGGA ACACCCTCCA 
GACCTGGACC TTCCTGAGAA CTTGGTGCGG GCCTTCAGCA TCACTCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCACGATGTC AGTGACGGAG GCCTCGTCAC ATGCCTGCTG 
GAGATGGCCT TTGCTGGAAA TTGCGGGCTA CAGGTGGATG TGCCTGTCCC CAGGGTTGAT 
GTCCTGTCTG TGCTGTTCGC TGAGGAGCCA GGCCTCGTGC TGGAGGTGCA GGAGCCAGAC 
CTGGCCCAGG TGCTGAAGCG TTACCGGGAT GCTGGCCTCC ATTGCCTGGA GCTGGGCCAC 
ACAGGCGAGG CCGGGCCCCA CGCCATGGTC CGGGTGTCAG TGAACGGGGC TGTGGTTCTG 
GAGGAGCCTG TTGGGGAGCT GCGAGCCCTC TGGGAGGAGA CGAGTTTCCA GCTGGACCGG 
CTACAGGCAG AGCCTCGCTG TGTGGCAGAG GAGGAACGGG GCCTGAGGGA GCGGATGGGG 
CCCAGCTATT GCCTGCCCCC CACCTTTCCC AAAGCCTCCG TGCCCCGTGA GCCTGGTGGT 
CCCAGCCCCC GAGTCGCCAT CTTGCGAGAG GAGGGCAGTA ATGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTAGCTGG GTTTGAGGTA TGGGACGTGA CCATGCAGGA CCTCTGCTCT 
GGGGCAATTG GGCTGGACAC TTTCCGTGGC GTGGCCTTCG TGGGCGGCTT CAGCTATGCA 
GATGTCCTGG GCTCTGCCAA AGGGTGGGCA GCTGCTGTGA CCTTTCATCC CAGGGCTGGG 
GCTGAGCTGA GGCGCTTCCG GAAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAATGGC 
TGTCAACTGC TGGCTCTGCT CGGCTGGGTG GGAGGCGACC CCAATGAGGA TGCTGCAGAG 
ATGGGCCCTG ACTCCCAGCC AGCCCGGCCA GGCCTTCTGC TACGCCACAA CCTGTCTGGG 
CGCTACGAGT CTCGCTGGGC CAGCGTGCGT GTGGGGCCTG GGCCAGCCCT GATGCTGCGA 
GGGATGGAGG GCGCCGTGCT GCCCGTGTGG AGTGCGCACG GGGAAGGTTA CGTAGCATTT 
TCTTCTCCGG AACTCCAAGC TCAGATTGAG GCCAGGGGCT TGGCTCCACT GCACTGGGCT 
GATGATGACG GGAACCCCAC AGAGCAGTAC CCTCTGAATC CCAATGGGTC CCCAGGGGGC 
GTGGCTGGCA TCTGCTCCTG TGATGGCCGC CACCTGGCTG TCATGCCTCA CCCTGAGCGG 
GCCGTTAGGC CTTGGCAGTG GGCATGGCGA CCCCCTCCAT TTGATACTCT GACCACCTCC 
CCCTGGCTCC AGCTCTTTAT CAATGCCCGA AACTGGACCC TGGAAGGGAG CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036525.1
CDS	142..4158
Translation	MSPVLHFYVRPSGHEGAAPGHTRRKLQGKLPELQGVETELCYNVNWTAEALPSAEETKKLMWLFGCPLLLDDVARESWLLPGSNDLLLEVGPRLNFSTPTSTNIVSVCRATGLGPVDRVETTRRYRLSFAHPPSAEVEAIALATLHDRMTEQHFPHPIQSFSPESMPEPLNGPINILGEGRLALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHVDGQKLVHSLFESIMSTQESSNPNNVLKFCDNSSAIQGKEVRFLRPEDPTRPSRFQQQQGLRHVVFTAETHNFPTGVCPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPGNFARPLEVAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEADHISKEAPEPGMEVVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAPKGNPICSLHDQGAGGNGNVLKELSDPAGAIIYTSRFQLGDPTLNALEIWGAEYQESNALLLRSPNRDFLTHVSARERCPACFVGTITGDRRIVLVDDRECPVRRNGQGDAPPTPLPTPVDLELEWVLGKMPRKEFFLQRKPPMLQPLALPPGLSVHQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHEELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGTETVRAPGSLVISAYAVCPDITATVTPDLKHPEGRGHLLYVALSPGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFSITQGLLKDRLLCSGHDVSDGGLVTCLLEMAFAGNCGLQVDVPVPRVDVLSVLFAEEPGLVLEVQEPDLAQVLKRYRDAGLHCLELGHTGEAGPHAMVRVSVNGAVVLEEPVGELRALWEETSFQLDRLQAEPRCVAEEERGLRERMGPSYCLPPTFPKASVPREPGGPSPRVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPRAGAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGDPNEDAAEMGPDSQPARPGLLLRHNLSGRYESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYVAFSSPELQAQIEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSCDGRHLAVMPHPERAVRPWQWAWRPPPFDTLTTSPWLQLFINARNWTLEGSC

