PC cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PC
Entrez Gene ID	5091
Full Name	pyruvate carboxylase
Synonyms	PCB
General protein information	Preferred Names pyruvate carboxylase Names pyruvate carboxylase, mitochondrial pyruvic carboxylase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.2
Summary	This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	608786
Disorder Html:	Pyruvate carboxylase deficiency, 266150 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006718578.2	XP_006718641.1	pyruvate carboxylase, mitochondrial isoform X1
NM_022172.2	NP_071504.2	pyruvate carboxylase, mitochondrial precursor
XM_017017871.1	XP_016873360.1	pyruvate carboxylase, mitochondrial isoform X1
XM_006718578.3	XP_006718641.1	pyruvate carboxylase, mitochondrial isoform X1
XM_017017872.2	XP_016873361.1	pyruvate carboxylase, mitochondrial isoform X1
XM_005274032.3	XP_005274089.1	pyruvate carboxylase, mitochondrial isoform X1
XM_011545086.1	XP_011543388.1	pyruvate carboxylase, mitochondrial isoform X1
XM_017017872.1	XP_016873361.1	pyruvate carboxylase, mitochondrial isoform X1
NM_022172.3	NP_071504.2	pyruvate carboxylase, mitochondrial precursor
XM_017017868.1	XP_016873357.1	pyruvate carboxylase, mitochondrial isoform X1
XM_005274031.4	XP_005274088.1	pyruvate carboxylase, mitochondrial isoform X1
XM_011545087.2	XP_011543389.1	pyruvate carboxylase, mitochondrial isoform X2
XM_006718579.3	XP_006718642.1	pyruvate carboxylase, mitochondrial isoform X3
NM_000920.4	NP_000911.2	pyruvate carboxylase, mitochondrial precursor
XM_006718577.2	XP_006718640.1	pyruvate carboxylase, mitochondrial isoform X1
NM_001040716.1	NP_001035806.1	pyruvate carboxylase, mitochondrial precursor
NM_001040716.2	NP_001035806.1	pyruvate carboxylase, mitochondrial precursor
XM_017017869.1	XP_016873358.1	pyruvate carboxylase, mitochondrial isoform X1
XM_011545086.2	XP_011543388.1	pyruvate carboxylase, mitochondrial isoform X1
NM_000920.3	NP_000911.2	pyruvate carboxylase, mitochondrial precursor
XM_005274031.3	XP_005274088.1	pyruvate carboxylase, mitochondrial isoform X1
XM_005274032.4	XP_005274089.1	pyruvate carboxylase, mitochondrial isoform X1
XM_011545085.1	XP_011543387.1	pyruvate carboxylase, mitochondrial isoform X1
XM_017017870.1	XP_016873359.1	pyruvate carboxylase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PC	NP_071504.2
Canis lupus familiaris (dog)	PC	XP_540825.2
Bos taurus (cattle)	PC	NP_808815.2
Mus musculus (house mouse)	Pcx	NP_001156418.1
Caenorhabditis elegans (roundworm)	pyc-1	NP_001256376.1
Saccharomyces cerevisiae (baker's yeast)	PYC2	NP_009777.1
Kluyveromyces lactis	KLLA0C05764g	XP_452456.1
Eremothecium gossypii	AGOS_AAR162C	NP_982705.2
Magnaporthe oryzae (rice blast fungus)	MGG_07756	XP_003712969.1
Pan troglodytes (chimpanzee)	PC	XP_003952003.1
Neurospora crassa	NCU02505	XP_965636.1
Xenopus tropicalis (tropical clawed frog)	pc.2	NP_001096495.1
Schizosaccharomyces pombe (fission yeast)	pyr1	NP_595900.1
	PC	XP_001107814.1
Rattus norvegicus (Norway rat)	Pc	NP_036876.2
Danio rerio (zebrafish)	pcxb	NP_571625.1
Drosophila melanogaster (fruit fly)	CG1516	NP_724844.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004742	XP_001689096.1
Saccharomyces cerevisiae (baker's yeast)	PYC1	NP_011453.1

Gene Ontology
Bibliography

Related articles in PubMed

Mass spectrometry analysis shows the biosynthetic pathways supported by pyruvate carboxylase in highly invasive breast cancer cells.
Phannasil P, Ansari IH, El Azzouny M, Longacre MJ, Rattanapornsompong K, Burant CF, MacDonald MJ, Jitrapakdee S
Biochimica et biophysica acta1863(2)537-551(2017 Feb)

Breast Cancer-Derived Lung Metastases Show Increased Pyruvate Carboxylase-Dependent Anaplerosis.
Christen S, Lorendeau D, Schmieder R, Broekaert D, Metzger K, Veys K, Elia I, Buescher JM, Orth MF, Davidson SM, Gr?newald TG, De Bock K, Fendt SM
Cell reports17(3)837-848(2016 10)

Pyruvate Carboxylase Activates the RIG-I-like Receptor-Mediated Antiviral Immune Response by Targeting the MAVS signalosome.
Cao Z, Zhou Y, Zhu S, Feng J, Chen X, Liu S, Peng N, Yang X, Xu G, Zhu Y
Scientific reports622002(2016 Feb)

Pyruvate carboxylase is critical for non-small-cell lung cancer proliferation.
Sellers K, Fox MP, Bousamra M 2nd, Slone SP, Higashi RM, Miller DM, Wang Y, Yan J, Yuneva MO, Deshpande R, Lane AN, Fan TW
The Journal of clinical investigation125(2)687-98(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

breast cancer cells proliferating as lung metastases activate PC-dependent anaplerosis in response to the lung microenvironment.
Title: Breast Cancer-Derived Lung Metastases Show Increased Pyruvate Carboxylase-Dependent Anaplerosis.

