PCCA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCCA
Entrez Gene ID	5095
Full Name	propionyl-CoA carboxylase alpha subunit
General protein information	Preferred Names propionyl-CoA carboxylase alpha subunit Names propionyl-CoA carboxylase alpha chain, mitochondrial PCCase alpha subunit pccA complementation group propanoyl-CoA:carbon dioxide ligase alpha subunit propionyl CoA carboxylase, alpha polypeptide propionyl Coenzyme A carboxylase, alpha polypeptide
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q32.3
Summary	The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010].
Disorder MIM:	232000
Disorder Html:	Propionicacidemia, 606054 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001352605.1	NP_001339534.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform d precursor
XM_017020609.1	XP_016876098.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X5
NM_000282.4	NP_000273.2	propionyl-CoA carboxylase alpha chain, mitochondrial isoform a precursor
NM_001352605.2	NP_001339534.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform d precursor
NM_001352612.1	NP_001339541.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform k
XM_017020618.1	XP_016876107.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X16
NM_000282.3	NP_000273.2	propionyl-CoA carboxylase alpha chain, mitochondrial isoform a precursor
NM_001352606.2	NP_001339535.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform e precursor
NM_001352612.2	NP_001339541.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform k
XM_017020605.1	XP_016876094.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X1
XM_017020613.1	XP_016876102.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X8
NM_001352611.1	NP_001339540.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform j
NM_001352607.1	NP_001339536.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform f
XM_017020610.1	XP_016876099.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X7
XM_017020617.1	XP_016876106.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X15
NM_001127692.2	NP_001121164.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform b
NM_001178004.1	NP_001171475.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform c precursor
NM_001352608.2	NP_001339537.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform g
NM_001352610.2	NP_001339539.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform i
XM_017020606.1	XP_016876095.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X2
XM_017020611.1	XP_016876100.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X6
XM_017020615.1	XP_016876104.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X9
XM_017020616.1	XP_016876105.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X10
XM_005254059.2	XP_005254116.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X12
NM_001352609.1	NP_001339538.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform h precursor
NM_001352606.1	NP_001339535.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform e precursor
NM_001352611.2	NP_001339540.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform j
NM_001352607.2	NP_001339536.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform f
XM_017020607.1	XP_016876096.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X3
XM_011521093.1	XP_011519395.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X4
NM_001352608.1	NP_001339537.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform g
NM_001352610.1	NP_001339539.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform i
XM_017020608.1	XP_016876097.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X4
XM_017020614.1	XP_016876103.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X11
XM_017020612.1	XP_016876101.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X7
NM_001352609.2	NP_001339538.1	propionyl-CoA carboxylase alpha chain, mitochondrial isoform h precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PCCA	XP_416970.2
Rattus norvegicus (Norway rat)	Pcca	NP_062203.1
Caenorhabditis elegans (roundworm)	pcca-1	NP_509293.1
Xenopus tropicalis (tropical clawed frog)	pcca	XP_002937132.1
Pan troglodytes (chimpanzee)	PCCA	XP_001149000.1
Canis lupus familiaris (dog)	PCCA	XP_534175.2
Homo sapiens (human)	PCCA	NP_000273.2
	PCCA	XP_001092670.1
Mus musculus (house mouse)	Pcca	NP_659093.2
Danio rerio (zebrafish)	pcca	NP_001002746.1
Bos taurus (cattle)	PCCA	NP_001076978.1

Gene Ontology
Bibliography

Related articles in PubMed

Seventeen Novel Mutations in PCCA and PCCB Genes in Indian Propionic Acidemia Patients, and Their Outcomes.
Gupta D, Bijarnia-Mahay S, Kohli S, Saxena R, Puri RD, Shigematsu Y, Yamaguchi S, Sakamoto O, Gupta N, Kabra M, Thakur S, Deb R, Verma IC
Genetic testing and molecular biomarkers20(7)373-82(2016 Jul)

Frameshift mutations of OGDH, PPAT and PCCA genes in gastric and colorectal cancers.
Jo YS, Oh HR, Kim MS, Yoo NJ, Lee SH
Neoplasma63(5)681-6(2016)

[Analysis of PCCA and PCCB gene mutations in patients with propionic acidemia].
Chen Z, Wen P, Wang G, Hu Y, Liu X, Chen L, Chen S, Wan L, Cui D, Shang Y, Li C
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics32(1)26-30(2015 Feb)

Two frequent mutations associated with the classic form of propionic acidemia in Taiwan.
Chiu YH, Liu YN, Liao WL, Chang YC, Lin SP, Hsu CC, Chiu PC, Niu DM, Wang CH, Ke YY, Chien YH, Hsiao KJ, Liu TT
Biochemical genetics52(9-10)415-29(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The majority of patients had mutations in the PCCA gene (18/25). A total of 26 mutations were noted: 20 in the PCCA gene and 6 in PCCB gene. Seventeen mutations were novel (14 in PCCA and 3 in PCCB). The SNP c.937C>T (p.Arg313Ter), was noted in 9/36 (25%) alleles in the PCCA gene
Title: Seventeen Novel Mutations in PCCA and PCCB Genes in Indian Propionic Acidemia Patients, and Their Outcomes.

Ten propionic acidemia mutations were confirmed, including 8 affecting the PCCA gene and 2 affecting the PCCB gene
Title: [Analysis of PCCA and PCCB gene mutations in patients with propionic acidemia].

Two PCCA mutations, c.229C-->T (p.R77W) and c.1262A-->C (p.Q421P), were identified in a PCCA-deficient patient.
Title: Two frequent mutations associated with the classic form of propionic acidemia in Taiwan.

Mutation analysis of the PCCA gene identified homozygous c.1284+1G>A in patient 1, c.230G>A (p.R77Q) and c.1855C>T (p.R619X) in patient 2, homozygous c.2125T>C (p.S709P) in patient 3, and only one mutant allele, c.231+1G>T in patient 4.
Title: Clinical characteristics and mutation analysis of propionic acidemia in Thailand.

Thsi study presented that Propionic acidemia(PCCA mutation) associated with visual hallucinations
Title: Propionic acidemia associated with visual hallucinations.

The following PCCA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCCA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22494	NM_000282.4 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu47381	NM_001352609.2 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu84566	NM_001352610.2 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu47381	XM_005254059.2 Latest version!	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu84566	XM_017020617.1 Latest version!	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu102858	NM_001352612.2 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu84567	NM_001352611.2 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu84567	XM_017020618.1 Latest version!	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu23088	NM_001127692.2 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu25085	NM_001178004.1 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu59515	XM_011521093.1 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu102895	NM_001352606.2 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu102909	NM_001352608.2 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu84557	NM_001352605.2 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84563	NM_001352607.2 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu84554	XM_017020605.1 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84555	XM_017020606.1 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84556	XM_017020607.1 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84557	XM_017020608.1 Latest version!	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84558	XM_017020609.1 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84559	XM_017020610.1 Latest version!	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84560	XM_017020611.1 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84561	XM_017020612.1 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84562	XM_017020613.1 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84563	XM_017020614.1 Latest version!	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu84564	XM_017020615.1 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu84565	XM_017020616.1 Latest version!	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu84557	NM_001352605.1	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu22494	NM_000282.3	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu102858	NM_001352612.1	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu102895	NM_001352606.1	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84567	NM_001352611.1	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu84563	NM_001352607.1	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu102909	NM_001352608.1	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu84566	NM_001352610.1	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu47381	NM_001352609.1	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCCA ( NM_000282.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000273.2 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22494
Clone ID Related Accession (Same CDS sequence)	NM_000282.4 , NM_000282.3
Accession Version	NM_000282.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF385926.1 and BC000140.2. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000282.3. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.106251.1, AF385926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000376285.6/ ENSP00000365462.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGT ACAAGGTGAA TATCTTAACC 
AGACTTGCCG CAGAATTGAA CAAATTTATG CTGGAAAAAG TGACTGAGGA CACAAGCAGT 
GTTCTGCGTT CCCCGATGCC CGGAGTGGTG GTGGCCGTCT CTGTCAAGCC TGGAGACGCG 
GTAGCAGAAG GTCAAGAAAT TTGTGTGATT GAAGCCATGA AAATGCAGAA TAGTATGACA 
GCTGGGAAAA CTGGCACGGT GAAATCTGTG CACTGTCAAG CTGGAGACAC AGTTGGAGAA 
GGGGATCTGC TCGTGGAGCT GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000273.2
CDS	29..2215
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_000282.4
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000282.4

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGT ACAAGGTGAA TATCTTAACC 
AGACTTGCCG CAGAATTGAA CAAATTTATG CTGGAAAAAG TGACTGAGGA CACAAGCAGT 
GTTCTGCGTT CCCCGATGCC CGGAGTGGTG GTGGCCGTCT CTGTCAAGCC TGGAGACGCG 
GTAGCAGAAG GTCAAGAAAT TTGTGTGATT GAAGCCATGA AAATGCAGAA TAGTATGACA 
GCTGGGAAAA CTGGCACGGT GAAATCTGTG CACTGTCAAG CTGGAGACAC AGTTGGAGAA 
GGGGATCTGC TCGTGGAGCT GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_000282.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000273.2 Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant 1, mRNA.

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CloneID	OHu22494
Clone ID Related Accession (Same CDS sequence)	NM_000282.4 , NM_000282.3
Accession Version	NM_000282.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AF385926.1, BC000140.2 and CB851764.1. This sequence is a reference standard in the RefSeqGene project. On May 6, 2010 this sequence version replaced NM_000282.2. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.20816.1, SRR1660803.168289.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGT ACAAGGTGAA TATCTTAACC 
AGACTTGCCG CAGAATTGAA CAAATTTATG CTGGAAAAAG TGACTGAGGA CACAAGCAGT 
GTTCTGCGTT CCCCGATGCC CGGAGTGGTG GTGGCCGTCT CTGTCAAGCC TGGAGACGCG 
GTAGCAGAAG GTCAAGAAAT TTGTGTGATT GAAGCCATGA AAATGCAGAA TAGTATGACA 
GCTGGGAAAA CTGGCACGGT GAAATCTGTG CACTGTCAAG CTGGAGACAC AGTTGGAGAA 
GGGGATCTGC TCGTGGAGCT GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000273.2
CDS	107..2293
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_000282.3
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000282.3

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGT ACAAGGTGAA TATCTTAACC 
AGACTTGCCG CAGAATTGAA CAAATTTATG CTGGAAAAAG TGACTGAGGA CACAAGCAGT 
GTTCTGCGTT CCCCGATGCC CGGAGTGGTG GTGGCCGTCT CTGTCAAGCC TGGAGACGCG 
GTAGCAGAAG GTCAAGAAAT TTGTGTGATT GAAGCCATGA AAATGCAGAA TAGTATGACA 
GCTGGGAAAA CTGGCACGGT GAAATCTGTG CACTGTCAAG CTGGAGACAC AGTTGGAGAA 
GGGGATCTGC TCGTGGAGCT GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352609.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339538.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu47381
Clone ID Related Accession (Same CDS sequence)	XM_005254059.2 , NM_001352609.1 , NM_001352609.2
Accession Version	NM_001352609.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform h precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. On Jun 18, 2017 this sequence version replaced XM_005254059.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.169854.1, SRR1803616.66329.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGC CCTGGAGAAA GCTTCTGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339538.1
CDS	107..2029
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVPWRKLLD

Target ORF information:

RefSeq Version	NM_001352609.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352609.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGC CCTGGAGAAA GCTTCTGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352609.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339538.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu47381
Clone ID Related Accession (Same CDS sequence)	XM_005254059.2 , NM_001352609.1 , NM_001352609.2
Accession Version	NM_001352609.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform h precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. On May 31, 2019 this sequence version replaced NM_001352609.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.66329.1, SRR1803616.227189.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGC CCTGGAGAAA GCTTCTGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339538.1
CDS	29..1951
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVPWRKLLD

Target ORF information:

RefSeq Version	NM_001352609.2
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352609.2

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGC CCTGGAGAAA GCTTCTGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352610.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339539.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 9, mRNA.

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CloneID	OHu84566
Clone ID Related Accession (Same CDS sequence)	XM_017020617.1 , NM_001352610.2 , NM_001352610.1
Accession Version	NM_001352610.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. On May 31, 2019 this sequence version replaced NM_001352610.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.24632.1, SRR1803611.215420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
TGTCTTTCTC GAGAAGCAGG TGGAAACATG AGCATTCAGT TTCTTGGTAC AGTGTACAAG 
GTGAATATCT TAACCAGACT TGCCGCAGAA TTGAACAAAT TTATGCTGGA AAAAGTGACT 
GAGGACACAA GCAGTGTTCT GCGTTCCCCG ATGCCCGGAG TGGTGGTGGC CGTCTCTGTC 
AAGCCTGGAG ACGCGGTAGC AGAAGGTCAA GAAATTTGTG TGATTGAAGC CATGAAAATG 
CAGAATAGTA TGACAGCTGG GAAAACTGGC ACGGTGAAAT CTGTGCACTG TCAAGCTGGA 
GACACAGTTG GAGAAGGGGA TCTGCTCGTG GAGCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339539.1
CDS	895..2136
Translation	MGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352610.2
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352610.2

ORF Insert Sequence:

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
TGTCTTTCTC GAGAAGCAGG TGGAAACATG AGCATTCAGT TTCTTGGTAC AGTGTACAAG 
GTGAATATCT TAACCAGACT TGCCGCAGAA TTGAACAAAT TTATGCTGGA AAAAGTGACT 
GAGGACACAA GCAGTGTTCT GCGTTCCCCG ATGCCCGGAG TGGTGGTGGC CGTCTCTGTC 
AAGCCTGGAG ACGCGGTAGC AGAAGGTCAA GAAATTTGTG TGATTGAAGC CATGAAAATG 
CAGAATAGTA TGACAGCTGG GAAAACTGGC ACGGTGAAAT CTGTGCACTG TCAAGCTGGA 
GACACAGTTG GAGAAGGGGA TCTGCTCGTG GAGCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352610.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339539.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 9, mRNA.

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CloneID	OHu84566
Clone ID Related Accession (Same CDS sequence)	XM_017020617.1 , NM_001352610.2 , NM_001352610.1
Accession Version	NM_001352610.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. On Jun 18, 2017 this sequence version replaced XM_017020617.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.215420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
TGTCTTTCTC GAGAAGCAGG TGGAAACATG AGCATTCAGT TTCTTGGTAC AGTGTACAAG 
GTGAATATCT TAACCAGACT TGCCGCAGAA TTGAACAAAT TTATGCTGGA AAAAGTGACT 
GAGGACACAA GCAGTGTTCT GCGTTCCCCG ATGCCCGGAG TGGTGGTGGC CGTCTCTGTC 
AAGCCTGGAG ACGCGGTAGC AGAAGGTCAA GAAATTTGTG TGATTGAAGC CATGAAAATG 
CAGAATAGTA TGACAGCTGG GAAAACTGGC ACGGTGAAAT CTGTGCACTG TCAAGCTGGA 
GACACAGTTG GAGAAGGGGA TCTGCTCGTG GAGCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339539.1
CDS	973..2214
Translation	MGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352610.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352610.1

ORF Insert Sequence:

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
TGTCTTTCTC GAGAAGCAGG TGGAAACATG AGCATTCAGT TTCTTGGTAC AGTGTACAAG 
GTGAATATCT TAACCAGACT TGCCGCAGAA TTGAACAAAT TTATGCTGGA AAAAGTGACT 
GAGGACACAA GCAGTGTTCT GCGTTCCCCG ATGCCCGGAG TGGTGGTGGC CGTCTCTGTC 
AAGCCTGGAG ACGCGGTAGC AGAAGGTCAA GAAATTTGTG TGATTGAAGC CATGAAAATG 
CAGAATAGTA TGACAGCTGG GAAAACTGGC ACGGTGAAAT CTGTGCACTG TCAAGCTGGA 
GACACAGTTG GAGAAGGGGA TCTGCTCGTG GAGCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_005254059.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254116.1 Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X15, mRNA.

