NOL7 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOL7
Entrez Gene ID	51406
Full Name	nucleolar protein 7
Synonyms	C6orf90,PQBP3,RARG-1,dJ223E5.2
General protein information	Preferred Names nucleolar protein 7 Names nucleolar protein 7 nucleolar protein 7, 27kDa nucleolar protein of 27 kDa polyglutamine binding protein 3 retinoic acid repressible protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p23
Summary	The protein encoded by this gene localizes to the nucleolus, where it maintains nucleolar structure and cell growth rates. The encoded protein also functions as a tumor suppressor and regulator of angiogenesis. The RB tumor suppressor gene recruits transcription factors to this gene and positively regulates its expression. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2015].
Disorder MIM:	611533

mRNA and Protein(s)

mRNA	Protein	Name
NM_016167.3	NP_057251.2	nucleolar protein 7
NM_016167.5	NP_057251.2	nucleolar protein 7
XM_005249173.3	XP_005249230.1	nucleolar protein 7 isoform X1
NM_016167.4	NP_057251.2	nucleolar protein 7
NM_001317724.1	NP_001304653.1	nucleolar protein 7

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NOL7	XP_518245.3
Canis lupus familiaris (dog)	NOL7	XP_535892.3
	NOL7	XP_001092572.1
Mus musculus (house mouse)	Nol7	NP_076043.2
Danio rerio (zebrafish)	nol7	XP_687281.4
Xenopus tropicalis (tropical clawed frog)	LOC100489073	NP_001263645.1
Homo sapiens (human)	NOL7	NP_057251.2
Rattus norvegicus (Norway rat)	Nol7	XP_006253911.1
Bos taurus (cattle)	NOL7	NP_001029556.1
Gallus gallus (chicken)	NOL7	XP_004939690.1

Gene Ontology
Bibliography

Related articles in PubMed

The novel tumor suppressor NOL7 post-transcriptionally regulates thrombospondin-1 expression.
Do?i CL, Zhou G, Lingen MW
Oncogene32(37)4377-86(2013 Sep)

The RB tumor suppressor positively regulates transcription of the anti-angiogenic protein NOL7.
Mankame TP, Lingen MW
Neoplasia (New York, N.Y.)14(12)1213-22(2012 Dec)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Characterization of NOL7 gene point mutations, promoter methylation, and protein expression in cervical cancer.
Do?i CL, Mankame TP, Langerman A, Ostler KR, Kanteti R, Best T, Onel K, Godley LA, Salgia R, Lingen MW
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists31(1)15-24(2012 Jan)

The dynamics of the alternatively spliced NOL7 gene products and role in nucleolar architecture.
Kinor N, Shav-Tal Y
Nucleus (Austin, Tex.)2(3)229-45(2011 May-Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NOL7 significantly alters TSP-1 expression and may be a master regulator that coordinates the post-transcriptional expression of key signaling factors critical for the regulation of the angiogenic phenotype.
Title: The novel tumor suppressor NOL7 post-transcriptionally regulates thrombospondin-1 expression.

NOL7 is the legitimate tumor suppressor gene located on 6p23.
Title: Characterization of NOL7 gene point mutations, promoter methylation, and protein expression in cervical cancer.

Three alternatively spliced forms of the human NOL7 gene coding for relatively small proteins were identified
Title: The dynamics of the alternatively spliced NOL7 gene products and role in nucleolar architecture.

determined that the essential elements required for the optimal promoter activity of NOL7 were 560 bp upstream of its translation start site.
Title: Identification and characterization of the human NOL7 gene promoter.

The following NOL7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOL7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15793	NM_001317724.1 Latest version!	Homo sapiens nucleolar protein 7 (NOL7), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu15793	NM_016167.5 Latest version!	Homo sapiens nucleolar protein 7 (NOL7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu15793	XM_005249173.3 Latest version!	Homo sapiens nucleolar protein 7, 27kDa (NOL7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu15793	NM_016167.3	Homo sapiens nucleolar protein 7, 27kDa (NOL7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu15793	NM_016167.4	Homo sapiens nucleolar protein 7 (NOL7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOL7 ( NM_016167.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057251.2 Homo sapiens nucleolar protein 7, 27kDa (NOL7), mRNA.

