NRAS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NRAS
Entrez Gene ID	4893
Full Name	NRAS proto-oncogene, GTPase
Synonyms	ALPS4,CMNS,N-ras,NCMS,NRAS1,NS6
General protein information	Preferred Names NRAS proto-oncogene, GTPase Names GTPase NRas N-ras protein part 4 neuroblastoma RAS viral (v-ras) oncogene homolog neuroblastoma RAS viral oncogene homolog transforming protein N-Ras v-ras neuroblastoma RAS viral oncogene homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p13.2
Summary	This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011].
Disorder MIM:	164790
Disorder Html:	?RAS-associated autoimmune lymphoproliferative syndrome type IV, somatic, 614470 (3); Noonan syndrome 6, 613224 (3); Epidermal nevus, somatic, 162900 (3); Thyroid carcinoma, follicular, somatic, 188470 (3); Colorectal cancer, somatic, 114500 (3); Melanocytic nevus syndrome, congenital, somatic, 137550 (3); Neurocutaneous melanosis, somatic, 249400 (3); Schimmelpenning-Feuerstein-Mims syndrome, somatic mosaic, 163200 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002524.4	NP_002515.1	GTPase NRas
NM_002524.5	NP_002515.1	GTPase NRas

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nras	NP_542944.1
Xenopus tropicalis (tropical clawed frog)	LOC549517	NP_001016763.1
Canis lupus familiaris (dog)	NRAS	XP_848629.1
Mus musculus (house mouse)	Nras	NP_035067.2
Gallus gallus (chicken)	NRAS	NP_001012567.1
Homo sapiens (human)	NRAS	NP_002515.1
Pan troglodytes (chimpanzee)	NRAS	XP_001149822.1
Bos taurus (cattle)	NRAS	NP_001091458.1
Danio rerio (zebrafish)	nras	NP_571220.1

Gene Ontology
Bibliography

Related articles in PubMed

Ponti G, Manfredini M, Greco S, Pellacani G, Depenni R, Tomasi A, Maccaferri M, Cascinu S
Anticancer research37(12)7043-7048(2017 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutational status of NRAS, KRAS, and PTPN11 genes is associated with genetic/cytogenetic features in children with B-precursor acute lymphoblastic leukemia.
Title: Mutational status of NRAS, KRAS, and PTPN11 genes is associated with genetic/cytogenetic features in children with B-precursor acute lymphoblastic leukemia.

Data indicate that BRAF, NRAS and C-KIT melanomas constitute distinct clinico-pathological entities.

KIT knockdown increased RAS/MAPK pathway activation in a BRAF(V600E)-mutant melanoma cell line.
Title: KIT Suppresses BRAF

BRAF and NRAS mutations were associated with CNS and liver metastasis and NRAS mutation with lung metastasis.
Title: Tumour mutation status and sites of metastasis in patients with cutaneous melanoma.

NRAS mutations were present in 1.2% of primary papillary thyroid carcinomas, 1.3% of lymph node metastasis, and 14.3% of distant metastasis.
Title: TERT, BRAF, and NRAS in Primary Thyroid Cancer and Metastatic Disease.

The following NRAS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NRAS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17762	NM_002524.5 Latest version!	Homo sapiens NRAS proto-oncogene, GTPase (NRAS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OHu17762	NM_002524.4	Homo sapiens NRAS proto-oncogene, GTPase (NRAS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NRAS ( NM_002524.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002515.1 Homo sapiens NRAS proto-oncogene, GTPase (NRAS), mRNA.

Price & Availability↑

CloneID	OHu17762
Clone ID Related Accession (Same CDS sequence)	NM_002524.4 , NM_002524.5
Accession Version	NM_002524.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	GTPase NRas
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL096773.6, BC005219.1, AY941101.1, BC013214.1 and AW021410.1. On Jun 2, 2011 this sequence version replaced NM_002524.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.246845.1, SRR1803615.226704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267772 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGACTGAGT ACAAACTGGT GGTGGTTGGA GCAGGTGGTG TTGGGAAAAG CGCACTGACA 
ATCCAGCTAA TCCAGAACCA CTTTGTAGAT GAATATGATC CCACCATAGA GGATTCTTAC 
AGAAAACAAG TGGTTATAGA TGGTGAAACC TGTTTGTTGG ACATACTGGA TACAGCTGGA 
CAAGAAGAGT ACAGTGCCAT GAGAGACCAA TACATGAGGA CAGGCGAAGG CTTCCTCTGT 
GTATTTGCCA TCAATAATAG CAAGTCATTT GCGGATATTA ACCTCTACAG GGAGCAGATT 
AAGCGAGTAA AAGACTCGGA TGATGTACCT ATGGTGCTAG TGGGAAACAA GTGTGATTTG 
CCAACAAGGA CAGTTGATAC AAAACAAGCC CACGAACTGG CCAAGAGTTA CGGGATTCCA 
TTCATTGAAA CCTCAGCCAA GACCAGACAG GGTGTTGAAG ATGCTTTTTA CACACTGGTA 
AGAGAAATAC GCCAGTACCG AATGAAAAAA CTCAACAGCA GTGATGATGG GACTCAGGGT 
TGTATGGGAT TGCCATGTGT GGTGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002515.1
CDS	255..824
Translation	MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRMKKLNSSDDGTQGCMGLPCVVM