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CloneID	OHu47381
Clone ID Related Accession (Same CDS sequence)	XM_005254059.2 , NM_001352609.1 , NM_001352609.2
Accession Version	XM_005254059.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005254059.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGC CCTGGAGAAA GCTTCTGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254116.1
CDS	102..2024
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVPWRKLLD

Target ORF information:

RefSeq Version	XM_005254059.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254059.2

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGC CCTGGAGAAA GCTTCTGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020617.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876106.1 Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X23, mRNA.

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CloneID	OHu84566
Clone ID Related Accession (Same CDS sequence)	XM_017020617.1 , NM_001352610.2 , NM_001352610.1
Accession Version	XM_017020617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
TGTCTTTCTC GAGAAGCAGG TGGAAACATG AGCATTCAGT TTCTTGGTAC AGTGTACAAG 
GTGAATATCT TAACCAGACT TGCCGCAGAA TTGAACAAAT TTATGCTGGA AAAAGTGACT 
GAGGACACAA GCAGTGTTCT GCGTTCCCCG ATGCCCGGAG TGGTGGTGGC CGTCTCTGTC 
AAGCCTGGAG ACGCGGTAGC AGAAGGTCAA GAAATTTGTG TGATTGAAGC CATGAAAATG 
CAGAATAGTA TGACAGCTGG GAAAACTGGC ACGGTGAAAT CTGTGCACTG TCAAGCTGGA 
GACACAGTTG GAGAAGGGGA TCTGCTCGTG GAGCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876106.1
CDS	631..1872
Translation	MGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	XM_017020617.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020617.1

ORF Insert Sequence:

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
TGTCTTTCTC GAGAAGCAGG TGGAAACATG AGCATTCAGT TTCTTGGTAC AGTGTACAAG 
GTGAATATCT TAACCAGACT TGCCGCAGAA TTGAACAAAT TTATGCTGGA AAAAGTGACT 
GAGGACACAA GCAGTGTTCT GCGTTCCCCG ATGCCCGGAG TGGTGGTGGC CGTCTCTGTC 
AAGCCTGGAG ACGCGGTAGC AGAAGGTCAA GAAATTTGTG TGATTGAAGC CATGAAAATG 
CAGAATAGTA TGACAGCTGG GAAAACTGGC ACGGTGAAAT CTGTGCACTG TCAAGCTGGA 
GACACAGTTG GAGAAGGGGA TCTGCTCGTG GAGCTGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352612.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339541.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 11, mRNA.

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CloneID	OHu102858
Clone ID Related Accession (Same CDS sequence)	NM_001352612.1 , NM_001352612.2
Accession Version	NM_001352612.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform k
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.233926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GACCCCTACA AGTCTTTTGG TTTACCATCT ATTGGGAGAT TGTCTCAGTA CCAAGAACCG 
TTACATCTAC CTGGTGTCCG AGTGGACAGT GGCATCCAAC CAGGAAGTGA TATTAGCATT 
TATTATGATC CTATGATTTC AAAACTAATC ACATATGGCT CTGATAGAAC TGAGGCACTG 
AAGAGAATGG CAGATGCACT GGATAACTAT GTTATTCGAG GTGTTACACA TAATATTGCA 
TTACTTCGAG AGGTGATAAT CAACTCACGC TTTGTAAAAG GAGACATCAG CACTAAATTT 
CTCTCCGATG TGTATCCTGA TGGCTTCAAA GGACACATGC TAACCAAGAG TGAGAAGAAC 
CAGTTATTGG CAATAGCATC ATCATTGTTT GTGGCATTCC AGTTAAGAGC ACAACATTTT 
CAAGAAAATT CAAGAATGCC TGTTATTAAA CCAGACATAG CCAACTGGGA GCTCTCAGTA 
AAATTGCATG ATAAAGTTCA TACCGTAGTA GCATCAAACA ATGGGTCAGT GTTCTCGGTG 
GAAGTTGATG GGTCGAAACT AAATGTGACC AGCACGTGGA ACCTGGCTTC GCCCTTATTG 
TCTGTCAGCG TTGATGGCAC TCAGAGGACT GTCCAGTGTC TTTCTCGAGA AGCAGGTGGA 
AACATGAGCA TTCAGTTTCT TGGTACAGTG TACAAGGTGA ATATCTTAAC CAGACTTGCC 
GCAGAATTGA ACAAATTTAT GCTGGAAAAA GTGACTGAGG ACACAAGCAG TGTTCTGCGT 
TCCCCGATGC CCGGAGTGGT GGTGGCCGTC TCTGTCAAGC CTGGAGACGC GGTAGCAGAA 
GGTCAAGAAA TTTGTGTGAT TGAAGCCATG AAAATGCAGA ATAGTATGAC AGCTGGGAAA 
ACTGGCACGG TGAAATCTGT GCACTGTCAA GCTGGAGACA CAGTTGGAGA AGGGGATCTG 
CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339541.1
CDS	973..2070
Translation	MGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352612.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352612.1

ORF Insert Sequence:

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GACCCCTACA AGTCTTTTGG TTTACCATCT ATTGGGAGAT TGTCTCAGTA CCAAGAACCG 
TTACATCTAC CTGGTGTCCG AGTGGACAGT GGCATCCAAC CAGGAAGTGA TATTAGCATT 
TATTATGATC CTATGATTTC AAAACTAATC ACATATGGCT CTGATAGAAC TGAGGCACTG 
AAGAGAATGG CAGATGCACT GGATAACTAT GTTATTCGAG GTGTTACACA TAATATTGCA 
TTACTTCGAG AGGTGATAAT CAACTCACGC TTTGTAAAAG GAGACATCAG CACTAAATTT 
CTCTCCGATG TGTATCCTGA TGGCTTCAAA GGACACATGC TAACCAAGAG TGAGAAGAAC 
CAGTTATTGG CAATAGCATC ATCATTGTTT GTGGCATTCC AGTTAAGAGC ACAACATTTT 
CAAGAAAATT CAAGAATGCC TGTTATTAAA CCAGACATAG CCAACTGGGA GCTCTCAGTA 
AAATTGCATG ATAAAGTTCA TACCGTAGTA GCATCAAACA ATGGGTCAGT GTTCTCGGTG 
GAAGTTGATG GGTCGAAACT AAATGTGACC AGCACGTGGA ACCTGGCTTC GCCCTTATTG 
TCTGTCAGCG TTGATGGCAC TCAGAGGACT GTCCAGTGTC TTTCTCGAGA AGCAGGTGGA 
AACATGAGCA TTCAGTTTCT TGGTACAGTG TACAAGGTGA ATATCTTAAC CAGACTTGCC 
GCAGAATTGA ACAAATTTAT GCTGGAAAAA GTGACTGAGG ACACAAGCAG TGTTCTGCGT 
TCCCCGATGC CCGGAGTGGT GGTGGCCGTC TCTGTCAAGC CTGGAGACGC GGTAGCAGAA 
GGTCAAGAAA TTTGTGTGAT TGAAGCCATG AAAATGCAGA ATAGTATGAC AGCTGGGAAA 
ACTGGCACGG TGAAATCTGT GCACTGTCAA GCTGGAGACA CAGTTGGAGA AGGGGATCTG 
CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352612.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339541.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 11, mRNA.

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CloneID	OHu102858
Clone ID Related Accession (Same CDS sequence)	NM_001352612.1 , NM_001352612.2
Accession Version	NM_001352612.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform k
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. On May 31, 2019 this sequence version replaced NM_001352612.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.233926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GACCCCTACA AGTCTTTTGG TTTACCATCT ATTGGGAGAT TGTCTCAGTA CCAAGAACCG 
TTACATCTAC CTGGTGTCCG AGTGGACAGT GGCATCCAAC CAGGAAGTGA TATTAGCATT 
TATTATGATC CTATGATTTC AAAACTAATC ACATATGGCT CTGATAGAAC TGAGGCACTG 
AAGAGAATGG CAGATGCACT GGATAACTAT GTTATTCGAG GTGTTACACA TAATATTGCA 
TTACTTCGAG AGGTGATAAT CAACTCACGC TTTGTAAAAG GAGACATCAG CACTAAATTT 
CTCTCCGATG TGTATCCTGA TGGCTTCAAA GGACACATGC TAACCAAGAG TGAGAAGAAC 
CAGTTATTGG CAATAGCATC ATCATTGTTT GTGGCATTCC AGTTAAGAGC ACAACATTTT 
CAAGAAAATT CAAGAATGCC TGTTATTAAA CCAGACATAG CCAACTGGGA GCTCTCAGTA 
AAATTGCATG ATAAAGTTCA TACCGTAGTA GCATCAAACA ATGGGTCAGT GTTCTCGGTG 
GAAGTTGATG GGTCGAAACT AAATGTGACC AGCACGTGGA ACCTGGCTTC GCCCTTATTG 
TCTGTCAGCG TTGATGGCAC TCAGAGGACT GTCCAGTGTC TTTCTCGAGA AGCAGGTGGA 
AACATGAGCA TTCAGTTTCT TGGTACAGTG TACAAGGTGA ATATCTTAAC CAGACTTGCC 
GCAGAATTGA ACAAATTTAT GCTGGAAAAA GTGACTGAGG ACACAAGCAG TGTTCTGCGT 
TCCCCGATGC CCGGAGTGGT GGTGGCCGTC TCTGTCAAGC CTGGAGACGC GGTAGCAGAA 
GGTCAAGAAA TTTGTGTGAT TGAAGCCATG AAAATGCAGA ATAGTATGAC AGCTGGGAAA 
ACTGGCACGG TGAAATCTGT GCACTGTCAA GCTGGAGACA CAGTTGGAGA AGGGGATCTG 
CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339541.1
CDS	895..1992
Translation	MGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352612.2
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352612.2

ORF Insert Sequence:

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GACCCCTACA AGTCTTTTGG TTTACCATCT ATTGGGAGAT TGTCTCAGTA CCAAGAACCG 
TTACATCTAC CTGGTGTCCG AGTGGACAGT GGCATCCAAC CAGGAAGTGA TATTAGCATT 
TATTATGATC CTATGATTTC AAAACTAATC ACATATGGCT CTGATAGAAC TGAGGCACTG 
AAGAGAATGG CAGATGCACT GGATAACTAT GTTATTCGAG GTGTTACACA TAATATTGCA 
TTACTTCGAG AGGTGATAAT CAACTCACGC TTTGTAAAAG GAGACATCAG CACTAAATTT 
CTCTCCGATG TGTATCCTGA TGGCTTCAAA GGACACATGC TAACCAAGAG TGAGAAGAAC 
CAGTTATTGG CAATAGCATC ATCATTGTTT GTGGCATTCC AGTTAAGAGC ACAACATTTT 
CAAGAAAATT CAAGAATGCC TGTTATTAAA CCAGACATAG CCAACTGGGA GCTCTCAGTA 
AAATTGCATG ATAAAGTTCA TACCGTAGTA GCATCAAACA ATGGGTCAGT GTTCTCGGTG 
GAAGTTGATG GGTCGAAACT AAATGTGACC AGCACGTGGA ACCTGGCTTC GCCCTTATTG 
TCTGTCAGCG TTGATGGCAC TCAGAGGACT GTCCAGTGTC TTTCTCGAGA AGCAGGTGGA 
AACATGAGCA TTCAGTTTCT TGGTACAGTG TACAAGGTGA ATATCTTAAC CAGACTTGCC 
GCAGAATTGA ACAAATTTAT GCTGGAAAAA GTGACTGAGG ACACAAGCAG TGTTCTGCGT 
TCCCCGATGC CCGGAGTGGT GGTGGCCGTC TCTGTCAAGC CTGGAGACGC GGTAGCAGAA 
GGTCAAGAAA TTTGTGTGAT TGAAGCCATG AAAATGCAGA ATAGTATGAC AGCTGGGAAA 
ACTGGCACGG TGAAATCTGT GCACTGTCAA GCTGGAGACA CAGTTGGAGA AGGGGATCTG 
CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352611.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339540.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 10, mRNA.

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CloneID	OHu84567
Clone ID Related Accession (Same CDS sequence)	XM_017020618.1 , NM_001352611.1 , NM_001352611.2
Accession Version	NM_001352611.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform j
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. On Jun 18, 2017 this sequence version replaced XM_017020618.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.67161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
TACAAGGTGA ATATCTTAAC CAGACTTGCC GCAGAATTGA ACAAATTTAT GCTGGAAAAA 
GTGACTGAGG ACACAAGCAG TGTTCTGCGT TCCCCGATGC CCGGAGTGGT GGTGGCCGTC 
TCTGTCAAGC CTGGAGACGC GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339540.1
CDS	973..2160
Translation	MGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352611.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352611.1

ORF Insert Sequence:

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
TACAAGGTGA ATATCTTAAC CAGACTTGCC GCAGAATTGA ACAAATTTAT GCTGGAAAAA 
GTGACTGAGG ACACAAGCAG TGTTCTGCGT TCCCCGATGC CCGGAGTGGT GGTGGCCGTC 
TCTGTCAAGC CTGGAGACGC GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352611.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339540.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 10, mRNA.