Price & Availability↑

CloneID	OHu15793
Clone ID Related Accession (Same CDS sequence)	NM_016167.3 , NM_016167.5 , XM_005249173.3 , NM_016167.4 , NM_001317724.1
Accession Version	NM_016167.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	nucleolar protein 7
Comment

ATGGTGCAGC TCCGACCGCG AGCGTCTCGC GCCCCGGCGT CGGCGGAGGC GATGGTGGAC 
GAGGGCCAGC TGGCCTCGGA GGAGGAGGAG GCGGAGCACG GGCTGTTGCT CGGGCAGCCC 
AGCAGCGGCG CGGCCGCCGA GCCCCTGGAG GAAGACGAGG AAGGGGACGA TGAGTTTGAC 
GATGAGGCCC CGGAGGAGCT GACTTTCGCC AGCGCCCAGG CGGAAGCGAG AGAAGAGGAG 
CGGCGAGTGC GGGAGACCGT GCGCAGGGAT AAAACGCTCC TGAAGGAGAA GAGGAAGCGA 
CGCGAGGAGC TGTTCATCGA ACAGAAGAAA AGAAAACTCC TTCCAGACAC TATTTTGGAG 
AAGTTAACCA CAGCTTCACA GACTAACATC AAGAAATCGC CAGGAAAGGT GAAAGAAGTT 
AATTTGCAAA AGAAAAATGA AGACTGTGAA AAAGGAAATG ACTCCAAGAA AGTTAAAGTA 
CAAAAAGTAC AGTCTGTCAG CCAGAATAAA AGCTACTTGG CCGTAAGGCT AAAAGACCAA 
GATCTGAGAG ATTCAAGGCA ACAAGCAGCA CAAGCCTTCA TACATAATTC ATTATATGGG 
CCAGGAACCA ACAGGACTAC TGTAAATAAG TTCCTGTCTC TTGCCAACAA GAGGTTACCA 
GTGAAAAGAG CTGCTGTCCA GTTTTTGAAT AATGCTTGGG GAATCCAAAA AAAACAAAAT 
GCCAAGAGGT TTAAAAGACG GTGGATGGTC AGAAAGATGA AAACTAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057251.2
Translation	MVQLRPRASRAPASAEAMVDEGQLASEEEEAEHGLLLGQPSSGAAAEPLEEDEEGDDEFDDEAPEELTFASAQAEAREEERRVRETVRRDKTLLKEKRKRREELFIEQKKRKLLPDTILEKLTTASQTNIKKSPGKVKEVNLQKKNEDCEKGNDSKKVKVQKVQSVSQNKSYLAVRLKDQDLRDSRQQAAQAFIHNSLYGPGTNRTTVNKFLSLANKRLPVKRAAVQFLNNAWGIQKKQNAKRFKRRWMVRKMKTKK

Target ORF information:

RefSeq Version	NM_016167.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens nucleolar protein 7, 27kDa (NOL7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016167.3

ORF Insert Sequence:

ATGGTGCAGC TCCGACCGCG AGCGTCTCGC GCCCCGGCGT CGGCGGAGGC GATGGTGGAC 
GAGGGCCAGC TGGCCTCGGA GGAGGAGGAG GCGGAGCACG GGCTGTTGCT CGGGCAGCCC 
AGCAGCGGCG CGGCCGCCGA GCCCCTGGAG GAAGACGAGG AAGGGGACGA TGAGTTTGAC 
GATGAGGCCC CGGAGGAGCT GACTTTCGCC AGCGCCCAGG CGGAAGCGAG AGAAGAGGAG 
CGGCGAGTGC GGGAGACCGT GCGCAGGGAT AAAACGCTCC TGAAGGAGAA GAGGAAGCGA 
CGCGAGGAGC TGTTCATCGA ACAGAAGAAA AGAAAACTCC TTCCAGACAC TATTTTGGAG 
AAGTTAACCA CAGCTTCACA GACTAACATC AAGAAATCGC CAGGAAAGGT GAAAGAAGTT 
AATTTGCAAA AGAAAAATGA AGACTGTGAA AAAGGAAATG ACTCCAAGAA AGTTAAAGTA 
CAAAAAGTAC AGTCTGTCAG CCAGAATAAA AGCTACTTGG CCGTAAGGCT AAAAGACCAA 
GATCTGAGAG ATTCAAGGCA ACAAGCAGCA CAAGCCTTCA TACATAATTC ATTATATGGG 
CCAGGAACCA ACAGGACTAC TGTAAATAAG TTCCTGTCTC TTGCCAACAA GAGGTTACCA 
GTGAAAAGAG CTGCTGTCCA GTTTTTGAAT AATGCTTGGG GAATCCAAAA AAAACAAAAT 
GCCAAGAGGT TTAAAAGACG GTGGATGGTC AGAAAGATGA AAACTAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOL7 ( NM_016167.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057251.2 Homo sapiens nucleolar protein 7 (NOL7), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15793
Clone ID Related Accession (Same CDS sequence)	NM_016167.3 , NM_016167.5 , XM_005249173.3 , NM_016167.4 , NM_001317724.1
Accession Version	NM_016167.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	nucleolar protein 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF172066.3, CX760811.1 and BM510944.1. On Nov 23, 2018 this sequence version replaced NM_016167.4. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.97670.1, SRR1660807.223088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000451315.7/ ENSP00000405674.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGCAGC TCCGACCGCG AGCGTCTCGC GCCCCGGCGT CGGCGGAGGC GATGGTGGAC 
GAGGGCCAGC TGGCCTCGGA GGAGGAGGAG GCGGAGCACG GGCTGTTGCT CGGGCAGCCC 
AGCAGCGGCG CGGCCGCCGA GCCCCTGGAG GAAGACGAGG AAGGGGACGA TGAGTTTGAC 
GATGAGGCCC CGGAGGAGCT GACTTTCGCC AGCGCCCAGG CGGAAGCGAG AGAAGAGGAG 
CGGCGAGTGC GGGAGACCGT GCGCAGGGAT AAAACGCTCC TGAAGGAGAA GAGGAAGCGA 
CGCGAGGAGC TGTTCATCGA ACAGAAGAAA AGAAAACTCC TTCCAGACAC TATTTTGGAG 
AAGTTAACCA CAGCTTCACA GACTAACATC AAGAAATCGC CAGGAAAGGT GAAAGAAGTT 
AATTTGCAAA AGAAAAATGA AGACTGTGAA AAAGGAAATG ACTCCAAGAA AGTTAAAGTA 
CAAAAAGTAC AGTCTGTCAG CCAGAATAAA AGCTACTTGG CCGTAAGGCT AAAAGACCAA 
GATCTGAGAG ATTCAAGGCA ACAAGCAGCA CAAGCCTTCA TACATAATTC ATTATATGGG 
CCAGGAACCA ACAGGACTAC TGTAAATAAG TTCCTGTCTC TTGCCAACAA GAGGTTACCA 
GTGAAAAGAG CTGCTGTCCA GTTTTTGAAT AATGCTTGGG GAATCCAAAA AAAACAAAAT 
GCCAAGAGGT TTAAAAGACG GTGGATGGTC AGAAAGATGA AAACTAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057251.2
CDS	25..798
Translation	MVQLRPRASRAPASAEAMVDEGQLASEEEEAEHGLLLGQPSSGAAAEPLEEDEEGDDEFDDEAPEELTFASAQAEAREEERRVRETVRRDKTLLKEKRKRREELFIEQKKRKLLPDTILEKLTTASQTNIKKSPGKVKEVNLQKKNEDCEKGNDSKKVKVQKVQSVSQNKSYLAVRLKDQDLRDSRQQAAQAFIHNSLYGPGTNRTTVNKFLSLANKRLPVKRAAVQFLNNAWGIQKKQNAKRFKRRWMVRKMKTKK

Target ORF information:

RefSeq Version	NM_016167.5
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar protein 7 (NOL7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016167.5

ORF Insert Sequence:

