PCF11 cDNA ORF clone, Homo sapiens(human)

The following PCF11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCF11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu29064 NM_015885.4
Latest version!
Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu100712 NM_001346415.2
Latest version!
Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu45367 NM_001346413.2
Latest version!
Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu45368 NM_001346414.2
Latest version!
Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
View Old Accession Versions >>
OHu45367 NM_001346413.1 Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu100712 NM_001346415.1 Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
OHu45368 NM_001346414.1 Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu29064 NM_015885.3 Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu29064
    Clone ID Related Accession (Same CDS sequence) NM_015885.3 , NM_015885.4
    Accession Version NM_015885.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-22
    Organism Homo sapiens(human)
    Product pre-mRNA cleavage complex 2 protein Pcf11 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB053362.1 and BC146778.1. This sequence is a reference standard in the RefSeqGene project. On Sep 22, 2007 this sequence version replaced NM_015885.2. Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC146778.1, SRR1803615.79390.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA
    GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT
    TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG
    CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA
    GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT
    GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA
    GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA
    TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA
    GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA
    GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA
    CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT
    GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT
    CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA
    ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT
    GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT
    AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC
    CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT
    GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT
    GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT
    CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA
    CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG
    CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT
    TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC
    AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT
    TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT
    CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT
    GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG
    GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT
    CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT
    GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA
    ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACAAG TAAGTGAAGT AACTGCTCAG
    CCTCCCCCTG AAGAGGAGGA AGATCAAAAT GAAGATCAAG ATGTTCCAGA TCTTACTAAT
    TTTACAGTTG AAGAATTGAA ACAACGTTAT GACAGTGTTA TAAATCGACT GTACACTGGT
    ATTCAGTGTT ACTCTTGTGG AATGAGGTTT ACAACATCAC AGACAGATGT TTATGCAGAT
    CATTTGGACT GGCATTATCG GCAAAATAGA ACTGAAAAGG ATGTTAGCCG AAAAGTCACT
    CATAGACGTT GGTACTACAG TTTAACAGAC TGGATAGAAT TTGAGGAGAT AGCTGATCTG
    GAAGAACGGG CAAAGAGCCA GTTTTTTGAA AAGGTGCATG AAGAAGTTGT GCTCAAAACT
    CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA AGTGTACCTG CTGGACCAGC TGGAGCAGTT
    GAGAGTTGTG AAATCTGTCA AGAACAATTT GAACAATACT GGGATGAAGA AGAGGAGGAA
    TGGCATTTGA AAAATGCTAT TAGAGTAGAT GGAAAGATTT ATCATCCATC ATGTTATGAA
    GATTATCAAA ATACATCTTC ATTTGATTGT ACACCATCTC CCAGCAAGAC ACCAGTTGAA
    AACCCCTTGA ATATTATGTT GAACATTGTC AAAAACGAAT TGCAGGAACC CTGTGACAGT
    CCCAAAGTTA AGGAAGAACG AATTGATACA CCACCAGCTT GTACAGAGGA AAGCATAGCA
    ACACCCTCTG AAATTAAAAC AGAAAATGAC ACAGTCGAGT CAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056969.2
    CDS346..5013
    Translation

    Target ORF information:

    RefSeq Version NM_015885.3
    Organism Homo sapiens(human)
    Definition Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015885.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA
    GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT
    TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG
    CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA
    GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT
    GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA
    GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA
    TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA
    GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA
    GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA
    CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT
    GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT
    CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA
    ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT
    GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT
    AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC
    CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT
    GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT
    GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT
    CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA
    CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG
    CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT
    TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC
    AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT
    TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT
    CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT
    GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG
    GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT
    CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT
    GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA
    ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACAAG TAAGTGAAGT AACTGCTCAG
    CCTCCCCCTG AAGAGGAGGA AGATCAAAAT GAAGATCAAG ATGTTCCAGA TCTTACTAAT
    TTTACAGTTG AAGAATTGAA ACAACGTTAT GACAGTGTTA TAAATCGACT GTACACTGGT
    ATTCAGTGTT ACTCTTGTGG AATGAGGTTT ACAACATCAC AGACAGATGT TTATGCAGAT
    CATTTGGACT GGCATTATCG GCAAAATAGA ACTGAAAAGG ATGTTAGCCG AAAAGTCACT
    CATAGACGTT GGTACTACAG TTTAACAGAC TGGATAGAAT TTGAGGAGAT AGCTGATCTG
    GAAGAACGGG CAAAGAGCCA GTTTTTTGAA AAGGTGCATG AAGAAGTTGT GCTCAAAACT
    CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA AGTGTACCTG CTGGACCAGC TGGAGCAGTT
    GAGAGTTGTG AAATCTGTCA AGAACAATTT GAACAATACT GGGATGAAGA AGAGGAGGAA
    TGGCATTTGA AAAATGCTAT TAGAGTAGAT GGAAAGATTT ATCATCCATC ATGTTATGAA
    GATTATCAAA ATACATCTTC ATTTGATTGT ACACCATCTC CCAGCAAGAC ACCAGTTGAA
    AACCCCTTGA ATATTATGTT GAACATTGTC AAAAACGAAT TGCAGGAACC CTGTGACAGT
    CCCAAAGTTA AGGAAGAACG AATTGATACA CCACCAGCTT GTACAGAGGA AAGCATAGCA
    ACACCCTCTG AAATTAAAAC AGAAAATGAC ACAGTCGAGT CAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu29064
    Clone ID Related Accession (Same CDS sequence) NM_015885.3 , NM_015885.4
    Accession Version NM_015885.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product pre-mRNA cleavage complex 2 protein Pcf11 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB053362.1, BC146778.1 and AP000873.5. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_015885.3. Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC146778.1, AB020631.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA
    GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT
    TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG
    CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA
    GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT
    GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA
    GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA
    TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA
    GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA
    GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA
    CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT
    GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT
    CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA
    ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT
    GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT
    AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC
    CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT
    GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT
    GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT
    CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA
    CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG
    CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT
    TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC
    AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT
    TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT
    CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT
    GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG
    GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT
    CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT
    GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA
    ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACAAG TAAGTGAAGT AACTGCTCAG
    CCTCCCCCTG AAGAGGAGGA AGATCAAAAT GAAGATCAAG ATGTTCCAGA TCTTACTAAT
    TTTACAGTTG AAGAATTGAA ACAACGTTAT GACAGTGTTA TAAATCGACT GTACACTGGT
    ATTCAGTGTT ACTCTTGTGG AATGAGGTTT ACAACATCAC AGACAGATGT TTATGCAGAT
    CATTTGGACT GGCATTATCG GCAAAATAGA ACTGAAAAGG ATGTTAGCCG AAAAGTCACT
    CATAGACGTT GGTACTACAG TTTAACAGAC TGGATAGAAT TTGAGGAGAT AGCTGATCTG
    GAAGAACGGG CAAAGAGCCA GTTTTTTGAA AAGGTGCATG AAGAAGTTGT GCTCAAAACT
    CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA AGTGTACCTG CTGGACCAGC TGGAGCAGTT
    GAGAGTTGTG AAATCTGTCA AGAACAATTT GAACAATACT GGGATGAAGA AGAGGAGGAA
    TGGCATTTGA AAAATGCTAT TAGAGTAGAT GGAAAGATTT ATCATCCATC ATGTTATGAA
    GATTATCAAA ATACATCTTC ATTTGATTGT ACACCATCTC CCAGCAAGAC ACCAGTTGAA
    AACCCCTTGA ATATTATGTT GAACATTGTC AAAAACGAAT TGCAGGAACC CTGTGACAGT
    CCCAAAGTTA AGGAAGAACG AATTGATACA CCACCAGCTT GTACAGAGGA AAGCATAGCA
    ACACCCTCTG AAATTAAAAC AGAAAATGAC ACAGTCGAGT CAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056969.2
    CDS310..4977
    Translation

    Target ORF information:

    RefSeq Version NM_015885.4
    Organism Homo sapiens(human)
    Definition Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015885.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA
    GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT
    TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG
    CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA
    GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT
    GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA
    GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA
    TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA
    GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA
    GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA
    CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT
    GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT
    CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA
    ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT
    GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT
    AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC
    CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT
    GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT
    GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT
    CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA
    CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG
    CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT
    TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC
    AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT
    TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT
    CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT
    GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG
    GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT
    CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT
    GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA
    ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACAAG TAAGTGAAGT AACTGCTCAG
    CCTCCCCCTG AAGAGGAGGA AGATCAAAAT GAAGATCAAG ATGTTCCAGA TCTTACTAAT
    TTTACAGTTG AAGAATTGAA ACAACGTTAT GACAGTGTTA TAAATCGACT GTACACTGGT
    ATTCAGTGTT ACTCTTGTGG AATGAGGTTT ACAACATCAC AGACAGATGT TTATGCAGAT
    CATTTGGACT GGCATTATCG GCAAAATAGA ACTGAAAAGG ATGTTAGCCG AAAAGTCACT
    CATAGACGTT GGTACTACAG TTTAACAGAC TGGATAGAAT TTGAGGAGAT AGCTGATCTG
    GAAGAACGGG CAAAGAGCCA GTTTTTTGAA AAGGTGCATG AAGAAGTTGT GCTCAAAACT
    CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA AGTGTACCTG CTGGACCAGC TGGAGCAGTT
    GAGAGTTGTG AAATCTGTCA AGAACAATTT GAACAATACT GGGATGAAGA AGAGGAGGAA
    TGGCATTTGA AAAATGCTAT TAGAGTAGAT GGAAAGATTT ATCATCCATC ATGTTATGAA
    GATTATCAAA ATACATCTTC ATTTGATTGT ACACCATCTC CCAGCAAGAC ACCAGTTGAA
    AACCCCTTGA ATATTATGTT GAACATTGTC AAAAACGAAT TGCAGGAACC CTGTGACAGT
    CCCAAAGTTA AGGAAGAACG AATTGATACA CCACCAGCTT GTACAGAGGA AAGCATAGCA
    ACACCCTCTG AAATTAAAAC AGAAAATGAC ACAGTCGAGT CAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100712
    Clone ID Related Accession (Same CDS sequence) NM_001346415.1 , NM_001346415.2
    Accession Version NM_001346415.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-22
    Organism Homo sapiens(human)
    Product pre-mRNA cleavage complex 2 protein Pcf11 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000873.5. On Oct 9, 2016 this sequence version replaced XM_017017918.1. Transcript Variant: This variant (4) uses two alternate in-frame splice junctions compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.233378.1, SRR1803611.192824.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA
    GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT
    TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG
    CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA
    GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT
    GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA
    GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA
    TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA
    GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA
    GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA
    CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT
    GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT
    CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA
    ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT
    GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT
    AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC
    CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT
    GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT
    GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT
    CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA
    CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG
    CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT
    TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC
    AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT
    TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT
    CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT
    GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG
    GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT
    CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT
    GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA
    ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACTAA GTGAAGTAAC TGCTCAGCCT
    CCCCCTGAAG AGGAGGAAGA TCAAAATGAA GATCAAGATG TTCCAGATCT TACTAATTTT
    ACAGTTGAAG AATTGAAACA ACGTTATGAC AGTGTTATAA ATCGACTGTA CACTGGTATT
    CAGTGTTACT CTTGTGGAAT GAGGTTTACA ACATCACAGA CAGATGTTTA TGCAGATCAT
    TTGGACTGGC ATTATCGGCA AAATAGAACT GAAAAGGATG TTAGCCGAAA AGTCACTCAT
    AGACGTTGGT ACTACAGTTT AACAGACTGG ATAGAATTTG AGGAGATAGC TGATCTGGAA
    GAACGGGCAA AGAGCCAGTT TTTTGAAAAG GTGCATGAAG AAGTTGTGCT CAAAACTCAA
    GAGGCTGCTA AAGAAAAAGA GTTCCAAAGT GTACCTGCTG GACCAGCTGG AGCAGTTGAG
    AGTTGTGAAA TCTGTCAAGA ACAATTTGAA CAATACTGGG ATGAAGAAGA GGAGGAATGG
    CATTTGAAAA ATGCTATTAG AGTAGATGGA AAGATTTATC ATCCATCATG TTATGAAGAT
    TATCAAAATA CATCTTCATT TGATTGTACA CCATCTCCCA GCAAGACACC AGTTGAAAAC
    CCCTTGAATA TTATGTTGAA CATTGTCAAA AACGAATTGC AGGAACCCTG TGACAGTCCC
    AAAGTTAAGG AAGAACGAAT TGATACACCA CCAGCTTGTA CAGAGGAAAG CATAGCAACA
    CCCTCTGAAA TTAAAACAGA AAATGACACA GTCGAGTCAG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333344.1
    CDS346..5010
    Translation

    Target ORF information:

    RefSeq Version NM_001346415.1
    Organism Homo sapiens(human)
    Definition Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346415.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA
    GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT
    TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG
    CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA
    GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT
    GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA
    GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA
    TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA
    GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA
    GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA
    CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT
    GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT
    CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA
    ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT
    GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT
    AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC
    CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT
    GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT
    GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT
    CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA
    CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG
    CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT
    TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC
    AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT
    TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT
    CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT
    GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG
    GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT
    CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT
    GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA
    ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACTAA GTGAAGTAAC TGCTCAGCCT
    CCCCCTGAAG AGGAGGAAGA TCAAAATGAA GATCAAGATG TTCCAGATCT TACTAATTTT
    ACAGTTGAAG AATTGAAACA ACGTTATGAC AGTGTTATAA ATCGACTGTA CACTGGTATT
    CAGTGTTACT CTTGTGGAAT GAGGTTTACA ACATCACAGA CAGATGTTTA TGCAGATCAT
    TTGGACTGGC ATTATCGGCA AAATAGAACT GAAAAGGATG TTAGCCGAAA AGTCACTCAT
    AGACGTTGGT ACTACAGTTT AACAGACTGG ATAGAATTTG AGGAGATAGC TGATCTGGAA
    GAACGGGCAA AGAGCCAGTT TTTTGAAAAG GTGCATGAAG AAGTTGTGCT CAAAACTCAA
    GAGGCTGCTA AAGAAAAAGA GTTCCAAAGT GTACCTGCTG GACCAGCTGG AGCAGTTGAG
    AGTTGTGAAA TCTGTCAAGA ACAATTTGAA CAATACTGGG ATGAAGAAGA GGAGGAATGG
    CATTTGAAAA ATGCTATTAG AGTAGATGGA AAGATTTATC ATCCATCATG TTATGAAGAT
    TATCAAAATA CATCTTCATT TGATTGTACA CCATCTCCCA GCAAGACACC AGTTGAAAAC
    CCCTTGAATA TTATGTTGAA CATTGTCAAA AACGAATTGC AGGAACCCTG TGACAGTCCC
    AAAGTTAAGG AAGAACGAAT TGATACACCA CCAGCTTGTA CAGAGGAAAG CATAGCAACA
    CCCTCTGAAA TTAAAACAGA AAATGACACA GTCGAGTCAG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu100712
    Clone ID Related Accession (Same CDS sequence) NM_001346415.1 , NM_001346415.2
    Accession Version NM_001346415.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-22
    Organism Homo sapiens(human)
    Product pre-mRNA cleavage complex 2 protein Pcf11 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000873.5. On Jun 2, 2019 this sequence version replaced NM_001346415.1. Transcript Variant: This variant (4) uses two alternate in-frame splice junctions compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.233378.1, SRR1803611.192824.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA
    GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT
    TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG
    CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA
    GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT
    GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA
    GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA
    TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA
    GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA
    GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA
    CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT
    GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT
    CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA
    ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT
    GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT
    AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC
    CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT
    GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT
    GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT
    CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA
    CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG
    CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT
    TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC
    AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT
    TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT
    CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT
    GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG
    GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT
    CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT
    GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA
    ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACTAA GTGAAGTAAC TGCTCAGCCT
    CCCCCTGAAG AGGAGGAAGA TCAAAATGAA GATCAAGATG TTCCAGATCT TACTAATTTT
    ACAGTTGAAG AATTGAAACA ACGTTATGAC AGTGTTATAA ATCGACTGTA CACTGGTATT
    CAGTGTTACT CTTGTGGAAT GAGGTTTACA ACATCACAGA CAGATGTTTA TGCAGATCAT
    TTGGACTGGC ATTATCGGCA AAATAGAACT GAAAAGGATG TTAGCCGAAA AGTCACTCAT
    AGACGTTGGT ACTACAGTTT AACAGACTGG ATAGAATTTG AGGAGATAGC TGATCTGGAA
    GAACGGGCAA AGAGCCAGTT TTTTGAAAAG GTGCATGAAG AAGTTGTGCT CAAAACTCAA
    GAGGCTGCTA AAGAAAAAGA GTTCCAAAGT GTACCTGCTG GACCAGCTGG AGCAGTTGAG
    AGTTGTGAAA TCTGTCAAGA ACAATTTGAA CAATACTGGG ATGAAGAAGA GGAGGAATGG
    CATTTGAAAA ATGCTATTAG AGTAGATGGA AAGATTTATC ATCCATCATG TTATGAAGAT
    TATCAAAATA CATCTTCATT TGATTGTACA CCATCTCCCA GCAAGACACC AGTTGAAAAC
    CCCTTGAATA TTATGTTGAA CATTGTCAAA AACGAATTGC AGGAACCCTG TGACAGTCCC
    AAAGTTAAGG AAGAACGAAT TGATACACCA CCAGCTTGTA CAGAGGAAAG CATAGCAACA
    CCCTCTGAAA TTAAAACAGA AAATGACACA GTCGAGTCAG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333344.1
    CDS310..4974
    Translation

    Target ORF information:

    RefSeq Version NM_001346415.2
    Organism Homo sapiens(human)
    Definition Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346415.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA
    GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT
    TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG
    CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA
    GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT
    GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA
    GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA
    TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA
    GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA
    GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA
    CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT
    GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT
    CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA
    ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT
    GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT
    AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC
    CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT
    GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT
    GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT
    CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA
    CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG
    CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT
    TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC
    AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT
    TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT
    CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT
    GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG
    GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT
    CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT
    GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA
    ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACTAA GTGAAGTAAC TGCTCAGCCT
    CCCCCTGAAG AGGAGGAAGA TCAAAATGAA GATCAAGATG TTCCAGATCT TACTAATTTT
    ACAGTTGAAG AATTGAAACA ACGTTATGAC AGTGTTATAA ATCGACTGTA CACTGGTATT
    CAGTGTTACT CTTGTGGAAT GAGGTTTACA ACATCACAGA CAGATGTTTA TGCAGATCAT
    TTGGACTGGC ATTATCGGCA AAATAGAACT GAAAAGGATG TTAGCCGAAA AGTCACTCAT
    AGACGTTGGT ACTACAGTTT AACAGACTGG ATAGAATTTG AGGAGATAGC TGATCTGGAA
    GAACGGGCAA AGAGCCAGTT TTTTGAAAAG GTGCATGAAG AAGTTGTGCT CAAAACTCAA
    GAGGCTGCTA AAGAAAAAGA GTTCCAAAGT GTACCTGCTG GACCAGCTGG AGCAGTTGAG
    AGTTGTGAAA TCTGTCAAGA ACAATTTGAA CAATACTGGG ATGAAGAAGA GGAGGAATGG
    CATTTGAAAA ATGCTATTAG AGTAGATGGA AAGATTTATC ATCCATCATG TTATGAAGAT
    TATCAAAATA CATCTTCATT TGATTGTACA CCATCTCCCA GCAAGACACC AGTTGAAAAC
    CCCTTGAATA TTATGTTGAA CATTGTCAAA AACGAATTGC AGGAACCCTG TGACAGTCCC
    AAAGTTAAGG AAGAACGAAT TGATACACCA CCAGCTTGTA CAGAGGAAAG CATAGCAACA
    CCCTCTGAAA TTAAAACAGA AAATGACACA GTCGAGTCAG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45367
    Clone ID Related Accession (Same CDS sequence) NM_001346413.2 , NM_001346413.1
    Accession Version NM_001346413.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5061bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-22
    Organism Homo sapiens(human)
    Product pre-mRNA cleavage complex 2 protein Pcf11 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000873.5. On Jun 2, 2019 this sequence version replaced NM_001346413.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.232134.1, SRR1803616.265971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT
    AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT
    CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG
    GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG
    ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG
    AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT
    CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA
    CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT
    CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA
    GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA
    CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT
    TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT
    CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA
    CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG
    ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA
    GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA
    GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA
    AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT
    CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG
    TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG
    CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT
    GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA
    GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG
    GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA
    AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG
    CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG
    CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT
    GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT
    GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC
    AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA
    CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA
    CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT
    TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA
    ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA
    GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG
    AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA
    AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA
    GCTACAACAC AAGTAAGTGA AGTAACTGCT CAGCCTCCCC CTGAAGAGGA GGAAGATCAA
    AATGAAGATC AAGATGTTCC AGATCTTACT AATTTTACAG TTGAAGAATT GAAACAACGT
    TATGACAGTG TTATAAATCG ACTGTACACT GGTATTCAGT GTTACTCTTG TGGAATGAGG
    TTTACAACAT CACAGACAGA TGTTTATGCA GATCATTTGG ACTGGCATTA TCGGCAAAAT
    AGAACTGAAA AGGATGTTAG CCGAAAAGTC ACTCATAGAC GTTGGTACTA CAGTTTAACA
    GACTGGATAG AATTTGAGGA GATAGCTGAT CTGGAAGAAC GGGCAAAGAG CCAGTTTTTT
    GAAAAGGTGC ATGAAGAAGT TGTGCTCAAA ACTCAAGAGG CTGCTAAAGA AAAAGAGTTC
    CAAAGTGTAC CTGCTGGACC AGCTGGAGCA GTTGAGAGTT GTGAAATCTG TCAAGAACAA
    TTTGAACAAT ACTGGGATGA AGAAGAGGAG GAATGGCATT TGAAAAATGC TATTAGAGTA
    GATGGAAAGA TTTATCATCC ATCATGTTAT GAAGATTATC AAAATACATC TTCATTTGAT
    TGTACACCAT CTCCCAGCAA GACACCAGTT GAAAACCCCT TGAATATTAT GTTGAACATT
    GTCAAAAACG AATTGCAGGA ACCCTGTGAC AGTCCCAAAG TTAAGGAAGA ACGAATTGAT
    ACACCACCAG CTTGTACAGA GGAAAGCATA GCAACACCCT CTGAAATTAA AACAGAAAAT
    GACACAGTCG AGTCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333342.1
    CDS310..5370
    Translation

