NPC1 cDNA ORF clone, Homo sapiens(human)

The following NPC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu87898 XM_017025784.1
Latest version!
Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu87899 XM_017025785.1
Latest version!
Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu87900 XM_017025786.1
Latest version!
Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu87901 XM_017025787.1
Latest version!
Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu27327 NM_000271.5
Latest version!
Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu51216 XM_005258277.1
Latest version!
Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu51217 XM_005258278.5
Latest version!
Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu51218 XM_005258279.2
Latest version!
Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu51219 XM_006722479.3
Latest version!
Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu59314 XM_011526015.1
Latest version!
Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu27327 NM_000271.4 Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $384.30
$549.00
OHu51217 XM_005258278.4 Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu87898
    Clone ID Related Accession (Same CDS sequence) XM_017025784.1
    Accession Version XM_017025784.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product NPC intracellular cholesterol transporter 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
    GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
    TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
    TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
    TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
    CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
    CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
    CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
    CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
    CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
    GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
    TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
    AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
    TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
    ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
    GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
    ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
    GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
    AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
    GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
    CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
    ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
    CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
    GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
    GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
    GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
    GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
    TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
    GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
    CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
    CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
    GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
    TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
    GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
    GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
    CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
    TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
    AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
    GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
    AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
    TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
    GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
    GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
    CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
    TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
    GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
    CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
    TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
    CTCCCTGTCT TACTCAGTTA CATAGATGAT TTGAAGGTAC CTGTACTGCA ATCTGAAACT
    GGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881273.1
    CDS164..4009
    Translation

    Target ORF information:

    RefSeq Version XM_017025784.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
    GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
    TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
    TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
    TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
    CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
    CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
    CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
    CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
    CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
    GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
    TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
    AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
    TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
    ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
    GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
    ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
    GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
    AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
    GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
    CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
    ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
    CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
    GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
    GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
    GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
    GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
    TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
    GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
    CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
    CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
    GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
    TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
    GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
    GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
    CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
    TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
    AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
    GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
    AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
    TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
    GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
    GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
    CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
    TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
    GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
    CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
    TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
    CTCCCTGTCT TACTCAGTTA CATAGATGAT TTGAAGGTAC CTGTACTGCA ATCTGAAACT
    GGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87899
    Clone ID Related Accession (Same CDS sequence) XM_017025785.1
    Accession Version XM_017025785.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product NPC intracellular cholesterol transporter 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
    GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
    TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
    TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
    TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
    CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
    CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
    CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
    CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
    CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
    GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
    TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
    AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
    TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
    ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
    GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
    ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
    GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
    AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
    GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
    CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
    ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
    CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
    GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
    GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
    GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
    GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
    TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
    GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
    CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
    CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
    GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
    TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
    GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
    GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
    CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
    TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
    AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
    GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
    AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
    TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
    GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
    GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
    CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
    TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
    GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
    CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
    TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
    CTCCCTGTCT TACTCAGTTA CATAGCAACT TCCTTGTTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881274.1
    CDS164..3985
    Translation

    Target ORF information:

    RefSeq Version XM_017025785.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025785.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
    GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
    TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
    TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
    TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
    CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
    CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
    CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
    CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
    CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
    GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
    TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
    AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
    TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
    ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
    GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
    ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
    GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
    AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
    GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
    CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
    ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
    CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
    GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
    GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
    GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
    GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
    TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
    GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
    CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
    CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
    GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
    TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
    GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
    GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
    CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
    TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
    AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
    GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
    AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
    TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
    GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
    GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
    CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
    TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
    GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
    CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
    TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
    CTCCCTGTCT TACTCAGTTA CATAGCAACT TCCTTGTTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87900
    Clone ID Related Accession (Same CDS sequence) XM_017025786.1
    Accession Version XM_017025786.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product NPC intracellular cholesterol transporter 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
    GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
    CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
    ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
    TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
    CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
    CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
    CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
    ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
    ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
    GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
    GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
    GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
    CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
    CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
    ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
    GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
    CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
    ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
    CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
    AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
    GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
    TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
    TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
    AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
    GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
    TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
    ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
    GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
    CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
    TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
    CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
    TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
    AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
    CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
    ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
    TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
    TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
    ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
    TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
    AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
    CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
    GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
    TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
    GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
    GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
    TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
    TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGATGATT TGAAGGTACC TGTACTGCAA
    TCTGAAACTG GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881275.1
    CDS164..3958
    Translation

    Target ORF information:

