TNNI3K cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNNI3K
Entrez Gene ID	51086
Full Name	TNNI3 interacting kinase
Synonyms	CARK,CCDD
General protein information	Preferred Names TNNI3 interacting kinase Names serine/threonine-protein kinase TNNI3K cardiac ankyrin repeat kinase cardiac troponin I-interacting kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p31.1
Summary	This gene encodes a protein that belongs to the MAP kinase kinase kinase (MAPKKK) family of protein kinases. The protein contains ankyrin repeat, protein kinase and serine-rich domains and is thought to play a role in cardiac physiology. [provided by RefSeq, Sep 2012].
Disorder MIM:	613932
Disorder Html:	?Cardiac conduction disease with or without dilated cardiomyopathy, 616117 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_015978.2	NP_057062.1	serine/threonine-protein kinase TNNI3K
NM_015978.3	NP_057062.1	serine/threonine-protein kinase TNNI3K

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TNNI3K	NP_001191741.1
Danio rerio (zebrafish)	tnni3k	NP_001191752.1
	TNNI3K	XP_002801668.1
Gallus gallus (chicken)	TNNI3K	NP_001191745.1
Homo sapiens (human)	TNNI3K	NP_057062.1
Pan troglodytes (chimpanzee)	TNNI3K	NP_001191726.1
Arabidopsis thaliana (thale cress)	VIK	NP_172853.1
Xenopus tropicalis (tropical clawed frog)	tnni3k	NP_001191750.1
Bos taurus (cattle)	TNNI3K	NP_001071476.1
Mus musculus (house mouse)	Tnni3k	NP_796040.3
Rattus norvegicus (Norway rat)	Tnni3k	NP_861434.1
Caenorhabditis elegans (roundworm)	C24A1.3	NP_001021182.1

Gene Ontology
Bibliography

Related articles in PubMed

Whole exome sequencing identifies a novel mutation (c.333?+?2T?>?C) of TNNI3K in a Chinese family with dilated cardiomyopathy and cardiac conduction disease.
Fan LL, Huang H, Jin JY, Li JJ, Chen YQ, Zhao SP, Xiang R
Gene(2018 Jan)

ACE2, CALM3 and TNNI3K polymorphisms as potential disease modifiers in hypertrophic and dilated cardiomyopathies.
Kumar A, Rani B, Sharma R, Kaur G, Prasad R, Bahl A, Khullar M
Molecular and cellular biochemistry438(1-2)167-174(2018 Jan)

Overexpression of Cardiac-Specific Kinase TNNI3K Promotes Mouse Embryonic Stem Cells Differentiation into Cardiomyocytes.
Wang Y, Wang SQ, Wang LP, Yao YH, Ma CY, Ding JF, Ye J, Meng XM, Li JJ, Xu RX
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology41(1)381-398(2017)

Parent-of-origin-specific allelic associations among 106 genomic loci for age at menarche.
Perry JR, Day F, Elks CE, Sulem P, Thompson DJ, Ferreira T, He C, Chasman DI, Esko T, Thorleifsson G, Albrecht E, Ang WQ, Corre T, Cousminer DL, Feenstra B, Franceschini N, Ganna A, Johnson AD, Kjellqvist S, Lunetta KL, McMahon G, Nolte IM, Paternoster L, Porcu E, Smith AV, Stolk L, Teumer A, T?ernikova N, Tikkanen E, Ulivi S, Wagner EK, Amin N, Bierut LJ, Byrne EM, Hottenga JJ, Koller DL, Mangino M, Pers TH, Yerges-Armstrong LM, Zhao JH, Andrulis IL, Anton-Culver H, Atsma F, Bandinelli S, Beckmann MW, Benitez J, Blomqvist C, Bojesen SE, Bolla MK, Bonanni B, Brauch H, Brenner H, Buring JE, Chang-Claude J, Chanock S, Chen J, Chenevix-Trench G, Coll?e JM, Couch FJ, Couper D, Coveillo AD, Cox A, Czene K, D'adamo AP, Smith GD, De Vivo I, Demerath EW, Dennis J, Devilee P, Dieffenbach AK, Dunning AM, Eiriksdottir G, Eriksson JG, Fasching PA, Ferrucci L, Flesch-Janys D, Flyger H, Foroud T, Franke L, Garcia ME, Garc?a-Closas M, Geller F, de Geus EE, Giles GG, Gudbjartsson DF, Gudnason V, Gu?nel P, Guo S, Hall P, Hamann U, Haring R, Hartman CA, Heath AC, Hofman A, Hooning MJ, Hopper JL, Hu FB, Hunter DJ, Karasik D, Kiel DP, Knight JA, Kosma VM, Kutalik Z, Lai S, Lambrechts D, Lindblom A, M?gi R, Magnusson PK, Mannermaa A, Martin NG, Masson G, McArdle PF, McArdle WL, Melbye M, Michailidou K, Mihailov E, Milani L, Milne RL, Nevanlinna H, Neven P, Nohr EA, Oldehinkel AJ, Oostra BA, Palotie A, Peacock M, Pedersen NL, Peterlongo P, Peto J, Pharoah PD, Postma DS, Pouta A, Pylk?s K, Radice P, Ring S, Rivadeneira F, Robino A, Rose LM, Rudolph A, Salomaa V, Sanna S, Schlessinger D, Schmidt MK, Southey MC, Sovio U, Stampfer MJ, St?ckl D, Storniolo AM, Timpson NJ, Tyrer J, Visser JA, Vollenweider P, V?lzke H, Waeber G, Waldenberger M, Wallaschofski H, Wang Q, Willemsen G, Winqvist R, Wolffenbuttel BH, Wright MJ, , , , , , , Boomsma DI, Econs MJ, Khaw KT, Loos RJ, McCarthy MI, Montgomery GW, Rice JP, Streeten EA, Thorsteinsdottir U, van Duijn CM, Alizadeh BZ, Bergmann S, Boerwinkle E, Boyd HA, Crisponi L, Gasparini P, Gieger C, Harris TB, Ingelsson E, J?rvelin MR, Kraft P, Lawlor D, Metspalu A, Pennell CE, Ridker PM, Snieder H, S?rensen TI, Spector TD, Strachan DP, Uitterlinden AG, Wareham NJ, Widen E, Zygmunt M, Murray A, Easton DF, Stefansson K, Murabito JM, Ong KK
Nature514(7520)92-97(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TNNI3K overexpression promoted mouse embryonic stem cells differentiating into beating cardiomyocytes and induced up-regulating expression of cTnT by PKCepsilon signal pathway
Title: Overexpression of Cardiac-Specific Kinase TNNI3K Promotes Mouse Embryonic Stem Cells Differentiation into Cardiomyocytes.

