GPR143 cDNA ORF clone, Homo sapiens(human)

The following GPR143 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPR143 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu106264 XM_024452388.1
Latest version!
Homo sapiens G protein-coupled receptor 143 (GPR143), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu106264 XM_024452387.1
Latest version!
Homo sapiens G protein-coupled receptor 143 (GPR143), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu18715 NM_000273.3
Latest version!
Homo sapiens G protein-coupled receptor 143 (GPR143), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu53925 XM_005274541.3
Latest version!
Homo sapiens G protein-coupled receptor 143 (GPR143), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
View Old Accession Versions >>
OHu18715 NM_000273.2 Homo sapiens G protein-coupled receptor 143 (GPR143), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu106264
    Clone ID Related Accession (Same CDS sequence) XM_024452387.1 , XM_024452388.1
    Accession Version XM_024452387.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product G-protein coupled receptor 143 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGTGATCC GGTCCACCGT GTGGTTAGGA TTCCCAAATT TTGTTGACAG CGTCTCGGAT 
    ATGAACCACA CGGAAATTTG GCCTGCTGCT TTCTGCGTGG GGAGTGCGAT GTGGATCCAG
    CTGTTGTACA GTGCCTGCTT CTGGTGGCTG TTTTGCTATG CAGTGGATGC TTATCTGGTG
    ATCCGGAGAT CGGCAGGACT GAGCACCATC CTGCTGTATC ACATCATGGC GTGGGGCCTG
    GCCACCCTGC TCTGTGTGGA GGGAGCCGCC ATGCTCTACT ACCCTTCCGT GTCCAGGTGT
    GAGCGGGGCC TGGACCACGC CATCCCCCAC TATGTCACCA TGTACCTGCC CCTGCTGCTG
    GTTCTCGTGG CGAACCCCAT CCTGTTCCAA AAGACAGTGA CTGCAGTGGC CTCTTTACTT
    AAAGGAAGAC AAGGCATTTA CACGGAGAAC GAGAGGAGGA TGGGAGCCGT GATCAAGATC
    CGATTTTTCA AAATCATGCT GGTTTTAATT ATTTGTTGGT TGTCGAATAT CATCAATGAA
    AGCCTTTTAT TCTATCTTGA GATGCAAACA GATATCAATG GAGGTTCTTT GAAACCTGTC
    AGAACTGCAG CCAAGACCAC ATGGTTTATT ATGGGAATCC TGAATCCAGC CCAGGGATTT
    CTCTTGTCTT TGGCCTTCTA CGGCTGGACA GGATGCAGCC TGGGTTTTCA GTCTCCCAGG
    AAGGAGATCC AGTGGGAATC ACTGACCACC TCGGCTGCTG AGGGGGCTCA CCCATCCCCA
    CTGATGCCCC ATGAAAACCC TGCTTCCGGG AAGGTGTCTC AAGTGGGTGG GCAGACTTCT
    GACGAAGCCC TGAGCATGCT GTCTGAAGGT TCTGATGCCA GCACAATTGA AATTCACACT
    GCAAGTGAAT CCTGCAACAA AAATGAGGGT GACCCTGCTC TCCCAACCCA TGGAGACCTA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308155.1
    CDS39..1001
    Translation

    Target ORF information:

    RefSeq Version XM_024452387.1
    Organism Homo sapiens(human)