    RefSeq Version XM_017025786.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025786.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
    GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
    CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
    ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
    TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
    CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
    CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
    CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
    ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
    ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
    GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
    GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
    GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
    CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
    CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
    ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
    GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
    CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
    ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
    CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
    AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
    GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
    TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
    TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
    AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
    GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
    TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
    ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
    GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
    CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
    TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
    CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
    TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
    AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
    CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
    ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
    TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
    TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
    ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
    TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
    AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
    CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
    GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
    TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
    GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
    GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
    TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
    TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGATGATT TGAAGGTACC TGTACTGCAA
    TCTGAAACTG GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu87901
    Clone ID Related Accession (Same CDS sequence) XM_017025787.1
    Accession Version XM_017025787.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3771bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product NPC intracellular cholesterol transporter 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
    GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
    CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
    ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
    TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
    CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
    CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
    CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
    ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
    ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
    GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
    GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
    GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
    CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
    CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
    ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
    GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
    CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
    ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
    CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
    AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
    GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
    TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
    TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
    AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
    GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
    TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
    ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
    GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
    CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
    TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
    CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
    TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
    AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
    CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
    ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
    TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
    TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
    ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
    TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
    AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
    CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
    GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
    TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
    GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
    GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
    TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
    TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGGCCCAT TCAATTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016881276.1
    CDS164..3934
    Translation

    Target ORF information:

    RefSeq Version XM_017025787.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017025787.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
    GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
    CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
    ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
    TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
    CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
    CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
    CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
    ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
    ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
    GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
    GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
    GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
    CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
    CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
    ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
    GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
    CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
    ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
    CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
    AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
    GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
    TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
    TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
    AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
    GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
    TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
    ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
    GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
    CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
    TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
    CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
    TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
    AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
    CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
    ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
    TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
    TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
    ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
    TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
    AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
    CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
    GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
    TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
    GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
    GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
    TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
    TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGGCCCAT TCAATTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27327
    Clone ID Related Accession (Same CDS sequence) NM_000271.5 , NM_000271.4
    Accession Version NM_000271.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3837bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Homo sapiens(human)
    Product NPC intracellular cholesterol transporter 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR159421.1, AF002020.1, AB209048.1, BC063302.1 and AC010853.11. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000271.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.10452.1, SRR1803611.59216.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000269228.10/ ENSP00000269228.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
    GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
    CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
    ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
    TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
    CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
    CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
    CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
    ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
    ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
    GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
    GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
    GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
    CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
    CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
    ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
    GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
    CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
    ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
    CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
    AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
    GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
    TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
    TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
    AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
    GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
    TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
    ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
    GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
    CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
    TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
    CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
    TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
    AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
    CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
    ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
    TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
    TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
    ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
    TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
    AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
    CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
    GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
    TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
    GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
    GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
    TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
    TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGGGCCAT CAGTAAATAA AGCCAAAAGT
    TGTGCCACTG AAGAGCGATA CAAAGGAACA GAGCGCGAAC GGCTTCTAAA TTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000262.2
    CDS164..4000
    Translation

    Target ORF information:

    RefSeq Version NM_000271.5
    Organism Homo sapiens(human)
    Definition Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000271.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
    GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
    CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
    ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
    TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
    CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
    CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
    CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
    ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
    ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
    GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
    GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
    GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
    CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
    CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
    ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
    GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
    CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
    ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
    CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
    AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
    GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
    TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
    TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
    AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
    GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
    TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
    ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
    GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
    CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
    TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
    CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
    TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
    AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
    CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
    ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
    TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
    TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
    ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
    TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
    AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
    CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
    GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
    TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
    GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
    GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
    TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
    TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGGGCCAT CAGTAAATAA AGCCAAAAGT
    TGTGCCACTG AAGAGCGATA CAAAGGAACA GAGCGCGAAC GGCTTCTAAA TTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27327
    Clone ID Related Accession (Same CDS sequence) NM_000271.5 , NM_000271.4
    Accession Version NM_000271.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3837bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-17
    Organism Homo sapiens(human)
    Product Niemann-Pick C1 protein precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR159421.1, AF002020.1, AB209048.1 and BC063302.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2009 this sequence version replaced NM_000271.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF002020.1, SRR1803612.141336.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
    GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
    CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
    ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
    TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
    CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
    CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
    CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
    ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
    ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
    GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
    GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
    GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
    CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
    CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
    ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
    GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
    CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
    ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
    CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
    AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
    GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
    TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
    TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
    AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
    GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
    TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
    ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
    GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
    CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
    TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
    CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
    TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
    AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
    CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
    ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
    TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
    TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
    ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
    TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
    AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
    CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
    GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
    TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
    GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
    GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
    TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
    TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGGGCCAT CAGTAAATAA AGCCAAAAGT
    TGTGCCACTG AAGAGCGATA CAAAGGAACA GAGCGCGAAC GGCTTCTAAA TTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000262.2
    CDS275..4111
    Translation

