ECSIT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ECSIT
Entrez Gene ID	51295
Full Name	ECSIT signalling integrator
Synonyms	SITPEC
General protein information	Preferred Names ECSIT signalling integrator Names evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial ECSIT homolog likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.2
Disorder MIM:	608388

mRNA and Protein(s)

mRNA	Protein	Name
NM_001142464.2	NP_001135936.1	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 2 precursor
NM_016581.4	NP_057665.2	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 1 precursor
NM_001142464.3	NP_001135936.1	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 2 precursor
NM_001142465.2	NP_001135937.1	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 3 precursor
NM_001243204.1	NP_001230133.1	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 4 precursor
NM_001142465.3	NP_001135937.1	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 3 precursor
NM_016581.5	NP_057665.2	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	ECSIT	NP_057665.2
Pan troglodytes (chimpanzee)	ECSIT	XP_512396.2
	LOC714805	XP_001106441.1
Canis lupus familiaris (dog)	ECSIT	XP_542054.2
Mus musculus (house mouse)	Ecsit	NP_001240826.1
Danio rerio (zebrafish)	ecsit	NP_001229867.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005295	XP_309087.4
Caenorhabditis elegans (roundworm)	Y17G9B.5	NP_500631.1
Xenopus tropicalis (tropical clawed frog)	ecsit	NP_001072411.1
Bos taurus (cattle)	ECSIT	NP_001029458.1
Rattus norvegicus (Norway rat)	Ecsit	NP_001006987.1
Gallus gallus (chicken)	ECSIT	XP_003643592.1
Drosophila melanogaster (fruit fly)	ECSIT	NP_649602.2

Gene Ontology
Bibliography

Related articles in PubMed

Recurrent ECSIT mutation encoding V140A triggers hyperinflammation and promotes hemophagocytic syndrome in extranodal NK/T cell lymphoma.
Wen H, Ma H, Cai Q, Lin S, Lei X, He B, Wu S, Wang Z, Gao Y, Liu W, Liu W, Tao Q, Long Z, Yan M, Li D, Kelley KW, Yang Y, Huang H, Liu Q
Nature medicine(2018 Jan)

Hepatitis B virus X protein increases the IL-1?-induced NF-?B activation via interaction with evolutionarily conserved signaling intermediate in Toll pathways (ECSIT).
Chen WN, Liu LL, Jiao BY, Lin WS, Lin XJ, Lin X
Virus research195236-45(2015 Jan)

Ubiquitination of ECSIT is crucial for the activation of p65/p50 NF-?Bs in Toll-like receptor 4 signaling.
Mi Wi S, Park J, Shim JH, Chun E, Lee KY
Molecular biology of the cell26(1)151-60(2015 Jan)

ECSIT bridges RIG-I-like receptors to VISA in signaling events of innate antiviral responses.
Lei CQ, Zhang Y, Li M, Jiang LQ, Zhong B, Kim YH, Shu HB
Journal of innate immunity7(2)153-64(2015)

TAK1-ECSIT-TRAF6 complex plays a key role in the TLR4 signal to activate NF-?B.
Wi SM, Moon G, Kim J, Kim ST, Shim JH, Chun E, Lee KY
The Journal of biological chemistry289(51)35205-14(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors conclude that ECSIT appears to be a novel Hepatitis B virus X protein-interacting signal molecule and their interaction is mechanistically important in IL-1beta induction of NF-kappaB activation.
Title: Hepatitis B virus X protein increases the IL-1?-induced NF-?B activation via interaction with evolutionarily conserved signaling intermediate in Toll pathways (ECSIT).

Ubiquitination of ECSIT might have a role in the regulation of NF-kappaB activity in TLR4 signaling.
Title: Ubiquitination of ECSIT is crucial for the activation of p65/p50 NF-?Bs in Toll-like receptor 4 signaling.

mediates virus-triggered type I IFN induction by bridging RIG-I and MDA5 to the VISA complex
Title: ECSIT bridges RIG-I-like receptors to VISA in signaling events of innate antiviral responses.

Data show that the ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) complex, including MEKK7 (TAK1) and TNF receptor-associated factor 6 (TRAF6), plays a role in Toll-like receptor 4 -mediated signals to activate NF-kappa B.
Title: TAK1-ECSIT-TRAF6 complex plays a key role in the TLR4 signal to activate NF-?B.

TRIM59 interacts with ECSIT as an adaptor protein required for the TLR-mediated transduction pathway.
Title: TRIM59 interacts with ECSIT and negatively regulates NF-?B and IRF-3/7-mediated signal pathways.