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CloneID	OHu84567
Clone ID Related Accession (Same CDS sequence)	XM_017020618.1 , NM_001352611.1 , NM_001352611.2
Accession Version	NM_001352611.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform j
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. On May 31, 2019 this sequence version replaced NM_001352611.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.67161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
TACAAGGTGA ATATCTTAAC CAGACTTGCC GCAGAATTGA ACAAATTTAT GCTGGAAAAA 
GTGACTGAGG ACACAAGCAG TGTTCTGCGT TCCCCGATGC CCGGAGTGGT GGTGGCCGTC 
TCTGTCAAGC CTGGAGACGC GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339540.1
CDS	895..2082
Translation	MGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352611.2
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352611.2

ORF Insert Sequence:

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
TACAAGGTGA ATATCTTAAC CAGACTTGCC GCAGAATTGA ACAAATTTAT GCTGGAAAAA 
GTGACTGAGG ACACAAGCAG TGTTCTGCGT TCCCCGATGC CCGGAGTGGT GGTGGCCGTC 
TCTGTCAAGC CTGGAGACGC GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020618.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876107.1 Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OHu84567
Clone ID Related Accession (Same CDS sequence)	XM_017020618.1 , NM_001352611.1 , NM_001352611.2
Accession Version	XM_017020618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
TACAAGGTGA ATATCTTAAC CAGACTTGCC GCAGAATTGA ACAAATTTAT GCTGGAAAAA 
GTGACTGAGG ACACAAGCAG TGTTCTGCGT TCCCCGATGC CCGGAGTGGT GGTGGCCGTC 
TCTGTCAAGC CTGGAGACGC GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876107.1
CDS	631..1818
Translation	MGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	XM_017020618.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020618.1

ORF Insert Sequence:

ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
TACAAGGTGA ATATCTTAAC CAGACTTGCC GCAGAATTGA ACAAATTTAT GCTGGAAAAA 
GTGACTGAGG ACACAAGCAG TGTTCTGCGT TCCCCGATGC CCGGAGTGGT GGTGGCCGTC 
TCTGTCAAGC CTGGAGACGC GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001127692.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121164.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu23088
Clone ID Related Accession (Same CDS sequence)	NM_001127692.2
Accession Version	NM_001127692.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AF385926.1, DA781564.1, BC000140.2 and CB851764.1. On May 6, 2010 this sequence version replaced NM_001127692.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to NM_000282.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.23215.1, SRR1660803.25828.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGTT GAGCATCCTG TCACAGAATG CATTACTGGC 
CTGGACCTAG TCCAGGAAAT GATCCGTGTT GCTAAGGGCT ACCCTCTCAG GCACAAACAA 
GCTGATATTC GCATCAACGG CTGGGCAGTT GAATGTCGGG TTTATGCTGA GGACCCCTAC 
AAGTCTTTTG GTTTACCATC TATTGGGAGA TTGTCTCAGT ACCAAGAACC GTTACATCTA 
CCTGGTGTCC GAGTGGACAG TGGCATCCAA CCAGGAAGTG ATATTAGCAT TTATTATGAT 
CCTATGATTT CAAAACTAAT CACATATGGC TCTGATAGAA CTGAGGCACT GAAGAGAATG 
GCAGATGCAC TGGATAACTA TGTTATTCGA GGTGTTACAC ATAATATTGC ATTACTTCGA 
GAGGTGATAA TCAACTCACG CTTTGTAAAA GGAGACATCA GCACTAAATT TCTCTCCGAT 
GTGTATCCTG ATGGCTTCAA AGGACACATG CTAACCAAGA GTGAGAAGAA CCAGTTATTG 
GCAATAGCAT CATCATTGTT TGTGGCATTC CAGTTAAGAG CACAACATTT TCAAGAAAAT 
TCAAGAATGC CTGTTATTAA ACCAGACATA GCCAACTGGG AGCTCTCAGT AAAATTGCAT 
GATAAAGTTC ATACCGTAGT AGCATCAAAC AATGGGTCAG TGTTCTCGGT GGAAGTTGAT 
GGGTCGAAAC TAAATGTGAC CAGCACGTGG AACCTGGCTT CGCCCTTATT GTCTGTCAGC 
GTTGATGGCA CTCAGAGGAC TGTCCAGTGT CTTTCTCGAG AAGCAGGTGG AAACATGAGC 
ATTCAGTTTC TTGGTACAGT GTACAAGGTG AATATCTTAA CCAGACTTGC CGCAGAATTG 
AACAAATTTA TGCTGGAAAA AGTGACTGAG GACACAAGCA GTGTTCTGCG TTCCCCGATG 
CCCGGAGTGG TGGTGGCCGT CTCTGTCAAG CCTGGAGACG CGGTAGCAGA AGGTCAAGAA 
ATTTGTGTGA TTGAAGCCAT GAAAATGCAG AATAGTATGA CAGCTGGGAA AACTGGCACG 
GTGAAATCTG TGCACTGTCA AGCTGGAGAC ACAGTTGGAG AAGGGGATCT GCTCGTGGAG 
CTGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121164.1
CDS	107..2215
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001127692.2
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127692.2

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGTT GAGCATCCTG TCACAGAATG CATTACTGGC 
CTGGACCTAG TCCAGGAAAT GATCCGTGTT GCTAAGGGCT ACCCTCTCAG GCACAAACAA 
GCTGATATTC GCATCAACGG CTGGGCAGTT GAATGTCGGG TTTATGCTGA GGACCCCTAC 
AAGTCTTTTG GTTTACCATC TATTGGGAGA TTGTCTCAGT ACCAAGAACC GTTACATCTA 
CCTGGTGTCC GAGTGGACAG TGGCATCCAA CCAGGAAGTG ATATTAGCAT TTATTATGAT 
CCTATGATTT CAAAACTAAT CACATATGGC TCTGATAGAA CTGAGGCACT GAAGAGAATG 
GCAGATGCAC TGGATAACTA TGTTATTCGA GGTGTTACAC ATAATATTGC ATTACTTCGA 
GAGGTGATAA TCAACTCACG CTTTGTAAAA GGAGACATCA GCACTAAATT TCTCTCCGAT 
GTGTATCCTG ATGGCTTCAA AGGACACATG CTAACCAAGA GTGAGAAGAA CCAGTTATTG 
GCAATAGCAT CATCATTGTT TGTGGCATTC CAGTTAAGAG CACAACATTT TCAAGAAAAT 
TCAAGAATGC CTGTTATTAA ACCAGACATA GCCAACTGGG AGCTCTCAGT AAAATTGCAT 
GATAAAGTTC ATACCGTAGT AGCATCAAAC AATGGGTCAG TGTTCTCGGT GGAAGTTGAT 
GGGTCGAAAC TAAATGTGAC CAGCACGTGG AACCTGGCTT CGCCCTTATT GTCTGTCAGC 
GTTGATGGCA CTCAGAGGAC TGTCCAGTGT CTTTCTCGAG AAGCAGGTGG AAACATGAGC 
ATTCAGTTTC TTGGTACAGT GTACAAGGTG AATATCTTAA CCAGACTTGC CGCAGAATTG 
AACAAATTTA TGCTGGAAAA AGTGACTGAG GACACAAGCA GTGTTCTGCG TTCCCCGATG 
CCCGGAGTGG TGGTGGCCGT CTCTGTCAAG CCTGGAGACG CGGTAGCAGA AGGTCAAGAA 
ATTTGTGTGA TTGAAGCCAT GAAAATGCAG AATAGTATGA CAGCTGGGAA AACTGGCACG 
GTGAAATCTG TGCACTGTCA AGCTGGAGAC ACAGTTGGAG AAGGGGATCT GCTCGTGGAG 
CTGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001178004.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171475.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 3, mRNA.

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CloneID	OHu25085
Clone ID Related Accession (Same CDS sequence)	NM_001178004.1
Accession Version	NM_001178004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AK298318.1, AL356575.8 and CB851764.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The resulting protein (isoform c) is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.11500.1, SRR1803615.47545.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGG TAGCAGAAGG TCAAGAAATT 
TGTGTGATTG AAGCCATGAA AATGCAGAAT AGTATGACAG CTGGGAAAAC TGGCACGGTG 
AAATCTGTGC ACTGTCAAGC TGGAGACACA GTTGGAGAAG GGGATCTGCT CGTGGAGCTG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171475.1
CDS	107..2152
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001178004.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178004.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGG TAGCAGAAGG TCAAGAAATT 
TGTGTGATTG AAGCCATGAA AATGCAGAAT AGTATGACAG CTGGGAAAAC TGGCACGGTG 
AAATCTGTGC ACTGTCAAGC TGGAGACACA GTTGGAGAAG GGGATCTGCT CGTGGAGCTG 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_011521093.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519395.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu59515
Clone ID Related Accession (Same CDS sequence)	XM_011521093.1
Accession Version	XM_011521093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGG AGCACGGGGC TCAGGGAAAG 
TTCAGCATTG TCAGTTGTCA ACAAAATCAC CCTGGAGAAA GCTTCTGGAT TGACTGTGAG 
AAGCTTGGCA GAAGGTGCTG CGGCCTGGTG GCTGGCTCAG CAGGAGGCCT GGGATTGAAA 
GCTCTCGGTT TGTACAAGCT CTTTTGGATT TGTAACACTT TTATTTTAAA TCTCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519395.1
CDS	102..2201
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVEHGAQGKFSIVSCQQNHPGESFWIDCEKLGRRCCGLVAGSAGGLGLKALGLYKLFWICNTFILNLQ

Target ORF information:

RefSeq Version	XM_011521093.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521093.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTGTCT TTCTCGAGAA 
GCAGGTGGAA ACATGAGCAT TCAGTTTCTT GGTACAGTGG AGCACGGGGC TCAGGGAAAG 
TTCAGCATTG TCAGTTGTCA ACAAAATCAC CCTGGAGAAA GCTTCTGGAT TGACTGTGAG 
AAGCTTGGCA GAAGGTGCTG CGGCCTGGTG GCTGGCTCAG CAGGAGGCCT GGGATTGAAA 
GCTCTCGGTT TGTACAAGCT CTTTTGGATT TGTAACACTT TTATTTTAAA TCTCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352606.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339535.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu102895
Clone ID Related Accession (Same CDS sequence)	NM_001352606.2 , NM_001352606.1
Accession Version	NM_001352606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform e precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. On May 31, 2019 this sequence version replaced NM_001352606.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.133469.1, SRR1803617.199738.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGACCC CTACAAGTCT 
TTTGGTTTAC CATCTATTGG GAGATTGTCT CAGTACCAAG AACCGTTACA TCTACCTGGT 
GTCCGAGTGG ACAGTGGCAT CCAACCAGGA AGTGATATTA GCATTTATTA TGATCCTATG 
ATTTCAAAAC TAATCACATA TGGCTCTGAT AGAACTGAGG CACTGAAGAG AATGGCAGAT 
GCACTGGATA ACTATGTTAT TCGAGGTGTT ACACATAATA TTGCATTACT TCGAGAGGTG 
ATAATCAACT CACGCTTTGT AAAAGGAGAC ATCAGCACTA AATTTCTCTC CGATGTGTAT 
CCTGATGGCT TCAAAGGACA CATGCTAACC AAGAGTGAGA AGAACCAGTT ATTGGCAATA 
GCATCATCAT TGTTTGTGGC ATTCCAGTTA AGAGCACAAC ATTTTCAAGA AAATTCAAGA 
ATGCCTGTTA TTAAACCAGA CATAGCCAAC TGGGAGCTCT CAGTAAAATT GCATGATAAA 
GTTCATACCG TAGTAGCATC AAACAATGGG TCAGTGTTCT CGGTGGAAGT TGATGGGTCG 
AAACTAAATG TGACCAGCAC GTGGAACCTG GCTTCGCCCT TATTGTCTGT CAGCGTTGAT 
GGCACTCAGA GGACTGTCCA GTGTCTTTCT CGAGAAGCAG GTGGAAACAT GAGCATTCAG 
TTTCTTGGTA CAGTGTACAA GGTGAATATC TTAACCAGAC TTGCCGCAGA ATTGAACAAA 
TTTATGCTGG AAAAAGTGAC TGAGGACACA AGCAGTGTTC TGCGTTCCCC GATGCCCGGA 
GTGGTGGTGG CCGTCTCTGT CAAGCCTGGA GACGCGGTAG CAGAAGGTCA AGAAATTTGT 
GTGATTGAAG CCATGAAAAT GCAGAATAGT ATGACAGCTG GGAAAACTGG CACGGTGAAA 
TCTGTGCACT GTCAAGCTGG AGACACAGTT GGAGAAGGGG ATCTGCTCGT GGAGCTGGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339535.1
CDS	29..2071
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352606.2
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352606.2

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGACCC CTACAAGTCT 
TTTGGTTTAC CATCTATTGG GAGATTGTCT CAGTACCAAG AACCGTTACA TCTACCTGGT 
GTCCGAGTGG ACAGTGGCAT CCAACCAGGA AGTGATATTA GCATTTATTA TGATCCTATG 
ATTTCAAAAC TAATCACATA TGGCTCTGAT AGAACTGAGG CACTGAAGAG AATGGCAGAT 
GCACTGGATA ACTATGTTAT TCGAGGTGTT ACACATAATA TTGCATTACT TCGAGAGGTG 
ATAATCAACT CACGCTTTGT AAAAGGAGAC ATCAGCACTA AATTTCTCTC CGATGTGTAT 
CCTGATGGCT TCAAAGGACA CATGCTAACC AAGAGTGAGA AGAACCAGTT ATTGGCAATA 
GCATCATCAT TGTTTGTGGC ATTCCAGTTA AGAGCACAAC ATTTTCAAGA AAATTCAAGA 
ATGCCTGTTA TTAAACCAGA CATAGCCAAC TGGGAGCTCT CAGTAAAATT GCATGATAAA 
GTTCATACCG TAGTAGCATC AAACAATGGG TCAGTGTTCT CGGTGGAAGT TGATGGGTCG 
AAACTAAATG TGACCAGCAC GTGGAACCTG GCTTCGCCCT TATTGTCTGT CAGCGTTGAT 
GGCACTCAGA GGACTGTCCA GTGTCTTTCT CGAGAAGCAG GTGGAAACAT GAGCATTCAG 
TTTCTTGGTA CAGTGTACAA GGTGAATATC TTAACCAGAC TTGCCGCAGA ATTGAACAAA 
TTTATGCTGG AAAAAGTGAC TGAGGACACA AGCAGTGTTC TGCGTTCCCC GATGCCCGGA 
GTGGTGGTGG CCGTCTCTGT CAAGCCTGGA GACGCGGTAG CAGAAGGTCA AGAAATTTGT 
GTGATTGAAG CCATGAAAAT GCAGAATAGT ATGACAGCTG GGAAAACTGG CACGGTGAAA 
TCTGTGCACT GTCAAGCTGG AGACACAGTT GGAGAAGGGG ATCTGCTCGT GGAGCTGGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352606.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339535.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu102895
Clone ID Related Accession (Same CDS sequence)	NM_001352606.2 , NM_001352606.1
Accession Version	NM_001352606.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform e precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.199738.1, SRR1803611.133469.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGACCC CTACAAGTCT 
TTTGGTTTAC CATCTATTGG GAGATTGTCT CAGTACCAAG AACCGTTACA TCTACCTGGT 
GTCCGAGTGG ACAGTGGCAT CCAACCAGGA AGTGATATTA GCATTTATTA TGATCCTATG 
ATTTCAAAAC TAATCACATA TGGCTCTGAT AGAACTGAGG CACTGAAGAG AATGGCAGAT 
GCACTGGATA ACTATGTTAT TCGAGGTGTT ACACATAATA TTGCATTACT TCGAGAGGTG 
ATAATCAACT CACGCTTTGT AAAAGGAGAC ATCAGCACTA AATTTCTCTC CGATGTGTAT 
CCTGATGGCT TCAAAGGACA CATGCTAACC AAGAGTGAGA AGAACCAGTT ATTGGCAATA 
GCATCATCAT TGTTTGTGGC ATTCCAGTTA AGAGCACAAC ATTTTCAAGA AAATTCAAGA 
ATGCCTGTTA TTAAACCAGA CATAGCCAAC TGGGAGCTCT CAGTAAAATT GCATGATAAA 
GTTCATACCG TAGTAGCATC AAACAATGGG TCAGTGTTCT CGGTGGAAGT TGATGGGTCG 
AAACTAAATG TGACCAGCAC GTGGAACCTG GCTTCGCCCT TATTGTCTGT CAGCGTTGAT 
GGCACTCAGA GGACTGTCCA GTGTCTTTCT CGAGAAGCAG GTGGAAACAT GAGCATTCAG 
TTTCTTGGTA CAGTGTACAA GGTGAATATC TTAACCAGAC TTGCCGCAGA ATTGAACAAA 
TTTATGCTGG AAAAAGTGAC TGAGGACACA AGCAGTGTTC TGCGTTCCCC GATGCCCGGA 
GTGGTGGTGG CCGTCTCTGT CAAGCCTGGA GACGCGGTAG CAGAAGGTCA AGAAATTTGT 
GTGATTGAAG CCATGAAAAT GCAGAATAGT ATGACAGCTG GGAAAACTGG CACGGTGAAA 
TCTGTGCACT GTCAAGCTGG AGACACAGTT GGAGAAGGGG ATCTGCTCGT GGAGCTGGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339535.1
CDS	107..2149
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352606.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352606.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGACCC CTACAAGTCT 
TTTGGTTTAC CATCTATTGG GAGATTGTCT CAGTACCAAG AACCGTTACA TCTACCTGGT 
GTCCGAGTGG ACAGTGGCAT CCAACCAGGA AGTGATATTA GCATTTATTA TGATCCTATG 
ATTTCAAAAC TAATCACATA TGGCTCTGAT AGAACTGAGG CACTGAAGAG AATGGCAGAT 
GCACTGGATA ACTATGTTAT TCGAGGTGTT ACACATAATA TTGCATTACT TCGAGAGGTG 
ATAATCAACT CACGCTTTGT AAAAGGAGAC ATCAGCACTA AATTTCTCTC CGATGTGTAT 
CCTGATGGCT TCAAAGGACA CATGCTAACC AAGAGTGAGA AGAACCAGTT ATTGGCAATA 
GCATCATCAT TGTTTGTGGC ATTCCAGTTA AGAGCACAAC ATTTTCAAGA AAATTCAAGA 
ATGCCTGTTA TTAAACCAGA CATAGCCAAC TGGGAGCTCT CAGTAAAATT GCATGATAAA 
GTTCATACCG TAGTAGCATC AAACAATGGG TCAGTGTTCT CGGTGGAAGT TGATGGGTCG 
AAACTAAATG TGACCAGCAC GTGGAACCTG GCTTCGCCCT TATTGTCTGT CAGCGTTGAT 
GGCACTCAGA GGACTGTCCA GTGTCTTTCT CGAGAAGCAG GTGGAAACAT GAGCATTCAG 
TTTCTTGGTA CAGTGTACAA GGTGAATATC TTAACCAGAC TTGCCGCAGA ATTGAACAAA 
TTTATGCTGG AAAAAGTGAC TGAGGACACA AGCAGTGTTC TGCGTTCCCC GATGCCCGGA 
GTGGTGGTGG CCGTCTCTGT CAAGCCTGGA GACGCGGTAG CAGAAGGTCA AGAAATTTGT 
GTGATTGAAG CCATGAAAAT GCAGAATAGT ATGACAGCTG GGAAAACTGG CACGGTGAAA 
TCTGTGCACT GTCAAGCTGG AGACACAGTT GGAGAAGGGG ATCTGCTCGT GGAGCTGGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352608.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339537.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu102909
Clone ID Related Accession (Same CDS sequence)	NM_001352608.2 , NM_001352608.1
Accession Version	NM_001352608.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. On Jun 1, 2019 this sequence version replaced NM_001352608.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.5924.1, SRR1803612.269480.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGAC CCCTACAAGT CTTTTGGTTT ACCATCTATT 
GGGAGATTGT CTCAGTACCA AGAACCGTTA CATCTACCTG GTGTCCGAGT GGACAGTGGC 
ATCCAACCAG GAAGTGATAT TAGCATTTAT TATGATCCTA TGATTTCAAA ACTAATCACA 
TATGGCTCTG ATAGAACTGA GGCACTGAAG AGAATGGCAG ATGCACTGGA TAACTATGTT 
ATTCGAGGTG TTACACATAA TATTGCATTA CTTCGAGAGG TGATAATCAA CTCACGCTTT 
GTAAAAGGAG ACATCAGCAC TAAATTTCTC TCCGATGTGT ATCCTGATGG CTTCAAAGGA 
CACATGCTAA CCAAGAGTGA GAAGAACCAG TTATTGGCAA TAGCATCATC ATTGTTTGTG 
GCATTCCAGT TAAGAGCACA ACATTTTCAA GAAAATTCAA GAATGCCTGT TATTAAACCA 
GACATAGCCA ACTGGGAGCT CTCAGTAAAA TTGCATGATA AAGTTCATAC CGTAGTAGCA 
TCAAACAATG GGTCAGTGTT CTCGGTGGAA GTTGATGGGT CGAAACTAAA TGTGACCAGC 
ACGTGGAACC TGGCTTCGCC CTTATTGTCT GTCAGCGTTG ATGGCACTCA GAGGACTGTC 
CAGTGTCTTT CTCGAGAAGC AGGTGGAAAC ATGAGCATTC AGTTTCTTGG TACAGTGTAC 
AAGGTGAATA TCTTAACCAG ACTTGCCGCA GAATTGAACA AATTTATGCT GGAAAAAGTG 
ACTGAGGACA CAAGCAGTGT TCTGCGTTCC CCGATGCCCG GAGTGGTGGT GGCCGTCTCT 
GTCAAGCCTG GAGACGCGGT AGCAGAAGGT CAAGAAATTT GTGTGATTGA AGCCATGAAA 
ATGCAGAATA GTATGACAGC TGGGAAAACT GGCACGGTGA AATCTGTGCA CTGTCAAGCT 
GGAGACACAG TTGGAGAAGG GGATCTGCTC GTGGAGCTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339537.1
CDS	29..1993
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352608.2
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352608.2