    Target ORF information:

    RefSeq Version NM_000271.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000271.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
    GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
    CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
    ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
    TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
    CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
    CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
    CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
    ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
    ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
    GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
    GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
    GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
    CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
    CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
    ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
    GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
    CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
    ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
    CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
    AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
    GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
    TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
    TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
    AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
    GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
    TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
    ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
    GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
    CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
    TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
    CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
    TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
    AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
    CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
    ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
    TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
    TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
    ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
    TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
    AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
    CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
    GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
    TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
    GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
    GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
    TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
    TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGGGCCAT CAGTAAATAA AGCCAAAAGT
    TGTGCCACTG AAGAGCGATA CAAAGGAACA GAGCGCGAAC GGCTTCTAAA TTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51216
    Clone ID Related Accession (Same CDS sequence) XM_005258277.1
    Accession Version XM_005258277.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product NPC intracellular cholesterol transporter 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
    GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
    TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
    TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
    TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
    CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
    CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
    CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
    CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
    CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
    GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
    TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
    AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
    TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
    ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
    GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
    ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
    GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
    AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
    GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
    CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
    ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
    CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
    GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
    GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
    GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
    GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
    TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
    GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
    CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
    CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
    GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
    TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
    GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
    GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
    CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
    TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
    AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
    GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
    AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
    TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
    GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
    GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
    CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
    TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
    GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
    CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
    TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
    CTCCCTGTCT TACTCAGTTA CATAGCAAAG CTTCTGGAAA GAACTATCTA CTTGTGCATC
    TCACAGCACA TTCATTATGC AACCCAGATG GCTCCACTAC AGTGTCTCAT GACCGCCTCA
    GACAGATGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005258334.1
    CDS164..4075
    Translation

    Target ORF information:

    RefSeq Version XM_005258277.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005258277.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
    GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
    TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
    TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
    TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
    CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
    CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
    CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
    CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
    CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
    GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
    TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
    AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
    TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
    ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
    GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
    ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
    GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
    AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
    GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
    CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
    ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
    CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
    GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
    GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
    GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
    GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
    TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
    GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
    CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
    CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
    GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
    TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
    GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
    GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
    CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
    TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
    AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
    GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
    AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
    TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
    GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
    GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
    CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
    TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
    GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
    CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
    TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
    CTCCCTGTCT TACTCAGTTA CATAGCAAAG CTTCTGGAAA GAACTATCTA CTTGTGCATC
    TCACAGCACA TTCATTATGC AACCCAGATG GCTCCACTAC AGTGTCTCAT GACCGCCTCA
    GACAGATGTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51217
    Clone ID Related Accession (Same CDS sequence) XM_005258278.5 , XM_005258278.4
    Accession Version XM_005258278.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product NPC intracellular cholesterol transporter 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005258278.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
    GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
    TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
    TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
    TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
    CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
    CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
    CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
    CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
    CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
    GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
    TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
    AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
    TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
    ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
    GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
    ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
    GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
    AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
    GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
    CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
    ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
    CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
    GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
    GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
    GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
    GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
    TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
    GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
    CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
    CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
    GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
    TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
    GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
    GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
    CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
    TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
    AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
    GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
    AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
    TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
    GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
    GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
    CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
    TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
    GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
    CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
    TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
    CTCCCTGTCT TACTCAGTTA CATAGGGCCA TCAGTAAATA AAGCCAAAAG TTGTGCCACT
    GAAGAGCGAT ACAAAGGAAC AGAGCGCGAA CGGCTTCTAA ATTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005258335.1
    CDS164..4051
    Translation

    Target ORF information:

    RefSeq Version XM_005258278.5
    Organism Homo sapiens(human)
    Definition Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005258278.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
    GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
    TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
    TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
    TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
    CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
    CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
    CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
    CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
    CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
    GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
    TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
    AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
    TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
    ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
    GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
    ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
    GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
    AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
    GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
    CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
    ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
    CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
    GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
    GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
    GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
    GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
    TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
    GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
    CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
    CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
    GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
    TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
    GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
    GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
    CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
    TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
    AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
    GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
    AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
    TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
    GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
    GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
    CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
    TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
    GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
    CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
    TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
    CTCCCTGTCT TACTCAGTTA CATAGGGCCA TCAGTAAATA AAGCCAAAAG TTGTGCCACT
    GAAGAGCGAT ACAAAGGAAC AGAGCGCGAA CGGCTTCTAA ATTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51217
    Clone ID Related Accession (Same CDS sequence) XM_005258278.5 , XM_005258278.4
    Accession Version XM_005258278.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Niemann-Pick C1 protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005258278.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
    GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
    TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
    TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
    TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
    CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
    CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
    CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
    CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
    CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
    GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
    TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
    AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
    TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
    ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
    GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
    ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
    GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
    AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
    GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
    CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
    ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
    CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
    GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
    GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
    GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
    GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
    TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
    GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
    CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
    CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
    GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
    TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
    GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
    GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
    CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
    TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
    AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
    GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
    AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
    TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
    GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
    GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
    CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
    TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
    GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
    CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
    TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
    CTCCCTGTCT TACTCAGTTA CATAGGGCCA TCAGTAAATA AAGCCAAAAG TTGTGCCACT
    GAAGAGCGAT ACAAAGGAAC AGAGCGCGAA CGGCTTCTAA ATTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005258335.1
    CDS164..4051
    Translation

    Target ORF information:

    RefSeq Version XM_005258278.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005258278.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
    GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
    TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
    TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
    TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
    CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
    CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
    CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
    CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
    CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
    GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
    TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
    AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
    TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
    ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
    GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
    ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
    GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
    AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
    GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
    CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
    ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
    CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
    GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
    GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
    GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
    GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
    TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
    GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
    CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
    CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
    GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
    TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
    GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
    GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
    CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
    TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
    AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
    GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
    AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
    TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
    GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
    GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
    CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
    TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
    GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
    CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
    TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
    CTCCCTGTCT TACTCAGTTA CATAGGGCCA TCAGTAAATA AAGCCAAAAG TTGTGCCACT
    GAAGAGCGAT ACAAAGGAAC AGAGCGCGAA CGGCTTCTAA ATTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51218
    Clone ID Related Accession (Same CDS sequence) XM_005258279.2
    Accession Version XM_005258279.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product NPC intracellular cholesterol transporter 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005258279.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
    GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
    CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
    ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
    TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
    CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
    CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
    CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
    ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
    ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
    GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
    GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
    GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
    CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
    CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
    ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
    GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
    CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
    ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
    CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
    AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
    GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
    TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
    TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
    AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
    GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
    TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
    ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
    GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
    CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
    TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
    CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
    TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
    AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
    CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
    ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
    TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
    TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
    ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
    TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
    AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
    CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
    GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
    TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
    GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
    GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
    TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
    TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGCAAAGC TTCTGGAAAG AACTATCTAC
    TTGTGCATCT CACAGCACAT TCATTATGCA ACCCAGATGG CTCCACTACA GTGTCTCATG
    ACCGCCTCAG ACAGATGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005258336.1
    CDS164..4024
    Translation

    Target ORF information:

    RefSeq Version XM_005258279.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005258279.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
    GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
    CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
    ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
    TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
    CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
    CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
    CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
    ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
    ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
    GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
    GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
    GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
    CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
    CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
    ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
    GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
    CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
    ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
    CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
    AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
    GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
    TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
    TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
    AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
    GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
    TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
    ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
    GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
    CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
    TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
    CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
    TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
    AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
    CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
    ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
    TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
    TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
    ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
    TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
    AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
    CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
    GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
    TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
    GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
    GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
    TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
    TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGCAAAGC TTCTGGAAAG AACTATCTAC
    TTGTGCATCT CACAGCACAT TCATTATGCA ACCCAGATGG CTCCACTACA GTGTCTCATG
    ACCGCCTCAG ACAGATGTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51219
    Clone ID Related Accession (Same CDS sequence) XM_006722479.3
    Accession Version XM_006722479.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product NPC intracellular cholesterol transporter 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722479.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
    GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
    TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
    TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
    TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
    CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
    CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
    CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
    CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
    CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
    GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
    TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
    AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
    TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
    ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
    GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
    ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
    GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
    AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
    GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
    CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
    ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
    CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
    GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
    GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
    GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
    GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
    TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
    GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
    CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
    CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
    GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
    TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
    GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
    GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
    CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
    TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
    AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
    GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
    AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
    TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
    GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
    GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
    CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
    TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
    GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
    CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
    TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
    CTCCCTGTCT TACTCAGTTA CATAGGCCCA TTCAATTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006722542.1
    CDS164..3985
    Translation