    Definition Homo sapiens G protein-coupled receptor 143 (GPR143), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452387.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGTGATCC GGTCCACCGT GTGGTTAGGA TTCCCAAATT TTGTTGACAG CGTCTCGGAT 
    ATGAACCACA CGGAAATTTG GCCTGCTGCT TTCTGCGTGG GGAGTGCGAT GTGGATCCAG
    CTGTTGTACA GTGCCTGCTT CTGGTGGCTG TTTTGCTATG CAGTGGATGC TTATCTGGTG
    ATCCGGAGAT CGGCAGGACT GAGCACCATC CTGCTGTATC ACATCATGGC GTGGGGCCTG
    GCCACCCTGC TCTGTGTGGA GGGAGCCGCC ATGCTCTACT ACCCTTCCGT GTCCAGGTGT
    GAGCGGGGCC TGGACCACGC CATCCCCCAC TATGTCACCA TGTACCTGCC CCTGCTGCTG
    GTTCTCGTGG CGAACCCCAT CCTGTTCCAA AAGACAGTGA CTGCAGTGGC CTCTTTACTT
    AAAGGAAGAC AAGGCATTTA CACGGAGAAC GAGAGGAGGA TGGGAGCCGT GATCAAGATC
    CGATTTTTCA AAATCATGCT GGTTTTAATT ATTTGTTGGT TGTCGAATAT CATCAATGAA
    AGCCTTTTAT TCTATCTTGA GATGCAAACA GATATCAATG GAGGTTCTTT GAAACCTGTC
    AGAACTGCAG CCAAGACCAC ATGGTTTATT ATGGGAATCC TGAATCCAGC CCAGGGATTT
    CTCTTGTCTT TGGCCTTCTA CGGCTGGACA GGATGCAGCC TGGGTTTTCA GTCTCCCAGG
    AAGGAGATCC AGTGGGAATC ACTGACCACC TCGGCTGCTG AGGGGGCTCA CCCATCCCCA
    CTGATGCCCC ATGAAAACCC TGCTTCCGGG AAGGTGTCTC AAGTGGGTGG GCAGACTTCT
    GACGAAGCCC TGAGCATGCT GTCTGAAGGT TCTGATGCCA GCACAATTGA AATTCACACT
    GCAAGTGAAT CCTGCAACAA AAATGAGGGT GACCCTGCTC TCCCAACCCA TGGAGACCTA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu106264
    Clone ID Related Accession (Same CDS sequence) XM_024452387.1 , XM_024452388.1
    Accession Version XM_024452388.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product G-protein coupled receptor 143 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGTGATCC GGTCCACCGT GTGGTTAGGA TTCCCAAATT TTGTTGACAG CGTCTCGGAT 
    ATGAACCACA CGGAAATTTG GCCTGCTGCT TTCTGCGTGG GGAGTGCGAT GTGGATCCAG
    CTGTTGTACA GTGCCTGCTT CTGGTGGCTG TTTTGCTATG CAGTGGATGC TTATCTGGTG
    ATCCGGAGAT CGGCAGGACT GAGCACCATC CTGCTGTATC ACATCATGGC GTGGGGCCTG
    GCCACCCTGC TCTGTGTGGA GGGAGCCGCC ATGCTCTACT ACCCTTCCGT GTCCAGGTGT
    GAGCGGGGCC TGGACCACGC CATCCCCCAC TATGTCACCA TGTACCTGCC CCTGCTGCTG
    GTTCTCGTGG CGAACCCCAT CCTGTTCCAA AAGACAGTGA CTGCAGTGGC CTCTTTACTT
    AAAGGAAGAC AAGGCATTTA CACGGAGAAC GAGAGGAGGA TGGGAGCCGT GATCAAGATC
    CGATTTTTCA AAATCATGCT GGTTTTAATT ATTTGTTGGT TGTCGAATAT CATCAATGAA
    AGCCTTTTAT TCTATCTTGA GATGCAAACA GATATCAATG GAGGTTCTTT GAAACCTGTC
    AGAACTGCAG CCAAGACCAC ATGGTTTATT ATGGGAATCC TGAATCCAGC CCAGGGATTT
    CTCTTGTCTT TGGCCTTCTA CGGCTGGACA GGATGCAGCC TGGGTTTTCA GTCTCCCAGG
    AAGGAGATCC AGTGGGAATC ACTGACCACC TCGGCTGCTG AGGGGGCTCA CCCATCCCCA
    CTGATGCCCC ATGAAAACCC TGCTTCCGGG AAGGTGTCTC AAGTGGGTGG GCAGACTTCT
    GACGAAGCCC TGAGCATGCT GTCTGAAGGT TCTGATGCCA GCACAATTGA AATTCACACT