    Target ORF information:

    RefSeq Version NM_001346413.2
    Organism Homo sapiens(human)
    Definition Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346413.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT
    AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT
    CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG
    GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG
    ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG
    AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT
    CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA
    CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT
    CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA
    GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA
    CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT
    TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT
    CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA
    CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG
    ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA
    GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA
    GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA
    AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT
    CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG
    TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG
    CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT
    GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA
    GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG
    GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA
    AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG
    CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG
    CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT
    GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT
    GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC
    AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA
    CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA
    CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT
    TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA
    ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA
    GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG
    AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA
    AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA
    GCTACAACAC AAGTAAGTGA AGTAACTGCT CAGCCTCCCC CTGAAGAGGA GGAAGATCAA
    AATGAAGATC AAGATGTTCC AGATCTTACT AATTTTACAG TTGAAGAATT GAAACAACGT
    TATGACAGTG TTATAAATCG ACTGTACACT GGTATTCAGT GTTACTCTTG TGGAATGAGG
    TTTACAACAT CACAGACAGA TGTTTATGCA GATCATTTGG ACTGGCATTA TCGGCAAAAT
    AGAACTGAAA AGGATGTTAG CCGAAAAGTC ACTCATAGAC GTTGGTACTA CAGTTTAACA
    GACTGGATAG AATTTGAGGA GATAGCTGAT CTGGAAGAAC GGGCAAAGAG CCAGTTTTTT
    GAAAAGGTGC ATGAAGAAGT TGTGCTCAAA ACTCAAGAGG CTGCTAAAGA AAAAGAGTTC
    CAAAGTGTAC CTGCTGGACC AGCTGGAGCA GTTGAGAGTT GTGAAATCTG TCAAGAACAA
    TTTGAACAAT ACTGGGATGA AGAAGAGGAG GAATGGCATT TGAAAAATGC TATTAGAGTA
    GATGGAAAGA TTTATCATCC ATCATGTTAT GAAGATTATC AAAATACATC TTCATTTGAT
    TGTACACCAT CTCCCAGCAA GACACCAGTT GAAAACCCCT TGAATATTAT GTTGAACATT
    GTCAAAAACG AATTGCAGGA ACCCTGTGAC AGTCCCAAAG TTAAGGAAGA ACGAATTGAT
    ACACCACCAG CTTGTACAGA GGAAAGCATA GCAACACCCT CTGAAATTAA AACAGAAAAT
    GACACAGTCG AGTCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45367
    Clone ID Related Accession (Same CDS sequence) NM_001346413.2 , NM_001346413.1
    Accession Version NM_001346413.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5061bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-22
    Organism Homo sapiens(human)
    Product pre-mRNA cleavage complex 2 protein Pcf11 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000873.5. On Oct 8, 2016 this sequence version replaced XM_005274048.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.265971.1, SRR1803611.232134.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT
    AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT
    CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG
    GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG
    ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG
    AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT
    CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA
    CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT
    CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA
    GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA
    CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT
    TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT
    CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA
    CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG
    ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA
    GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA
    GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA
    AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT
    CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG
    TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG
    CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT
    GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA
    GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG
    GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA
    AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG
    CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG
    CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT
    GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT
    GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC
    AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA
    CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA
    CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT
    TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA
    ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA
    GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG
    AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA
    AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA
    GCTACAACAC AAGTAAGTGA AGTAACTGCT CAGCCTCCCC CTGAAGAGGA GGAAGATCAA
    AATGAAGATC AAGATGTTCC AGATCTTACT AATTTTACAG TTGAAGAATT GAAACAACGT
    TATGACAGTG TTATAAATCG ACTGTACACT GGTATTCAGT GTTACTCTTG TGGAATGAGG
    TTTACAACAT CACAGACAGA TGTTTATGCA GATCATTTGG ACTGGCATTA TCGGCAAAAT
    AGAACTGAAA AGGATGTTAG CCGAAAAGTC ACTCATAGAC GTTGGTACTA CAGTTTAACA
    GACTGGATAG AATTTGAGGA GATAGCTGAT CTGGAAGAAC GGGCAAAGAG CCAGTTTTTT
    GAAAAGGTGC ATGAAGAAGT TGTGCTCAAA ACTCAAGAGG CTGCTAAAGA AAAAGAGTTC
    CAAAGTGTAC CTGCTGGACC AGCTGGAGCA GTTGAGAGTT GTGAAATCTG TCAAGAACAA
    TTTGAACAAT ACTGGGATGA AGAAGAGGAG GAATGGCATT TGAAAAATGC TATTAGAGTA
    GATGGAAAGA TTTATCATCC ATCATGTTAT GAAGATTATC AAAATACATC TTCATTTGAT
    TGTACACCAT CTCCCAGCAA GACACCAGTT GAAAACCCCT TGAATATTAT GTTGAACATT
    GTCAAAAACG AATTGCAGGA ACCCTGTGAC AGTCCCAAAG TTAAGGAAGA ACGAATTGAT
    ACACCACCAG CTTGTACAGA GGAAAGCATA GCAACACCCT CTGAAATTAA AACAGAAAAT
    GACACAGTCG AGTCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333342.1
    CDS346..5406
    Translation

