MRPL27 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPL27
Entrez Gene ID	51264
Full Name	mitochondrial ribosomal protein L27
Synonyms	L27mt
General protein information	Preferred Names mitochondrial ribosomal protein L27 Names 39S ribosomal protein L27, mitochondrial MRP-L27 mitochondrial large ribosomal subunit protein bL27m
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.33
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008].
Disorder MIM:	611837

mRNA and Protein(s)

mRNA	Protein	Name
NM_016504.2	NP_057588.1	39S ribosomal protein L27, mitochondrial
NM_016504.3	NP_057588.1	39S ribosomal protein L27, mitochondrial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MRPL27	XP_003315660.1
Rattus norvegicus (Norway rat)	Mrpl27	NP_001099301.1
Danio rerio (zebrafish)	mrpl27	NP_001018516.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002470	XP_312468.5
Gallus gallus (chicken)	MRPL27	XP_001232140.1
Drosophila melanogaster (fruit fly)	mRpL27	NP_001260028.1
Canis lupus familiaris (dog)	MRPL27	XP_548198.3
Xenopus tropicalis (tropical clawed frog)	mrpl27	NP_001016630.1
Homo sapiens (human)	MRPL27	NP_057588.1
Bos taurus (cattle)	MRPL27	NP_001032530.1
Mus musculus (house mouse)	Mrpl27	NP_444391.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Brown A, Rathore S, Kimanius D, Aibara S, Bai XC, Rorbach J, Amunts A, Ramakrishnan V
Nature structural & molecular biology24(10)866-869(2017 Oct)

Structure of the large ribosomal subunit from human mitochondria.
Brown A, Amunts A, Bai XC, Sugimoto Y, Edwards PC, Murshudov G, Scheres SHW, Ramakrishnan V
Science (New York, N.Y.)346(6210)718-722(2014 Nov)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following MRPL27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPL27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08957	NM_016504.3 Latest version!	Homo sapiens mitochondrial ribosomal protein L27 (MRPL27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu08957	NM_016504.2	Homo sapiens mitochondrial ribosomal protein L27 (MRPL27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPL27 ( NM_016504.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_057588.1 Homo sapiens mitochondrial ribosomal protein L27 (MRPL27), mRNA.

Price & Availability↑

CloneID	OHu08957
Clone ID Related Accession (Same CDS sequence)	NM_016504.2 , NM_016504.3
Accession Version	NM_016504.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	39S ribosomal protein L27, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315758.1 and AF151084.1. On Aug 29, 2002 this sequence version replaced NM_016504.1. ##Evidence-Data-START## Transcript exon combination :: AF151084.1, ERR279842.7709.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153733, SAMEA2153932 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG TGGTGTTGGC GCTGAGGACC CGGACAGCCG TTACATCCTT GCTAAGCCCC 
ACTCCGGCTA CAGCTCTTGC TGTCAGATAC GCATCCAAGA AGTCGGGTGG TAGCTCCAAA 
AACCTCGGTG GAAAGTCATC AGGCAGACGC CAAGGCATTA AGAAAATGGA AGGTCACTAT 
GTTCATGCTG GGAACATCAT TGCAACACAG CGCCATTTCC GCTGGCACCC AGGTGCCCAT 
GTGGGTGTTG GGAAGAATAA ATGTCTGTAT GCCCTGGAAG AGGGGATAGT CCGCTACACT 
AAGGAGGTCT ACGTGCCTCA TCCCAGAAAC ACGGAGGCTG TGGATCTGAT CACCAGGCTG 
CCCAAGGGTG CTGTGCTCTA CAAGACTTTT GTCCACGTGG TTCCTGCCAA GCCTGAGGGC 
ACCTTCAAAC TGGTAGCTAT GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057588.1
CDS	32..478
Translation	MASVVLALRTRTAVTSLLSPTPATALAVRYASKKSGGSSKNLGGKSSGRRQGIKKMEGHYVHAGNIIATQRHFRWHPGAHVGVGKNKCLYALEEGIVRYTKEVYVPHPRNTEAVDLITRLPKGAVLYKTFVHVVPAKPEGTFKLVAML

Target ORF information:

RefSeq Version	NM_016504.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L27 (MRPL27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016504.2

ORF Insert Sequence:

ATGGCGTCGG TGGTGTTGGC GCTGAGGACC CGGACAGCCG TTACATCCTT GCTAAGCCCC 
ACTCCGGCTA CAGCTCTTGC TGTCAGATAC GCATCCAAGA AGTCGGGTGG TAGCTCCAAA 
AACCTCGGTG GAAAGTCATC AGGCAGACGC CAAGGCATTA AGAAAATGGA AGGTCACTAT 
GTTCATGCTG GGAACATCAT TGCAACACAG CGCCATTTCC GCTGGCACCC AGGTGCCCAT 
GTGGGTGTTG GGAAGAATAA ATGTCTGTAT GCCCTGGAAG AGGGGATAGT CCGCTACACT 
AAGGAGGTCT ACGTGCCTCA TCCCAGAAAC ACGGAGGCTG TGGATCTGAT CACCAGGCTG 
CCCAAGGGTG CTGTGCTCTA CAAGACTTTT GTCCACGTGG TTCCTGCCAA GCCTGAGGGC 
ACCTTCAAAC TGGTAGCTAT GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL27 ( NM_016504.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057588.1 Homo sapiens mitochondrial ribosomal protein L27 (MRPL27), mRNA.

Price & Availability↑

CloneID	OHu08957
Clone ID Related Accession (Same CDS sequence)	NM_016504.2 , NM_016504.3
Accession Version	NM_016504.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	39S ribosomal protein L27, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF151084.1. On Nov 23, 2018 this sequence version replaced NM_016504.2. ##Evidence-Data-START## Transcript exon combination :: AF151084.1, SRR1163658.334646.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153932, SAMN03267769 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000225969.9/ ENSP00000225969.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGTCGG TGGTGTTGGC GCTGAGGACC CGGACAGCCG TTACATCCTT GCTAAGCCCC 
ACTCCGGCTA CAGCTCTTGC TGTCAGATAC GCATCCAAGA AGTCGGGTGG TAGCTCCAAA 
AACCTCGGTG GAAAGTCATC AGGCAGACGC CAAGGCATTA AGAAAATGGA AGGTCACTAT 
GTTCATGCTG GGAACATCAT TGCAACACAG CGCCATTTCC GCTGGCACCC AGGTGCCCAT 
GTGGGTGTTG GGAAGAATAA ATGTCTGTAT GCCCTGGAAG AGGGGATAGT CCGCTACACT 
AAGGAGGTCT ACGTGCCTCA TCCCAGAAAC ACGGAGGCTG TGGATCTGAT CACCAGGCTG 
CCCAAGGGTG CTGTGCTCTA CAAGACTTTT GTCCACGTGG TTCCTGCCAA GCCTGAGGGC 
ACCTTCAAAC TGGTAGCTAT GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057588.1
CDS	15..461
Translation	MASVVLALRTRTAVTSLLSPTPATALAVRYASKKSGGSSKNLGGKSSGRRQGIKKMEGHYVHAGNIIATQRHFRWHPGAHVGVGKNKCLYALEEGIVRYTKEVYVPHPRNTEAVDLITRLPKGAVLYKTFVHVVPAKPEGTFKLVAML

Target ORF information:

RefSeq Version	NM_016504.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L27 (MRPL27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016504.3

ORF Insert Sequence:

ATGGCGTCGG TGGTGTTGGC GCTGAGGACC CGGACAGCCG TTACATCCTT GCTAAGCCCC 
ACTCCGGCTA CAGCTCTTGC TGTCAGATAC GCATCCAAGA AGTCGGGTGG TAGCTCCAAA 
AACCTCGGTG GAAAGTCATC AGGCAGACGC CAAGGCATTA AGAAAATGGA AGGTCACTAT 
GTTCATGCTG GGAACATCAT TGCAACACAG CGCCATTTCC GCTGGCACCC AGGTGCCCAT 
GTGGGTGTTG GGAAGAATAA ATGTCTGTAT GCCCTGGAAG AGGGGATAGT CCGCTACACT 
AAGGAGGTCT ACGTGCCTCA TCCCAGAAAC ACGGAGGCTG TGGATCTGAT CACCAGGCTG 
CCCAAGGGTG CTGTGCTCTA CAAGACTTTT GTCCACGTGG TTCCTGCCAA GCCTGAGGGC 
ACCTTCAAAC TGGTAGCTAT GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Nature structural & molecular biology24(10)866-869(2017 Oct)
Brown A,Rathore S,Kimanius D,Aibara S,Bai XC,Rorbach J,Amunts A,Ramakrishnan V

Structure of the large ribosomal subunit from human mitochondria.
Science (New York, N.Y.)346(6210)718-722(2014 Nov)
Brown A,Amunts A,Bai XC,Sugimoto Y,Edwards PC,Murshudov G,Scheres SHW,Ramakrishnan V

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

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MRPL27 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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