The following ECSIT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECSIT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09324	NM_016581.5 Latest version!	Homo sapiens ECSIT signaling integrator (ECSIT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu09453	NM_001142464.3 Latest version!	Homo sapiens ECSIT signaling integrator (ECSIT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu09476	NM_001243204.1 Latest version!	Homo sapiens ECSIT signaling integrator (ECSIT), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu09506	NM_001142465.3 Latest version!	Homo sapiens ECSIT signaling integrator (ECSIT), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
View Old Accession Versions >>
OHu09453	NM_001142464.2	Homo sapiens ECSIT signalling integrator (ECSIT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu09506	NM_001142465.2	Homo sapiens ECSIT signalling integrator (ECSIT), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OHu09324	NM_016581.4	Homo sapiens ECSIT signalling integrator (ECSIT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECSIT ( NM_016581.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057665.2 Homo sapiens ECSIT signalling integrator (ECSIT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09324
Clone ID Related Accession (Same CDS sequence)	NM_016581.4 , NM_016581.5
Accession Version	NM_016581.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE791936.1, BC000193.1 and R46579.1. On Aug 4, 2011 this sequence version replaced NM_016581.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000193.1, SRR1803614.43286.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTCC CTCGCCGGCT CCCTCGGGGC 
CTCCACTGCA GCGCAGCTGC CCATAGCTCT GAACAGTCCC TGGTTCCCAG CCCACCGGAA 
CCCCGGCAGA GGCCCACCAA GGCTCTGGTG CCCTTTGAGG ACCTGTTTGG GCAGGCGCCT 
GGTGGGGAAC GGGACAAGGC GAGCTTCCTG CAGACGGTGC AGAAATTTGC GGAGCACAGC 
GTGCGTAAGC GGGGCCACAT TGACTTCATC TACCTGGCCC TGCGCAAGAT GCGGGAGTAT 
GGTGTCGAGC GGGACCTGGC TGTGTACAAC CAGCTGCTCA ACATCTTCCC CAAGGAGGTC 
TTCCGGCCTC GCAACATCAT CCAGCGCATC TTCGTCCACT ACCCTCGGCA GCAGGAGTGT 
GGGATTGCTG TCCTGGAGCA GATGGAGAAC CACGGTGTGA TGCCCAACAA GGAGACGGAG 
TTCCTGCTGA TTCAGATCTT TGGACGCAAA AGCTACCCCA TGCTCAAGTT GGTGCGCCTG 
AAGCTGTGGT TCCCTCGATT CATGAACGTC AACCCCTTCC CAGTGCCCCG GGACCTGCCC 
CAGGACCCTG TGGAGCTGGC CATGTTTGGC CTGCGGCACA TGGAGCCTGA CCTTAGTGCC 
AGGGTCACCA TCTACCAGGT TCCTTTGCCC AAAGACTCAA CAGGTGCAGC AGATCCCCCC 
CAGCCCCACA TCGTAGGAAT CCAGAGTCCC GATCAGCAGG CCGCCCTGGC CCGCCACAAT 
CCAGCCCGGC CTGTCTTTGT TGAGGGCCCC TTCTCCCTGT GGCTCCGCAA CAAGTGTGTG 
TATTACCACA TCCTCAGAGC TGACTTGCTG CCCCCGGAGG AGAGGGAAGT GGAAGAGACG 
CCGGAGGAGT GGAACCTCTA CTACCCGATG CAGCTGGACC TGGAGTATGT GAGGAGTGGC 
TGGGACAACT ACGAGTTTGA CATCAATGAA GTGGAGGAAG GCCCTGTCTT CGCCATGTGC 
ATGGCGGGTG CTCATGACCA GGCGACGATG GCTAAGTGGA TCCAGGGCCT GCAGGAGACC 
AACCCAACCC TGGCCCAGAT CCCCGTGGTC TTCCGCCTCG CCGGGTCCAC CCGGGAGCTC 
CAGACATCCT CTGCAGGGCT GGAGGAGCCG CCCCTGCCCG AGGACCACCA GGAAGAAGAC 
GACAACCTGC AGCGACAGCA GCAGGGCCAG AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057665.2
CDS	135..1430
Translation	MSWVQATLLARGLCRAWGGTCGAALTGTSISQVPRRLPRGLHCSAAAHSSEQSLVPSPPEPRQRPTKALVPFEDLFGQAPGGERDKASFLQTVQKFAEHSVRKRGHIDFIYLALRKMREYGVERDLAVYNQLLNIFPKEVFRPRNIIQRIFVHYPRQQECGIAVLEQMENHGVMPNKETEFLLIQIFGRKSYPMLKLVRLKLWFPRFMNVNPFPVPRDLPQDPVELAMFGLRHMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPMQLDLEYVRSGWDNYEFDINEVEEGPVFAMCMAGAHDQATMAKWIQGLQETNPTLAQIPVVFRLAGSTRELQTSSAGLEEPPLPEDHQEEDDNLQRQQQGQS

Target ORF information:

RefSeq Version	NM_016581.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ECSIT signalling integrator (ECSIT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016581.4

ORF Insert Sequence:

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTCC CTCGCCGGCT CCCTCGGGGC 
CTCCACTGCA GCGCAGCTGC CCATAGCTCT GAACAGTCCC TGGTTCCCAG CCCACCGGAA 
CCCCGGCAGA GGCCCACCAA GGCTCTGGTG CCCTTTGAGG ACCTGTTTGG GCAGGCGCCT 
GGTGGGGAAC GGGACAAGGC GAGCTTCCTG CAGACGGTGC AGAAATTTGC GGAGCACAGC 
GTGCGTAAGC GGGGCCACAT TGACTTCATC TACCTGGCCC TGCGCAAGAT GCGGGAGTAT 
GGTGTCGAGC GGGACCTGGC TGTGTACAAC CAGCTGCTCA ACATCTTCCC CAAGGAGGTC 
TTCCGGCCTC GCAACATCAT CCAGCGCATC TTCGTCCACT ACCCTCGGCA GCAGGAGTGT 
GGGATTGCTG TCCTGGAGCA GATGGAGAAC CACGGTGTGA TGCCCAACAA GGAGACGGAG 
TTCCTGCTGA TTCAGATCTT TGGACGCAAA AGCTACCCCA TGCTCAAGTT GGTGCGCCTG 
AAGCTGTGGT TCCCTCGATT CATGAACGTC AACCCCTTCC CAGTGCCCCG GGACCTGCCC 
CAGGACCCTG TGGAGCTGGC CATGTTTGGC CTGCGGCACA TGGAGCCTGA CCTTAGTGCC 
AGGGTCACCA TCTACCAGGT TCCTTTGCCC AAAGACTCAA CAGGTGCAGC AGATCCCCCC 
CAGCCCCACA TCGTAGGAAT CCAGAGTCCC GATCAGCAGG CCGCCCTGGC CCGCCACAAT 
CCAGCCCGGC CTGTCTTTGT TGAGGGCCCC TTCTCCCTGT GGCTCCGCAA CAAGTGTGTG 
TATTACCACA TCCTCAGAGC TGACTTGCTG CCCCCGGAGG AGAGGGAAGT GGAAGAGACG 
CCGGAGGAGT GGAACCTCTA CTACCCGATG CAGCTGGACC TGGAGTATGT GAGGAGTGGC 
TGGGACAACT ACGAGTTTGA CATCAATGAA GTGGAGGAAG GCCCTGTCTT CGCCATGTGC 
ATGGCGGGTG CTCATGACCA GGCGACGATG GCTAAGTGGA TCCAGGGCCT GCAGGAGACC 
AACCCAACCC TGGCCCAGAT CCCCGTGGTC TTCCGCCTCG CCGGGTCCAC CCGGGAGCTC 
CAGACATCCT CTGCAGGGCT GGAGGAGCCG CCCCTGCCCG AGGACCACCA GGAAGAAGAC 
GACAACCTGC AGCGACAGCA GCAGGGCCAG AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECSIT ( NM_016581.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057665.2 Homo sapiens ECSIT signaling integrator (ECSIT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09324
Clone ID Related Accession (Same CDS sequence)	NM_016581.4 , NM_016581.5
Accession Version	NM_016581.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE791936.1 and BC000193.1. On Nov 23, 2018 this sequence version replaced NM_016581.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.755682.1, SRR3476690.782197.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000270517.12/ ENSP00000270517.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTCC CTCGCCGGCT CCCTCGGGGC 
CTCCACTGCA GCGCAGCTGC CCATAGCTCT GAACAGTCCC TGGTTCCCAG CCCACCGGAA 
CCCCGGCAGA GGCCCACCAA GGCTCTGGTG CCCTTTGAGG ACCTGTTTGG GCAGGCGCCT 
GGTGGGGAAC GGGACAAGGC GAGCTTCCTG CAGACGGTGC AGAAATTTGC GGAGCACAGC 
GTGCGTAAGC GGGGCCACAT TGACTTCATC TACCTGGCCC TGCGCAAGAT GCGGGAGTAT 
GGTGTCGAGC GGGACCTGGC TGTGTACAAC CAGCTGCTCA ACATCTTCCC CAAGGAGGTC 
TTCCGGCCTC GCAACATCAT CCAGCGCATC TTCGTCCACT ACCCTCGGCA GCAGGAGTGT 
GGGATTGCTG TCCTGGAGCA GATGGAGAAC CACGGTGTGA TGCCCAACAA GGAGACGGAG 
TTCCTGCTGA TTCAGATCTT TGGACGCAAA AGCTACCCCA TGCTCAAGTT GGTGCGCCTG 
AAGCTGTGGT TCCCTCGATT CATGAACGTC AACCCCTTCC CAGTGCCCCG GGACCTGCCC 
CAGGACCCTG TGGAGCTGGC CATGTTTGGC CTGCGGCACA TGGAGCCTGA CCTTAGTGCC 
AGGGTCACCA TCTACCAGGT TCCTTTGCCC AAAGACTCAA CAGGTGCAGC AGATCCCCCC 
CAGCCCCACA TCGTAGGAAT CCAGAGTCCC GATCAGCAGG CCGCCCTGGC CCGCCACAAT 
CCAGCCCGGC CTGTCTTTGT TGAGGGCCCC TTCTCCCTGT GGCTCCGCAA CAAGTGTGTG 
TATTACCACA TCCTCAGAGC TGACTTGCTG CCCCCGGAGG AGAGGGAAGT GGAAGAGACG 
CCGGAGGAGT GGAACCTCTA CTACCCGATG CAGCTGGACC TGGAGTATGT GAGGAGTGGC 
TGGGACAACT ACGAGTTTGA CATCAATGAA GTGGAGGAAG GCCCTGTCTT CGCCATGTGC 
ATGGCGGGTG CTCATGACCA GGCGACGATG GCTAAGTGGA TCCAGGGCCT GCAGGAGACC 
AACCCAACCC TGGCCCAGAT CCCCGTGGTC TTCCGCCTCG CCGGGTCCAC CCGGGAGCTC 
CAGACATCCT CTGCAGGGCT GGAGGAGCCG CCCCTGCCCG AGGACCACCA GGAAGAAGAC 
GACAACCTGC AGCGACAGCA GCAGGGCCAG AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057665.2
CDS	97..1392
Translation	MSWVQATLLARGLCRAWGGTCGAALTGTSISQVPRRLPRGLHCSAAAHSSEQSLVPSPPEPRQRPTKALVPFEDLFGQAPGGERDKASFLQTVQKFAEHSVRKRGHIDFIYLALRKMREYGVERDLAVYNQLLNIFPKEVFRPRNIIQRIFVHYPRQQECGIAVLEQMENHGVMPNKETEFLLIQIFGRKSYPMLKLVRLKLWFPRFMNVNPFPVPRDLPQDPVELAMFGLRHMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPMQLDLEYVRSGWDNYEFDINEVEEGPVFAMCMAGAHDQATMAKWIQGLQETNPTLAQIPVVFRLAGSTRELQTSSAGLEEPPLPEDHQEEDDNLQRQQQGQS