Target ORF information:

RefSeq Version	NM_012393.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012393.2

ORF Insert Sequence:

ATGTCCCCAG TCCTTCACTT CTATGTTCGT CCCTCTGGCC ATGAGGGGGC AGCCCCTGGA 
CACACTCGGA GGAAACTGCA AGGGAAACTG CCAGAGCTGC AGGGCGTCGA GACTGAACTG 
TGCTACAACG TGAACTGGAC AGCTGAGGCC CTCCCCAGTG CTGAGGAGAC AAAGAAGCTG 
ATGTGGCTGT TTGGTTGCCC CTTACTGCTG GATGATGTTG CTCGGGAGTC CTGGCTCCTT 
CCTGGCTCCA ATGACCTGCT GCTGGAGGTC GGGCCCAGGC TGAACTTCTC CACCCCAACA 
TCCACCAACA TCGTGTCAGT GTGCCGCGCC ACTGGGCTGG GGCCTGTGGA TCGTGTGGAG 
ACCACCCGGC GCTACCGGCT CTCGTTTGCC CACCCCCCGT CAGCTGAGGT GGAAGCCATT 
GCTCTGGCTA CCCTGCACGA CCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGT 
TTCTCCCCTG AGAGCATGCC GGAACCCCTC AATGGCCCTA TCAATATACT GGGTGAGGGC 
CGGCTTGCGC TGGAGAAGGC CAACCAGGAG CTTGGTCTGG CTTTAGACTC TTGGGACCTA 
GACTTCTACA CCAAGCGCTT CCAGGAGCTA CAGCGGAACC CGAGCACTGT GGAGGCCTTT 
GACTTGGCGC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
GTGGATGGGC AGAAGCTGGT GCACTCACTG TTTGAGTCCA TCATGAGCAC CCAGGAATCC 
TCGAACCCCA ACAACGTCCT CAAATTCTGT GATAACAGCA GTGCAATCCA GGGAAAGGAA 
GTCCGATTCC TACGGCCTGA GGACCCCACA CGGCCAAGCC GCTTCCAGCA ACAGCAAGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACT CACAACTTTC CCACAGGAGT ATGCCCCTTT 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATTCGAGATG TCCAGTGCAC AGGCCGCGGG 
GCCCACGTGG TGGCTGGCAC TGCCGGCTAT TGCTTTGGAA ATCTGCATAT TCCAGGTTAC 
AATCTGCCCT GGGAGGATCC AAGCTTCCAG TATCCTGGGA ATTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AAGCCAGTAA TGGAGCTTCT GACTATGGCA ACAAGTTTGG GGAACCAGTG 
CTGGCTGGCT TCGCCCGCTC CTTGGGCCTC CAGCTCCCAG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGGGGCATT GGGTCCATGG AAGCTGACCA CATAAGCAAG 
GAGGCCCCAG AGCCAGGCAT GGAAGTTGTA AAGGTTGGAG GTCCCGTCTA CAGGATTGGA 
GTTGGAGGTG GAGCTGCTTC ATCTGTGCAG GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGAGG AGACCCGGAG ATGGAACAGA AGATGAACCG TGTGATCAGG 
GCTTGTGTGG AGGCCCCCAA GGGAAACCCC ATCTGCAGCC TTCATGATCA GGGCGCTGGT 
GGCAATGGCA ATGTCCTAAA AGAGCTGAGT GACCCAGCTG GAGCCATCAT TTACACCAGC 
CGCTTCCAGC TTGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC TGAGTACCAG 
GAATCAAATG CTCTTCTGCT GAGGTCCCCC AACCGGGACT TCCTGACTCA TGTCAGTGCC 
CGTGAACGTT GCCCGGCTTG CTTCGTGGGC ACCATCACTG GAGACCGGAG AATAGTGCTG 
GTGGACGATC GGGAGTGTCC TGTCAGAAGA AATGGCCAGG GGGATGCCCC CCCGACACCC 