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGAC CCCTACAAGT CTTTTGGTTT ACCATCTATT 
GGGAGATTGT CTCAGTACCA AGAACCGTTA CATCTACCTG GTGTCCGAGT GGACAGTGGC 
ATCCAACCAG GAAGTGATAT TAGCATTTAT TATGATCCTA TGATTTCAAA ACTAATCACA 
TATGGCTCTG ATAGAACTGA GGCACTGAAG AGAATGGCAG ATGCACTGGA TAACTATGTT 
ATTCGAGGTG TTACACATAA TATTGCATTA CTTCGAGAGG TGATAATCAA CTCACGCTTT 
GTAAAAGGAG ACATCAGCAC TAAATTTCTC TCCGATGTGT ATCCTGATGG CTTCAAAGGA 
CACATGCTAA CCAAGAGTGA GAAGAACCAG TTATTGGCAA TAGCATCATC ATTGTTTGTG 
GCATTCCAGT TAAGAGCACA ACATTTTCAA GAAAATTCAA GAATGCCTGT TATTAAACCA 
GACATAGCCA ACTGGGAGCT CTCAGTAAAA TTGCATGATA AAGTTCATAC CGTAGTAGCA 
TCAAACAATG GGTCAGTGTT CTCGGTGGAA GTTGATGGGT CGAAACTAAA TGTGACCAGC 
ACGTGGAACC TGGCTTCGCC CTTATTGTCT GTCAGCGTTG ATGGCACTCA GAGGACTGTC 
CAGTGTCTTT CTCGAGAAGC AGGTGGAAAC ATGAGCATTC AGTTTCTTGG TACAGTGTAC 
AAGGTGAATA TCTTAACCAG ACTTGCCGCA GAATTGAACA AATTTATGCT GGAAAAAGTG 
ACTGAGGACA CAAGCAGTGT TCTGCGTTCC CCGATGCCCG GAGTGGTGGT GGCCGTCTCT 
GTCAAGCCTG GAGACGCGGT AGCAGAAGGT CAAGAAATTT GTGTGATTGA AGCCATGAAA 
ATGCAGAATA GTATGACAGC TGGGAAAACT GGCACGGTGA AATCTGTGCA CTGTCAAGCT 
GGAGACACAG TTGGAGAAGG GGATCTGCTC GTGGAGCTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352608.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339537.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu102909
Clone ID Related Accession (Same CDS sequence)	NM_001352608.2 , NM_001352608.1
Accession Version	NM_001352608.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.269480.1, SRR1803613.5924.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGAC CCCTACAAGT CTTTTGGTTT ACCATCTATT 
GGGAGATTGT CTCAGTACCA AGAACCGTTA CATCTACCTG GTGTCCGAGT GGACAGTGGC 
ATCCAACCAG GAAGTGATAT TAGCATTTAT TATGATCCTA TGATTTCAAA ACTAATCACA 
TATGGCTCTG ATAGAACTGA GGCACTGAAG AGAATGGCAG ATGCACTGGA TAACTATGTT 
ATTCGAGGTG TTACACATAA TATTGCATTA CTTCGAGAGG TGATAATCAA CTCACGCTTT 
GTAAAAGGAG ACATCAGCAC TAAATTTCTC TCCGATGTGT ATCCTGATGG CTTCAAAGGA 
CACATGCTAA CCAAGAGTGA GAAGAACCAG TTATTGGCAA TAGCATCATC ATTGTTTGTG 
GCATTCCAGT TAAGAGCACA ACATTTTCAA GAAAATTCAA GAATGCCTGT TATTAAACCA 
GACATAGCCA ACTGGGAGCT CTCAGTAAAA TTGCATGATA AAGTTCATAC CGTAGTAGCA 
TCAAACAATG GGTCAGTGTT CTCGGTGGAA GTTGATGGGT CGAAACTAAA TGTGACCAGC 
ACGTGGAACC TGGCTTCGCC CTTATTGTCT GTCAGCGTTG ATGGCACTCA GAGGACTGTC 
CAGTGTCTTT CTCGAGAAGC AGGTGGAAAC ATGAGCATTC AGTTTCTTGG TACAGTGTAC 
AAGGTGAATA TCTTAACCAG ACTTGCCGCA GAATTGAACA AATTTATGCT GGAAAAAGTG 
ACTGAGGACA CAAGCAGTGT TCTGCGTTCC CCGATGCCCG GAGTGGTGGT GGCCGTCTCT 
GTCAAGCCTG GAGACGCGGT AGCAGAAGGT CAAGAAATTT GTGTGATTGA AGCCATGAAA 
ATGCAGAATA GTATGACAGC TGGGAAAACT GGCACGGTGA AATCTGTGCA CTGTCAAGCT 
GGAGACACAG TTGGAGAAGG GGATCTGCTC GTGGAGCTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339537.1
CDS	107..2071
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352608.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352608.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGAC CCCTACAAGT CTTTTGGTTT ACCATCTATT 
GGGAGATTGT CTCAGTACCA AGAACCGTTA CATCTACCTG GTGTCCGAGT GGACAGTGGC 
ATCCAACCAG GAAGTGATAT TAGCATTTAT TATGATCCTA TGATTTCAAA ACTAATCACA 
TATGGCTCTG ATAGAACTGA GGCACTGAAG AGAATGGCAG ATGCACTGGA TAACTATGTT 
ATTCGAGGTG TTACACATAA TATTGCATTA CTTCGAGAGG TGATAATCAA CTCACGCTTT 
GTAAAAGGAG ACATCAGCAC TAAATTTCTC TCCGATGTGT ATCCTGATGG CTTCAAAGGA 
CACATGCTAA CCAAGAGTGA GAAGAACCAG TTATTGGCAA TAGCATCATC ATTGTTTGTG 
GCATTCCAGT TAAGAGCACA ACATTTTCAA GAAAATTCAA GAATGCCTGT TATTAAACCA 
GACATAGCCA ACTGGGAGCT CTCAGTAAAA TTGCATGATA AAGTTCATAC CGTAGTAGCA 
TCAAACAATG GGTCAGTGTT CTCGGTGGAA GTTGATGGGT CGAAACTAAA TGTGACCAGC 
ACGTGGAACC TGGCTTCGCC CTTATTGTCT GTCAGCGTTG ATGGCACTCA GAGGACTGTC 
CAGTGTCTTT CTCGAGAAGC AGGTGGAAAC ATGAGCATTC AGTTTCTTGG TACAGTGTAC 
AAGGTGAATA TCTTAACCAG ACTTGCCGCA GAATTGAACA AATTTATGCT GGAAAAAGTG 
ACTGAGGACA CAAGCAGTGT TCTGCGTTCC CCGATGCCCG GAGTGGTGGT GGCCGTCTCT 
GTCAAGCCTG GAGACGCGGT AGCAGAAGGT CAAGAAATTT GTGTGATTGA AGCCATGAAA 
ATGCAGAATA GTATGACAGC TGGGAAAACT GGCACGGTGA AATCTGTGCA CTGTCAAGCT 
GGAGACACAG TTGGAGAAGG GGATCTGCTC GTGGAGCTGG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352605.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339534.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu84557
Clone ID Related Accession (Same CDS sequence)	NM_001352605.1 , NM_001352605.2 , XM_017020608.1
Accession Version	NM_001352605.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform d precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. On Jun 18, 2017 this sequence version replaced XM_017020608.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.164351.1, SRR1803612.58708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTACAA GGTGAATATC 
TTAACCAGAC TTGCCGCAGA ATTGAACAAA TTTATGCTGG AAAAAGTGAC TGAGGACACA 
AGCAGTGTTC TGCGTTCCCC GATGCCCGGA GTGGTGGTGG CCGTCTCTGT CAAGCCTGGA 
GACGCGGTAG CAGAAGGTCA AGAAATTTGT GTGATTGAAG CCATGAAAAT GCAGAATAGT 
ATGACAGCTG GGAAAACTGG CACGGTGAAA TCTGTGCACT GTCAAGCTGG AGACACAGTT 
GGAGAAGGGG ATCTGCTCGT GGAGCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339534.1
CDS	107..2239
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352605.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352605.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTACAA GGTGAATATC 
TTAACCAGAC TTGCCGCAGA ATTGAACAAA TTTATGCTGG AAAAAGTGAC TGAGGACACA 
AGCAGTGTTC TGCGTTCCCC GATGCCCGGA GTGGTGGTGG CCGTCTCTGT CAAGCCTGGA 
GACGCGGTAG CAGAAGGTCA AGAAATTTGT GTGATTGAAG CCATGAAAAT GCAGAATAGT 
ATGACAGCTG GGAAAACTGG CACGGTGAAA TCTGTGCACT GTCAAGCTGG AGACACAGTT 
GGAGAAGGGG ATCTGCTCGT GGAGCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352605.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339534.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu84557
Clone ID Related Accession (Same CDS sequence)	NM_001352605.1 , NM_001352605.2 , XM_017020608.1
Accession Version	NM_001352605.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform d precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. On May 31, 2019 this sequence version replaced NM_001352605.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.164351.1, SRR1803612.58708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTACAA GGTGAATATC 
TTAACCAGAC TTGCCGCAGA ATTGAACAAA TTTATGCTGG AAAAAGTGAC TGAGGACACA 
AGCAGTGTTC TGCGTTCCCC GATGCCCGGA GTGGTGGTGG CCGTCTCTGT CAAGCCTGGA 
GACGCGGTAG CAGAAGGTCA AGAAATTTGT GTGATTGAAG CCATGAAAAT GCAGAATAGT 
ATGACAGCTG GGAAAACTGG CACGGTGAAA TCTGTGCACT GTCAAGCTGG AGACACAGTT 
GGAGAAGGGG ATCTGCTCGT GGAGCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339534.1
CDS	29..2161
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352605.2
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352605.2