    Target ORF information:

    RefSeq Version XM_006722479.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006722479.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
    TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
    AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
    GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
    CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
    TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
    TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
    GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
    CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
    GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
    GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
    AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
    CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
    ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
    CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
    GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
    GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
    TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
    TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
    TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
    CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
    CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
    CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
    CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
    CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
    GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
    TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
    AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
    TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
    ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
    GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
    ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
    GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
    AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
    GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
    CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
    ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
    CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
    GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
    GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
    GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
    GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
    TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
    GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
    CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
    CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
    GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
    TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
    GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
    GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
    CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
    TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
    AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
    GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
    AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
    TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
    GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
    GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
    CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
    TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
    GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
    CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
    TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
    CTCCCTGTCT TACTCAGTTA CATAGGCCCA TTCAATTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59314
    Clone ID Related Accession (Same CDS sequence) XM_011526015.1
    Accession Version XM_011526015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product Niemann-Pick C1 protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000018.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTACAATG CCTGCCGGGA TGTGGAGGCC CCCTCAAGTA ATGACAAGGC CCTGGGACTC 
    CTGTGTGGGA AGGACGCTGA CGCCTGTAAT GCCACCAACT GGATTGAATA CATGTTCAAT
    AAGGACAATG GACAGGCACC TTTTACCATC ACTCCTGTGT TTTCAGATTT TCCAGTCCAT
    GGGATGGAGC CCATGAACAA TGCCACCAAA GGCTGTGACG AGTCTGTGGA TGAGGTCACA
    GCACCATGTA GCTGCCAAGA CTGCTCTATT GTCTGTGGCC CCAAGCCCCA GCCCCCACCT
    CCTCCTGCTC CCTGGACGAT CCTTGGCTTG GACGCCATGT ATGTCATCAT GTGGATCACC
    TACATGGCGT TTTTGCTTGT GTTTTTTGGA GCATTTTTTG CAGTGTGGTG CTACAGAAAA
    CGGTATTTTG TCTCCGAGTA CACTCCCATC GATAGCAATA TAGCTTTTTC TGTTAATGCA
    AGTGACAAAG GAACGGCTTG GCTCTTAACC TCCACCTTCC CTTCCTCTCC CGTTCTTCCA
    GGAGAGGCGT CCTGCTGTGA CCCTGTCAGC GCAGCATTTG AGGGCTGCTT GAGGCGGCTG
    TTCACACGCT GGGGGTCTTT CTGCGTCCGA AACCCTGGCT GTGTCATTTT CTTCTCGCTG
    GTCTTCATTA CTGCGTGTTC GTCAGGCCTG GTGTTTGTCC GGGTCACAAC CAATCCAGTT
    GACCTCTGGT CAGCCCCCAG CAGCCAGGCT CGCCTGGAAA AAGAGTACTT TGACCAGCAC
    TTTGGGCCTT TCTTCCGGAC GGAGCAGCTC ATCATCCGGG CCCCTCTCAC TGACAAACAC
    ATTTACCAGC CATACCCTTC GGGAGCTGAT GTACCCTTTG GACCTCCGCT TGACATACAG
    ATACTGCACC AGGTTCTTGA CTTACAAATA GCCATCGAAA ACATTACTGC CTCTTATGAC
    AATGAGACTG TGACACTTCA AGACATCTGC TTGGCCCCTC TTTCACCGTA TAACACGAAC
    TGCACCATTT TGAGTGTGTT AAATTACTTC CAGAACAGCC ATTCCGTGCT GGACCACAAG
    AAAGGGGACG ACTTCTTTGT GTATGCCGAT TACCACACGC ACTTTCTGTA CTGCGTACGG
    GCTCCTGCCT CTCTGAATGA TACAAGTTTG CTCCATGACC CTTGTCTGGG TACGTTTGGT
    GGACCAGTGT TCCCGTGGCT TGTGTTGGGA GGCTATGATG ATCAAAACTA CAATAACGCC
    ACTGCCCTTG TGATTACCTT CCCTGTCAAT AATTACTATA ATGATACAGA GAAGCTCCAG
    AGGGCCCAGG CCTGGGAAAA AGAGTTTATT AATTTTGTGA AAAACTACAA GAATCCCAAT
    CTGACCATTT CCTTCACTGC TGAACGAAGT ATTGAAGATG AACTAAATCG TGAAAGTGAC
    AGTGATGTCT TCACCGTTGT AATTAGCTAT GCCATCATGT TTCTATATAT TTCCCTAGCC
    TTGGGGCACA TGAAAAGCTG TCGCAGGCTT CTGGTGGATT CGAAGGTCTC ACTAGGCATC
    GCGGGCATCT TGATCGTGCT GAGCTCGGTG GCTTGCTCCT TGGGTGTCTT CAGCTACATT
    GGGTTGCCCT TGACCCTCAT TGTGATTGAA GTCATCCCGT TCCTGGTGCT GGCTGTTGGA
    GTGGACAACA TCTTCATTCT GGTGCAGGCC TACCAGAGAG ATGAACGTCT TCAAGGGGAA
    ACCCTGGATC AGCAGCTGGG CAGGGTCCTA GGAGAAGTGG CTCCCAGTAT GTTCCTGTCA
    TCCTTTTCTG AGACTGTAGC ATTTTTCTTA GGAGCATTGT CCGTGATGCC AGCCGTGCAC
    ACCTTCTCTC TCTTTGCGGG ATTGGCAGTC TTCATTGACT TTCTTCTGCA GATTACCTGT
    TTCGTGAGTC TCTTGGGGTT AGACATTAAA CGTCAAGAGA AAAATCGGCT AGACATCTTT
    TGCTGTGTCA GAGGTGCTGA AGATGGAACA AGCGTCCAGG CCTCAGAGAG CTGTTTGTTT
    CGCTTCTTCA AAAACTCCTA TTCTCCACTT CTGCTAAAGG ACTGGATGAG ACCAATTGTG
    ATAGCAATAT TTGTGGGTGT TCTGTCATTC AGCATCGCAG TCCTGAACAA AGTAGATATT
    GGATTGGATC AGTCTCTTTC GATGCCAGAT GACTCCTACA TGGTGGATTA TTTCAAATCC
    ATCAGTCAGT ACCTGCATGC GGGTCCGCCT GTGTACTTTG TCCTGGAGGA AGGGCACGAC
    TACACTTCTT CCAAGGGGCA GAACATGGTG TGCGGCGGCA TGGGCTGCAA CAATGATTCC
    CTGGTGCAGC AGATATTTAA CGCGGCGCAG CTGGACAACT ATACCCGAAT AGGCTTCGCC
    CCCTCGTCCT GGATCGACGA TTATTTCGAC TGGGTGAAGC CACAGTCGTC TTGCTGTCGA
    GTGGACAATA TCACTGACCA GTTCTGCAAT GCTTCAGTGG TTGACCCTGC CTGCGTTCGC
    TGCAGGCCTC TGACTCCGGA AGGCAAACAG AGGCCTCAGG GGGGAGACTT CATGAGATTC
    CTGCCCATGT TCCTTTCGGA TAACCCTAAC CCCAAGTGTG GCAAAGGGGG ACATGCTGCC
    TATAGTTCTG CAGTTAACAT CCTCCTTGGC CATGGCACCA GGGTCGGAGC CACGTACTTC
    ATGACCTACC ACACCGTGCT GCAGACCTCT GCTGACTTTA TTGACGCTCT GAAGAAAGCC
    CGACTTATAG CCAGTAATGT CACCGAAACC ATGGGCATTA ACGGCAGTGC CTACCGAGTA
    TTTCCTTACA GTGTGTTTTA TGTCTTCTAC GAACAGTACC TGACCATCAT TGACGACACT
    ATCTTCAACC TCGGTGTGTC CCTGGGCGCG ATATTTCTGG TGACCATGGT CCTCCTGGGC
    TGTGAGCTCT GGTCTGCAGT CATCATGTGT GCCACCATCG CCATGGTCTT GGTCAACATG
    TTTGGAGTTA TGTGGCTCTG GGGCATCAGT CTGAACGCTG TATCCTTGGT CAACCTGGTG
    ATGAGCTGTG GCATCTCCGT GGAGTTCTGC AGCCACATAA CCAGAGCGTT CACGGTGAGC
    ATGAAAGGCA GCCGCGTGGA GCGCGCGGAA GAGGCACTTG CCCACATGGG CAGCTCCGTG
    TTCAGTGGAA TCACACTTAC AAAATTTGGA GGGATTGTGG TGTTGGCTTT TGCCAAATCT
    CAAATTTTCC AGATATTCTA CTTCAGGATG TATTTGGCCA TGGTCTTACT GGGAGCCACT
    CACGGATTAA TATTTCTCCC TGTCTTACTC AGTTACATAG CAAAGCTTCT GGAAAGAACT
    ATCTACTTGT GCATCTCACA GCACATTCAT TATGCAACCC AGATGGCTCC ACTACAGTGT
    CTCATGACCG CCTCAGACAG ATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011524317.1
    CDS877..4323
    Translation