    GCAAGTGAAT CCTGCAACAA AAATGAGGGT GACCCTGCTC TCCCAACCCA TGGAGACCTA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308156.1
    CDS26..988
    Translation

    Target ORF information:

    RefSeq Version XM_024452388.1
    Organism Homo sapiens(human)
    Definition Homo sapiens G protein-coupled receptor 143 (GPR143), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452388.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGTGATCC GGTCCACCGT GTGGTTAGGA TTCCCAAATT TTGTTGACAG CGTCTCGGAT 
    ATGAACCACA CGGAAATTTG GCCTGCTGCT TTCTGCGTGG GGAGTGCGAT GTGGATCCAG
    CTGTTGTACA GTGCCTGCTT CTGGTGGCTG TTTTGCTATG CAGTGGATGC TTATCTGGTG
    ATCCGGAGAT CGGCAGGACT GAGCACCATC CTGCTGTATC ACATCATGGC GTGGGGCCTG
    GCCACCCTGC TCTGTGTGGA GGGAGCCGCC ATGCTCTACT ACCCTTCCGT GTCCAGGTGT
    GAGCGGGGCC TGGACCACGC CATCCCCCAC TATGTCACCA TGTACCTGCC CCTGCTGCTG
    GTTCTCGTGG CGAACCCCAT CCTGTTCCAA AAGACAGTGA CTGCAGTGGC CTCTTTACTT
    AAAGGAAGAC AAGGCATTTA CACGGAGAAC GAGAGGAGGA TGGGAGCCGT GATCAAGATC
    CGATTTTTCA AAATCATGCT GGTTTTAATT ATTTGTTGGT TGTCGAATAT CATCAATGAA
    AGCCTTTTAT TCTATCTTGA GATGCAAACA GATATCAATG GAGGTTCTTT GAAACCTGTC
    AGAACTGCAG CCAAGACCAC ATGGTTTATT ATGGGAATCC TGAATCCAGC CCAGGGATTT
    CTCTTGTCTT TGGCCTTCTA CGGCTGGACA GGATGCAGCC TGGGTTTTCA GTCTCCCAGG
    AAGGAGATCC AGTGGGAATC ACTGACCACC TCGGCTGCTG AGGGGGCTCA CCCATCCCCA
    CTGATGCCCC ATGAAAACCC TGCTTCCGGG AAGGTGTCTC AAGTGGGTGG GCAGACTTCT
    GACGAAGCCC TGAGCATGCT GTCTGAAGGT TCTGATGCCA GCACAATTGA AATTCACACT
    GCAAGTGAAT CCTGCAACAA AAATGAGGGT GACCCTGCTC TCCCAACCCA TGGAGACCTA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18715
    Clone ID Related Accession (Same CDS sequence) NM_000273.3 , NM_000273.2
    Accession Version NM_000273.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product G-protein coupled receptor 143
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z48804.1, BU188283.1 and AC003036.1. This sequence is a reference standard in the RefSeqGene project. On Nov 24, 2018 this sequence version replaced NM_000273.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC068977.1, Z48804.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000467482.6/ ENSP00000417161.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCCTCCC CGCGCCTAGG GACCTTCTGC TGCCCCACGC GGGACGCAGC CACGCAGCTC 
    GTGCTGAGCT TCCAGCCGCG GGCCTTCCAC GCGCTCTGCC TGGGCAGCGG CGGGCTCCGC
    TTGGCGCTGG GCCTTCTGCA GCTGCTGCCC GGCCGCCGGC CCGCGGGCCC CGGGTCCCCC
    GCGACGTCCC CGCCGGCCTC GGTCCGCATC CTGCGCGCTG CCGCTGCCTG CGACCTTCTC
    GGCTGCCTGG GTATGGTGAT CCGGTCCACC GTGTGGTTAG GATTCCCAAA TTTTGTTGAC
