NF1 cDNA ORF clone, Homo sapiens(human)

The following NF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu26980 NM_001128147.3
Latest version!
Homo sapiens neurofibromin 1 (NF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu86895 XM_017024690.1
Latest version!
Homo sapiens neurofibromin 1 (NF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu23641 NM_001042492.3
Latest version!
Homo sapiens neurofibromin 1 (NF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu24060 NM_000267.3
Latest version!
Homo sapiens neurofibromin 1 (NF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu50427 XM_006721922.2
Latest version!
Homo sapiens neurofibromin 1 (NF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu50428 XM_005257983.2
Latest version!
Homo sapiens neurofibromin 1 (NF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu50430 XM_006721924.2
Latest version!
Homo sapiens neurofibromin 1 (NF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu50431 XM_006721925.2
Latest version!
Homo sapiens neurofibromin 1 (NF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu50432 XM_005257984.2
Latest version!
Homo sapiens neurofibromin 1 (NF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu50433 XM_006721926.3
Latest version!
Homo sapiens neurofibromin 1 (NF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu59245 XM_011524852.2
Latest version!
Homo sapiens neurofibromin 1 (NF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu59246 XM_011524857.2
Latest version!
Homo sapiens neurofibromin 1 (NF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu86894 XM_017024689.1
Latest version!
Homo sapiens neurofibromin 1 (NF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OHu23641 NM_001042492.2 Homo sapiens neurofibromin 1 (NF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OHu26980 NM_001128147.2 Homo sapiens neurofibromin 1 (NF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu26980
    Clone ID Related Accession (Same CDS sequence) NM_001128147.2 , NM_001128147.3
    Accession Version NM_001128147.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product neurofibromin isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D12625.1, M82814.1 and D42072.1. On Dec 3, 2009 this sequence version replaced NM_001128147.1. Transcript Variant: This variant (3) lacks multiple 3' exons and has an alternate 3' end, as compared to variant 1. The resulting isoform (3) has a much shorter and different C-terminus, and lacks ras-GTPase activating domain and SEC14 domain, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D42072.1, AK289936.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## undergoes RNA editing :: PMID: 8602361, 11727199 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GGTATATGTA CTTTTATTTT
    TTAAATTCAA CTTTTAAATT TTATTTTGTA TTTTTGTCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121619.1
    CDS384..2165
    Translation

    Target ORF information:

    RefSeq Version NM_001128147.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001128147.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GGTATATGTA CTTTTATTTT
    TTAAATTCAA CTTTTAAATT TTATTTTGTA TTTTTGTCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26980
    Clone ID Related Accession (Same CDS sequence) NM_001128147.2 , NM_001128147.3
    Accession Version NM_001128147.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product neurofibromin isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D12625.1, M82814.1 and D42072.1. On Jun 2, 2019 this sequence version replaced NM_001128147.2. Transcript Variant: This variant (3) lacks multiple 3' exons and has an alternate 3' end, as compared to variant 1. The resulting isoform (3) has a much shorter and different C-terminus, and lacks ras-GTPase activating domain and SEC14 domain, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D42072.1, AK289936.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## undergoes RNA editing :: PMID: 8602361, 11727199 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GGTATATGTA CTTTTATTTT
    TTAAATTCAA CTTTTAAATT TTATTTTGTA TTTTTGTCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121619.1
    CDS334..2115
    Translation

    Target ORF information:

    RefSeq Version NM_001128147.3
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001128147.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GGTATATGTA CTTTTATTTT
    TTAAATTCAA CTTTTAAATT TTATTTTGTA TTTTTGTCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86895
    Clone ID Related Accession (Same CDS sequence) XM_017024690.1
    Accession Version XM_017024690.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurofibromin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    AGTCATCGCA GCCATTTGCT TTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880179.1
    CDS334..4500
    Translation

    Target ORF information:

    RefSeq Version XM_017024690.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024690.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    AGTCATCGCA GCCATTTGCT TTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23641
    Clone ID Related Accession (Same CDS sequence) NM_001042492.3 , NM_001042492.2
    Accession Version NM_001042492.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product neurofibromin isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D12625.1, M89914.1, BX391799.2, BP271334.1, AC135724.9 and AK026658.1. On Aug 31, 2019 this sequence version replaced NM_001042492.2. Transcript Variant: This variant (1), with an additional in-frame coding exon, represents the longest transcript and encodes the longest isoform (1). Studies suggest preferential C->U RNA editing of transcripts containing this exon. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M89914.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein undergoes RNA editing :: PMID: 8602361, 11727199 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
    CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
    GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
    CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
    GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
    TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
    TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
    GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
    CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
    GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
    AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
    ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
    GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
    ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
    TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
    GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
    ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
    GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
    GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
    GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
    GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
    ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
    GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
    GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
    CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
    GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
    CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
    CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
    GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
    TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
    CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
    CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
    TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
    GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
    CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
    GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
    CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
    CTTCGAAGTG TGTGCCACTG TTTATACCAG GCAACTTGCC ACTCCCTACT GAATAAAGCT
    ACAGTAAAAG AAAAAAAGGA AAACAAAAAA TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC
    AGCATCGGTG CAGTAGGAAG TGCCATGTTC CTCAGATTTA TCAATCCTGC CATTGTCTCA
    CCGTATGAAG CAGGGATTTT AGATAAAAAG CCACCACCTA GAATCGAAAG GGGCTTGAAG
    TTAATGTCAA AGATACTTCA GAGTATTGCC AATCATGTTC TCTTCACAAA AGAAGAACAT
    ATGCGGCCTT TCAATGATTT TGTGAAAAGC AACTTTGATG CAGCACGCAG GTTTTTCCTT
    GATATAGCAT CTGATTGTCC TACAAGTGAT GCAGTAAATC ATAGTCTTTC CTTCATAAGT
    GACGGCAATG TGCTTGCTTT ACATCGTCTA CTCTGGAACA ATCAGGAGAA AATTGGGCAG
    TATCTTTCCA GCAACAGGGA TCATAAAGCT GTTGGAAGAC GACCTTTTGA TAAGATGGCA
    ACACTTCTTG CATACCTGGG TCCTCCAGAG CACAAACCTG TGGCAGATAC ACACTGGTCC
    AGCCTTAACC TTACCAGTTC AAAGTTTGAG GAATTTATGA CTAGGCATCA GGTACATGAA
    AAAGAAGAAT TCAAGGCTTT GAAAACGTTA AGTATTTTCT ACCAAGCTGG GACTTCCAAA
    GCTGGGAATC CTATTTTTTA TTATGTTGCA CGGAGGTTCA AAACTGGTCA AATCAATGGT
    GATTTGCTGA TATACCATGT CTTACTGACT TTAAAGCCAT ATTATGCAAA GCCATATGAA
    ATTGTAGTGG ACCTTACCCA TACCGGGCCT AGCAATCGCT TTAAAACAGA CTTTCTCTCT
    AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT TACGACAACG TCTCCGCAGT CTATATCTAT
    AACTGTAACT CCTGGGTCAG GGAGTACACC AAGTATCATG AGCGGCTGCT GACTGGCCTC
    AAAGGTAGCA AAAGGCTTGT TTTCATAGAC TGTCCTGGGA AACTGGCTGA GCACATAGAG
    CATGAACAAC AGAAACTACC TGCTGCCACC TTGGCTTTAG AAGAGGACCT GAAGGTATTC
    CACAATGCTC TCAAGCTAGC TCACAAAGAC ACCAAAGTTT CTATTAAAGT TGGTTCTACT
    GCTGTCCAAG TAACTTCAGC AGAGCGAACA AAAGTCCTAG GGCAATCAGT CTTTCTAAAT
    GACATTTATT ATGCTTCGGA AATTGAAGAA ATCTGCCTAG TAGATGAGAA CCAGTTCACC
    TTAACCATTG CAAACCAGGG CACGCCGCTC ACCTTCATGC ACCAGGAGTG TGAAGCCATT
    GTCCAGTCTA TCATTCATAT CCGGACCCGC TGGGAACTGT CACAGCCCGA CTCTATCCCC
    CAACACACCA AGATTCGGCC AAAAGATGTC CCTGGGACAC TGCTCAATAT CGCATTACTT
    AATTTAGGCA GTTCTGACCC GAGTTTACGG TCAGCTGCCT ATAATCTTCT GTGTGCCTTA
    ACTTGTACCT TTAATTTAAA AATCGAGGGC CAGTTACTAG AGACATCAGG TTTATGTATC
    CCTGCCAACA ACACCCTCTT TATTGTCTCT ATTAGTAAGA CACTGGCAGC CAATGAGCCA
    CACCTCACGT TAGAATTTTT GGAAGAGTGT ATTTCTGGAT TTAGCAAATC TAGTATTGAA
    TTGAAACACC TTTGTTTGGA ATACATGACT CCATGGCTGT CAAATCTAGT TCGTTTTTGC
    AAGCATAATG ATGATGCCAA ACGACAAAGA GTTACTGCTA TTCTTGACAA GCTGATAACA
    ATGACCATCA ATGAAAAACA GATGTACCCA TCTATTCAAG CAAAAATATG GGGAAGCCTT
    GGGCAGATTA CAGATCTGCT TGATGTTGTA CTAGACAGTT TCATCAAAAC CAGTGCAACA
    GGTGGCTTGG GATCAATAAA AGCTGAGGTG ATGGCAGATA CTGCTGTAGC TTTGGCTTCT
    GGAAATGTGA AATTGGTTTC AAGCAAGGTT ATTGGAAGGA TGTGCAAAAT AATTGACAAG
    ACATGCTTAT CTCCAACTCC TACTTTAGAA CAACATCTTA TGTGGGATGA TATTGCTATT
    TTAGCACGCT ACATGCTGAT GCTGTCCTTC AACAATTCCC TTGATGTGGC AGCTCATCTT
    CCCTACCTCT TCCACGTTGT TACTTTCTTA GTAGCCACAG GTCCGCTCTC CCTTAGAGCT
    TCCACACATG GACTGGTCAT TAATATCATT CACTCTCTGT GTACTTGTTC ACAGCTTCAT
    TTTAGTGAAG AGACCAAGCA AGTTTTGAGA CTCAGTCTGA CAGAGTTCTC ATTACCCAAA
    TTTTACTTGC TGTTTGGCAT TAGCAAAGTC AAGTCAGCTG CTGTCATTGC CTTCCGTTCC
    AGTTACCGGG ACAGGTCATT CTCTCCTGGC TCCTATGAGA GAGAGACTTT TGCTTTGACA
    TCCTTGGAAA CAGTCACAGA AGCTTTGTTG GAGATCATGG AGGCATGCAT GAGAGATATT
    CCAACGTGCA AGTGGCTGGA CCAGTGGACA GAACTAGCTC AAAGATTTGC ATTCCAATAT
    AATCCATCCC TGCAACCAAG AGCTCTTGTT GTCTTTGGGT GTATTAGCAA ACGAGTGTCT
    CATGGGCAGA TAAAGCAGAT AATCCGTATT CTTAGCAAGG CACTTGAGAG TTGCTTAAAA
    GGACCTGACA CTTACAACAG TCAAGTTCTG ATAGAAGCTA CAGTAATAGC ACTAACCAAA
    TTACAGCCAC TTCTTAATAA GGACTCGCCT CTGCACAAAG CCCTCTTTTG GGTAGCTGTG
    GCTGTGCTGC AGCTTGATGA GGTCAACTTG TATTCAGCAG GTACCGCACT TCTTGAACAA
    AACCTGCATA CTTTAGATAG TCTCCGTATA TTCAATGACA AGAGTCCAGA GGAAGTATTT
    ATGGCAATCC GGAATCCTCT GGAGTGGCAC TGCAAGCAAA TGGATCATTT TGTTGGACTC
    AATTTCAACT CTAACTTTAA CTTTGCATTG GTTGGACACC TTTTAAAAGG GTACAGGCAT
    CCTTCACCTG CTATTGTTGC AAGAACAGTC AGAATTTTAC ATACACTACT AACTCTGGTT
    AACAAACACA GAAATTGTGA CAAATTTGAA GTGAATACAC AGAGCGTGGC CTACTTAGCA
    GCTTTACTTA CAGTGTCTGA AGAAGTTCGA AGTCGCTGCA GCCTAAAACA TAGAAAGTCA
    CTTCTTCTTA CTGATATTTC AATGGAAAAT GTTCCTATGG ATACATATCC CATTCATCAT
    GGTGACCCTT CCTATAGGAC ACTAAAGGAG ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT
    GAAGGATACC TTGCAGCCAC CTATCCAACT GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA
    TCCATGAGCC TGGACATGGG GCAACCTTCT CAGGCCAACA CTAAGAAGTT GCTTGGAACA
    AGGAAAAGTT TTGATCACTT GATATCAGAC ACAAAGGCTC CTAAAAGGCA AGAAATGGAA
    TCAGGGATCA CAACACCCCC CAAAATGAGG AGAGTAGCAG AAACTGATTA TGAAATGGAA
    ACTCAGAGGA TTTCCTCATC ACAACAGCAC CCACATTTAC GTAAAGTTTC AGTGTCTGAA
    TCAAATGTTC TCTTGGATGA AGAAGTACTT ACTGATCCGA AGATCCAGGC GCTGCTTCTT
    ACTGTTCTAG CTACACTGGT AAAATATACC ACAGATGAGT TTGATCAACG AATTCTTTAT
    GAATACTTAG CAGAGGCCAG TGTTGTGTTT CCCAAAGTCT TTCCTGTTGT GCATAATTTG
    TTGGACTCTA AGATCAACAC CCTGTTATCA TTGTGCCAAG ATCCAAATTT GTTAAATCCA
    ATCCATGGAA TTGTGCAGAG TGTGGTGTAC CATGAAGAAT CCCCACCACA ATACCAAACA
    TCTTACCTGC AAAGTTTTGG TTTTAATGGC TTGTGGCGGT TTGCAGGACC GTTTTCAAAG
    CAAACACAAA TTCCAGACTA TGCTGAGCTT ATTGTTAAGT TTCTTGATGC CTTGATTGAC
    ACGTACCTGC CTGGAATTGA TGAAGAAACC AGTGAAGAAT CCCTCCTGAC TCCCACATCT
    CCTTACCCTC CTGCACTGCA GAGCCAGCTT AGTATCACTG CCAACCTTAA CCTTTCTAAT
    TCCATGACCT CACTTGCAAC TTCCCAGCAT TCCCCAGGAA TCGACAAGGA GAACGTTGAA
    CTCTCCCCTA CCACTGGCCA CTGTAACAGT GGACGAACTC GCCACGGATC CGCAAGCCAA
    GTGCAGAAGC AAAGAAGCGC TGGCAGTTTC AAACGTAATA GCATTAAGAA GATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035957.1
    CDS334..8853
    Translation

    Target ORF information:

    RefSeq Version NM_001042492.3
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042492.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
    CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
    GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
    CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
    GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
    TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
    TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
    GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
    CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
    GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
    AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
    ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
    GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
    ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
    TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
    GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
    ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
    GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
    GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
    GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
    GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
    ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
    GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
    GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
    CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
    GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
    CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
    CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
    GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
    TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
    CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
    CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
    TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
    GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
    CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
    GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
    CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
    CTTCGAAGTG TGTGCCACTG TTTATACCAG GCAACTTGCC ACTCCCTACT GAATAAAGCT
    ACAGTAAAAG AAAAAAAGGA AAACAAAAAA TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC
    AGCATCGGTG CAGTAGGAAG TGCCATGTTC CTCAGATTTA TCAATCCTGC CATTGTCTCA
    CCGTATGAAG CAGGGATTTT AGATAAAAAG CCACCACCTA GAATCGAAAG GGGCTTGAAG
    TTAATGTCAA AGATACTTCA GAGTATTGCC AATCATGTTC TCTTCACAAA AGAAGAACAT
    ATGCGGCCTT TCAATGATTT TGTGAAAAGC AACTTTGATG CAGCACGCAG GTTTTTCCTT
    GATATAGCAT CTGATTGTCC TACAAGTGAT GCAGTAAATC ATAGTCTTTC CTTCATAAGT
    GACGGCAATG TGCTTGCTTT ACATCGTCTA CTCTGGAACA ATCAGGAGAA AATTGGGCAG
    TATCTTTCCA GCAACAGGGA TCATAAAGCT GTTGGAAGAC GACCTTTTGA TAAGATGGCA
    ACACTTCTTG CATACCTGGG TCCTCCAGAG CACAAACCTG TGGCAGATAC ACACTGGTCC
    AGCCTTAACC TTACCAGTTC AAAGTTTGAG GAATTTATGA CTAGGCATCA GGTACATGAA
    AAAGAAGAAT TCAAGGCTTT GAAAACGTTA AGTATTTTCT ACCAAGCTGG GACTTCCAAA
    GCTGGGAATC CTATTTTTTA TTATGTTGCA CGGAGGTTCA AAACTGGTCA AATCAATGGT
    GATTTGCTGA TATACCATGT CTTACTGACT TTAAAGCCAT ATTATGCAAA GCCATATGAA
    ATTGTAGTGG ACCTTACCCA TACCGGGCCT AGCAATCGCT TTAAAACAGA CTTTCTCTCT
    AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT TACGACAACG TCTCCGCAGT CTATATCTAT
    AACTGTAACT CCTGGGTCAG GGAGTACACC AAGTATCATG AGCGGCTGCT GACTGGCCTC
    AAAGGTAGCA AAAGGCTTGT TTTCATAGAC TGTCCTGGGA AACTGGCTGA GCACATAGAG
    CATGAACAAC AGAAACTACC TGCTGCCACC TTGGCTTTAG AAGAGGACCT GAAGGTATTC
    CACAATGCTC TCAAGCTAGC TCACAAAGAC ACCAAAGTTT CTATTAAAGT TGGTTCTACT
    GCTGTCCAAG TAACTTCAGC AGAGCGAACA AAAGTCCTAG GGCAATCAGT CTTTCTAAAT
    GACATTTATT ATGCTTCGGA AATTGAAGAA ATCTGCCTAG TAGATGAGAA CCAGTTCACC
    TTAACCATTG CAAACCAGGG CACGCCGCTC ACCTTCATGC ACCAGGAGTG TGAAGCCATT
    GTCCAGTCTA TCATTCATAT CCGGACCCGC TGGGAACTGT CACAGCCCGA CTCTATCCCC
    CAACACACCA AGATTCGGCC AAAAGATGTC CCTGGGACAC TGCTCAATAT CGCATTACTT
    AATTTAGGCA GTTCTGACCC GAGTTTACGG TCAGCTGCCT ATAATCTTCT GTGTGCCTTA
    ACTTGTACCT TTAATTTAAA AATCGAGGGC CAGTTACTAG AGACATCAGG TTTATGTATC
    CCTGCCAACA ACACCCTCTT TATTGTCTCT ATTAGTAAGA CACTGGCAGC CAATGAGCCA
    CACCTCACGT TAGAATTTTT GGAAGAGTGT ATTTCTGGAT TTAGCAAATC TAGTATTGAA
    TTGAAACACC TTTGTTTGGA ATACATGACT CCATGGCTGT CAAATCTAGT TCGTTTTTGC
    AAGCATAATG ATGATGCCAA ACGACAAAGA GTTACTGCTA TTCTTGACAA GCTGATAACA
    ATGACCATCA ATGAAAAACA GATGTACCCA TCTATTCAAG CAAAAATATG GGGAAGCCTT
    GGGCAGATTA CAGATCTGCT TGATGTTGTA CTAGACAGTT TCATCAAAAC CAGTGCAACA
    GGTGGCTTGG GATCAATAAA AGCTGAGGTG ATGGCAGATA CTGCTGTAGC TTTGGCTTCT
    GGAAATGTGA AATTGGTTTC AAGCAAGGTT ATTGGAAGGA TGTGCAAAAT AATTGACAAG
    ACATGCTTAT CTCCAACTCC TACTTTAGAA CAACATCTTA TGTGGGATGA TATTGCTATT
    TTAGCACGCT ACATGCTGAT GCTGTCCTTC AACAATTCCC TTGATGTGGC AGCTCATCTT
    CCCTACCTCT TCCACGTTGT TACTTTCTTA GTAGCCACAG GTCCGCTCTC CCTTAGAGCT
    TCCACACATG GACTGGTCAT TAATATCATT CACTCTCTGT GTACTTGTTC ACAGCTTCAT
    TTTAGTGAAG AGACCAAGCA AGTTTTGAGA CTCAGTCTGA CAGAGTTCTC ATTACCCAAA
    TTTTACTTGC TGTTTGGCAT TAGCAAAGTC AAGTCAGCTG CTGTCATTGC CTTCCGTTCC
    AGTTACCGGG ACAGGTCATT CTCTCCTGGC TCCTATGAGA GAGAGACTTT TGCTTTGACA
    TCCTTGGAAA CAGTCACAGA AGCTTTGTTG GAGATCATGG AGGCATGCAT GAGAGATATT
    CCAACGTGCA AGTGGCTGGA CCAGTGGACA GAACTAGCTC AAAGATTTGC ATTCCAATAT
    AATCCATCCC TGCAACCAAG AGCTCTTGTT GTCTTTGGGT GTATTAGCAA ACGAGTGTCT
    CATGGGCAGA TAAAGCAGAT AATCCGTATT CTTAGCAAGG CACTTGAGAG TTGCTTAAAA
    GGACCTGACA CTTACAACAG TCAAGTTCTG ATAGAAGCTA CAGTAATAGC ACTAACCAAA
    TTACAGCCAC TTCTTAATAA GGACTCGCCT CTGCACAAAG CCCTCTTTTG GGTAGCTGTG
    GCTGTGCTGC AGCTTGATGA GGTCAACTTG TATTCAGCAG GTACCGCACT TCTTGAACAA
    AACCTGCATA CTTTAGATAG TCTCCGTATA TTCAATGACA AGAGTCCAGA GGAAGTATTT
    ATGGCAATCC GGAATCCTCT GGAGTGGCAC TGCAAGCAAA TGGATCATTT TGTTGGACTC
    AATTTCAACT CTAACTTTAA CTTTGCATTG GTTGGACACC TTTTAAAAGG GTACAGGCAT
    CCTTCACCTG CTATTGTTGC AAGAACAGTC AGAATTTTAC ATACACTACT AACTCTGGTT
    AACAAACACA GAAATTGTGA CAAATTTGAA GTGAATACAC AGAGCGTGGC CTACTTAGCA
    GCTTTACTTA CAGTGTCTGA AGAAGTTCGA AGTCGCTGCA GCCTAAAACA TAGAAAGTCA
    CTTCTTCTTA CTGATATTTC AATGGAAAAT GTTCCTATGG ATACATATCC CATTCATCAT
    GGTGACCCTT CCTATAGGAC ACTAAAGGAG ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT
    GAAGGATACC TTGCAGCCAC CTATCCAACT GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA
    TCCATGAGCC TGGACATGGG GCAACCTTCT CAGGCCAACA CTAAGAAGTT GCTTGGAACA
    AGGAAAAGTT TTGATCACTT GATATCAGAC ACAAAGGCTC CTAAAAGGCA AGAAATGGAA
    TCAGGGATCA CAACACCCCC CAAAATGAGG AGAGTAGCAG AAACTGATTA TGAAATGGAA
    ACTCAGAGGA TTTCCTCATC ACAACAGCAC CCACATTTAC GTAAAGTTTC AGTGTCTGAA
    TCAAATGTTC TCTTGGATGA AGAAGTACTT ACTGATCCGA AGATCCAGGC GCTGCTTCTT
    ACTGTTCTAG CTACACTGGT AAAATATACC ACAGATGAGT TTGATCAACG AATTCTTTAT
    GAATACTTAG CAGAGGCCAG TGTTGTGTTT CCCAAAGTCT TTCCTGTTGT GCATAATTTG
    TTGGACTCTA AGATCAACAC CCTGTTATCA TTGTGCCAAG ATCCAAATTT GTTAAATCCA
    ATCCATGGAA TTGTGCAGAG TGTGGTGTAC CATGAAGAAT CCCCACCACA ATACCAAACA
    TCTTACCTGC AAAGTTTTGG TTTTAATGGC TTGTGGCGGT TTGCAGGACC GTTTTCAAAG
    CAAACACAAA TTCCAGACTA TGCTGAGCTT ATTGTTAAGT TTCTTGATGC CTTGATTGAC
    ACGTACCTGC CTGGAATTGA TGAAGAAACC AGTGAAGAAT CCCTCCTGAC TCCCACATCT
    CCTTACCCTC CTGCACTGCA GAGCCAGCTT AGTATCACTG CCAACCTTAA CCTTTCTAAT
    TCCATGACCT CACTTGCAAC TTCCCAGCAT TCCCCAGGAA TCGACAAGGA GAACGTTGAA
    CTCTCCCCTA CCACTGGCCA CTGTAACAGT GGACGAACTC GCCACGGATC CGCAAGCCAA
    GTGCAGAAGC AAAGAAGCGC TGGCAGTTTC AAACGTAATA GCATTAAGAA GATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23641
    Clone ID Related Accession (Same CDS sequence) NM_001042492.3 , NM_001042492.2
    Accession Version NM_001042492.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-15
    Organism Homo sapiens(human)
    Product neurofibromin isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D12625.1, M89914.1, BX391799.2, BP271334.1, AC135724.9 and AK026658.1. This sequence is a reference standard in the RefSeqGene project. On Dec 3, 2009 this sequence version replaced NM_001042492.1. Transcript Variant: This variant (1), with an additional in-frame coding exon, represents the longest transcript and encodes the longest isoform (1). Studies suggest preferential C->U RNA editing of transcripts containing this exon. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M89914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein undergoes RNA editing :: PMID: 8602361, 11727199 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
    CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
    GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
    CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
    GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
    TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
    TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
    GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
    CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
    GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
    AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
    ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
    GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
    ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
    TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
    GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
    ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
    GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
    GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
    GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
    GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
    ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
    GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
    GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
    CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
    GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
    CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
    CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
    GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
    TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
    CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
    CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
    TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
    GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
    CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
    GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
    CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
    CTTCGAAGTG TGTGCCACTG TTTATACCAG GCAACTTGCC ACTCCCTACT GAATAAAGCT
    ACAGTAAAAG AAAAAAAGGA AAACAAAAAA TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC
    AGCATCGGTG CAGTAGGAAG TGCCATGTTC CTCAGATTTA TCAATCCTGC CATTGTCTCA
    CCGTATGAAG CAGGGATTTT AGATAAAAAG CCACCACCTA GAATCGAAAG GGGCTTGAAG
    TTAATGTCAA AGATACTTCA GAGTATTGCC AATCATGTTC TCTTCACAAA AGAAGAACAT
    ATGCGGCCTT TCAATGATTT TGTGAAAAGC AACTTTGATG CAGCACGCAG GTTTTTCCTT
    GATATAGCAT CTGATTGTCC TACAAGTGAT GCAGTAAATC ATAGTCTTTC CTTCATAAGT
    GACGGCAATG TGCTTGCTTT ACATCGTCTA CTCTGGAACA ATCAGGAGAA AATTGGGCAG
    TATCTTTCCA GCAACAGGGA TCATAAAGCT GTTGGAAGAC GACCTTTTGA TAAGATGGCA
    ACACTTCTTG CATACCTGGG TCCTCCAGAG CACAAACCTG TGGCAGATAC ACACTGGTCC
    AGCCTTAACC TTACCAGTTC AAAGTTTGAG GAATTTATGA CTAGGCATCA GGTACATGAA
    AAAGAAGAAT TCAAGGCTTT GAAAACGTTA AGTATTTTCT ACCAAGCTGG GACTTCCAAA
    GCTGGGAATC CTATTTTTTA TTATGTTGCA CGGAGGTTCA AAACTGGTCA AATCAATGGT
    GATTTGCTGA TATACCATGT CTTACTGACT TTAAAGCCAT ATTATGCAAA GCCATATGAA
    ATTGTAGTGG ACCTTACCCA TACCGGGCCT AGCAATCGCT TTAAAACAGA CTTTCTCTCT
    AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT TACGACAACG TCTCCGCAGT CTATATCTAT
    AACTGTAACT CCTGGGTCAG GGAGTACACC AAGTATCATG AGCGGCTGCT GACTGGCCTC
    AAAGGTAGCA AAAGGCTTGT TTTCATAGAC TGTCCTGGGA AACTGGCTGA GCACATAGAG
    CATGAACAAC AGAAACTACC TGCTGCCACC TTGGCTTTAG AAGAGGACCT GAAGGTATTC
    CACAATGCTC TCAAGCTAGC TCACAAAGAC ACCAAAGTTT CTATTAAAGT TGGTTCTACT
    GCTGTCCAAG TAACTTCAGC AGAGCGAACA AAAGTCCTAG GGCAATCAGT CTTTCTAAAT
    GACATTTATT ATGCTTCGGA AATTGAAGAA ATCTGCCTAG TAGATGAGAA CCAGTTCACC
    TTAACCATTG CAAACCAGGG CACGCCGCTC ACCTTCATGC ACCAGGAGTG TGAAGCCATT
    GTCCAGTCTA TCATTCATAT CCGGACCCGC TGGGAACTGT CACAGCCCGA CTCTATCCCC
    CAACACACCA AGATTCGGCC AAAAGATGTC CCTGGGACAC TGCTCAATAT CGCATTACTT
    AATTTAGGCA GTTCTGACCC GAGTTTACGG TCAGCTGCCT ATAATCTTCT GTGTGCCTTA
    ACTTGTACCT TTAATTTAAA AATCGAGGGC CAGTTACTAG AGACATCAGG TTTATGTATC
    CCTGCCAACA ACACCCTCTT TATTGTCTCT ATTAGTAAGA CACTGGCAGC CAATGAGCCA
    CACCTCACGT TAGAATTTTT GGAAGAGTGT ATTTCTGGAT TTAGCAAATC TAGTATTGAA
    TTGAAACACC TTTGTTTGGA ATACATGACT CCATGGCTGT CAAATCTAGT TCGTTTTTGC
    AAGCATAATG ATGATGCCAA ACGACAAAGA GTTACTGCTA TTCTTGACAA GCTGATAACA
    ATGACCATCA ATGAAAAACA GATGTACCCA TCTATTCAAG CAAAAATATG GGGAAGCCTT
    GGGCAGATTA CAGATCTGCT TGATGTTGTA CTAGACAGTT TCATCAAAAC CAGTGCAACA
    GGTGGCTTGG GATCAATAAA AGCTGAGGTG ATGGCAGATA CTGCTGTAGC TTTGGCTTCT
    GGAAATGTGA AATTGGTTTC AAGCAAGGTT ATTGGAAGGA TGTGCAAAAT AATTGACAAG
    ACATGCTTAT CTCCAACTCC TACTTTAGAA CAACATCTTA TGTGGGATGA TATTGCTATT
    TTAGCACGCT ACATGCTGAT GCTGTCCTTC AACAATTCCC TTGATGTGGC AGCTCATCTT
    CCCTACCTCT TCCACGTTGT TACTTTCTTA GTAGCCACAG GTCCGCTCTC CCTTAGAGCT
    TCCACACATG GACTGGTCAT TAATATCATT CACTCTCTGT GTACTTGTTC ACAGCTTCAT
    TTTAGTGAAG AGACCAAGCA AGTTTTGAGA CTCAGTCTGA CAGAGTTCTC ATTACCCAAA
    TTTTACTTGC TGTTTGGCAT TAGCAAAGTC AAGTCAGCTG CTGTCATTGC CTTCCGTTCC
    AGTTACCGGG ACAGGTCATT CTCTCCTGGC TCCTATGAGA GAGAGACTTT TGCTTTGACA
    TCCTTGGAAA CAGTCACAGA AGCTTTGTTG GAGATCATGG AGGCATGCAT GAGAGATATT
    CCAACGTGCA AGTGGCTGGA CCAGTGGACA GAACTAGCTC AAAGATTTGC ATTCCAATAT
    AATCCATCCC TGCAACCAAG AGCTCTTGTT GTCTTTGGGT GTATTAGCAA ACGAGTGTCT
    CATGGGCAGA TAAAGCAGAT AATCCGTATT CTTAGCAAGG CACTTGAGAG TTGCTTAAAA
    GGACCTGACA CTTACAACAG TCAAGTTCTG ATAGAAGCTA CAGTAATAGC ACTAACCAAA
    TTACAGCCAC TTCTTAATAA GGACTCGCCT CTGCACAAAG CCCTCTTTTG GGTAGCTGTG
    GCTGTGCTGC AGCTTGATGA GGTCAACTTG TATTCAGCAG GTACCGCACT TCTTGAACAA
    AACCTGCATA CTTTAGATAG TCTCCGTATA TTCAATGACA AGAGTCCAGA GGAAGTATTT
    ATGGCAATCC GGAATCCTCT GGAGTGGCAC TGCAAGCAAA TGGATCATTT TGTTGGACTC
    AATTTCAACT CTAACTTTAA CTTTGCATTG GTTGGACACC TTTTAAAAGG GTACAGGCAT
    CCTTCACCTG CTATTGTTGC AAGAACAGTC AGAATTTTAC ATACACTACT AACTCTGGTT
    AACAAACACA GAAATTGTGA CAAATTTGAA GTGAATACAC AGAGCGTGGC CTACTTAGCA
    GCTTTACTTA CAGTGTCTGA AGAAGTTCGA AGTCGCTGCA GCCTAAAACA TAGAAAGTCA
    CTTCTTCTTA CTGATATTTC AATGGAAAAT GTTCCTATGG ATACATATCC CATTCATCAT
    GGTGACCCTT CCTATAGGAC ACTAAAGGAG ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT
    GAAGGATACC TTGCAGCCAC CTATCCAACT GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA
    TCCATGAGCC TGGACATGGG GCAACCTTCT CAGGCCAACA CTAAGAAGTT GCTTGGAACA
    AGGAAAAGTT TTGATCACTT GATATCAGAC ACAAAGGCTC CTAAAAGGCA AGAAATGGAA
    TCAGGGATCA CAACACCCCC CAAAATGAGG AGAGTAGCAG AAACTGATTA TGAAATGGAA
    ACTCAGAGGA TTTCCTCATC ACAACAGCAC CCACATTTAC GTAAAGTTTC AGTGTCTGAA
    TCAAATGTTC TCTTGGATGA AGAAGTACTT ACTGATCCGA AGATCCAGGC GCTGCTTCTT
    ACTGTTCTAG CTACACTGGT AAAATATACC ACAGATGAGT TTGATCAACG AATTCTTTAT
    GAATACTTAG CAGAGGCCAG TGTTGTGTTT CCCAAAGTCT TTCCTGTTGT GCATAATTTG
    TTGGACTCTA AGATCAACAC CCTGTTATCA TTGTGCCAAG ATCCAAATTT GTTAAATCCA
    ATCCATGGAA TTGTGCAGAG TGTGGTGTAC CATGAAGAAT CCCCACCACA ATACCAAACA
    TCTTACCTGC AAAGTTTTGG TTTTAATGGC TTGTGGCGGT TTGCAGGACC GTTTTCAAAG
    CAAACACAAA TTCCAGACTA TGCTGAGCTT ATTGTTAAGT TTCTTGATGC CTTGATTGAC
    ACGTACCTGC CTGGAATTGA TGAAGAAACC AGTGAAGAAT CCCTCCTGAC TCCCACATCT
    CCTTACCCTC CTGCACTGCA GAGCCAGCTT AGTATCACTG CCAACCTTAA CCTTTCTAAT
    TCCATGACCT CACTTGCAAC TTCCCAGCAT TCCCCAGGAA TCGACAAGGA GAACGTTGAA
    CTCTCCCCTA CCACTGGCCA CTGTAACAGT GGACGAACTC GCCACGGATC CGCAAGCCAA
    GTGCAGAAGC AAAGAAGCGC TGGCAGTTTC AAACGTAATA GCATTAAGAA GATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001035957.1
    CDS384..8903
    Translation

    Target ORF information:

    RefSeq Version NM_001042492.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001042492.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
    CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
    GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
    CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
    GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
    TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
    TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
    GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
    CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
    GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
    AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
    ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
    GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
    ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
    TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
    GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
    ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
    GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
    GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
    GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
    GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
    ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
    GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
    GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
    CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
    GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
    CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
    CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
    GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
    TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
    CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
    CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
    TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
    GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
    CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
    GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
    CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
    CTTCGAAGTG TGTGCCACTG TTTATACCAG GCAACTTGCC ACTCCCTACT GAATAAAGCT
    ACAGTAAAAG AAAAAAAGGA AAACAAAAAA TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC
    AGCATCGGTG CAGTAGGAAG TGCCATGTTC CTCAGATTTA TCAATCCTGC CATTGTCTCA
    CCGTATGAAG CAGGGATTTT AGATAAAAAG CCACCACCTA GAATCGAAAG GGGCTTGAAG
    TTAATGTCAA AGATACTTCA GAGTATTGCC AATCATGTTC TCTTCACAAA AGAAGAACAT
    ATGCGGCCTT TCAATGATTT TGTGAAAAGC AACTTTGATG CAGCACGCAG GTTTTTCCTT
    GATATAGCAT CTGATTGTCC TACAAGTGAT GCAGTAAATC ATAGTCTTTC CTTCATAAGT
    GACGGCAATG TGCTTGCTTT ACATCGTCTA CTCTGGAACA ATCAGGAGAA AATTGGGCAG
    TATCTTTCCA GCAACAGGGA TCATAAAGCT GTTGGAAGAC GACCTTTTGA TAAGATGGCA
    ACACTTCTTG CATACCTGGG TCCTCCAGAG CACAAACCTG TGGCAGATAC ACACTGGTCC
    AGCCTTAACC TTACCAGTTC AAAGTTTGAG GAATTTATGA CTAGGCATCA GGTACATGAA
    AAAGAAGAAT TCAAGGCTTT GAAAACGTTA AGTATTTTCT ACCAAGCTGG GACTTCCAAA
    GCTGGGAATC CTATTTTTTA TTATGTTGCA CGGAGGTTCA AAACTGGTCA AATCAATGGT
    GATTTGCTGA TATACCATGT CTTACTGACT TTAAAGCCAT ATTATGCAAA GCCATATGAA
    ATTGTAGTGG ACCTTACCCA TACCGGGCCT AGCAATCGCT TTAAAACAGA CTTTCTCTCT
    AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT TACGACAACG TCTCCGCAGT CTATATCTAT
    AACTGTAACT CCTGGGTCAG GGAGTACACC AAGTATCATG AGCGGCTGCT GACTGGCCTC
    AAAGGTAGCA AAAGGCTTGT TTTCATAGAC TGTCCTGGGA AACTGGCTGA GCACATAGAG
    CATGAACAAC AGAAACTACC TGCTGCCACC TTGGCTTTAG AAGAGGACCT GAAGGTATTC
    CACAATGCTC TCAAGCTAGC TCACAAAGAC ACCAAAGTTT CTATTAAAGT TGGTTCTACT
    GCTGTCCAAG TAACTTCAGC AGAGCGAACA AAAGTCCTAG GGCAATCAGT CTTTCTAAAT
    GACATTTATT ATGCTTCGGA AATTGAAGAA ATCTGCCTAG TAGATGAGAA CCAGTTCACC
    TTAACCATTG CAAACCAGGG CACGCCGCTC ACCTTCATGC ACCAGGAGTG TGAAGCCATT
    GTCCAGTCTA TCATTCATAT CCGGACCCGC TGGGAACTGT CACAGCCCGA CTCTATCCCC
    CAACACACCA AGATTCGGCC AAAAGATGTC CCTGGGACAC TGCTCAATAT CGCATTACTT
    AATTTAGGCA GTTCTGACCC GAGTTTACGG TCAGCTGCCT ATAATCTTCT GTGTGCCTTA
    ACTTGTACCT TTAATTTAAA AATCGAGGGC CAGTTACTAG AGACATCAGG TTTATGTATC
    CCTGCCAACA ACACCCTCTT TATTGTCTCT ATTAGTAAGA CACTGGCAGC CAATGAGCCA
    CACCTCACGT TAGAATTTTT GGAAGAGTGT ATTTCTGGAT TTAGCAAATC TAGTATTGAA
    TTGAAACACC TTTGTTTGGA ATACATGACT CCATGGCTGT CAAATCTAGT TCGTTTTTGC
    AAGCATAATG ATGATGCCAA ACGACAAAGA GTTACTGCTA TTCTTGACAA GCTGATAACA
    ATGACCATCA ATGAAAAACA GATGTACCCA TCTATTCAAG CAAAAATATG GGGAAGCCTT
    GGGCAGATTA CAGATCTGCT TGATGTTGTA CTAGACAGTT TCATCAAAAC CAGTGCAACA
    GGTGGCTTGG GATCAATAAA AGCTGAGGTG ATGGCAGATA CTGCTGTAGC TTTGGCTTCT
    GGAAATGTGA AATTGGTTTC AAGCAAGGTT ATTGGAAGGA TGTGCAAAAT AATTGACAAG
    ACATGCTTAT CTCCAACTCC TACTTTAGAA CAACATCTTA TGTGGGATGA TATTGCTATT
    TTAGCACGCT ACATGCTGAT GCTGTCCTTC AACAATTCCC TTGATGTGGC AGCTCATCTT
    CCCTACCTCT TCCACGTTGT TACTTTCTTA GTAGCCACAG GTCCGCTCTC CCTTAGAGCT
    TCCACACATG GACTGGTCAT TAATATCATT CACTCTCTGT GTACTTGTTC ACAGCTTCAT
    TTTAGTGAAG AGACCAAGCA AGTTTTGAGA CTCAGTCTGA CAGAGTTCTC ATTACCCAAA
    TTTTACTTGC TGTTTGGCAT TAGCAAAGTC AAGTCAGCTG CTGTCATTGC CTTCCGTTCC
    AGTTACCGGG ACAGGTCATT CTCTCCTGGC TCCTATGAGA GAGAGACTTT TGCTTTGACA
    TCCTTGGAAA CAGTCACAGA AGCTTTGTTG GAGATCATGG AGGCATGCAT GAGAGATATT
    CCAACGTGCA AGTGGCTGGA CCAGTGGACA GAACTAGCTC AAAGATTTGC ATTCCAATAT
    AATCCATCCC TGCAACCAAG AGCTCTTGTT GTCTTTGGGT GTATTAGCAA ACGAGTGTCT
    CATGGGCAGA TAAAGCAGAT AATCCGTATT CTTAGCAAGG CACTTGAGAG TTGCTTAAAA
    GGACCTGACA CTTACAACAG TCAAGTTCTG ATAGAAGCTA CAGTAATAGC ACTAACCAAA
    TTACAGCCAC TTCTTAATAA GGACTCGCCT CTGCACAAAG CCCTCTTTTG GGTAGCTGTG
    GCTGTGCTGC AGCTTGATGA GGTCAACTTG TATTCAGCAG GTACCGCACT TCTTGAACAA
    AACCTGCATA CTTTAGATAG TCTCCGTATA TTCAATGACA AGAGTCCAGA GGAAGTATTT
    ATGGCAATCC GGAATCCTCT GGAGTGGCAC TGCAAGCAAA TGGATCATTT TGTTGGACTC
    AATTTCAACT CTAACTTTAA CTTTGCATTG GTTGGACACC TTTTAAAAGG GTACAGGCAT
    CCTTCACCTG CTATTGTTGC AAGAACAGTC AGAATTTTAC ATACACTACT AACTCTGGTT
    AACAAACACA GAAATTGTGA CAAATTTGAA GTGAATACAC AGAGCGTGGC CTACTTAGCA
    GCTTTACTTA CAGTGTCTGA AGAAGTTCGA AGTCGCTGCA GCCTAAAACA TAGAAAGTCA
    CTTCTTCTTA CTGATATTTC AATGGAAAAT GTTCCTATGG ATACATATCC CATTCATCAT
    GGTGACCCTT CCTATAGGAC ACTAAAGGAG ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT
    GAAGGATACC TTGCAGCCAC CTATCCAACT GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA
    TCCATGAGCC TGGACATGGG GCAACCTTCT CAGGCCAACA CTAAGAAGTT GCTTGGAACA
    AGGAAAAGTT TTGATCACTT GATATCAGAC ACAAAGGCTC CTAAAAGGCA AGAAATGGAA
    TCAGGGATCA CAACACCCCC CAAAATGAGG AGAGTAGCAG AAACTGATTA TGAAATGGAA
    ACTCAGAGGA TTTCCTCATC ACAACAGCAC CCACATTTAC GTAAAGTTTC AGTGTCTGAA
    TCAAATGTTC TCTTGGATGA AGAAGTACTT ACTGATCCGA AGATCCAGGC GCTGCTTCTT
    ACTGTTCTAG CTACACTGGT AAAATATACC ACAGATGAGT TTGATCAACG AATTCTTTAT
    GAATACTTAG CAGAGGCCAG TGTTGTGTTT CCCAAAGTCT TTCCTGTTGT GCATAATTTG
    TTGGACTCTA AGATCAACAC CCTGTTATCA TTGTGCCAAG ATCCAAATTT GTTAAATCCA
    ATCCATGGAA TTGTGCAGAG TGTGGTGTAC CATGAAGAAT CCCCACCACA ATACCAAACA
    TCTTACCTGC AAAGTTTTGG TTTTAATGGC TTGTGGCGGT TTGCAGGACC GTTTTCAAAG
    CAAACACAAA TTCCAGACTA TGCTGAGCTT ATTGTTAAGT TTCTTGATGC CTTGATTGAC
    ACGTACCTGC CTGGAATTGA TGAAGAAACC AGTGAAGAAT CCCTCCTGAC TCCCACATCT
    CCTTACCCTC CTGCACTGCA GAGCCAGCTT AGTATCACTG CCAACCTTAA CCTTTCTAAT
    TCCATGACCT CACTTGCAAC TTCCCAGCAT TCCCCAGGAA TCGACAAGGA GAACGTTGAA
    CTCTCCCCTA CCACTGGCCA CTGTAACAGT GGACGAACTC GCCACGGATC CGCAAGCCAA
    GTGCAGAAGC AAAGAAGCGC TGGCAGTTTC AAACGTAATA GCATTAAGAA GATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24060
    Clone ID Related Accession (Same CDS sequence) NM_000267.3
    Accession Version NM_000267.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8457bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product neurofibromin isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D12625.1, M82814.1, CN415204.1, AC135724.9 and AK026658.1. This sequence is a reference standard in the RefSeqGene project. On Dec 3, 2009 this sequence version replaced NM_000267.2. Transcript Variant: This variant (2) lacks an in-frame coding exon compared to transcript variant 1, resulting in a shorter isoform (2) missing an internal 21 aa segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M82814.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## undergoes RNA editing :: PMID: 8602361, 11727199 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
    CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
    GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
    CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
    GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
    TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
    TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
    GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
    CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
    GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
    AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
    ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
    GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
    ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
    TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
    GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
    ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
    GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
    GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
    GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
    GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
    ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
    GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
    GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
    CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
    GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
    CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
    CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
    GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
    TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
    CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
    CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
    TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
    GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
    CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
    GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
    CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
    CTTCGAAGTG TGTGCCACTG TTTATACCAG GTGGTTAGCC AGCGTTTCCC TCAGAACAGC
    ATCGGTGCAG TAGGAAGTGC CATGTTCCTC AGATTTATCA ATCCTGCCAT TGTCTCACCG
    TATGAAGCAG GGATTTTAGA TAAAAAGCCA CCACCTAGAA TCGAAAGGGG CTTGAAGTTA
    ATGTCAAAGA TACTTCAGAG TATTGCCAAT CATGTTCTCT TCACAAAAGA AGAACATATG
    CGGCCTTTCA ATGATTTTGT GAAAAGCAAC TTTGATGCAG CACGCAGGTT TTTCCTTGAT
    ATAGCATCTG ATTGTCCTAC AAGTGATGCA GTAAATCATA GTCTTTCCTT CATAAGTGAC
    GGCAATGTGC TTGCTTTACA TCGTCTACTC TGGAACAATC AGGAGAAAAT TGGGCAGTAT
    CTTTCCAGCA ACAGGGATCA TAAAGCTGTT GGAAGACGAC CTTTTGATAA GATGGCAACA
    CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC AAACCTGTGG CAGATACACA CTGGTCCAGC
    CTTAACCTTA CCAGTTCAAA GTTTGAGGAA TTTATGACTA GGCATCAGGT ACATGAAAAA
    GAAGAATTCA AGGCTTTGAA AACGTTAAGT ATTTTCTACC AAGCTGGGAC TTCCAAAGCT
    GGGAATCCTA TTTTTTATTA TGTTGCACGG AGGTTCAAAA CTGGTCAAAT CAATGGTGAT
    TTGCTGATAT ACCATGTCTT ACTGACTTTA AAGCCATATT ATGCAAAGCC ATATGAAATT
    GTAGTGGACC TTACCCATAC CGGGCCTAGC AATCGCTTTA AAACAGACTT TCTCTCTAAG
    TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC GACAACGTCT CCGCAGTCTA TATCTATAAC
    TGTAACTCCT GGGTCAGGGA GTACACCAAG TATCATGAGC GGCTGCTGAC TGGCCTCAAA
    GGTAGCAAAA GGCTTGTTTT CATAGACTGT CCTGGGAAAC TGGCTGAGCA CATAGAGCAT
    GAACAACAGA AACTACCTGC TGCCACCTTG GCTTTAGAAG AGGACCTGAA GGTATTCCAC
    AATGCTCTCA AGCTAGCTCA CAAAGACACC AAAGTTTCTA TTAAAGTTGG TTCTACTGCT
    GTCCAAGTAA CTTCAGCAGA GCGAACAAAA GTCCTAGGGC AATCAGTCTT TCTAAATGAC
    ATTTATTATG CTTCGGAAAT TGAAGAAATC TGCCTAGTAG ATGAGAACCA GTTCACCTTA
    ACCATTGCAA ACCAGGGCAC GCCGCTCACC TTCATGCACC AGGAGTGTGA AGCCATTGTC
    CAGTCTATCA TTCATATCCG GACCCGCTGG GAACTGTCAC AGCCCGACTC TATCCCCCAA
    CACACCAAGA TTCGGCCAAA AGATGTCCCT GGGACACTGC TCAATATCGC ATTACTTAAT
    TTAGGCAGTT CTGACCCGAG TTTACGGTCA GCTGCCTATA ATCTTCTGTG TGCCTTAACT
    TGTACCTTTA ATTTAAAAAT CGAGGGCCAG TTACTAGAGA CATCAGGTTT ATGTATCCCT
    GCCAACAACA CCCTCTTTAT TGTCTCTATT AGTAAGACAC TGGCAGCCAA TGAGCCACAC
    CTCACGTTAG AATTTTTGGA AGAGTGTATT TCTGGATTTA GCAAATCTAG TATTGAATTG
    AAACACCTTT GTTTGGAATA CATGACTCCA TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG
    CATAATGATG ATGCCAAACG ACAAAGAGTT ACTGCTATTC TTGACAAGCT GATAACAATG
    ACCATCAATG AAAAACAGAT GTACCCATCT ATTCAAGCAA AAATATGGGG AAGCCTTGGG
    CAGATTACAG ATCTGCTTGA TGTTGTACTA GACAGTTTCA TCAAAACCAG TGCAACAGGT
    GGCTTGGGAT CAATAAAAGC TGAGGTGATG GCAGATACTG CTGTAGCTTT GGCTTCTGGA
    AATGTGAAAT TGGTTTCAAG CAAGGTTATT GGAAGGATGT GCAAAATAAT TGACAAGACA
    TGCTTATCTC CAACTCCTAC TTTAGAACAA CATCTTATGT GGGATGATAT TGCTATTTTA
    GCACGCTACA TGCTGATGCT GTCCTTCAAC AATTCCCTTG ATGTGGCAGC TCATCTTCCC
    TACCTCTTCC ACGTTGTTAC TTTCTTAGTA GCCACAGGTC CGCTCTCCCT TAGAGCTTCC
    ACACATGGAC TGGTCATTAA TATCATTCAC TCTCTGTGTA CTTGTTCACA GCTTCATTTT
    AGTGAAGAGA CCAAGCAAGT TTTGAGACTC AGTCTGACAG AGTTCTCATT ACCCAAATTT
    TACTTGCTGT TTGGCATTAG CAAAGTCAAG TCAGCTGCTG TCATTGCCTT CCGTTCCAGT
    TACCGGGACA GGTCATTCTC TCCTGGCTCC TATGAGAGAG AGACTTTTGC TTTGACATCC
    TTGGAAACAG TCACAGAAGC TTTGTTGGAG ATCATGGAGG CATGCATGAG AGATATTCCA
    ACGTGCAAGT GGCTGGACCA GTGGACAGAA CTAGCTCAAA GATTTGCATT CCAATATAAT
    CCATCCCTGC AACCAAGAGC TCTTGTTGTC TTTGGGTGTA TTAGCAAACG AGTGTCTCAT
    GGGCAGATAA AGCAGATAAT CCGTATTCTT AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA
    CCTGACACTT ACAACAGTCA AGTTCTGATA GAAGCTACAG TAATAGCACT AACCAAATTA
    CAGCCACTTC TTAATAAGGA CTCGCCTCTG CACAAAGCCC TCTTTTGGGT AGCTGTGGCT
    GTGCTGCAGC TTGATGAGGT CAACTTGTAT TCAGCAGGTA CCGCACTTCT TGAACAAAAC
    CTGCATACTT TAGATAGTCT CCGTATATTC AATGACAAGA GTCCAGAGGA AGTATTTATG
    GCAATCCGGA ATCCTCTGGA GTGGCACTGC AAGCAAATGG ATCATTTTGT TGGACTCAAT
    TTCAACTCTA ACTTTAACTT TGCATTGGTT GGACACCTTT TAAAAGGGTA CAGGCATCCT
    TCACCTGCTA TTGTTGCAAG AACAGTCAGA ATTTTACATA CACTACTAAC TCTGGTTAAC
    AAACACAGAA ATTGTGACAA ATTTGAAGTG AATACACAGA GCGTGGCCTA CTTAGCAGCT
    TTACTTACAG TGTCTGAAGA AGTTCGAAGT CGCTGCAGCC TAAAACATAG AAAGTCACTT
    CTTCTTACTG ATATTTCAAT GGAAAATGTT CCTATGGATA CATATCCCAT TCATCATGGT
    GACCCTTCCT ATAGGACACT AAAGGAGACT CAGCCATGGT CCTCTCCCAA AGGTTCTGAA
    GGATACCTTG CAGCCACCTA TCCAACTGTC GGCCAGACCA GTCCCCGAGC CAGGAAATCC
    ATGAGCCTGG ACATGGGGCA ACCTTCTCAG GCCAACACTA AGAAGTTGCT TGGAACAAGG
    AAAAGTTTTG ATCACTTGAT ATCAGACACA AAGGCTCCTA AAAGGCAAGA AATGGAATCA
    GGGATCACAA CACCCCCCAA AATGAGGAGA GTAGCAGAAA CTGATTATGA AATGGAAACT
    CAGAGGATTT CCTCATCACA ACAGCACCCA CATTTACGTA AAGTTTCAGT GTCTGAATCA
    AATGTTCTCT TGGATGAAGA AGTACTTACT GATCCGAAGA TCCAGGCGCT GCTTCTTACT
    GTTCTAGCTA CACTGGTAAA ATATACCACA GATGAGTTTG ATCAACGAAT TCTTTATGAA
    TACTTAGCAG AGGCCAGTGT TGTGTTTCCC AAAGTCTTTC CTGTTGTGCA TAATTTGTTG
    GACTCTAAGA TCAACACCCT GTTATCATTG TGCCAAGATC CAAATTTGTT AAATCCAATC
    CATGGAATTG TGCAGAGTGT GGTGTACCAT GAAGAATCCC CACCACAATA CCAAACATCT
    TACCTGCAAA GTTTTGGTTT TAATGGCTTG TGGCGGTTTG CAGGACCGTT TTCAAAGCAA
    ACACAAATTC CAGACTATGC TGAGCTTATT GTTAAGTTTC TTGATGCCTT GATTGACACG
    TACCTGCCTG GAATTGATGA AGAAACCAGT GAAGAATCCC TCCTGACTCC CACATCTCCT
    TACCCTCCTG CACTGCAGAG CCAGCTTAGT ATCACTGCCA ACCTTAACCT TTCTAATTCC
    ATGACCTCAC TTGCAACTTC CCAGCATTCC CCAGGAATCG ACAAGGAGAA CGTTGAACTC
    TCCCCTACCA CTGGCCACTG TAACAGTGGA CGAACTCGCC ACGGATCCGC AAGCCAAGTG
    CAGAAGCAAA GAAGCGCTGG CAGTTTCAAA CGTAATAGCA TTAAGAAGAT CGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000258.1
    CDS384..8840
    Translation

    Target ORF information:

    RefSeq Version NM_000267.3
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000267.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
    CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
    GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
    CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
    GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
    TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
    TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
    GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
    CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
    GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
    AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
    ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
    GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
    ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
    TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
    GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
    ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
    GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
    GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
    GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
    GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
    ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
    GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
    GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
    CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
    GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
    CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
    CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
    GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
    TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
    CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
    CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
    TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
    GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
    CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
    GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
    CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
    CTTCGAAGTG TGTGCCACTG TTTATACCAG GTGGTTAGCC AGCGTTTCCC TCAGAACAGC
    ATCGGTGCAG TAGGAAGTGC CATGTTCCTC AGATTTATCA ATCCTGCCAT TGTCTCACCG
    TATGAAGCAG GGATTTTAGA TAAAAAGCCA CCACCTAGAA TCGAAAGGGG CTTGAAGTTA
    ATGTCAAAGA TACTTCAGAG TATTGCCAAT CATGTTCTCT TCACAAAAGA AGAACATATG
    CGGCCTTTCA ATGATTTTGT GAAAAGCAAC TTTGATGCAG CACGCAGGTT TTTCCTTGAT
    ATAGCATCTG ATTGTCCTAC AAGTGATGCA GTAAATCATA GTCTTTCCTT CATAAGTGAC
    GGCAATGTGC TTGCTTTACA TCGTCTACTC TGGAACAATC AGGAGAAAAT TGGGCAGTAT
    CTTTCCAGCA ACAGGGATCA TAAAGCTGTT GGAAGACGAC CTTTTGATAA GATGGCAACA
    CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC AAACCTGTGG CAGATACACA CTGGTCCAGC
    CTTAACCTTA CCAGTTCAAA GTTTGAGGAA TTTATGACTA GGCATCAGGT ACATGAAAAA
    GAAGAATTCA AGGCTTTGAA AACGTTAAGT ATTTTCTACC AAGCTGGGAC TTCCAAAGCT
    GGGAATCCTA TTTTTTATTA TGTTGCACGG AGGTTCAAAA CTGGTCAAAT CAATGGTGAT
    TTGCTGATAT ACCATGTCTT ACTGACTTTA AAGCCATATT ATGCAAAGCC ATATGAAATT
    GTAGTGGACC TTACCCATAC CGGGCCTAGC AATCGCTTTA AAACAGACTT TCTCTCTAAG
    TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC GACAACGTCT CCGCAGTCTA TATCTATAAC
    TGTAACTCCT GGGTCAGGGA GTACACCAAG TATCATGAGC GGCTGCTGAC TGGCCTCAAA
    GGTAGCAAAA GGCTTGTTTT CATAGACTGT CCTGGGAAAC TGGCTGAGCA CATAGAGCAT
    GAACAACAGA AACTACCTGC TGCCACCTTG GCTTTAGAAG AGGACCTGAA GGTATTCCAC
    AATGCTCTCA AGCTAGCTCA CAAAGACACC AAAGTTTCTA TTAAAGTTGG TTCTACTGCT
    GTCCAAGTAA CTTCAGCAGA GCGAACAAAA GTCCTAGGGC AATCAGTCTT TCTAAATGAC
    ATTTATTATG CTTCGGAAAT TGAAGAAATC TGCCTAGTAG ATGAGAACCA GTTCACCTTA
    ACCATTGCAA ACCAGGGCAC GCCGCTCACC TTCATGCACC AGGAGTGTGA AGCCATTGTC
    CAGTCTATCA TTCATATCCG GACCCGCTGG GAACTGTCAC AGCCCGACTC TATCCCCCAA
    CACACCAAGA TTCGGCCAAA AGATGTCCCT GGGACACTGC TCAATATCGC ATTACTTAAT
    TTAGGCAGTT CTGACCCGAG TTTACGGTCA GCTGCCTATA ATCTTCTGTG TGCCTTAACT
    TGTACCTTTA ATTTAAAAAT CGAGGGCCAG TTACTAGAGA CATCAGGTTT ATGTATCCCT
    GCCAACAACA CCCTCTTTAT TGTCTCTATT AGTAAGACAC TGGCAGCCAA TGAGCCACAC
    CTCACGTTAG AATTTTTGGA AGAGTGTATT TCTGGATTTA GCAAATCTAG TATTGAATTG
    AAACACCTTT GTTTGGAATA CATGACTCCA TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG
    CATAATGATG ATGCCAAACG ACAAAGAGTT ACTGCTATTC TTGACAAGCT GATAACAATG
    ACCATCAATG AAAAACAGAT GTACCCATCT ATTCAAGCAA AAATATGGGG AAGCCTTGGG
    CAGATTACAG ATCTGCTTGA TGTTGTACTA GACAGTTTCA TCAAAACCAG TGCAACAGGT
    GGCTTGGGAT CAATAAAAGC TGAGGTGATG GCAGATACTG CTGTAGCTTT GGCTTCTGGA
    AATGTGAAAT TGGTTTCAAG CAAGGTTATT GGAAGGATGT GCAAAATAAT TGACAAGACA
    TGCTTATCTC CAACTCCTAC TTTAGAACAA CATCTTATGT GGGATGATAT TGCTATTTTA
    GCACGCTACA TGCTGATGCT GTCCTTCAAC AATTCCCTTG ATGTGGCAGC TCATCTTCCC
    TACCTCTTCC ACGTTGTTAC TTTCTTAGTA GCCACAGGTC CGCTCTCCCT TAGAGCTTCC
    ACACATGGAC TGGTCATTAA TATCATTCAC TCTCTGTGTA CTTGTTCACA GCTTCATTTT
    AGTGAAGAGA CCAAGCAAGT TTTGAGACTC AGTCTGACAG AGTTCTCATT ACCCAAATTT
    TACTTGCTGT TTGGCATTAG CAAAGTCAAG TCAGCTGCTG TCATTGCCTT CCGTTCCAGT
    TACCGGGACA GGTCATTCTC TCCTGGCTCC TATGAGAGAG AGACTTTTGC TTTGACATCC
    TTGGAAACAG TCACAGAAGC TTTGTTGGAG ATCATGGAGG CATGCATGAG AGATATTCCA
    ACGTGCAAGT GGCTGGACCA GTGGACAGAA CTAGCTCAAA GATTTGCATT CCAATATAAT
    CCATCCCTGC AACCAAGAGC TCTTGTTGTC TTTGGGTGTA TTAGCAAACG AGTGTCTCAT
    GGGCAGATAA AGCAGATAAT CCGTATTCTT AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA
    CCTGACACTT ACAACAGTCA AGTTCTGATA GAAGCTACAG TAATAGCACT AACCAAATTA
    CAGCCACTTC TTAATAAGGA CTCGCCTCTG CACAAAGCCC TCTTTTGGGT AGCTGTGGCT
    GTGCTGCAGC TTGATGAGGT CAACTTGTAT TCAGCAGGTA CCGCACTTCT TGAACAAAAC
    CTGCATACTT TAGATAGTCT CCGTATATTC AATGACAAGA GTCCAGAGGA AGTATTTATG
    GCAATCCGGA ATCCTCTGGA GTGGCACTGC AAGCAAATGG ATCATTTTGT TGGACTCAAT
    TTCAACTCTA ACTTTAACTT TGCATTGGTT GGACACCTTT TAAAAGGGTA CAGGCATCCT
    TCACCTGCTA TTGTTGCAAG AACAGTCAGA ATTTTACATA CACTACTAAC TCTGGTTAAC
    AAACACAGAA ATTGTGACAA ATTTGAAGTG AATACACAGA GCGTGGCCTA CTTAGCAGCT
    TTACTTACAG TGTCTGAAGA AGTTCGAAGT CGCTGCAGCC TAAAACATAG AAAGTCACTT
    CTTCTTACTG ATATTTCAAT GGAAAATGTT CCTATGGATA CATATCCCAT TCATCATGGT
    GACCCTTCCT ATAGGACACT AAAGGAGACT CAGCCATGGT CCTCTCCCAA AGGTTCTGAA
    GGATACCTTG CAGCCACCTA TCCAACTGTC GGCCAGACCA GTCCCCGAGC CAGGAAATCC
    ATGAGCCTGG ACATGGGGCA ACCTTCTCAG GCCAACACTA AGAAGTTGCT TGGAACAAGG
    AAAAGTTTTG ATCACTTGAT ATCAGACACA AAGGCTCCTA AAAGGCAAGA AATGGAATCA
    GGGATCACAA CACCCCCCAA AATGAGGAGA GTAGCAGAAA CTGATTATGA AATGGAAACT
    CAGAGGATTT CCTCATCACA ACAGCACCCA CATTTACGTA AAGTTTCAGT GTCTGAATCA
    AATGTTCTCT TGGATGAAGA AGTACTTACT GATCCGAAGA TCCAGGCGCT GCTTCTTACT
    GTTCTAGCTA CACTGGTAAA ATATACCACA GATGAGTTTG ATCAACGAAT TCTTTATGAA
    TACTTAGCAG AGGCCAGTGT TGTGTTTCCC AAAGTCTTTC CTGTTGTGCA TAATTTGTTG
    GACTCTAAGA TCAACACCCT GTTATCATTG TGCCAAGATC CAAATTTGTT AAATCCAATC
    CATGGAATTG TGCAGAGTGT GGTGTACCAT GAAGAATCCC CACCACAATA CCAAACATCT
    TACCTGCAAA GTTTTGGTTT TAATGGCTTG TGGCGGTTTG CAGGACCGTT TTCAAAGCAA
    ACACAAATTC CAGACTATGC TGAGCTTATT GTTAAGTTTC TTGATGCCTT GATTGACACG
    TACCTGCCTG GAATTGATGA AGAAACCAGT GAAGAATCCC TCCTGACTCC CACATCTCCT
    TACCCTCCTG CACTGCAGAG CCAGCTTAGT ATCACTGCCA ACCTTAACCT TTCTAATTCC
    ATGACCTCAC TTGCAACTTC CCAGCATTCC CCAGGAATCG ACAAGGAGAA CGTTGAACTC
    TCCCCTACCA CTGGCCACTG TAACAGTGGA CGAACTCGCC ACGGATCCGC AAGCCAAGTG
    CAGAAGCAAA GAAGCGCTGG CAGTTTCAAA CGTAATAGCA TTAAGAAGAT CGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50427
    Clone ID Related Accession (Same CDS sequence) XM_006721922.2
    Accession Version XM_006721922.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
    CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
    CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
    AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
    AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
    GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
    CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
    GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
    CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
    GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
    AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
    CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
    TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
    AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
    TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
    AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
    TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
    TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
    ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
    AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
    ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
    ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
    TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
    CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
    TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
    CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
    ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
    ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
    CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
    GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
    TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
    CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
    ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
    CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
    AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
    GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
    CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
    CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
    AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
    TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
    GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
    GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
    GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
    CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
    ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
    CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
    TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
    TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
    TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
    GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
    AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
    GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
    ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
    GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
    CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
    ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
    AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
    CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
    ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
    ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
    CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
    TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
    CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
    TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
    ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
    AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
    AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
    TCCCCAGCTT CTCTGCCTTG CTCTAAATCA GCAGTTTTCA TGCAGCTGTT CCCTCATCAA
    GGAATCGACA AGGAGAACGT TGAACTCTCC CCTACCACTG GCCACTGTAA CAGTGGACGA
    ACTCGCCACG GATCCGCAAG CCAAGTGCAG AAGCAAAGAA GCGCTGGCAG TTTCAAACGT
    AATAGCATTA AGAAGATCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006721985.1
    CDS334..8937
    Translation

    Target ORF information:

    RefSeq Version XM_006721922.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721922.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
    CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
    CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
    AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
    AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
    GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
    CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
    GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
    CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
    GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
    AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
    CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
    TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
    AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
    TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
    AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
    TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
    TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
    ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
    AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
    ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
    ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
    TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
    CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
    TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
    CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
    ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
    ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
    CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
    GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
    TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
    CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
    ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
    CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
    AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
    GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
    CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
    CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
    AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
    TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
    GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
    GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
    GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
    CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
    ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
    CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
    TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
    TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
    TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
    GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
    AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
    GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
    ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
    GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
    CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
    ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
    AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
    CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
    ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
    ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
    CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
    TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
    CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
    TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
    ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
    AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
    AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
    TCCCCAGCTT CTCTGCCTTG CTCTAAATCA GCAGTTTTCA TGCAGCTGTT CCCTCATCAA
    GGAATCGACA AGGAGAACGT TGAACTCTCC CCTACCACTG GCCACTGTAA CAGTGGACGA
    ACTCGCCACG GATCCGCAAG CCAAGTGCAG AAGCAAAGAA GCGCTGGCAG TTTCAAACGT
    AATAGCATTA AGAAGATCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50428
    Clone ID Related Accession (Same CDS sequence) XM_005257983.2
    Accession Version XM_005257983.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurofibromin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257983.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
    CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
    GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
    CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
    GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
    TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
    TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
    GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
    CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
    GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
    AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
    ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
    GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
    ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
    TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
    GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
    ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
    GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
    GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
    GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
    GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
    ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
    GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
    GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
    CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
    GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
    CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
    CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
    GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
    TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
    CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
    CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
    TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
    GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
    CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
    GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
    CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
    CTTCGAAGTG TGTGCCACTG TTTATACCAG GCAACTTGCC ACTCCCTACT GAATAAAGCT
    ACAGTAAAAG AAAAAAAGGA AAACAAAAAA TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC
    AGCATCGGTG CAGTAGGAAG TGCCATGTTC CTCAGATTTA TCAATCCTGC CATTGTCTCA
    CCGTATGAAG CAGGGATTTT AGATAAAAAG CCACCACCTA GAATCGAAAG GGGCTTGAAG
    TTAATGTCAA AGATACTTCA GAGTATTGCC AATCATGTTC TCTTCACAAA AGAAGAACAT
    ATGCGGCCTT TCAATGATTT TGTGAAAAGC AACTTTGATG CAGCACGCAG GTTTTTCCTT
    GATATAGCAT CTGATTGTCC TACAAGTGAT GCAGTAAATC ATAGTCTTTC CTTCATAAGT
    GACGGCAATG TGCTTGCTTT ACATCGTCTA CTCTGGAACA ATCAGGAGAA AATTGGGCAG
    TATCTTTCCA GCAACAGGGA TCATAAAGCT GTTGGAAGAC GACCTTTTGA TAAGATGGCA
    ACACTTCTTG CATACCTGGG TCCTCCAGAG CACAAACCTG TGGCAGATAC ACACTGGTCC
    AGCCTTAACC TTACCAGTTC AAAGTTTGAG GAATTTATGA CTAGGCATCA GGTACATGAA
    AAAGAAGAAT TCAAGGCTTT GAAAACGTTA AGTATTTTCT ACCAAGCTGG GACTTCCAAA
    GCTGGGAATC CTATTTTTTA TTATGTTGCA CGGAGGTTCA AAACTGGTCA AATCAATGGT
    GATTTGCTGA TATACCATGT CTTACTGACT TTAAAGCCAT ATTATGCAAA GCCATATGAA
    ATTGTAGTGG ACCTTACCCA TACCGGGCCT AGCAATCGCT TTAAAACAGA CTTTCTCTCT
    AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT TACGACAACG TCTCCGCAGT CTATATCTAT
    AACTGTAACT CCTGGGTCAG GGAGTACACC AAGTATCATG AGCGGCTGCT GACTGGCCTC
    AAAGGTAGCA AAAGGCTTGT TTTCATAGAC TGTCCTGGGA AACTGGCTGA GCACATAGAG
    CATGAACAAC AGAAACTACC TGCTGCCACC TTGGCTTTAG AAGAGGACCT GAAGGTATTC
    CACAATGCTC TCAAGCTAGC TCACAAAGAC ACCAAAGTTT CTATTAAAGT TGGTTCTACT
    GCTGTCCAAG TAACTTCAGC AGAGCGAACA AAAGTCCTAG GGCAATCAGT CTTTCTAAAT
    GACATTTATT ATGCTTCGGA AATTGAAGAA ATCTGCCTAG TAGATGAGAA CCAGTTCACC
    TTAACCATTG CAAACCAGGG CACGCCGCTC ACCTTCATGC ACCAGGAGTG TGAAGCCATT
    GTCCAGTCTA TCATTCATAT CCGGACCCGC TGGGAACTGT CACAGCCCGA CTCTATCCCC
    CAACACACCA AGATTCGGCC AAAAGATGTC CCTGGGACAC TGCTCAATAT CGCATTACTT
    AATTTAGGCA GTTCTGACCC GAGTTTACGG TCAGCTGCCT ATAATCTTCT GTGTGCCTTA
    ACTTGTACCT TTAATTTAAA AATCGAGGGC CAGTTACTAG AGACATCAGG TTTATGTATC
    CCTGCCAACA ACACCCTCTT TATTGTCTCT ATTAGTAAGA CACTGGCAGC CAATGAGCCA
    CACCTCACGT TAGAATTTTT GGAAGAGTGT ATTTCTGGAT TTAGCAAATC TAGTATTGAA
    TTGAAACACC TTTGTTTGGA ATACATGACT CCATGGCTGT CAAATCTAGT TCGTTTTTGC
    AAGCATAATG ATGATGCCAA ACGACAAAGA GTTACTGCTA TTCTTGACAA GCTGATAACA
    ATGACCATCA ATGAAAAACA GATGTACCCA TCTATTCAAG CAAAAATATG GGGAAGCCTT
    GGGCAGATTA CAGATCTGCT TGATGTTGTA CTAGACAGTT TCATCAAAAC CAGTGCAACA
    GGTGGCTTGG GATCAATAAA AGCTGAGGTG ATGGCAGATA CTGCTGTAGC TTTGGCTTCT
    GGAAATGTGA AATTGGTTTC AAGCAAGGTT ATTGGAAGGA TGTGCAAAAT AATTGACAAG
    ACATGCTTAT CTCCAACTCC TACTTTAGAA CAACATCTTA TGTGGGATGA TATTGCTATT
    TTAGCACGCT ACATGCTGAT GCTGTCCTTC AACAATTCCC TTGATGTGGC AGCTCATCTT
    CCCTACCTCT TCCACGTTGT TACTTTCTTA GTAGCCACAG GTCCGCTCTC CCTTAGAGCT
    TCCACACATG GACTGGTCAT TAATATCATT CACTCTCTGT GTACTTGTTC ACAGCTTCAT
    TTTAGTGAAG AGACCAAGCA AGTTTTGAGA CTCAGTCTGA CAGAGTTCTC ATTACCCAAA
    TTTTACTTGC TGTTTGGCAT TAGCAAAGTC AAGTCAGCTG CTGTCATTGC CTTCCGTTCC
    AGTTACCGGG ACAGGTCATT CTCTCCTGGC TCCTATGAGA GAGAGACTTT TGCTTTGACA
    TCCTTGGAAA CAGTCACAGA AGCTTTGTTG GAGATCATGG AGGCATGCAT GAGAGATATT
    CCAACGTGCA AGTGGCTGGA CCAGTGGACA GAACTAGCTC AAAGATTTGC ATTCCAATAT
    AATCCATCCC TGCAACCAAG AGCTCTTGTT GTCTTTGGGT GTATTAGCAA ACGAGTGTCT
    CATGGGCAGA TAAAGCAGAT AATCCGTATT CTTAGCAAGG CACTTGAGAG TTGCTTAAAA
    GGACCTGACA CTTACAACAG TCAAGTTCTG ATAGAAGCTA CAGTAATAGC ACTAACCAAA
    TTACAGCCAC TTCTTAATAA GGACTCGCCT CTGCACAAAG CCCTCTTTTG GGTAGCTGTG
    GCTGTGCTGC AGCTTGATGA GGTCAACTTG TATTCAGCAG GTACCGCACT TCTTGAACAA
    AACCTGCATA CTTTAGATAG TCTCCGTATA TTCAATGACA AGAGTCCAGA GGAAGTATTT
    ATGGCAATCC GGAATCCTCT GGAGTGGCAC TGCAAGCAAA TGGATCATTT TGTTGGACTC
    AATTTCAACT CTAACTTTAA CTTTGCATTG GTTGGACACC TTTTAAAAGG GTACAGGCAT
    CCTTCACCTG CTATTGTTGC AAGAACAGTC AGAATTTTAC ATACACTACT AACTCTGGTT
    AACAAACACA GAAATTGTGA CAAATTTGAA GTGAATACAC AGAGCGTGGC CTACTTAGCA
    GCTTTACTTA CAGTGTCTGA AGAAGTTCGA AGTCGCTGCA GCCTAAAACA TAGAAAGTCA
    CTTCTTCTTA CTGATATTTC AATGGAAAAT GTTCCTATGG ATACATATCC CATTCATCAT
    GGTGACCCTT CCTATAGGAC ACTAAAGGAG ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT
    GAAGGATACC TTGCAGCCAC CTATCCAACT GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA
    TCCATGAGCC TGGACATGGG GCAACCTTCT CAGGCCAACA CTAAGAAGTT GCTTGGAACA
    AGGAAAAGTT TTGATCACTT GATATCAGAC ACAAAGGCTC CTAAAAGGCA AGAAATGGAA
    TCAGGGATCA CAACACCCCC CAAAATGAGG AGAGTAGCAG AAACTGATTA TGAAATGGAA
    ACTCAGAGGA TTTCCTCATC ACAACAGCAC CCACATTTAC GTAAAGTTTC AGTGTCTGAA
    TCAAATGTTC TCTTGGATGA AGAAGTACTT ACTGATCCGA AGATCCAGGC GCTGCTTCTT
    ACTGTTCTAG CTACACTGGT AAAATATACC ACAGATGAGT TTGATCAACG AATTCTTTAT
    GAATACTTAG CAGAGGCCAG TGTTGTGTTT CCCAAAGTCT TTCCTGTTGT GCATAATTTG
    TTGGACTCTA AGATCAACAC CCTGTTATCA TTGTGCCAAG ATCCAAATTT GTTAAATCCA
    ATCCATGGAA TTGTGCAGAG TGTGGTGTAC CATGAAGAAT CCCCACCACA ATACCAAACA
    TCTTACCTGC AAAGTTTTGG TTTTAATGGC TTGTGGCGGT TTGCAGGACC GTTTTCAAAG
    CAAACACAAA TTCCAGACTA TGCTGAGCTT ATTGTTAAGT TTCTTGATGC CTTGATTGAC
    ACGTACCTGC CTGGAATTGA TGAAGAAACC AGTGAAGAAT CCCTCCTGAC TCCCACATCT
    CCTTACCCTC CTGCACTGCA GAGCCAGCTT AGTATCACTG CCAACCTTAA CCTTTCTAAT
    TCCATGACCT CACTTGCAAC TTCCCAGCAT TCCCCAGCTT CTCTGCCTTG CTCTAAATCA
    GCAGTTTTCA TGCAGCTGTT CCCTCATCAA GGAATCGACA AGGAGAACGT TGAACTCTCC
    CCTACCACTG GCCACTGTAA CAGTGGACGA ACTCGCCACG GATCCGCAAG CCAAGTGCAG
    AAGCAAAGAA GCGCTGGCAG TTTCAAACGT AATAGCATTA AGAAGATCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005258040.1
    CDS334..8907
    Translation

    Target ORF information:

    RefSeq Version XM_005257983.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005257983.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
    CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
    GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
    CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
    GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
    TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
    TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
    GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
    CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
    GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
    AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
    ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
    GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
    ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
    TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
    GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
    ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
    GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
    GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
    GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
    GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
    ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
    GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
    GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
    CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
    GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
    CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
    CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
    GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
    TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
    CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
    CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
    TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
    GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
    CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
    GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
    CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
    CTTCGAAGTG TGTGCCACTG TTTATACCAG GCAACTTGCC ACTCCCTACT GAATAAAGCT
    ACAGTAAAAG AAAAAAAGGA AAACAAAAAA TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC
    AGCATCGGTG CAGTAGGAAG TGCCATGTTC CTCAGATTTA TCAATCCTGC CATTGTCTCA
    CCGTATGAAG CAGGGATTTT AGATAAAAAG CCACCACCTA GAATCGAAAG GGGCTTGAAG
    TTAATGTCAA AGATACTTCA GAGTATTGCC AATCATGTTC TCTTCACAAA AGAAGAACAT
    ATGCGGCCTT TCAATGATTT TGTGAAAAGC AACTTTGATG CAGCACGCAG GTTTTTCCTT
    GATATAGCAT CTGATTGTCC TACAAGTGAT GCAGTAAATC ATAGTCTTTC CTTCATAAGT
    GACGGCAATG TGCTTGCTTT ACATCGTCTA CTCTGGAACA ATCAGGAGAA AATTGGGCAG
    TATCTTTCCA GCAACAGGGA TCATAAAGCT GTTGGAAGAC GACCTTTTGA TAAGATGGCA
    ACACTTCTTG CATACCTGGG TCCTCCAGAG CACAAACCTG TGGCAGATAC ACACTGGTCC
    AGCCTTAACC TTACCAGTTC AAAGTTTGAG GAATTTATGA CTAGGCATCA GGTACATGAA
    AAAGAAGAAT TCAAGGCTTT GAAAACGTTA AGTATTTTCT ACCAAGCTGG GACTTCCAAA
    GCTGGGAATC CTATTTTTTA TTATGTTGCA CGGAGGTTCA AAACTGGTCA AATCAATGGT
    GATTTGCTGA TATACCATGT CTTACTGACT TTAAAGCCAT ATTATGCAAA GCCATATGAA
    ATTGTAGTGG ACCTTACCCA TACCGGGCCT AGCAATCGCT TTAAAACAGA CTTTCTCTCT
    AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT TACGACAACG TCTCCGCAGT CTATATCTAT
    AACTGTAACT CCTGGGTCAG GGAGTACACC AAGTATCATG AGCGGCTGCT GACTGGCCTC
    AAAGGTAGCA AAAGGCTTGT TTTCATAGAC TGTCCTGGGA AACTGGCTGA GCACATAGAG
    CATGAACAAC AGAAACTACC TGCTGCCACC TTGGCTTTAG AAGAGGACCT GAAGGTATTC
    CACAATGCTC TCAAGCTAGC TCACAAAGAC ACCAAAGTTT CTATTAAAGT TGGTTCTACT
    GCTGTCCAAG TAACTTCAGC AGAGCGAACA AAAGTCCTAG GGCAATCAGT CTTTCTAAAT
    GACATTTATT ATGCTTCGGA AATTGAAGAA ATCTGCCTAG TAGATGAGAA CCAGTTCACC
    TTAACCATTG CAAACCAGGG CACGCCGCTC ACCTTCATGC ACCAGGAGTG TGAAGCCATT
    GTCCAGTCTA TCATTCATAT CCGGACCCGC TGGGAACTGT CACAGCCCGA CTCTATCCCC
    CAACACACCA AGATTCGGCC AAAAGATGTC CCTGGGACAC TGCTCAATAT CGCATTACTT
    AATTTAGGCA GTTCTGACCC GAGTTTACGG TCAGCTGCCT ATAATCTTCT GTGTGCCTTA
    ACTTGTACCT TTAATTTAAA AATCGAGGGC CAGTTACTAG AGACATCAGG TTTATGTATC
    CCTGCCAACA ACACCCTCTT TATTGTCTCT ATTAGTAAGA CACTGGCAGC CAATGAGCCA
    CACCTCACGT TAGAATTTTT GGAAGAGTGT ATTTCTGGAT TTAGCAAATC TAGTATTGAA
    TTGAAACACC TTTGTTTGGA ATACATGACT CCATGGCTGT CAAATCTAGT TCGTTTTTGC
    AAGCATAATG ATGATGCCAA ACGACAAAGA GTTACTGCTA TTCTTGACAA GCTGATAACA
    ATGACCATCA ATGAAAAACA GATGTACCCA TCTATTCAAG CAAAAATATG GGGAAGCCTT
    GGGCAGATTA CAGATCTGCT TGATGTTGTA CTAGACAGTT TCATCAAAAC CAGTGCAACA
    GGTGGCTTGG GATCAATAAA AGCTGAGGTG ATGGCAGATA CTGCTGTAGC TTTGGCTTCT
    GGAAATGTGA AATTGGTTTC AAGCAAGGTT ATTGGAAGGA TGTGCAAAAT AATTGACAAG
    ACATGCTTAT CTCCAACTCC TACTTTAGAA CAACATCTTA TGTGGGATGA TATTGCTATT
    TTAGCACGCT ACATGCTGAT GCTGTCCTTC AACAATTCCC TTGATGTGGC AGCTCATCTT
    CCCTACCTCT TCCACGTTGT TACTTTCTTA GTAGCCACAG GTCCGCTCTC CCTTAGAGCT
    TCCACACATG GACTGGTCAT TAATATCATT CACTCTCTGT GTACTTGTTC ACAGCTTCAT
    TTTAGTGAAG AGACCAAGCA AGTTTTGAGA CTCAGTCTGA CAGAGTTCTC ATTACCCAAA
    TTTTACTTGC TGTTTGGCAT TAGCAAAGTC AAGTCAGCTG CTGTCATTGC CTTCCGTTCC
    AGTTACCGGG ACAGGTCATT CTCTCCTGGC TCCTATGAGA GAGAGACTTT TGCTTTGACA
    TCCTTGGAAA CAGTCACAGA AGCTTTGTTG GAGATCATGG AGGCATGCAT GAGAGATATT
    CCAACGTGCA AGTGGCTGGA CCAGTGGACA GAACTAGCTC AAAGATTTGC ATTCCAATAT
    AATCCATCCC TGCAACCAAG AGCTCTTGTT GTCTTTGGGT GTATTAGCAA ACGAGTGTCT
    CATGGGCAGA TAAAGCAGAT AATCCGTATT CTTAGCAAGG CACTTGAGAG TTGCTTAAAA
    GGACCTGACA CTTACAACAG TCAAGTTCTG ATAGAAGCTA CAGTAATAGC ACTAACCAAA
    TTACAGCCAC TTCTTAATAA GGACTCGCCT CTGCACAAAG CCCTCTTTTG GGTAGCTGTG
    GCTGTGCTGC AGCTTGATGA GGTCAACTTG TATTCAGCAG GTACCGCACT TCTTGAACAA
    AACCTGCATA CTTTAGATAG TCTCCGTATA TTCAATGACA AGAGTCCAGA GGAAGTATTT
    ATGGCAATCC GGAATCCTCT GGAGTGGCAC TGCAAGCAAA TGGATCATTT TGTTGGACTC
    AATTTCAACT CTAACTTTAA CTTTGCATTG GTTGGACACC TTTTAAAAGG GTACAGGCAT
    CCTTCACCTG CTATTGTTGC AAGAACAGTC AGAATTTTAC ATACACTACT AACTCTGGTT
    AACAAACACA GAAATTGTGA CAAATTTGAA GTGAATACAC AGAGCGTGGC CTACTTAGCA
    GCTTTACTTA CAGTGTCTGA AGAAGTTCGA AGTCGCTGCA GCCTAAAACA TAGAAAGTCA
    CTTCTTCTTA CTGATATTTC AATGGAAAAT GTTCCTATGG ATACATATCC CATTCATCAT
    GGTGACCCTT CCTATAGGAC ACTAAAGGAG ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT
    GAAGGATACC TTGCAGCCAC CTATCCAACT GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA
    TCCATGAGCC TGGACATGGG GCAACCTTCT CAGGCCAACA CTAAGAAGTT GCTTGGAACA
    AGGAAAAGTT TTGATCACTT GATATCAGAC ACAAAGGCTC CTAAAAGGCA AGAAATGGAA
    TCAGGGATCA CAACACCCCC CAAAATGAGG AGAGTAGCAG AAACTGATTA TGAAATGGAA
    ACTCAGAGGA TTTCCTCATC ACAACAGCAC CCACATTTAC GTAAAGTTTC AGTGTCTGAA
    TCAAATGTTC TCTTGGATGA AGAAGTACTT ACTGATCCGA AGATCCAGGC GCTGCTTCTT
    ACTGTTCTAG CTACACTGGT AAAATATACC ACAGATGAGT TTGATCAACG AATTCTTTAT
    GAATACTTAG CAGAGGCCAG TGTTGTGTTT CCCAAAGTCT TTCCTGTTGT GCATAATTTG
    TTGGACTCTA AGATCAACAC CCTGTTATCA TTGTGCCAAG ATCCAAATTT GTTAAATCCA
    ATCCATGGAA TTGTGCAGAG TGTGGTGTAC CATGAAGAAT CCCCACCACA ATACCAAACA
    TCTTACCTGC AAAGTTTTGG TTTTAATGGC TTGTGGCGGT TTGCAGGACC GTTTTCAAAG
    CAAACACAAA TTCCAGACTA TGCTGAGCTT ATTGTTAAGT TTCTTGATGC CTTGATTGAC
    ACGTACCTGC CTGGAATTGA TGAAGAAACC AGTGAAGAAT CCCTCCTGAC TCCCACATCT
    CCTTACCCTC CTGCACTGCA GAGCCAGCTT AGTATCACTG CCAACCTTAA CCTTTCTAAT
    TCCATGACCT CACTTGCAAC TTCCCAGCAT TCCCCAGCTT CTCTGCCTTG CTCTAAATCA
    GCAGTTTTCA TGCAGCTGTT CCCTCATCAA GGAATCGACA AGGAGAACGT TGAACTCTCC
    CCTACCACTG GCCACTGTAA CAGTGGACGA ACTCGCCACG GATCCGCAAG CCAAGTGCAG
    AAGCAAAGAA GCGCTGGCAG TTTCAAACGT AATAGCATTA AGAAGATCGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50430
    Clone ID Related Accession (Same CDS sequence) XM_006721924.2
    Accession Version XM_006721924.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurofibromin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721924.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1621
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    1801
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    8401
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    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
    CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
    CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
    AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
    AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
    GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
    CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
    GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
    CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
    GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
    AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
    CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
    TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
    AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
    TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
    AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
    TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
    TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
    ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
    AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
    ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
    ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
    TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
    CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
    TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
    CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
    ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
    ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
    CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
    GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
    TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
    CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
    ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
    CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
    AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
    GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
    CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
    CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
    AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
    TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
    GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
    GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
    GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
    CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
    ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
    CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
    TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
    TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
    TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
    GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
    AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
    GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
    ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
    GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
    CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
    ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
    AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
    CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
    ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
    ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
    CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
    TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
    CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
    TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
    ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
    AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
    AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
    TCCCCAGGAA TCGACAAGGA GAACGTTGAA CTCTCCCCTA CCACTGGCCA CTGTAACAGT
    GGACGAACTC GCCACGGATC CGCAAGCCAA GTGCAGAAGC AAAGAAGCGC TGGCAGTTTC
    AAACGTAATA GCATTAAGAA GATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006721987.1
    CDS334..8883
    Translation

    Target ORF information:

    RefSeq Version XM_006721924.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721924.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
    CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
    CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
    AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
    AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
    GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
    CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
    GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
    CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
    GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
    AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
    CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
    TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
    AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
    TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
    AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
    TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
    TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
    ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
    AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
    ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
    ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
    TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
    CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
    TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
    CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
    ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
    ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
    CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
    GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
    TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
    CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
    ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
    CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
    AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
    GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
    CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
    CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
    AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
    TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
    GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
    GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
    GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
    CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
    ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
    CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
    TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
    TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
    TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
    GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
    AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
    GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
    ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
    GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
    CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
    ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
    AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
    CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
    ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
    ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
    CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
    TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
    CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
    TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
    ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
    AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
    AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
    TCCCCAGGAA TCGACAAGGA GAACGTTGAA CTCTCCCCTA CCACTGGCCA CTGTAACAGT
    GGACGAACTC GCCACGGATC CGCAAGCCAA GTGCAGAAGC AAAGAAGCGC TGGCAGTTTC
    AAACGTAATA GCATTAAGAA GATCGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50431
    Clone ID Related Accession (Same CDS sequence) XM_006721925.2
    Accession Version XM_006721925.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8541bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurofibromin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721925.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    GTGGTTAGCC AGCGTTTCCC TCAGAACAGC ATCGGTGCAG TAGGAAGTGC CATGTTCCTC
    AGATTTATCA ATCCTGCCAT TGTCTCACCG TATGAAGCAG GGATTTTAGA TAAAAAGCCA
    CCACCTAGAA TCGAAAGGGG CTTGAAGTTA ATGTCAAAGA TACTTCAGAG TATTGCCAAT
    CATGTTCTCT TCACAAAAGA AGAACATATG CGGCCTTTCA ATGATTTTGT GAAAAGCAAC
    TTTGATGCAG CACGCAGGTT TTTCCTTGAT ATAGCATCTG ATTGTCCTAC AAGTGATGCA
    GTAAATCATA GTCTTTCCTT CATAAGTGAC GGCAATGTGC TTGCTTTACA TCGTCTACTC
    TGGAACAATC AGGAGAAAAT TGGGCAGTAT CTTTCCAGCA ACAGGGATCA TAAAGCTGTT
    GGAAGACGAC CTTTTGATAA GATGGCAACA CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC
    AAACCTGTGG CAGATACACA CTGGTCCAGC CTTAACCTTA CCAGTTCAAA GTTTGAGGAA
    TTTATGACTA GGCATCAGGT ACATGAAAAA GAAGAATTCA AGGCTTTGAA AACGTTAAGT
    ATTTTCTACC AAGCTGGGAC TTCCAAAGCT GGGAATCCTA TTTTTTATTA TGTTGCACGG
    AGGTTCAAAA CTGGTCAAAT CAATGGTGAT TTGCTGATAT ACCATGTCTT ACTGACTTTA
    AAGCCATATT ATGCAAAGCC ATATGAAATT GTAGTGGACC TTACCCATAC CGGGCCTAGC
    AATCGCTTTA AAACAGACTT TCTCTCTAAG TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC
    GACAACGTCT CCGCAGTCTA TATCTATAAC TGTAACTCCT GGGTCAGGGA GTACACCAAG
    TATCATGAGC GGCTGCTGAC TGGCCTCAAA GGTAGCAAAA GGCTTGTTTT CATAGACTGT
    CCTGGGAAAC TGGCTGAGCA CATAGAGCAT GAACAACAGA AACTACCTGC TGCCACCTTG
    GCTTTAGAAG AGGACCTGAA GGTATTCCAC AATGCTCTCA AGCTAGCTCA CAAAGACACC
    AAAGTTTCTA TTAAAGTTGG TTCTACTGCT GTCCAAGTAA CTTCAGCAGA GCGAACAAAA
    GTCCTAGGGC AATCAGTCTT TCTAAATGAC ATTTATTATG CTTCGGAAAT TGAAGAAATC
    TGCCTAGTAG ATGAGAACCA GTTCACCTTA ACCATTGCAA ACCAGGGCAC GCCGCTCACC
    TTCATGCACC AGGAGTGTGA AGCCATTGTC CAGTCTATCA TTCATATCCG GACCCGCTGG
    GAACTGTCAC AGCCCGACTC TATCCCCCAA CACACCAAGA TTCGGCCAAA AGATGTCCCT
    GGGACACTGC TCAATATCGC ATTACTTAAT TTAGGCAGTT CTGACCCGAG TTTACGGTCA
    GCTGCCTATA ATCTTCTGTG TGCCTTAACT TGTACCTTTA ATTTAAAAAT CGAGGGCCAG
    TTACTAGAGA CATCAGGTTT ATGTATCCCT GCCAACAACA CCCTCTTTAT TGTCTCTATT
    AGTAAGACAC TGGCAGCCAA TGAGCCACAC CTCACGTTAG AATTTTTGGA AGAGTGTATT
    TCTGGATTTA GCAAATCTAG TATTGAATTG AAACACCTTT GTTTGGAATA CATGACTCCA
    TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG CATAATGATG ATGCCAAACG ACAAAGAGTT
    ACTGCTATTC TTGACAAGCT GATAACAATG ACCATCAATG AAAAACAGAT GTACCCATCT
    ATTCAAGCAA AAATATGGGG AAGCCTTGGG CAGATTACAG ATCTGCTTGA TGTTGTACTA
    GACAGTTTCA TCAAAACCAG TGCAACAGGT GGCTTGGGAT CAATAAAAGC TGAGGTGATG
    GCAGATACTG CTGTAGCTTT GGCTTCTGGA AATGTGAAAT TGGTTTCAAG CAAGGTTATT
    GGAAGGATGT GCAAAATAAT TGACAAGACA TGCTTATCTC CAACTCCTAC TTTAGAACAA
    CATCTTATGT GGGATGATAT TGCTATTTTA GCACGCTACA TGCTGATGCT GTCCTTCAAC
    AATTCCCTTG ATGTGGCAGC TCATCTTCCC TACCTCTTCC ACGTTGTTAC TTTCTTAGTA
    GCCACAGGTC CGCTCTCCCT TAGAGCTTCC ACACATGGAC TGGTCATTAA TATCATTCAC
    TCTCTGTGTA CTTGTTCACA GCTTCATTTT AGTGAAGAGA CCAAGCAAGT TTTGAGACTC
    AGTCTGACAG AGTTCTCATT ACCCAAATTT TACTTGCTGT TTGGCATTAG CAAAGTCAAG
    TCAGCTGCTG TCATTGCCTT CCGTTCCAGT TACCGGGACA GGTCATTCTC TCCTGGCTCC
    TATGAGAGAG AGACTTTTGC TTTGACATCC TTGGAAACAG TCACAGAAGC TTTGTTGGAG
    ATCATGGAGG CATGCATGAG AGATATTCCA ACGTGCAAGT GGCTGGACCA GTGGACAGAA
    CTAGCTCAAA GATTTGCATT CCAATATAAT CCATCCCTGC AACCAAGAGC TCTTGTTGTC
    TTTGGGTGTA TTAGCAAACG AGTGTCTCAT GGGCAGATAA AGCAGATAAT CCGTATTCTT
    AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA CCTGACACTT ACAACAGTCA AGTTCTGATA
    GAAGCTACAG TAATAGCACT AACCAAATTA CAGCCACTTC TTAATAAGGA CTCGCCTCTG
    CACAAAGCCC TCTTTTGGGT AGCTGTGGCT GTGCTGCAGC TTGATGAGGT CAACTTGTAT
    TCAGCAGGTA CCGCACTTCT TGAACAAAAC CTGCATACTT TAGATAGTCT CCGTATATTC
    AATGACAAGA GTCCAGAGGA AGTATTTATG GCAATCCGGA ATCCTCTGGA GTGGCACTGC
    AAGCAAATGG ATCATTTTGT TGGACTCAAT TTCAACTCTA ACTTTAACTT TGCATTGGTT
    GGACACCTTT TAAAAGGGTA CAGGCATCCT TCACCTGCTA TTGTTGCAAG AACAGTCAGA
    ATTTTACATA CACTACTAAC TCTGGTTAAC AAACACAGAA ATTGTGACAA ATTTGAAGTG
    AATACACAGA GCGTGGCCTA CTTAGCAGCT TTACTTACAG TGTCTGAAGA AGTTCGAAGT
    CGCTGCAGCC TAAAACATAG AAAGTCACTT CTTCTTACTG ATATTTCAAT GGAAAATGTT
    CCTATGGATA CATATCCCAT TCATCATGGT GACCCTTCCT ATAGGACACT AAAGGAGACT
    CAGCCATGGT CCTCTCCCAA AGGTTCTGAA GGATACCTTG CAGCCACCTA TCCAACTGTC
    GGCCAGACCA GTCCCCGAGC CAGGAAATCC ATGAGCCTGG ACATGGGGCA ACCTTCTCAG
    GCCAACACTA AGAAGTTGCT TGGAACAAGG AAAAGTTTTG ATCACTTGAT ATCAGACACA
    AAGGCTCCTA AAAGGCAAGA AATGGAATCA GGGATCACAA CACCCCCCAA AATGAGGAGA
    GTAGCAGAAA CTGATTATGA AATGGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
    CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
    GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
    GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
    AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
    TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
    GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
    TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
    GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
    GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
    ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
    CCAGCTTCTC TGCCTTGCTC TAAATCAGCA GTTTTCATGC AGCTGTTCCC TCATCAAGGA
    ATCGACAAGG AGAACGTTGA ACTCTCCCCT ACCACTGGCC ACTGTAACAG TGGACGAACT
    CGCCACGGAT CCGCAAGCCA AGTGCAGAAG CAAAGAAGCG CTGGCAGTTT CAAACGTAAT
    AGCATTAAGA AGATCGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006721988.1
    CDS334..8874
    Translation

    Target ORF information:

    RefSeq Version XM_006721925.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721925.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    GTGGTTAGCC AGCGTTTCCC TCAGAACAGC ATCGGTGCAG TAGGAAGTGC CATGTTCCTC
    AGATTTATCA ATCCTGCCAT TGTCTCACCG TATGAAGCAG GGATTTTAGA TAAAAAGCCA
    CCACCTAGAA TCGAAAGGGG CTTGAAGTTA ATGTCAAAGA TACTTCAGAG TATTGCCAAT
    CATGTTCTCT TCACAAAAGA AGAACATATG CGGCCTTTCA ATGATTTTGT GAAAAGCAAC
    TTTGATGCAG CACGCAGGTT TTTCCTTGAT ATAGCATCTG ATTGTCCTAC AAGTGATGCA
    GTAAATCATA GTCTTTCCTT CATAAGTGAC GGCAATGTGC TTGCTTTACA TCGTCTACTC
    TGGAACAATC AGGAGAAAAT TGGGCAGTAT CTTTCCAGCA ACAGGGATCA TAAAGCTGTT
    GGAAGACGAC CTTTTGATAA GATGGCAACA CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC
    AAACCTGTGG CAGATACACA CTGGTCCAGC CTTAACCTTA CCAGTTCAAA GTTTGAGGAA
    TTTATGACTA GGCATCAGGT ACATGAAAAA GAAGAATTCA AGGCTTTGAA AACGTTAAGT
    ATTTTCTACC AAGCTGGGAC TTCCAAAGCT GGGAATCCTA TTTTTTATTA TGTTGCACGG
    AGGTTCAAAA CTGGTCAAAT CAATGGTGAT TTGCTGATAT ACCATGTCTT ACTGACTTTA
    AAGCCATATT ATGCAAAGCC ATATGAAATT GTAGTGGACC TTACCCATAC CGGGCCTAGC
    AATCGCTTTA AAACAGACTT TCTCTCTAAG TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC
    GACAACGTCT CCGCAGTCTA TATCTATAAC TGTAACTCCT GGGTCAGGGA GTACACCAAG
    TATCATGAGC GGCTGCTGAC TGGCCTCAAA GGTAGCAAAA GGCTTGTTTT CATAGACTGT
    CCTGGGAAAC TGGCTGAGCA CATAGAGCAT GAACAACAGA AACTACCTGC TGCCACCTTG
    GCTTTAGAAG AGGACCTGAA GGTATTCCAC AATGCTCTCA AGCTAGCTCA CAAAGACACC
    AAAGTTTCTA TTAAAGTTGG TTCTACTGCT GTCCAAGTAA CTTCAGCAGA GCGAACAAAA
    GTCCTAGGGC AATCAGTCTT TCTAAATGAC ATTTATTATG CTTCGGAAAT TGAAGAAATC
    TGCCTAGTAG ATGAGAACCA GTTCACCTTA ACCATTGCAA ACCAGGGCAC GCCGCTCACC
    TTCATGCACC AGGAGTGTGA AGCCATTGTC CAGTCTATCA TTCATATCCG GACCCGCTGG
    GAACTGTCAC AGCCCGACTC TATCCCCCAA CACACCAAGA TTCGGCCAAA AGATGTCCCT
    GGGACACTGC TCAATATCGC ATTACTTAAT TTAGGCAGTT CTGACCCGAG TTTACGGTCA
    GCTGCCTATA ATCTTCTGTG TGCCTTAACT TGTACCTTTA ATTTAAAAAT CGAGGGCCAG
    TTACTAGAGA CATCAGGTTT ATGTATCCCT GCCAACAACA CCCTCTTTAT TGTCTCTATT
    AGTAAGACAC TGGCAGCCAA TGAGCCACAC CTCACGTTAG AATTTTTGGA AGAGTGTATT
    TCTGGATTTA GCAAATCTAG TATTGAATTG AAACACCTTT GTTTGGAATA CATGACTCCA
    TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG CATAATGATG ATGCCAAACG ACAAAGAGTT
    ACTGCTATTC TTGACAAGCT GATAACAATG ACCATCAATG AAAAACAGAT GTACCCATCT
    ATTCAAGCAA AAATATGGGG AAGCCTTGGG CAGATTACAG ATCTGCTTGA TGTTGTACTA
    GACAGTTTCA TCAAAACCAG TGCAACAGGT GGCTTGGGAT CAATAAAAGC TGAGGTGATG
    GCAGATACTG CTGTAGCTTT GGCTTCTGGA AATGTGAAAT TGGTTTCAAG CAAGGTTATT
    GGAAGGATGT GCAAAATAAT TGACAAGACA TGCTTATCTC CAACTCCTAC TTTAGAACAA
    CATCTTATGT GGGATGATAT TGCTATTTTA GCACGCTACA TGCTGATGCT GTCCTTCAAC
    AATTCCCTTG ATGTGGCAGC TCATCTTCCC TACCTCTTCC ACGTTGTTAC TTTCTTAGTA
    GCCACAGGTC CGCTCTCCCT TAGAGCTTCC ACACATGGAC TGGTCATTAA TATCATTCAC
    TCTCTGTGTA CTTGTTCACA GCTTCATTTT AGTGAAGAGA CCAAGCAAGT TTTGAGACTC
    AGTCTGACAG AGTTCTCATT ACCCAAATTT TACTTGCTGT TTGGCATTAG CAAAGTCAAG
    TCAGCTGCTG TCATTGCCTT CCGTTCCAGT TACCGGGACA GGTCATTCTC TCCTGGCTCC
    TATGAGAGAG AGACTTTTGC TTTGACATCC TTGGAAACAG TCACAGAAGC TTTGTTGGAG
    ATCATGGAGG CATGCATGAG AGATATTCCA ACGTGCAAGT GGCTGGACCA GTGGACAGAA
    CTAGCTCAAA GATTTGCATT CCAATATAAT CCATCCCTGC AACCAAGAGC TCTTGTTGTC
    TTTGGGTGTA TTAGCAAACG AGTGTCTCAT GGGCAGATAA AGCAGATAAT CCGTATTCTT
    AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA CCTGACACTT ACAACAGTCA AGTTCTGATA
    GAAGCTACAG TAATAGCACT AACCAAATTA CAGCCACTTC TTAATAAGGA CTCGCCTCTG
    CACAAAGCCC TCTTTTGGGT AGCTGTGGCT GTGCTGCAGC TTGATGAGGT CAACTTGTAT
    TCAGCAGGTA CCGCACTTCT TGAACAAAAC CTGCATACTT TAGATAGTCT CCGTATATTC
    AATGACAAGA GTCCAGAGGA AGTATTTATG GCAATCCGGA ATCCTCTGGA GTGGCACTGC
    AAGCAAATGG ATCATTTTGT TGGACTCAAT TTCAACTCTA ACTTTAACTT TGCATTGGTT
    GGACACCTTT TAAAAGGGTA CAGGCATCCT TCACCTGCTA TTGTTGCAAG AACAGTCAGA
    ATTTTACATA CACTACTAAC TCTGGTTAAC AAACACAGAA ATTGTGACAA ATTTGAAGTG
    AATACACAGA GCGTGGCCTA CTTAGCAGCT TTACTTACAG TGTCTGAAGA AGTTCGAAGT
    CGCTGCAGCC TAAAACATAG AAAGTCACTT CTTCTTACTG ATATTTCAAT GGAAAATGTT
    CCTATGGATA CATATCCCAT TCATCATGGT GACCCTTCCT ATAGGACACT AAAGGAGACT
    CAGCCATGGT CCTCTCCCAA AGGTTCTGAA GGATACCTTG CAGCCACCTA TCCAACTGTC
    GGCCAGACCA GTCCCCGAGC CAGGAAATCC ATGAGCCTGG ACATGGGGCA ACCTTCTCAG
    GCCAACACTA AGAAGTTGCT TGGAACAAGG AAAAGTTTTG ATCACTTGAT ATCAGACACA
    AAGGCTCCTA AAAGGCAAGA AATGGAATCA GGGATCACAA CACCCCCCAA AATGAGGAGA
    GTAGCAGAAA CTGATTATGA AATGGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
    CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
    GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
    GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
    AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
    TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
    GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
    TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
    GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
    GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
    ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
    CCAGCTTCTC TGCCTTGCTC TAAATCAGCA GTTTTCATGC AGCTGTTCCC TCATCAAGGA
    ATCGACAAGG AGAACGTTGA ACTCTCCCCT ACCACTGGCC ACTGTAACAG TGGACGAACT
    CGCCACGGAT CCGCAAGCCA AGTGCAGAAG CAAAGAAGCG CTGGCAGTTT CAAACGTAAT
    AGCATTAAGA AGATCGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50432
    Clone ID Related Accession (Same CDS sequence) XM_005257984.2
    Accession Version XM_005257984.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurofibromin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
    CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
    GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
    CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
    GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
    TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
    TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
    GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
    CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
    GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
    AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
    ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
    GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
    ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
    TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
    GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
    ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
    GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
    GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
    GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
    GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
    ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
    GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
    GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
    CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
    GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
    CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
    CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
    GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
    TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
    CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
    CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
    TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
    GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
    CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
    GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
    CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
    CTTCGAAGTG TGTGCCACTG TTTATACCAG GTGGTTAGCC AGCGTTTCCC TCAGAACAGC
    ATCGGTGCAG TAGGAAGTGC CATGTTCCTC AGATTTATCA ATCCTGCCAT TGTCTCACCG
    TATGAAGCAG GGATTTTAGA TAAAAAGCCA CCACCTAGAA TCGAAAGGGG CTTGAAGTTA
    ATGTCAAAGA TACTTCAGAG TATTGCCAAT CATGTTCTCT TCACAAAAGA AGAACATATG
    CGGCCTTTCA ATGATTTTGT GAAAAGCAAC TTTGATGCAG CACGCAGGTT TTTCCTTGAT
    ATAGCATCTG ATTGTCCTAC AAGTGATGCA GTAAATCATA GTCTTTCCTT CATAAGTGAC
    GGCAATGTGC TTGCTTTACA TCGTCTACTC TGGAACAATC AGGAGAAAAT TGGGCAGTAT
    CTTTCCAGCA ACAGGGATCA TAAAGCTGTT GGAAGACGAC CTTTTGATAA GATGGCAACA
    CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC AAACCTGTGG CAGATACACA CTGGTCCAGC
    CTTAACCTTA CCAGTTCAAA GTTTGAGGAA TTTATGACTA GGCATCAGGT ACATGAAAAA
    GAAGAATTCA AGGCTTTGAA AACGTTAAGT ATTTTCTACC AAGCTGGGAC TTCCAAAGCT
    GGGAATCCTA TTTTTTATTA TGTTGCACGG AGGTTCAAAA CTGGTCAAAT CAATGGTGAT
    TTGCTGATAT ACCATGTCTT ACTGACTTTA AAGCCATATT ATGCAAAGCC ATATGAAATT
    GTAGTGGACC TTACCCATAC CGGGCCTAGC AATCGCTTTA AAACAGACTT TCTCTCTAAG
    TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC GACAACGTCT CCGCAGTCTA TATCTATAAC
    TGTAACTCCT GGGTCAGGGA GTACACCAAG TATCATGAGC GGCTGCTGAC TGGCCTCAAA
    GGTAGCAAAA GGCTTGTTTT CATAGACTGT CCTGGGAAAC TGGCTGAGCA CATAGAGCAT
    GAACAACAGA AACTACCTGC TGCCACCTTG GCTTTAGAAG AGGACCTGAA GGTATTCCAC
    AATGCTCTCA AGCTAGCTCA CAAAGACACC AAAGTTTCTA TTAAAGTTGG TTCTACTGCT
    GTCCAAGTAA CTTCAGCAGA GCGAACAAAA GTCCTAGGGC AATCAGTCTT TCTAAATGAC
    ATTTATTATG CTTCGGAAAT TGAAGAAATC TGCCTAGTAG ATGAGAACCA GTTCACCTTA
    ACCATTGCAA ACCAGGGCAC GCCGCTCACC TTCATGCACC AGGAGTGTGA AGCCATTGTC
    CAGTCTATCA TTCATATCCG GACCCGCTGG GAACTGTCAC AGCCCGACTC TATCCCCCAA
    CACACCAAGA TTCGGCCAAA AGATGTCCCT GGGACACTGC TCAATATCGC ATTACTTAAT
    TTAGGCAGTT CTGACCCGAG TTTACGGTCA GCTGCCTATA ATCTTCTGTG TGCCTTAACT
    TGTACCTTTA ATTTAAAAAT CGAGGGCCAG TTACTAGAGA CATCAGGTTT ATGTATCCCT
    GCCAACAACA CCCTCTTTAT TGTCTCTATT AGTAAGACAC TGGCAGCCAA TGAGCCACAC
    CTCACGTTAG AATTTTTGGA AGAGTGTATT TCTGGATTTA GCAAATCTAG TATTGAATTG
    AAACACCTTT GTTTGGAATA CATGACTCCA TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG
    CATAATGATG ATGCCAAACG ACAAAGAGTT ACTGCTATTC TTGACAAGCT GATAACAATG
    ACCATCAATG AAAAACAGAT GTACCCATCT ATTCAAGCAA AAATATGGGG AAGCCTTGGG
    CAGATTACAG ATCTGCTTGA TGTTGTACTA GACAGTTTCA TCAAAACCAG TGCAACAGGT
    GGCTTGGGAT CAATAAAAGC TGAGGTGATG GCAGATACTG CTGTAGCTTT GGCTTCTGGA
    AATGTGAAAT TGGTTTCAAG CAAGGTTATT GGAAGGATGT GCAAAATAAT TGACAAGACA
    TGCTTATCTC CAACTCCTAC TTTAGAACAA CATCTTATGT GGGATGATAT TGCTATTTTA
    GCACGCTACA TGCTGATGCT GTCCTTCAAC AATTCCCTTG ATGTGGCAGC TCATCTTCCC
    TACCTCTTCC ACGTTGTTAC TTTCTTAGTA GCCACAGGTC CGCTCTCCCT TAGAGCTTCC
    ACACATGGAC TGGTCATTAA TATCATTCAC TCTCTGTGTA CTTGTTCACA GCTTCATTTT
    AGTGAAGAGA CCAAGCAAGT TTTGAGACTC AGTCTGACAG AGTTCTCATT ACCCAAATTT
    TACTTGCTGT TTGGCATTAG CAAAGTCAAG TCAGCTGCTG TCATTGCCTT CCGTTCCAGT
    TACCGGGACA GGTCATTCTC TCCTGGCTCC TATGAGAGAG AGACTTTTGC TTTGACATCC
    TTGGAAACAG TCACAGAAGC TTTGTTGGAG ATCATGGAGG CATGCATGAG AGATATTCCA
    ACGTGCAAGT GGCTGGACCA GTGGACAGAA CTAGCTCAAA GATTTGCATT CCAATATAAT
    CCATCCCTGC AACCAAGAGC TCTTGTTGTC TTTGGGTGTA TTAGCAAACG AGTGTCTCAT
    GGGCAGATAA AGCAGATAAT CCGTATTCTT AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA
    CCTGACACTT ACAACAGTCA AGTTCTGATA GAAGCTACAG TAATAGCACT AACCAAATTA
    CAGCCACTTC TTAATAAGGA CTCGCCTCTG CACAAAGCCC TCTTTTGGGT AGCTGTGGCT
    GTGCTGCAGC TTGATGAGGT CAACTTGTAT TCAGCAGGTA CCGCACTTCT TGAACAAAAC
    CTGCATACTT TAGATAGTCT CCGTATATTC AATGACAAGA GTCCAGAGGA AGTATTTATG
    GCAATCCGGA ATCCTCTGGA GTGGCACTGC AAGCAAATGG ATCATTTTGT TGGACTCAAT
    TTCAACTCTA ACTTTAACTT TGCATTGGTT GGACACCTTT TAAAAGGGTA CAGGCATCCT
    TCACCTGCTA TTGTTGCAAG AACAGTCAGA ATTTTACATA CACTACTAAC TCTGGTTAAC
    AAACACAGAA ATTGTGACAA ATTTGAAGTG AATACACAGA GCGTGGCCTA CTTAGCAGCT
    TTACTTACAG TGTCTGAAGA AGTTCGAAGT CGCTGCAGCC TAAAACATAG AAAGTCACTT
    CTTCTTACTG ATATTTCAAT GGAAAATGTT CCTATGGATA CATATCCCAT TCATCATGGT
    GACCCTTCCT ATAGGACACT AAAGGAGACT CAGCCATGGT CCTCTCCCAA AGGTTCTGAA
    GGATACCTTG CAGCCACCTA TCCAACTGTC GGCCAGACCA GTCCCCGAGC CAGGAAATCC
    ATGAGCCTGG ACATGGGGCA ACCTTCTCAG GCCAACACTA AGAAGTTGCT TGGAACAAGG
    AAAAGTTTTG ATCACTTGAT ATCAGACACA AAGGCTCCTA AAAGGCAAGA AATGGAATCA
    GGGATCACAA CACCCCCCAA AATGAGGAGA GTAGCAGAAA CTGATTATGA AATGGAAACT
    CAGAGGATTT CCTCATCACA ACAGCACCCA CATTTACGTA AAGTTTCAGT GTCTGAATCA
    AATGTTCTCT TGGATGAAGA AGTACTTACT GATCCGAAGA TCCAGGCGCT GCTTCTTACT
    GTTCTAGCTA CACTGGTAAA ATATACCACA GATGAGTTTG ATCAACGAAT TCTTTATGAA
    TACTTAGCAG AGGCCAGTGT TGTGTTTCCC AAAGTCTTTC CTGTTGTGCA TAATTTGTTG
    GACTCTAAGA TCAACACCCT GTTATCATTG TGCCAAGATC CAAATTTGTT AAATCCAATC
    CATGGAATTG TGCAGAGTGT GGTGTACCAT GAAGAATCCC CACCACAATA CCAAACATCT
    TACCTGCAAA GTTTTGGTTT TAATGGCTTG TGGCGGTTTG CAGGACCGTT TTCAAAGCAA
    ACACAAATTC CAGACTATGC TGAGCTTATT GTTAAGTTTC TTGATGCCTT GATTGACACG
    TACCTGCCTG GAATTGATGA AGAAACCAGT GAAGAATCCC TCCTGACTCC CACATCTCCT
    TACCCTCCTG CACTGCAGAG CCAGCTTAGT ATCACTGCCA ACCTTAACCT TTCTAATTCC
    ATGACCTCAC TTGCAACTTC CCAGCATTCC CCAGCTTCTC TGCCTTGCTC TAAATCAGCA
    GTTTTCATGC AGCTGTTCCC TCATCAAGGA ATCGACAAGG AGAACGTTGA ACTCTCCCCT
    ACCACTGGCC ACTGTAACAG TGGACGAACT CGCCACGGAT CCGCAAGCCA AGTGCAGAAG
    CAAAGAAGCG CTGGCAGTTT CAAACGTAAT AGCATTAAGA AGATCGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005258041.1
    CDS334..8844
    Translation