we demonstrate that mutation of TNNI3K, encoding a heart-specific kinase known to modulate murine cardiac conduction and myocardial function, underlies a familial syndrome of electrical and myopathic heart disease in humans.
Title: TNNI3K mutation in familial syndrome of conduction system disease, atrial tachyarrhythmia and dilated cardiomyopathy.

using novel genetic animal models and newly developed small-molecule TNNI3K inhibitors, we demonstrated that TNNI3K-mediated IR injury occurs through impaired mitochondrial function and is in part dependent on p38 MAPK.
Title: Troponin I-interacting protein kinase: a novel cardiac-specific kinase, emerging as a molecular target for the treatment of cardiac disease.

TNNI3K mediates cell signaling to modulate cardiac response to stress.
Title: Overexpression of TNNI3K, a cardiac-specific MAPKKK, promotes cardiac dysfunction.

Results suggest that TNNI3K overexpression induces cardiomyocytes hypertrophy and accelerates hypertrophy in hypertrophic cardiomyocytes.
Title: Adenovirus-mediated overexpression of cardiac troponin I-interacting kinase promotes cardiomyocyte hypertrophy.

The following TNNI3K gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNNI3K cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19579	NM_015978.3 Latest version!	Homo sapiens TNNI3 interacting kinase (TNNI3K), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
View Old Accession Versions >>
OHu19579	NM_015978.2	Homo sapiens TNNI3 interacting kinase (TNNI3K), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNNI3K ( NM_015978.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_057062.1 Homo sapiens TNNI3 interacting kinase (TNNI3K), mRNA.