    Target ORF information:

    RefSeq Version NM_001346413.1
    Organism Homo sapiens(human)
    Definition Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346413.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT
    AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT
    CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG
    GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG
    ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG
    AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT
    CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA
    CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT
    CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA
    GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA
    CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT
    TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT
    CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA
    CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG
    ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA
    GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA
    GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA
    AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT
    CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG
    TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG
    CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT
    GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA
    GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG
    GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA
    AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG
    CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG
    CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT
    GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT
    GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC
    AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA
    CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA
    CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT
    TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA
    ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA
    GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG
    AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA
    AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA
    GCTACAACAC AAGTAAGTGA AGTAACTGCT CAGCCTCCCC CTGAAGAGGA GGAAGATCAA
    AATGAAGATC AAGATGTTCC AGATCTTACT AATTTTACAG TTGAAGAATT GAAACAACGT
    TATGACAGTG TTATAAATCG ACTGTACACT GGTATTCAGT GTTACTCTTG TGGAATGAGG
    TTTACAACAT CACAGACAGA TGTTTATGCA GATCATTTGG ACTGGCATTA TCGGCAAAAT
    AGAACTGAAA AGGATGTTAG CCGAAAAGTC ACTCATAGAC GTTGGTACTA CAGTTTAACA
    GACTGGATAG AATTTGAGGA GATAGCTGAT CTGGAAGAAC GGGCAAAGAG CCAGTTTTTT
    GAAAAGGTGC ATGAAGAAGT TGTGCTCAAA ACTCAAGAGG CTGCTAAAGA AAAAGAGTTC
    CAAAGTGTAC CTGCTGGACC AGCTGGAGCA GTTGAGAGTT GTGAAATCTG TCAAGAACAA
    TTTGAACAAT ACTGGGATGA AGAAGAGGAG GAATGGCATT TGAAAAATGC TATTAGAGTA
    GATGGAAAGA TTTATCATCC ATCATGTTAT GAAGATTATC AAAATACATC TTCATTTGAT
    TGTACACCAT CTCCCAGCAA GACACCAGTT GAAAACCCCT TGAATATTAT GTTGAACATT
    GTCAAAAACG AATTGCAGGA ACCCTGTGAC AGTCCCAAAG TTAAGGAAGA ACGAATTGAT
    ACACCACCAG CTTGTACAGA GGAAAGCATA GCAACACCCT CTGAAATTAA AACAGAAAAT
    GACACAGTCG AGTCAGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45368
    Clone ID Related Accession (Same CDS sequence) NM_001346414.2 , NM_001346414.1
    Accession Version NM_001346414.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-22
    Organism Homo sapiens(human)
    Product pre-mRNA cleavage complex 2 protein Pcf11 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000873.5. On Jun 2, 2019 this sequence version replaced NM_001346414.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.23977.1, SRR1803616.226501.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT
    AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT
    CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG
    GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG
    ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG
    AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT
    CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA
    CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT
    CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA
    GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA
    CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT
    TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT
    CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA
    CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG
    ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA
    GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA
    GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA
    AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT
    CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG
    TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG
    CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT
    GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA
    GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG
    GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA
    AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG
    CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG
    CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT
    GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT
    GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC
    AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA
    CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA
    CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT
    TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA
    ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA
    GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG
    AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA
    AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA
    GCTACAACAC TAAGTGAAGT AACTGCTCAG CCTCCCCCTG AAGAGGAGGA AGATCAAAAT
    GAAGATCAAG ATGTTCCAGA TCTTACTAAT TTTACAGTTG AAGAATTGAA ACAACGTTAT
    GACAGTGTTA TAAATCGACT GTACACTGGT ATTCAGTGTT ACTCTTGTGG AATGAGGTTT
    ACAACATCAC AGACAGATGT TTATGCAGAT CATTTGGACT GGCATTATCG GCAAAATAGA
    ACTGAAAAGG ATGTTAGCCG AAAAGTCACT CATAGACGTT GGTACTACAG TTTAACAGAC
    TGGATAGAAT TTGAGGAGAT AGCTGATCTG GAAGAACGGG CAAAGAGCCA GTTTTTTGAA
    AAGGTGCATG AAGAAGTTGT GCTCAAAACT CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA
    AGTGTACCTG CTGGACCAGC TGGAGCAGTT GAGAGTTGTG AAATCTGTCA AGAACAATTT
    GAACAATACT GGGATGAAGA AGAGGAGGAA TGGCATTTGA AAAATGCTAT TAGAGTAGAT
    GGAAAGATTT ATCATCCATC ATGTTATGAA GATTATCAAA ATACATCTTC ATTTGATTGT
    ACACCATCTC CCAGCAAGAC ACCAGTTGAA AACCCCTTGA ATATTATGTT GAACATTGTC
    AAAAACGAAT TGCAGGAACC CTGTGACAGT CCCAAAGTTA AGGAAGAACG AATTGATACA
    CCACCAGCTT GTACAGAGGA AAGCATAGCA ACACCCTCTG AAATTAAAAC AGAAAATGAC
    ACAGTCGAGT CAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333343.1
    CDS310..5367
    Translation