    AGCGTCTCGG ATATGAACCA CACGGAAATT TGGCCTGCTG CTTTCTGCGT GGGGAGTGCG
    ATGTGGATCC AGCTGTTGTA CAGTGCCTGC TTCTGGTGGC TGTTTTGCTA TGCAGTGGAT
    GCTTATCTGG TGATCCGGAG ATCGGCAGGA CTGAGCACCA TCCTGCTGTA TCACATCATG
    GCGTGGGGCC TGGCCACCCT GCTCTGTGTG GAGGGAGCCG CCATGCTCTA CTACCCTTCC
    GTGTCCAGGT GTGAGCGGGG CCTGGACCAC GCCATCCCCC ACTATGTCAC CATGTACCTG
    CCCCTGCTGC TGGTTCTCGT GGCGAACCCC ATCCTGTTCC AAAAGACAGT GACTGCAGTG
    GCCTCTTTAC TTAAAGGAAG ACAAGGCATT TACACGGAGA ACGAGAGGAG GATGGGAGCC
    GTGATCAAGA TCCGATTTTT CAAAATCATG CTGGTTTTAA TTATTTGTTG GTTGTCGAAT
    ATCATCAATG AAAGCCTTTT ATTCTATCTT GAGATGCAAA CAGATATCAA TGGAGGTTCT
    TTGAAACCTG TCAGAACTGC AGCCAAGACC ACATGGTTTA TTATGGGAAT CCTGAATCCA
    GCCCAGGGAT TTCTCTTGTC TTTGGCCTTC TACGGCTGGA CAGGATGCAG CCTGGGTTTT
    CAGTCTCCCA GGAAGGAGAT CCAGTGGGAA TCACTGACCA CCTCGGCTGC TGAGGGGGCT
    CACCCATCCC CACTGATGCC CCATGAAAAC CCTGCTTCCG GGAAGGTGTC TCAAGTGGGT
    GGGCAGACTT CTGACGAAGC CCTGAGCATG CTGTCTGAAG GTTCTGATGC CAGCACAATT
    GAAATTCACA CTGCAAGTGA ATCCTGCAAC AAAAATGAGG GTGACCCTGC TCTCCCAACC
    CATGGAGACC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000264.2
    CDS31..1245
    Translation

    Target ORF information:

    RefSeq Version NM_000273.3
    Organism Homo sapiens(human)
    Definition Homo sapiens G protein-coupled receptor 143 (GPR143), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000273.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCCTCCC CGCGCCTAGG GACCTTCTGC TGCCCCACGC GGGACGCAGC CACGCAGCTC 
    GTGCTGAGCT TCCAGCCGCG GGCCTTCCAC GCGCTCTGCC TGGGCAGCGG CGGGCTCCGC
    TTGGCGCTGG GCCTTCTGCA GCTGCTGCCC GGCCGCCGGC CCGCGGGCCC CGGGTCCCCC
    GCGACGTCCC CGCCGGCCTC GGTCCGCATC CTGCGCGCTG CCGCTGCCTG CGACCTTCTC
    GGCTGCCTGG GTATGGTGAT CCGGTCCACC GTGTGGTTAG GATTCCCAAA TTTTGTTGAC
    AGCGTCTCGG ATATGAACCA CACGGAAATT TGGCCTGCTG CTTTCTGCGT GGGGAGTGCG
    ATGTGGATCC AGCTGTTGTA CAGTGCCTGC TTCTGGTGGC TGTTTTGCTA TGCAGTGGAT
    GCTTATCTGG TGATCCGGAG ATCGGCAGGA CTGAGCACCA TCCTGCTGTA TCACATCATG
    GCGTGGGGCC TGGCCACCCT GCTCTGTGTG GAGGGAGCCG CCATGCTCTA CTACCCTTCC
    GTGTCCAGGT GTGAGCGGGG CCTGGACCAC GCCATCCCCC ACTATGTCAC CATGTACCTG
    CCCCTGCTGC TGGTTCTCGT GGCGAACCCC ATCCTGTTCC AAAAGACAGT GACTGCAGTG
    GCCTCTTTAC TTAAAGGAAG ACAAGGCATT TACACGGAGA ACGAGAGGAG GATGGGAGCC
    GTGATCAAGA TCCGATTTTT CAAAATCATG CTGGTTTTAA TTATTTGTTG GTTGTCGAAT
    ATCATCAATG AAAGCCTTTT ATTCTATCTT GAGATGCAAA CAGATATCAA TGGAGGTTCT
    TTGAAACCTG TCAGAACTGC AGCCAAGACC ACATGGTTTA TTATGGGAAT CCTGAATCCA
    GCCCAGGGAT TTCTCTTGTC TTTGGCCTTC TACGGCTGGA CAGGATGCAG CCTGGGTTTT