Price & Availability↑

CloneID	OHu19579
Clone ID Related Accession (Same CDS sequence)	NM_015978.2 , NM_015978.3
Accession Version	NM_015978.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase TNNI3K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX502807.1, BC113539.1, AA758546.1 and BG187210.1. This sequence is a reference standard in the RefSeqGene project. On Dec 4, 2010 this sequence version replaced NM_015978.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF116826.1, AY303691.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAAATT ATAAATCTAG ACCAACCCAA ACTTGTACTG ATGAATGGAA GAAAAAAGTC 
AGTGAATCAT ATGTTATCAC AATAGAAAGA TTAGAAGATG ACCTGCAGAT CAAGGAAAAA 
GAACTGACAG AACTAAGGAA TATATTTGGC TCTGATGAAG CCTTCAGTAA AGTCAATTTA 
AATTACCGCA CTGAAAATGG GCTGTCTCTA CTTCATTTAT GTTGCATTTG TGGAGGCAAG 
AAATCACATA TTCGAACTCT TATGTTGAAA GGGCTCCGCC CATCTCGACT GACAAGAAAT 
GGATTTACAG CCTTGCATTT AGCAGTTTAC AAGGATAATG CAGAATTGAT CACTTCTCTG 
CTTCACAGTG GAGCTGATAT ACAGCAGGTT GGATACGGTG GCCTCACTGC CCTCCATATT 
GCTACAATAG CTGGCCACCT AGAGGCTGCT GATGTGCTGT TGCAACATGG AGCTAATGTC 
AATATTCAAG ATGCAGTTTT TTTCACTCCA TTGCATATTG CAGCGTACTA TGGACATGAA 
CAGGTAACTC GCCTTCTTTT GAAATTTGGT GCTGATGTAA ATGTAAGTGG TGAAGTTGGA 
GATAGACCCC TCCACCTAGC ATCTGCAAAA GGATTCTTGA ATATTGCAAA ACTCTTGATG 
GAAGAAGGCA GCAAAGCAGA TGTGAATGCT CAAGATAATG AAGACCATGT CCCACTCCAT 
TTCTGTTCTC GATTTGGACA CCATGATATA GTTAAGTATC TGCTGCAAAG TGATTTGGAA 
GTTCAACCTC ATGTTGTTAA TATCTATGGA GATACCCCCT TACACCTGGC ATGCTACAAT 
GGCAAATTTG AAGTTGCCAA GGAAATCATC CAAATATCAG GAACAGAAAG TCTGACTAAG 
GAAAACATCT TCAGTGAAAC AGCTTTTCAT AGTGCTTGTA CCTATGGCAA GAGCATTGAC 
CTAGTCAAAT TTCTTCTTGA TCAGAATGTC ATAAACATCA ACCACCAAGG AAGGGATGGG 
CACACTGGAT TACACTCTGC TTGCTACCAC GGTCACATTC GCCTGGTTCA GTTCTTACTG 
GATAATGGAG CTGATATGAA TCTAGTGGCT TGTGATCCCA GCAGGTCTAG TGGTGAAAAA 
GATGAGCAGA CATGTTTGAT GTGGGCTTAT GAAAAAGGGC ATGATGCCAT TGTCACACTC 
CTGAAGCATT ATAAGAGACC ACAAGATGAA TTGCCCTGTA ATGAATATTC TCAGCCTGGA 
GGAGATGGCT CCTATGTGTC TGTTCCATCA CCCTTGGGGA AGATTAAAAG CATGACAAAA 
GAGAAGGCAG ATATTCTCCT CCTAAGAGCT GGATTGCCTT CACATTTCCA TCTTCAGCTC 
TCAGAAATTG AGTTCCATGA GATTATTGGC TCAGGTTCTT TTGGGAAAGT ATATAAAGGA 
CGATGCAGAA ATAAAATAGT GGCTATAAAA CGTTATCGAG CCAATACCTA CTGCTCCAAG 
TCAGATGTGG ATATGTTTTG CCGAGAGGTG TCCATTCTCT GCCAGCTCAA TCATCCCTGC 
GTAATTCAGT TTGTGGGTGC TTGCTTGAAT GATCCCAGCC AGTTTGCCAT TGTCACTCAA 
TACATATCAG GGGGTTCTCT GTTCTCCCTC CTTCATGAGC AGAAGAGGAT TCTTGATTTG 
CAGTCTAAAT TAATTATTGC AGTAGATGTT GCCAAAGGCA TGGAGTACCT TCACAACCTG 
ACACAGCCAA TTATACATCG TGACTTGAAC AGTCACAATA TTCTTCTCTA TGAGGATGGG 
CATGCTGTGG TGGCAGATTT TGGAGAATCA AGATTTCTAC AGTCTCTGGA TGAAGACAAC 
ATGACAAAAC AACCTGGGAA CCTCCGTTGG ATGGCTCCTG AGGTGTTCAC GCAGTGCACT 
CGGTACACCA TCAAAGCAGA TGTCTTCAGC TATGCTCTGT GTCTGTGGGA AATTCTCACT 
GGCGAAATTC CATTCGCTCA TCTCAAGCCA GCGGCTGCGG CAGCAGACAT GGCTTACCAC 
CACATCAGAC CTCCCATTGG CTATTCCATT CCCAAGCCCA TATCATCTCT GCTGATACGA 
GGGTGGAACG CATGTCCTGA AGGAAGACCC GAATTTTCTG AAGTTGTCAT GAAGTTAGAA 
GAGTGTCTCT GCAACATTGA GCTGATGTCT CCTGCATCAA GTAACAGCAG TGGGTCTCTC 
TCACCTTCTT CTTCTTCTGA TTGCCTGGTG AACCGGGGAG GACCTGGCCG GAGTCATGTG 
GCAGCATTAA GAAGTCGTTT CGAATTGGAA TATGCTCTAA ATGCAAGGTC CTATGCTGCT 
TTGTCCCAAA GTGCTGGACA ATATTCCTCT CAAGGTCTGT CTTTGGAGGA GATGAAAAGA 
AGTCTTCAAT ACACACCCAT TGACAAATAT GGCTATGTAT CCGATCCCAT GAGCTCAATG 
CATTTTCATT CTTGCCGAAA TAGTAGCAGC TTTGAGGACA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057062.1
CDS	66..2573
Translation	MGNYKSRPTQTCTDEWKKKVSESYVITIERLEDDLQIKEKELTELRNIFGSDEAFSKVNLNYRTENGLSLLHLCCICGGKKSHIRTLMLKGLRPSRLTRNGFTALHLAVYKDNAELITSLLHSGADIQQVGYGGLTALHIATIAGHLEAADVLLQHGANVNIQDAVFFTPLHIAAYYGHEQVTRLLLKFGADVNVSGEVGDRPLHLASAKGFLNIAKLLMEEGSKADVNAQDNEDHVPLHFCSRFGHHDIVKYLLQSDLEVQPHVVNIYGDTPLHLACYNGKFEVAKEIIQISGTESLTKENIFSETAFHSACTYGKSIDLVKFLLDQNVININHQGRDGHTGLHSACYHGHIRLVQFLLDNGADMNLVACDPSRSSGEKDEQTCLMWAYEKGHDAIVTLLKHYKRPQDELPCNEYSQPGGDGSYVSVPSPLGKIKSMTKEKADILLLRAGLPSHFHLQLSEIEFHEIIGSGSFGKVYKGRCRNKIVAIKRYRANTYCSKSDVDMFCREVSILCQLNHPCVIQFVGACLNDPSQFAIVTQYISGGSLFSLLHEQKRILDLQSKLIIAVDVAKGMEYLHNLTQPIIHRDLNSHNILLYEDGHAVVADFGESRFLQSLDEDNMTKQPGNLRWMAPEVFTQCTRYTIKADVFSYALCLWEILTGEIPFAHLKPAAAAADMAYHHIRPPIGYSIPKPISSLLIRGWNACPEGRPEFSEVVMKLEECLCNIELMSPASSNSSGSLSPSSSSDCLVNRGGPGRSHVAALRSRFELEYALNARSYAALSQSAGQYSSQGLSLEEMKRSLQYTPIDKYGYVSDPMSSMHFHSCRNSSSFEDSS