CTGCCAACCC CTGTGGACCT GGAGCTCGAA TGGGTGCTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTGC AGAGGAAGCC CCCCATGCTG CAGCCTCTGG CCTTGCCCCC AGGGCTGAGC 
GTGCACCAGG CTCTGGAGAG GGTTCTGAGG CTGCCCGCCG TGGCCAGCAA GCGCTACCTC 
ACCAATAAGG TGGACCGCTC TGTGGGAGGC CTGGTGGCCC AGCAGCAGTG CGTGGGGCCC 
CTGCAAACTC CTCTGGCAGA TGTAGCGGTT GTGGCACTGA GCCATGAGGA GCTCATAGGG 
GCTGCCACAG CCTTGGGAGA ACAGCCAGTC AAGAGCCTGC TGGACCCAAA AGTCGCCGCC 
CGGCTGGCCG TGGCCGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC TGACCTCCGG 
GATGTGAAGT GTAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGCGCAGCT 
TTGGCGGATG CCTGTGAGGC TATGGTGGCA GTGATGGCAG CCCTGGGTGT GGCAGTGGAT 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCT CGGGTTGGCA CTGAGACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCCGTC TGCCCAGACA TCACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGAAGG GAGAGGCCAT CTGCTCTATG TGGCTCTGAG CCCTGGGCAG 
CACCGGCTCG GGGGCACAGC TCTGGCCCAG TGCTTCTCCC AGCTTGGGGA ACACCCTCCA 
GACCTGGACC TTCCTGAGAA CTTGGTGCGG GCCTTCAGCA TCACTCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCACGATGTC AGTGACGGAG GCCTCGTCAC ATGCCTGCTG 
GAGATGGCCT TTGCTGGAAA TTGCGGGCTA CAGGTGGATG TGCCTGTCCC CAGGGTTGAT 
GTCCTGTCTG TGCTGTTCGC TGAGGAGCCA GGCCTCGTGC TGGAGGTGCA GGAGCCAGAC 
CTGGCCCAGG TGCTGAAGCG TTACCGGGAT GCTGGCCTCC ATTGCCTGGA GCTGGGCCAC 
ACAGGCGAGG CCGGGCCCCA CGCCATGGTC CGGGTGTCAG TGAACGGGGC TGTGGTTCTG 
GAGGAGCCTG TTGGGGAGCT GCGAGCCCTC TGGGAGGAGA CGAGTTTCCA GCTGGACCGG 
CTACAGGCAG AGCCTCGCTG TGTGGCAGAG GAGGAACGGG GCCTGAGGGA GCGGATGGGG 
CCCAGCTATT GCCTGCCCCC CACCTTTCCC AAAGCCTCCG TGCCCCGTGA GCCTGGTGGT 
CCCAGCCCCC GAGTCGCCAT CTTGCGAGAG GAGGGCAGTA ATGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTAGCTGG GTTTGAGGTA TGGGACGTGA CCATGCAGGA CCTCTGCTCT 
GGGGCAATTG GGCTGGACAC TTTCCGTGGC GTGGCCTTCG TGGGCGGCTT CAGCTATGCA 
GATGTCCTGG GCTCTGCCAA AGGGTGGGCA GCTGCTGTGA CCTTTCATCC CAGGGCTGGG 
GCTGAGCTGA GGCGCTTCCG GAAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAATGGC 
TGTCAACTGC TGGCTCTGCT CGGCTGGGTG GGAGGCGACC CCAATGAGGA TGCTGCAGAG 
ATGGGCCCTG ACTCCCAGCC AGCCCGGCCA GGCCTTCTGC TACGCCACAA CCTGTCTGGG 
CGCTACGAGT CTCGCTGGGC CAGCGTGCGT GTGGGGCCTG GGCCAGCCCT GATGCTGCGA 
GGGATGGAGG GCGCCGTGCT GCCCGTGTGG AGTGCGCACG GGGAAGGTTA CGTAGCATTT 
TCTTCTCCGG AACTCCAAGC TCAGATTGAG GCCAGGGGCT TGGCTCCACT GCACTGGGCT 
GATGATGACG GGAACCCCAC AGAGCAGTAC CCTCTGAATC CCAATGGGTC CCCAGGGGGC 
GTGGCTGGCA TCTGCTCCTG TGATGGCCGC CACCTGGCTG TCATGCCTCA CCCTGAGCGG 
GCCGTTAGGC CTTGGCAGTG GGCATGGCGA CCCCCTCCAT TTGATACTCT GACCACCTCC 
CCCTGGCTCC AGCTCTTTAT CAATGCCCGA AACTGGACCC TGGAAGGGAG CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFAS ( XM_024450804.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306572.1 Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.

