DERL2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DERL2
Entrez Gene ID	51009
Full Name	derlin 2
Synonyms	CGI-101,DERtrin-2,F-LAN-1,F-LANa,FLANa,derlin-2
General protein information	Preferred Names derlin 2 Names derlin-2 Der1-like domain family, member 2 carcinoma related degradation in endoplasmic reticulum protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.2
Summary	Proteins that are unfolded or misfolded in the endoplasmic reticulum (ER) must be refolded or degraded to maintain the homeostasis of the ER. DERL2 is involved in the degradation of misfolded glycoproteins in the ER (Oda et al., 2006 [PubMed 16449189]).[supplied by OMIM, Mar 2008].
Disorder MIM:	610304

mRNA and Protein(s)

mRNA	Protein	Name
NM_001304779.1	NP_001291708.1	derlin-2 isoform c
NM_001304777.1	NP_001291706.1	derlin-2 isoform b
NM_001304779.2	NP_001291708.1	derlin-2 isoform c
NM_016041.5	NP_057125.2	derlin-2 isoform a
NM_001304777.2	NP_001291706.1	derlin-2 isoform b
NM_016041.4	NP_057125.2	derlin-2 isoform a

Pathways from BioSystems

Homologies

	DERL2	XP_001101490.1
Bos taurus (cattle)	DERL2	NP_001030231.2
Magnaporthe oryzae (rice blast fungus)	MGG_06444	XP_003717123.1
Pan troglodytes (chimpanzee)	DERL2	XP_511992.3
Caenorhabditis elegans (roundworm)	R151.6	NP_498590.4
Homo sapiens (human)	DERL2	NP_057125.2
Arabidopsis thaliana (thale cress)	DER2.2	NP_192395.1
Canis lupus familiaris (dog)	DERL2	XP_848354.1
Mus musculus (house mouse)	Derl2	NP_291040.1
Rattus norvegicus (Norway rat)	LOC100910823	XP_006246938.1
Drosophila melanogaster (fruit fly)	Der-2	NP_650553.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000832	XP_316798.3
Neurospora crassa	NCU00146	XP_956659.1
Arabidopsis thaliana (thale cress)	DER2.1	NP_193912.3
Xenopus tropicalis (tropical clawed frog)	derl2	NP_001016097.1
Danio rerio (zebrafish)	derl2	NP_001019566.1
Oryza sativa (rice)	Os03g0852200	NP_001051921.1

Gene Ontology
Bibliography

Related articles in PubMed

Podocytes Exhibit a Specialized Protein Quality Control Employing Derlin-2 in Kidney Disease.
Ren G, Tardi NJ, Matsuda F, Koh KH, Ruiz P, Wei C, Altintas MM, Ploegh H, Reiser J
American journal of physiology. Renal physiologyajprenal006912016(2017 Nov)

Pre-emptive Quality Control Protects the ER from Protein Overload via the Proximity of ERAD Components and SRP.
Kadowaki H, Nagai A, Maruyama T, Takami Y, Satrimafitrah P, Kato H, Honda A, Hatta T, Natsume T, Sato T, Kai H, Ichijo H, Nishitoh H
Cell reports13(5)944-56(2015 Nov)

Identification of ERAD components essential for dislocation of the null Hong Kong variant of ?-1-antitrypsin (NHK).
Zhong Y, Shen H, Wang Y, Yang Y, Yang P, Fang S
Biochemical and biophysical research communications458(2)424-8(2015 Mar)

Proteasomal Degradation of Proinsulin Requires Derlin-2, HRD1 and p97.
Hoelen H, Zaldumbide A, van Leeuwen WF, Torfs EC, Engelse MA, Hassan C, Lebbink RJ, de Koning EJ, Resssing ME, de Ru AH, van Veelen PA, Hoeben RC, Roep BO, Wiertz EJ
PloS one10(6)e0128206(2015)

Derlin2 protein facilitates HRD1-mediated retro-translocation of sonic hedgehog at the endoplasmic reticulum.
Huang CH, Hsiao HT, Chu YR, Ye Y, Chen X
The Journal of biological chemistry288(35)25330-9(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

specific silencing of Derlin-2, p97 and HRD1 by shRNAs increases steady state levels of proinsulin. these ERAD constituents are critically involved in proinsulin degradation and may therefore also play a role in subsequent antigen generation.
Title: Proteasomal Degradation of Proinsulin Requires Derlin-2, HRD1 and p97.

derlin2 functions with HRD1 in ERAD of certain substrates independent of their glycosylation status.
Title: Derlin2 protein facilitates HRD1-mediated retro-translocation of sonic hedgehog at the endoplasmic reticulum.