ATGGTGCAGC TCCGACCGCG AGCGTCTCGC GCCCCGGCGT CGGCGGAGGC GATGGTGGAC 
GAGGGCCAGC TGGCCTCGGA GGAGGAGGAG GCGGAGCACG GGCTGTTGCT CGGGCAGCCC 
AGCAGCGGCG CGGCCGCCGA GCCCCTGGAG GAAGACGAGG AAGGGGACGA TGAGTTTGAC 
GATGAGGCCC CGGAGGAGCT GACTTTCGCC AGCGCCCAGG CGGAAGCGAG AGAAGAGGAG 
CGGCGAGTGC GGGAGACCGT GCGCAGGGAT AAAACGCTCC TGAAGGAGAA GAGGAAGCGA 
CGCGAGGAGC TGTTCATCGA ACAGAAGAAA AGAAAACTCC TTCCAGACAC TATTTTGGAG 
AAGTTAACCA CAGCTTCACA GACTAACATC AAGAAATCGC CAGGAAAGGT GAAAGAAGTT 
AATTTGCAAA AGAAAAATGA AGACTGTGAA AAAGGAAATG ACTCCAAGAA AGTTAAAGTA 
CAAAAAGTAC AGTCTGTCAG CCAGAATAAA AGCTACTTGG CCGTAAGGCT AAAAGACCAA 
GATCTGAGAG ATTCAAGGCA ACAAGCAGCA CAAGCCTTCA TACATAATTC ATTATATGGG 
CCAGGAACCA ACAGGACTAC TGTAAATAAG TTCCTGTCTC TTGCCAACAA GAGGTTACCA 
GTGAAAAGAG CTGCTGTCCA GTTTTTGAAT AATGCTTGGG GAATCCAAAA AAAACAAAAT 
GCCAAGAGGT TTAAAAGACG GTGGATGGTC AGAAAGATGA AAACTAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOL7 ( NM_016167.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057251.2 Homo sapiens nucleolar protein 7 (NOL7), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15793
Clone ID Related Accession (Same CDS sequence)	NM_016167.3 , NM_016167.5 , XM_005249173.3 , NM_016167.4 , NM_001317724.1
Accession Version	NM_016167.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	nucleolar protein 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF172066.3, CX760811.1 and BM510944.1. On Dec 4, 2015 this sequence version replaced NM_016167.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF172066.3, SRR1660807.223088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTGCAGC TCCGACCGCG AGCGTCTCGC GCCCCGGCGT CGGCGGAGGC GATGGTGGAC 
GAGGGCCAGC TGGCCTCGGA GGAGGAGGAG GCGGAGCACG GGCTGTTGCT CGGGCAGCCC 
AGCAGCGGCG CGGCCGCCGA GCCCCTGGAG GAAGACGAGG AAGGGGACGA TGAGTTTGAC 
GATGAGGCCC CGGAGGAGCT GACTTTCGCC AGCGCCCAGG CGGAAGCGAG AGAAGAGGAG 
CGGCGAGTGC GGGAGACCGT GCGCAGGGAT AAAACGCTCC TGAAGGAGAA GAGGAAGCGA 
CGCGAGGAGC TGTTCATCGA ACAGAAGAAA AGAAAACTCC TTCCAGACAC TATTTTGGAG 
AAGTTAACCA CAGCTTCACA GACTAACATC AAGAAATCGC CAGGAAAGGT GAAAGAAGTT 
AATTTGCAAA AGAAAAATGA AGACTGTGAA AAAGGAAATG ACTCCAAGAA AGTTAAAGTA 
CAAAAAGTAC AGTCTGTCAG CCAGAATAAA AGCTACTTGG CCGTAAGGCT AAAAGACCAA 
GATCTGAGAG ATTCAAGGCA ACAAGCAGCA CAAGCCTTCA TACATAATTC ATTATATGGG 
CCAGGAACCA ACAGGACTAC TGTAAATAAG TTCCTGTCTC TTGCCAACAA GAGGTTACCA 
GTGAAAAGAG CTGCTGTCCA GTTTTTGAAT AATGCTTGGG GAATCCAAAA AAAACAAAAT 
GCCAAGAGGT TTAAAAGACG GTGGATGGTC AGAAAGATGA AAACTAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057251.2
CDS	33..806
Translation	MVQLRPRASRAPASAEAMVDEGQLASEEEEAEHGLLLGQPSSGAAAEPLEEDEEGDDEFDDEAPEELTFASAQAEAREEERRVRETVRRDKTLLKEKRKRREELFIEQKKRKLLPDTILEKLTTASQTNIKKSPGKVKEVNLQKKNEDCEKGNDSKKVKVQKVQSVSQNKSYLAVRLKDQDLRDSRQQAAQAFIHNSLYGPGTNRTTVNKFLSLANKRLPVKRAAVQFLNNAWGIQKKQNAKRFKRRWMVRKMKTKK

Target ORF information:

RefSeq Version	NM_016167.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar protein 7 (NOL7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016167.4