Pyruvate carboxylase activates the RIG-I-like receptor-mediated antiviral immune response by targeting the MAVS signalosome.
Title: Pyruvate Carboxylase Activates the RIG-I-like Receptor-Mediated Antiviral Immune Response by Targeting the MAVS signalosome.

These findings indicate that PC-mediated anaplerosis in early-stage NSCLC is required for tumor survival and proliferation.
Title: Pyruvate carboxylase is critical for non-small-cell lung cancer proliferation.

PC and PDH may be an important part of cellular adaptation to the IDH1 mutation and may serve as potential therapeutic targets
Title: Glioma cells with the IDH1 mutation modulate metabolic fractional flux through pyruvate carboxylase.

characterization of PC distal promoter
Title: Characterization of the distal promoter of the human pyruvate carboxylase gene in pancreatic beta cells.

The following PC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16250	NM_022172.3 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	NM_001040716.2 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	NM_000920.4 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_011545086.2 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_017017870.1 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_017017872.2 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_005274031.4 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_011545085.1 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_017017871.1 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_017017869.1 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_006718578.3 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_017017868.1 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_005274032.4 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_006718577.2 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu45353	XM_006718579.3 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu59513	XM_011545087.2 Latest version!	Homo sapiens pyruvate carboxylase (PC), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu16250	XM_006718578.2	Homo sapiens pyruvate carboxylase (PC), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_017017872.1	Homo sapiens pyruvate carboxylase (PC), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	NM_022172.2	Homo sapiens pyruvate carboxylase (PC), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	NM_001040716.1	Homo sapiens pyruvate carboxylase (PC), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_011545086.1	Homo sapiens pyruvate carboxylase (PC), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	NM_000920.3	Homo sapiens pyruvate carboxylase (PC), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_005274031.3	Homo sapiens pyruvate carboxylase (PC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu16250	XM_005274032.3	Homo sapiens pyruvate carboxylase (PC), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PC ( NM_022172.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_071504.2 Homo sapiens pyruvate carboxylase (PC), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	NM_022172.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U30891.1 and S72370.1. On Jun 1, 2006 this sequence version replaced NM_022172.1. Transcript Variant: This variant (2) contains an alternate 5' non-coding exon resulting from the use of a more proximal promoter, hence has a distinct 5' UTR compared to transcript variant 1. Transcript variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U30891.1, S72370.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071504.2
CDS	50..3586
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	NM_022172.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022172.2

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( NM_022172.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_071504.2 Homo sapiens pyruvate carboxylase (PC), transcript variant 2, mRNA.

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CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	NM_022172.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP003176.2, U30891.1, S72370.1 and AP000485.5. On May 31, 2019 this sequence version replaced NM_022172.2. Transcript Variant: This variant (2) contains an alternate 5' non-coding exon resulting from the use of a more proximal promoter, hence has a distinct 5' UTR compared to transcript variant 1. Transcript variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.71299.1, SRR1803617.82711.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071504.2
CDS	134..3670
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	NM_022172.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022172.3

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( NM_000920.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000911.2 Homo sapiens pyruvate carboxylase (PC), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	NM_000920.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP003176.2, DA542083.1, BC011617.2 and AP000485.5. On May 31, 2019 this sequence version replaced NM_000920.3. Transcript Variant: This variant (1) results from the use of a more distal promoter. Transcript variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.2243.1, SRR1803617.230512.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000911.2
CDS	102..3638
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	NM_000920.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000920.4

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( NM_001040716.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035806.1 Homo sapiens pyruvate carboxylase (PC), transcript variant 3, mRNA.

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CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	NM_001040716.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA542083.1, CN267865.1 and BC011617.2. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) also results from the use of a more distal promoter, however, it contains an additional 5' non-coding exon, hence has a longer 5' UTR compared to transcript variant 1. Transcript variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.151860.1, SRR1803611.132999.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035806.1
CDS	283..3819
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	NM_001040716.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040716.1

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( NM_001040716.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035806.1 Homo sapiens pyruvate carboxylase (PC), transcript variant 3, mRNA.

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CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	NM_001040716.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP003176.2, DA542083.1, CN267865.1, BC011617.2 and AP000485.5. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_001040716.1. Transcript Variant: This variant (3) also results from the use of a more distal promoter, however, it contains an additional 5' non-coding exon, hence has a longer 5' UTR compared to transcript variant 1. Transcript variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.145698.1, SRR1803617.27410.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000393960.7/ ENSP00000377532.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035806.1
CDS	290..3826
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	NM_001040716.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040716.2

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( NM_000920.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000911.2 Homo sapiens pyruvate carboxylase (PC), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	NM_000920.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA542083.1 and BC011617.2. On Jun 1, 2006 this sequence version replaced NM_000920.2. Transcript Variant: This variant (1) results from the use of a more distal promoter. Transcript variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.221889.1, SRR1660807.83901.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000911.2
CDS	95..3631
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	NM_000920.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000920.3

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_006718578.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718641.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X5, mRNA.