Required for Polyoma virus infection.
Title: Murine polyomavirus requires the endoplasmic reticulum protein Derlin-2 to initiate infection.

Findings indicate that Derlin-2 provides the missing link between EDEM and p97 in the process of degrading misfolded glycoproteins.
Title: Derlin-2 and Derlin-3 are regulated by the mammalian unfolded protein response and are required for ER-associated degradation.

Derlin-2 forms a robust multiprotein complex with the p97 AAA ATPase as well as the mammalian orthologs of the yeast Hrd1p/Hrd3p ubiquitin-ligase complex, and participates in the degradation of proteins from the ER.
Title: Multiprotein complexes that link dislocation, ubiquitination, and extraction of misfolded proteins from the endoplasmic reticulum membrane.

The following DERL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DERL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07161	NM_016041.5 Latest version!	Homo sapiens derlin 2 (DERL2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu49863	NM_001304777.2 Latest version!	Homo sapiens derlin 2 (DERL2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu67576	NM_001304779.2 Latest version!	Homo sapiens derlin 2 (DERL2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu67576	NM_001304779.1	Homo sapiens derlin 2 (DERL2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu49863	NM_001304777.1	Homo sapiens derlin 2 (DERL2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu07161	NM_016041.4	Homo sapiens derlin 2 (DERL2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DERL2 ( NM_016041.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057125.2 Homo sapiens derlin 2 (DERL2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu07161
Clone ID Related Accession (Same CDS sequence)	NM_016041.5 , NM_016041.4
Accession Version	NM_016041.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	derlin-2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY051246.1, AF242523.1, AC004148.1 and BM675359.1. On Nov 23, 2018 this sequence version replaced NM_016041.4. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.219624.1, SRR1803613.182735.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000158771.9/ ENSP00000158771.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGTACC AGAGCTTGCG GCTGGAGTAC CTGCAGATCC CACCGGTCAG CCGCGCCTAC 
ACCACTGCCT GCGTCCTCAC CACCGCCGCC GTGCAGTTGG AATTGATCAC ACCTTTTCAG 
TTGTACTTCA ATCCTGAATT AATCTTTAAA CACTTTCAAA TATGGAGATT AATCACCAAC 
TTCTTATTTT TTGGGCCAGT TGGATTCAAT TTTTTATTTA ACATGATTTT TCTATATCGT 
TACTGTCGAA TGCTAGAAGA AGGCTCTTTC CGAGGTCGGA CAGCAGACTT TGTATTTATG 
TTCCTTTTTG GTGGATTCTT AATGACCCTT TTTGGTCTGT TTGTGAGCTT AGTTTTCTTG 
GGCCAGGCCT TTACAATAAT GCTCGTCTAT GTGTGGAGCC GAAGGAACCC CTATGTCCGC 
ATGAACTTCT TCGGCCTTCT CAACTTCCAG GCCCCCTTTC TGCCCTGGGT GCTCATGGGA 
TTTTCCTTGT TGTTGGGGAA CTCAATCATT GTGGACCTTT TGGGTATTGC AGTTGGACAC 
ATATATTTTT TCTTGGAAGA TGTATTTCCC AATCAACCTG GTGGAATAAG AATTCTGAAA 
ACACCATCTA TTTTGAAAGC TATTTTTGAT ACACCAGATG AGGATCCAAA TTACAATCCA 
CTACCTGAGG AACGGCCAGG AGGCTTCGCC TGGGGTGAGG GCCAGCGGCT TGGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057125.2
CDS	18..737
Translation	MAYQSLRLEYLQIPPVSRAYTTACVLTTAAVQLELITPFQLYFNPELIFKHFQIWRLITNFLFFGPVGFNFLFNMIFLYRYCRMLEEGSFRGRTADFVFMFLFGGFLMTLFGLFVSLVFLGQAFTIMLVYVWSRRNPYVRMNFFGLLNFQAPFLPWVLMGFSLLLGNSIIVDLLGIAVGHIYFFLEDVFPNQPGGIRILKTPSILKAIFDTPDEDPNYNPLPEERPGGFAWGEGQRLGG

