PCSK2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCSK2
Entrez Gene ID	5126
Full Name	proprotein convertase subtilisin/kexin type 2
Synonyms	NEC 2,NEC-2,NEC2,PC2,SPC2
General protein information	Preferred Names proprotein convertase subtilisin/kexin type 2 Names neuroendocrine convertase 2 KEX2-like endoprotease 2 prohormone convertase 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20p12.1
Summary	This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The protein undergoes an initial autocatalytic processing event and interacts with a neuroendocrine secretory protein in the ER, exits the ER and sorts to secretory granules, where it is cleaved and catalytically activated during intracellular transport. The encoded protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Single nucleotide polymorphisms in this gene may increase susceptibility to myocardial infarction and type 2 diabetes. This gene may also play a role in tumor development and progression. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2014].
Disorder MIM:	162151

mRNA and Protein(s)

mRNA	Protein	Name
NM_001201529.2	NP_001188458.1	neuroendocrine convertase 2 isoform 2 preproprotein
NM_001201529.3	NP_001188458.1	neuroendocrine convertase 2 isoform 2 preproprotein
NM_002594.4	NP_002585.2	neuroendocrine convertase 2 isoform 1 preproprotein
NM_002594.5	NP_002585.2	neuroendocrine convertase 2 isoform 1 preproprotein
NM_001201528.1	NP_001188457.1	neuroendocrine convertase 2 isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	PCSK2	NP_002585.2
Danio rerio (zebrafish)	pcsk2	NP_001135738.1
Drosophila melanogaster (fruit fly)	amon	NP_477318.1
Xenopus tropicalis (tropical clawed frog)	pcsk2	NP_001090660.1
Pan troglodytes (chimpanzee)	PCSK2	XP_001138346.1
	PCSK2	XP_001087532.1
Rattus norvegicus (Norway rat)	Pcsk2	NP_036878.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002176	XP_308012.5
Canis lupus familiaris (dog)	PCSK2	XP_003433232.1
Bos taurus (cattle)	PCSK2	NP_776838.1
Caenorhabditis elegans (roundworm)	egl-3	NP_001023732.1
Mus musculus (house mouse)	Pcsk2	NP_032818.1
Gallus gallus (chicken)	PCSK2	XP_004940271.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional analysis of PCSK2 coding variants: A founder effect in the Old Order Amish population.
Winters A, Ramos-Molina B, Jarvela TS, Yerges-Armstrong L, Pollin TI, Lindberg I
Diabetes research and clinical practice13182-90(2017 Sep)

Genetic polymorphisms of PCSK2 are associated with glucose homeostasis and progression to type 2 diabetes in a Chinese population.
Chang TJ, Chiu YF, Sheu WH, Shih KC, Hwu CM, Quertermous T, Jou YS, Kuo SS, Chang YC, Chuang LM
Scientific reports514380(2015 Nov)

Association of genetic variants in SEMA3F, CLEC16A, LAMA3, and PCSK2 with myocardial infarction in Japanese individuals.
Fujimaki T, Kato K, Yokoi K, Oguri M, Yoshida T, Watanabe S, Metoki N, Yoshida H, Satoh K, Aoyagi Y, Nozawa Y, Kimura G, Yamada Y
Atherosclerosis210(2)468-73(2010 Jun)

Association of the proprotein convertase subtilisin/kexin-type 2 (PCSK2) gene with type 2 diabetes in an African American population.
Leak TS, Keene KL, Langefeld CD, Gallagher CJ, Mychaleckyj JC, Freedman BI, Bowden DW, Rich SS, Sale MM
Molecular genetics and metabolism92(1-2)145-50(2007 Sep-Oct)

Mutations within the propeptide, the primary cleavage site or the catalytic site, or deletion of C-terminal sequences, prevents secretion of proPC2 from transfected COS-7 cells.
Taylor NA, Shennan KI, Cutler DF, Docherty K
The Biochemical journal321 ( Pt 2)367-73(1997 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PCSK2 gene polymorphisms are associated with pleiotropic effects on various traits of glucose homeostasis and incident diabetes.
Title: Genetic polymorphisms of PCSK2 are associated with glucose homeostasis and progression to type 2 diabetes in a Chinese population.

FAM20C plays a role in 7B2-mediated proPC2 activation by phosphorylating residue Thr111; and that 7B2 function is regulated by alternative splicing.
Title: Phosphorylation and Alternative Splicing of 7B2 Reduce Prohormone Convertase 2 Activation.

in the processing of hypothalamic neuropeptides in huntington disease may partially arise from decreased PC1/3 and PC2 expressions.
Title: Decreased hypothalamic prohormone convertase expression in huntington disease patients.

variant of the PCSK2 gene was associated with reduced glucose-stimulated insulin secretion, but also with lower glucagon levels, which could potentially counteract the effects of decreased insulin secretion on the risk of type 2 diabetes
Title: Effect of a common variant of the PCSK2 gene on reduced insulin secretion.

The association of risk allele rs2021785 at PCSK2 with type 2 diabetes exists in a Han Chinese population
Title: Association of type 2 diabetes susceptibility genes (TCF7L2, SLC30A8, PCSK1 and PCSK2) and proinsulin conversion in a Chinese population.

The following PCSK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCSK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09811	NM_002594.5 Latest version!	Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu12092	NM_001201528.1 Latest version!	Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu09858	NM_001201529.3 Latest version!	Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu09858	NM_001201529.2	Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu09811	NM_002594.4	Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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Reference Sequences (Refseq)