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTACAA GGTGAATATC 
TTAACCAGAC TTGCCGCAGA ATTGAACAAA TTTATGCTGG AAAAAGTGAC TGAGGACACA 
AGCAGTGTTC TGCGTTCCCC GATGCCCGGA GTGGTGGTGG CCGTCTCTGT CAAGCCTGGA 
GACGCGGTAG CAGAAGGTCA AGAAATTTGT GTGATTGAAG CCATGAAAAT GCAGAATAGT 
ATGACAGCTG GGAAAACTGG CACGGTGAAA TCTGTGCACT GTCAAGCTGG AGACACAGTT 
GGAGAAGGGG ATCTGCTCGT GGAGCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352607.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339536.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu84563
Clone ID Related Accession (Same CDS sequence)	NM_001352607.1 , NM_001352607.2 , XM_017020614.1
Accession Version	NM_001352607.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. On Jun 18, 2017 this sequence version replaced XM_017020614.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.169017.1, SRR1803614.170565.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGTT GAGCATCCTG TCACAGAATG CATTACTGGC 
CTGGACCTAG TCCAGGAAAT GATCCGTGTT GCTAAGGGCT ACCCTCTCAG GCACAAACAA 
GCTGATATTC GCATCAACGG CTGGGCAGTT GAATGTCGGG TTTATGCTGA GGACCCCTAC 
AAGTCTTTTG GTTTACCATC TATTGGGAGA TTGTCTCAGT ACCAAGAACC GTTACATCTA 
CCTGGTGTCC GAGTGGACAG TGGCATCCAA CCAGGAAGTG ATATTAGCAT TTATTATGAT 
CCTATGATTT CAAAACTAAT CACATATGGC TCTGATAGAA CTGAGGCACT GAAGAGAATG 
GCAGATGCAC TGGATAACTA TGTTATTCGA GGTGTTACAC ATAATATTGC ATTACTTCGA 
GAGGTGATAA TCAACTCACG CTTTGTAAAA GGAGACATCA GCACTAAATT TCTCTCCGAT 
GTGTATCCTG ATGGCTTCAA AGGACACATG CTAACCAAGA GTGAGAAGAA CCAGTTATTG 
GCAATAGCAT CATCATTGTT TGTGGCATTC CAGTTAAGAG CACAACATTT TCAAGAAAAT 
TCAAGAATGC CTGTTATTAA ACCAGACATA GCCAACTGGG AGCTCTCAGT AAAATTGCAT 
GATAAAGTTC ATACCGTAGT AGCATCAAAC AATGGGTCAG TGTTCTCGGT GGAAGTTGAT 
GGGTCGAAAC TAAATGTGAC CAGCACGTGG AACCTGGCTT CGCCCTTATT GTCTGTCAGC 
GTTGATGGCA CTCAGAGGAC TGTCCAGTGT CTTTCTCGAG AAGCAGGTGG AAACATGAGC 
ATTCAGTTTC TTGGTACAGT GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339536.1
CDS	107..2074
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352607.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352607.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGTT GAGCATCCTG TCACAGAATG CATTACTGGC 
CTGGACCTAG TCCAGGAAAT GATCCGTGTT GCTAAGGGCT ACCCTCTCAG GCACAAACAA 
GCTGATATTC GCATCAACGG CTGGGCAGTT GAATGTCGGG TTTATGCTGA GGACCCCTAC 
AAGTCTTTTG GTTTACCATC TATTGGGAGA TTGTCTCAGT ACCAAGAACC GTTACATCTA 
CCTGGTGTCC GAGTGGACAG TGGCATCCAA CCAGGAAGTG ATATTAGCAT TTATTATGAT 
CCTATGATTT CAAAACTAAT CACATATGGC TCTGATAGAA CTGAGGCACT GAAGAGAATG 
GCAGATGCAC TGGATAACTA TGTTATTCGA GGTGTTACAC ATAATATTGC ATTACTTCGA 
GAGGTGATAA TCAACTCACG CTTTGTAAAA GGAGACATCA GCACTAAATT TCTCTCCGAT 
GTGTATCCTG ATGGCTTCAA AGGACACATG CTAACCAAGA GTGAGAAGAA CCAGTTATTG 
GCAATAGCAT CATCATTGTT TGTGGCATTC CAGTTAAGAG CACAACATTT TCAAGAAAAT 
TCAAGAATGC CTGTTATTAA ACCAGACATA GCCAACTGGG AGCTCTCAGT AAAATTGCAT 
GATAAAGTTC ATACCGTAGT AGCATCAAAC AATGGGTCAG TGTTCTCGGT GGAAGTTGAT 
GGGTCGAAAC TAAATGTGAC CAGCACGTGG AACCTGGCTT CGCCCTTATT GTCTGTCAGC 
GTTGATGGCA CTCAGAGGAC TGTCCAGTGT CTTTCTCGAG AAGCAGGTGG AAACATGAGC 
ATTCAGTTTC TTGGTACAGT GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( NM_001352607.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339536.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu84563
Clone ID Related Accession (Same CDS sequence)	NM_001352607.1 , NM_001352607.2 , XM_017020614.1
Accession Version	NM_001352607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL355338.33, AL356575.8, AL353697.21 and AL136526.27. On May 31, 2019 this sequence version replaced NM_001352607.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.514847.1, SRR1803614.171617.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGTT GAGCATCCTG TCACAGAATG CATTACTGGC 
CTGGACCTAG TCCAGGAAAT GATCCGTGTT GCTAAGGGCT ACCCTCTCAG GCACAAACAA 
GCTGATATTC GCATCAACGG CTGGGCAGTT GAATGTCGGG TTTATGCTGA GGACCCCTAC 
AAGTCTTTTG GTTTACCATC TATTGGGAGA TTGTCTCAGT ACCAAGAACC GTTACATCTA 
CCTGGTGTCC GAGTGGACAG TGGCATCCAA CCAGGAAGTG ATATTAGCAT TTATTATGAT 
CCTATGATTT CAAAACTAAT CACATATGGC TCTGATAGAA CTGAGGCACT GAAGAGAATG 
GCAGATGCAC TGGATAACTA TGTTATTCGA GGTGTTACAC ATAATATTGC ATTACTTCGA 
GAGGTGATAA TCAACTCACG CTTTGTAAAA GGAGACATCA GCACTAAATT TCTCTCCGAT 
GTGTATCCTG ATGGCTTCAA AGGACACATG CTAACCAAGA GTGAGAAGAA CCAGTTATTG 
GCAATAGCAT CATCATTGTT TGTGGCATTC CAGTTAAGAG CACAACATTT TCAAGAAAAT 
TCAAGAATGC CTGTTATTAA ACCAGACATA GCCAACTGGG AGCTCTCAGT AAAATTGCAT 
GATAAAGTTC ATACCGTAGT AGCATCAAAC AATGGGTCAG TGTTCTCGGT GGAAGTTGAT 
GGGTCGAAAC TAAATGTGAC CAGCACGTGG AACCTGGCTT CGCCCTTATT GTCTGTCAGC 
GTTGATGGCA CTCAGAGGAC TGTCCAGTGT CTTTCTCGAG AAGCAGGTGG AAACATGAGC 
ATTCAGTTTC TTGGTACAGT GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339536.1
CDS	29..1996
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	NM_001352607.2
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001352607.2

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGTT GAGCATCCTG TCACAGAATG CATTACTGGC 
CTGGACCTAG TCCAGGAAAT GATCCGTGTT GCTAAGGGCT ACCCTCTCAG GCACAAACAA 
GCTGATATTC GCATCAACGG CTGGGCAGTT GAATGTCGGG TTTATGCTGA GGACCCCTAC 
AAGTCTTTTG GTTTACCATC TATTGGGAGA TTGTCTCAGT ACCAAGAACC GTTACATCTA 
CCTGGTGTCC GAGTGGACAG TGGCATCCAA CCAGGAAGTG ATATTAGCAT TTATTATGAT 
CCTATGATTT CAAAACTAAT CACATATGGC TCTGATAGAA CTGAGGCACT GAAGAGAATG 
GCAGATGCAC TGGATAACTA TGTTATTCGA GGTGTTACAC ATAATATTGC ATTACTTCGA 
GAGGTGATAA TCAACTCACG CTTTGTAAAA GGAGACATCA GCACTAAATT TCTCTCCGAT 
GTGTATCCTG ATGGCTTCAA AGGACACATG CTAACCAAGA GTGAGAAGAA CCAGTTATTG 
GCAATAGCAT CATCATTGTT TGTGGCATTC CAGTTAAGAG CACAACATTT TCAAGAAAAT 
TCAAGAATGC CTGTTATTAA ACCAGACATA GCCAACTGGG AGCTCTCAGT AAAATTGCAT 
GATAAAGTTC ATACCGTAGT AGCATCAAAC AATGGGTCAG TGTTCTCGGT GGAAGTTGAT 
GGGTCGAAAC TAAATGTGAC CAGCACGTGG AACCTGGCTT CGCCCTTATT GTCTGTCAGC 
GTTGATGGCA CTCAGAGGAC TGTCCAGTGT CTTTCTCGAG AAGCAGGTGG AAACATGAGC 
ATTCAGTTTC TTGGTACAGT GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020605.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876094.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu84554
Clone ID Related Accession (Same CDS sequence)	XM_017020605.1
Accession Version	XM_017020605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGGAGGT TCTCCAGGTC 
ATGTGCCGGT ATAACAGCCA TGCTGTGGGG CCTGTGCTTT CTCATTCCTG CATGGCAAGG 
CCAGTGTCTT TCTCGAGAAG CAGGTGGAAA CATGAGCATT CAGTTTCTTG GTACAGTGGA 
GCACGGGGCT CAGGGAAAGT TCAGCATTGT CAGTTGTCAA CAAAATCAGT AAGTGTTATT 
AAATACCCCA TGGTGTGTGG TTCTGGCACT GAAATGGGGC TGTTTCCTAA GGCTCTGTCC 
ATGGCCACCT CTGTCTATTT CTGCATTCTG TCTTGCATTG CTTCTGTTGC CTATATCCTG 
GTCAGCACAG TTTATGGTGA ATCATTGCTT ATTGAAATAG AGACGGCAAT AATGAAGCCA 
GCTATTATAA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876094.1
CDS	102..2336
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQEVLQVMCRYNSHAVGPVLSHSCMARPVSFSRSRWKHEHSVSWYSGARGSGKVQHCQLSTKSVSVIKYPMVCGSGTEMGLFPKALSMATSVYFCILSCIASVAYILVSTVYGESLLIEIETAIMKPAIIN

Target ORF information:

RefSeq Version	XM_017020605.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020605.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGGAGGT TCTCCAGGTC 
ATGTGCCGGT ATAACAGCCA TGCTGTGGGG CCTGTGCTTT CTCATTCCTG CATGGCAAGG 
CCAGTGTCTT TCTCGAGAAG CAGGTGGAAA CATGAGCATT CAGTTTCTTG GTACAGTGGA 
GCACGGGGCT CAGGGAAAGT TCAGCATTGT CAGTTGTCAA CAAAATCAGT AAGTGTTATT 
AAATACCCCA TGGTGTGTGG TTCTGGCACT GAAATGGGGC TGTTTCCTAA GGCTCTGTCC 
ATGGCCACCT CTGTCTATTT CTGCATTCTG TCTTGCATTG CTTCTGTTGC CTATATCCTG 
GTCAGCACAG TTTATGGTGA ATCATTGCTT ATTGAAATAG AGACGGCAAT AATGAAGCCA 
GCTATTATAA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020606.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876095.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu84555
Clone ID Related Accession (Same CDS sequence)	XM_017020606.1
Accession Version	XM_017020606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGTT GAGCATCCTG TCACAGAATG CATTACTGGC 
CTGGACCTAG TCCAGGAAAT GATCCGTGTT GCTAAGGGCT ACCCTCTCAG GCACAAACAA 
GCTGATATTC GCATCAACGG CTGGGCAGTT GAATGTCGGG TTTATGCTGA GGACCCCTAC 
AAGTCTTTTG GTTTACCATC TATTGGGAGA TTGTCTCAGT ACCAAGAACC GTTACATCTA 
CCTGGTGTCC GAGTGGACAG TGGCATCCAA CCAGGAAGTG ATATTAGCAT TTATTATGAT 
CCTATGATTT CAAAACTAAT CACATATGGC TCTGATAGAA CTGAGGCACT GAAGAGAATG 
GCAGATGCAC TGGATAACTA TGTTATTCGA GGTGTTACAC ATAATATTGC ATTACTTCGA 
GAGGTGATAA TCAACTCACG CTTTGTAAAA GGAGACATCA GCACTAAATT TCTCTCCGAT 
GTGTATCCTG ATGGCTTCAA AGGACACATG CTAACCAAGA GTGAGAAGAA CCAGTTATTG 
GCAATAGCAT CATCATTGTT TGTGGCATTC CAGTTAAGAG CACAACATTT TCAAGAAAAT 
TCAAGAATGC CTGTTATTAA ACCAGACATA GCCAACTGGG AGCTCTCAGT AAAATTGCAT 
GATAAAGTTC ATACCGTAGT AGCATCAAAC AATGGGTCAG TGTTCTCGGT GGAAGTTGAT 
GGGTCGAAAC TAAATGTGAC CAGCACGTGG AACCTGGCTT CGCCCTTATT GTCTGTCAGC 
GTTGATGGCA CTCAGAGGAC TGTCCAGGAG GTTCTCCAGG TCATGTGCCG GTATAACAGC 
CATGCTGTGG GGCCTGTGCT TTCTCATTCC TGCATGGCAA GGCCAGTGTC TTTCTCGAGA 
AGCAGGTGGA AACATGAGCA TTCAGTTTCT TGGTACAGTG GAGCACGGGG CTCAGGGAAA 
GTTCAGCATT GTCAGTTGTC AACAAAATCA GTAAGTGTTA TTAAATACCC CATGGTGTGT 
GGTTCTGGCA CTGAAATGGG GCTGTTTCCT AAGGCTCTGT CCATGGCCAC CTCTGTCTAT 
TTCTGCATTC TGTCTTGCAT TGCTTCTGTT GCCTATATCC TGGTCAGCAC AGTTTATGGT 
GAATCATTGC TTATTGAAAT AGAGACGGCA ATAATGAAGC CAGCTATTAT AAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876095.1
CDS	31..2187
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQEVLQVMCRYNSHAVGPVLSHSCMARPVSFSRSRWKHEHSVSWYSGARGSGKVQHCQLSTKSVSVIKYPMVCGSGTEMGLFPKALSMATSVYFCILSCIASVAYILVSTVYGESLLIEIETAIMKPAIIN

Target ORF information:

RefSeq Version	XM_017020606.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020606.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGTT GAGCATCCTG TCACAGAATG CATTACTGGC 
CTGGACCTAG TCCAGGAAAT GATCCGTGTT GCTAAGGGCT ACCCTCTCAG GCACAAACAA 
GCTGATATTC GCATCAACGG CTGGGCAGTT GAATGTCGGG TTTATGCTGA GGACCCCTAC 
AAGTCTTTTG GTTTACCATC TATTGGGAGA TTGTCTCAGT ACCAAGAACC GTTACATCTA 
CCTGGTGTCC GAGTGGACAG TGGCATCCAA CCAGGAAGTG ATATTAGCAT TTATTATGAT 
CCTATGATTT CAAAACTAAT CACATATGGC TCTGATAGAA CTGAGGCACT GAAGAGAATG 
GCAGATGCAC TGGATAACTA TGTTATTCGA GGTGTTACAC ATAATATTGC ATTACTTCGA 
GAGGTGATAA TCAACTCACG CTTTGTAAAA GGAGACATCA GCACTAAATT TCTCTCCGAT 
GTGTATCCTG ATGGCTTCAA AGGACACATG CTAACCAAGA GTGAGAAGAA CCAGTTATTG 
GCAATAGCAT CATCATTGTT TGTGGCATTC CAGTTAAGAG CACAACATTT TCAAGAAAAT 
TCAAGAATGC CTGTTATTAA ACCAGACATA GCCAACTGGG AGCTCTCAGT AAAATTGCAT 
GATAAAGTTC ATACCGTAGT AGCATCAAAC AATGGGTCAG TGTTCTCGGT GGAAGTTGAT 
GGGTCGAAAC TAAATGTGAC CAGCACGTGG AACCTGGCTT CGCCCTTATT GTCTGTCAGC 
GTTGATGGCA CTCAGAGGAC TGTCCAGGAG GTTCTCCAGG TCATGTGCCG GTATAACAGC 
CATGCTGTGG GGCCTGTGCT TTCTCATTCC TGCATGGCAA GGCCAGTGTC TTTCTCGAGA 
AGCAGGTGGA AACATGAGCA TTCAGTTTCT TGGTACAGTG GAGCACGGGG CTCAGGGAAA 
GTTCAGCATT GTCAGTTGTC AACAAAATCA GTAAGTGTTA TTAAATACCC CATGGTGTGT 
GGTTCTGGCA CTGAAATGGG GCTGTTTCCT AAGGCTCTGT CCATGGCCAC CTCTGTCTAT 
TTCTGCATTC TGTCTTGCAT TGCTTCTGTT GCCTATATCC TGGTCAGCAC AGTTTATGGT 
GAATCATTGC TTATTGAAAT AGAGACGGCA ATAATGAAGC CAGCTATTAT AAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020607.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876096.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu84556
Clone ID Related Accession (Same CDS sequence)	XM_017020607.1
Accession Version	XM_017020607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACATG TTCTGTACTA TTCAAGACAG TGCTTAATGG TGTCCCGTAA TCTTGGTTCA 
GTGGGATATG ATCCTAATGA AAAAACTTTT GATAAAATTC TTGTTGCTAA TAGAGGAGAA 
ATTGCATGTC GGGTTATTAG AACTTGCAAG AAGATGGGCA TTAAGACAGT TGCCATCCAC 
AGTGATGTTG ATGCTAGTTC TGTTCATGTG AAAATGGCGG ATGAGGCTGT CTGTGTTGGC 
CCAGCTCCCA CCAGTAAAAG CTACCTCAAC ATGGATGCCA TCATGGAAGC CATTAAGAAA 
ACCAGGGCCC AAGCTGTACA TCCAGGTTAT GGATTCCTTT CAGAAAACAA AGAATTTGCC 
AGATGTTTGG CAGCAGAAGA TGTCGTTTTC ATTGGACCTG ACACACATGC TATTCAAGCC 
ATGGGCGACA AGATTGAAAG CAAATTATTA GCTAAGAAAG CAGAGGTTAA TACAATCCCT 
GGCTTTGATG GAGTAGTCAA GGATGCAGAA GAAGCTGTCA GAATTGCAAG GGAAATTGGC 
TACCCTGTCA TGATCAAGGC CTCAGCAGGT GGTGGTGGGA AAGGCATGCG CATTGCTTGG 
GATGATGAAG AGACCAGGGA TGGTTTTAGA TTGTCATCTC AAGAAGCTGC TTCTAGTTTT 
GGCGATGATA GACTACTAAT AGAAAAATTT ATTGATAATC CTCGTCATAT AGAAATCCAG 
GTTCTAGGTG ATAAACATGG GAATGCTTTA TGGCTTAATG AAAGAGAGTG CTCAATTCAG 
AGAAGAAATC AGAAGGTGGT GGAGGAAGCA CCAAGCATTT TTTTGGATGC GGAGACTCGA 
AGAGCGATGG GAGAACAAGC TGTAGCTCTT GCCAGAGCAG TAAAATATTC CTCTGCTGGG 
ACCGTGGAGT TCCTTGTGGA CTCTAAGAAG AATTTTTATT TCTTGGAAAT GAATACAAGA 
CTCCAGGTTG AGCATCCTGT CACAGAATGC ATTACTGGCC TGGACCTAGT CCAGGAAATG 
ATCCGTGTTG CTAAGGGCTA CCCTCTCAGG CACAAACAAG CTGATATTCG CATCAACGGC 
TGGGCAGTTG AATGTCGGGT TTATGCTGAG GACCCCTACA AGTCTTTTGG TTTACCATCT 
ATTGGGAGAT TGTCTCAGTA CCAAGAACCG TTACATCTAC CTGGTGTCCG AGTGGACAGT 
GGCATCCAAC CAGGAAGTGA TATTAGCATT TATTATGATC CTATGATTTC AAAACTAATC 
ACATATGGCT CTGATAGAAC TGAGGCACTG AAGAGAATGG CAGATGCACT GGATAACTAT 
GTTATTCGAG GTGTTACACA TAATATTGCA TTACTTCGAG AGGTGATAAT CAACTCACGC 
TTTGTAAAAG GAGACATCAG CACTAAATTT CTCTCCGATG TGTATCCTGA TGGCTTCAAA 
GGACACATGC TAACCAAGAG TGAGAAGAAC CAGTTATTGG CAATAGCATC ATCATTGTTT 
GTGGCATTCC AGTTAAGAGC ACAACATTTT CAAGAAAATT CAAGAATGCC TGTTATTAAA 
CCAGACATAG CCAACTGGGA GCTCTCAGTA AAATTGCATG ATAAAGTTCA TACCGTAGTA 
GCATCAAACA ATGGGTCAGT GTTCTCGGTG GAAGTTGATG GGTCGAAACT AAATGTGACC 
AGCACGTGGA ACCTGGCTTC GCCCTTATTG TCTGTCAGCG TTGATGGCAC TCAGAGGACT 
GTCCAGGAGG TTCTCCAGGT CATGTGCCGG TATAACAGCC ATGCTGTGGG GCCTGTGCTT 
TCTCATTCCT GCATGGCAAG GCCAGTGTCT TTCTCGAGAA GCAGGTGGAA ACATGAGCAT 
TCAGTTTCTT GGTACAGTGG AGCACGGGGC TCAGGGAAAG TTCAGCATTG TCAGTTGTCA 
ACAAAATCAG TAAGTGTTAT TAAATACCCC ATGGTGTGTG GTTCTGGCAC TGAAATGGGG 
CTGTTTCCTA AGGCTCTGTC CATGGCCACC TCTGTCTATT TCTGCATTCT GTCTTGCATT 
GCTTCTGTTG CCTATATCCT GGTCAGCACA GTTTATGGTG AATCATTGCT TATTGAAATA 
GAGACGGCAA TAATGAAGCC AGCTATTATA AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876096.1
CDS	17..2152
Translation	MEHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQEVLQVMCRYNSHAVGPVLSHSCMARPVSFSRSRWKHEHSVSWYSGARGSGKVQHCQLSTKSVSVIKYPMVCGSGTEMGLFPKALSMATSVYFCILSCIASVAYILVSTVYGESLLIEIETAIMKPAIIN

Target ORF information:

RefSeq Version	XM_017020607.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020607.1

ORF Insert Sequence:

ATGGAACATG TTCTGTACTA TTCAAGACAG TGCTTAATGG TGTCCCGTAA TCTTGGTTCA 
GTGGGATATG ATCCTAATGA AAAAACTTTT GATAAAATTC TTGTTGCTAA TAGAGGAGAA 
ATTGCATGTC GGGTTATTAG AACTTGCAAG AAGATGGGCA TTAAGACAGT TGCCATCCAC 
AGTGATGTTG ATGCTAGTTC TGTTCATGTG AAAATGGCGG ATGAGGCTGT CTGTGTTGGC 
CCAGCTCCCA CCAGTAAAAG CTACCTCAAC ATGGATGCCA TCATGGAAGC CATTAAGAAA 
ACCAGGGCCC AAGCTGTACA TCCAGGTTAT GGATTCCTTT CAGAAAACAA AGAATTTGCC 
AGATGTTTGG CAGCAGAAGA TGTCGTTTTC ATTGGACCTG ACACACATGC TATTCAAGCC 
ATGGGCGACA AGATTGAAAG CAAATTATTA GCTAAGAAAG CAGAGGTTAA TACAATCCCT 
GGCTTTGATG GAGTAGTCAA GGATGCAGAA GAAGCTGTCA GAATTGCAAG GGAAATTGGC 
TACCCTGTCA TGATCAAGGC CTCAGCAGGT GGTGGTGGGA AAGGCATGCG CATTGCTTGG 
GATGATGAAG AGACCAGGGA TGGTTTTAGA TTGTCATCTC AAGAAGCTGC TTCTAGTTTT 
GGCGATGATA GACTACTAAT AGAAAAATTT ATTGATAATC CTCGTCATAT AGAAATCCAG 
GTTCTAGGTG ATAAACATGG GAATGCTTTA TGGCTTAATG AAAGAGAGTG CTCAATTCAG 
AGAAGAAATC AGAAGGTGGT GGAGGAAGCA CCAAGCATTT TTTTGGATGC GGAGACTCGA 
AGAGCGATGG GAGAACAAGC TGTAGCTCTT GCCAGAGCAG TAAAATATTC CTCTGCTGGG 
ACCGTGGAGT TCCTTGTGGA CTCTAAGAAG AATTTTTATT TCTTGGAAAT GAATACAAGA 
CTCCAGGTTG AGCATCCTGT CACAGAATGC ATTACTGGCC TGGACCTAGT CCAGGAAATG 
ATCCGTGTTG CTAAGGGCTA CCCTCTCAGG CACAAACAAG CTGATATTCG CATCAACGGC 
TGGGCAGTTG AATGTCGGGT TTATGCTGAG GACCCCTACA AGTCTTTTGG TTTACCATCT 
ATTGGGAGAT TGTCTCAGTA CCAAGAACCG TTACATCTAC CTGGTGTCCG AGTGGACAGT 
GGCATCCAAC CAGGAAGTGA TATTAGCATT TATTATGATC CTATGATTTC AAAACTAATC 
ACATATGGCT CTGATAGAAC TGAGGCACTG AAGAGAATGG CAGATGCACT GGATAACTAT 
GTTATTCGAG GTGTTACACA TAATATTGCA TTACTTCGAG AGGTGATAAT CAACTCACGC 
TTTGTAAAAG GAGACATCAG CACTAAATTT CTCTCCGATG TGTATCCTGA TGGCTTCAAA 
GGACACATGC TAACCAAGAG TGAGAAGAAC CAGTTATTGG CAATAGCATC ATCATTGTTT 
GTGGCATTCC AGTTAAGAGC ACAACATTTT CAAGAAAATT CAAGAATGCC TGTTATTAAA 
CCAGACATAG CCAACTGGGA GCTCTCAGTA AAATTGCATG ATAAAGTTCA TACCGTAGTA 
GCATCAAACA ATGGGTCAGT GTTCTCGGTG GAAGTTGATG GGTCGAAACT AAATGTGACC 
AGCACGTGGA ACCTGGCTTC GCCCTTATTG TCTGTCAGCG TTGATGGCAC TCAGAGGACT 
GTCCAGGAGG TTCTCCAGGT CATGTGCCGG TATAACAGCC ATGCTGTGGG GCCTGTGCTT 
TCTCATTCCT GCATGGCAAG GCCAGTGTCT TTCTCGAGAA GCAGGTGGAA ACATGAGCAT 
TCAGTTTCTT GGTACAGTGG AGCACGGGGC TCAGGGAAAG TTCAGCATTG TCAGTTGTCA 
ACAAAATCAG TAAGTGTTAT TAAATACCCC ATGGTGTGTG GTTCTGGCAC TGAAATGGGG 
CTGTTTCCTA AGGCTCTGTC CATGGCCACC TCTGTCTATT TCTGCATTCT GTCTTGCATT 
GCTTCTGTTG CCTATATCCT GGTCAGCACA GTTTATGGTG AATCATTGCT TATTGAAATA 
GAGACGGCAA TAATGAAGCC AGCTATTATA AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020608.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876097.1 Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu84557
Clone ID Related Accession (Same CDS sequence)	NM_001352605.1 , NM_001352605.2 , XM_017020608.1
Accession Version	XM_017020608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTACAA GGTGAATATC 
TTAACCAGAC TTGCCGCAGA ATTGAACAAA TTTATGCTGG AAAAAGTGAC TGAGGACACA 
AGCAGTGTTC TGCGTTCCCC GATGCCCGGA GTGGTGGTGG CCGTCTCTGT CAAGCCTGGA 
GACGCGGTAG CAGAAGGTCA AGAAATTTGT GTGATTGAAG CCATGAAAAT GCAGAATAGT 
ATGACAGCTG GGAAAACTGG CACGGTGAAA TCTGTGCACT GTCAAGCTGG AGACACAGTT 
GGAGAAGGGG ATCTGCTCGT GGAGCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876097.1
CDS	102..2234
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	XM_017020608.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020608.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGTACAA GGTGAATATC 
TTAACCAGAC TTGCCGCAGA ATTGAACAAA TTTATGCTGG AAAAAGTGAC TGAGGACACA 
AGCAGTGTTC TGCGTTCCCC GATGCCCGGA GTGGTGGTGG CCGTCTCTGT CAAGCCTGGA 
GACGCGGTAG CAGAAGGTCA AGAAATTTGT GTGATTGAAG CCATGAAAAT GCAGAATAGT 
ATGACAGCTG GGAAAACTGG CACGGTGAAA TCTGTGCACT GTCAAGCTGG AGACACAGTT 
GGAGAAGGGG ATCTGCTCGT GGAGCTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020609.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876098.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu84558
Clone ID Related Accession (Same CDS sequence)	XM_017020609.1
Accession Version	XM_017020609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACATG TTCTGTACTA TTCAAGACAG TGCTTAATGG TGTCCCGTAA TCTTGGTTCA 
GTGGGATATG ATCCTAATGA AAAAACTTTT GATAAAATTC TTGTTGCTAA TAGAGGAGAA 
ATTGCATGTC GGGTTATTAG AACTTGCAAG AAGATGGGCA TTAAGACAGT TGCCATCCAC 
AGTGATGTTG ATGCTAGTTC TGTTCATGTG AAAATGGCGG ATGAGGCTGT CTGTGTTGGC 
CCAGCTCCCA CCAGTAAAAG CTACCTCAAC ATGGATGCCA TCATGGAAGC CATTAAGAAA 
ACCAGGGCCC AAGCTGTACA TCCAGGTTAT GGATTCCTTT CAGAAAACAA AGAATTTGCC 
AGATGTTTGG CAGCAGAAGA TGTCGTTTTC ATTGGACCTG ACACACATGC TATTCAAGCC 
ATGGGCGACA AGATTGAAAG CAAATTATTA GCTAAGAAAG CAGAGGTTAA TACAATCCCT 
GGCTTTGATG GAGTAGTCAA GGATGCAGAA GAAGCTGTCA GAATTGCAAG GGAAATTGGC 
TACCCTGTCA TGATCAAGGC CTCAGCAGGT GGTGGTGGGA AAGGCATGCG CATTGCTTGG 
GATGATGAAG AGACCAGGGA TGGTTTTAGA TTGTCATCTC AAGAAGCTGC TTCTAGTTTT 
GGCGATGATA GACTACTAAT AGAAAAATTT ATTGATAATC CTCGTCATAT AGAAATCCAG 
GTTCTAGGTG ATAAACATGG GAATGCTTTA TGGCTTAATG AAAGAGAGTG CTCAATTCAG 
AGAAGAAATC AGAAGGTGGT GGAGGAAGCA CCAAGCATTT TTTTGGATGC GGAGACTCGA 
AGAGCGATGG GAGAACAAGC TGTAGCTCTT GCCAGAGCAG TAAAATATTC CTCTGCTGGG 
ACCGTGGAGT TCCTTGTGGA CTCTAAGAAG AATTTTTATT TCTTGGAAAT GAATACAAGA 
CTCCAGGTTG AGCATCCTGT CACAGAATGC ATTACTGGCC TGGACCTAGT CCAGGAAATG 
ATCCGTGTTG CTAAGGGCTA CCCTCTCAGG CACAAACAAG CTGATATTCG CATCAACGGC 
TGGGCAGTTG AATGTCGGGT TTATGCTGAG GACCCCTACA AGTCTTTTGG TTTACCATCT 
ATTGGGAGAT TGTCTCAGTA CCAAGAACCG TTACATCTAC CTGGTGTCCG AGTGGACAGT 
GGCATCCAAC CAGGAAGTGA TATTAGCATT TATTATGATC CTATGATTTC AAAACTAATC 
ACATATGGCT CTGATAGAAC TGAGGCACTG AAGAGAATGG CAGATGCACT GGATAACTAT 
GTTATTCGAG GTGTTACACA TAATATTGCA TTACTTCGAG AGGTGATAAT CAACTCACGC 
TTTGTAAAAG GAGACATCAG CACTAAATTT CTCTCCGATG TGTATCCTGA TGGCTTCAAA 
GGACACATGC TAACCAAGAG TGAGAAGAAC CAGTTATTGG CAATAGCATC ATCATTGTTT 
GTGGCATTCC AGTTAAGAGC ACAACATTTT CAAGAAAATT CAAGAATGCC TGTTATTAAA 
CCAGACATAG CCAACTGGGA GCTCTCAGTA AAATTGCATG ATAAAGTTCA TACCGTAGTA 
GCATCAAACA ATGGGTCAGT GTTCTCGGTG GAAGTTGATG GGTCGAAACT AAATGTGACC 
AGCACGTGGA ACCTGGCTTC GCCCTTATTG TCTGTCAGCG TTGATGGCAC TCAGAGGACT 
GTCCAGTGTC TTTCTCGAGA AGCAGGTGGA AACATGAGCA TTCAGTTTCT TGGTACAGTG 
TACAAGGTGA ATATCTTAAC CAGACTTGCC GCAGAATTGA ACAAATTTAT GCTGGAAAAA 
GTGACTGAGG ACACAAGCAG TGTTCTGCGT TCCCCGATGC CCGGAGTGGT GGTGGCCGTC 
TCTGTCAAGC CTGGAGACGC GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876098.1
CDS	114..2201
Translation	MEHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	XM_017020609.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020609.1