Target ORF information:

RefSeq Version	NM_016041.5
Organism	Homo sapiens(human)
Definition	Homo sapiens derlin 2 (DERL2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016041.5

ORF Insert Sequence:

ATGGCGTACC AGAGCTTGCG GCTGGAGTAC CTGCAGATCC CACCGGTCAG CCGCGCCTAC 
ACCACTGCCT GCGTCCTCAC CACCGCCGCC GTGCAGTTGG AATTGATCAC ACCTTTTCAG 
TTGTACTTCA ATCCTGAATT AATCTTTAAA CACTTTCAAA TATGGAGATT AATCACCAAC 
TTCTTATTTT TTGGGCCAGT TGGATTCAAT TTTTTATTTA ACATGATTTT TCTATATCGT 
TACTGTCGAA TGCTAGAAGA AGGCTCTTTC CGAGGTCGGA CAGCAGACTT TGTATTTATG 
TTCCTTTTTG GTGGATTCTT AATGACCCTT TTTGGTCTGT TTGTGAGCTT AGTTTTCTTG 
GGCCAGGCCT TTACAATAAT GCTCGTCTAT GTGTGGAGCC GAAGGAACCC CTATGTCCGC 
ATGAACTTCT TCGGCCTTCT CAACTTCCAG GCCCCCTTTC TGCCCTGGGT GCTCATGGGA 
TTTTCCTTGT TGTTGGGGAA CTCAATCATT GTGGACCTTT TGGGTATTGC AGTTGGACAC 
ATATATTTTT TCTTGGAAGA TGTATTTCCC AATCAACCTG GTGGAATAAG AATTCTGAAA 
ACACCATCTA TTTTGAAAGC TATTTTTGAT ACACCAGATG AGGATCCAAA TTACAATCCA 
CTACCTGAGG AACGGCCAGG AGGCTTCGCC TGGGGTGAGG GCCAGCGGCT TGGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DERL2 ( NM_016041.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057125.2 Homo sapiens derlin 2 (DERL2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu07161
Clone ID Related Accession (Same CDS sequence)	NM_016041.5 , NM_016041.4
Accession Version	NM_016041.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	derlin-2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY051246.1, AF242523.1, AC004148.1 and BM675359.1. On Feb 10, 2015 this sequence version replaced NM_016041.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.182735.1, SRR1660807.92548.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTACC AGAGCTTGCG GCTGGAGTAC CTGCAGATCC CACCGGTCAG CCGCGCCTAC 
ACCACTGCCT GCGTCCTCAC CACCGCCGCC GTGCAGTTGG AATTGATCAC ACCTTTTCAG 
TTGTACTTCA ATCCTGAATT AATCTTTAAA CACTTTCAAA TATGGAGATT AATCACCAAC 
TTCTTATTTT TTGGGCCAGT TGGATTCAAT TTTTTATTTA ACATGATTTT TCTATATCGT 
TACTGTCGAA TGCTAGAAGA AGGCTCTTTC CGAGGTCGGA CAGCAGACTT TGTATTTATG 
TTCCTTTTTG GTGGATTCTT AATGACCCTT TTTGGTCTGT TTGTGAGCTT AGTTTTCTTG 
GGCCAGGCCT TTACAATAAT GCTCGTCTAT GTGTGGAGCC GAAGGAACCC CTATGTCCGC 
ATGAACTTCT TCGGCCTTCT CAACTTCCAG GCCCCCTTTC TGCCCTGGGT GCTCATGGGA 
TTTTCCTTGT TGTTGGGGAA CTCAATCATT GTGGACCTTT TGGGTATTGC AGTTGGACAC 
ATATATTTTT TCTTGGAAGA TGTATTTCCC AATCAACCTG GTGGAATAAG AATTCTGAAA 
ACACCATCTA TTTTGAAAGC TATTTTTGAT ACACCAGATG AGGATCCAAA TTACAATCCA 
CTACCTGAGG AACGGCCAGG AGGCTTCGCC TGGGGTGAGG GCCAGCGGCT TGGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057125.2
CDS	70..789
Translation	MAYQSLRLEYLQIPPVSRAYTTACVLTTAAVQLELITPFQLYFNPELIFKHFQIWRLITNFLFFGPVGFNFLFNMIFLYRYCRMLEEGSFRGRTADFVFMFLFGGFLMTLFGLFVSLVFLGQAFTIMLVYVWSRRNPYVRMNFFGLLNFQAPFLPWVLMGFSLLLGNSIIVDLLGIAVGHIYFFLEDVFPNQPGGIRILKTPSILKAIFDTPDEDPNYNPLPEERPGGFAWGEGQRLGG