    CAGTCTCCCA GGAAGGAGAT CCAGTGGGAA TCACTGACCA CCTCGGCTGC TGAGGGGGCT
    CACCCATCCC CACTGATGCC CCATGAAAAC CCTGCTTCCG GGAAGGTGTC TCAAGTGGGT
    GGGCAGACTT CTGACGAAGC CCTGAGCATG CTGTCTGAAG GTTCTGATGC CAGCACAATT
    GAAATTCACA CTGCAAGTGA ATCCTGCAAC AAAAATGAGG GTGACCCTGC TCTCCCAACC
    CATGGAGACC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18715
    Clone ID Related Accession (Same CDS sequence) NM_000273.3 , NM_000273.2
    Accession Version NM_000273.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-11-11
    Organism Homo sapiens(human)
    Product G-protein coupled receptor 143
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC068977.1, Z48804.1 and BU188283.1. This sequence is a reference standard in the RefSeqGene project. On Dec 4, 2009 this sequence version replaced NM_000273.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC068977.1, Z48804.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: community standard (PMID:11793467) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCCTCCC CGCGCCTAGG GACCTTCTGC TGCCCCACGC GGGACGCAGC CACGCAGCTC 
    GTGCTGAGCT TCCAGCCGCG GGCCTTCCAC GCGCTCTGCC TGGGCAGCGG CGGGCTCCGC
    TTGGCGCTGG GCCTTCTGCA GCTGCTGCCC GGCCGCCGGC CCGCGGGCCC CGGGTCCCCC
    GCGACGTCCC CGCCGGCCTC GGTCCGCATC CTGCGCGCTG CCGCTGCCTG CGACCTTCTC
    GGCTGCCTGG GTATGGTGAT CCGGTCCACC GTGTGGTTAG GATTCCCAAA TTTTGTTGAC
    AGCGTCTCGG ATATGAACCA CACGGAAATT TGGCCTGCTG CTTTCTGCGT GGGGAGTGCG
    ATGTGGATCC AGCTGTTGTA CAGTGCCTGC TTCTGGTGGC TGTTTTGCTA TGCAGTGGAT
    GCTTATCTGG TGATCCGGAG ATCGGCAGGA CTGAGCACCA TCCTGCTGTA TCACATCATG
    GCGTGGGGCC TGGCCACCCT GCTCTGTGTG GAGGGAGCCG CCATGCTCTA CTACCCTTCC
    GTGTCCAGGT GTGAGCGGGG CCTGGACCAC GCCATCCCCC ACTATGTCAC CATGTACCTG
    CCCCTGCTGC TGGTTCTCGT GGCGAACCCC ATCCTGTTCC AAAAGACAGT GACTGCAGTG
    GCCTCTTTAC TTAAAGGAAG ACAAGGCATT TACACGGAGA ACGAGAGGAG GATGGGAGCC
    GTGATCAAGA TCCGATTTTT CAAAATCATG CTGGTTTTAA TTATTTGTTG GTTGTCGAAT
    ATCATCAATG AAAGCCTTTT ATTCTATCTT GAGATGCAAA CAGATATCAA TGGAGGTTCT
    TTGAAACCTG TCAGAACTGC AGCCAAGACC ACATGGTTTA TTATGGGAAT CCTGAATCCA
    GCCCAGGGAT TTCTCTTGTC TTTGGCCTTC TACGGCTGGA CAGGATGCAG CCTGGGTTTT
    CAGTCTCCCA GGAAGGAGAT CCAGTGGGAA TCACTGACCA CCTCGGCTGC TGAGGGGGCT
    CACCCATCCC CACTGATGCC CCATGAAAAC CCTGCTTCCG GGAAGGTGTC TCAAGTGGGT
    GGGCAGACTT CTGACGAAGC CCTGAGCATG CTGTCTGAAG GTTCTGATGC CAGCACAATT
    GAAATTCACA CTGCAAGTGA ATCCTGCAAC AAAAATGAGG GTGACCCTGC TCTCCCAACC
    CATGGAGACC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000264.2
    CDS149..1363
    Translation

    Target ORF information:

    RefSeq Version NM_000273.2
    Organism Homo sapiens(human)
    Definition Homo sapiens G protein-coupled receptor 143 (GPR143), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000273.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCCTCCC CGCGCCTAGG GACCTTCTGC TGCCCCACGC GGGACGCAGC CACGCAGCTC 
    GTGCTGAGCT TCCAGCCGCG GGCCTTCCAC GCGCTCTGCC TGGGCAGCGG CGGGCTCCGC
    TTGGCGCTGG GCCTTCTGCA GCTGCTGCCC GGCCGCCGGC CCGCGGGCCC CGGGTCCCCC
    GCGACGTCCC CGCCGGCCTC GGTCCGCATC CTGCGCGCTG CCGCTGCCTG CGACCTTCTC
    GGCTGCCTGG GTATGGTGAT CCGGTCCACC GTGTGGTTAG GATTCCCAAA TTTTGTTGAC
    AGCGTCTCGG ATATGAACCA CACGGAAATT TGGCCTGCTG CTTTCTGCGT GGGGAGTGCG
    ATGTGGATCC AGCTGTTGTA CAGTGCCTGC TTCTGGTGGC TGTTTTGCTA TGCAGTGGAT
    GCTTATCTGG TGATCCGGAG ATCGGCAGGA CTGAGCACCA TCCTGCTGTA TCACATCATG
    GCGTGGGGCC TGGCCACCCT GCTCTGTGTG GAGGGAGCCG CCATGCTCTA CTACCCTTCC
    GTGTCCAGGT GTGAGCGGGG CCTGGACCAC GCCATCCCCC ACTATGTCAC CATGTACCTG
    CCCCTGCTGC TGGTTCTCGT GGCGAACCCC ATCCTGTTCC AAAAGACAGT GACTGCAGTG
    GCCTCTTTAC TTAAAGGAAG ACAAGGCATT TACACGGAGA ACGAGAGGAG GATGGGAGCC
    GTGATCAAGA TCCGATTTTT CAAAATCATG CTGGTTTTAA TTATTTGTTG GTTGTCGAAT
    ATCATCAATG AAAGCCTTTT ATTCTATCTT GAGATGCAAA CAGATATCAA TGGAGGTTCT
    TTGAAACCTG TCAGAACTGC AGCCAAGACC ACATGGTTTA TTATGGGAAT CCTGAATCCA
    GCCCAGGGAT TTCTCTTGTC TTTGGCCTTC TACGGCTGGA CAGGATGCAG CCTGGGTTTT
    CAGTCTCCCA GGAAGGAGAT CCAGTGGGAA TCACTGACCA CCTCGGCTGC TGAGGGGGCT
    CACCCATCCC CACTGATGCC CCATGAAAAC CCTGCTTCCG GGAAGGTGTC TCAAGTGGGT
    GGGCAGACTT CTGACGAAGC CCTGAGCATG CTGTCTGAAG GTTCTGATGC CAGCACAATT
    GAAATTCACA CTGCAAGTGA ATCCTGCAAC AAAAATGAGG GTGACCCTGC TCTCCCAACC
    CATGGAGACC TATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53925
    Clone ID Related Accession (Same CDS sequence) XM_005274541.3
    Accession Version XM_005274541.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product G-protein coupled receptor 143 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005274541.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCCTCCC CGCGCCTAGG GACCTTCTGC TGCCCCACGC GGGACGCAGC CACGCAGCTC 
    GTGCTGAGCT TCCAGCCGCG GGCCTTCCAC GCGCTCTGCC TGGGCAGCGG CGGGCTCCGC
    TTGGCGCTGG GCCTTCTGCA GCTGCTGCCC GGCCGCCGGC CCGCGGGCCC CGGGTCCCCC
    GCGACGTCCC CGCCGGCCTC GGTCCGCATC CTGCGCGCTG CCGCTGCCTG CGACCTTCTC
    GGCTGCCTGG GTATGGTGAT CCGGTCCACC GTGTGGTTAG GATTCCCAAA TTTTGTTGAC
    AGCGTCTCGG ATATGAACCA CACGGAAATT TGGCCTGCTG CTTTCTGCGT GGGGAGTGCG
    ATGTGGATCC AGCTGTTGTA CAGTGCCTGC TTCTGGTGGC TGTTTTGCTA TGCAGTGGAT
    GCTTATCTGG TGATCCGGAG ATCGGCAGGA CTGAGCACCA TCCTGCTGTA TCACATCATG