ORF Insert Sequence:

ATGGTGCAGC TCCGACCGCG AGCGTCTCGC GCCCCGGCGT CGGCGGAGGC GATGGTGGAC 
GAGGGCCAGC TGGCCTCGGA GGAGGAGGAG GCGGAGCACG GGCTGTTGCT CGGGCAGCCC 
AGCAGCGGCG CGGCCGCCGA GCCCCTGGAG GAAGACGAGG AAGGGGACGA TGAGTTTGAC 
GATGAGGCCC CGGAGGAGCT GACTTTCGCC AGCGCCCAGG CGGAAGCGAG AGAAGAGGAG 
CGGCGAGTGC GGGAGACCGT GCGCAGGGAT AAAACGCTCC TGAAGGAGAA GAGGAAGCGA 
CGCGAGGAGC TGTTCATCGA ACAGAAGAAA AGAAAACTCC TTCCAGACAC TATTTTGGAG 
AAGTTAACCA CAGCTTCACA GACTAACATC AAGAAATCGC CAGGAAAGGT GAAAGAAGTT 
AATTTGCAAA AGAAAAATGA AGACTGTGAA AAAGGAAATG ACTCCAAGAA AGTTAAAGTA 
CAAAAAGTAC AGTCTGTCAG CCAGAATAAA AGCTACTTGG CCGTAAGGCT AAAAGACCAA 
GATCTGAGAG ATTCAAGGCA ACAAGCAGCA CAAGCCTTCA TACATAATTC ATTATATGGG 
CCAGGAACCA ACAGGACTAC TGTAAATAAG TTCCTGTCTC TTGCCAACAA GAGGTTACCA 
GTGAAAAGAG CTGCTGTCCA GTTTTTGAAT AATGCTTGGG GAATCCAAAA AAAACAAAAT 
GCCAAGAGGT TTAAAAGACG GTGGATGGTC AGAAAGATGA AAACTAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOL7 ( NM_001317724.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304653.1 Homo sapiens nucleolar protein 7 (NOL7), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15793
Clone ID Related Accession (Same CDS sequence)	NM_016167.3 , NM_016167.5 , XM_005249173.3 , NM_016167.4 , NM_001317724.1
Accession Version	NM_001317724.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	nucleolar protein 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF172066.3 and BU071935.1. On Dec 4, 2015 this sequence version replaced XM_005249173.3. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU071935.1, SRR1803616.312626.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 5' end.

ATGGTGCAGC TCCGACCGCG AGCGTCTCGC GCCCCGGCGT CGGCGGAGGC GATGGTGGAC 
GAGGGCCAGC TGGCCTCGGA GGAGGAGGAG GCGGAGCACG GGCTGTTGCT CGGGCAGCCC 
AGCAGCGGCG CGGCCGCCGA GCCCCTGGAG GAAGACGAGG AAGGGGACGA TGAGTTTGAC 
GATGAGGCCC CGGAGGAGCT GACTTTCGCC AGCGCCCAGG CGGAAGCGAG AGAAGAGGAG 
CGGCGAGTGC GGGAGACCGT GCGCAGGGAT AAAACGCTCC TGAAGGAGAA GAGGAAGCGA 
CGCGAGGAGC TGTTCATCGA ACAGAAGAAA AGAAAACTCC TTCCAGACAC TATTTTGGAG 
AAGTTAACCA CAGCTTCACA GACTAACATC AAGAAATCGC CAGGAAAGGT GAAAGAAGTT 
AATTTGCAAA AGAAAAATGA AGACTGTGAA AAAGGAAATG ACTCCAAGAA AGTTAAAGTA 
CAAAAAGTAC AGTCTGTCAG CCAGAATAAA AGCTACTTGG CCGTAAGGCT AAAAGACCAA 
GATCTGAGAG ATTCAAGGCA ACAAGCAGCA CAAGCCTTCA TACATAATTC ATTATATGGG 
CCAGGAACCA ACAGGACTAC TGTAAATAAG TTCCTGTCTC TTGCCAACAA GAGGTTACCA 
GTGAAAAGAG CTGCTGTCCA GTTTTTGAAT AATGCTTGGG GAATCCAAAA AAAACAAAAT 
GCCAAGAGGT TTAAAAGACG GTGGATGGTC AGAAAGATGA AAACTAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304653.1
CDS	33..806
Translation	MVQLRPRASRAPASAEAMVDEGQLASEEEEAEHGLLLGQPSSGAAAEPLEEDEEGDDEFDDEAPEELTFASAQAEAREEERRVRETVRRDKTLLKEKRKRREELFIEQKKRKLLPDTILEKLTTASQTNIKKSPGKVKEVNLQKKNEDCEKGNDSKKVKVQKVQSVSQNKSYLAVRLKDQDLRDSRQQAAQAFIHNSLYGPGTNRTTVNKFLSLANKRLPVKRAAVQFLNNAWGIQKKQNAKRFKRRWMVRKMKTKK