Target ORF information:

RefSeq Version	NM_015978.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TNNI3 interacting kinase (TNNI3K), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015978.2

ORF Insert Sequence:

ATGGGAAATT ATAAATCTAG ACCAACCCAA ACTTGTACTG ATGAATGGAA GAAAAAAGTC 
AGTGAATCAT ATGTTATCAC AATAGAAAGA TTAGAAGATG ACCTGCAGAT CAAGGAAAAA 
GAACTGACAG AACTAAGGAA TATATTTGGC TCTGATGAAG CCTTCAGTAA AGTCAATTTA 
AATTACCGCA CTGAAAATGG GCTGTCTCTA CTTCATTTAT GTTGCATTTG TGGAGGCAAG 
AAATCACATA TTCGAACTCT TATGTTGAAA GGGCTCCGCC CATCTCGACT GACAAGAAAT 
GGATTTACAG CCTTGCATTT AGCAGTTTAC AAGGATAATG CAGAATTGAT CACTTCTCTG 
CTTCACAGTG GAGCTGATAT ACAGCAGGTT GGATACGGTG GCCTCACTGC CCTCCATATT 
GCTACAATAG CTGGCCACCT AGAGGCTGCT GATGTGCTGT TGCAACATGG AGCTAATGTC 
AATATTCAAG ATGCAGTTTT TTTCACTCCA TTGCATATTG CAGCGTACTA TGGACATGAA 
CAGGTAACTC GCCTTCTTTT GAAATTTGGT GCTGATGTAA ATGTAAGTGG TGAAGTTGGA 
GATAGACCCC TCCACCTAGC ATCTGCAAAA GGATTCTTGA ATATTGCAAA ACTCTTGATG 
GAAGAAGGCA GCAAAGCAGA TGTGAATGCT CAAGATAATG AAGACCATGT CCCACTCCAT 
TTCTGTTCTC GATTTGGACA CCATGATATA GTTAAGTATC TGCTGCAAAG TGATTTGGAA 
GTTCAACCTC ATGTTGTTAA TATCTATGGA GATACCCCCT TACACCTGGC ATGCTACAAT 
GGCAAATTTG AAGTTGCCAA GGAAATCATC CAAATATCAG GAACAGAAAG TCTGACTAAG 
GAAAACATCT TCAGTGAAAC AGCTTTTCAT AGTGCTTGTA CCTATGGCAA GAGCATTGAC 
CTAGTCAAAT TTCTTCTTGA TCAGAATGTC ATAAACATCA ACCACCAAGG AAGGGATGGG 
CACACTGGAT TACACTCTGC TTGCTACCAC GGTCACATTC GCCTGGTTCA GTTCTTACTG 
GATAATGGAG CTGATATGAA TCTAGTGGCT TGTGATCCCA GCAGGTCTAG TGGTGAAAAA 
GATGAGCAGA CATGTTTGAT GTGGGCTTAT GAAAAAGGGC ATGATGCCAT TGTCACACTC 
CTGAAGCATT ATAAGAGACC ACAAGATGAA TTGCCCTGTA ATGAATATTC TCAGCCTGGA 
GGAGATGGCT CCTATGTGTC TGTTCCATCA CCCTTGGGGA AGATTAAAAG CATGACAAAA 
GAGAAGGCAG ATATTCTCCT CCTAAGAGCT GGATTGCCTT CACATTTCCA TCTTCAGCTC 
TCAGAAATTG AGTTCCATGA GATTATTGGC TCAGGTTCTT TTGGGAAAGT ATATAAAGGA 
CGATGCAGAA ATAAAATAGT GGCTATAAAA CGTTATCGAG CCAATACCTA CTGCTCCAAG 
TCAGATGTGG ATATGTTTTG CCGAGAGGTG TCCATTCTCT GCCAGCTCAA TCATCCCTGC 
GTAATTCAGT TTGTGGGTGC TTGCTTGAAT GATCCCAGCC AGTTTGCCAT TGTCACTCAA 
TACATATCAG GGGGTTCTCT GTTCTCCCTC CTTCATGAGC AGAAGAGGAT TCTTGATTTG 
CAGTCTAAAT TAATTATTGC AGTAGATGTT GCCAAAGGCA TGGAGTACCT TCACAACCTG 
ACACAGCCAA TTATACATCG TGACTTGAAC AGTCACAATA TTCTTCTCTA TGAGGATGGG 
CATGCTGTGG TGGCAGATTT TGGAGAATCA AGATTTCTAC AGTCTCTGGA TGAAGACAAC 
ATGACAAAAC AACCTGGGAA CCTCCGTTGG ATGGCTCCTG AGGTGTTCAC GCAGTGCACT 
CGGTACACCA TCAAAGCAGA TGTCTTCAGC TATGCTCTGT GTCTGTGGGA AATTCTCACT 
GGCGAAATTC CATTCGCTCA TCTCAAGCCA GCGGCTGCGG CAGCAGACAT GGCTTACCAC 
CACATCAGAC CTCCCATTGG CTATTCCATT CCCAAGCCCA TATCATCTCT GCTGATACGA 
GGGTGGAACG CATGTCCTGA AGGAAGACCC GAATTTTCTG AAGTTGTCAT GAAGTTAGAA 
GAGTGTCTCT GCAACATTGA GCTGATGTCT CCTGCATCAA GTAACAGCAG TGGGTCTCTC 
TCACCTTCTT CTTCTTCTGA TTGCCTGGTG AACCGGGGAG GACCTGGCCG GAGTCATGTG 
GCAGCATTAA GAAGTCGTTT CGAATTGGAA TATGCTCTAA ATGCAAGGTC CTATGCTGCT 
TTGTCCCAAA GTGCTGGACA ATATTCCTCT CAAGGTCTGT CTTTGGAGGA GATGAAAAGA 
AGTCTTCAAT ACACACCCAT TGACAAATAT GGCTATGTAT CCGATCCCAT GAGCTCAATG 
CATTTTCATT CTTGCCGAAA TAGTAGCAGC TTTGAGGACA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNNI3K ( NM_015978.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057062.1 Homo sapiens TNNI3 interacting kinase (TNNI3K), mRNA.