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CloneID	OHu31922
Clone ID Related Accession (Same CDS sequence)	XM_024450804.1 , NM_012393.3 , NM_012393.2
Accession Version	XM_024450804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phosphoribosylformylglycinamidine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCCAG TCCTTCACTT CTATGTTCGT CCCTCTGGCC ATGAGGGGGC AGCCCCTGGA 
CACACTCGGA GGAAACTGCA AGGGAAACTG CCAGAGCTGC AGGGCGTCGA GACTGAACTG 
TGCTACAACG TGAACTGGAC AGCTGAGGCC CTCCCCAGTG CTGAGGAGAC AAAGAAGCTG 
ATGTGGCTGT TTGGTTGCCC CTTACTGCTG GATGATGTTG CTCGGGAGTC CTGGCTCCTT 
CCTGGCTCCA ATGACCTGCT GCTGGAGGTC GGGCCCAGGC TGAACTTCTC CACCCCAACA 
TCCACCAACA TCGTGTCAGT GTGCCGCGCC ACTGGGCTGG GGCCTGTGGA TCGTGTGGAG 
ACCACCCGGC GCTACCGGCT CTCGTTTGCC CACCCCCCGT CAGCTGAGGT GGAAGCCATT 
GCTCTGGCTA CCCTGCACGA CCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGT 
TTCTCCCCTG AGAGCATGCC GGAACCCCTC AATGGCCCTA TCAATATACT GGGTGAGGGC 
CGGCTTGCGC TGGAGAAGGC CAACCAGGAG CTTGGTCTGG CTTTAGACTC TTGGGACCTA 
GACTTCTACA CCAAGCGCTT CCAGGAGCTA CAGCGGAACC CGAGCACTGT GGAGGCCTTT 
GACTTGGCGC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
GTGGATGGGC AGAAGCTGGT GCACTCACTG TTTGAGTCCA TCATGAGCAC CCAGGAATCC 
TCGAACCCCA ACAACGTCCT CAAATTCTGT GATAACAGCA GTGCAATCCA GGGAAAGGAA 
GTCCGATTCC TACGGCCTGA GGACCCCACA CGGCCAAGCC GCTTCCAGCA ACAGCAAGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACT CACAACTTTC CCACAGGAGT ATGCCCCTTT 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATTCGAGATG TCCAGTGCAC AGGCCGCGGG 
GCCCACGTGG TGGCTGGCAC TGCCGGCTAT TGCTTTGGAA ATCTGCATAT TCCAGGTTAC 
AATCTGCCCT GGGAGGATCC AAGCTTCCAG TATCCTGGGA ATTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AAGCCAGTAA TGGAGCTTCT GACTATGGCA ACAAGTTTGG GGAACCAGTG 
CTGGCTGGCT TCGCCCGCTC CTTGGGCCTC CAGCTCCCAG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGGGGCATT GGGTCCATGG AAGCTGACCA CATAAGCAAG 
GAGGCCCCAG AGCCAGGCAT GGAAGTTGTA AAGGTTGGAG GTCCCGTCTA CAGGATTGGA 
GTTGGAGGTG GAGCTGCTTC ATCTGTGCAG GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGAGG AGACCCGGAG ATGGAACAGA AGATGAACCG TGTGATCAGG 
GCTTGTGTGG AGGCCCCCAA GGGAAACCCC ATCTGCAGCC TTCATGATCA GGGCGCTGGT 
GGCAATGGCA ATGTCCTAAA AGAGCTGAGT GACCCAGCTG GAGCCATCAT TTACACCAGC 
CGCTTCCAGC TTGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC TGAGTACCAG 
GAATCAAATG CTCTTCTGCT GAGGTCCCCC AACCGGGACT TCCTGACTCA TGTCAGTGCC 
CGTGAACGTT GCCCGGCTTG CTTCGTGGGC ACCATCACTG GAGACCGGAG AATAGTGCTG 
GTGGACGATC GGGAGTGTCC TGTCAGAAGA AATGGCCAGG GGGATGCCCC CCCGACACCC 