PCSK2 ( NM_002594.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002585.2 Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09811
Clone ID Related Accession (Same CDS sequence)	NM_002594.4 , NM_002594.5
Accession Version	NM_002594.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	neuroendocrine convertase 2 isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY137524.1, BC001905.1, BC005815.2, BC040546.1, DA148468.1, CD519990.1 and BP384064.1. On Feb 9, 2014 this sequence version replaced NM_002594.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.4612.1, SRR1660809.129564.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAGGGTG GTTGTGTCTC CCAGTGGAAG GCGGCCGCCG GGTTCCTCTT CTGTGTCATG 
GTTTTTGCAT CTGCTGAGCG ACCGGTCTTC ACGAATCATT TTCTTGTGGA GTTGCATAAA 
GGGGGAGAGG ACAAAGCTCG CCAAGTTGCA GCAGAACACG GCTTTGGAGT CCGAAAGCTT 
CCCTTTGCTG AAGGTCTGTA CCACTTTTAT CACAATGGCC TTGCAAAGGC CAAGAGAAGA 
CGCAGCCTAC ACCACAAGCA GCAGCTGGAG AGAGACCCCA GGGTAAAGAT GGCTTTGCAG 
CAGGAAGGAT TTGACCGAAA AAAGCGAGGT TACAGAGACA TCAATGAGAT CGACATCAAC 
ATGAACGATC CTCTTTTTAC AAAGCAGTGG TATCTGATCA ATACTGGGCA AGCTGATGGC 
ACTCCTGGCC TTGATTTGAA TGTGGCTGAA GCCTGGGAGC TGGGATACAC AGGGAAAGGT 
GTTACCATTG GAATTATGGA TGATGGGATT GACTATCTCC ACCCGGACCT GGCCTCCAAC 
TATAATGCCG AAGCAAGTTA CGACTTCAGC AGCAACGACC CCTATCCTTA CCCTCGGTAC 
ACAGATGACT GGTTTAACAG CCACGGGACC CGATGTGCAG GAGAAGTTTC TGCTGCCGCC 
AACAACAATA TCTGTGGAGT TGGAGTAGCA TACAACTCCA AGGTTGCAGG CATCCGGATG 
CTGGACCAGC CATTCATGAC AGACATCATC GAGGCCTCCT CCATCAGTCA TATGCCACAG 
CTGATTGACA TCTACAGCGC CAGCTGGGGC CCCACAGACA ACGGCAAGAC AGTGGATGGG 
CCCCGGGAGC TCACGCTGCA GGCCATGGCC GATGGCGTGA ACAAGGGCCG CGGCGGCAAA 
GGCAGCATCT ACGTGTGGGC CTCCGGGGAC GGCGGCAGCT ATGACGACTG CAACTGCGAC 
GGCTACGCCT CCAGCATGTG GACCATCTCC ATCAACTCAG CCATCAACGA CGGCAGGACT 
GCCCTGTACG ACGAGAGCTG CTCTTCCACC TTGGCTTCCA CCTTCAGCAA CGGGAGGAAA 
AGGAACCCCG AGGCCGGTGT GGCAACCACA GATTTGTACG GCAACTGCAC TCTGAGGCAT 
TCTGGGACAT CTGCAGCTGC CCCCGAGGCA GCTGGTGTGT TTGCACTGGC TCTGGAGGCT 
AACCTGGGTC TGACCTGGCG GGACATGCAG CATCTGACTG TGCTCACCTC CAAACGGAAC 
CAGCTTCACG ACGAGGTCCA TCAGTGGCGG CGCAATGGGG TCGGCCTGGA ATTTAATCAC 
CTCTTTGGCT ACGGGGTCCT TGATGCAGGT GCCATGGTGA AAATGGCTAA AGACTGGAAA 
ACCGTGCCTG AGAGATTCCA CTGTGTGGGA GGCTCCGTGC AGGACCCTGA GAAAATACCA 
TCCACTGGCA AGTTGGTGCT GACACTCACA ACCGACGCCT GTGAGGGGAA GGAAAATTTT 
GTCCGCTACC TGGAGCATGT CCAGGCTGTC ATCACGGTCA ACGCAACCAG AAGAGGAGAC 
CTGAACATCA ACATGACTTC CCCTATGGGC ACCAAGTCCA TTTTGCTGAG CCGGCGTCCA 
AGGGATGACG ACTCCAAGGT GGGCTTTGAC AAGTGGCCTT TCATGACCAC TCACACGTGG 
GGGGAAGACG CCCGAGGCAC CTGGACCCTG GAGCTGGGAT TTGTCGGCAG CGCCCCGCAG 
AAGGGGGTGC TGAAGGAGTG GACCCTGATG CTGCATGGCA CTCAGAGTGC CCCGTACATC 
GACCAGGTGG TGCGGGATTA CCAGTCCAAG TTGGCCATGT CCAAGAAAGA GGAGCTGGAG 
GAAGAGCTGG ACGAAGCCGT GGAGAGAAGC CTGAAAAGCA TCCTTAACAA GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002585.2
CDS	355..2271
Translation	MKGGCVSQWKAAAGFLFCVMVFASAERPVFTNHFLVELHKGGEDKARQVAAEHGFGVRKLPFAEGLYHFYHNGLAKAKRRRSLHHKQQLERDPRVKMALQQEGFDRKKRGYRDINEIDINMNDPLFTKQWYLINTGQADGTPGLDLNVAEAWELGYTGKGVTIGIMDDGIDYLHPDLASNYNAEASYDFSSNDPYPYPRYTDDWFNSHGTRCAGEVSAAANNNICGVGVAYNSKVAGIRMLDQPFMTDIIEASSISHMPQLIDIYSASWGPTDNGKTVDGPRELTLQAMADGVNKGRGGKGSIYVWASGDGGSYDDCNCDGYASSMWTISINSAINDGRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGVFALALEANLGLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKMAKDWKTVPERFHCVGGSVQDPEKIPSTGKLVLTLTTDACEGKENFVRYLEHVQAVITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGTWTLELGFVGSAPQKGVLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKEELEEELDEAVERSLKSILNKN

Target ORF information:

RefSeq Version	NM_002594.4
Organism	Homo sapiens(human)
Definition	Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002594.4

ORF Insert Sequence:

ATGAAGGGTG GTTGTGTCTC CCAGTGGAAG GCGGCCGCCG GGTTCCTCTT CTGTGTCATG 
GTTTTTGCAT CTGCTGAGCG ACCGGTCTTC ACGAATCATT TTCTTGTGGA GTTGCATAAA 
GGGGGAGAGG ACAAAGCTCG CCAAGTTGCA GCAGAACACG GCTTTGGAGT CCGAAAGCTT 
CCCTTTGCTG AAGGTCTGTA CCACTTTTAT CACAATGGCC TTGCAAAGGC CAAGAGAAGA 
CGCAGCCTAC ACCACAAGCA GCAGCTGGAG AGAGACCCCA GGGTAAAGAT GGCTTTGCAG 
CAGGAAGGAT TTGACCGAAA AAAGCGAGGT TACAGAGACA TCAATGAGAT CGACATCAAC 
ATGAACGATC CTCTTTTTAC AAAGCAGTGG TATCTGATCA ATACTGGGCA AGCTGATGGC 
ACTCCTGGCC TTGATTTGAA TGTGGCTGAA GCCTGGGAGC TGGGATACAC AGGGAAAGGT 
GTTACCATTG GAATTATGGA TGATGGGATT GACTATCTCC ACCCGGACCT GGCCTCCAAC 
TATAATGCCG AAGCAAGTTA CGACTTCAGC AGCAACGACC CCTATCCTTA CCCTCGGTAC 
ACAGATGACT GGTTTAACAG CCACGGGACC CGATGTGCAG GAGAAGTTTC TGCTGCCGCC 
AACAACAATA TCTGTGGAGT TGGAGTAGCA TACAACTCCA AGGTTGCAGG CATCCGGATG 
CTGGACCAGC CATTCATGAC AGACATCATC GAGGCCTCCT CCATCAGTCA TATGCCACAG 
CTGATTGACA TCTACAGCGC CAGCTGGGGC CCCACAGACA ACGGCAAGAC AGTGGATGGG 
CCCCGGGAGC TCACGCTGCA GGCCATGGCC GATGGCGTGA ACAAGGGCCG CGGCGGCAAA 
GGCAGCATCT ACGTGTGGGC CTCCGGGGAC GGCGGCAGCT ATGACGACTG CAACTGCGAC 
GGCTACGCCT CCAGCATGTG GACCATCTCC ATCAACTCAG CCATCAACGA CGGCAGGACT 
GCCCTGTACG ACGAGAGCTG CTCTTCCACC TTGGCTTCCA CCTTCAGCAA CGGGAGGAAA 
AGGAACCCCG AGGCCGGTGT GGCAACCACA GATTTGTACG GCAACTGCAC TCTGAGGCAT 
TCTGGGACAT CTGCAGCTGC CCCCGAGGCA GCTGGTGTGT TTGCACTGGC TCTGGAGGCT 
AACCTGGGTC TGACCTGGCG GGACATGCAG CATCTGACTG TGCTCACCTC CAAACGGAAC 
CAGCTTCACG ACGAGGTCCA TCAGTGGCGG CGCAATGGGG TCGGCCTGGA ATTTAATCAC 
CTCTTTGGCT ACGGGGTCCT TGATGCAGGT GCCATGGTGA AAATGGCTAA AGACTGGAAA 
ACCGTGCCTG AGAGATTCCA CTGTGTGGGA GGCTCCGTGC AGGACCCTGA GAAAATACCA 
TCCACTGGCA AGTTGGTGCT GACACTCACA ACCGACGCCT GTGAGGGGAA GGAAAATTTT 
GTCCGCTACC TGGAGCATGT CCAGGCTGTC ATCACGGTCA ACGCAACCAG AAGAGGAGAC 
CTGAACATCA ACATGACTTC CCCTATGGGC ACCAAGTCCA TTTTGCTGAG CCGGCGTCCA 
AGGGATGACG ACTCCAAGGT GGGCTTTGAC AAGTGGCCTT TCATGACCAC TCACACGTGG 
GGGGAAGACG CCCGAGGCAC CTGGACCCTG GAGCTGGGAT TTGTCGGCAG CGCCCCGCAG 
AAGGGGGTGC TGAAGGAGTG GACCCTGATG CTGCATGGCA CTCAGAGTGC CCCGTACATC 
GACCAGGTGG TGCGGGATTA CCAGTCCAAG TTGGCCATGT CCAAGAAAGA GGAGCTGGAG 
GAAGAGCTGG ACGAAGCCGT GGAGAGAAGC CTGAAAAGCA TCCTTAACAA GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCSK2 ( NM_002594.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002585.2 Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09811
Clone ID Related Accession (Same CDS sequence)	NM_002594.4 , NM_002594.5
Accession Version	NM_002594.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	neuroendocrine convertase 2 isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY137524.1, BC001905.1, BC005815.2, BC040546.1, DA148468.1, CD519990.1 and BP384064.1. On Nov 22, 2018 this sequence version replaced NM_002594.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.72025.1, SRR1660809.4612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262545.7/ ENSP00000262545.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGGGTG GTTGTGTCTC CCAGTGGAAG GCGGCCGCCG GGTTCCTCTT CTGTGTCATG 