    Target ORF information:

    RefSeq Version NM_001346414.2
    Organism Homo sapiens(human)
    Definition Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346414.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT
    AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT
    CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG
    GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG
    ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG
    AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT
    CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA
    CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT
    CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA
    GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA
    CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT
    TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT
    CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA
    CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG
    ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA
    GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA
    GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA
    AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT
    CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG
    TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG
    CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT
    GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA
    GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG
    GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA
    AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG
    CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG
    CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT
    GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT
    GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC
    AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA
    CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA
    CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT
    TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA
    ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA
    GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG
    AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA
    AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA
    GCTACAACAC TAAGTGAAGT AACTGCTCAG CCTCCCCCTG AAGAGGAGGA AGATCAAAAT
    GAAGATCAAG ATGTTCCAGA TCTTACTAAT TTTACAGTTG AAGAATTGAA ACAACGTTAT
    GACAGTGTTA TAAATCGACT GTACACTGGT ATTCAGTGTT ACTCTTGTGG AATGAGGTTT
    ACAACATCAC AGACAGATGT TTATGCAGAT CATTTGGACT GGCATTATCG GCAAAATAGA
    ACTGAAAAGG ATGTTAGCCG AAAAGTCACT CATAGACGTT GGTACTACAG TTTAACAGAC
    TGGATAGAAT TTGAGGAGAT AGCTGATCTG GAAGAACGGG CAAAGAGCCA GTTTTTTGAA
    AAGGTGCATG AAGAAGTTGT GCTCAAAACT CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA
    AGTGTACCTG CTGGACCAGC TGGAGCAGTT GAGAGTTGTG AAATCTGTCA AGAACAATTT
    GAACAATACT GGGATGAAGA AGAGGAGGAA TGGCATTTGA AAAATGCTAT TAGAGTAGAT
    GGAAAGATTT ATCATCCATC ATGTTATGAA GATTATCAAA ATACATCTTC ATTTGATTGT
    ACACCATCTC CCAGCAAGAC ACCAGTTGAA AACCCCTTGA ATATTATGTT GAACATTGTC
    AAAAACGAAT TGCAGGAACC CTGTGACAGT CCCAAAGTTA AGGAAGAACG AATTGATACA
    CCACCAGCTT GTACAGAGGA AAGCATAGCA ACACCCTCTG AAATTAAAAC AGAAAATGAC
    ACAGTCGAGT CAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45368
    Clone ID Related Accession (Same CDS sequence) NM_001346414.2 , NM_001346414.1
    Accession Version NM_001346414.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-22
    Organism Homo sapiens(human)
    Product pre-mRNA cleavage complex 2 protein Pcf11 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000873.5. On Oct 9, 2016 this sequence version replaced XM_005274049.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.23977.1, SRR1803616.226501.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT
    AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT
    CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG
    GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG
    ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG
    AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT
    CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA
    CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT
    CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA
    GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA
    CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT
    TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT
    CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA
    CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG
    ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA
    GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA
    GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA
    AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT
    CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG
    TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG
    CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT
    GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA
    GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG
    GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA
    AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG
    CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG
    CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT
    GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT
    GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC
    AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA
    CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA
    CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT
    TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA
    ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA
    GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG
    AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA
    AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA
    GCTACAACAC TAAGTGAAGT AACTGCTCAG CCTCCCCCTG AAGAGGAGGA AGATCAAAAT
    GAAGATCAAG ATGTTCCAGA TCTTACTAAT TTTACAGTTG AAGAATTGAA ACAACGTTAT
    GACAGTGTTA TAAATCGACT GTACACTGGT ATTCAGTGTT ACTCTTGTGG AATGAGGTTT
    ACAACATCAC AGACAGATGT TTATGCAGAT CATTTGGACT GGCATTATCG GCAAAATAGA
    ACTGAAAAGG ATGTTAGCCG AAAAGTCACT CATAGACGTT GGTACTACAG TTTAACAGAC
    TGGATAGAAT TTGAGGAGAT AGCTGATCTG GAAGAACGGG CAAAGAGCCA GTTTTTTGAA
    AAGGTGCATG AAGAAGTTGT GCTCAAAACT CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA
    AGTGTACCTG CTGGACCAGC TGGAGCAGTT GAGAGTTGTG AAATCTGTCA AGAACAATTT
    GAACAATACT GGGATGAAGA AGAGGAGGAA TGGCATTTGA AAAATGCTAT TAGAGTAGAT
    GGAAAGATTT ATCATCCATC ATGTTATGAA GATTATCAAA ATACATCTTC ATTTGATTGT
    ACACCATCTC CCAGCAAGAC ACCAGTTGAA AACCCCTTGA ATATTATGTT GAACATTGTC
    AAAAACGAAT TGCAGGAACC CTGTGACAGT CCCAAAGTTA AGGAAGAACG AATTGATACA
    CCACCAGCTT GTACAGAGGA AAGCATAGCA ACACCCTCTG AAATTAAAAC AGAAAATGAC
    ACAGTCGAGT CAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001333343.1
    CDS346..5403
    Translation