CTGCCAACCC CTGTGGACCT GGAGCTCGAA TGGGTGCTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTGC AGAGGAAGCC CCCCATGCTG CAGCCTCTGG CCTTGCCCCC AGGGCTGAGC 
GTGCACCAGG CTCTGGAGAG GGTTCTGAGG CTGCCCGCCG TGGCCAGCAA GCGCTACCTC 
ACCAATAAGG TGGACCGCTC TGTGGGAGGC CTGGTGGCCC AGCAGCAGTG CGTGGGGCCC 
CTGCAAACTC CTCTGGCAGA TGTAGCGGTT GTGGCACTGA GCCATGAGGA GCTCATAGGG 
GCTGCCACAG CCTTGGGAGA ACAGCCAGTC AAGAGCCTGC TGGACCCAAA AGTCGCCGCC 
CGGCTGGCCG TGGCCGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC TGACCTCCGG 
GATGTGAAGT GTAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGCGCAGCT 
TTGGCGGATG CCTGTGAGGC TATGGTGGCA GTGATGGCAG CCCTGGGTGT GGCAGTGGAT 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCT CGGGTTGGCA CTGAGACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCCGTC TGCCCAGACA TCACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGAAGG GAGAGGCCAT CTGCTCTATG TGGCTCTGAG CCCTGGGCAG 
CACCGGCTCG GGGGCACAGC TCTGGCCCAG TGCTTCTCCC AGCTTGGGGA ACACCCTCCA 
GACCTGGACC TTCCTGAGAA CTTGGTGCGG GCCTTCAGCA TCACTCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCACGATGTC AGTGACGGAG GCCTCGTCAC ATGCCTGCTG 
GAGATGGCCT TTGCTGGAAA TTGCGGGCTA CAGGTGGATG TGCCTGTCCC CAGGGTTGAT 
GTCCTGTCTG TGCTGTTCGC TGAGGAGCCA GGCCTCGTGC TGGAGGTGCA GGAGCCAGAC 
CTGGCCCAGG TGCTGAAGCG TTACCGGGAT GCTGGCCTCC ATTGCCTGGA GCTGGGCCAC 
ACAGGCGAGG CCGGGCCCCA CGCCATGGTC CGGGTGTCAG TGAACGGGGC TGTGGTTCTG 
GAGGAGCCTG TTGGGGAGCT GCGAGCCCTC TGGGAGGAGA CGAGTTTCCA GCTGGACCGG 
CTACAGGCAG AGCCTCGCTG TGTGGCAGAG GAGGAACGGG GCCTGAGGGA GCGGATGGGG 
CCCAGCTATT GCCTGCCCCC CACCTTTCCC AAAGCCTCCG TGCCCCGTGA GCCTGGTGGT 
CCCAGCCCCC GAGTCGCCAT CTTGCGAGAG GAGGGCAGTA ATGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTAGCTGG GTTTGAGGTA TGGGACGTGA CCATGCAGGA CCTCTGCTCT 
GGGGCAATTG GGCTGGACAC TTTCCGTGGC GTGGCCTTCG TGGGCGGCTT CAGCTATGCA 
GATGTCCTGG GCTCTGCCAA AGGGTGGGCA GCTGCTGTGA CCTTTCATCC CAGGGCTGGG 
GCTGAGCTGA GGCGCTTCCG GAAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAATGGC 
TGTCAACTGC TGGCTCTGCT CGGCTGGGTG GGAGGCGACC CCAATGAGGA TGCTGCAGAG 
ATGGGCCCTG ACTCCCAGCC AGCCCGGCCA GGCCTTCTGC TACGCCACAA CCTGTCTGGG 
CGCTACGAGT CTCGCTGGGC CAGCGTGCGT GTGGGGCCTG GGCCAGCCCT GATGCTGCGA 
GGGATGGAGG GCGCCGTGCT GCCCGTGTGG AGTGCGCACG GGGAAGGTTA CGTAGCATTT 
TCTTCTCCGG AACTCCAAGC TCAGATTGAG GCCAGGGGCT TGGCTCCACT GCACTGGGCT 
GATGATGACG GGAACCCCAC AGAGCAGTAC CCTCTGAATC CCAATGGGTC CCCAGGGGGC 
GTGGCTGGCA TCTGCTCCTG TGATGGCCGC CACCTGGCTG TCATGCCTCA CCCTGAGCGG 