Target ORF information:

RefSeq Version	NM_001317724.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nucleolar protein 7 (NOL7), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317724.1

ORF Insert Sequence:

ATGGTGCAGC TCCGACCGCG AGCGTCTCGC GCCCCGGCGT CGGCGGAGGC GATGGTGGAC 
GAGGGCCAGC TGGCCTCGGA GGAGGAGGAG GCGGAGCACG GGCTGTTGCT CGGGCAGCCC 
AGCAGCGGCG CGGCCGCCGA GCCCCTGGAG GAAGACGAGG AAGGGGACGA TGAGTTTGAC 
GATGAGGCCC CGGAGGAGCT GACTTTCGCC AGCGCCCAGG CGGAAGCGAG AGAAGAGGAG 
CGGCGAGTGC GGGAGACCGT GCGCAGGGAT AAAACGCTCC TGAAGGAGAA GAGGAAGCGA 
CGCGAGGAGC TGTTCATCGA ACAGAAGAAA AGAAAACTCC TTCCAGACAC TATTTTGGAG 
AAGTTAACCA CAGCTTCACA GACTAACATC AAGAAATCGC CAGGAAAGGT GAAAGAAGTT 
AATTTGCAAA AGAAAAATGA AGACTGTGAA AAAGGAAATG ACTCCAAGAA AGTTAAAGTA 
CAAAAAGTAC AGTCTGTCAG CCAGAATAAA AGCTACTTGG CCGTAAGGCT AAAAGACCAA 
GATCTGAGAG ATTCAAGGCA ACAAGCAGCA CAAGCCTTCA TACATAATTC ATTATATGGG 
CCAGGAACCA ACAGGACTAC TGTAAATAAG TTCCTGTCTC TTGCCAACAA GAGGTTACCA 
GTGAAAAGAG CTGCTGTCCA GTTTTTGAAT AATGCTTGGG GAATCCAAAA AAAACAAAAT 
GCCAAGAGGT TTAAAAGACG GTGGATGGTC AGAAAGATGA AAACTAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOL7 ( XM_005249173.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249230.1 Homo sapiens nucleolar protein 7, 27kDa (NOL7), transcript variant X1, mRNA.

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CloneID	OHu15793
Clone ID Related Accession (Same CDS sequence)	NM_016167.3 , NM_016167.5 , XM_005249173.3 , NM_016167.4 , NM_001317724.1
Accession Version	XM_005249173.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	nucleolar protein 7 isoform X1
Comment