ORF Insert Sequence:

ATGGAACATG TTCTGTACTA TTCAAGACAG TGCTTAATGG TGTCCCGTAA TCTTGGTTCA 
GTGGGATATG ATCCTAATGA AAAAACTTTT GATAAAATTC TTGTTGCTAA TAGAGGAGAA 
ATTGCATGTC GGGTTATTAG AACTTGCAAG AAGATGGGCA TTAAGACAGT TGCCATCCAC 
AGTGATGTTG ATGCTAGTTC TGTTCATGTG AAAATGGCGG ATGAGGCTGT CTGTGTTGGC 
CCAGCTCCCA CCAGTAAAAG CTACCTCAAC ATGGATGCCA TCATGGAAGC CATTAAGAAA 
ACCAGGGCCC AAGCTGTACA TCCAGGTTAT GGATTCCTTT CAGAAAACAA AGAATTTGCC 
AGATGTTTGG CAGCAGAAGA TGTCGTTTTC ATTGGACCTG ACACACATGC TATTCAAGCC 
ATGGGCGACA AGATTGAAAG CAAATTATTA GCTAAGAAAG CAGAGGTTAA TACAATCCCT 
GGCTTTGATG GAGTAGTCAA GGATGCAGAA GAAGCTGTCA GAATTGCAAG GGAAATTGGC 
TACCCTGTCA TGATCAAGGC CTCAGCAGGT GGTGGTGGGA AAGGCATGCG CATTGCTTGG 
GATGATGAAG AGACCAGGGA TGGTTTTAGA TTGTCATCTC AAGAAGCTGC TTCTAGTTTT 
GGCGATGATA GACTACTAAT AGAAAAATTT ATTGATAATC CTCGTCATAT AGAAATCCAG 
GTTCTAGGTG ATAAACATGG GAATGCTTTA TGGCTTAATG AAAGAGAGTG CTCAATTCAG 
AGAAGAAATC AGAAGGTGGT GGAGGAAGCA CCAAGCATTT TTTTGGATGC GGAGACTCGA 
AGAGCGATGG GAGAACAAGC TGTAGCTCTT GCCAGAGCAG TAAAATATTC CTCTGCTGGG 
ACCGTGGAGT TCCTTGTGGA CTCTAAGAAG AATTTTTATT TCTTGGAAAT GAATACAAGA 
CTCCAGGTTG AGCATCCTGT CACAGAATGC ATTACTGGCC TGGACCTAGT CCAGGAAATG 
ATCCGTGTTG CTAAGGGCTA CCCTCTCAGG CACAAACAAG CTGATATTCG CATCAACGGC 
TGGGCAGTTG AATGTCGGGT TTATGCTGAG GACCCCTACA AGTCTTTTGG TTTACCATCT 
ATTGGGAGAT TGTCTCAGTA CCAAGAACCG TTACATCTAC CTGGTGTCCG AGTGGACAGT 
GGCATCCAAC CAGGAAGTGA TATTAGCATT TATTATGATC CTATGATTTC AAAACTAATC 
ACATATGGCT CTGATAGAAC TGAGGCACTG AAGAGAATGG CAGATGCACT GGATAACTAT 
GTTATTCGAG GTGTTACACA TAATATTGCA TTACTTCGAG AGGTGATAAT CAACTCACGC 
TTTGTAAAAG GAGACATCAG CACTAAATTT CTCTCCGATG TGTATCCTGA TGGCTTCAAA 
GGACACATGC TAACCAAGAG TGAGAAGAAC CAGTTATTGG CAATAGCATC ATCATTGTTT 
GTGGCATTCC AGTTAAGAGC ACAACATTTT CAAGAAAATT CAAGAATGCC TGTTATTAAA 
CCAGACATAG CCAACTGGGA GCTCTCAGTA AAATTGCATG ATAAAGTTCA TACCGTAGTA 
GCATCAAACA ATGGGTCAGT GTTCTCGGTG GAAGTTGATG GGTCGAAACT AAATGTGACC 
AGCACGTGGA ACCTGGCTTC GCCCTTATTG TCTGTCAGCG TTGATGGCAC TCAGAGGACT 
GTCCAGTGTC TTTCTCGAGA AGCAGGTGGA AACATGAGCA TTCAGTTTCT TGGTACAGTG 
TACAAGGTGA ATATCTTAAC CAGACTTGCC GCAGAATTGA ACAAATTTAT GCTGGAAAAA 
GTGACTGAGG ACACAAGCAG TGTTCTGCGT TCCCCGATGC CCGGAGTGGT GGTGGCCGTC 
TCTGTCAAGC CTGGAGACGC GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020610.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876099.1 Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu84559
Clone ID Related Accession (Same CDS sequence)	XM_017020610.1
Accession Version	XM_017020610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCA GGGACACTCA CTGCATCCCT CCATTCAGTC CCTCTAATTA TCCCAACTTC 
CTGGGGAGCT TGCTTAGAAC TGGGCATGAG TGCTCTTTAT TAAAATATTG GGCTACCCTG 
GCTACATGTA ACAAGGTTCA TGTGAAAATG GCGGATGAGG CTGTCTGTGT TGGCCCAGCT 
CCCACCAGTA AAAGCTACCT CAACATGGAT GCCATCATGG AAGCCATTAA GAAAACCAGG 
GCCCAAGCTG TACATCCAGG TTATGGATTC CTTTCAGAAA ACAAAGAATT TGCCAGATGT 
TTGGCAGCAG AAGATGTCGT TTTCATTGGA CCTGACACAC ATGCTATTCA AGCCATGGGC 
GACAAGATTG AAAGCAAATT ATTAGCTAAG AAAGCAGAGG TTAATACAAT CCCTGGCTTT 
GATGGAGTAG TCAAGGATGC AGAAGAAGCT GTCAGAATTG CAAGGGAAAT TGGCTACCCT 
GTCATGATCA AGGCCTCAGC AGGTGGTGGT GGGAAAGGCA TGCGCATTGC TTGGGATGAT 
GAAGAGACCA GGGATGGTTT TAGATTGTCA TCTCAAGAAG CTGCTTCTAG TTTTGGCGAT 
GATAGACTAC TAATAGAAAA ATTTATTGAT AATCCTCGTC ATATAGAAAT CCAGGTTCTA 
GGTGATAAAC ATGGGAATGC TTTATGGCTT AATGAAAGAG AGTGCTCAAT TCAGAGAAGA 
AATCAGAAGG TGGTGGAGGA AGCACCAAGC ATTTTTTTGG ATGCGGAGAC TCGAAGAGCG 
ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
GAGGTTCTCC AGGTCATGTG CCGGTATAAC AGCCATGCTG TGGGGCCTGT GCTTTCTCAT 
TCCTGCATGG CAAGGCCAGT GTCTTTCTCG AGAAGCAGGT GGAAACATGA GCATTCAGTT 
TCTTGGTACA GTGGAGCACG GGGCTCAGGG AAAGTTCAGC ATTGTCAGTT GTCAACAAAA 
TCAGTAAGTG TTATTAAATA CCCCATGGTG TGTGGTTCTG GCACTGAAAT GGGGCTGTTT 
CCTAAGGCTC TGTCCATGGC CACCTCTGTC TATTTCTGCA TTCTGTCTTG CATTGCTTCT 
GTTGCCTATA TCCTGGTCAG CACAGTTTAT GGTGAATCAT TGCTTATTGA AATAGAGACG 
GCAATAATGA AGCCAGCTAT TATAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876099.1
CDS	534..2603
Translation	MLCRDTHCIPPFSPSNYPNFLGSLLRTGHECSLLKYWATLATCNKVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQEVLQVMCRYNSHAVGPVLSHSCMARPVSFSRSRWKHEHSVSWYSGARGSGKVQHCQLSTKSVSVIKYPMVCGSGTEMGLFPKALSMATSVYFCILSCIASVAYILVSTVYGESLLIEIETAIMKPAIIN

Target ORF information:

RefSeq Version	XM_017020610.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020610.1

ORF Insert Sequence:

ATGCTGTGCA GGGACACTCA CTGCATCCCT CCATTCAGTC CCTCTAATTA TCCCAACTTC 
CTGGGGAGCT TGCTTAGAAC TGGGCATGAG TGCTCTTTAT TAAAATATTG GGCTACCCTG 
GCTACATGTA ACAAGGTTCA TGTGAAAATG GCGGATGAGG CTGTCTGTGT TGGCCCAGCT 
CCCACCAGTA AAAGCTACCT CAACATGGAT GCCATCATGG AAGCCATTAA GAAAACCAGG 
GCCCAAGCTG TACATCCAGG TTATGGATTC CTTTCAGAAA ACAAAGAATT TGCCAGATGT 
TTGGCAGCAG AAGATGTCGT TTTCATTGGA CCTGACACAC ATGCTATTCA AGCCATGGGC 
GACAAGATTG AAAGCAAATT ATTAGCTAAG AAAGCAGAGG TTAATACAAT CCCTGGCTTT 
GATGGAGTAG TCAAGGATGC AGAAGAAGCT GTCAGAATTG CAAGGGAAAT TGGCTACCCT 
GTCATGATCA AGGCCTCAGC AGGTGGTGGT GGGAAAGGCA TGCGCATTGC TTGGGATGAT 
GAAGAGACCA GGGATGGTTT TAGATTGTCA TCTCAAGAAG CTGCTTCTAG TTTTGGCGAT 
GATAGACTAC TAATAGAAAA ATTTATTGAT AATCCTCGTC ATATAGAAAT CCAGGTTCTA 
GGTGATAAAC ATGGGAATGC TTTATGGCTT AATGAAAGAG AGTGCTCAAT TCAGAGAAGA 
AATCAGAAGG TGGTGGAGGA AGCACCAAGC ATTTTTTTGG ATGCGGAGAC TCGAAGAGCG 
ATGGGAGAAC AAGCTGTAGC TCTTGCCAGA GCAGTAAAAT ATTCCTCTGC TGGGACCGTG 
GAGTTCCTTG TGGACTCTAA GAAGAATTTT TATTTCTTGG AAATGAATAC AAGACTCCAG 
GTTGAGCATC CTGTCACAGA ATGCATTACT GGCCTGGACC TAGTCCAGGA AATGATCCGT 
GTTGCTAAGG GCTACCCTCT CAGGCACAAA CAAGCTGATA TTCGCATCAA CGGCTGGGCA 
GTTGAATGTC GGGTTTATGC TGAGGACCCC TACAAGTCTT TTGGTTTACC ATCTATTGGG 
AGATTGTCTC AGTACCAAGA ACCGTTACAT CTACCTGGTG TCCGAGTGGA CAGTGGCATC 
CAACCAGGAA GTGATATTAG CATTTATTAT GATCCTATGA TTTCAAAACT AATCACATAT 
GGCTCTGATA GAACTGAGGC ACTGAAGAGA ATGGCAGATG CACTGGATAA CTATGTTATT 
CGAGGTGTTA CACATAATAT TGCATTACTT CGAGAGGTGA TAATCAACTC ACGCTTTGTA 
AAAGGAGACA TCAGCACTAA ATTTCTCTCC GATGTGTATC CTGATGGCTT CAAAGGACAC 
ATGCTAACCA AGAGTGAGAA GAACCAGTTA TTGGCAATAG CATCATCATT GTTTGTGGCA 
TTCCAGTTAA GAGCACAACA TTTTCAAGAA AATTCAAGAA TGCCTGTTAT TAAACCAGAC 
ATAGCCAACT GGGAGCTCTC AGTAAAATTG CATGATAAAG TTCATACCGT AGTAGCATCA 
AACAATGGGT CAGTGTTCTC GGTGGAAGTT GATGGGTCGA AACTAAATGT GACCAGCACG 
TGGAACCTGG CTTCGCCCTT ATTGTCTGTC AGCGTTGATG GCACTCAGAG GACTGTCCAG 
GAGGTTCTCC AGGTCATGTG CCGGTATAAC AGCCATGCTG TGGGGCCTGT GCTTTCTCAT 
TCCTGCATGG CAAGGCCAGT GTCTTTCTCG AGAAGCAGGT GGAAACATGA GCATTCAGTT 
TCTTGGTACA GTGGAGCACG GGGCTCAGGG AAAGTTCAGC ATTGTCAGTT GTCAACAAAA 
TCAGTAAGTG TTATTAAATA CCCCATGGTG TGTGGTTCTG GCACTGAAAT GGGGCTGTTT 
CCTAAGGCTC TGTCCATGGC CACCTCTGTC TATTTCTGCA TTCTGTCTTG CATTGCTTCT 
GTTGCCTATA TCCTGGTCAG CACAGTTTAT GGTGAATCAT TGCTTATTGA AATAGAGACG 
GCAATAATGA AGCCAGCTAT TATAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020611.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876100.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu84560
Clone ID Related Accession (Same CDS sequence)	XM_017020611.1
Accession Version	XM_017020611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGGAGGT TCTCCAGGTC 
ATGTGCCGGT ATAACAGCCA TGCTGTGGGG CCTGTGCTTT CTCATTCCTG CATGGCAAGG 
CCAGTGTCTT TCTCGAGAAG CAGGTGGAAA CATGAGCATT CAGTTTCTTG GTACAGTGGA 
GCACGGGGCT CAGGGAAAGT TCAGCATTGT CAGTTGTCAA CAAAATCACC CTGGAGAAAG 
CTTCTGGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876100.1
CDS	102..2153
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQEVLQVMCRYNSHAVGPVLSHSCMARPVSFSRSRWKHEHSVSWYSGARGSGKVQHCQLSTKSPWRKLLD

Target ORF information:

RefSeq Version	XM_017020611.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020611.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGGAGGT TCTCCAGGTC 
ATGTGCCGGT ATAACAGCCA TGCTGTGGGG CCTGTGCTTT CTCATTCCTG CATGGCAAGG 
CCAGTGTCTT TCTCGAGAAG CAGGTGGAAA CATGAGCATT CAGTTTCTTG GTACAGTGGA 
GCACGGGGCT CAGGGAAAGT TCAGCATTGT CAGTTGTCAA CAAAATCACC CTGGAGAAAG 
CTTCTGGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020612.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876101.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu84561
Clone ID Related Accession (Same CDS sequence)	XM_017020612.1
Accession Version	XM_017020612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGGAGGT TCTCCAGGTC 
ATGTGCCGGT ATAACAGCCA TGCTGTGGGG CCTGTGCTTT CTCATTCCTG CATGGCAAGG 
CCAGTGTCTT TCTCGAGAAG CAGGTGGAAA CATGAGCATT CAGTTTCTTG GTACAGTGTA 
CAAGGTGAAT ATCTTAACCA GACTTGCCGC AGAATTGAAC AAATTTATGC TGGAAAAAGT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876101.1
CDS	102..2147
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQEVLQVMCRYNSHAVGPVLSHSCMARPVSFSRSRWKHEHSVSWYSVQGEYLNQTCRRIEQIYAGKSD