GCCGTTAGGC CTTGGCAGTG GGCATGGCGA CCCCCTCCAT TTGATACTCT GACCACCTCC 
CCCTGGCTCC AGCTCTTTAT CAATGCCCGA AACTGGACCC TGGAAGGGAG CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306572.1
CDS	341..4357
Translation	MSPVLHFYVRPSGHEGAAPGHTRRKLQGKLPELQGVETELCYNVNWTAEALPSAEETKKLMWLFGCPLLLDDVARESWLLPGSNDLLLEVGPRLNFSTPTSTNIVSVCRATGLGPVDRVETTRRYRLSFAHPPSAEVEAIALATLHDRMTEQHFPHPIQSFSPESMPEPLNGPINILGEGRLALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVEAFDLAQSNSEHSRHWFFKGQLHVDGQKLVHSLFESIMSTQESSNPNNVLKFCDNSSAIQGKEVRFLRPEDPTRPSRFQQQQGLRHVVFTAETHNFPTGVCPFSGATTGTGGRIRDVQCTGRGAHVVAGTAGYCFGNLHIPGYNLPWEDPSFQYPGNFARPLEVAIEASNGASDYGNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEADHISKEAPEPGMEVVKVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAPKGNPICSLHDQGAGGNGNVLKELSDPAGAIIYTSRFQLGDPTLNALEIWGAEYQESNALLLRSPNRDFLTHVSARERCPACFVGTITGDRRIVLVDDRECPVRRNGQGDAPPTPLPTPVDLELEWVLGKMPRKEFFLQRKPPMLQPLALPPGLSVHQALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVALSHEELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAVDGGKDSLSMAARVGTETVRAPGSLVISAYAVCPDITATVTPDLKHPEGRGHLLYVALSPGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFSITQGLLKDRLLCSGHDVSDGGLVTCLLEMAFAGNCGLQVDVPVPRVDVLSVLFAEEPGLVLEVQEPDLAQVLKRYRDAGLHCLELGHTGEAGPHAMVRVSVNGAVVLEEPVGELRALWEETSFQLDRLQAEPRCVAEEERGLRERMGPSYCLPPTFPKASVPREPGGPSPRVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPRAGAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGDPNEDAAEMGPDSQPARPGLLLRHNLSGRYESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYVAFSSPELQAQIEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSCDGRHLAVMPHPERAVRPWQWAWRPPPFDTLTTSPWLQLFINARNWTLEGSC

Target ORF information:

RefSeq Version	XM_024450804.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoribosylformylglycinamidine synthase (PFAS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450804.1

ORF Insert Sequence:

ATGTCCCCAG TCCTTCACTT CTATGTTCGT CCCTCTGGCC ATGAGGGGGC AGCCCCTGGA 
CACACTCGGA GGAAACTGCA AGGGAAACTG CCAGAGCTGC AGGGCGTCGA GACTGAACTG 
TGCTACAACG TGAACTGGAC AGCTGAGGCC CTCCCCAGTG CTGAGGAGAC AAAGAAGCTG 
ATGTGGCTGT TTGGTTGCCC CTTACTGCTG GATGATGTTG CTCGGGAGTC CTGGCTCCTT 
CCTGGCTCCA ATGACCTGCT GCTGGAGGTC GGGCCCAGGC TGAACTTCTC CACCCCAACA 
TCCACCAACA TCGTGTCAGT GTGCCGCGCC ACTGGGCTGG GGCCTGTGGA TCGTGTGGAG 
ACCACCCGGC GCTACCGGCT CTCGTTTGCC CACCCCCCGT CAGCTGAGGT GGAAGCCATT 
GCTCTGGCTA CCCTGCACGA CCGGATGACA GAGCAGCACT TCCCCCATCC CATCCAGAGT 
TTCTCCCCTG AGAGCATGCC GGAACCCCTC AATGGCCCTA TCAATATACT GGGTGAGGGC 