ATGGTGCAGC TCCGACCGCG AGCGTCTCGC GCCCCGGCGT CGGCGGAGGC GATGGTGGAC 
GAGGGCCAGC TGGCCTCGGA GGAGGAGGAG GCGGAGCACG GGCTGTTGCT CGGGCAGCCC 
AGCAGCGGCG CGGCCGCCGA GCCCCTGGAG GAAGACGAGG AAGGGGACGA TGAGTTTGAC 
GATGAGGCCC CGGAGGAGCT GACTTTCGCC AGCGCCCAGG CGGAAGCGAG AGAAGAGGAG 
CGGCGAGTGC GGGAGACCGT GCGCAGGGAT AAAACGCTCC TGAAGGAGAA GAGGAAGCGA 
CGCGAGGAGC TGTTCATCGA ACAGAAGAAA AGAAAACTCC TTCCAGACAC TATTTTGGAG 
AAGTTAACCA CAGCTTCACA GACTAACATC AAGAAATCGC CAGGAAAGGT GAAAGAAGTT 
AATTTGCAAA AGAAAAATGA AGACTGTGAA AAAGGAAATG ACTCCAAGAA AGTTAAAGTA 
CAAAAAGTAC AGTCTGTCAG CCAGAATAAA AGCTACTTGG CCGTAAGGCT AAAAGACCAA 
GATCTGAGAG ATTCAAGGCA ACAAGCAGCA CAAGCCTTCA TACATAATTC ATTATATGGG 
CCAGGAACCA ACAGGACTAC TGTAAATAAG TTCCTGTCTC TTGCCAACAA GAGGTTACCA 
GTGAAAAGAG CTGCTGTCCA GTTTTTGAAT AATGCTTGGG GAATCCAAAA AAAACAAAAT 
GCCAAGAGGT TTAAAAGACG GTGGATGGTC AGAAAGATGA AAACTAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249230.1
Translation	MVQLRPRASRAPASAEAMVDEGQLASEEEEAEHGLLLGQPSSGAAAEPLEEDEEGDDEFDDEAPEELTFASAQAEAREEERRVRETVRRDKTLLKEKRKRREELFIEQKKRKLLPDTILEKLTTASQTNIKKSPGKVKEVNLQKKNEDCEKGNDSKKVKVQKVQSVSQNKSYLAVRLKDQDLRDSRQQAAQAFIHNSLYGPGTNRTTVNKFLSLANKRLPVKRAAVQFLNNAWGIQKKQNAKRFKRRWMVRKMKTKK

Target ORF information:

RefSeq Version	XM_005249173.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens nucleolar protein 7, 27kDa (NOL7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249173.3

ORF Insert Sequence:

ATGGTGCAGC TCCGACCGCG AGCGTCTCGC GCCCCGGCGT CGGCGGAGGC GATGGTGGAC 
GAGGGCCAGC TGGCCTCGGA GGAGGAGGAG GCGGAGCACG GGCTGTTGCT CGGGCAGCCC 
AGCAGCGGCG CGGCCGCCGA GCCCCTGGAG GAAGACGAGG AAGGGGACGA TGAGTTTGAC 
GATGAGGCCC CGGAGGAGCT GACTTTCGCC AGCGCCCAGG CGGAAGCGAG AGAAGAGGAG 
CGGCGAGTGC GGGAGACCGT GCGCAGGGAT AAAACGCTCC TGAAGGAGAA GAGGAAGCGA 
CGCGAGGAGC TGTTCATCGA ACAGAAGAAA AGAAAACTCC TTCCAGACAC TATTTTGGAG 
AAGTTAACCA CAGCTTCACA GACTAACATC AAGAAATCGC CAGGAAAGGT GAAAGAAGTT 
AATTTGCAAA AGAAAAATGA AGACTGTGAA AAAGGAAATG ACTCCAAGAA AGTTAAAGTA 
CAAAAAGTAC AGTCTGTCAG CCAGAATAAA AGCTACTTGG CCGTAAGGCT AAAAGACCAA 
GATCTGAGAG ATTCAAGGCA ACAAGCAGCA CAAGCCTTCA TACATAATTC ATTATATGGG 
CCAGGAACCA ACAGGACTAC TGTAAATAAG TTCCTGTCTC TTGCCAACAA GAGGTTACCA 
GTGAAAAGAG CTGCTGTCCA GTTTTTGAAT AATGCTTGGG GAATCCAAAA AAAACAAAAT 
GCCAAGAGGT TTAAAAGACG GTGGATGGTC AGAAAGATGA AAACTAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The novel tumor suppressor NOL7 post-transcriptionally regulates thrombospondin-1 expression.
Oncogene32(37)4377-86(2013 Sep)
Do?i CL,Zhou G,Lingen MW

The RB tumor suppressor positively regulates transcription of the anti-angiogenic protein NOL7.
Neoplasia (New York, N.Y.)14(12)1213-22(2012 Dec)
Mankame TP,Lingen MW

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Characterization of NOL7 gene point mutations, promoter methylation, and protein expression in cervical cancer.
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists31(1)15-24(2012 Jan)
Do?i CL,Mankame TP,Langerman A,Ostler KR,Kanteti R,Best T,Onel K,Godley LA,Salgia R,Lingen MW

The dynamics of the alternatively spliced NOL7 gene products and role in nucleolar architecture.
Nucleus (Austin, Tex.)2(3)229-45(2011 May-Jun)
Kinor N,Shav-Tal Y

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NOL7 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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