GTTTTTGCAT CTGCTGAGCG ACCGGTCTTC ACGAATCATT TTCTTGTGGA GTTGCATAAA 
GGGGGAGAGG ACAAAGCTCG CCAAGTTGCA GCAGAACACG GCTTTGGAGT CCGAAAGCTT 
CCCTTTGCTG AAGGTCTGTA CCACTTTTAT CACAATGGCC TTGCAAAGGC CAAGAGAAGA 
CGCAGCCTAC ACCACAAGCA GCAGCTGGAG AGAGACCCCA GGGTAAAGAT GGCTTTGCAG 
CAGGAAGGAT TTGACCGAAA AAAGCGAGGT TACAGAGACA TCAATGAGAT CGACATCAAC 
ATGAACGATC CTCTTTTTAC AAAGCAGTGG TATCTGATCA ATACTGGGCA AGCTGATGGC 
ACTCCTGGCC TTGATTTGAA TGTGGCTGAA GCCTGGGAGC TGGGATACAC AGGGAAAGGT 
GTTACCATTG GAATTATGGA TGATGGGATT GACTATCTCC ACCCGGACCT GGCCTCCAAC 
TATAATGCCG AAGCAAGTTA CGACTTCAGC AGCAACGACC CCTATCCTTA CCCTCGGTAC 
ACAGATGACT GGTTTAACAG CCACGGGACC CGATGTGCAG GAGAAGTTTC TGCTGCCGCC 
AACAACAATA TCTGTGGAGT TGGAGTAGCA TACAACTCCA AGGTTGCAGG CATCCGGATG 
CTGGACCAGC CATTCATGAC AGACATCATC GAGGCCTCCT CCATCAGTCA TATGCCACAG 
CTGATTGACA TCTACAGCGC CAGCTGGGGC CCCACAGACA ACGGCAAGAC AGTGGATGGG 
CCCCGGGAGC TCACGCTGCA GGCCATGGCC GATGGCGTGA ACAAGGGCCG CGGCGGCAAA 
GGCAGCATCT ACGTGTGGGC CTCCGGGGAC GGCGGCAGCT ATGACGACTG CAACTGCGAC 
GGCTACGCCT CCAGCATGTG GACCATCTCC ATCAACTCAG CCATCAACGA CGGCAGGACT 
GCCCTGTACG ACGAGAGCTG CTCTTCCACC TTGGCTTCCA CCTTCAGCAA CGGGAGGAAA 
AGGAACCCCG AGGCCGGTGT GGCAACCACA GATTTGTACG GCAACTGCAC TCTGAGGCAT 
TCTGGGACAT CTGCAGCTGC CCCCGAGGCA GCTGGTGTGT TTGCACTGGC TCTGGAGGCT 
AACCTGGGTC TGACCTGGCG GGACATGCAG CATCTGACTG TGCTCACCTC CAAACGGAAC 
CAGCTTCACG ACGAGGTCCA TCAGTGGCGG CGCAATGGGG TCGGCCTGGA ATTTAATCAC 
CTCTTTGGCT ACGGGGTCCT TGATGCAGGT GCCATGGTGA AAATGGCTAA AGACTGGAAA 
ACCGTGCCTG AGAGATTCCA CTGTGTGGGA GGCTCCGTGC AGGACCCTGA GAAAATACCA 
TCCACTGGCA AGTTGGTGCT GACACTCACA ACCGACGCCT GTGAGGGGAA GGAAAATTTT 
GTCCGCTACC TGGAGCATGT CCAGGCTGTC ATCACGGTCA ACGCAACCAG AAGAGGAGAC 
CTGAACATCA ACATGACTTC CCCTATGGGC ACCAAGTCCA TTTTGCTGAG CCGGCGTCCA 
AGGGATGACG ACTCCAAGGT GGGCTTTGAC AAGTGGCCTT TCATGACCAC TCACACGTGG 
GGGGAAGACG CCCGAGGCAC CTGGACCCTG GAGCTGGGAT TTGTCGGCAG CGCCCCGCAG 
AAGGGGGTGC TGAAGGAGTG GACCCTGATG CTGCATGGCA CTCAGAGTGC CCCGTACATC 
GACCAGGTGG TGCGGGATTA CCAGTCCAAG TTGGCCATGT CCAAGAAAGA GGAGCTGGAG 
GAAGAGCTGG ACGAAGCCGT GGAGAGAAGC CTGAAAAGCA TCCTTAACAA GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002585.2
CDS	267..2183
Translation	MKGGCVSQWKAAAGFLFCVMVFASAERPVFTNHFLVELHKGGEDKARQVAAEHGFGVRKLPFAEGLYHFYHNGLAKAKRRRSLHHKQQLERDPRVKMALQQEGFDRKKRGYRDINEIDINMNDPLFTKQWYLINTGQADGTPGLDLNVAEAWELGYTGKGVTIGIMDDGIDYLHPDLASNYNAEASYDFSSNDPYPYPRYTDDWFNSHGTRCAGEVSAAANNNICGVGVAYNSKVAGIRMLDQPFMTDIIEASSISHMPQLIDIYSASWGPTDNGKTVDGPRELTLQAMADGVNKGRGGKGSIYVWASGDGGSYDDCNCDGYASSMWTISINSAINDGRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGVFALALEANLGLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKMAKDWKTVPERFHCVGGSVQDPEKIPSTGKLVLTLTTDACEGKENFVRYLEHVQAVITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGTWTLELGFVGSAPQKGVLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKEELEEELDEAVERSLKSILNKN

Target ORF information:

RefSeq Version	NM_002594.5
Organism	Homo sapiens(human)
Definition	Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002594.5

ORF Insert Sequence:

ATGAAGGGTG GTTGTGTCTC CCAGTGGAAG GCGGCCGCCG GGTTCCTCTT CTGTGTCATG 
GTTTTTGCAT CTGCTGAGCG ACCGGTCTTC ACGAATCATT TTCTTGTGGA GTTGCATAAA 
GGGGGAGAGG ACAAAGCTCG CCAAGTTGCA GCAGAACACG GCTTTGGAGT CCGAAAGCTT 
CCCTTTGCTG AAGGTCTGTA CCACTTTTAT CACAATGGCC TTGCAAAGGC CAAGAGAAGA 
CGCAGCCTAC ACCACAAGCA GCAGCTGGAG AGAGACCCCA GGGTAAAGAT GGCTTTGCAG 
CAGGAAGGAT TTGACCGAAA AAAGCGAGGT TACAGAGACA TCAATGAGAT CGACATCAAC 
ATGAACGATC CTCTTTTTAC AAAGCAGTGG TATCTGATCA ATACTGGGCA AGCTGATGGC 
ACTCCTGGCC TTGATTTGAA TGTGGCTGAA GCCTGGGAGC TGGGATACAC AGGGAAAGGT 
GTTACCATTG GAATTATGGA TGATGGGATT GACTATCTCC ACCCGGACCT GGCCTCCAAC 
TATAATGCCG AAGCAAGTTA CGACTTCAGC AGCAACGACC CCTATCCTTA CCCTCGGTAC 
ACAGATGACT GGTTTAACAG CCACGGGACC CGATGTGCAG GAGAAGTTTC TGCTGCCGCC 
AACAACAATA TCTGTGGAGT TGGAGTAGCA TACAACTCCA AGGTTGCAGG CATCCGGATG 
CTGGACCAGC CATTCATGAC AGACATCATC GAGGCCTCCT CCATCAGTCA TATGCCACAG 
CTGATTGACA TCTACAGCGC CAGCTGGGGC CCCACAGACA ACGGCAAGAC AGTGGATGGG 
CCCCGGGAGC TCACGCTGCA GGCCATGGCC GATGGCGTGA ACAAGGGCCG CGGCGGCAAA 
GGCAGCATCT ACGTGTGGGC CTCCGGGGAC GGCGGCAGCT ATGACGACTG CAACTGCGAC 
GGCTACGCCT CCAGCATGTG GACCATCTCC ATCAACTCAG CCATCAACGA CGGCAGGACT 
GCCCTGTACG ACGAGAGCTG CTCTTCCACC TTGGCTTCCA CCTTCAGCAA CGGGAGGAAA 
AGGAACCCCG AGGCCGGTGT GGCAACCACA GATTTGTACG GCAACTGCAC TCTGAGGCAT 
TCTGGGACAT CTGCAGCTGC CCCCGAGGCA GCTGGTGTGT TTGCACTGGC TCTGGAGGCT 
AACCTGGGTC TGACCTGGCG GGACATGCAG CATCTGACTG TGCTCACCTC CAAACGGAAC 
CAGCTTCACG ACGAGGTCCA TCAGTGGCGG CGCAATGGGG TCGGCCTGGA ATTTAATCAC 
CTCTTTGGCT ACGGGGTCCT TGATGCAGGT GCCATGGTGA AAATGGCTAA AGACTGGAAA 
ACCGTGCCTG AGAGATTCCA CTGTGTGGGA GGCTCCGTGC AGGACCCTGA GAAAATACCA 
TCCACTGGCA AGTTGGTGCT GACACTCACA ACCGACGCCT GTGAGGGGAA GGAAAATTTT 
GTCCGCTACC TGGAGCATGT CCAGGCTGTC ATCACGGTCA ACGCAACCAG AAGAGGAGAC 
CTGAACATCA ACATGACTTC CCCTATGGGC ACCAAGTCCA TTTTGCTGAG CCGGCGTCCA 
AGGGATGACG ACTCCAAGGT GGGCTTTGAC AAGTGGCCTT TCATGACCAC TCACACGTGG 
GGGGAAGACG CCCGAGGCAC CTGGACCCTG GAGCTGGGAT TTGTCGGCAG CGCCCCGCAG 
AAGGGGGTGC TGAAGGAGTG GACCCTGATG CTGCATGGCA CTCAGAGTGC CCCGTACATC 
GACCAGGTGG TGCGGGATTA CCAGTCCAAG TTGGCCATGT CCAAGAAAGA GGAGCTGGAG 
GAAGAGCTGG ACGAAGCCGT GGAGAGAAGC CTGAAAAGCA TCCTTAACAA GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCSK2 ( NM_001201528.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001188457.1 Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu12092
Clone ID Related Accession (Same CDS sequence)	NM_001201528.1
Accession Version	NM_001201528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	neuroendocrine convertase 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC040546.1, BC001905.1 and AL031664.1. Transcript Variant: This variant (3) differs in the 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040546.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTTTTG CATCTGCTGA GCGACCGGTC TTCACGAATC ATTTTCTTGT GGAGTTGCAT 
AAAGGGGGAG AGGACAAAGC TCGCCAAGTT GCAGCAGAAC ACGGCTTTGG AGTCCGAAAG 
CTTCCCTTTG CTGAAGGTCT GTACCACTTT TATCACAATG GCCTTGCAAA GGCCAAGAGA 
AGACGCAGCC TACACCACAA GCAGCAGCTG GAGAGAGACC CCAGGGTAAA GATGGCTTTG 
CAGCAGGAAG GATTTGACCG AAAAAAGCGA GGTTACAGAG ACATCAATGA GATCGACATC 
AACATGAACG ATCCTCTTTT TACAAAGCAG TGGTATCTGA TCAATACTGG GCAAGCTGAT 
GGCACTCCTG GCCTTGATTT GAATGTGGCT GAAGCCTGGG AGCTGGGATA CACAGGGAAA 
GGTGTTACCA TTGGAATTAT GGATGATGGG ATTGACTATC TCCACCCGGA CCTGGCCTCC 
AACTATAATG CCGAAGCAAG TTACGACTTC AGCAGCAACG ACCCCTATCC TTACCCTCGG 
TACACAGATG ACTGGTTTAA CAGCCACGGG ACCCGATGTG CAGGAGAAGT TTCTGCTGCC 
GCCAACAACA ATATCTGTGG AGTTGGAGTA GCATACAACT CCAAGGTTGC AGGCATCCGG 
ATGCTGGACC AGCCATTCAT GACAGACATC ATCGAGGCCT CCTCCATCAG TCATATGCCA 
CAGCTGATTG ACATCTACAG CGCCAGCTGG GGCCCCACAG ACAACGGCAA GACAGTGGAT 
GGGCCCCGGG AGCTCACGCT GCAGGCCATG GCCGATGGCG TGAACAAGGG CCGCGGCGGC 
AAAGGCAGCA TCTACGTGTG GGCCTCCGGG GACGGCGGCA GCTATGACGA CTGCAACTGC 
GACGGCTACG CCTCCAGCAT GTGGACCATC TCCATCAACT CAGCCATCAA CGACGGCAGG 
ACTGCCCTGT ACGACGAGAG CTGCTCTTCC ACCTTGGCTT CCACCTTCAG CAACGGGAGG 
AAAAGGAACC CCGAGGCCGG TGTGGCAACC ACAGATTTGT ACGGCAACTG CACTCTGAGG 
CATTCTGGGA CATCTGCAGC TGCCCCCGAG GCAGCTGGTG TGTTTGCACT GGCTCTGGAG 
GCTAACCTGG GTCTGACCTG GCGGGACATG CAGCATCTGA CTGTGCTCAC CTCCAAACGG 
AACCAGCTTC ACGACGAGGT CCATCAGTGG CGGCGCAATG GGGTCGGCCT GGAATTTAAT 
CACCTCTTTG GCTACGGGGT CCTTGATGCA GGTGCCATGG TGAAAATGGC TAAAGACTGG 
AAAACCGTGC CTGAGAGATT CCACTGTGTG GGAGGCTCCG TGCAGGACCC TGAGAAAATA 
CCATCCACTG GCAAGTTGGT GCTGACACTC ACAACCGACG CCTGTGAGGG GAAGGAAAAT 
TTTGTCCGCT ACCTGGAGCA TGTCCAGGCT GTCATCACGG TCAACGCAAC CAGAAGAGGA 
GACCTGAACA TCAACATGAC TTCCCCTATG GGCACCAAGT CCATTTTGCT GAGCCGGCGT 
CCAAGGGATG ACGACTCCAA GGTGGGCTTT GACAAGTGGC CTTTCATGAC CACTCACACG 
TGGGGGGAAG ACGCCCGAGG CACCTGGACC CTGGAGCTGG GATTTGTCGG CAGCGCCCCG 
CAGAAGGGGG TGCTGAAGGA GTGGACCCTG ATGCTGCATG GCACTCAGAG TGCCCCGTAC 
ATCGACCAGG TGGTGCGGGA TTACCAGTCC AAGTTGGCCA TGTCCAAGAA AGAGGAGCTG 
GAGGAAGAGC TGGACGAAGC CGTGGAGAGA AGCCTGAAAA GCATCCTTAA CAAGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188457.1
CDS	307..2166
Translation	MVFASAERPVFTNHFLVELHKGGEDKARQVAAEHGFGVRKLPFAEGLYHFYHNGLAKAKRRRSLHHKQQLERDPRVKMALQQEGFDRKKRGYRDINEIDINMNDPLFTKQWYLINTGQADGTPGLDLNVAEAWELGYTGKGVTIGIMDDGIDYLHPDLASNYNAEASYDFSSNDPYPYPRYTDDWFNSHGTRCAGEVSAAANNNICGVGVAYNSKVAGIRMLDQPFMTDIIEASSISHMPQLIDIYSASWGPTDNGKTVDGPRELTLQAMADGVNKGRGGKGSIYVWASGDGGSYDDCNCDGYASSMWTISINSAINDGRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGVFALALEANLGLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKMAKDWKTVPERFHCVGGSVQDPEKIPSTGKLVLTLTTDACEGKENFVRYLEHVQAVITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGTWTLELGFVGSAPQKGVLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKEELEEELDEAVERSLKSILNKN

Target ORF information:

RefSeq Version	NM_001201528.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201528.1

ORF Insert Sequence:

ATGGTTTTTG CATCTGCTGA GCGACCGGTC TTCACGAATC ATTTTCTTGT GGAGTTGCAT 
AAAGGGGGAG AGGACAAAGC TCGCCAAGTT GCAGCAGAAC ACGGCTTTGG AGTCCGAAAG 
CTTCCCTTTG CTGAAGGTCT GTACCACTTT TATCACAATG GCCTTGCAAA GGCCAAGAGA 
AGACGCAGCC TACACCACAA GCAGCAGCTG GAGAGAGACC CCAGGGTAAA GATGGCTTTG 
CAGCAGGAAG GATTTGACCG AAAAAAGCGA GGTTACAGAG ACATCAATGA GATCGACATC 
AACATGAACG ATCCTCTTTT TACAAAGCAG TGGTATCTGA TCAATACTGG GCAAGCTGAT 
GGCACTCCTG GCCTTGATTT GAATGTGGCT GAAGCCTGGG AGCTGGGATA CACAGGGAAA 
GGTGTTACCA TTGGAATTAT GGATGATGGG ATTGACTATC TCCACCCGGA CCTGGCCTCC 
AACTATAATG CCGAAGCAAG TTACGACTTC AGCAGCAACG ACCCCTATCC TTACCCTCGG 
TACACAGATG ACTGGTTTAA CAGCCACGGG ACCCGATGTG CAGGAGAAGT TTCTGCTGCC 
GCCAACAACA ATATCTGTGG AGTTGGAGTA GCATACAACT CCAAGGTTGC AGGCATCCGG 
ATGCTGGACC AGCCATTCAT GACAGACATC ATCGAGGCCT CCTCCATCAG TCATATGCCA 
CAGCTGATTG ACATCTACAG CGCCAGCTGG GGCCCCACAG ACAACGGCAA GACAGTGGAT 
GGGCCCCGGG AGCTCACGCT GCAGGCCATG GCCGATGGCG TGAACAAGGG CCGCGGCGGC 
AAAGGCAGCA TCTACGTGTG GGCCTCCGGG GACGGCGGCA GCTATGACGA CTGCAACTGC 
GACGGCTACG CCTCCAGCAT GTGGACCATC TCCATCAACT CAGCCATCAA CGACGGCAGG 
ACTGCCCTGT ACGACGAGAG CTGCTCTTCC ACCTTGGCTT CCACCTTCAG CAACGGGAGG 
AAAAGGAACC CCGAGGCCGG TGTGGCAACC ACAGATTTGT ACGGCAACTG CACTCTGAGG 
CATTCTGGGA CATCTGCAGC TGCCCCCGAG GCAGCTGGTG TGTTTGCACT GGCTCTGGAG 
GCTAACCTGG GTCTGACCTG GCGGGACATG CAGCATCTGA CTGTGCTCAC CTCCAAACGG 
AACCAGCTTC ACGACGAGGT CCATCAGTGG CGGCGCAATG GGGTCGGCCT GGAATTTAAT 
CACCTCTTTG GCTACGGGGT CCTTGATGCA GGTGCCATGG TGAAAATGGC TAAAGACTGG 
AAAACCGTGC CTGAGAGATT CCACTGTGTG GGAGGCTCCG TGCAGGACCC TGAGAAAATA 
CCATCCACTG GCAAGTTGGT GCTGACACTC ACAACCGACG CCTGTGAGGG GAAGGAAAAT 
TTTGTCCGCT ACCTGGAGCA TGTCCAGGCT GTCATCACGG TCAACGCAAC CAGAAGAGGA 
GACCTGAACA TCAACATGAC TTCCCCTATG GGCACCAAGT CCATTTTGCT GAGCCGGCGT 
CCAAGGGATG ACGACTCCAA GGTGGGCTTT GACAAGTGGC CTTTCATGAC CACTCACACG 
TGGGGGGAAG ACGCCCGAGG CACCTGGACC CTGGAGCTGG GATTTGTCGG CAGCGCCCCG 
CAGAAGGGGG TGCTGAAGGA GTGGACCCTG ATGCTGCATG GCACTCAGAG TGCCCCGTAC 
ATCGACCAGG TGGTGCGGGA TTACCAGTCC AAGTTGGCCA TGTCCAAGAA AGAGGAGCTG 
GAGGAAGAGC TGGACGAAGC CGTGGAGAGA AGCCTGAAAA GCATCCTTAA CAAGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCSK2 ( NM_001201529.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001188458.1 Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09858
Clone ID Related Accession (Same CDS sequence)	NM_001201529.2 , NM_001201529.3
Accession Version	NM_001201529.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-22
Organism	Homo sapiens(human)
Product	neuroendocrine convertase 2 isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL359511.16, AK294200.1, AL031664.1 and BC040546.1. On Feb 9, 2014 this sequence version replaced NM_001201529.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.21775.1, SRR1803617.13459.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAGGGTG GTTGTGTCTC CCAGTGGAAG GCGGCCGCCG GGTTCCTCTT CTGTGTCATG 
GTTTTTGCAT CTGCTGAGCG ACCGGTCTTC ACGAATCATT TTCTTGTGGA GTTGCATAAA 
GGGGGAGAGG ACAAAGCTCG CCAAGTTGCA GCAGAACACG GCTTTGGAGT CCGAAAGGTA 
AAGATGGCTT TGCAGCAGGA AGGATTTGAC CGAAAAAAGC GAGGTTACAG AGACATCAAT 
GAGATCGACA TCAACATGAA CGATCCTCTT TTTACAAAGC AGTGGTATCT GATCAATACT 
GGGCAAGCTG ATGGCACTCC TGGCCTTGAT TTGAATGTGG CTGAAGCCTG GGAGCTGGGA 
TACACAGGGA AAGGTGTTAC CATTGGAATT ATGGATGATG GGATTGACTA TCTCCACCCG 
GACCTGGCCT CCAACTATAA TGCCGAAGCA AGTTACGACT TCAGCAGCAA CGACCCCTAT 
CCTTACCCTC GGTACACAGA TGACTGGTTT AACAGCCACG GGACCCGATG TGCAGGAGAA 
GTTTCTGCTG CCGCCAACAA CAATATCTGT GGAGTTGGAG TAGCATACAA CTCCAAGGTT 
GCAGGCATCC GGATGCTGGA CCAGCCATTC ATGACAGACA TCATCGAGGC CTCCTCCATC 
AGTCATATGC CACAGCTGAT TGACATCTAC AGCGCCAGCT GGGGCCCCAC AGACAACGGC 
AAGACAGTGG ATGGGCCCCG GGAGCTCACG CTGCAGGCCA TGGCCGATGG CGTGAACAAG 
GGCCGCGGCG GCAAAGGCAG CATCTACGTG TGGGCCTCCG GGGACGGCGG CAGCTATGAC 
GACTGCAACT GCGACGGCTA CGCCTCCAGC ATGTGGACCA TCTCCATCAA CTCAGCCATC 
AACGACGGCA GGACTGCCCT GTACGACGAG AGCTGCTCTT CCACCTTGGC TTCCACCTTC 
AGCAACGGGA GGAAAAGGAA CCCCGAGGCC GGTGTGGCAA CCACAGATTT GTACGGCAAC 
TGCACTCTGA GGCATTCTGG GACATCTGCA GCTGCCCCCG AGGCAGCTGG TGTGTTTGCA 
CTGGCTCTGG AGGCTAACCT GGGTCTGACC TGGCGGGACA TGCAGCATCT GACTGTGCTC 
ACCTCCAAAC GGAACCAGCT TCACGACGAG GTCCATCAGT GGCGGCGCAA TGGGGTCGGC 
CTGGAATTTA ATCACCTCTT TGGCTACGGG GTCCTTGATG CAGGTGCCAT GGTGAAAATG 
GCTAAAGACT GGAAAACCGT GCCTGAGAGA TTCCACTGTG TGGGAGGCTC CGTGCAGGAC 
CCTGAGAAAA TACCATCCAC TGGCAAGTTG GTGCTGACAC TCACAACCGA CGCCTGTGAG 
GGGAAGGAAA ATTTTGTCCG CTACCTGGAG CATGTCCAGG CTGTCATCAC GGTCAACGCA 
ACCAGAAGAG GAGACCTGAA CATCAACATG ACTTCCCCTA TGGGCACCAA GTCCATTTTG 
CTGAGCCGGC GTCCAAGGGA TGACGACTCC AAGGTGGGCT TTGACAAGTG GCCTTTCATG 
ACCACTCACA CGTGGGGGGA AGACGCCCGA GGCACCTGGA CCCTGGAGCT GGGATTTGTC 
GGCAGCGCCC CGCAGAAGGG GGTGCTGAAG GAGTGGACCC TGATGCTGCA TGGCACTCAG 
AGTGCCCCGT ACATCGACCA GGTGGTGCGG GATTACCAGT CCAAGTTGGC CATGTCCAAG 
AAAGAGGAGC TGGAGGAAGA GCTGGACGAA GCCGTGGAGA GAAGCCTGAA AAGCATCCTT 
AACAAGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188458.1
CDS	355..2166
Translation	MKGGCVSQWKAAAGFLFCVMVFASAERPVFTNHFLVELHKGGEDKARQVAAEHGFGVRKVKMALQQEGFDRKKRGYRDINEIDINMNDPLFTKQWYLINTGQADGTPGLDLNVAEAWELGYTGKGVTIGIMDDGIDYLHPDLASNYNAEASYDFSSNDPYPYPRYTDDWFNSHGTRCAGEVSAAANNNICGVGVAYNSKVAGIRMLDQPFMTDIIEASSISHMPQLIDIYSASWGPTDNGKTVDGPRELTLQAMADGVNKGRGGKGSIYVWASGDGGSYDDCNCDGYASSMWTISINSAINDGRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGVFALALEANLGLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKMAKDWKTVPERFHCVGGSVQDPEKIPSTGKLVLTLTTDACEGKENFVRYLEHVQAVITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGTWTLELGFVGSAPQKGVLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKEELEEELDEAVERSLKSILNKN

Target ORF information:

RefSeq Version	NM_001201529.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201529.2

ORF Insert Sequence:

ATGAAGGGTG GTTGTGTCTC CCAGTGGAAG GCGGCCGCCG GGTTCCTCTT CTGTGTCATG 
GTTTTTGCAT CTGCTGAGCG ACCGGTCTTC ACGAATCATT TTCTTGTGGA GTTGCATAAA 
GGGGGAGAGG ACAAAGCTCG CCAAGTTGCA GCAGAACACG GCTTTGGAGT CCGAAAGGTA 
AAGATGGCTT TGCAGCAGGA AGGATTTGAC CGAAAAAAGC GAGGTTACAG AGACATCAAT 
GAGATCGACA TCAACATGAA CGATCCTCTT TTTACAAAGC AGTGGTATCT GATCAATACT 
GGGCAAGCTG ATGGCACTCC TGGCCTTGAT TTGAATGTGG CTGAAGCCTG GGAGCTGGGA 
TACACAGGGA AAGGTGTTAC CATTGGAATT ATGGATGATG GGATTGACTA TCTCCACCCG 
GACCTGGCCT CCAACTATAA TGCCGAAGCA AGTTACGACT TCAGCAGCAA CGACCCCTAT 
CCTTACCCTC GGTACACAGA TGACTGGTTT AACAGCCACG GGACCCGATG TGCAGGAGAA 
GTTTCTGCTG CCGCCAACAA CAATATCTGT GGAGTTGGAG TAGCATACAA CTCCAAGGTT 
GCAGGCATCC GGATGCTGGA CCAGCCATTC ATGACAGACA TCATCGAGGC CTCCTCCATC 
AGTCATATGC CACAGCTGAT TGACATCTAC AGCGCCAGCT GGGGCCCCAC AGACAACGGC 
AAGACAGTGG ATGGGCCCCG GGAGCTCACG CTGCAGGCCA TGGCCGATGG CGTGAACAAG 
GGCCGCGGCG GCAAAGGCAG CATCTACGTG TGGGCCTCCG GGGACGGCGG CAGCTATGAC 
GACTGCAACT GCGACGGCTA CGCCTCCAGC ATGTGGACCA TCTCCATCAA CTCAGCCATC 
AACGACGGCA GGACTGCCCT GTACGACGAG AGCTGCTCTT CCACCTTGGC TTCCACCTTC 
AGCAACGGGA GGAAAAGGAA CCCCGAGGCC GGTGTGGCAA CCACAGATTT GTACGGCAAC 
TGCACTCTGA GGCATTCTGG GACATCTGCA GCTGCCCCCG AGGCAGCTGG TGTGTTTGCA 
CTGGCTCTGG AGGCTAACCT GGGTCTGACC TGGCGGGACA TGCAGCATCT GACTGTGCTC 
ACCTCCAAAC GGAACCAGCT TCACGACGAG GTCCATCAGT GGCGGCGCAA TGGGGTCGGC 
CTGGAATTTA ATCACCTCTT TGGCTACGGG GTCCTTGATG CAGGTGCCAT GGTGAAAATG 
GCTAAAGACT GGAAAACCGT GCCTGAGAGA TTCCACTGTG TGGGAGGCTC CGTGCAGGAC 
CCTGAGAAAA TACCATCCAC TGGCAAGTTG GTGCTGACAC TCACAACCGA CGCCTGTGAG 
GGGAAGGAAA ATTTTGTCCG CTACCTGGAG CATGTCCAGG CTGTCATCAC GGTCAACGCA 
ACCAGAAGAG GAGACCTGAA CATCAACATG ACTTCCCCTA TGGGCACCAA GTCCATTTTG 
CTGAGCCGGC GTCCAAGGGA TGACGACTCC AAGGTGGGCT TTGACAAGTG GCCTTTCATG 
ACCACTCACA CGTGGGGGGA AGACGCCCGA GGCACCTGGA CCCTGGAGCT GGGATTTGTC 
GGCAGCGCCC CGCAGAAGGG GGTGCTGAAG GAGTGGACCC TGATGCTGCA TGGCACTCAG 
AGTGCCCCGT ACATCGACCA GGTGGTGCGG GATTACCAGT CCAAGTTGGC CATGTCCAAG 
AAAGAGGAGC TGGAGGAAGA GCTGGACGAA GCCGTGGAGA GAAGCCTGAA AAGCATCCTT 
AACAAGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCSK2 ( NM_001201529.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001188458.1 Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09858
Clone ID Related Accession (Same CDS sequence)	NM_001201529.2 , NM_001201529.3
Accession Version	NM_001201529.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	neuroendocrine convertase 2 isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL359511.16, AK294200.1, AL031664.1 and BC040546.1. On Jun 1, 2019 this sequence version replaced NM_001201529.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.13459.1, AK294200.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAGGGTG GTTGTGTCTC CCAGTGGAAG GCGGCCGCCG GGTTCCTCTT CTGTGTCATG 
GTTTTTGCAT CTGCTGAGCG ACCGGTCTTC ACGAATCATT TTCTTGTGGA GTTGCATAAA 
GGGGGAGAGG ACAAAGCTCG CCAAGTTGCA GCAGAACACG GCTTTGGAGT CCGAAAGGTA 
AAGATGGCTT TGCAGCAGGA AGGATTTGAC CGAAAAAAGC GAGGTTACAG AGACATCAAT 
GAGATCGACA TCAACATGAA CGATCCTCTT TTTACAAAGC AGTGGTATCT GATCAATACT 
GGGCAAGCTG ATGGCACTCC TGGCCTTGAT TTGAATGTGG CTGAAGCCTG GGAGCTGGGA 
TACACAGGGA AAGGTGTTAC CATTGGAATT ATGGATGATG GGATTGACTA TCTCCACCCG 
GACCTGGCCT CCAACTATAA TGCCGAAGCA AGTTACGACT TCAGCAGCAA CGACCCCTAT 
CCTTACCCTC GGTACACAGA TGACTGGTTT AACAGCCACG GGACCCGATG TGCAGGAGAA 
GTTTCTGCTG CCGCCAACAA CAATATCTGT GGAGTTGGAG TAGCATACAA CTCCAAGGTT 
GCAGGCATCC GGATGCTGGA CCAGCCATTC ATGACAGACA TCATCGAGGC CTCCTCCATC 
AGTCATATGC CACAGCTGAT TGACATCTAC AGCGCCAGCT GGGGCCCCAC AGACAACGGC 
AAGACAGTGG ATGGGCCCCG GGAGCTCACG CTGCAGGCCA TGGCCGATGG CGTGAACAAG 
GGCCGCGGCG GCAAAGGCAG CATCTACGTG TGGGCCTCCG GGGACGGCGG CAGCTATGAC 
GACTGCAACT GCGACGGCTA CGCCTCCAGC ATGTGGACCA TCTCCATCAA CTCAGCCATC 
AACGACGGCA GGACTGCCCT GTACGACGAG AGCTGCTCTT CCACCTTGGC TTCCACCTTC 
AGCAACGGGA GGAAAAGGAA CCCCGAGGCC GGTGTGGCAA CCACAGATTT GTACGGCAAC 
TGCACTCTGA GGCATTCTGG GACATCTGCA GCTGCCCCCG AGGCAGCTGG TGTGTTTGCA 
CTGGCTCTGG AGGCTAACCT GGGTCTGACC TGGCGGGACA TGCAGCATCT GACTGTGCTC 
ACCTCCAAAC GGAACCAGCT TCACGACGAG GTCCATCAGT GGCGGCGCAA TGGGGTCGGC 
CTGGAATTTA ATCACCTCTT TGGCTACGGG GTCCTTGATG CAGGTGCCAT GGTGAAAATG 
GCTAAAGACT GGAAAACCGT GCCTGAGAGA TTCCACTGTG TGGGAGGCTC CGTGCAGGAC 
CCTGAGAAAA TACCATCCAC TGGCAAGTTG GTGCTGACAC TCACAACCGA CGCCTGTGAG 
GGGAAGGAAA ATTTTGTCCG CTACCTGGAG CATGTCCAGG CTGTCATCAC GGTCAACGCA 
ACCAGAAGAG GAGACCTGAA CATCAACATG ACTTCCCCTA TGGGCACCAA GTCCATTTTG 
CTGAGCCGGC GTCCAAGGGA TGACGACTCC AAGGTGGGCT TTGACAAGTG GCCTTTCATG 
ACCACTCACA CGTGGGGGGA AGACGCCCGA GGCACCTGGA CCCTGGAGCT GGGATTTGTC 
GGCAGCGCCC CGCAGAAGGG GGTGCTGAAG GAGTGGACCC TGATGCTGCA TGGCACTCAG 
AGTGCCCCGT ACATCGACCA GGTGGTGCGG GATTACCAGT CCAAGTTGGC CATGTCCAAG 
AAAGAGGAGC TGGAGGAAGA GCTGGACGAA GCCGTGGAGA GAAGCCTGAA AAGCATCCTT 
AACAAGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188458.1
CDS	267..2078
Translation	MKGGCVSQWKAAAGFLFCVMVFASAERPVFTNHFLVELHKGGEDKARQVAAEHGFGVRKVKMALQQEGFDRKKRGYRDINEIDINMNDPLFTKQWYLINTGQADGTPGLDLNVAEAWELGYTGKGVTIGIMDDGIDYLHPDLASNYNAEASYDFSSNDPYPYPRYTDDWFNSHGTRCAGEVSAAANNNICGVGVAYNSKVAGIRMLDQPFMTDIIEASSISHMPQLIDIYSASWGPTDNGKTVDGPRELTLQAMADGVNKGRGGKGSIYVWASGDGGSYDDCNCDGYASSMWTISINSAINDGRTALYDESCSSTLASTFSNGRKRNPEAGVATTDLYGNCTLRHSGTSAAAPEAAGVFALALEANLGLTWRDMQHLTVLTSKRNQLHDEVHQWRRNGVGLEFNHLFGYGVLDAGAMVKMAKDWKTVPERFHCVGGSVQDPEKIPSTGKLVLTLTTDACEGKENFVRYLEHVQAVITVNATRRGDLNINMTSPMGTKSILLSRRPRDDDSKVGFDKWPFMTTHTWGEDARGTWTLELGFVGSAPQKGVLKEWTLMLHGTQSAPYIDQVVRDYQSKLAMSKKEELEEELDEAVERSLKSILNKN