Target ORF information:

RefSeq Version	NM_016041.4
Organism	Homo sapiens(human)
Definition	Homo sapiens derlin 2 (DERL2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016041.4

ORF Insert Sequence:

ATGGCGTACC AGAGCTTGCG GCTGGAGTAC CTGCAGATCC CACCGGTCAG CCGCGCCTAC 
ACCACTGCCT GCGTCCTCAC CACCGCCGCC GTGCAGTTGG AATTGATCAC ACCTTTTCAG 
TTGTACTTCA ATCCTGAATT AATCTTTAAA CACTTTCAAA TATGGAGATT AATCACCAAC 
TTCTTATTTT TTGGGCCAGT TGGATTCAAT TTTTTATTTA ACATGATTTT TCTATATCGT 
TACTGTCGAA TGCTAGAAGA AGGCTCTTTC CGAGGTCGGA CAGCAGACTT TGTATTTATG 
TTCCTTTTTG GTGGATTCTT AATGACCCTT TTTGGTCTGT TTGTGAGCTT AGTTTTCTTG 
GGCCAGGCCT TTACAATAAT GCTCGTCTAT GTGTGGAGCC GAAGGAACCC CTATGTCCGC 
ATGAACTTCT TCGGCCTTCT CAACTTCCAG GCCCCCTTTC TGCCCTGGGT GCTCATGGGA 
TTTTCCTTGT TGTTGGGGAA CTCAATCATT GTGGACCTTT TGGGTATTGC AGTTGGACAC 
ATATATTTTT TCTTGGAAGA TGTATTTCCC AATCAACCTG GTGGAATAAG AATTCTGAAA 
ACACCATCTA TTTTGAAAGC TATTTTTGAT ACACCAGATG AGGATCCAAA TTACAATCCA 
CTACCTGAGG AACGGCCAGG AGGCTTCGCC TGGGGTGAGG GCCAGCGGCT TGGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DERL2 ( NM_001304777.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001291706.1 Homo sapiens derlin 2 (DERL2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu49863
Clone ID Related Accession (Same CDS sequence)	NM_001304777.1 , NM_001304777.2
Accession Version	NM_001304777.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	derlin-2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY051246.1, CN362881.1, AC004148.1 and BM675359.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is 1 aa shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.135195.1, SRR1803614.224498.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTACC AGAGCTTGCG GCTGGAGTAC CTGCAGATCC CACCGGTCAG CCGCGCCTAC 
ACCACTGCCT GCGTCCTCAC CACCGCCGCC GTGTTGGAAT TGATCACACC TTTTCAGTTG 
TACTTCAATC CTGAATTAAT CTTTAAACAC TTTCAAATAT GGAGATTAAT CACCAACTTC 
TTATTTTTTG GGCCAGTTGG ATTCAATTTT TTATTTAACA TGATTTTTCT ATATCGTTAC 
TGTCGAATGC TAGAAGAAGG CTCTTTCCGA GGTCGGACAG CAGACTTTGT ATTTATGTTC 
CTTTTTGGTG GATTCTTAAT GACCCTTTTT GGTCTGTTTG TGAGCTTAGT TTTCTTGGGC 
CAGGCCTTTA CAATAATGCT CGTCTATGTG TGGAGCCGAA GGAACCCCTA TGTCCGCATG 
AACTTCTTCG GCCTTCTCAA CTTCCAGGCC CCCTTTCTGC CCTGGGTGCT CATGGGATTT 
TCCTTGTTGT TGGGGAACTC AATCATTGTG GACCTTTTGG GTATTGCAGT TGGACACATA 
TATTTTTTCT TGGAAGATGT ATTTCCCAAT CAACCTGGTG GAATAAGAAT TCTGAAAACA 
CCATCTATTT TGAAAGCTAT TTTTGATACA CCAGATGAGG ATCCAAATTA CAATCCACTA 
CCTGAGGAAC GGCCAGGAGG CTTCGCCTGG GGTGAGGGCC AGCGGCTTGG AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291706.1
CDS	70..786
Translation	MAYQSLRLEYLQIPPVSRAYTTACVLTTAAVLELITPFQLYFNPELIFKHFQIWRLITNFLFFGPVGFNFLFNMIFLYRYCRMLEEGSFRGRTADFVFMFLFGGFLMTLFGLFVSLVFLGQAFTIMLVYVWSRRNPYVRMNFFGLLNFQAPFLPWVLMGFSLLLGNSIIVDLLGIAVGHIYFFLEDVFPNQPGGIRILKTPSILKAIFDTPDEDPNYNPLPEERPGGFAWGEGQRLGG