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CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_006718578.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006718641.1

Translation

MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_006718578.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718578.2

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_017017871.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873360.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_017017871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873360.1
CDS	265..3801
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_017017871.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017871.1

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_006718578.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718641.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_006718578.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718578.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718641.1
CDS	95..3631
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_006718578.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718578.3

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_017017872.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873361.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X6, mRNA.

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CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_017017872.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017017872.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873361.1
CDS	648..4184
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_017017872.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017872.2

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005274032.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274089.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X4, mRNA.

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CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_005274032.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005274089.1

Translation

MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005274032.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274032.3

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_011545086.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543388.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X6, mRNA.

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CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_011545086.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011543388.1

Translation

MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_011545086.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545086.1

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_017017872.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873361.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_017017872.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873361.1
CDS	647..4183
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_017017872.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017872.1

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_017017868.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873357.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_017017868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873357.1
CDS	582..4118
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_017017868.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017868.1

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005274031.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274088.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X7, mRNA.

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CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_005274031.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005274031.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274088.1
CDS	142..3678
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005274031.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274031.4

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_006718577.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718640.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X1, mRNA.

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CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_006718577.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006718640.1

Translation

MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_006718577.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718577.2

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_017017869.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873358.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_017017869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873358.1
CDS	453..3989
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_017017869.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017869.1

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_011545086.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543388.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X9, mRNA.

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CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_011545086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543388.1
CDS	411..3947
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_011545086.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545086.2

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005274031.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274088.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X2, mRNA.

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CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_005274031.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005274088.1

Translation

MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005274031.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274031.3

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005274032.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274089.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_005274032.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005274032.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274089.1
CDS	24..3560
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005274032.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274032.4

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_011545085.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543387.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_011545085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011543387.1

Translation

MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_011545085.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545085.1

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_017017870.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873359.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu16250
Clone ID Related Accession (Same CDS sequence)	XM_006718578.2 , NM_022172.2 , XM_017017871.1 , XM_006718578.3 , XM_017017872.2 , XM_005274032.3 , XM_011545086.1 , XM_017017872.1 , NM_022172.3 , XM_017017868.1 , XM_005274031.4 , NM_000920.4 , XM_006718577.2 , NM_001040716.1 , NM_001040716.2 , XM_017017869.1 , XM_011545086.2 , NM_000920.3 , XM_005274031.3 , XM_005274032.4 , XM_011545085.1 , XM_017017870.1
Accession Version	XM_017017870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873359.1
CDS	394..3930
Translation	MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_017017870.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017870.1

ORF Insert Sequence:

ATGCTGAAGT TCCGAACAGT CCATGGGGGC CTGAGGCTCC TGGGAATCCG CCGAACCTCC 
ACCGCCCCCG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ATAAGCCCAT CAAGAAAGTC 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGTGTGTTCC GGGCCTGCAC GGAGCTGGGC 
ATCCGCACCG TAGCCATCTA CTCTGAGCAG GACACGGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ATCTCATCGG CCGCGGCCTG GCCCCCGTGC AGGCCTACCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAGAACAAC GTAGATGCAG TGCACCCTGG CTACGGGTTC 
CTCTCTGAGC GAGCGGACTT CGCCCAGGCC TGCCAGGATG CAGGGGTCCG GTTTATTGGG 
CCAAGCCCAG AAGTGGTCCG CAAGATGGGA GACAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCGGGTG TTCCCGTTGT CCCTGGCACA GATGCCCCCA TCACGTCCCT GCATGAGGCC 
CACGAGTTCT CCAACACCTA CGGCTTCCCC ATCATCTTCA AGGCGGCCTA TGGGGGTGGA 
GGGCGTGGCA TGAGGGTGGT GCACAGCTAC GAGGAGCTGG AGGAGAATTA CACCCGGGCC 
TACTCAGAGG CTCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATCGAG 
AAGCCACGGC ACATCGAGGT GCAGATCTTG GGGGACCAGT ATGGGAACAT CCTGCACCTG 
TACGAGCGAG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTCGAGAT TGCCCCCGCC 
GCCCACCTGG ACCCGCAGCT TCGGACTCGG CTCACCAGCG ACTCTGTGAA ACTCGCTAAA 
CAGGTGGGCT ACGAGAACGC AGGCACCGTG GAGTTCCTGG TGGACAGGCA CGGCAAGCAC 
TACTTCATCG AGGTCAACTC CCGCCTGCAG GTGGAGCACA CGGTCACAGA GGAGATCACC 
GACGTAGACC TGGTCCATGC TCAGATCCAC GTGGCTGAGG GCAGGAGCCT ACCCGACCTG 
GGCCTGCGGC AGGAGAACAT CCGCATCAAC GGGTGTGCCA TCCAGTGCCG GGTCACCACC 
GAGGACCCCG CGCGCAGCTT CCAGCCGGAC ACCGGCCGCA TTGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCT TCCGCCTTCC AAGGAGCCGT CATCTCGCCC 
CACTACGACT CCCTGCTGGT CAAAGTCATT GCCCACGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGGGCCCT TGCGGAGTTC CGCGTCCGAG GTGTGAAGAC CAACATCGCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACTGTGGA CACCCAGTTC 
ATCGACGAGA ACCCAGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAAAAGCTG 
TTGCACTACC TCGGCCATGT CATGGTAAAC GGTCCAACCA CCCCGATTCC CGTCAAGGCC 
AGCCCCAGCC CCACGGACCC CGTTGTCCCT GCAGTGCCCA TAGGCCCGCC CCCGGCTGGT 
TTCAGAGACA TCCTGCTGCG AGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GCGGAACCAC 
CCGGGGCTGC TGCTGATGGA CACGACCTTC AGGGACGCCC ACCAGTCACT GCTGGCCACT 
CGTGTGCGCA CCCACGATCT CAAAAAGATC GCCCCCTATG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TGGAGAACTG GGGAGGAGCC ACGTTTGACG TCGCCATGCG CTTCCTGTAT 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCTTTCCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCAGACAA CGTGGTCTTC 
AAGTTCTGTG AAGTGGCCAA AGAGAATGGC ATGGATGTCT TCCGTGTGTT TGACTCCCTC 
AACTACTTGC CCAACATGCT GCTGGGCATG GAGGCGGCAG GAAGTGCCGG AGGCGTGGTG 
GAGGCTGCCA TCTCATACAC GGGCGACGTG GCCGACCCCA GCCGCACCAA GTACTCACTG 
CAGTACTACA TGGGCTTGGC CGAAGAGCTG GTGCGAGCTG GCACCCACAT CCTGTGCATC 
AAGGACATGG CCGGGCTGCT GAAGCCCACG GCCTGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGCTTCC CCGACCTCCC ACTGCACATC CACACCCACG ACACGTCAGG GGCAGGCGTG 
GCAGCCATGC TGGCCTGTGC CCAGGCTGGA GCTGATGTGG TGGATGTGGC AGCTGATTCC 
ATGTCTGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAGAGGGACT 
CCCCTGGACA CAGAGGTGCC CATGGAGCGC GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCTCGGGGAC TGTACGCGGC CTTCGACTGC ACGGCCACCA TGAAGTCTGG CAACTCGGAC 
GTGTATGAAA ATGAGATCCC AGGGGGCCAG TACACCAACC TGCACTTCCA GGCCCACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACGCCCTCC TCCAAGATCG TGGGGGACCT GGCCCAGTTT 
ATGGTGCAGA ATGGATTGAG CCGGGCAGAG GCCGAAGCTC AGGCGGAAGA GCTGTCCTTT 
CCCCGCTCCG TGGTGGAGTT CCTGCAGGGC TACATCGGTG TCCCCCATGG GGGGTTCCCC 
GAACCCTTTC GCTCTAAGGT ACTGAAGGAC CTGCCAAGGG TGGAGGGGCG GCCTGGAGCC 
TCCCTCCCTC CCCTGGATCT GCAGGCACTG GAGAAGGAGC TGGTAGACCG GCATGGGGAG 
GAGGTGACGC CGGAAGATGT GCTCTCAGCA GCTATGTACC CCGATGTGTT TGCCCACTTC 
AAGGACTTCA CTGCCACCTT TGGCCCCCTG GATAGCCTGA ATACTCGCCT CTTCCTGCAG 
GGACCCAAGA TCGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC GCTGCACATC 
AAAGCCCTGG CCGTGAGCGA CCTGAACCGG GCCGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGGCAGC TGCGGTCCAT CTTGGTCAAG GACACCCAGG CCATGAAGGA GATGCACTTC 
CACCCCAAGG CCCTAAAGGA CGTGAAGGGC CAGATCGGGG CGCCCATGCC TGGGAAGGTG 
ATAGACATCA AAGTGGTGGC AGGGGCCAAG GTGGCCAAGG GCCAGCCCCT GTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACC TCACCCATGG AGGGTACTGT CCGCAAGGTT 
CATGTGACCA AGGACATGAC ACTGGAAGGT GACGACCTCA TCCTGGAGAT CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_006718579.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718642.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X11, mRNA.