Price & Availability↑

CloneID	OHu19579
Clone ID Related Accession (Same CDS sequence)	NM_015978.2 , NM_015978.3
Accession Version	NM_015978.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase TNNI3K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX502807.1, BC113539.1 and AA758546.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_015978.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF116826.1, AY303691.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000326637.8/ ENSP00000322251.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGAAATT ATAAATCTAG ACCAACCCAA ACTTGTACTG ATGAATGGAA GAAAAAAGTC 
AGTGAATCAT ATGTTATCAC AATAGAAAGA TTAGAAGATG ACCTGCAGAT CAAGGAAAAA 
GAACTGACAG AACTAAGGAA TATATTTGGC TCTGATGAAG CCTTCAGTAA AGTCAATTTA 
AATTACCGCA CTGAAAATGG GCTGTCTCTA CTTCATTTAT GTTGCATTTG TGGAGGCAAG 
AAATCACATA TTCGAACTCT TATGTTGAAA GGGCTCCGCC CATCTCGACT GACAAGAAAT 
GGATTTACAG CCTTGCATTT AGCAGTTTAC AAGGATAATG CAGAATTGAT CACTTCTCTG 
CTTCACAGTG GAGCTGATAT ACAGCAGGTT GGATACGGTG GCCTCACTGC CCTCCATATT 
GCTACAATAG CTGGCCACCT AGAGGCTGCT GATGTGCTGT TGCAACATGG AGCTAATGTC 
AATATTCAAG ATGCAGTTTT TTTCACTCCA TTGCATATTG CAGCGTACTA TGGACATGAA 
CAGGTAACTC GCCTTCTTTT GAAATTTGGT GCTGATGTAA ATGTAAGTGG TGAAGTTGGA 
GATAGACCCC TCCACCTAGC ATCTGCAAAA GGATTCTTGA ATATTGCAAA ACTCTTGATG 
GAAGAAGGCA GCAAAGCAGA TGTGAATGCT CAAGATAATG AAGACCATGT CCCACTCCAT 
TTCTGTTCTC GATTTGGACA CCATGATATA GTTAAGTATC TGCTGCAAAG TGATTTGGAA 
GTTCAACCTC ATGTTGTTAA TATCTATGGA GATACCCCCT TACACCTGGC ATGCTACAAT 
GGCAAATTTG AAGTTGCCAA GGAAATCATC CAAATATCAG GAACAGAAAG TCTGACTAAG 
GAAAACATCT TCAGTGAAAC AGCTTTTCAT AGTGCTTGTA CCTATGGCAA GAGCATTGAC 
CTAGTCAAAT TTCTTCTTGA TCAGAATGTC ATAAACATCA ACCACCAAGG AAGGGATGGG 
CACACTGGAT TACACTCTGC TTGCTACCAC GGTCACATTC GCCTGGTTCA GTTCTTACTG 
GATAATGGAG CTGATATGAA TCTAGTGGCT TGTGATCCCA GCAGGTCTAG TGGTGAAAAA 
GATGAGCAGA CATGTTTGAT GTGGGCTTAT GAAAAAGGGC ATGATGCCAT TGTCACACTC 
CTGAAGCATT ATAAGAGACC ACAAGATGAA TTGCCCTGTA ATGAATATTC TCAGCCTGGA 
GGAGATGGCT CCTATGTGTC TGTTCCATCA CCCTTGGGGA AGATTAAAAG CATGACAAAA 
GAGAAGGCAG ATATTCTCCT CCTAAGAGCT GGATTGCCTT CACATTTCCA TCTTCAGCTC 
TCAGAAATTG AGTTCCATGA GATTATTGGC TCAGGTTCTT TTGGGAAAGT ATATAAAGGA 
CGATGCAGAA ATAAAATAGT GGCTATAAAA CGTTATCGAG CCAATACCTA CTGCTCCAAG 