Target ORF information:

RefSeq Version	NM_001304777.1
Organism	Homo sapiens(human)
Definition	Homo sapiens derlin 2 (DERL2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304777.1

ORF Insert Sequence:

ATGGCGTACC AGAGCTTGCG GCTGGAGTAC CTGCAGATCC CACCGGTCAG CCGCGCCTAC 
ACCACTGCCT GCGTCCTCAC CACCGCCGCC GTGTTGGAAT TGATCACACC TTTTCAGTTG 
TACTTCAATC CTGAATTAAT CTTTAAACAC TTTCAAATAT GGAGATTAAT CACCAACTTC 
TTATTTTTTG GGCCAGTTGG ATTCAATTTT TTATTTAACA TGATTTTTCT ATATCGTTAC 
TGTCGAATGC TAGAAGAAGG CTCTTTCCGA GGTCGGACAG CAGACTTTGT ATTTATGTTC 
CTTTTTGGTG GATTCTTAAT GACCCTTTTT GGTCTGTTTG TGAGCTTAGT TTTCTTGGGC 
CAGGCCTTTA CAATAATGCT CGTCTATGTG TGGAGCCGAA GGAACCCCTA TGTCCGCATG 
AACTTCTTCG GCCTTCTCAA CTTCCAGGCC CCCTTTCTGC CCTGGGTGCT CATGGGATTT 
TCCTTGTTGT TGGGGAACTC AATCATTGTG GACCTTTTGG GTATTGCAGT TGGACACATA 
TATTTTTTCT TGGAAGATGT ATTTCCCAAT CAACCTGGTG GAATAAGAAT TCTGAAAACA 
CCATCTATTT TGAAAGCTAT TTTTGATACA CCAGATGAGG ATCCAAATTA CAATCCACTA 
CCTGAGGAAC GGCCAGGAGG CTTCGCCTGG GGTGAGGGCC AGCGGCTTGG AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DERL2 ( NM_001304777.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001291706.1 Homo sapiens derlin 2 (DERL2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu49863
Clone ID Related Accession (Same CDS sequence)	NM_001304777.1 , NM_001304777.2
Accession Version	NM_001304777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	derlin-2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY051246.1, CN362881.1, AC004148.1 and BM675359.1. On May 31, 2019 this sequence version replaced NM_001304777.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is 1 aa shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.224498.1, SRR1660809.135195.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTACC AGAGCTTGCG GCTGGAGTAC CTGCAGATCC CACCGGTCAG CCGCGCCTAC 
ACCACTGCCT GCGTCCTCAC CACCGCCGCC GTGTTGGAAT TGATCACACC TTTTCAGTTG 
TACTTCAATC CTGAATTAAT CTTTAAACAC TTTCAAATAT GGAGATTAAT CACCAACTTC 
TTATTTTTTG GGCCAGTTGG ATTCAATTTT TTATTTAACA TGATTTTTCT ATATCGTTAC 
TGTCGAATGC TAGAAGAAGG CTCTTTCCGA GGTCGGACAG CAGACTTTGT ATTTATGTTC 
CTTTTTGGTG GATTCTTAAT GACCCTTTTT GGTCTGTTTG TGAGCTTAGT TTTCTTGGGC 
CAGGCCTTTA CAATAATGCT CGTCTATGTG TGGAGCCGAA GGAACCCCTA TGTCCGCATG 
AACTTCTTCG GCCTTCTCAA CTTCCAGGCC CCCTTTCTGC CCTGGGTGCT CATGGGATTT 
TCCTTGTTGT TGGGGAACTC AATCATTGTG GACCTTTTGG GTATTGCAGT TGGACACATA 
TATTTTTTCT TGGAAGATGT ATTTCCCAAT CAACCTGGTG GAATAAGAAT TCTGAAAACA 
CCATCTATTT TGAAAGCTAT TTTTGATACA CCAGATGAGG ATCCAAATTA CAATCCACTA 
CCTGAGGAAC GGCCAGGAGG CTTCGCCTGG GGTGAGGGCC AGCGGCTTGG AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291706.1
CDS	18..734
Translation	MAYQSLRLEYLQIPPVSRAYTTACVLTTAAVLELITPFQLYFNPELIFKHFQIWRLITNFLFFGPVGFNFLFNMIFLYRYCRMLEEGSFRGRTADFVFMFLFGGFLMTLFGLFVSLVFLGQAFTIMLVYVWSRRNPYVRMNFFGLLNFQAPFLPWVLMGFSLLLGNSIIVDLLGIAVGHIYFFLEDVFPNQPGGIRILKTPSILKAIFDTPDEDPNYNPLPEERPGGFAWGEGQRLGG