    Target ORF information:

    RefSeq Version XM_011526015.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NPC intracellular cholesterol transporter 1 (NPC1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011526015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTACAATG CCTGCCGGGA TGTGGAGGCC CCCTCAAGTA ATGACAAGGC CCTGGGACTC 
    CTGTGTGGGA AGGACGCTGA CGCCTGTAAT GCCACCAACT GGATTGAATA CATGTTCAAT
    AAGGACAATG GACAGGCACC TTTTACCATC ACTCCTGTGT TTTCAGATTT TCCAGTCCAT
    GGGATGGAGC CCATGAACAA TGCCACCAAA GGCTGTGACG AGTCTGTGGA TGAGGTCACA
    GCACCATGTA GCTGCCAAGA CTGCTCTATT GTCTGTGGCC CCAAGCCCCA GCCCCCACCT
    CCTCCTGCTC CCTGGACGAT CCTTGGCTTG GACGCCATGT ATGTCATCAT GTGGATCACC
    TACATGGCGT TTTTGCTTGT GTTTTTTGGA GCATTTTTTG CAGTGTGGTG CTACAGAAAA
    CGGTATTTTG TCTCCGAGTA CACTCCCATC GATAGCAATA TAGCTTTTTC TGTTAATGCA
    AGTGACAAAG GAACGGCTTG GCTCTTAACC TCCACCTTCC CTTCCTCTCC CGTTCTTCCA
    GGAGAGGCGT CCTGCTGTGA CCCTGTCAGC GCAGCATTTG AGGGCTGCTT GAGGCGGCTG
    TTCACACGCT GGGGGTCTTT CTGCGTCCGA AACCCTGGCT GTGTCATTTT CTTCTCGCTG
    GTCTTCATTA CTGCGTGTTC GTCAGGCCTG GTGTTTGTCC GGGTCACAAC CAATCCAGTT
    GACCTCTGGT CAGCCCCCAG CAGCCAGGCT CGCCTGGAAA AAGAGTACTT TGACCAGCAC
    TTTGGGCCTT TCTTCCGGAC GGAGCAGCTC ATCATCCGGG CCCCTCTCAC TGACAAACAC
    ATTTACCAGC CATACCCTTC GGGAGCTGAT GTACCCTTTG GACCTCCGCT TGACATACAG
    ATACTGCACC AGGTTCTTGA CTTACAAATA GCCATCGAAA ACATTACTGC CTCTTATGAC
    AATGAGACTG TGACACTTCA AGACATCTGC TTGGCCCCTC TTTCACCGTA TAACACGAAC
    TGCACCATTT TGAGTGTGTT AAATTACTTC CAGAACAGCC ATTCCGTGCT GGACCACAAG
    AAAGGGGACG ACTTCTTTGT GTATGCCGAT TACCACACGC ACTTTCTGTA CTGCGTACGG
    GCTCCTGCCT CTCTGAATGA TACAAGTTTG CTCCATGACC CTTGTCTGGG TACGTTTGGT
    GGACCAGTGT TCCCGTGGCT TGTGTTGGGA GGCTATGATG ATCAAAACTA CAATAACGCC
    ACTGCCCTTG TGATTACCTT CCCTGTCAAT AATTACTATA ATGATACAGA GAAGCTCCAG
    AGGGCCCAGG CCTGGGAAAA AGAGTTTATT AATTTTGTGA AAAACTACAA GAATCCCAAT
    CTGACCATTT CCTTCACTGC TGAACGAAGT ATTGAAGATG AACTAAATCG TGAAAGTGAC
    AGTGATGTCT TCACCGTTGT AATTAGCTAT GCCATCATGT TTCTATATAT TTCCCTAGCC
    TTGGGGCACA TGAAAAGCTG TCGCAGGCTT CTGGTGGATT CGAAGGTCTC ACTAGGCATC
    GCGGGCATCT TGATCGTGCT GAGCTCGGTG GCTTGCTCCT TGGGTGTCTT CAGCTACATT