TCAGATGTGG ATATGTTTTG CCGAGAGGTG TCCATTCTCT GCCAGCTCAA TCATCCCTGC 
GTAATTCAGT TTGTGGGTGC TTGCTTGAAT GATCCCAGCC AGTTTGCCAT TGTCACTCAA 
TACATATCAG GGGGTTCTCT GTTCTCCCTC CTTCATGAGC AGAAGAGGAT TCTTGATTTG 
CAGTCTAAAT TAATTATTGC AGTAGATGTT GCCAAAGGCA TGGAGTACCT TCACAACCTG 
ACACAGCCAA TTATACATCG TGACTTGAAC AGTCACAATA TTCTTCTCTA TGAGGATGGG 
CATGCTGTGG TGGCAGATTT TGGAGAATCA AGATTTCTAC AGTCTCTGGA TGAAGACAAC 
ATGACAAAAC AACCTGGGAA CCTCCGTTGG ATGGCTCCTG AGGTGTTCAC GCAGTGCACT 
CGGTACACCA TCAAAGCAGA TGTCTTCAGC TATGCTCTGT GTCTGTGGGA AATTCTCACT 
GGCGAAATTC CATTCGCTCA TCTCAAGCCA GCGGCTGCGG CAGCAGACAT GGCTTACCAC 
CACATCAGAC CTCCCATTGG CTATTCCATT CCCAAGCCCA TATCATCTCT GCTGATACGA 
GGGTGGAACG CATGTCCTGA AGGAAGACCC GAATTTTCTG AAGTTGTCAT GAAGTTAGAA 
GAGTGTCTCT GCAACATTGA GCTGATGTCT CCTGCATCAA GTAACAGCAG TGGGTCTCTC 
TCACCTTCTT CTTCTTCTGA TTGCCTGGTG AACCGGGGAG GACCTGGCCG GAGTCATGTG 
GCAGCATTAA GAAGTCGTTT CGAATTGGAA TATGCTCTAA ATGCAAGGTC CTATGCTGCT 
TTGTCCCAAA GTGCTGGACA ATATTCCTCT CAAGGTCTGT CTTTGGAGGA GATGAAAAGA 
AGTCTTCAAT ACACACCCAT TGACAAATAT GGCTATGTAT CCGATCCCAT GAGCTCAATG 
CATTTTCATT CTTGCCGAAA TAGTAGCAGC TTTGAGGACA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057062.1
CDS	66..2573
Translation	MGNYKSRPTQTCTDEWKKKVSESYVITIERLEDDLQIKEKELTELRNIFGSDEAFSKVNLNYRTENGLSLLHLCCICGGKKSHIRTLMLKGLRPSRLTRNGFTALHLAVYKDNAELITSLLHSGADIQQVGYGGLTALHIATIAGHLEAADVLLQHGANVNIQDAVFFTPLHIAAYYGHEQVTRLLLKFGADVNVSGEVGDRPLHLASAKGFLNIAKLLMEEGSKADVNAQDNEDHVPLHFCSRFGHHDIVKYLLQSDLEVQPHVVNIYGDTPLHLACYNGKFEVAKEIIQISGTESLTKENIFSETAFHSACTYGKSIDLVKFLLDQNVININHQGRDGHTGLHSACYHGHIRLVQFLLDNGADMNLVACDPSRSSGEKDEQTCLMWAYEKGHDAIVTLLKHYKRPQDELPCNEYSQPGGDGSYVSVPSPLGKIKSMTKEKADILLLRAGLPSHFHLQLSEIEFHEIIGSGSFGKVYKGRCRNKIVAIKRYRANTYCSKSDVDMFCREVSILCQLNHPCVIQFVGACLNDPSQFAIVTQYISGGSLFSLLHEQKRILDLQSKLIIAVDVAKGMEYLHNLTQPIIHRDLNSHNILLYEDGHAVVADFGESRFLQSLDEDNMTKQPGNLRWMAPEVFTQCTRYTIKADVFSYALCLWEILTGEIPFAHLKPAAAAADMAYHHIRPPIGYSIPKPISSLLIRGWNACPEGRPEFSEVVMKLEECLCNIELMSPASSNSSGSLSPSSSSDCLVNRGGPGRSHVAALRSRFELEYALNARSYAALSQSAGQYSSQGLSLEEMKRSLQYTPIDKYGYVSDPMSSMHFHSCRNSSSFEDSS

Target ORF information:

RefSeq Version	NM_015978.3
Organism	Homo sapiens(human)
Definition	Homo sapiens TNNI3 interacting kinase (TNNI3K), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015978.3

ORF Insert Sequence:

ATGGGAAATT ATAAATCTAG ACCAACCCAA ACTTGTACTG ATGAATGGAA GAAAAAAGTC 
AGTGAATCAT ATGTTATCAC AATAGAAAGA TTAGAAGATG ACCTGCAGAT CAAGGAAAAA 
GAACTGACAG AACTAAGGAA TATATTTGGC TCTGATGAAG CCTTCAGTAA AGTCAATTTA 
AATTACCGCA CTGAAAATGG GCTGTCTCTA CTTCATTTAT GTTGCATTTG TGGAGGCAAG 
AAATCACATA TTCGAACTCT TATGTTGAAA GGGCTCCGCC CATCTCGACT GACAAGAAAT 
GGATTTACAG CCTTGCATTT AGCAGTTTAC AAGGATAATG CAGAATTGAT CACTTCTCTG 
CTTCACAGTG GAGCTGATAT ACAGCAGGTT GGATACGGTG GCCTCACTGC CCTCCATATT 
GCTACAATAG CTGGCCACCT AGAGGCTGCT GATGTGCTGT TGCAACATGG AGCTAATGTC 
AATATTCAAG ATGCAGTTTT TTTCACTCCA TTGCATATTG CAGCGTACTA TGGACATGAA 
CAGGTAACTC GCCTTCTTTT GAAATTTGGT GCTGATGTAA ATGTAAGTGG TGAAGTTGGA 
GATAGACCCC TCCACCTAGC ATCTGCAAAA GGATTCTTGA ATATTGCAAA ACTCTTGATG 
GAAGAAGGCA GCAAAGCAGA TGTGAATGCT CAAGATAATG AAGACCATGT CCCACTCCAT 
TTCTGTTCTC GATTTGGACA CCATGATATA GTTAAGTATC TGCTGCAAAG TGATTTGGAA 
GTTCAACCTC ATGTTGTTAA TATCTATGGA GATACCCCCT TACACCTGGC ATGCTACAAT 
GGCAAATTTG AAGTTGCCAA GGAAATCATC CAAATATCAG GAACAGAAAG TCTGACTAAG 
GAAAACATCT TCAGTGAAAC AGCTTTTCAT AGTGCTTGTA CCTATGGCAA GAGCATTGAC 
CTAGTCAAAT TTCTTCTTGA TCAGAATGTC ATAAACATCA ACCACCAAGG AAGGGATGGG 
CACACTGGAT TACACTCTGC TTGCTACCAC GGTCACATTC GCCTGGTTCA GTTCTTACTG 
GATAATGGAG CTGATATGAA TCTAGTGGCT TGTGATCCCA GCAGGTCTAG TGGTGAAAAA 
GATGAGCAGA CATGTTTGAT GTGGGCTTAT GAAAAAGGGC ATGATGCCAT TGTCACACTC 
CTGAAGCATT ATAAGAGACC ACAAGATGAA TTGCCCTGTA ATGAATATTC TCAGCCTGGA 
GGAGATGGCT CCTATGTGTC TGTTCCATCA CCCTTGGGGA AGATTAAAAG CATGACAAAA 
GAGAAGGCAG ATATTCTCCT CCTAAGAGCT GGATTGCCTT CACATTTCCA TCTTCAGCTC 
TCAGAAATTG AGTTCCATGA GATTATTGGC TCAGGTTCTT TTGGGAAAGT ATATAAAGGA 
CGATGCAGAA ATAAAATAGT GGCTATAAAA CGTTATCGAG CCAATACCTA CTGCTCCAAG 
TCAGATGTGG ATATGTTTTG CCGAGAGGTG TCCATTCTCT GCCAGCTCAA TCATCCCTGC 
GTAATTCAGT TTGTGGGTGC TTGCTTGAAT GATCCCAGCC AGTTTGCCAT TGTCACTCAA 
TACATATCAG GGGGTTCTCT GTTCTCCCTC CTTCATGAGC AGAAGAGGAT TCTTGATTTG 
CAGTCTAAAT TAATTATTGC AGTAGATGTT GCCAAAGGCA TGGAGTACCT TCACAACCTG 
ACACAGCCAA TTATACATCG TGACTTGAAC AGTCACAATA TTCTTCTCTA TGAGGATGGG 
CATGCTGTGG TGGCAGATTT TGGAGAATCA AGATTTCTAC AGTCTCTGGA TGAAGACAAC 
ATGACAAAAC AACCTGGGAA CCTCCGTTGG ATGGCTCCTG AGGTGTTCAC GCAGTGCACT 
CGGTACACCA TCAAAGCAGA TGTCTTCAGC TATGCTCTGT GTCTGTGGGA AATTCTCACT 
GGCGAAATTC CATTCGCTCA TCTCAAGCCA GCGGCTGCGG CAGCAGACAT GGCTTACCAC 
CACATCAGAC CTCCCATTGG CTATTCCATT CCCAAGCCCA TATCATCTCT GCTGATACGA 
GGGTGGAACG CATGTCCTGA AGGAAGACCC GAATTTTCTG AAGTTGTCAT GAAGTTAGAA 
GAGTGTCTCT GCAACATTGA GCTGATGTCT CCTGCATCAA GTAACAGCAG TGGGTCTCTC 
TCACCTTCTT CTTCTTCTGA TTGCCTGGTG AACCGGGGAG GACCTGGCCG GAGTCATGTG 
GCAGCATTAA GAAGTCGTTT CGAATTGGAA TATGCTCTAA ATGCAAGGTC CTATGCTGCT 
TTGTCCCAAA GTGCTGGACA ATATTCCTCT CAAGGTCTGT CTTTGGAGGA GATGAAAAGA 
AGTCTTCAAT ACACACCCAT TGACAAATAT GGCTATGTAT CCGATCCCAT GAGCTCAATG 
CATTTTCATT CTTGCCGAAA TAGTAGCAGC TTTGAGGACA GCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Whole exome sequencing identifies a novel mutation (c.333?+?2T?>?C) of TNNI3K in a Chinese family with dilated cardiomyopathy and cardiac conduction disease.
Gene(2018 Jan)
Fan LL,Huang H,Jin JY,Li JJ,Chen YQ,Zhao SP,Xiang R