Target ORF information:

RefSeq Version	NM_001304777.2
Organism	Homo sapiens(human)
Definition	Homo sapiens derlin 2 (DERL2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304777.2

ORF Insert Sequence:

ATGGCGTACC AGAGCTTGCG GCTGGAGTAC CTGCAGATCC CACCGGTCAG CCGCGCCTAC 
ACCACTGCCT GCGTCCTCAC CACCGCCGCC GTGTTGGAAT TGATCACACC TTTTCAGTTG 
TACTTCAATC CTGAATTAAT CTTTAAACAC TTTCAAATAT GGAGATTAAT CACCAACTTC 
TTATTTTTTG GGCCAGTTGG ATTCAATTTT TTATTTAACA TGATTTTTCT ATATCGTTAC 
TGTCGAATGC TAGAAGAAGG CTCTTTCCGA GGTCGGACAG CAGACTTTGT ATTTATGTTC 
CTTTTTGGTG GATTCTTAAT GACCCTTTTT GGTCTGTTTG TGAGCTTAGT TTTCTTGGGC 
CAGGCCTTTA CAATAATGCT CGTCTATGTG TGGAGCCGAA GGAACCCCTA TGTCCGCATG 
AACTTCTTCG GCCTTCTCAA CTTCCAGGCC CCCTTTCTGC CCTGGGTGCT CATGGGATTT 
TCCTTGTTGT TGGGGAACTC AATCATTGTG GACCTTTTGG GTATTGCAGT TGGACACATA 
TATTTTTTCT TGGAAGATGT ATTTCCCAAT CAACCTGGTG GAATAAGAAT TCTGAAAACA 
CCATCTATTT TGAAAGCTAT TTTTGATACA CCAGATGAGG ATCCAAATTA CAATCCACTA 
CCTGAGGAAC GGCCAGGAGG CTTCGCCTGG GGTGAGGGCC AGCGGCTTGG AGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DERL2 ( NM_001304779.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001291708.1 Homo sapiens derlin 2 (DERL2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu67576
Clone ID Related Accession (Same CDS sequence)	NM_001304779.1 , NM_001304779.2
Accession Version	NM_001304779.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	derlin-2 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY051246.1, AF242523.1, BU199049.1, AC004148.1 and BM675359.1. Transcript Variant: This variant (3) lacks three alternate exons in the central coding region, resulting in a frameshift and a shorter 3' coding region, compared to variant 1. The encoded isoform (c) has a distinct C-terminus and is significantly shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.399861.1, SRR1163655.424877.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158800 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTACC AGAGCTTGCG GCTGGAGTAC CTGCAGATCC CACCGGTCAG CCGCGCCTAC 
ACCACTGCCT GCGTCCTCAC CACCGCCGCC GTGCAGTTGG AATTGATCAC ACCTTTTCAG 
TTGTACTTCA ATCCTGAATT AATCTTTAAA CACTTTCAAG TATTGCAGTT GGACACATAT 
ATTTTTTCTT GGAAGATGTA TTTCCCAATC AACCTGGTGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291708.1
CDS	70..294
Translation	MAYQSLRLEYLQIPPVSRAYTTACVLTTAAVQLELITPFQLYFNPELIFKHFQVLQLDTYIFSWKMYFPINLVE

Target ORF information:

RefSeq Version	NM_001304779.1
Organism	Homo sapiens(human)
Definition	Homo sapiens derlin 2 (DERL2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304779.1

ORF Insert Sequence:

ATGGCGTACC AGAGCTTGCG GCTGGAGTAC CTGCAGATCC CACCGGTCAG CCGCGCCTAC 
ACCACTGCCT GCGTCCTCAC CACCGCCGCC GTGCAGTTGG AATTGATCAC ACCTTTTCAG 
TTGTACTTCA ATCCTGAATT AATCTTTAAA CACTTTCAAG TATTGCAGTT GGACACATAT 
ATTTTTTCTT GGAAGATGTA TTTCCCAATC AACCTGGTGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DERL2 ( NM_001304779.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001291708.1 Homo sapiens derlin 2 (DERL2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu67576
Clone ID Related Accession (Same CDS sequence)	NM_001304779.1 , NM_001304779.2
Accession Version	NM_001304779.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	derlin-2 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY051246.1, AF242523.1, BU199049.1, AC004148.1 and BM675359.1. On May 31, 2019 this sequence version replaced NM_001304779.1. Transcript Variant: This variant (3) lacks three alternate exons in the central coding region, resulting in a frameshift and a shorter 3' coding region, compared to variant 1. The encoded isoform (c) has a distinct C-terminus and is significantly shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.399861.1, SRR1163655.424877.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158800 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTACC AGAGCTTGCG GCTGGAGTAC CTGCAGATCC CACCGGTCAG CCGCGCCTAC 
ACCACTGCCT GCGTCCTCAC CACCGCCGCC GTGCAGTTGG AATTGATCAC ACCTTTTCAG 
TTGTACTTCA ATCCTGAATT AATCTTTAAA CACTTTCAAG TATTGCAGTT GGACACATAT 
ATTTTTTCTT GGAAGATGTA TTTCCCAATC AACCTGGTGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291708.1
CDS	18..242
Translation	MAYQSLRLEYLQIPPVSRAYTTACVLTTAAVQLELITPFQLYFNPELIFKHFQVLQLDTYIFSWKMYFPINLVE

Target ORF information:

RefSeq Version	NM_001304779.2
Organism	Homo sapiens(human)
Definition	Homo sapiens derlin 2 (DERL2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304779.2

ORF Insert Sequence:

ATGGCGTACC AGAGCTTGCG GCTGGAGTAC CTGCAGATCC CACCGGTCAG CCGCGCCTAC 
ACCACTGCCT GCGTCCTCAC CACCGCCGCC GTGCAGTTGG AATTGATCAC ACCTTTTCAG 
TTGTACTTCA ATCCTGAATT AATCTTTAAA CACTTTCAAG TATTGCAGTT GGACACATAT 
ATTTTTTCTT GGAAGATGTA TTTCCCAATC AACCTGGTGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Podocytes Exhibit a Specialized Protein Quality Control Employing Derlin-2 in Kidney Disease.
American journal of physiology. Renal physiologyajprenal006912016(2017 Nov)
Ren G,Tardi NJ,Matsuda F,Koh KH,Ruiz P,Wei C,Altintas MM,Ploegh H,Reiser J

Pre-emptive Quality Control Protects the ER from Protein Overload via the Proximity of ERAD Components and SRP.
Cell reports13(5)944-56(2015 Nov)
Kadowaki H,Nagai A,Maruyama T,Takami Y,Satrimafitrah P,Kato H,Honda A,Hatta T,Natsume T,Sato T,Kai H,Ichijo H,Nishitoh H

Identification of ERAD components essential for dislocation of the null Hong Kong variant of ?-1-antitrypsin (NHK).
Biochemical and biophysical research communications458(2)424-8(2015 Mar)
Zhong Y,Shen H,Wang Y,Yang Y,Yang P,Fang S

Proteasomal Degradation of Proinsulin Requires Derlin-2, HRD1 and p97.
PloS one10(6)e0128206(2015)
Hoelen H,Zaldumbide A,van Leeuwen WF,Torfs EC,Engelse MA,Hassan C,Lebbink RJ,de Koning EJ,Resssing ME,de Ru AH,van Veelen PA,Hoeben RC,Roep BO,Wiertz EJ

Derlin2 protein facilitates HRD1-mediated retro-translocation of sonic hedgehog at the endoplasmic reticulum.
The Journal of biological chemistry288(35)25330-9(2013 Aug)
Huang CH,Hsiao HT,Chu YR,Ye Y,Chen X

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DERL2 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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