Target ORF information:

RefSeq Version	NM_001201529.3
Organism	Homo sapiens(human)
Definition	Homo sapiens proprotein convertase subtilisin/kexin type 2 (PCSK2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201529.3

ORF Insert Sequence:

ATGAAGGGTG GTTGTGTCTC CCAGTGGAAG GCGGCCGCCG GGTTCCTCTT CTGTGTCATG 
GTTTTTGCAT CTGCTGAGCG ACCGGTCTTC ACGAATCATT TTCTTGTGGA GTTGCATAAA 
GGGGGAGAGG ACAAAGCTCG CCAAGTTGCA GCAGAACACG GCTTTGGAGT CCGAAAGGTA 
AAGATGGCTT TGCAGCAGGA AGGATTTGAC CGAAAAAAGC GAGGTTACAG AGACATCAAT 
GAGATCGACA TCAACATGAA CGATCCTCTT TTTACAAAGC AGTGGTATCT GATCAATACT 
GGGCAAGCTG ATGGCACTCC TGGCCTTGAT TTGAATGTGG CTGAAGCCTG GGAGCTGGGA 
TACACAGGGA AAGGTGTTAC CATTGGAATT ATGGATGATG GGATTGACTA TCTCCACCCG 
GACCTGGCCT CCAACTATAA TGCCGAAGCA AGTTACGACT TCAGCAGCAA CGACCCCTAT 
CCTTACCCTC GGTACACAGA TGACTGGTTT AACAGCCACG GGACCCGATG TGCAGGAGAA 
GTTTCTGCTG CCGCCAACAA CAATATCTGT GGAGTTGGAG TAGCATACAA CTCCAAGGTT 
GCAGGCATCC GGATGCTGGA CCAGCCATTC ATGACAGACA TCATCGAGGC CTCCTCCATC 
AGTCATATGC CACAGCTGAT TGACATCTAC AGCGCCAGCT GGGGCCCCAC AGACAACGGC 
AAGACAGTGG ATGGGCCCCG GGAGCTCACG CTGCAGGCCA TGGCCGATGG CGTGAACAAG 
GGCCGCGGCG GCAAAGGCAG CATCTACGTG TGGGCCTCCG GGGACGGCGG CAGCTATGAC 
GACTGCAACT GCGACGGCTA CGCCTCCAGC ATGTGGACCA TCTCCATCAA CTCAGCCATC 
AACGACGGCA GGACTGCCCT GTACGACGAG AGCTGCTCTT CCACCTTGGC TTCCACCTTC 
AGCAACGGGA GGAAAAGGAA CCCCGAGGCC GGTGTGGCAA CCACAGATTT GTACGGCAAC 
TGCACTCTGA GGCATTCTGG GACATCTGCA GCTGCCCCCG AGGCAGCTGG TGTGTTTGCA 
CTGGCTCTGG AGGCTAACCT GGGTCTGACC TGGCGGGACA TGCAGCATCT GACTGTGCTC 
ACCTCCAAAC GGAACCAGCT TCACGACGAG GTCCATCAGT GGCGGCGCAA TGGGGTCGGC 
CTGGAATTTA ATCACCTCTT TGGCTACGGG GTCCTTGATG CAGGTGCCAT GGTGAAAATG 
GCTAAAGACT GGAAAACCGT GCCTGAGAGA TTCCACTGTG TGGGAGGCTC CGTGCAGGAC 
CCTGAGAAAA TACCATCCAC TGGCAAGTTG GTGCTGACAC TCACAACCGA CGCCTGTGAG 
GGGAAGGAAA ATTTTGTCCG CTACCTGGAG CATGTCCAGG CTGTCATCAC GGTCAACGCA 
ACCAGAAGAG GAGACCTGAA CATCAACATG ACTTCCCCTA TGGGCACCAA GTCCATTTTG 
CTGAGCCGGC GTCCAAGGGA TGACGACTCC AAGGTGGGCT TTGACAAGTG GCCTTTCATG 
ACCACTCACA CGTGGGGGGA AGACGCCCGA GGCACCTGGA CCCTGGAGCT GGGATTTGTC 
GGCAGCGCCC CGCAGAAGGG GGTGCTGAAG GAGTGGACCC TGATGCTGCA TGGCACTCAG 
AGTGCCCCGT ACATCGACCA GGTGGTGCGG GATTACCAGT CCAAGTTGGC CATGTCCAAG 
AAAGAGGAGC TGGAGGAAGA GCTGGACGAA GCCGTGGAGA GAAGCCTGAA AAGCATCCTT 
AACAAGAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional analysis of PCSK2 coding variants: A founder effect in the Old Order Amish population.
Diabetes research and clinical practice13182-90(2017 Sep)
Winters A,Ramos-Molina B,Jarvela TS,Yerges-Armstrong L,Pollin TI,Lindberg I

Genetic polymorphisms of PCSK2 are associated with glucose homeostasis and progression to type 2 diabetes in a Chinese population.
Scientific reports514380(2015 Nov)
Chang TJ,Chiu YF,Sheu WH,Shih KC,Hwu CM,Quertermous T,Jou YS,Kuo SS,Chang YC,Chuang LM

Association of genetic variants in SEMA3F, CLEC16A, LAMA3, and PCSK2 with myocardial infarction in Japanese individuals.
Atherosclerosis210(2)468-73(2010 Jun)
Fujimaki T,Kato K,Yokoi K,Oguri M,Yoshida T,Watanabe S,Metoki N,Yoshida H,Satoh K,Aoyagi Y,Nozawa Y,Kimura G,Yamada Y

Association of the proprotein convertase subtilisin/kexin-type 2 (PCSK2) gene with type 2 diabetes in an African American population.
Molecular genetics and metabolism92(1-2)145-50(2007 Sep-Oct)
Leak TS,Keene KL,Langefeld CD,Gallagher CJ,Mychaleckyj JC,Freedman BI,Bowden DW,Rich SS,Sale MM

Mutations within the propeptide, the primary cleavage site or the catalytic site, or deletion of C-terminal sequences, prevents secretion of proPC2 from transfected COS-7 cells.
The Biochemical journal321 ( Pt 2)367-73(1997 Jan)
Taylor NA,Shennan KI,Cutler DF,Docherty K

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PCSK2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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