Target ORF information:

RefSeq Version	NM_002524.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NRAS proto-oncogene, GTPase (NRAS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002524.4

ORF Insert Sequence:

ATGACTGAGT ACAAACTGGT GGTGGTTGGA GCAGGTGGTG TTGGGAAAAG CGCACTGACA 
ATCCAGCTAA TCCAGAACCA CTTTGTAGAT GAATATGATC CCACCATAGA GGATTCTTAC 
AGAAAACAAG TGGTTATAGA TGGTGAAACC TGTTTGTTGG ACATACTGGA TACAGCTGGA 
CAAGAAGAGT ACAGTGCCAT GAGAGACCAA TACATGAGGA CAGGCGAAGG CTTCCTCTGT 
GTATTTGCCA TCAATAATAG CAAGTCATTT GCGGATATTA ACCTCTACAG GGAGCAGATT 
AAGCGAGTAA AAGACTCGGA TGATGTACCT ATGGTGCTAG TGGGAAACAA GTGTGATTTG 
CCAACAAGGA CAGTTGATAC AAAACAAGCC CACGAACTGG CCAAGAGTTA CGGGATTCCA 
TTCATTGAAA CCTCAGCCAA GACCAGACAG GGTGTTGAAG ATGCTTTTTA CACACTGGTA 
AGAGAAATAC GCCAGTACCG AATGAAAAAA CTCAACAGCA GTGATGATGG GACTCAGGGT 
TGTATGGGAT TGCCATGTGT GGTGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRAS ( NM_002524.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002515.1 Homo sapiens NRAS proto-oncogene, GTPase (NRAS), mRNA.

Price & Availability↑

CloneID	OHu17762
Clone ID Related Accession (Same CDS sequence)	NM_002524.4 , NM_002524.5
Accession Version	NM_002524.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Homo sapiens(human)
Product	GTPase NRas
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL096773.6, BC005219.1, AY941101.1, BC013214.1 and AW021410.1. On Nov 22, 2018 this sequence version replaced NM_002524.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.226704.1, SRR1660803.246845.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267772 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000369535.5/ ENSP00000358548.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACTGAGT ACAAACTGGT GGTGGTTGGA GCAGGTGGTG TTGGGAAAAG CGCACTGACA 
ATCCAGCTAA TCCAGAACCA CTTTGTAGAT GAATATGATC CCACCATAGA GGATTCTTAC 
AGAAAACAAG TGGTTATAGA TGGTGAAACC TGTTTGTTGG ACATACTGGA TACAGCTGGA 
CAAGAAGAGT ACAGTGCCAT GAGAGACCAA TACATGAGGA CAGGCGAAGG CTTCCTCTGT 
GTATTTGCCA TCAATAATAG CAAGTCATTT GCGGATATTA ACCTCTACAG GGAGCAGATT 
AAGCGAGTAA AAGACTCGGA TGATGTACCT ATGGTGCTAG TGGGAAACAA GTGTGATTTG 
CCAACAAGGA CAGTTGATAC AAAACAAGCC CACGAACTGG CCAAGAGTTA CGGGATTCCA 
TTCATTGAAA CCTCAGCCAA GACCAGACAG GGTGTTGAAG ATGCTTTTTA CACACTGGTA 
AGAGAAATAC GCCAGTACCG AATGAAAAAA CTCAACAGCA GTGATGATGG GACTCAGGGT 
TGTATGGGAT TGCCATGTGT GGTGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002515.1
CDS	132..701
Translation	MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRMKKLNSSDDGTQGCMGLPCVVM

Target ORF information:

RefSeq Version	NM_002524.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NRAS proto-oncogene, GTPase (NRAS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002524.5

ORF Insert Sequence:

ATGACTGAGT ACAAACTGGT GGTGGTTGGA GCAGGTGGTG TTGGGAAAAG CGCACTGACA 
ATCCAGCTAA TCCAGAACCA CTTTGTAGAT GAATATGATC CCACCATAGA GGATTCTTAC 
AGAAAACAAG TGGTTATAGA TGGTGAAACC TGTTTGTTGG ACATACTGGA TACAGCTGGA 
CAAGAAGAGT ACAGTGCCAT GAGAGACCAA TACATGAGGA CAGGCGAAGG CTTCCTCTGT 
GTATTTGCCA TCAATAATAG CAAGTCATTT GCGGATATTA ACCTCTACAG GGAGCAGATT 
AAGCGAGTAA AAGACTCGGA TGATGTACCT ATGGTGCTAG TGGGAAACAA GTGTGATTTG 
CCAACAAGGA CAGTTGATAC AAAACAAGCC CACGAACTGG CCAAGAGTTA CGGGATTCCA 
TTCATTGAAA CCTCAGCCAA GACCAGACAG GGTGTTGAAG ATGCTTTTTA CACACTGGTA 
AGAGAAATAC GCCAGTACCG AATGAAAAAA CTCAACAGCA GTGATGATGG GACTCAGGGT 
TGTATGGGAT TGCCATGTGT GGTGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Anticancer research37(12)7043-7048(2017 12)
Ponti G,Manfredini M,Greco S,Pellacani G,Depenni R,Tomasi A,Maccaferri M,Cascinu S

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NRAS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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