ACE2, CALM3 and TNNI3K polymorphisms as potential disease modifiers in hypertrophic and dilated cardiomyopathies.
Molecular and cellular biochemistry438(1-2)167-174(2018 Jan)
Kumar A,Rani B,Sharma R,Kaur G,Prasad R,Bahl A,Khullar M

Overexpression of Cardiac-Specific Kinase TNNI3K Promotes Mouse Embryonic Stem Cells Differentiation into Cardiomyocytes.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology41(1)381-398(2017)
Wang Y,Wang SQ,Wang LP,Yao YH,Ma CY,Ding JF,Ye J,Meng XM,Li JJ,Xu RX

Parent-of-origin-specific allelic associations among 106 genomic loci for age at menarche.
Nature514(7520)92-97(2014 Oct)
Perry JR,Day F,Elks CE,Sulem P,Thompson DJ,Ferreira T,He C,Chasman DI,Esko T,Thorleifsson G,Albrecht E,Ang WQ,Corre T,Cousminer DL,Feenstra B,Franceschini N,Ganna A,Johnson AD,Kjellqvist S,Lunetta KL,McMahon G,Nolte IM,Paternoster L,Porcu E,Smith AV,Stolk L,Teumer A,T?ernikova N,Tikkanen E,Ulivi S,Wagner EK,Amin N,Bierut LJ,Byrne EM,Hottenga JJ,Koller DL,Mangino M,Pers TH,Yerges-Armstrong LM,Zhao JH,Andrulis IL,Anton-Culver H,Atsma F,Bandinelli S,Beckmann MW,Benitez J,Blomqvist C,Bojesen SE,Bolla MK,Bonanni B,Brauch H,Brenner H,Buring JE,Chang-Claude J,Chanock S,Chen J,Chenevix-Trench G,Coll?e JM,Couch FJ,Couper D,Coveillo AD,Cox A,Czene K,D'adamo AP,Smith GD,De Vivo I,Demerath EW,Dennis J,Devilee P,Dieffenbach AK,Dunning AM,Eiriksdottir G,Eriksson JG,Fasching PA,Ferrucci L,Flesch-Janys D,Flyger H,Foroud T,Franke L,Garcia ME,Garc?a-Closas M,Geller F,de Geus EE,Giles GG,Gudbjartsson DF,Gudnason V,Gu?nel P,Guo S,Hall P,Hamann U,Haring R,Hartman CA,Heath AC,Hofman A,Hooning MJ,Hopper JL,Hu FB,Hunter DJ,Karasik D,Kiel DP,Knight JA,Kosma VM,Kutalik Z,Lai S,Lambrechts D,Lindblom A,M?gi R,Magnusson PK,Mannermaa A,Martin NG,Masson G,McArdle PF,McArdle WL,Melbye M,Michailidou K,Mihailov E,Milani L,Milne RL,Nevanlinna H,Neven P,Nohr EA,Oldehinkel AJ,Oostra BA,Palotie A,Peacock M,Pedersen NL,Peterlongo P,Peto J,Pharoah PD,Postma DS,Pouta A,Pylk?s K,Radice P,Ring S,Rivadeneira F,Robino A,Rose LM,Rudolph A,Salomaa V,Sanna S,Schlessinger D,Schmidt MK,Southey MC,Sovio U,Stampfer MJ,St?ckl D,Storniolo AM,Timpson NJ,Tyrer J,Visser JA,Vollenweider P,V?lzke H,Waeber G,Waldenberger M,Wallaschofski H,Wang Q,Willemsen G,Winqvist R,Wolffenbuttel BH,Wright MJ,,,,,,,Boomsma DI,Econs MJ,Khaw KT,Loos RJ,McCarthy MI,Montgomery GW,Rice JP,Streeten EA,Thorsteinsdottir U,van Duijn CM,Alizadeh BZ,Bergmann S,Boerwinkle E,Boyd HA,Crisponi L,Gasparini P,Gieger C,Harris TB,Ingelsson E,J?rvelin MR,Kraft P,Lawlor D,Metspalu A,Pennell CE,Ridker PM,Snieder H,S?rensen TI,Spector TD,Strachan DP,Uitterlinden AG,Wareham NJ,Widen E,Zygmunt M,Murray A,Easton DF,Stefansson K,Murabito JM,Ong KK

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TNNI3K cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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