CGGCTTGCGC TGGAGAAGGC CAACCAGGAG CTTGGTCTGG CTTTAGACTC TTGGGACCTA 
GACTTCTACA CCAAGCGCTT CCAGGAGCTA CAGCGGAACC CGAGCACTGT GGAGGCCTTT 
GACTTGGCGC AGTCCAATAG CGAGCACAGC CGACACTGGT TCTTCAAGGG CCAGCTCCAC 
GTGGATGGGC AGAAGCTGGT GCACTCACTG TTTGAGTCCA TCATGAGCAC CCAGGAATCC 
TCGAACCCCA ACAACGTCCT CAAATTCTGT GATAACAGCA GTGCAATCCA GGGAAAGGAA 
GTCCGATTCC TACGGCCTGA GGACCCCACA CGGCCAAGCC GCTTCCAGCA ACAGCAAGGG 
CTGAGACATG TTGTCTTCAC AGCAGAGACT CACAACTTTC CCACAGGAGT ATGCCCCTTT 
AGTGGTGCAA CCACTGGCAC AGGGGGCCGG ATTCGAGATG TCCAGTGCAC AGGCCGCGGG 
GCCCACGTGG TGGCTGGCAC TGCCGGCTAT TGCTTTGGAA ATCTGCATAT TCCAGGTTAC 
AATCTGCCCT GGGAGGATCC AAGCTTCCAG TATCCTGGGA ATTTTGCCCG GCCCCTGGAG 
GTTGCCATTG AAGCCAGTAA TGGAGCTTCT GACTATGGCA ACAAGTTTGG GGAACCAGTG 
CTGGCTGGCT TCGCCCGCTC CTTGGGCCTC CAGCTCCCAG ACGGCCAGCG GCGTGAGTGG 
ATCAAGCCCA TCATGTTTAG TGGGGGCATT GGGTCCATGG AAGCTGACCA CATAAGCAAG 
GAGGCCCCAG AGCCAGGCAT GGAAGTTGTA AAGGTTGGAG GTCCCGTCTA CAGGATTGGA 
GTTGGAGGTG GAGCTGCTTC ATCTGTGCAG GTGCAGGGAG ATAACACCAG TGACCTGGAC 
TTTGGGGCTG TGCAGCGAGG AGACCCGGAG ATGGAACAGA AGATGAACCG TGTGATCAGG 
GCTTGTGTGG AGGCCCCCAA GGGAAACCCC ATCTGCAGCC TTCATGATCA GGGCGCTGGT 
GGCAATGGCA ATGTCCTAAA AGAGCTGAGT GACCCAGCTG GAGCCATCAT TTACACCAGC 
CGCTTCCAGC TTGGGGACCC AACCCTGAAT GCCCTGGAAA TCTGGGGGGC TGAGTACCAG 
GAATCAAATG CTCTTCTGCT GAGGTCCCCC AACCGGGACT TCCTGACTCA TGTCAGTGCC 
CGTGAACGTT GCCCGGCTTG CTTCGTGGGC ACCATCACTG GAGACCGGAG AATAGTGCTG 
GTGGACGATC GGGAGTGTCC TGTCAGAAGA AATGGCCAGG GGGATGCCCC CCCGACACCC 
CTGCCAACCC CTGTGGACCT GGAGCTCGAA TGGGTGCTGG GCAAGATGCC TCGGAAGGAG 
TTCTTCCTGC AGAGGAAGCC CCCCATGCTG CAGCCTCTGG CCTTGCCCCC AGGGCTGAGC 
GTGCACCAGG CTCTGGAGAG GGTTCTGAGG CTGCCCGCCG TGGCCAGCAA GCGCTACCTC 
ACCAATAAGG TGGACCGCTC TGTGGGAGGC CTGGTGGCCC AGCAGCAGTG CGTGGGGCCC 
CTGCAAACTC CTCTGGCAGA TGTAGCGGTT GTGGCACTGA GCCATGAGGA GCTCATAGGG 
GCTGCCACAG CCTTGGGAGA ACAGCCAGTC AAGAGCCTGC TGGACCCAAA AGTCGCCGCC 
CGGCTGGCCG TGGCCGAAGC CCTCACCAAC CTGGTGTTTG CTCTGGTCAC TGACCTCCGG 
GATGTGAAGT GTAGCGGGAA CTGGATGTGG GCAGCCAAGC TCCCAGGGGA GGGCGCAGCT 
TTGGCGGATG CCTGTGAGGC TATGGTGGCA GTGATGGCAG CCCTGGGTGT GGCAGTGGAT 
GGTGGCAAGG ACTCCCTCAG CATGGCTGCT CGGGTTGGCA CTGAGACCGT GCGGGCTCCT 
GGGTCACTGG TCATCTCAGC CTATGCCGTC TGCCCAGACA TCACAGCCAC TGTGACCCCA 
GACCTCAAGC ATCCTGAAGG GAGAGGCCAT CTGCTCTATG TGGCTCTGAG CCCTGGGCAG 
CACCGGCTCG GGGGCACAGC TCTGGCCCAG TGCTTCTCCC AGCTTGGGGA ACACCCTCCA 
GACCTGGACC TTCCTGAGAA CTTGGTGCGG GCCTTCAGCA TCACTCAGGG GCTGCTGAAA 
GACCGCCTCC TCTGCTCAGG CCACGATGTC AGTGACGGAG GCCTCGTCAC ATGCCTGCTG 