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CloneID	OHu45353
Clone ID Related Accession (Same CDS sequence)	XM_006718579.3
Accession Version	XM_006718579.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718579.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAAACG GTCCAACCAC CCCGATTCCC GTCAAGGCCA GCCCCAGCCC CACGGACCCC 
GTTGTCCCTG CAGTGCCCAT AGGCCCGCCC CCGGCTGGTT TCAGAGACAT CCTGCTGCGA 
GAGGGGCCTG AGGGCTTTGC TCGAGCTGTG CGGAACCACC CGGGGCTGCT GCTGATGGAC 
ACGACCTTCA GGGACGCCCA CCAGTCACTG CTGGCCACTC GTGTGCGCAC CCACGATCTC 
AAAAAGATCG CCCCCTATGT TGCCCACAAC TTCAGCAAGC TCTTCAGCAT GGAGAACTGG 
GGAGGAGCCA CGTTTGACGT CGCCATGCGC TTCCTGTATG AGTGCCCCTG GCGGCGGCTG 
CAGGAGCTCC GGGAGCTCAT CCCCAACATC CCTTTCCAGA TGCTGCTGCG GGGGGCCAAT 
GCTGTGGGCT ACACCAACTA CCCAGACAAC GTGGTCTTCA AGTTCTGTGA AGTGGCCAAA 
GAGAATGGCA TGGATGTCTT CCGTGTGTTT GACTCCCTCA ACTACTTGCC CAACATGCTG 
CTGGGCATGG AGGCGGCAGG AAGTGCCGGA GGCGTGGTGG AGGCTGCCAT CTCATACACG 
GGCGACGTGG CCGACCCCAG CCGCACCAAG TACTCACTGC AGTACTACAT GGGCTTGGCC 
GAAGAGCTGG TGCGAGCTGG CACCCACATC CTGTGCATCA AGGACATGGC CGGGCTGCTG 
AAGCCCACGG CCTGCACCAT GCTGGTCAGC TCCCTCCGGG ACCGCTTCCC CGACCTCCCA 
CTGCACATCC ACACCCACGA CACGTCAGGG GCAGGCGTGG CAGCCATGCT GGCCTGTGCC 
CAGGCTGGAG CTGATGTGGT GGATGTGGCA GCTGATTCCA TGTCTGGGAT GACTTCACAG 
CCCAGCATGG GGGCCCTGGT GGCCTGTACC AGAGGGACTC CCCTGGACAC AGAGGTGCCC 
ATGGAGCGCG TGTTTGACTA CAGTGAGTAC TGGGAGGGGG CTCGGGGACT GTACGCGGCC 
TTCGACTGCA CGGCCACCAT GAAGTCTGGC AACTCGGACG TGTATGAAAA TGAGATCCCA 
GGGGGCCAGT ACACCAACCT GCACTTCCAG GCCCACAGCA TGGGGCTTGG CTCCAAGTTC 
AAGGAGGTCA AGAAGGCCTA TGTGGAGGCC AACCAGATGC TGGGCGATCT CATCAAGGTG 
ACGCCCTCCT CCAAGATCGT GGGGGACCTG GCCCAGTTTA TGGTGCAGAA TGGATTGAGC 
CGGGCAGAGG CCGAAGCTCA GGCGGAAGAG CTGTCCTTTC CCCGCTCCGT GGTGGAGTTC 
CTGCAGGGCT ACATCGGTGT CCCCCATGGG GGGTTCCCCG AACCCTTTCG CTCTAAGGTA 
CTGAAGGACC TGCCAAGGGT GGAGGGGCGG CCTGGAGCCT CCCTCCCTCC CCTGGATCTG 
CAGGCACTGG AGAAGGAGCT GGTAGACCGG CATGGGGAGG AGGTGACGCC GGAAGATGTG 
CTCTCAGCAG CTATGTACCC CGATGTGTTT GCCCACTTCA AGGACTTCAC TGCCACCTTT 
GGCCCCCTGG ATAGCCTGAA TACTCGCCTC TTCCTGCAGG GACCCAAGAT CGCAGAGGAG 
TTTGAGGTGG AGCTGGAGCG GGGCAAGACG CTGCACATCA AAGCCCTGGC CGTGAGCGAC 
CTGAACCGGG CCGGCCAGAG GCAGGTCTTC TTTGAGCTCA ATGGGCAGCT GCGGTCCATC 
TTGGTCAAGG ACACCCAGGC CATGAAGGAG ATGCACTTCC ACCCCAAGGC CCTAAAGGAC 
GTGAAGGGCC AGATCGGGGC GCCCATGCCT GGGAAGGTGA TAGACATCAA AGTGGTGGCA 
GGGGCCAAGG TGGCCAAGGG CCAGCCCCTG TGTGTGCTCA GTGCCATGAA GATGGAGACT 
GTGGTGACCT CACCCATGGA GGGTACTGTC CGCAAGGTTC ATGTGACCAA GGACATGACA 
CTGGAAGGTG ACGACCTCAT CCTGGAGATC GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718642.1
CDS	405..2420
Translation	MVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_006718579.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718579.3

ORF Insert Sequence:

ATGGTAAACG GTCCAACCAC CCCGATTCCC GTCAAGGCCA GCCCCAGCCC CACGGACCCC 
GTTGTCCCTG CAGTGCCCAT AGGCCCGCCC CCGGCTGGTT TCAGAGACAT CCTGCTGCGA 
GAGGGGCCTG AGGGCTTTGC TCGAGCTGTG CGGAACCACC CGGGGCTGCT GCTGATGGAC 
ACGACCTTCA GGGACGCCCA CCAGTCACTG CTGGCCACTC GTGTGCGCAC CCACGATCTC 
AAAAAGATCG CCCCCTATGT TGCCCACAAC TTCAGCAAGC TCTTCAGCAT GGAGAACTGG 
GGAGGAGCCA CGTTTGACGT CGCCATGCGC TTCCTGTATG AGTGCCCCTG GCGGCGGCTG 
CAGGAGCTCC GGGAGCTCAT CCCCAACATC CCTTTCCAGA TGCTGCTGCG GGGGGCCAAT 
GCTGTGGGCT ACACCAACTA CCCAGACAAC GTGGTCTTCA AGTTCTGTGA AGTGGCCAAA 
GAGAATGGCA TGGATGTCTT CCGTGTGTTT GACTCCCTCA ACTACTTGCC CAACATGCTG 
CTGGGCATGG AGGCGGCAGG AAGTGCCGGA GGCGTGGTGG AGGCTGCCAT CTCATACACG 
GGCGACGTGG CCGACCCCAG CCGCACCAAG TACTCACTGC AGTACTACAT GGGCTTGGCC 
GAAGAGCTGG TGCGAGCTGG CACCCACATC CTGTGCATCA AGGACATGGC CGGGCTGCTG 
AAGCCCACGG CCTGCACCAT GCTGGTCAGC TCCCTCCGGG ACCGCTTCCC CGACCTCCCA 
CTGCACATCC ACACCCACGA CACGTCAGGG GCAGGCGTGG CAGCCATGCT GGCCTGTGCC 
CAGGCTGGAG CTGATGTGGT GGATGTGGCA GCTGATTCCA TGTCTGGGAT GACTTCACAG 
CCCAGCATGG GGGCCCTGGT GGCCTGTACC AGAGGGACTC CCCTGGACAC AGAGGTGCCC 
ATGGAGCGCG TGTTTGACTA CAGTGAGTAC TGGGAGGGGG CTCGGGGACT GTACGCGGCC 
TTCGACTGCA CGGCCACCAT GAAGTCTGGC AACTCGGACG TGTATGAAAA TGAGATCCCA 
GGGGGCCAGT ACACCAACCT GCACTTCCAG GCCCACAGCA TGGGGCTTGG CTCCAAGTTC 
AAGGAGGTCA AGAAGGCCTA TGTGGAGGCC AACCAGATGC TGGGCGATCT CATCAAGGTG 
ACGCCCTCCT CCAAGATCGT GGGGGACCTG GCCCAGTTTA TGGTGCAGAA TGGATTGAGC 
CGGGCAGAGG CCGAAGCTCA GGCGGAAGAG CTGTCCTTTC CCCGCTCCGT GGTGGAGTTC 
CTGCAGGGCT ACATCGGTGT CCCCCATGGG GGGTTCCCCG AACCCTTTCG CTCTAAGGTA 
CTGAAGGACC TGCCAAGGGT GGAGGGGCGG CCTGGAGCCT CCCTCCCTCC CCTGGATCTG 
CAGGCACTGG AGAAGGAGCT GGTAGACCGG CATGGGGAGG AGGTGACGCC GGAAGATGTG 
CTCTCAGCAG CTATGTACCC CGATGTGTTT GCCCACTTCA AGGACTTCAC TGCCACCTTT 
GGCCCCCTGG ATAGCCTGAA TACTCGCCTC TTCCTGCAGG GACCCAAGAT CGCAGAGGAG 
TTTGAGGTGG AGCTGGAGCG GGGCAAGACG CTGCACATCA AAGCCCTGGC CGTGAGCGAC 
CTGAACCGGG CCGGCCAGAG GCAGGTCTTC TTTGAGCTCA ATGGGCAGCT GCGGTCCATC 
TTGGTCAAGG ACACCCAGGC CATGAAGGAG ATGCACTTCC ACCCCAAGGC CCTAAAGGAC 
GTGAAGGGCC AGATCGGGGC GCCCATGCCT GGGAAGGTGA TAGACATCAA AGTGGTGGCA 
GGGGCCAAGG TGGCCAAGGG CCAGCCCCTG TGTGTGCTCA GTGCCATGAA GATGGAGACT 
GTGGTGACCT CACCCATGGA GGGTACTGTC CGCAAGGTTC ATGTGACCAA GGACATGACA 
CTGGAAGGTG ACGACCTCAT CCTGGAGATC GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_011545087.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543389.1 Homo sapiens pyruvate carboxylase (PC), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu59513
Clone ID Related Accession (Same CDS sequence)	XM_011545087.2
Accession Version	XM_011545087.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCCA GCAACAGTGT GGGTCTGCGC TCAGCCCTGC CCGTGCAGGT TCCTCAGCGG 
CCTGCTGGAA AGACCAACAT CGCCTTCCTG CAGAATGTGC TCAACAACCA GCAGTTCCTG 