Target ORF information:

RefSeq Version	XM_017020612.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020612.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGGAGGT TCTCCAGGTC 
ATGTGCCGGT ATAACAGCCA TGCTGTGGGG CCTGTGCTTT CTCATTCCTG CATGGCAAGG 
CCAGTGTCTT TCTCGAGAAG CAGGTGGAAA CATGAGCATT CAGTTTCTTG GTACAGTGTA 
CAAGGTGAAT ATCTTAACCA GACTTGCCGC AGAATTGAAC AAATTTATGC TGGAAAAAGT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020613.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876102.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu84562
Clone ID Related Accession (Same CDS sequence)	XM_017020613.1
Accession Version	XM_017020613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGGAGGT TCTCCAGGTC 
ATGTGCCGGT ATAACAGCCA TGCTGTGGGG CCTGTGCTTT CTCATTCCTG CATGGCAAGG 
CCAGTGTCTT TCTCGAGAAG CAGGTGGAAA CATGAGCATT CAGTTTCTTG GTACAGTGGT 
AGCAGAAGGT CAAGAAATTT GTGTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876102.1
CDS	102..2111
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQEVLQVMCRYNSHAVGPVLSHSCMARPVSFSRSRWKHEHSVSWYSGSRRSRNLCD

Target ORF information:

RefSeq Version	XM_017020613.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020613.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGGTGG AAGTTGATGG GTCGAAACTA AATGTGACCA GCACGTGGAA CCTGGCTTCG 
CCCTTATTGT CTGTCAGCGT TGATGGCACT CAGAGGACTG TCCAGGAGGT TCTCCAGGTC 
ATGTGCCGGT ATAACAGCCA TGCTGTGGGG CCTGTGCTTT CTCATTCCTG CATGGCAAGG 
CCAGTGTCTT TCTCGAGAAG CAGGTGGAAA CATGAGCATT CAGTTTCTTG GTACAGTGGT 
AGCAGAAGGT CAAGAAATTT GTGTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020614.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876103.1 Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu84563
Clone ID Related Accession (Same CDS sequence)	NM_001352607.1 , NM_001352607.2 , XM_017020614.1
Accession Version	XM_017020614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGTT GAGCATCCTG TCACAGAATG CATTACTGGC 
CTGGACCTAG TCCAGGAAAT GATCCGTGTT GCTAAGGGCT ACCCTCTCAG GCACAAACAA 
GCTGATATTC GCATCAACGG CTGGGCAGTT GAATGTCGGG TTTATGCTGA GGACCCCTAC 
AAGTCTTTTG GTTTACCATC TATTGGGAGA TTGTCTCAGT ACCAAGAACC GTTACATCTA 
CCTGGTGTCC GAGTGGACAG TGGCATCCAA CCAGGAAGTG ATATTAGCAT TTATTATGAT 
CCTATGATTT CAAAACTAAT CACATATGGC TCTGATAGAA CTGAGGCACT GAAGAGAATG 
GCAGATGCAC TGGATAACTA TGTTATTCGA GGTGTTACAC ATAATATTGC ATTACTTCGA 
GAGGTGATAA TCAACTCACG CTTTGTAAAA GGAGACATCA GCACTAAATT TCTCTCCGAT 
GTGTATCCTG ATGGCTTCAA AGGACACATG CTAACCAAGA GTGAGAAGAA CCAGTTATTG 
GCAATAGCAT CATCATTGTT TGTGGCATTC CAGTTAAGAG CACAACATTT TCAAGAAAAT 
TCAAGAATGC CTGTTATTAA ACCAGACATA GCCAACTGGG AGCTCTCAGT AAAATTGCAT 
GATAAAGTTC ATACCGTAGT AGCATCAAAC AATGGGTCAG TGTTCTCGGT GGAAGTTGAT 
GGGTCGAAAC TAAATGTGAC CAGCACGTGG AACCTGGCTT CGCCCTTATT GTCTGTCAGC 
GTTGATGGCA CTCAGAGGAC TGTCCAGTGT CTTTCTCGAG AAGCAGGTGG AAACATGAGC 
ATTCAGTTTC TTGGTACAGT GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876103.1
CDS	31..1998
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE

Target ORF information:

RefSeq Version	XM_017020614.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens propionyl-CoA carboxylase alpha subunit (PCCA), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020614.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGACTTT TGATAAAATT 
CTTGTTGCTA ATAGAGGAGA AATTGCATGT CGGGTTATTA GAACTTGCAA GAAGATGGGC 
ATTAAGACAG TTGCCATCCA CAGTGATGTT GATGCTAGTT CTGTTCATGT GAAAATGGCG 
GATGAGGCTG TCTGTGTTGG CCCAGCTCCC ACCAGTAAAA GCTACCTCAA CATGGATGCC 
ATCATGGAAG CCATTAAGAA AACCAGGGCC CAAGCTGTAC ATCCAGGTTA TGGATTCCTT 
TCAGAAAACA AAGAATTTGC CAGATGTTTG GCAGCAGAAG ATGTCGTTTT CATTGGACCT 
GACACACATG CTATTCAAGC CATGGGCGAC AAGATTGAAA GCAAATTATT AGCTAAGAAA 
GCAGAGGTTA ATACAATCCC TGGCTTTGAT GGAGTAGTCA AGGATGCAGA AGAAGCTGTC 
AGAATTGCAA GGGAAATTGG CTACCCTGTC ATGATCAAGG CCTCAGCAGG TGGTGGTGGG 
AAAGGCATGC GCATTGCTTG GGATGATGAA GAGACCAGGG ATGGTTTTAG ATTGTCATCT 
CAAGAAGCTG CTTCTAGTTT TGGCGATGAT AGACTACTAA TAGAAAAATT TATTGATAAT 
CCTCGTCATA TAGAAATCCA GGTTCTAGGT GATAAACATG GGAATGCTTT ATGGCTTAAT 
GAAAGAGAGT GCTCAATTCA GAGAAGAAAT CAGAAGGTGG TGGAGGAAGC ACCAAGCATT 
TTTTTGGATG CGGAGACTCG AAGAGCGATG GGAGAACAAG CTGTAGCTCT TGCCAGAGCA 
GTAAAATATT CCTCTGCTGG GACCGTGGAG TTCCTTGTGG ACTCTAAGAA GAATTTTTAT 
TTCTTGGAAA TGAATACAAG ACTCCAGGTT GAGCATCCTG TCACAGAATG CATTACTGGC 
CTGGACCTAG TCCAGGAAAT GATCCGTGTT GCTAAGGGCT ACCCTCTCAG GCACAAACAA 
GCTGATATTC GCATCAACGG CTGGGCAGTT GAATGTCGGG TTTATGCTGA GGACCCCTAC 
AAGTCTTTTG GTTTACCATC TATTGGGAGA TTGTCTCAGT ACCAAGAACC GTTACATCTA 
CCTGGTGTCC GAGTGGACAG TGGCATCCAA CCAGGAAGTG ATATTAGCAT TTATTATGAT 
CCTATGATTT CAAAACTAAT CACATATGGC TCTGATAGAA CTGAGGCACT GAAGAGAATG 
GCAGATGCAC TGGATAACTA TGTTATTCGA GGTGTTACAC ATAATATTGC ATTACTTCGA 
GAGGTGATAA TCAACTCACG CTTTGTAAAA GGAGACATCA GCACTAAATT TCTCTCCGAT 
GTGTATCCTG ATGGCTTCAA AGGACACATG CTAACCAAGA GTGAGAAGAA CCAGTTATTG 
GCAATAGCAT CATCATTGTT TGTGGCATTC CAGTTAAGAG CACAACATTT TCAAGAAAAT 
TCAAGAATGC CTGTTATTAA ACCAGACATA GCCAACTGGG AGCTCTCAGT AAAATTGCAT 
GATAAAGTTC ATACCGTAGT AGCATCAAAC AATGGGTCAG TGTTCTCGGT GGAAGTTGAT 
GGGTCGAAAC TAAATGTGAC CAGCACGTGG AACCTGGCTT CGCCCTTATT GTCTGTCAGC 
GTTGATGGCA CTCAGAGGAC TGTCCAGTGT CTTTCTCGAG AAGCAGGTGG AAACATGAGC 
ATTCAGTTTC TTGGTACAGT GGTAGCAGAA GGTCAAGAAA TTTGTGTGAT TGAAGCCATG 
AAAATGCAGA ATAGTATGAC AGCTGGGAAA ACTGGCACGG TGAAATCTGT GCACTGTCAA 
GCTGGAGACA CAGTTGGAGA AGGGGATCTG CTCGTGGAGC TGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020615.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876104.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X12, mRNA.

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CloneID	OHu84564
Clone ID Related Accession (Same CDS sequence)	XM_017020615.1
Accession Version	XM_017020615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGAATA TCTTTGGAAG GAGCCACAAG AAACTGGGAC CCATGGTTGC CTGTGAGTTG 
GGGAGCTGGA GGGCTGACTC CATAACAATG GAAGGGTCTG TAAGATGGAG CAGTGTTGAA 
ATAATGGAGC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876104.1
CDS	102..1976
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSNIFGRSHKKLGPMVACELGSWRADSITMEGSVRWSSVEIMEP

Target ORF information:

RefSeq Version	XM_017020615.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020615.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGAATGCCT GTTATTAAAC CAGACATAGC CAACTGGGAG 
CTCTCAGTAA AATTGCATGA TAAAGTTCAT ACCGTAGTAG CATCAAACAA TGGGTCAGTG 
TTCTCGAATA TCTTTGGAAG GAGCCACAAG AAACTGGGAC CCATGGTTGC CTGTGAGTTG 
GGGAGCTGGA GGGCTGACTC CATAACAATG GAAGGGTCTG TAAGATGGAG CAGTGTTGAA 
ATAATGGAGC CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCCA ( XM_017020616.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876105.1 Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu84565
Clone ID Related Accession (Same CDS sequence)	XM_017020616.1
Accession Version	XM_017020616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	propionyl-CoA carboxylase alpha chain, mitochondrial isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGTTGTGCG GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876105.1
CDS	102..1757
Translation	MAGFWVGTAPLVAAGRRGRWPPQQLMLSAALRTLKHVLYYSRQCLMVSRNLGSVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSSCAV

Target ORF information:

RefSeq Version	XM_017020616.1
Organism	Homo sapiens(human)
Definition	Homo sapiens propionyl-CoA carboxylase subunit alpha (PCCA), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020616.1

ORF Insert Sequence:

ATGGCGGGGT TCTGGGTCGG GACAGCACCG CTGGTCGCTG CCGGACGGCG TGGGCGGTGG 
CCGCCGCAGC AGCTGATGCT GAGCGCGGCG CTGCGGACCC TGAAGCATGT TCTGTACTAT 
TCAAGACAGT GCTTAATGGT GTCCCGTAAT CTTGGTTCAG TGGGATATGA TCCTAATGAA 
AAAACTTTTG ATAAAATTCT TGTTGCTAAT AGAGGAGAAA TTGCATGTCG GGTTATTAGA 
ACTTGCAAGA AGATGGGCAT TAAGACAGTT GCCATCCACA GTGATGTTGA TGCTAGTTCT 
GTTCATGTGA AAATGGCGGA TGAGGCTGTC TGTGTTGGCC CAGCTCCCAC CAGTAAAAGC 
TACCTCAACA TGGATGCCAT CATGGAAGCC ATTAAGAAAA CCAGGGCCCA AGCTGTACAT 
CCAGGTTATG GATTCCTTTC AGAAAACAAA GAATTTGCCA GATGTTTGGC AGCAGAAGAT 
GTCGTTTTCA TTGGACCTGA CACACATGCT ATTCAAGCCA TGGGCGACAA GATTGAAAGC 
AAATTATTAG CTAAGAAAGC AGAGGTTAAT ACAATCCCTG GCTTTGATGG AGTAGTCAAG 
GATGCAGAAG AAGCTGTCAG AATTGCAAGG GAAATTGGCT ACCCTGTCAT GATCAAGGCC 
TCAGCAGGTG GTGGTGGGAA AGGCATGCGC ATTGCTTGGG ATGATGAAGA GACCAGGGAT 
GGTTTTAGAT TGTCATCTCA AGAAGCTGCT TCTAGTTTTG GCGATGATAG ACTACTAATA 
GAAAAATTTA TTGATAATCC TCGTCATATA GAAATCCAGG TTCTAGGTGA TAAACATGGG 
AATGCTTTAT GGCTTAATGA AAGAGAGTGC TCAATTCAGA GAAGAAATCA GAAGGTGGTG 
GAGGAAGCAC CAAGCATTTT TTTGGATGCG GAGACTCGAA GAGCGATGGG AGAACAAGCT 
GTAGCTCTTG CCAGAGCAGT AAAATATTCC TCTGCTGGGA CCGTGGAGTT CCTTGTGGAC 
TCTAAGAAGA ATTTTTATTT CTTGGAAATG AATACAAGAC TCCAGGTTGA GCATCCTGTC 
ACAGAATGCA TTACTGGCCT GGACCTAGTC CAGGAAATGA TCCGTGTTGC TAAGGGCTAC 
CCTCTCAGGC ACAAACAAGC TGATATTCGC ATCAACGGCT GGGCAGTTGA ATGTCGGGTT 
TATGCTGAGG ACCCCTACAA GTCTTTTGGT TTACCATCTA TTGGGAGATT GTCTCAGTAC 
CAAGAACCGT TACATCTACC TGGTGTCCGA GTGGACAGTG GCATCCAACC AGGAAGTGAT 
ATTAGCATTT ATTATGATCC TATGATTTCA AAACTAATCA CATATGGCTC TGATAGAACT 
GAGGCACTGA AGAGAATGGC AGATGCACTG GATAACTATG TTATTCGAGG TGTTACACAT 
AATATTGCAT TACTTCGAGA GGTGATAATC AACTCACGCT TTGTAAAAGG AGACATCAGC 
ACTAAATTTC TCTCCGATGT GTATCCTGAT GGCTTCAAAG GACACATGCT AACCAAGAGT 
GAGAAGAACC AGTTATTGGC AATAGCATCA TCATTGTTTG TGGCATTCCA GTTAAGAGCA 
CAACATTTTC AAGAAAATTC AAGTTGTGCG GTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Seventeen Novel Mutations in PCCA and PCCB Genes in Indian Propionic Acidemia Patients, and Their Outcomes.
Genetic testing and molecular biomarkers20(7)373-82(2016 Jul)
Gupta D,Bijarnia-Mahay S,Kohli S,Saxena R,Puri RD,Shigematsu Y,Yamaguchi S,Sakamoto O,Gupta N,Kabra M,Thakur S,Deb R,Verma IC

Frameshift mutations of OGDH, PPAT and PCCA genes in gastric and colorectal cancers.
Neoplasma63(5)681-6(2016)
Jo YS,Oh HR,Kim MS,Yoo NJ,Lee SH

[Analysis of PCCA and PCCB gene mutations in patients with propionic acidemia].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics32(1)26-30(2015 Feb)
Chen Z,Wen P,Wang G,Hu Y,Liu X,Chen L,Chen S,Wan L,Cui D,Shang Y,Li C

Two frequent mutations associated with the classic form of propionic acidemia in Taiwan.
Biochemical genetics52(9-10)415-29(2014 Oct)
Chiu YH,Liu YN,Liao WL,Chang YC,Lin SP,Hsu CC,Chiu PC,Niu DM,Wang CH,Ke YY,Chien YH,Hsiao KJ,Liu TT

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PCCA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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