GAGATGGCCT TTGCTGGAAA TTGCGGGCTA CAGGTGGATG TGCCTGTCCC CAGGGTTGAT 
GTCCTGTCTG TGCTGTTCGC TGAGGAGCCA GGCCTCGTGC TGGAGGTGCA GGAGCCAGAC 
CTGGCCCAGG TGCTGAAGCG TTACCGGGAT GCTGGCCTCC ATTGCCTGGA GCTGGGCCAC 
ACAGGCGAGG CCGGGCCCCA CGCCATGGTC CGGGTGTCAG TGAACGGGGC TGTGGTTCTG 
GAGGAGCCTG TTGGGGAGCT GCGAGCCCTC TGGGAGGAGA CGAGTTTCCA GCTGGACCGG 
CTACAGGCAG AGCCTCGCTG TGTGGCAGAG GAGGAACGGG GCCTGAGGGA GCGGATGGGG 
CCCAGCTATT GCCTGCCCCC CACCTTTCCC AAAGCCTCCG TGCCCCGTGA GCCTGGTGGT 
CCCAGCCCCC GAGTCGCCAT CTTGCGAGAG GAGGGCAGTA ATGGAGACCG GGAGATGGCC 
GATGCCTTCC ACTTAGCTGG GTTTGAGGTA TGGGACGTGA CCATGCAGGA CCTCTGCTCT 
GGGGCAATTG GGCTGGACAC TTTCCGTGGC GTGGCCTTCG TGGGCGGCTT CAGCTATGCA 
GATGTCCTGG GCTCTGCCAA AGGGTGGGCA GCTGCTGTGA CCTTTCATCC CAGGGCTGGG 
GCTGAGCTGA GGCGCTTCCG GAAGCGGCCA GACACCTTCA GCCTGGGCGT GTGTAATGGC 
TGTCAACTGC TGGCTCTGCT CGGCTGGGTG GGAGGCGACC CCAATGAGGA TGCTGCAGAG 
ATGGGCCCTG ACTCCCAGCC AGCCCGGCCA GGCCTTCTGC TACGCCACAA CCTGTCTGGG 
CGCTACGAGT CTCGCTGGGC CAGCGTGCGT GTGGGGCCTG GGCCAGCCCT GATGCTGCGA 
GGGATGGAGG GCGCCGTGCT GCCCGTGTGG AGTGCGCACG GGGAAGGTTA CGTAGCATTT 
TCTTCTCCGG AACTCCAAGC TCAGATTGAG GCCAGGGGCT TGGCTCCACT GCACTGGGCT 
GATGATGACG GGAACCCCAC AGAGCAGTAC CCTCTGAATC CCAATGGGTC CCCAGGGGGC 
GTGGCTGGCA TCTGCTCCTG TGATGGCCGC CACCTGGCTG TCATGCCTCA CCCTGAGCGG 
GCCGTTAGGC CTTGGCAGTG GGCATGGCGA CCCCCTCCAT TTGATACTCT GACCACCTCC 
CCCTGGCTCC AGCTCTTTAT CAATGCCCGA AACTGGACCC TGGAAGGGAG CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of the purine biosynthetic enzyme phosphoribosyl formylglycinamidine synthase (FGAMS) in neurons.
Journal of neurochemistry(2018 Jan)
Mangold CA,Yao PJ,Du M,Freeman WM,Benkovic SJ,Szpara ML

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Nature442(7099)199-202(2006 Jul)
Girard A,Sachidanandam R,Hannon GJ,Carmell MA

Human phosphoribosylformylglycineamide amidotransferase (FGARAT): regional mapping, complete coding sequence, isolation of a functional genomic clone, and DNA sequence analysis.
Gene239(2)381-91(1999 Nov)
Patterson D,Bleskan J,Gardiner K,Bowersox J

The human GARS-AIRS-GART gene encodes two proteins which are differentially expressed during human brain development and temporally overexpressed in cerebellum of individuals with Down syndrome.
Human molecular genetics6(12)2043-50(1997 Nov)
Brodsky G,Barnes T,Bleskan J,Becker L,Cox M,Patterson D

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PFAS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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