GCAGGCACTG TGGACACCCA GTTCATCGAC GAGAACCCAG AGCTGTTCCA GCTGCGGCCT 
GCACAGAACC GGGCCCAAAA GCTGTTGCAC TACCTCGGCC ATGTCATGGT AAACGGTCCA 
ACCACCCCGA TTCCCGTCAA GGCCAGCCCC AGCCCCACGG ACCCCGTTGT CCCTGCAGTG 
CCCATAGGCC CGCCCCCGGC TGGTTTCAGA GACATCCTGC TGCGAGAGGG GCCTGAGGGC 
TTTGCTCGAG CTGTGCGGAA CCACCCGGGG CTGCTGCTGA TGGACACGAC CTTCAGGGAC 
GCCCACCAGT CACTGCTGGC CACTCGTGTG CGCACCCACG ATCTCAAAAA GATCGCCCCC 
TATGTTGCCC ACAACTTCAG CAAGCTCTTC AGCATGGAGA ACTGGGGAGG AGCCACGTTT 
GACGTCGCCA TGCGCTTCCT GTATGAGTGC CCCTGGCGGC GGCTGCAGGA GCTCCGGGAG 
CTCATCCCCA ACATCCCTTT CCAGATGCTG CTGCGGGGGG CCAATGCTGT GGGCTACACC 
AACTACCCAG ACAACGTGGT CTTCAAGTTC TGTGAAGTGG CCAAAGAGAA TGGCATGGAT 
GTCTTCCGTG TGTTTGACTC CCTCAACTAC TTGCCCAACA TGCTGCTGGG CATGGAGGCG 
GCAGGAAGTG CCGGAGGCGT GGTGGAGGCT GCCATCTCAT ACACGGGCGA CGTGGCCGAC 
CCCAGCCGCA CCAAGTACTC ACTGCAGTAC TACATGGGCT TGGCCGAAGA GCTGGTGCGA 
GCTGGCACCC ACATCCTGTG CATCAAGGAC ATGGCCGGGC TGCTGAAGCC CACGGCCTGC 
ACCATGCTGG TCAGCTCCCT CCGGGACCGC TTCCCCGACC TCCCACTGCA CATCCACACC 
CACGACACGT CAGGGGCAGG CGTGGCAGCC ATGCTGGCCT GTGCCCAGGC TGGAGCTGAT 
GTGGTGGATG TGGCAGCTGA TTCCATGTCT GGGATGACTT CACAGCCCAG CATGGGGGCC 
CTGGTGGCCT GTACCAGAGG GACTCCCCTG GACACAGAGG TGCCCATGGA GCGCGTGTTT 
GACTACAGTG AGTACTGGGA GGGGGCTCGG GGACTGTACG CGGCCTTCGA CTGCACGGCC 
ACCATGAAGT CTGGCAACTC GGACGTGTAT GAAAATGAGA TCCCAGGGGG CCAGTACACC 
AACCTGCACT TCCAGGCCCA CAGCATGGGG CTTGGCTCCA AGTTCAAGGA GGTCAAGAAG 
GCCTATGTGG AGGCCAACCA GATGCTGGGC GATCTCATCA AGGTGACGCC CTCCTCCAAG 
ATCGTGGGGG ACCTGGCCCA GTTTATGGTG CAGAATGGAT TGAGCCGGGC AGAGGCCGAA 
GCTCAGGCGG AAGAGCTGTC CTTTCCCCGC TCCGTGGTGG AGTTCCTGCA GGGCTACATC 
GGTGTCCCCC ATGGGGGGTT CCCCGAACCC TTTCGCTCTA AGGTACTGAA GGACCTGCCA 
AGGGTGGAGG GGCGGCCTGG AGCCTCCCTC CCTCCCCTGG ATCTGCAGGC ACTGGAGAAG 
GAGCTGGTAG ACCGGCATGG GGAGGAGGTG ACGCCGGAAG ATGTGCTCTC AGCAGCTATG 
TACCCCGATG TGTTTGCCCA CTTCAAGGAC TTCACTGCCA CCTTTGGCCC CCTGGATAGC 
CTGAATACTC GCCTCTTCCT GCAGGGACCC AAGATCGCAG AGGAGTTTGA GGTGGAGCTG 
GAGCGGGGCA AGACGCTGCA CATCAAAGCC CTGGCCGTGA GCGACCTGAA CCGGGCCGGC 
CAGAGGCAGG TCTTCTTTGA GCTCAATGGG CAGCTGCGGT CCATCTTGGT CAAGGACACC 
CAGGCCATGA AGGAGATGCA CTTCCACCCC AAGGCCCTAA AGGACGTGAA GGGCCAGATC 
GGGGCGCCCA TGCCTGGGAA GGTGATAGAC ATCAAAGTGG TGGCAGGGGC CAAGGTGGCC 
AAGGGCCAGC CCCTGTGTGT GCTCAGTGCC ATGAAGATGG AGACTGTGGT GACCTCACCC 
ATGGAGGGTA CTGTCCGCAA GGTTCATGTG ACCAAGGACA TGACACTGGA AGGTGACGAC 
CTCATCCTGG AGATCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543389.1
CDS	305..2545
Translation	MGPSNSVGLRSALPVQVPQRPAGKTNIAFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_011545087.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pyruvate carboxylase (PC), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545087.2