    GCGTGGGGCC TGGCCACCCT GCTCTGTGTG GAGGGAGCCG CCATGCTCTA CTACCCTTCC
    GTGTCCAGGT GTGAGCGGGG CCTGGACCAC GCCATCCCCC ACTATGTCAC CATGTACCTG
    CCCCTGCTGC TGGTTCTCGT GGCGAACCCC ATCCTGTTCC AAAAGACAGT GACTGCAGTG
    GCCTCTTTAC TTAAAGGAAG ACAAGGCATT TACACGGAGA ACGAGAGGAG GATGGGAGCC
    GTGATCAAGA TCCGATTTTT CAAAATCATG CTGGTTTTAA TTATTTGTTG GTTGTCGAAT
    ATCATCAATG AAAGCCTTTT ATTCTATCTT GAGATGCAAA CAGATATCAA TGGAGGTTCT
    TTGAAACCTG TCAGAACTGC AGCCAAGACC ACATGGTTTA TTATGGGAAT CCTGAATCCA
    GCCCAGGGAT TTCTCTTGTC TTTGGCCTTC TACGGCTGGA CAGGATGCAG CCTGGGTTTT
    CAGTCTCCCA GGAAGGAGAT CCAGTGGGAA TCACTGACCA CCTCGGCTGC TGAGGGGGCT
    CACCCATCCC CACTGATGCC CCATGAAAAC CCTGCTTCCG GGAAGGTGTC TCAAGTGGGT
    GGGCAGACTT CTGACGAAGC CCTGAGCATG CTGTCTGAAG ATGAGATGAA TGCTAGGACT
    TTGACCAAAG TTGGTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005274598.1
    CDS27..1187
    Translation

    Target ORF information:

    RefSeq Version XM_005274541.3
    Organism Homo sapiens(human)
    Definition Homo sapiens G protein-coupled receptor 143 (GPR143), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005274541.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCCTCCC CGCGCCTAGG GACCTTCTGC TGCCCCACGC GGGACGCAGC CACGCAGCTC 
    GTGCTGAGCT TCCAGCCGCG GGCCTTCCAC GCGCTCTGCC TGGGCAGCGG CGGGCTCCGC
    TTGGCGCTGG GCCTTCTGCA GCTGCTGCCC GGCCGCCGGC CCGCGGGCCC CGGGTCCCCC
    GCGACGTCCC CGCCGGCCTC GGTCCGCATC CTGCGCGCTG CCGCTGCCTG CGACCTTCTC
    GGCTGCCTGG GTATGGTGAT CCGGTCCACC GTGTGGTTAG GATTCCCAAA TTTTGTTGAC
    AGCGTCTCGG ATATGAACCA CACGGAAATT TGGCCTGCTG CTTTCTGCGT GGGGAGTGCG
    ATGTGGATCC AGCTGTTGTA CAGTGCCTGC TTCTGGTGGC TGTTTTGCTA TGCAGTGGAT
    GCTTATCTGG TGATCCGGAG ATCGGCAGGA CTGAGCACCA TCCTGCTGTA TCACATCATG
    GCGTGGGGCC TGGCCACCCT GCTCTGTGTG GAGGGAGCCG CCATGCTCTA CTACCCTTCC
    GTGTCCAGGT GTGAGCGGGG CCTGGACCAC GCCATCCCCC ACTATGTCAC CATGTACCTG
    CCCCTGCTGC TGGTTCTCGT GGCGAACCCC ATCCTGTTCC AAAAGACAGT GACTGCAGTG
    GCCTCTTTAC TTAAAGGAAG ACAAGGCATT TACACGGAGA ACGAGAGGAG GATGGGAGCC
    GTGATCAAGA TCCGATTTTT CAAAATCATG CTGGTTTTAA TTATTTGTTG GTTGTCGAAT
    ATCATCAATG AAAGCCTTTT ATTCTATCTT GAGATGCAAA CAGATATCAA TGGAGGTTCT
    TTGAAACCTG TCAGAACTGC AGCCAAGACC ACATGGTTTA TTATGGGAAT CCTGAATCCA
    GCCCAGGGAT TTCTCTTGTC TTTGGCCTTC TACGGCTGGA CAGGATGCAG CCTGGGTTTT
    CAGTCTCCCA GGAAGGAGAT CCAGTGGGAA TCACTGACCA CCTCGGCTGC TGAGGGGGCT
    CACCCATCCC CACTGATGCC CCATGAAAAC CCTGCTTCCG GGAAGGTGTC TCAAGTGGGT
    GGGCAGACTT CTGACGAAGC CCTGAGCATG CTGTCTGAAG ATGAGATGAA TGCTAGGACT
    TTGACCAAAG TTGGTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.