Target ORF information:

RefSeq Version	NM_016581.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ECSIT signaling integrator (ECSIT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016581.5

ORF Insert Sequence:

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTCC CTCGCCGGCT CCCTCGGGGC 
CTCCACTGCA GCGCAGCTGC CCATAGCTCT GAACAGTCCC TGGTTCCCAG CCCACCGGAA 
CCCCGGCAGA GGCCCACCAA GGCTCTGGTG CCCTTTGAGG ACCTGTTTGG GCAGGCGCCT 
GGTGGGGAAC GGGACAAGGC GAGCTTCCTG CAGACGGTGC AGAAATTTGC GGAGCACAGC 
GTGCGTAAGC GGGGCCACAT TGACTTCATC TACCTGGCCC TGCGCAAGAT GCGGGAGTAT 
GGTGTCGAGC GGGACCTGGC TGTGTACAAC CAGCTGCTCA ACATCTTCCC CAAGGAGGTC 
TTCCGGCCTC GCAACATCAT CCAGCGCATC TTCGTCCACT ACCCTCGGCA GCAGGAGTGT 
GGGATTGCTG TCCTGGAGCA GATGGAGAAC CACGGTGTGA TGCCCAACAA GGAGACGGAG 
TTCCTGCTGA TTCAGATCTT TGGACGCAAA AGCTACCCCA TGCTCAAGTT GGTGCGCCTG 
AAGCTGTGGT TCCCTCGATT CATGAACGTC AACCCCTTCC CAGTGCCCCG GGACCTGCCC 
CAGGACCCTG TGGAGCTGGC CATGTTTGGC CTGCGGCACA TGGAGCCTGA CCTTAGTGCC 
AGGGTCACCA TCTACCAGGT TCCTTTGCCC AAAGACTCAA CAGGTGCAGC AGATCCCCCC 
CAGCCCCACA TCGTAGGAAT CCAGAGTCCC GATCAGCAGG CCGCCCTGGC CCGCCACAAT 
CCAGCCCGGC CTGTCTTTGT TGAGGGCCCC TTCTCCCTGT GGCTCCGCAA CAAGTGTGTG 
TATTACCACA TCCTCAGAGC TGACTTGCTG CCCCCGGAGG AGAGGGAAGT GGAAGAGACG 
CCGGAGGAGT GGAACCTCTA CTACCCGATG CAGCTGGACC TGGAGTATGT GAGGAGTGGC 
TGGGACAACT ACGAGTTTGA CATCAATGAA GTGGAGGAAG GCCCTGTCTT CGCCATGTGC 
ATGGCGGGTG CTCATGACCA GGCGACGATG GCTAAGTGGA TCCAGGGCCT GCAGGAGACC 
AACCCAACCC TGGCCCAGAT CCCCGTGGTC TTCCGCCTCG CCGGGTCCAC CCGGGAGCTC 
CAGACATCCT CTGCAGGGCT GGAGGAGCCG CCCCTGCCCG AGGACCACCA GGAAGAAGAC 
GACAACCTGC AGCGACAGCA GCAGGGCCAG AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECSIT ( NM_001142464.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135936.1 Homo sapiens ECSIT signalling integrator (ECSIT), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09453
Clone ID Related Accession (Same CDS sequence)	NM_001142464.2 , NM_001142464.3
Accession Version	NM_001142464.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE791936.1, BC008279.1 and R46579.1. On Aug 3, 2011 this sequence version replaced NM_001142464.1. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTCC CTCGCCGGCT CCCTCGGGGC 
CTCCACTGCA GCGCAGCTGC CCATAGCTCT GAACAGTCCC TGGTTCCCAG CCCACCGGAA 
CCCCGGCAGA GGCCCACCAA GGCTCTGGTG CCCTTTGAGG ACCTGTTTGG GCAGGCGCCT 
GGTGGGGAAC GGGACAAGGC GAGCTTCCTG CAGACGGTGC AGAAATTTGC GGAGCACAGC 
GTGCGTAAGC GGGGCCACAT TGACTTCATC TACCTGGCCC TGCGCAAGAT GCGGGAGTAT 
GGTGTCGAGC GGGACCTGGC TGTGTACAAC CAGCTGCTCA ACATCTTCCC CAAGGAGGTC 
TTCCGGCCTC GCAACATCAT CCAGCGCATC TTCGTCCACT ACCCTCGGCA GCAGGAGTGT 
GGGATTGCTG TCCTGGAGCA GATGGAGAAC CACGGTGTGA TGCCCAACAA GGAGACGGAG 
TTCCTGCTGA TTCAGATCTT TGGACGCAAA AGCTACCCCA TGCTCAAGTT GGTGCGCCTG 
AAGCTGTGGT TCCCTCGATT CATGAACGTC AACCCCTTCC CAGTGCCCCG GGACCTGCCC 
CAGGACCCTG TGGAGCTGGC CATGTTTGGC CTGCGGCACA TGGAGCCTGA CCTTAGTGCC 
AGGGTCACCA TCTACCAGGT TCCTTTGCCC AAAGACTCAA CAGGTGCAGC AGATCCCCCC 
CAGCCCCACA TCGTAGGAAG TGGAAGAGAC GCCGGAGGAG TGGAACCTCT ACTACCCGAT 
GCAGCTGGAC CTGGAGTATG TGAGGAGTGG CTGGGACAAC TACGAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135936.1
CDS	135..1025
Translation	MSWVQATLLARGLCRAWGGTCGAALTGTSISQVPRRLPRGLHCSAAAHSSEQSLVPSPPEPRQRPTKALVPFEDLFGQAPGGERDKASFLQTVQKFAEHSVRKRGHIDFIYLALRKMREYGVERDLAVYNQLLNIFPKEVFRPRNIIQRIFVHYPRQQECGIAVLEQMENHGVMPNKETEFLLIQIFGRKSYPMLKLVRLKLWFPRFMNVNPFPVPRDLPQDPVELAMFGLRHMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGSGRDAGGVEPLLPDAAGPGVCEEWLGQLRV