    GGGTTGCCCT TGACCCTCAT TGTGATTGAA GTCATCCCGT TCCTGGTGCT GGCTGTTGGA
    GTGGACAACA TCTTCATTCT GGTGCAGGCC TACCAGAGAG ATGAACGTCT TCAAGGGGAA
    ACCCTGGATC AGCAGCTGGG CAGGGTCCTA GGAGAAGTGG CTCCCAGTAT GTTCCTGTCA
    TCCTTTTCTG AGACTGTAGC ATTTTTCTTA GGAGCATTGT CCGTGATGCC AGCCGTGCAC
    ACCTTCTCTC TCTTTGCGGG ATTGGCAGTC TTCATTGACT TTCTTCTGCA GATTACCTGT
    TTCGTGAGTC TCTTGGGGTT AGACATTAAA CGTCAAGAGA AAAATCGGCT AGACATCTTT
    TGCTGTGTCA GAGGTGCTGA AGATGGAACA AGCGTCCAGG CCTCAGAGAG CTGTTTGTTT
    CGCTTCTTCA AAAACTCCTA TTCTCCACTT CTGCTAAAGG ACTGGATGAG ACCAATTGTG
    ATAGCAATAT TTGTGGGTGT TCTGTCATTC AGCATCGCAG TCCTGAACAA AGTAGATATT
    GGATTGGATC AGTCTCTTTC GATGCCAGAT GACTCCTACA TGGTGGATTA TTTCAAATCC
    ATCAGTCAGT ACCTGCATGC GGGTCCGCCT GTGTACTTTG TCCTGGAGGA AGGGCACGAC
    TACACTTCTT CCAAGGGGCA GAACATGGTG TGCGGCGGCA TGGGCTGCAA CAATGATTCC
    CTGGTGCAGC AGATATTTAA CGCGGCGCAG CTGGACAACT ATACCCGAAT AGGCTTCGCC
    CCCTCGTCCT GGATCGACGA TTATTTCGAC TGGGTGAAGC CACAGTCGTC TTGCTGTCGA
    GTGGACAATA TCACTGACCA GTTCTGCAAT GCTTCAGTGG TTGACCCTGC CTGCGTTCGC
    TGCAGGCCTC TGACTCCGGA AGGCAAACAG AGGCCTCAGG GGGGAGACTT CATGAGATTC
    CTGCCCATGT TCCTTTCGGA TAACCCTAAC CCCAAGTGTG GCAAAGGGGG ACATGCTGCC
    TATAGTTCTG CAGTTAACAT CCTCCTTGGC CATGGCACCA GGGTCGGAGC CACGTACTTC
    ATGACCTACC ACACCGTGCT GCAGACCTCT GCTGACTTTA TTGACGCTCT GAAGAAAGCC
    CGACTTATAG CCAGTAATGT CACCGAAACC ATGGGCATTA ACGGCAGTGC CTACCGAGTA
    TTTCCTTACA GTGTGTTTTA TGTCTTCTAC GAACAGTACC TGACCATCAT TGACGACACT
    ATCTTCAACC TCGGTGTGTC CCTGGGCGCG ATATTTCTGG TGACCATGGT CCTCCTGGGC
    TGTGAGCTCT GGTCTGCAGT CATCATGTGT GCCACCATCG CCATGGTCTT GGTCAACATG
    TTTGGAGTTA TGTGGCTCTG GGGCATCAGT CTGAACGCTG TATCCTTGGT CAACCTGGTG
    ATGAGCTGTG GCATCTCCGT GGAGTTCTGC AGCCACATAA CCAGAGCGTT CACGGTGAGC
    ATGAAAGGCA GCCGCGTGGA GCGCGCGGAA GAGGCACTTG CCCACATGGG CAGCTCCGTG
    TTCAGTGGAA TCACACTTAC AAAATTTGGA GGGATTGTGG TGTTGGCTTT TGCCAAATCT
    CAAATTTTCC AGATATTCTA CTTCAGGATG TATTTGGCCA TGGTCTTACT GGGAGCCACT
    CACGGATTAA TATTTCTCCC TGTCTTACTC AGTTACATAG CAAAGCTTCT GGAAAGAACT
    ATCTACTTGT GCATCTCACA GCACATTCAT TATGCAACCC AGATGGCTCC ACTACAGTGT
    CTCATGACCG CCTCAGACAG ATGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.