ORF Insert Sequence:

ATGGGTCCCA GCAACAGTGT GGGTCTGCGC TCAGCCCTGC CCGTGCAGGT TCCTCAGCGG 
CCTGCTGGAA AGACCAACAT CGCCTTCCTG CAGAATGTGC TCAACAACCA GCAGTTCCTG 
GCAGGCACTG TGGACACCCA GTTCATCGAC GAGAACCCAG AGCTGTTCCA GCTGCGGCCT 
GCACAGAACC GGGCCCAAAA GCTGTTGCAC TACCTCGGCC ATGTCATGGT AAACGGTCCA 
ACCACCCCGA TTCCCGTCAA GGCCAGCCCC AGCCCCACGG ACCCCGTTGT CCCTGCAGTG 
CCCATAGGCC CGCCCCCGGC TGGTTTCAGA GACATCCTGC TGCGAGAGGG GCCTGAGGGC 
TTTGCTCGAG CTGTGCGGAA CCACCCGGGG CTGCTGCTGA TGGACACGAC CTTCAGGGAC 
GCCCACCAGT CACTGCTGGC CACTCGTGTG CGCACCCACG ATCTCAAAAA GATCGCCCCC 
TATGTTGCCC ACAACTTCAG CAAGCTCTTC AGCATGGAGA ACTGGGGAGG AGCCACGTTT 
GACGTCGCCA TGCGCTTCCT GTATGAGTGC CCCTGGCGGC GGCTGCAGGA GCTCCGGGAG 
CTCATCCCCA ACATCCCTTT CCAGATGCTG CTGCGGGGGG CCAATGCTGT GGGCTACACC 
AACTACCCAG ACAACGTGGT CTTCAAGTTC TGTGAAGTGG CCAAAGAGAA TGGCATGGAT 
GTCTTCCGTG TGTTTGACTC CCTCAACTAC TTGCCCAACA TGCTGCTGGG CATGGAGGCG 
GCAGGAAGTG CCGGAGGCGT GGTGGAGGCT GCCATCTCAT ACACGGGCGA CGTGGCCGAC 
CCCAGCCGCA CCAAGTACTC ACTGCAGTAC TACATGGGCT TGGCCGAAGA GCTGGTGCGA 
GCTGGCACCC ACATCCTGTG CATCAAGGAC ATGGCCGGGC TGCTGAAGCC CACGGCCTGC 
ACCATGCTGG TCAGCTCCCT CCGGGACCGC TTCCCCGACC TCCCACTGCA CATCCACACC 
CACGACACGT CAGGGGCAGG CGTGGCAGCC ATGCTGGCCT GTGCCCAGGC TGGAGCTGAT 
GTGGTGGATG TGGCAGCTGA TTCCATGTCT GGGATGACTT CACAGCCCAG CATGGGGGCC 
CTGGTGGCCT GTACCAGAGG GACTCCCCTG GACACAGAGG TGCCCATGGA GCGCGTGTTT 
GACTACAGTG AGTACTGGGA GGGGGCTCGG GGACTGTACG CGGCCTTCGA CTGCACGGCC 
ACCATGAAGT CTGGCAACTC GGACGTGTAT GAAAATGAGA TCCCAGGGGG CCAGTACACC 
AACCTGCACT TCCAGGCCCA CAGCATGGGG CTTGGCTCCA AGTTCAAGGA GGTCAAGAAG 
GCCTATGTGG AGGCCAACCA GATGCTGGGC GATCTCATCA AGGTGACGCC CTCCTCCAAG 
ATCGTGGGGG ACCTGGCCCA GTTTATGGTG CAGAATGGAT TGAGCCGGGC AGAGGCCGAA 
GCTCAGGCGG AAGAGCTGTC CTTTCCCCGC TCCGTGGTGG AGTTCCTGCA GGGCTACATC 
GGTGTCCCCC ATGGGGGGTT CCCCGAACCC TTTCGCTCTA AGGTACTGAA GGACCTGCCA 
AGGGTGGAGG GGCGGCCTGG AGCCTCCCTC CCTCCCCTGG ATCTGCAGGC ACTGGAGAAG 
GAGCTGGTAG ACCGGCATGG GGAGGAGGTG ACGCCGGAAG ATGTGCTCTC AGCAGCTATG 
TACCCCGATG TGTTTGCCCA CTTCAAGGAC TTCACTGCCA CCTTTGGCCC CCTGGATAGC 
CTGAATACTC GCCTCTTCCT GCAGGGACCC AAGATCGCAG AGGAGTTTGA GGTGGAGCTG 
GAGCGGGGCA AGACGCTGCA CATCAAAGCC CTGGCCGTGA GCGACCTGAA CCGGGCCGGC 
CAGAGGCAGG TCTTCTTTGA GCTCAATGGG CAGCTGCGGT CCATCTTGGT CAAGGACACC 
CAGGCCATGA AGGAGATGCA CTTCCACCCC AAGGCCCTAA AGGACGTGAA GGGCCAGATC 
GGGGCGCCCA TGCCTGGGAA GGTGATAGAC ATCAAAGTGG TGGCAGGGGC CAAGGTGGCC 
AAGGGCCAGC CCCTGTGTGT GCTCAGTGCC ATGAAGATGG AGACTGTGGT GACCTCACCC 
ATGGAGGGTA CTGTCCGCAA GGTTCATGTG ACCAAGGACA TGACACTGGA AGGTGACGAC 
CTCATCCTGG AGATCGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mass spectrometry analysis shows the biosynthetic pathways supported by pyruvate carboxylase in highly invasive breast cancer cells.
Biochimica et biophysica acta1863(2)537-551(2017 Feb)
Phannasil P,Ansari IH,El Azzouny M,Longacre MJ,Rattanapornsompong K,Burant CF,MacDonald MJ,Jitrapakdee S

Breast Cancer-Derived Lung Metastases Show Increased Pyruvate Carboxylase-Dependent Anaplerosis.
Cell reports17(3)837-848(2016 10)
Christen S,Lorendeau D,Schmieder R,Broekaert D,Metzger K,Veys K,Elia I,Buescher JM,Orth MF,Davidson SM,Gr?newald TG,De Bock K,Fendt SM

Pyruvate Carboxylase Activates the RIG-I-like Receptor-Mediated Antiviral Immune Response by Targeting the MAVS signalosome.
Scientific reports622002(2016 Feb)
Cao Z,Zhou Y,Zhu S,Feng J,Chen X,Liu S,Peng N,Yang X,Xu G,Zhu Y

Pyruvate carboxylase is critical for non-small-cell lung cancer proliferation.
The Journal of clinical investigation125(2)687-98(2015 Feb)
Sellers K,Fox MP,Bousamra M 2nd,Slone SP,Higashi RM,Miller DM,Wang Y,Yan J,Yuneva MO,Deshpande R,Lane AN,Fan TW

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PC cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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