Target ORF information:

RefSeq Version	NM_001142464.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ECSIT signalling integrator (ECSIT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142464.2

ORF Insert Sequence:

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTCC CTCGCCGGCT CCCTCGGGGC 
CTCCACTGCA GCGCAGCTGC CCATAGCTCT GAACAGTCCC TGGTTCCCAG CCCACCGGAA 
CCCCGGCAGA GGCCCACCAA GGCTCTGGTG CCCTTTGAGG ACCTGTTTGG GCAGGCGCCT 
GGTGGGGAAC GGGACAAGGC GAGCTTCCTG CAGACGGTGC AGAAATTTGC GGAGCACAGC 
GTGCGTAAGC GGGGCCACAT TGACTTCATC TACCTGGCCC TGCGCAAGAT GCGGGAGTAT 
GGTGTCGAGC GGGACCTGGC TGTGTACAAC CAGCTGCTCA ACATCTTCCC CAAGGAGGTC 
TTCCGGCCTC GCAACATCAT CCAGCGCATC TTCGTCCACT ACCCTCGGCA GCAGGAGTGT 
GGGATTGCTG TCCTGGAGCA GATGGAGAAC CACGGTGTGA TGCCCAACAA GGAGACGGAG 
TTCCTGCTGA TTCAGATCTT TGGACGCAAA AGCTACCCCA TGCTCAAGTT GGTGCGCCTG 
AAGCTGTGGT TCCCTCGATT CATGAACGTC AACCCCTTCC CAGTGCCCCG GGACCTGCCC 
CAGGACCCTG TGGAGCTGGC CATGTTTGGC CTGCGGCACA TGGAGCCTGA CCTTAGTGCC 
AGGGTCACCA TCTACCAGGT TCCTTTGCCC AAAGACTCAA CAGGTGCAGC AGATCCCCCC 
CAGCCCCACA TCGTAGGAAG TGGAAGAGAC GCCGGAGGAG TGGAACCTCT ACTACCCGAT 
GCAGCTGGAC CTGGAGTATG TGAGGAGTGG CTGGGACAAC TACGAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECSIT ( NM_001142464.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135936.1 Homo sapiens ECSIT signaling integrator (ECSIT), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09453
Clone ID Related Accession (Same CDS sequence)	NM_001142464.2 , NM_001142464.3
Accession Version	NM_001142464.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-11
Organism	Homo sapiens(human)
Product	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC008279.1. On May 31, 2019 this sequence version replaced NM_001142464.2. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008279.1, SRR3476690.1119803.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTCC CTCGCCGGCT CCCTCGGGGC 
CTCCACTGCA GCGCAGCTGC CCATAGCTCT GAACAGTCCC TGGTTCCCAG CCCACCGGAA 
CCCCGGCAGA GGCCCACCAA GGCTCTGGTG CCCTTTGAGG ACCTGTTTGG GCAGGCGCCT 
GGTGGGGAAC GGGACAAGGC GAGCTTCCTG CAGACGGTGC AGAAATTTGC GGAGCACAGC 
GTGCGTAAGC GGGGCCACAT TGACTTCATC TACCTGGCCC TGCGCAAGAT GCGGGAGTAT 
GGTGTCGAGC GGGACCTGGC TGTGTACAAC CAGCTGCTCA ACATCTTCCC CAAGGAGGTC 
TTCCGGCCTC GCAACATCAT CCAGCGCATC TTCGTCCACT ACCCTCGGCA GCAGGAGTGT 
GGGATTGCTG TCCTGGAGCA GATGGAGAAC CACGGTGTGA TGCCCAACAA GGAGACGGAG 
TTCCTGCTGA TTCAGATCTT TGGACGCAAA AGCTACCCCA TGCTCAAGTT GGTGCGCCTG 
AAGCTGTGGT TCCCTCGATT CATGAACGTC AACCCCTTCC CAGTGCCCCG GGACCTGCCC 
CAGGACCCTG TGGAGCTGGC CATGTTTGGC CTGCGGCACA TGGAGCCTGA CCTTAGTGCC 
AGGGTCACCA TCTACCAGGT TCCTTTGCCC AAAGACTCAA CAGGTGCAGC AGATCCCCCC 
CAGCCCCACA TCGTAGGAAG TGGAAGAGAC GCCGGAGGAG TGGAACCTCT ACTACCCGAT 
GCAGCTGGAC CTGGAGTATG TGAGGAGTGG CTGGGACAAC TACGAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135936.1
CDS	97..987
Translation	MSWVQATLLARGLCRAWGGTCGAALTGTSISQVPRRLPRGLHCSAAAHSSEQSLVPSPPEPRQRPTKALVPFEDLFGQAPGGERDKASFLQTVQKFAEHSVRKRGHIDFIYLALRKMREYGVERDLAVYNQLLNIFPKEVFRPRNIIQRIFVHYPRQQECGIAVLEQMENHGVMPNKETEFLLIQIFGRKSYPMLKLVRLKLWFPRFMNVNPFPVPRDLPQDPVELAMFGLRHMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGSGRDAGGVEPLLPDAAGPGVCEEWLGQLRV