    Target ORF information:

    RefSeq Version NM_001346414.1
    Organism Homo sapiens(human)
    Definition Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001346414.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
    GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG
    ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC
    CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT
    GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT
    ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT
    ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA
    TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT
    GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC
    AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA
    GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG
    AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT
    CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG
    ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT
    CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC
    AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC
    ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA
    TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA
    GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC
    ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA
    GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA
    GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC
    AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA
    ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA
    AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG
    CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG
    ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA
    GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG
    ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA
    CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT
    TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG
    CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA
    CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC
    AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT
    GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT
    AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT
    CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG
    GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG
    ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG
    AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT
    CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA
    CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT
    CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA
    GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA
    CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT
    TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT
    CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA
    CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG
    ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA
    GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA
    GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA
    AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT
    CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG
    TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG
    CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT
    GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA
    GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG
    GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA
    AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG
    CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG
    CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT
    GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT
    GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC
    AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA
    CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA
    CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT
    TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA
    ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA
    GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG
    AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA
    AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA
    GCTACAACAC TAAGTGAAGT AACTGCTCAG CCTCCCCCTG AAGAGGAGGA AGATCAAAAT
    GAAGATCAAG ATGTTCCAGA TCTTACTAAT TTTACAGTTG AAGAATTGAA ACAACGTTAT
    GACAGTGTTA TAAATCGACT GTACACTGGT ATTCAGTGTT ACTCTTGTGG AATGAGGTTT
    ACAACATCAC AGACAGATGT TTATGCAGAT CATTTGGACT GGCATTATCG GCAAAATAGA
    ACTGAAAAGG ATGTTAGCCG AAAAGTCACT CATAGACGTT GGTACTACAG TTTAACAGAC
    TGGATAGAAT TTGAGGAGAT AGCTGATCTG GAAGAACGGG CAAAGAGCCA GTTTTTTGAA
    AAGGTGCATG AAGAAGTTGT GCTCAAAACT CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA
    AGTGTACCTG CTGGACCAGC TGGAGCAGTT GAGAGTTGTG AAATCTGTCA AGAACAATTT
    GAACAATACT GGGATGAAGA AGAGGAGGAA TGGCATTTGA AAAATGCTAT TAGAGTAGAT
    GGAAAGATTT ATCATCCATC ATGTTATGAA GATTATCAAA ATACATCTTC ATTTGATTGT
    ACACCATCTC CCAGCAAGAC ACCAGTTGAA AACCCCTTGA ATATTATGTT GAACATTGTC
    AAAAACGAAT TGCAGGAACC CTGTGACAGT CCCAAAGTTA AGGAAGAACG AATTGATACA
    CCACCAGCTT GTACAGAGGA AAGCATAGCA ACACCCTCTG AAATTAAAAC AGAAAATGAC
    ACAGTCGAGT CAGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.