Target ORF information:

RefSeq Version	NM_001142464.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ECSIT signaling integrator (ECSIT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142464.3

ORF Insert Sequence:

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTCC CTCGCCGGCT CCCTCGGGGC 
CTCCACTGCA GCGCAGCTGC CCATAGCTCT GAACAGTCCC TGGTTCCCAG CCCACCGGAA 
CCCCGGCAGA GGCCCACCAA GGCTCTGGTG CCCTTTGAGG ACCTGTTTGG GCAGGCGCCT 
GGTGGGGAAC GGGACAAGGC GAGCTTCCTG CAGACGGTGC AGAAATTTGC GGAGCACAGC 
GTGCGTAAGC GGGGCCACAT TGACTTCATC TACCTGGCCC TGCGCAAGAT GCGGGAGTAT 
GGTGTCGAGC GGGACCTGGC TGTGTACAAC CAGCTGCTCA ACATCTTCCC CAAGGAGGTC 
TTCCGGCCTC GCAACATCAT CCAGCGCATC TTCGTCCACT ACCCTCGGCA GCAGGAGTGT 
GGGATTGCTG TCCTGGAGCA GATGGAGAAC CACGGTGTGA TGCCCAACAA GGAGACGGAG 
TTCCTGCTGA TTCAGATCTT TGGACGCAAA AGCTACCCCA TGCTCAAGTT GGTGCGCCTG 
AAGCTGTGGT TCCCTCGATT CATGAACGTC AACCCCTTCC CAGTGCCCCG GGACCTGCCC 
CAGGACCCTG TGGAGCTGGC CATGTTTGGC CTGCGGCACA TGGAGCCTGA CCTTAGTGCC 
AGGGTCACCA TCTACCAGGT TCCTTTGCCC AAAGACTCAA CAGGTGCAGC AGATCCCCCC 
CAGCCCCACA TCGTAGGAAG TGGAAGAGAC GCCGGAGGAG TGGAACCTCT ACTACCCGAT 
GCAGCTGGAC CTGGAGTATG TGAGGAGTGG CTGGGACAAC TACGAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECSIT ( NM_001243204.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230133.1 Homo sapiens ECSIT signaling integrator (ECSIT), transcript variant 4, mRNA.

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CloneID	OHu09476
Clone ID Related Accession (Same CDS sequence)	NM_001243204.1
Accession Version	NM_001243204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-11
Organism	Homo sapiens(human)
Product	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK314905.1, BU186092.1, AI336311.1 and R46579.1. On Aug 4, 2011 this sequence version replaced NR_024551.1. Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (4) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314905.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTCC CTCGCCGGCT CCCTCGGGGC 
CTCCACTGCA GCGCAGCTGC CCATAGCTCT GAACAGTCCC TGGTTCCCAG CCCACCGGAA 
CCCCGGCAGA GGCCCACCAA GGCTCTGGTG CCCTTTGAGG ACCTGTTTGG GCAGGCGCCT 
GGTGGGGAAC GGGACAAGGC GAGCTTCCTG CAGACGGTGC AGAAATTTGC GGAGCACAGC 
GTGCGTAAGC GGGGCCACAT TGACTTCATC TACCTGGCCC TGCGCAAGAT GCGGGAGTAT 
GGTGTCGAGC GGGACCTGGC TGTGTACAAC CAGCTGCTCA ACATCTTCCC CAAGGAGGTC 
TTCCGGCCTC GCAACATCAT CCAGCGCATC TTCGTCCACT ACCCTCGGCA GCAGGAGTGT 
GGGATTGCTG TCCTGGAGCA GATGGAGAAC CACGGTGTGA TGCCCAACAA GGAGACGGAG 
TTCCTGCTGA TTCAGATCTT TGGACGCAAA AGCTACCCCA TGCTCAAGTT GGTGCGCCTG 
AAGCTGTGGT TCCCTCGATT CATGAACGTC AACCCCTTCC CAGTGCCCCG GGACCTGCCC 
CAGGACCCTG TGGAGCTGGC CATGTTTGGC CTGCGGCACA TGGAGCCTGA CCTTAGTGCC 
AGGGTCACCA TCTACCAGGT TCCTTTGCCC AAAGACTCAA CAGGTGCAGC AGATCCCCCC 
CAGCCCCACA TCGTAGAGCT GACTTGCTGC CCCCGGAGGA GAGGGAAGTG GAAGAGACGC 
CGGAGGAGTG GAACCTCTAC TACCCGATGC AGCTGGACCT GGAGTATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230133.1
CDS	145..1035
Translation	MSWVQATLLARGLCRAWGGTCGAALTGTSISQVPRRLPRGLHCSAAAHSSEQSLVPSPPEPRQRPTKALVPFEDLFGQAPGGERDKASFLQTVQKFAEHSVRKRGHIDFIYLALRKMREYGVERDLAVYNQLLNIFPKEVFRPRNIIQRIFVHYPRQQECGIAVLEQMENHGVMPNKETEFLLIQIFGRKSYPMLKLVRLKLWFPRFMNVNPFPVPRDLPQDPVELAMFGLRHMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVELTCCPRRRGKWKRRRRSGTSTTRCSWTWSM

Target ORF information:

RefSeq Version	NM_001243204.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ECSIT signaling integrator (ECSIT), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243204.1

ORF Insert Sequence:

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTCC CTCGCCGGCT CCCTCGGGGC 
CTCCACTGCA GCGCAGCTGC CCATAGCTCT GAACAGTCCC TGGTTCCCAG CCCACCGGAA 
CCCCGGCAGA GGCCCACCAA GGCTCTGGTG CCCTTTGAGG ACCTGTTTGG GCAGGCGCCT 
GGTGGGGAAC GGGACAAGGC GAGCTTCCTG CAGACGGTGC AGAAATTTGC GGAGCACAGC 
GTGCGTAAGC GGGGCCACAT TGACTTCATC TACCTGGCCC TGCGCAAGAT GCGGGAGTAT 
GGTGTCGAGC GGGACCTGGC TGTGTACAAC CAGCTGCTCA ACATCTTCCC CAAGGAGGTC 
TTCCGGCCTC GCAACATCAT CCAGCGCATC TTCGTCCACT ACCCTCGGCA GCAGGAGTGT 
GGGATTGCTG TCCTGGAGCA GATGGAGAAC CACGGTGTGA TGCCCAACAA GGAGACGGAG 
TTCCTGCTGA TTCAGATCTT TGGACGCAAA AGCTACCCCA TGCTCAAGTT GGTGCGCCTG 
AAGCTGTGGT TCCCTCGATT CATGAACGTC AACCCCTTCC CAGTGCCCCG GGACCTGCCC 
CAGGACCCTG TGGAGCTGGC CATGTTTGGC CTGCGGCACA TGGAGCCTGA CCTTAGTGCC 
AGGGTCACCA TCTACCAGGT TCCTTTGCCC AAAGACTCAA CAGGTGCAGC AGATCCCCCC 
CAGCCCCACA TCGTAGAGCT GACTTGCTGC CCCCGGAGGA GAGGGAAGTG GAAGAGACGC 
CGGAGGAGTG GAACCTCTAC TACCCGATGC AGCTGGACCT GGAGTATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECSIT ( NM_001142465.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135937.1 Homo sapiens ECSIT signalling integrator (ECSIT), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09506
Clone ID Related Accession (Same CDS sequence)	NM_001142465.2 , NM_001142465.3
Accession Version	NM_001142465.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE791936.1, AI743680.1 and R46579.1. On Aug 3, 2011 this sequence version replaced NM_001142465.1. Transcript Variant: This variant (3) lacks two consecutive exons in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.339393.1, SRR1163658.373488.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTTC CTTTGCCCAA AGACTCAACA 
GGTGCAGCAG ATCCCCCCCA GCCCCACATC GTAGGAATCC AGAGTCCCGA TCAGCAGGCC 
GCCCTGGCCC GCCACAATCC AGCCCGGCCT GTCTTTGTTG AGGGCCCCTT CTCCCTGTGG 
CTCCGCAACA AGTGTGTGTA TTACCACATC CTCAGAGCTG ACTTGCTGCC CCCGGAGGAG 
AGGGAAGTGG AAGAGACGCC GGAGGAGTGG AACCTCTACT ACCCGATGCA GCTGGACCTG 
GAGTATGTGA GGAGTGGCTG GGACAACTAC GAGTTTGACA TCAATGAAGT GGAGGAAGGC 
CCTGTCTTCG CCATGTGCAT GGCGGGTGCT CATGACCAGG CGACGATGGC TAAGTGGATC 
CAGGGCCTGC AGGAGACCAA CCCAACCCTG GCCCAGATCC CCGTGGTCTT CCGCCTCGCC 
GGGTCCACCC GGGAGCTCCA GACATCCTCT GCAGGGCTGG AGGAGCCGCC CCTGCCCGAG 
GACCACCAGG AAGAAGACGA CAACCTGCAG CGACAGCAGC AGGGCCAGAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135937.1
CDS	135..788
Translation	MSWVQATLLARGLCRAWGGTCGAALTGTSISQVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPMQLDLEYVRSGWDNYEFDINEVEEGPVFAMCMAGAHDQATMAKWIQGLQETNPTLAQIPVVFRLAGSTRELQTSSAGLEEPPLPEDHQEEDDNLQRQQQGQS

Target ORF information:

RefSeq Version	NM_001142465.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ECSIT signalling integrator (ECSIT), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142465.2

ORF Insert Sequence:

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTTC CTTTGCCCAA AGACTCAACA 
GGTGCAGCAG ATCCCCCCCA GCCCCACATC GTAGGAATCC AGAGTCCCGA TCAGCAGGCC 
GCCCTGGCCC GCCACAATCC AGCCCGGCCT GTCTTTGTTG AGGGCCCCTT CTCCCTGTGG 
CTCCGCAACA AGTGTGTGTA TTACCACATC CTCAGAGCTG ACTTGCTGCC CCCGGAGGAG 
AGGGAAGTGG AAGAGACGCC GGAGGAGTGG AACCTCTACT ACCCGATGCA GCTGGACCTG 
GAGTATGTGA GGAGTGGCTG GGACAACTAC GAGTTTGACA TCAATGAAGT GGAGGAAGGC 
CCTGTCTTCG CCATGTGCAT GGCGGGTGCT CATGACCAGG CGACGATGGC TAAGTGGATC 
CAGGGCCTGC AGGAGACCAA CCCAACCCTG GCCCAGATCC CCGTGGTCTT CCGCCTCGCC 
GGGTCCACCC GGGAGCTCCA GACATCCTCT GCAGGGCTGG AGGAGCCGCC CCTGCCCGAG 
GACCACCAGG AAGAAGACGA CAACCTGCAG CGACAGCAGC AGGGCCAGAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECSIT ( NM_001142465.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001135937.1 Homo sapiens ECSIT signaling integrator (ECSIT), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09506
Clone ID Related Accession (Same CDS sequence)	NM_001142465.2 , NM_001142465.3
Accession Version	NM_001142465.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-11
Organism	Homo sapiens(human)
Product	evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BE791936.1 and AI743680.1. On Jun 1, 2019 this sequence version replaced NM_001142465.2. Transcript Variant: This variant (3) lacks two consecutive exons in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279842.6353.1, SRR7410570.650916.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTTC CTTTGCCCAA AGACTCAACA 
GGTGCAGCAG ATCCCCCCCA GCCCCACATC GTAGGAATCC AGAGTCCCGA TCAGCAGGCC 
GCCCTGGCCC GCCACAATCC AGCCCGGCCT GTCTTTGTTG AGGGCCCCTT CTCCCTGTGG 
CTCCGCAACA AGTGTGTGTA TTACCACATC CTCAGAGCTG ACTTGCTGCC CCCGGAGGAG 
AGGGAAGTGG AAGAGACGCC GGAGGAGTGG AACCTCTACT ACCCGATGCA GCTGGACCTG 
GAGTATGTGA GGAGTGGCTG GGACAACTAC GAGTTTGACA TCAATGAAGT GGAGGAAGGC 
CCTGTCTTCG CCATGTGCAT GGCGGGTGCT CATGACCAGG CGACGATGGC TAAGTGGATC 
CAGGGCCTGC AGGAGACCAA CCCAACCCTG GCCCAGATCC CCGTGGTCTT CCGCCTCGCC 
GGGTCCACCC GGGAGCTCCA GACATCCTCT GCAGGGCTGG AGGAGCCGCC CCTGCCCGAG 
GACCACCAGG AAGAAGACGA CAACCTGCAG CGACAGCAGC AGGGCCAGAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135937.1
CDS	97..750
Translation	MSWVQATLLARGLCRAWGGTCGAALTGTSISQVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPMQLDLEYVRSGWDNYEFDINEVEEGPVFAMCMAGAHDQATMAKWIQGLQETNPTLAQIPVVFRLAGSTRELQTSSAGLEEPPLPEDHQEEDDNLQRQQQGQS

Target ORF information:

RefSeq Version	NM_001142465.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ECSIT signaling integrator (ECSIT), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142465.3

ORF Insert Sequence:

ATGAGCTGGG TCCAGGCCAC CCTACTGGCC CGAGGCCTCT GTAGGGCCTG GGGAGGCACC 
TGCGGGGCCG CCCTCACAGG AACCTCCATC TCTCAGGTTC CTTTGCCCAA AGACTCAACA 
GGTGCAGCAG ATCCCCCCCA GCCCCACATC GTAGGAATCC AGAGTCCCGA TCAGCAGGCC 
GCCCTGGCCC GCCACAATCC AGCCCGGCCT GTCTTTGTTG AGGGCCCCTT CTCCCTGTGG 
CTCCGCAACA AGTGTGTGTA TTACCACATC CTCAGAGCTG ACTTGCTGCC CCCGGAGGAG 
AGGGAAGTGG AAGAGACGCC GGAGGAGTGG AACCTCTACT ACCCGATGCA GCTGGACCTG 
GAGTATGTGA GGAGTGGCTG GGACAACTAC GAGTTTGACA TCAATGAAGT GGAGGAAGGC 
CCTGTCTTCG CCATGTGCAT GGCGGGTGCT CATGACCAGG CGACGATGGC TAAGTGGATC 
CAGGGCCTGC AGGAGACCAA CCCAACCCTG GCCCAGATCC CCGTGGTCTT CCGCCTCGCC 
GGGTCCACCC GGGAGCTCCA GACATCCTCT GCAGGGCTGG AGGAGCCGCC CCTGCCCGAG 
GACCACCAGG AAGAAGACGA CAACCTGCAG CGACAGCAGC AGGGCCAGAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Recurrent ECSIT mutation encoding V140A triggers hyperinflammation and promotes hemophagocytic syndrome in extranodal NK/T cell lymphoma.
Nature medicine(2018 Jan)
Wen H,Ma H,Cai Q,Lin S,Lei X,He B,Wu S,Wang Z,Gao Y,Liu W,Liu W,Tao Q,Long Z,Yan M,Li D,Kelley KW,Yang Y,Huang H,Liu Q

Hepatitis B virus X protein increases the IL-1?-induced NF-?B activation via interaction with evolutionarily conserved signaling intermediate in Toll pathways (ECSIT).
Virus research195236-45(2015 Jan)
Chen WN,Liu LL,Jiao BY,Lin WS,Lin XJ,Lin X

Ubiquitination of ECSIT is crucial for the activation of p65/p50 NF-?Bs in Toll-like receptor 4 signaling.
Molecular biology of the cell26(1)151-60(2015 Jan)
Mi Wi S,Park J,Shim JH,Chun E,Lee KY

ECSIT bridges RIG-I-like receptors to VISA in signaling events of innate antiviral responses.
Journal of innate immunity7(2)153-64(2015)
Lei CQ,Zhang Y,Li M,Jiang LQ,Zhong B,Kim YH,Shu HB

TAK1-ECSIT-TRAF6 complex plays a key role in the TLR4 signal to activate NF-?B.
The Journal of biological chemistry289(51)35205-14(2014 Dec)
Wi SM,Moon G,Kim J,Kim ST,Shim JH,Chun E,Lee KY

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ECSIT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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