    Target ORF information:

    RefSeq Version XM_005257984.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005257984.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    TCCGCATTGG ATTGGTGGCC TAAGATTGAT GCTGTGTATT GTCACTCGGT TGAACTTCGA
    AATATGTTTG GTGAAACACT TCATAAAGCA GTGCAAGGTT GTGGAGCACA CCCAGCAATA
    CGAATGGCAC CGAGTCTTAC ATTTAAAGAA AAAGTAACAA GCCTTAAATT TAAAGAAAAA
    CCTACAGACC TGGAGACAAG AAGCTATAAG TATCTTCTCT TGTCCATGGT GAAACTAATT
    CATGCAGATC CAAAGCTCTT GCTTTGTAAT CCAAGAAAAC AGGGGCCCGA AACCCAAGGC
    AGTACAGCAG AATTAATTAC AGGGCTCGTC CAACTGGTCC CTCAGTCACA CATGCCAGAG
    ATTGCTCAGG AAGCAATGGA GGCTCTGCTG GTTCTTCATC AGTTAGATAG CATTGATTTG
    TGGAATCCTG ATGCTCCTGT AGAAACATTT TGGGAGATTA GCTCACAAAT GCTTTTTTAC
    ATCTGCAAGA AATTAACTAG TCATCAAATG CTTAGTAGCA CAGAAATTCT CAAGTGGTTG
    CGGGAAATAT TGATCTGCAG GAATAAATTT CTTCTTAAAA ATAAGCAGGC AGATAGAAGT
    TCCTGTCACT TTCTCCTTTT TTACGGGGTA GGATGTGATA TTCCTTCTAG TGGAAATACC
    AGTCAAATGT CCATGGATCA TGAAGAATTA CTACGTACTC CTGGAGCCTC TCTCCGGAAG
    GGAAAAGGGA ACTCCTCTAT GGATAGTGCA GCAGGATGCA GCGGAACCCC CCCGATTTGC
    CGACAAGCCC AGACCAAACT AGAAGTGGCC CTGTACATGT TTCTGTGGAA CCCTGACACT
    GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC CGCCACCTCT GTGAGGAAGC AGATATCCGG
    TGTGGGGTGG ATGAAGTGTC AGTGCATAAC CTCTTGCCCA ACTATAACAC ATTCATGGAG
    TTTGCCTCTG TCAGCAATAT GATGTCAACA GGAAGAGCAG CACTTCAGAA AAGAGTGATG
    GCACTGCTGA GGCGCATTGA GCATCCCACT GCAGGAAACA CTGAGGCTTG GGAAGATACA
    CATGCAAAAT GGGAACAAGC AACAAAGCTA ATCCTTAACT ATCCAAAAGC CAAAATGGAA
    GATGGCCAGG CTGCTGAAAG CCTTCACAAG ACCATTGTTA AGAGGCGAAT GTCCCATGTG
    AGTGGAGGAG GATCCATAGA TTTGTCTGAC ACAGACTCCC TACAGGAATG GATCAACATG
    ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG
    GCAACCTATA GCCCACCCAT GGGTCCAGTC AGTGAACGTA AGGGTTCTAT GATTTCAGTG
    ATGTCTTCAG AGGGAAACGC AGATACACCT GTCAGCAAAT TTATGGATCG GCTGTTGTCC
    TTAATGGTGT GTAACCATGA GAAAGTGGGA CTTCAAATAC GGACCAATGT TAAGGATCTG
    GTGGGTCTAG AATTGAGTCC TGCTCTGTAT CCAATGCTAT TTAACAAATT GAAGAATACC
    ATCAGCAAGT TTTTTGACTC CCAAGGACAG GTTTTATTGA CTGATACCAA TACTCAATTT
    GTAGAACAAA CCATAGCTAT AATGAAGAAC TTGCTAGATA ATCATACTGA AGGCAGCTCT
    GAACATCTAG GGCAAGCTAG CATTGAAACA ATGATGTTAA ATCTGGTCAG GTATGTTCGT
    GTGCTTGGGA ATATGGTCCA TGCAATTCAA ATAAAAACGA AACTGTGTCA ATTAGTTGAA
    GTAATGATGG CAAGGAGAGA TGACCTCTCA TTTTGCCAAG AGATGAAATT TAGGAATAAG
    ATGGTAGAAT ACCTGACAGA CTGGGTTATG GGAACATCAA ACCAAGCAGC AGATGATGAT
    GTAAAATGTC TTACAAGAGA TTTGGACCAG GCAAGCATGG AAGCAGTAGT TTCACTTCTA
    GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA GATGGTGTGG AATTGATGGA AGCCAAATCA
    CAGTTATTTC TTAAATACTT CACATTATTT ATGAACCTTT TGAATGACTG CAGTGAAGTT
    GAAGATGAAA GTGCGCAAAC AGGTGGCAGG AAACGTGGCA TGTCTCGGAG GCTGGCATCA
    CTGAGGCACT GTACGGTCCT TGCAATGTCA AACTTACTCA ATGCCAACGT AGACAGTGGT
    CTCATGCACT CCATAGGCTT AGGTTACCAC AAGGATCTCC AGACAAGAGC TACATTTATG
    GAAGTTCTGA CAAAAATCCT TCAACAAGGC ACAGAATTTG ACACACTTGC AGAAACAGTA
    TTGGCTGATC GGTTTGAGAG ATTGGTGGAA CTGGTCACAA TGATGGGTGA TCAAGGAGAA
    CTCCCTATAG CGATGGCTCT GGCCAATGTG GTTCCTTGTT CTCAGTGGGA TGAACTAGCT
    CGAGTTCTGG TTACTCTGTT TGATTCTCGG CATTTACTCT ACCAACTGCT CTGGAACATG
    TTTTCTAAAG AAGTAGAATT GGCAGACTCC ATGCAGACTC TCTTCCGAGG CAACAGCTTG
    GCCAGTAAAA TAATGACATT CTGTTTCAAG GTATATGGTG CTACCTATCT ACAAAAACTC
    CTGGATCCTT TATTACGAAT TGTGATCACA TCCTCTGATT GGCAACATGT TAGCTTTGAA
    GTGGATCCTA CCAGGTTAGA ACCATCAGAG AGCCTTGAGG AAAACCAGCG GAACCTCCTT
    CAGATGACTG AAAAGTTCTT CCATGCCATC ATCAGTTCCT CCTCAGAATT CCCCCCTCAA
    CTTCGAAGTG TGTGCCACTG TTTATACCAG GTGGTTAGCC AGCGTTTCCC TCAGAACAGC
    ATCGGTGCAG TAGGAAGTGC CATGTTCCTC AGATTTATCA ATCCTGCCAT TGTCTCACCG
    TATGAAGCAG GGATTTTAGA TAAAAAGCCA CCACCTAGAA TCGAAAGGGG CTTGAAGTTA
    ATGTCAAAGA TACTTCAGAG TATTGCCAAT CATGTTCTCT TCACAAAAGA AGAACATATG
    CGGCCTTTCA ATGATTTTGT GAAAAGCAAC TTTGATGCAG CACGCAGGTT TTTCCTTGAT
    ATAGCATCTG ATTGTCCTAC AAGTGATGCA GTAAATCATA GTCTTTCCTT CATAAGTGAC
    GGCAATGTGC TTGCTTTACA TCGTCTACTC TGGAACAATC AGGAGAAAAT TGGGCAGTAT
    CTTTCCAGCA ACAGGGATCA TAAAGCTGTT GGAAGACGAC CTTTTGATAA GATGGCAACA
    CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC AAACCTGTGG CAGATACACA CTGGTCCAGC
    CTTAACCTTA CCAGTTCAAA GTTTGAGGAA TTTATGACTA GGCATCAGGT ACATGAAAAA
    GAAGAATTCA AGGCTTTGAA AACGTTAAGT ATTTTCTACC AAGCTGGGAC TTCCAAAGCT
    GGGAATCCTA TTTTTTATTA TGTTGCACGG AGGTTCAAAA CTGGTCAAAT CAATGGTGAT
    TTGCTGATAT ACCATGTCTT ACTGACTTTA AAGCCATATT ATGCAAAGCC ATATGAAATT
    GTAGTGGACC TTACCCATAC CGGGCCTAGC AATCGCTTTA AAACAGACTT TCTCTCTAAG
    TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC GACAACGTCT CCGCAGTCTA TATCTATAAC
    TGTAACTCCT GGGTCAGGGA GTACACCAAG TATCATGAGC GGCTGCTGAC TGGCCTCAAA
    GGTAGCAAAA GGCTTGTTTT CATAGACTGT CCTGGGAAAC TGGCTGAGCA CATAGAGCAT
    GAACAACAGA AACTACCTGC TGCCACCTTG GCTTTAGAAG AGGACCTGAA GGTATTCCAC
    AATGCTCTCA AGCTAGCTCA CAAAGACACC AAAGTTTCTA TTAAAGTTGG TTCTACTGCT
    GTCCAAGTAA CTTCAGCAGA GCGAACAAAA GTCCTAGGGC AATCAGTCTT TCTAAATGAC
    ATTTATTATG CTTCGGAAAT TGAAGAAATC TGCCTAGTAG ATGAGAACCA GTTCACCTTA
    ACCATTGCAA ACCAGGGCAC GCCGCTCACC TTCATGCACC AGGAGTGTGA AGCCATTGTC
    CAGTCTATCA TTCATATCCG GACCCGCTGG GAACTGTCAC AGCCCGACTC TATCCCCCAA
    CACACCAAGA TTCGGCCAAA AGATGTCCCT GGGACACTGC TCAATATCGC ATTACTTAAT
    TTAGGCAGTT CTGACCCGAG TTTACGGTCA GCTGCCTATA ATCTTCTGTG TGCCTTAACT
    TGTACCTTTA ATTTAAAAAT CGAGGGCCAG TTACTAGAGA CATCAGGTTT ATGTATCCCT
    GCCAACAACA CCCTCTTTAT TGTCTCTATT AGTAAGACAC TGGCAGCCAA TGAGCCACAC
    CTCACGTTAG AATTTTTGGA AGAGTGTATT TCTGGATTTA GCAAATCTAG TATTGAATTG
    AAACACCTTT GTTTGGAATA CATGACTCCA TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG
    CATAATGATG ATGCCAAACG ACAAAGAGTT ACTGCTATTC TTGACAAGCT GATAACAATG
    ACCATCAATG AAAAACAGAT GTACCCATCT ATTCAAGCAA AAATATGGGG AAGCCTTGGG
    CAGATTACAG ATCTGCTTGA TGTTGTACTA GACAGTTTCA TCAAAACCAG TGCAACAGGT
    GGCTTGGGAT CAATAAAAGC TGAGGTGATG GCAGATACTG CTGTAGCTTT GGCTTCTGGA
    AATGTGAAAT TGGTTTCAAG CAAGGTTATT GGAAGGATGT GCAAAATAAT TGACAAGACA
    TGCTTATCTC CAACTCCTAC TTTAGAACAA CATCTTATGT GGGATGATAT TGCTATTTTA
    GCACGCTACA TGCTGATGCT GTCCTTCAAC AATTCCCTTG ATGTGGCAGC TCATCTTCCC
    TACCTCTTCC ACGTTGTTAC TTTCTTAGTA GCCACAGGTC CGCTCTCCCT TAGAGCTTCC
    ACACATGGAC TGGTCATTAA TATCATTCAC TCTCTGTGTA CTTGTTCACA GCTTCATTTT
    AGTGAAGAGA CCAAGCAAGT TTTGAGACTC AGTCTGACAG AGTTCTCATT ACCCAAATTT
    TACTTGCTGT TTGGCATTAG CAAAGTCAAG TCAGCTGCTG TCATTGCCTT CCGTTCCAGT
    TACCGGGACA GGTCATTCTC TCCTGGCTCC TATGAGAGAG AGACTTTTGC TTTGACATCC
    TTGGAAACAG TCACAGAAGC TTTGTTGGAG ATCATGGAGG CATGCATGAG AGATATTCCA
    ACGTGCAAGT GGCTGGACCA GTGGACAGAA CTAGCTCAAA GATTTGCATT CCAATATAAT
    CCATCCCTGC AACCAAGAGC TCTTGTTGTC TTTGGGTGTA TTAGCAAACG AGTGTCTCAT
    GGGCAGATAA AGCAGATAAT CCGTATTCTT AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA
    CCTGACACTT ACAACAGTCA AGTTCTGATA GAAGCTACAG TAATAGCACT AACCAAATTA
    CAGCCACTTC TTAATAAGGA CTCGCCTCTG CACAAAGCCC TCTTTTGGGT AGCTGTGGCT
    GTGCTGCAGC TTGATGAGGT CAACTTGTAT TCAGCAGGTA CCGCACTTCT TGAACAAAAC
    CTGCATACTT TAGATAGTCT CCGTATATTC AATGACAAGA GTCCAGAGGA AGTATTTATG
    GCAATCCGGA ATCCTCTGGA GTGGCACTGC AAGCAAATGG ATCATTTTGT TGGACTCAAT
    TTCAACTCTA ACTTTAACTT TGCATTGGTT GGACACCTTT TAAAAGGGTA CAGGCATCCT
    TCACCTGCTA TTGTTGCAAG AACAGTCAGA ATTTTACATA CACTACTAAC TCTGGTTAAC
    AAACACAGAA ATTGTGACAA ATTTGAAGTG AATACACAGA GCGTGGCCTA CTTAGCAGCT
    TTACTTACAG TGTCTGAAGA AGTTCGAAGT CGCTGCAGCC TAAAACATAG AAAGTCACTT
    CTTCTTACTG ATATTTCAAT GGAAAATGTT CCTATGGATA CATATCCCAT TCATCATGGT
    GACCCTTCCT ATAGGACACT AAAGGAGACT CAGCCATGGT CCTCTCCCAA AGGTTCTGAA
    GGATACCTTG CAGCCACCTA TCCAACTGTC GGCCAGACCA GTCCCCGAGC CAGGAAATCC
    ATGAGCCTGG ACATGGGGCA ACCTTCTCAG GCCAACACTA AGAAGTTGCT TGGAACAAGG
    AAAAGTTTTG ATCACTTGAT ATCAGACACA AAGGCTCCTA AAAGGCAAGA AATGGAATCA
    GGGATCACAA CACCCCCCAA AATGAGGAGA GTAGCAGAAA CTGATTATGA AATGGAAACT
    CAGAGGATTT CCTCATCACA ACAGCACCCA CATTTACGTA AAGTTTCAGT GTCTGAATCA
    AATGTTCTCT TGGATGAAGA AGTACTTACT GATCCGAAGA TCCAGGCGCT GCTTCTTACT
    GTTCTAGCTA CACTGGTAAA ATATACCACA GATGAGTTTG ATCAACGAAT TCTTTATGAA
    TACTTAGCAG AGGCCAGTGT TGTGTTTCCC AAAGTCTTTC CTGTTGTGCA TAATTTGTTG
    GACTCTAAGA TCAACACCCT GTTATCATTG TGCCAAGATC CAAATTTGTT AAATCCAATC
    CATGGAATTG TGCAGAGTGT GGTGTACCAT GAAGAATCCC CACCACAATA CCAAACATCT
    TACCTGCAAA GTTTTGGTTT TAATGGCTTG TGGCGGTTTG CAGGACCGTT TTCAAAGCAA
    ACACAAATTC CAGACTATGC TGAGCTTATT GTTAAGTTTC TTGATGCCTT GATTGACACG
    TACCTGCCTG GAATTGATGA AGAAACCAGT GAAGAATCCC TCCTGACTCC CACATCTCCT
    TACCCTCCTG CACTGCAGAG CCAGCTTAGT ATCACTGCCA ACCTTAACCT TTCTAATTCC
    ATGACCTCAC TTGCAACTTC CCAGCATTCC CCAGCTTCTC TGCCTTGCTC TAAATCAGCA
    GTTTTCATGC AGCTGTTCCC TCATCAAGGA ATCGACAAGG AGAACGTTGA ACTCTCCCCT
    ACCACTGGCC ACTGTAACAG TGGACGAACT CGCCACGGAT CCGCAAGCCA AGTGCAGAAG
    CAAAGAAGCG CTGGCAGTTT CAAACGTAAT AGCATTAAGA AGATCGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu50433
    Clone ID Related Accession (Same CDS sequence) XM_006721926.3
    Accession Version XM_006721926.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurofibromin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721926.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    481
    541
    601
    661
    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
    CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
    CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
    AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
    AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
    GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
    CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
    GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
    CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
    GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
    AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
    CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
    TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
    AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
    TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
    AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
    TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
    TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
    ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
    AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
    ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
    ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
    TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
    CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
    TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
    CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
    ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
    ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
    CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
    GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
    TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
    CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
    ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
    CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
    AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
    GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
    CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
    CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
    AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
    TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
    GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
    GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
    GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
    CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
    ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
    CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
    TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
    TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
    TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
    GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
    AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
    GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
    ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
    GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
    CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
    ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
    AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
    CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
    ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
    ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
    CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
    TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
    CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
    TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
    ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
    AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
    AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
    TCCCCAGATA CTGCAGCCAC TGAATTCCTT CCTGCTTCCC CGCTGATAAA CCTTTTAGCA
    TTTTGGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006721989.1
    CDS334..8805
    Translation

    Target ORF information:

    RefSeq Version XM_006721926.3
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721926.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
    CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
    CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
    AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
    AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
    GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
    CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
    GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
    CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
    GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
    AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
    CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
    TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
    AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
    TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
    AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
    TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
    TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
    ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
    AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
    ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
    ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
    TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
    CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
    TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
    CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
    ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
    ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
    CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
    GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
    TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
    CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
    ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
    CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
    AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
    GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
    CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
    CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
    AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
    TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
    GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
    GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
    GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
    CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
    ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
    CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
    TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
    TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
    TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
    GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
    AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
    GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
    ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
    GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
    CAGGCCAACA CTAAGAAGTT GCTTGGAACA AGGAAAAGTT TTGATCACTT GATATCAGAC
    ACAAAGGCTC CTAAAAGGCA AGAAATGGAA TCAGGGATCA CAACACCCCC CAAAATGAGG
    AGAGTAGCAG AAACTGATTA TGAAATGGAA ACTCAGAGGA TTTCCTCATC ACAACAGCAC
    CCACATTTAC GTAAAGTTTC AGTGTCTGAA TCAAATGTTC TCTTGGATGA AGAAGTACTT
    ACTGATCCGA AGATCCAGGC GCTGCTTCTT ACTGTTCTAG CTACACTGGT AAAATATACC
    ACAGATGAGT TTGATCAACG AATTCTTTAT GAATACTTAG CAGAGGCCAG TGTTGTGTTT
    CCCAAAGTCT TTCCTGTTGT GCATAATTTG TTGGACTCTA AGATCAACAC CCTGTTATCA
    TTGTGCCAAG ATCCAAATTT GTTAAATCCA ATCCATGGAA TTGTGCAGAG TGTGGTGTAC
    CATGAAGAAT CCCCACCACA ATACCAAACA TCTTACCTGC AAAGTTTTGG TTTTAATGGC
    TTGTGGCGGT TTGCAGGACC GTTTTCAAAG CAAACACAAA TTCCAGACTA TGCTGAGCTT
    ATTGTTAAGT TTCTTGATGC CTTGATTGAC ACGTACCTGC CTGGAATTGA TGAAGAAACC
    AGTGAAGAAT CCCTCCTGAC TCCCACATCT CCTTACCCTC CTGCACTGCA GAGCCAGCTT
    AGTATCACTG CCAACCTTAA CCTTTCTAAT TCCATGACCT CACTTGCAAC TTCCCAGCAT
    TCCCCAGATA CTGCAGCCAC TGAATTCCTT CCTGCTTCCC CGCTGATAAA CCTTTTAGCA
    TTTTGGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59245
    Clone ID Related Accession (Same CDS sequence) XM_011524852.2
    Accession Version XM_011524852.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8601bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurofibromin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGGCAGA TAGAAGTTCC TGTCACTTTC TCCTTTTTTA CGGGGTAGGA
    TGTGATATTC CTTCTAGTGG AAATACCAGT CAAATGTCCA TGGATCATGA AGAATTACTA
    CGTACTCCTG GAGCCTCTCT CCGGAAGGGA AAAGGGAACT CCTCTATGGA TAGTGCAGCA
    GGATGCAGCG GAACCCCCCC GATTTGCCGA CAAGCCCAGA CCAAACTAGA AGTGGCCCTG
    TACATGTTTC TGTGGAACCC TGACACTGAA GCTGTTCTGG TTGCCATGTC CTGTTTCCGC
    CACCTCTGTG AGGAAGCAGA TATCCGGTGT GGGGTGGATG AAGTGTCAGT GCATAACCTC
    TTGCCCAACT ATAACACATT CATGGAGTTT GCCTCTGTCA GCAATATGAT GTCAACAGGA
    AGAGCAGCAC TTCAGAAAAG AGTGATGGCA CTGCTGAGGC GCATTGAGCA TCCCACTGCA
    GGAAACACTG AGGCTTGGGA AGATACACAT GCAAAATGGG AACAAGCAAC AAAGCTAATC
    CTTAACTATC CAAAAGCCAA AATGGAAGAT GGCCAGGCTG CTGAAAGCCT TCACAAGACC
    ATTGTTAAGA GGCGAATGTC CCATGTGAGT GGAGGAGGAT CCATAGATTT GTCTGACACA
    GACTCCCTAC AGGAATGGAT CAACATGACT GGCTTCCTTT GTGCCCTTGG GGGAGTGTGC
    CTCCAGCAGA GAAGCAATTC TGGCCTGGCA ACCTATAGCC CACCCATGGG TCCAGTCAGT
    GAACGTAAGG GTTCTATGAT TTCAGTGATG TCTTCAGAGG GAAACGCAGA TACACCTGTC
    AGCAAATTTA TGGATCGGCT GTTGTCCTTA ATGGTGTGTA ACCATGAGAA AGTGGGACTT
    CAAATACGGA CCAATGTTAA GGATCTGGTG GGTCTAGAAT TGAGTCCTGC TCTGTATCCA
    ATGCTATTTA ACAAATTGAA GAATACCATC AGCAAGTTTT TTGACTCCCA AGGACAGGTT
    TTATTGACTG ATACCAATAC TCAATTTGTA GAACAAACCA TAGCTATAAT GAAGAACTTG
    CTAGATAATC ATACTGAAGG CAGCTCTGAA CATCTAGGGC AAGCTAGCAT TGAAACAATG
    ATGTTAAATC TGGTCAGGTA TGTTCGTGTG CTTGGGAATA TGGTCCATGC AATTCAAATA
    AAAACGAAAC TGTGTCAATT AGTTGAAGTA ATGATGGCAA GGAGAGATGA CCTCTCATTT
    TGCCAAGAGA TGAAATTTAG GAATAAGATG GTAGAATACC TGACAGACTG GGTTATGGGA
    ACATCAAACC AAGCAGCAGA TGATGATGTA AAATGTCTTA CAAGAGATTT GGACCAGGCA
    AGCATGGAAG CAGTAGTTTC ACTTCTAGCT GGTCTCCCTC TGCAGCCTGA AGAAGGAGAT
    GGTGTGGAAT TGATGGAAGC CAAATCACAG TTATTTCTTA AATACTTCAC ATTATTTATG
    AACCTTTTGA ATGACTGCAG TGAAGTTGAA GATGAAAGTG CGCAAACAGG TGGCAGGAAA
    CGTGGCATGT CTCGGAGGCT GGCATCACTG AGGCACTGTA CGGTCCTTGC AATGTCAAAC
    TTACTCAATG CCAACGTAGA CAGTGGTCTC ATGCACTCCA TAGGCTTAGG TTACCACAAG
    GATCTCCAGA CAAGAGCTAC ATTTATGGAA GTTCTGACAA AAATCCTTCA ACAAGGCACA
    GAATTTGACA CACTTGCAGA AACAGTATTG GCTGATCGGT TTGAGAGATT GGTGGAACTG
    GTCACAATGA TGGGTGATCA AGGAGAACTC CCTATAGCGA TGGCTCTGGC CAATGTGGTT
    CCTTGTTCTC AGTGGGATGA ACTAGCTCGA GTTCTGGTTA CTCTGTTTGA TTCTCGGCAT
    TTACTCTACC AACTGCTCTG GAACATGTTT TCTAAAGAAG TAGAATTGGC AGACTCCATG
    CAGACTCTCT TCCGAGGCAA CAGCTTGGCC AGTAAAATAA TGACATTCTG TTTCAAGGTA
    TATGGTGCTA CCTATCTACA AAAACTCCTG GATCCTTTAT TACGAATTGT GATCACATCC
    TCTGATTGGC AACATGTTAG CTTTGAAGTG GATCCTACCA GGTTAGAACC ATCAGAGAGC
    CTTGAGGAAA ACCAGCGGAA CCTCCTTCAG ATGACTGAAA AGTTCTTCCA TGCCATCATC
    AGTTCCTCCT CAGAATTCCC CCCTCAACTT CGAAGTGTGT GCCACTGTTT ATACCAGGCA
    ACTTGCCACT CCCTACTGAA TAAAGCTACA GTAAAAGAAA AAAAGGAAAA CAAAAAATCA
    GTGGTTAGCC AGCGTTTCCC TCAGAACAGC ATCGGTGCAG TAGGAAGTGC CATGTTCCTC
    AGATTTATCA ATCCTGCCAT TGTCTCACCG TATGAAGCAG GGATTTTAGA TAAAAAGCCA
    CCACCTAGAA TCGAAAGGGG CTTGAAGTTA ATGTCAAAGA TACTTCAGAG TATTGCCAAT
    CATGTTCTCT TCACAAAAGA AGAACATATG CGGCCTTTCA ATGATTTTGT GAAAAGCAAC
    TTTGATGCAG CACGCAGGTT TTTCCTTGAT ATAGCATCTG ATTGTCCTAC AAGTGATGCA
    GTAAATCATA GTCTTTCCTT CATAAGTGAC GGCAATGTGC TTGCTTTACA TCGTCTACTC
    TGGAACAATC AGGAGAAAAT TGGGCAGTAT CTTTCCAGCA ACAGGGATCA TAAAGCTGTT
    GGAAGACGAC CTTTTGATAA GATGGCAACA CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC
    AAACCTGTGG CAGATACACA CTGGTCCAGC CTTAACCTTA CCAGTTCAAA GTTTGAGGAA
    TTTATGACTA GGCATCAGGT ACATGAAAAA GAAGAATTCA AGGCTTTGAA AACGTTAAGT
    ATTTTCTACC AAGCTGGGAC TTCCAAAGCT GGGAATCCTA TTTTTTATTA TGTTGCACGG
    AGGTTCAAAA CTGGTCAAAT CAATGGTGAT TTGCTGATAT ACCATGTCTT ACTGACTTTA
    AAGCCATATT ATGCAAAGCC ATATGAAATT GTAGTGGACC TTACCCATAC CGGGCCTAGC
    AATCGCTTTA AAACAGACTT TCTCTCTAAG TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC
    GACAACGTCT CCGCAGTCTA TATCTATAAC TGTAACTCCT GGGTCAGGGA GTACACCAAG
    TATCATGAGC GGCTGCTGAC TGGCCTCAAA GGTAGCAAAA GGCTTGTTTT CATAGACTGT
    CCTGGGAAAC TGGCTGAGCA CATAGAGCAT GAACAACAGA AACTACCTGC TGCCACCTTG
    GCTTTAGAAG AGGACCTGAA GGTATTCCAC AATGCTCTCA AGCTAGCTCA CAAAGACACC
    AAAGTTTCTA TTAAAGTTGG TTCTACTGCT GTCCAAGTAA CTTCAGCAGA GCGAACAAAA
    GTCCTAGGGC AATCAGTCTT TCTAAATGAC ATTTATTATG CTTCGGAAAT TGAAGAAATC
    TGCCTAGTAG ATGAGAACCA GTTCACCTTA ACCATTGCAA ACCAGGGCAC GCCGCTCACC
    TTCATGCACC AGGAGTGTGA AGCCATTGTC CAGTCTATCA TTCATATCCG GACCCGCTGG
    GAACTGTCAC AGCCCGACTC TATCCCCCAA CACACCAAGA TTCGGCCAAA AGATGTCCCT
    GGGACACTGC TCAATATCGC ATTACTTAAT TTAGGCAGTT CTGACCCGAG TTTACGGTCA
    GCTGCCTATA ATCTTCTGTG TGCCTTAACT TGTACCTTTA ATTTAAAAAT CGAGGGCCAG
    TTACTAGAGA CATCAGGTTT ATGTATCCCT GCCAACAACA CCCTCTTTAT TGTCTCTATT
    AGTAAGACAC TGGCAGCCAA TGAGCCACAC CTCACGTTAG AATTTTTGGA AGAGTGTATT
    TCTGGATTTA GCAAATCTAG TATTGAATTG AAACACCTTT GTTTGGAATA CATGACTCCA
    TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG CATAATGATG ATGCCAAACG ACAAAGAGTT
    ACTGCTATTC TTGACAAGCT GATAACAATG ACCATCAATG AAAAACAGAT GTACCCATCT
    ATTCAAGCAA AAATATGGGG AAGCCTTGGG CAGATTACAG ATCTGCTTGA TGTTGTACTA
    GACAGTTTCA TCAAAACCAG TGCAACAGGT GGCTTGGGAT CAATAAAAGC TGAGGTGATG
    GCAGATACTG CTGTAGCTTT GGCTTCTGGA AATGTGAAAT TGGTTTCAAG CAAGGTTATT
    GGAAGGATGT GCAAAATAAT TGACAAGACA TGCTTATCTC CAACTCCTAC TTTAGAACAA
    CATCTTATGT GGGATGATAT TGCTATTTTA GCACGCTACA TGCTGATGCT GTCCTTCAAC
    AATTCCCTTG ATGTGGCAGC TCATCTTCCC TACCTCTTCC ACGTTGTTAC TTTCTTAGTA
    GCCACAGGTC CGCTCTCCCT TAGAGCTTCC ACACATGGAC TGGTCATTAA TATCATTCAC
    TCTCTGTGTA CTTGTTCACA GCTTCATTTT AGTGAAGAGA CCAAGCAAGT TTTGAGACTC
    AGTCTGACAG AGTTCTCATT ACCCAAATTT TACTTGCTGT TTGGCATTAG CAAAGTCAAG
    TCAGCTGCTG TCATTGCCTT CCGTTCCAGT TACCGGGACA GGTCATTCTC TCCTGGCTCC
    TATGAGAGAG AGACTTTTGC TTTGACATCC TTGGAAACAG TCACAGAAGC TTTGTTGGAG
    ATCATGGAGG CATGCATGAG AGATATTCCA ACGTGCAAGT GGCTGGACCA GTGGACAGAA
    CTAGCTCAAA GATTTGCATT CCAATATAAT CCATCCCTGC AACCAAGAGC TCTTGTTGTC
    TTTGGGTGTA TTAGCAAACG AGTGTCTCAT GGGCAGATAA AGCAGATAAT CCGTATTCTT
    AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA CCTGACACTT ACAACAGTCA AGTTCTGATA
    GAAGCTACAG TAATAGCACT AACCAAATTA CAGCCACTTC TTAATAAGGA CTCGCCTCTG
    CACAAAGCCC TCTTTTGGGT AGCTGTGGCT GTGCTGCAGC TTGATGAGGT CAACTTGTAT
    TCAGCAGGTA CCGCACTTCT TGAACAAAAC CTGCATACTT TAGATAGTCT CCGTATATTC
    AATGACAAGA GTCCAGAGGA AGTATTTATG GCAATCCGGA ATCCTCTGGA GTGGCACTGC
    AAGCAAATGG ATCATTTTGT TGGACTCAAT TTCAACTCTA ACTTTAACTT TGCATTGGTT
    GGACACCTTT TAAAAGGGTA CAGGCATCCT TCACCTGCTA TTGTTGCAAG AACAGTCAGA
    ATTTTACATA CACTACTAAC TCTGGTTAAC AAACACAGAA ATTGTGACAA ATTTGAAGTG
    AATACACAGA GCGTGGCCTA CTTAGCAGCT TTACTTACAG TGTCTGAAGA AGTTCGAAGT
    CGCTGCAGCC TAAAACATAG AAAGTCACTT CTTCTTACTG ATATTTCAAT GGAAAATGTT
    CCTATGGATA CATATCCCAT TCATCATGGT GACCCTTCCT ATAGGACACT AAAGGAGACT
    CAGCCATGGT CCTCTCCCAA AGGTTCTGAA GGATACCTTG CAGCCACCTA TCCAACTGTC
    GGCCAGACCA GTCCCCGAGC CAGGAAATCC ATGAGCCTGG ACATGGGGCA ACCTTCTCAG
    GCCAACACTA AGAAGTTGCT TGGAACAAGG AAAAGTTTTG ATCACTTGAT ATCAGACACA
    AAGGCTCCTA AAAGGCAAGA AATGGAATCA GGGATCACAA CACCCCCCAA AATGAGGAGA
    GTAGCAGAAA CTGATTATGA AATGGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
    CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
    GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
    GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
    AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
    TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
    GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
    TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
    GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
    GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
    ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
    CCAGCTTCTC TGCCTTGCTC TAAATCAGCA GTTTTCATGC AGCTGTTCCC TCATCAAGGA
    ATCGACAAGG AGAACGTTGA ACTCTCCCCT ACCACTGGCC ACTGTAACAG TGGACGAACT
    CGCCACGGAT CCGCAAGCCA AGTGCAGAAG CAAAGAAGCG CTGGCAGTTT CAAACGTAAT
    AGCATTAAGA AGATCGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011523154.1
    CDS334..8934
    Translation

    Target ORF information:

    RefSeq Version XM_011524852.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011524852.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGGCAGA TAGAAGTTCC TGTCACTTTC TCCTTTTTTA CGGGGTAGGA
    TGTGATATTC CTTCTAGTGG AAATACCAGT CAAATGTCCA TGGATCATGA AGAATTACTA
    CGTACTCCTG GAGCCTCTCT CCGGAAGGGA AAAGGGAACT CCTCTATGGA TAGTGCAGCA
    GGATGCAGCG GAACCCCCCC GATTTGCCGA CAAGCCCAGA CCAAACTAGA AGTGGCCCTG
    TACATGTTTC TGTGGAACCC TGACACTGAA GCTGTTCTGG TTGCCATGTC CTGTTTCCGC
    CACCTCTGTG AGGAAGCAGA TATCCGGTGT GGGGTGGATG AAGTGTCAGT GCATAACCTC
    TTGCCCAACT ATAACACATT CATGGAGTTT GCCTCTGTCA GCAATATGAT GTCAACAGGA
    AGAGCAGCAC TTCAGAAAAG AGTGATGGCA CTGCTGAGGC GCATTGAGCA TCCCACTGCA
    GGAAACACTG AGGCTTGGGA AGATACACAT GCAAAATGGG AACAAGCAAC AAAGCTAATC
    CTTAACTATC CAAAAGCCAA AATGGAAGAT GGCCAGGCTG CTGAAAGCCT TCACAAGACC
    ATTGTTAAGA GGCGAATGTC CCATGTGAGT GGAGGAGGAT CCATAGATTT GTCTGACACA
    GACTCCCTAC AGGAATGGAT CAACATGACT GGCTTCCTTT GTGCCCTTGG GGGAGTGTGC
    CTCCAGCAGA GAAGCAATTC TGGCCTGGCA ACCTATAGCC CACCCATGGG TCCAGTCAGT
    GAACGTAAGG GTTCTATGAT TTCAGTGATG TCTTCAGAGG GAAACGCAGA TACACCTGTC
    AGCAAATTTA TGGATCGGCT GTTGTCCTTA ATGGTGTGTA ACCATGAGAA AGTGGGACTT
    CAAATACGGA CCAATGTTAA GGATCTGGTG GGTCTAGAAT TGAGTCCTGC TCTGTATCCA
    ATGCTATTTA ACAAATTGAA GAATACCATC AGCAAGTTTT TTGACTCCCA AGGACAGGTT
    TTATTGACTG ATACCAATAC TCAATTTGTA GAACAAACCA TAGCTATAAT GAAGAACTTG
    CTAGATAATC ATACTGAAGG CAGCTCTGAA CATCTAGGGC AAGCTAGCAT TGAAACAATG
    ATGTTAAATC TGGTCAGGTA TGTTCGTGTG CTTGGGAATA TGGTCCATGC AATTCAAATA
    AAAACGAAAC TGTGTCAATT AGTTGAAGTA ATGATGGCAA GGAGAGATGA CCTCTCATTT
    TGCCAAGAGA TGAAATTTAG GAATAAGATG GTAGAATACC TGACAGACTG GGTTATGGGA
    ACATCAAACC AAGCAGCAGA TGATGATGTA AAATGTCTTA CAAGAGATTT GGACCAGGCA
    AGCATGGAAG CAGTAGTTTC ACTTCTAGCT GGTCTCCCTC TGCAGCCTGA AGAAGGAGAT
    GGTGTGGAAT TGATGGAAGC CAAATCACAG TTATTTCTTA AATACTTCAC ATTATTTATG
    AACCTTTTGA ATGACTGCAG TGAAGTTGAA GATGAAAGTG CGCAAACAGG TGGCAGGAAA
    CGTGGCATGT CTCGGAGGCT GGCATCACTG AGGCACTGTA CGGTCCTTGC AATGTCAAAC
    TTACTCAATG CCAACGTAGA CAGTGGTCTC ATGCACTCCA TAGGCTTAGG TTACCACAAG
    GATCTCCAGA CAAGAGCTAC ATTTATGGAA GTTCTGACAA AAATCCTTCA ACAAGGCACA
    GAATTTGACA CACTTGCAGA AACAGTATTG GCTGATCGGT TTGAGAGATT GGTGGAACTG
    GTCACAATGA TGGGTGATCA AGGAGAACTC CCTATAGCGA TGGCTCTGGC CAATGTGGTT
    CCTTGTTCTC AGTGGGATGA ACTAGCTCGA GTTCTGGTTA CTCTGTTTGA TTCTCGGCAT
    TTACTCTACC AACTGCTCTG GAACATGTTT TCTAAAGAAG TAGAATTGGC AGACTCCATG
    CAGACTCTCT TCCGAGGCAA CAGCTTGGCC AGTAAAATAA TGACATTCTG TTTCAAGGTA
    TATGGTGCTA CCTATCTACA AAAACTCCTG GATCCTTTAT TACGAATTGT GATCACATCC
    TCTGATTGGC AACATGTTAG CTTTGAAGTG GATCCTACCA GGTTAGAACC ATCAGAGAGC
    CTTGAGGAAA ACCAGCGGAA CCTCCTTCAG ATGACTGAAA AGTTCTTCCA TGCCATCATC
    AGTTCCTCCT CAGAATTCCC CCCTCAACTT CGAAGTGTGT GCCACTGTTT ATACCAGGCA
    ACTTGCCACT CCCTACTGAA TAAAGCTACA GTAAAAGAAA AAAAGGAAAA CAAAAAATCA
    GTGGTTAGCC AGCGTTTCCC TCAGAACAGC ATCGGTGCAG TAGGAAGTGC CATGTTCCTC
    AGATTTATCA ATCCTGCCAT TGTCTCACCG TATGAAGCAG GGATTTTAGA TAAAAAGCCA
    CCACCTAGAA TCGAAAGGGG CTTGAAGTTA ATGTCAAAGA TACTTCAGAG TATTGCCAAT
    CATGTTCTCT TCACAAAAGA AGAACATATG CGGCCTTTCA ATGATTTTGT GAAAAGCAAC
    TTTGATGCAG CACGCAGGTT TTTCCTTGAT ATAGCATCTG ATTGTCCTAC AAGTGATGCA
    GTAAATCATA GTCTTTCCTT CATAAGTGAC GGCAATGTGC TTGCTTTACA TCGTCTACTC
    TGGAACAATC AGGAGAAAAT TGGGCAGTAT CTTTCCAGCA ACAGGGATCA TAAAGCTGTT
    GGAAGACGAC CTTTTGATAA GATGGCAACA CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC
    AAACCTGTGG CAGATACACA CTGGTCCAGC CTTAACCTTA CCAGTTCAAA GTTTGAGGAA
    TTTATGACTA GGCATCAGGT ACATGAAAAA GAAGAATTCA AGGCTTTGAA AACGTTAAGT
    ATTTTCTACC AAGCTGGGAC TTCCAAAGCT GGGAATCCTA TTTTTTATTA TGTTGCACGG
    AGGTTCAAAA CTGGTCAAAT CAATGGTGAT TTGCTGATAT ACCATGTCTT ACTGACTTTA
    AAGCCATATT ATGCAAAGCC ATATGAAATT GTAGTGGACC TTACCCATAC CGGGCCTAGC
    AATCGCTTTA AAACAGACTT TCTCTCTAAG TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC
    GACAACGTCT CCGCAGTCTA TATCTATAAC TGTAACTCCT GGGTCAGGGA GTACACCAAG
    TATCATGAGC GGCTGCTGAC TGGCCTCAAA GGTAGCAAAA GGCTTGTTTT CATAGACTGT
    CCTGGGAAAC TGGCTGAGCA CATAGAGCAT GAACAACAGA AACTACCTGC TGCCACCTTG
    GCTTTAGAAG AGGACCTGAA GGTATTCCAC AATGCTCTCA AGCTAGCTCA CAAAGACACC
    AAAGTTTCTA TTAAAGTTGG TTCTACTGCT GTCCAAGTAA CTTCAGCAGA GCGAACAAAA
    GTCCTAGGGC AATCAGTCTT TCTAAATGAC ATTTATTATG CTTCGGAAAT TGAAGAAATC
    TGCCTAGTAG ATGAGAACCA GTTCACCTTA ACCATTGCAA ACCAGGGCAC GCCGCTCACC
    TTCATGCACC AGGAGTGTGA AGCCATTGTC CAGTCTATCA TTCATATCCG GACCCGCTGG
    GAACTGTCAC AGCCCGACTC TATCCCCCAA CACACCAAGA TTCGGCCAAA AGATGTCCCT
    GGGACACTGC TCAATATCGC ATTACTTAAT TTAGGCAGTT CTGACCCGAG TTTACGGTCA
    GCTGCCTATA ATCTTCTGTG TGCCTTAACT TGTACCTTTA ATTTAAAAAT CGAGGGCCAG
    TTACTAGAGA CATCAGGTTT ATGTATCCCT GCCAACAACA CCCTCTTTAT TGTCTCTATT
    AGTAAGACAC TGGCAGCCAA TGAGCCACAC CTCACGTTAG AATTTTTGGA AGAGTGTATT
    TCTGGATTTA GCAAATCTAG TATTGAATTG AAACACCTTT GTTTGGAATA CATGACTCCA
    TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG CATAATGATG ATGCCAAACG ACAAAGAGTT
    ACTGCTATTC TTGACAAGCT GATAACAATG ACCATCAATG AAAAACAGAT GTACCCATCT
    ATTCAAGCAA AAATATGGGG AAGCCTTGGG CAGATTACAG ATCTGCTTGA TGTTGTACTA
    GACAGTTTCA TCAAAACCAG TGCAACAGGT GGCTTGGGAT CAATAAAAGC TGAGGTGATG
    GCAGATACTG CTGTAGCTTT GGCTTCTGGA AATGTGAAAT TGGTTTCAAG CAAGGTTATT
    GGAAGGATGT GCAAAATAAT TGACAAGACA TGCTTATCTC CAACTCCTAC TTTAGAACAA
    CATCTTATGT GGGATGATAT TGCTATTTTA GCACGCTACA TGCTGATGCT GTCCTTCAAC
    AATTCCCTTG ATGTGGCAGC TCATCTTCCC TACCTCTTCC ACGTTGTTAC TTTCTTAGTA
    GCCACAGGTC CGCTCTCCCT TAGAGCTTCC ACACATGGAC TGGTCATTAA TATCATTCAC
    TCTCTGTGTA CTTGTTCACA GCTTCATTTT AGTGAAGAGA CCAAGCAAGT TTTGAGACTC
    AGTCTGACAG AGTTCTCATT ACCCAAATTT TACTTGCTGT TTGGCATTAG CAAAGTCAAG
    TCAGCTGCTG TCATTGCCTT CCGTTCCAGT TACCGGGACA GGTCATTCTC TCCTGGCTCC
    TATGAGAGAG AGACTTTTGC TTTGACATCC TTGGAAACAG TCACAGAAGC TTTGTTGGAG
    ATCATGGAGG CATGCATGAG AGATATTCCA ACGTGCAAGT GGCTGGACCA GTGGACAGAA
    CTAGCTCAAA GATTTGCATT CCAATATAAT CCATCCCTGC AACCAAGAGC TCTTGTTGTC
    TTTGGGTGTA TTAGCAAACG AGTGTCTCAT GGGCAGATAA AGCAGATAAT CCGTATTCTT
    AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA CCTGACACTT ACAACAGTCA AGTTCTGATA
    GAAGCTACAG TAATAGCACT AACCAAATTA CAGCCACTTC TTAATAAGGA CTCGCCTCTG
    CACAAAGCCC TCTTTTGGGT AGCTGTGGCT GTGCTGCAGC TTGATGAGGT CAACTTGTAT
    TCAGCAGGTA CCGCACTTCT TGAACAAAAC CTGCATACTT TAGATAGTCT CCGTATATTC
    AATGACAAGA GTCCAGAGGA AGTATTTATG GCAATCCGGA ATCCTCTGGA GTGGCACTGC
    AAGCAAATGG ATCATTTTGT TGGACTCAAT TTCAACTCTA ACTTTAACTT TGCATTGGTT
    GGACACCTTT TAAAAGGGTA CAGGCATCCT TCACCTGCTA TTGTTGCAAG AACAGTCAGA
    ATTTTACATA CACTACTAAC TCTGGTTAAC AAACACAGAA ATTGTGACAA ATTTGAAGTG
    AATACACAGA GCGTGGCCTA CTTAGCAGCT TTACTTACAG TGTCTGAAGA AGTTCGAAGT
    CGCTGCAGCC TAAAACATAG AAAGTCACTT CTTCTTACTG ATATTTCAAT GGAAAATGTT
    CCTATGGATA CATATCCCAT TCATCATGGT GACCCTTCCT ATAGGACACT AAAGGAGACT
    CAGCCATGGT CCTCTCCCAA AGGTTCTGAA GGATACCTTG CAGCCACCTA TCCAACTGTC
    GGCCAGACCA GTCCCCGAGC CAGGAAATCC ATGAGCCTGG ACATGGGGCA ACCTTCTCAG
    GCCAACACTA AGAAGTTGCT TGGAACAAGG AAAAGTTTTG ATCACTTGAT ATCAGACACA
    AAGGCTCCTA AAAGGCAAGA AATGGAATCA GGGATCACAA CACCCCCCAA AATGAGGAGA
    GTAGCAGAAA CTGATTATGA AATGGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
    CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
    GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
    GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
    AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
    TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
    GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
    TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
    GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
    GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
    ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
    CCAGCTTCTC TGCCTTGCTC TAAATCAGCA GTTTTCATGC AGCTGTTCCC TCATCAAGGA
    ATCGACAAGG AGAACGTTGA ACTCTCCCCT ACCACTGGCC ACTGTAACAG TGGACGAACT
    CGCCACGGAT CCGCAAGCCA AGTGCAGAAG CAAAGAAGCG CTGGCAGTTT CAAACGTAAT
    AGCATTAAGA AGATCGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59246
    Clone ID Related Accession (Same CDS sequence) XM_011524857.2
    Accession Version XM_011524857.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8481bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurofibromin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1621
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    1801
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    1981
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    7921
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    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
    CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
    CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
    AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
    AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
    GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
    CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
    GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
    CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
    GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
    AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
    CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
    TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
    AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
    TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
    AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
    TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
    TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
    ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
    AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
    ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
    ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
    TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
    CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
    TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
    CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
    ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
    ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
    CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
    GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
    TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
    CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
    ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
    CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
    AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
    GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
    CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
    CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
    AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
    TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
    GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
    GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
    GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
    CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
    ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
    CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
    TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
    TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
    TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
    GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
    AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
    GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
    ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
    GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
    CAGGCCAACA CTAAGAAGTT GCTTGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
    CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
    GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
    GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
    AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
    TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
    GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
    TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
    GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
    GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
    ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
    CCAGCTTCTC TGCCTTGCTC TAAATCAGCA GTTTTCATGC AGCTGTTCCC TCATCAAGGA
    ATCGACAAGG AGAACGTTGA ACTCTCCCCT ACCACTGGCC ACTGTAACAG TGGACGAACT
    CGCCACGGAT CCGCAAGCCA AGTGCAGAAG CAAAGAAGCG CTGGCAGTTT CAAACGTAAT
    AGCATTAAGA AGATCGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011523159.1
    CDS334..8814
    Translation

    Target ORF information:

    RefSeq Version XM_011524857.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011524857.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    GCAACTTGCC ACTCCCTACT GAATAAAGCT ACAGTAAAAG AAAAAAAGGA AAACAAAAAA
    TCAGTGGTTA GCCAGCGTTT CCCTCAGAAC AGCATCGGTG CAGTAGGAAG TGCCATGTTC
    CTCAGATTTA TCAATCCTGC CATTGTCTCA CCGTATGAAG CAGGGATTTT AGATAAAAAG
    CCACCACCTA GAATCGAAAG GGGCTTGAAG TTAATGTCAA AGATACTTCA GAGTATTGCC
    AATCATGTTC TCTTCACAAA AGAAGAACAT ATGCGGCCTT TCAATGATTT TGTGAAAAGC
    AACTTTGATG CAGCACGCAG GTTTTTCCTT GATATAGCAT CTGATTGTCC TACAAGTGAT
    GCAGTAAATC ATAGTCTTTC CTTCATAAGT GACGGCAATG TGCTTGCTTT ACATCGTCTA
    CTCTGGAACA ATCAGGAGAA AATTGGGCAG TATCTTTCCA GCAACAGGGA TCATAAAGCT
    GTTGGAAGAC GACCTTTTGA TAAGATGGCA ACACTTCTTG CATACCTGGG TCCTCCAGAG
    CACAAACCTG TGGCAGATAC ACACTGGTCC AGCCTTAACC TTACCAGTTC AAAGTTTGAG
    GAATTTATGA CTAGGCATCA GGTACATGAA AAAGAAGAAT TCAAGGCTTT GAAAACGTTA
    AGTATTTTCT ACCAAGCTGG GACTTCCAAA GCTGGGAATC CTATTTTTTA TTATGTTGCA
    CGGAGGTTCA AAACTGGTCA AATCAATGGT GATTTGCTGA TATACCATGT CTTACTGACT
    TTAAAGCCAT ATTATGCAAA GCCATATGAA ATTGTAGTGG ACCTTACCCA TACCGGGCCT
    AGCAATCGCT TTAAAACAGA CTTTCTCTCT AAGTGGTTTG TTGTTTTTCC TGGCTTTGCT
    TACGACAACG TCTCCGCAGT CTATATCTAT AACTGTAACT CCTGGGTCAG GGAGTACACC
    AAGTATCATG AGCGGCTGCT GACTGGCCTC AAAGGTAGCA AAAGGCTTGT TTTCATAGAC
    TGTCCTGGGA AACTGGCTGA GCACATAGAG CATGAACAAC AGAAACTACC TGCTGCCACC
    TTGGCTTTAG AAGAGGACCT GAAGGTATTC CACAATGCTC TCAAGCTAGC TCACAAAGAC
    ACCAAAGTTT CTATTAAAGT TGGTTCTACT GCTGTCCAAG TAACTTCAGC AGAGCGAACA
    AAAGTCCTAG GGCAATCAGT CTTTCTAAAT GACATTTATT ATGCTTCGGA AATTGAAGAA
    ATCTGCCTAG TAGATGAGAA CCAGTTCACC TTAACCATTG CAAACCAGGG CACGCCGCTC
    ACCTTCATGC ACCAGGAGTG TGAAGCCATT GTCCAGTCTA TCATTCATAT CCGGACCCGC
    TGGGAACTGT CACAGCCCGA CTCTATCCCC CAACACACCA AGATTCGGCC AAAAGATGTC
    CCTGGGACAC TGCTCAATAT CGCATTACTT AATTTAGGCA GTTCTGACCC GAGTTTACGG
    TCAGCTGCCT ATAATCTTCT GTGTGCCTTA ACTTGTACCT TTAATTTAAA AATCGAGGGC
    CAGTTACTAG AGACATCAGG TTTATGTATC CCTGCCAACA ACACCCTCTT TATTGTCTCT
    ATTAGTAAGA CACTGGCAGC CAATGAGCCA CACCTCACGT TAGAATTTTT GGAAGAGTGT
    ATTTCTGGAT TTAGCAAATC TAGTATTGAA TTGAAACACC TTTGTTTGGA ATACATGACT
    CCATGGCTGT CAAATCTAGT TCGTTTTTGC AAGCATAATG ATGATGCCAA ACGACAAAGA
    GTTACTGCTA TTCTTGACAA GCTGATAACA ATGACCATCA ATGAAAAACA GATGTACCCA
    TCTATTCAAG CAAAAATATG GGGAAGCCTT GGGCAGATTA CAGATCTGCT TGATGTTGTA
    CTAGACAGTT TCATCAAAAC CAGTGCAACA GGTGGCTTGG GATCAATAAA AGCTGAGGTG
    ATGGCAGATA CTGCTGTAGC TTTGGCTTCT GGAAATGTGA AATTGGTTTC AAGCAAGGTT
    ATTGGAAGGA TGTGCAAAAT AATTGACAAG ACATGCTTAT CTCCAACTCC TACTTTAGAA
    CAACATCTTA TGTGGGATGA TATTGCTATT TTAGCACGCT ACATGCTGAT GCTGTCCTTC
    AACAATTCCC TTGATGTGGC AGCTCATCTT CCCTACCTCT TCCACGTTGT TACTTTCTTA
    GTAGCCACAG GTCCGCTCTC CCTTAGAGCT TCCACACATG GACTGGTCAT TAATATCATT
    CACTCTCTGT GTACTTGTTC ACAGCTTCAT TTTAGTGAAG AGACCAAGCA AGTTTTGAGA
    CTCAGTCTGA CAGAGTTCTC ATTACCCAAA TTTTACTTGC TGTTTGGCAT TAGCAAAGTC
    AAGTCAGCTG CTGTCATTGC CTTCCGTTCC AGTTACCGGG ACAGGTCATT CTCTCCTGGC
    TCCTATGAGA GAGAGACTTT TGCTTTGACA TCCTTGGAAA CAGTCACAGA AGCTTTGTTG
    GAGATCATGG AGGCATGCAT GAGAGATATT CCAACGTGCA AGTGGCTGGA CCAGTGGACA
    GAACTAGCTC AAAGATTTGC ATTCCAATAT AATCCATCCC TGCAACCAAG AGCTCTTGTT
    GTCTTTGGGT GTATTAGCAA ACGAGTGTCT CATGGGCAGA TAAAGCAGAT AATCCGTATT
    CTTAGCAAGG CACTTGAGAG TTGCTTAAAA GGACCTGACA CTTACAACAG TCAAGTTCTG
    ATAGAAGCTA CAGTAATAGC ACTAACCAAA TTACAGCCAC TTCTTAATAA GGACTCGCCT
    CTGCACAAAG CCCTCTTTTG GGTAGCTGTG GCTGTGCTGC AGCTTGATGA GGTCAACTTG
    TATTCAGCAG GTACCGCACT TCTTGAACAA AACCTGCATA CTTTAGATAG TCTCCGTATA
    TTCAATGACA AGAGTCCAGA GGAAGTATTT ATGGCAATCC GGAATCCTCT GGAGTGGCAC
    TGCAAGCAAA TGGATCATTT TGTTGGACTC AATTTCAACT CTAACTTTAA CTTTGCATTG
    GTTGGACACC TTTTAAAAGG GTACAGGCAT CCTTCACCTG CTATTGTTGC AAGAACAGTC
    AGAATTTTAC ATACACTACT AACTCTGGTT AACAAACACA GAAATTGTGA CAAATTTGAA
    GTGAATACAC AGAGCGTGGC CTACTTAGCA GCTTTACTTA CAGTGTCTGA AGAAGTTCGA
    AGTCGCTGCA GCCTAAAACA TAGAAAGTCA CTTCTTCTTA CTGATATTTC AATGGAAAAT
    GTTCCTATGG ATACATATCC CATTCATCAT GGTGACCCTT CCTATAGGAC ACTAAAGGAG
    ACTCAGCCAT GGTCCTCTCC CAAAGGTTCT GAAGGATACC TTGCAGCCAC CTATCCAACT
    GTCGGCCAGA CCAGTCCCCG AGCCAGGAAA TCCATGAGCC TGGACATGGG GCAACCTTCT
    CAGGCCAACA CTAAGAAGTT GCTTGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
    CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
    GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
    GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
    AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
    TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
    GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
    TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
    GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
    GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
    ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
    CCAGCTTCTC TGCCTTGCTC TAAATCAGCA GTTTTCATGC AGCTGTTCCC TCATCAAGGA
    ATCGACAAGG AGAACGTTGA ACTCTCCCCT ACCACTGGCC ACTGTAACAG TGGACGAACT
    CGCCACGGAT CCGCAAGCCA AGTGCAGAAG CAAAGAAGCG CTGGCAGTTT CAAACGTAAT
    AGCATTAAGA AGATCGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86894
    Clone ID Related Accession (Same CDS sequence) XM_017024689.1
    Accession Version XM_017024689.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurofibromin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    GTGGTTAGCC AGCGTTTCCC TCAGAACAGC ATCGGTGCAG TAGGAAGTGC CATGTTCCTC
    AGATTTATCA ATCCTGCCAT TGTCTCACCG TATGAAGCAG GGATTTTAGA TAAAAAGCCA
    CCACCTAGAA TCGAAAGGGG CTTGAAGTTA ATGTCAAAGA TACTTCAGAG TATTGCCAAT
    CATGTTCTCT TCACAAAAGA AGAACATATG CGGCCTTTCA ATGATTTTGT GAAAAGCAAC
    TTTGATGCAG CACGCAGGTT TTTCCTTGAT ATAGCATCTG ATTGTCCTAC AAGTGATGCA
    GTAAATCATA GTCTTTCCTT CATAAGTGAC GGCAATGTGC TTGCTTTACA TCGTCTACTC
    TGGAACAATC AGGAGAAAAT TGGGCAGTAT CTTTCCAGCA ACAGGGATCA TAAAGCTGTT
    GGAAGACGAC CTTTTGATAA GATGGCAACA CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC
    AAACCTGTGG CAGATACACA CTGGTCCAGC CTTAACCTTA CCAGTTCAAA GTTTGAGGAA
    TTTATGACTA GGCATCAGGT ACATGAAAAA GAAGAATTCA AGGCTTTGAA AACGTTAAGT
    ATTTTCTACC AAGCTGGGAC TTCCAAAGCT GGGAATCCTA TTTTTTATTA TGTTGCACGG
    AGGTTCAAAA CTGGTCAAAT CAATGGTGAT TTGCTGATAT ACCATGTCTT ACTGACTTTA
    AAGCCATATT ATGCAAAGCC ATATGAAATT GTAGTGGACC TTACCCATAC CGGGCCTAGC
    AATCGCTTTA AAACAGACTT TCTCTCTAAG TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC
    GACAACGTCT CCGCAGTCTA TATCTATAAC TGTAACTCCT GGGTCAGGGA GTACACCAAG
    TATCATGAGC GGCTGCTGAC TGGCCTCAAA GGTAGCAAAA GGCTTGTTTT CATAGACTGT
    CCTGGGAAAC TGGCTGAGCA CATAGAGCAT GAACAACAGA AACTACCTGC TGCCACCTTG
    GCTTTAGAAG AGGACCTGAA GGTATTCCAC AATGCTCTCA AGCTAGCTCA CAAAGACACC
    AAAGTTTCTA TTAAAGTTGG TTCTACTGCT GTCCAAGTAA CTTCAGCAGA GCGAACAAAA
    GTCCTAGGGC AATCAGTCTT TCTAAATGAC ATTTATTATG CTTCGGAAAT TGAAGAAATC
    TGCCTAGTAG ATGAGAACCA GTTCACCTTA ACCATTGCAA ACCAGGGCAC GCCGCTCACC
    TTCATGCACC AGGAGTGTGA AGCCATTGTC CAGTCTATCA TTCATATCCG GACCCGCTGG
    GAACTGTCAC AGCCCGACTC TATCCCCCAA CACACCAAGA TTCGGCCAAA AGATGTCCCT
    GGGACACTGC TCAATATCGC ATTACTTAAT TTAGGCAGTT CTGACCCGAG TTTACGGTCA
    GCTGCCTATA ATCTTCTGTG TGCCTTAACT TGTACCTTTA ATTTAAAAAT CGAGGGCCAG
    TTACTAGAGA CATCAGGTTT ATGTATCCCT GCCAACAACA CCCTCTTTAT TGTCTCTATT
    AGTAAGACAC TGGCAGCCAA TGAGCCACAC CTCACGTTAG AATTTTTGGA AGAGTGTATT
    TCTGGATTTA GCAAATCTAG TATTGAATTG AAACACCTTT GTTTGGAATA CATGACTCCA
    TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG CATAATGATG ATGCCAAACG ACAAAGAGTT
    ACTGCTATTC TTGACAAGCT GATAACAATG ACCATCAATG AAAAACAGAT GTACCCATCT
    ATTCAAGCAA AAATATGGGG AAGCCTTGGG CAGATTACAG ATCTGCTTGA TGTTGTACTA
    GACAGTTTCA TCAAAACCAG TGCAACAGGT GGCTTGGGAT CAATAAAAGC TGAGGTGATG
    GCAGATACTG CTGTAGCTTT GGCTTCTGGA AATGTGAAAT TGGTTTCAAG CAAGGTTATT
    GGAAGGATGT GCAAAATAAT TGACAAGACA TGCTTATCTC CAACTCCTAC TTTAGAACAA
    CATCTTATGT GGGATGATAT TGCTATTTTA GCACGCTACA TGCTGATGCT GTCCTTCAAC
    AATTCCCTTG ATGTGGCAGC TCATCTTCCC TACCTCTTCC ACGTTGTTAC TTTCTTAGTA
    GCCACAGGTC CGCTCTCCCT TAGAGCTTCC ACACATGGAC TGGTCATTAA TATCATTCAC
    TCTCTGTGTA CTTGTTCACA GCTTCATTTT AGTGAAGAGA CCAAGCAAGT TTTGAGACTC
    AGTCTGACAG AGTTCTCATT ACCCAAATTT TACTTGCTGT TTGGCATTAG CAAAGTCAAG
    TCAGCTGCTG TCATTGCCTT CCGTTCCAGT TACCGGGACA GGTCATTCTC TCCTGGCTCC
    TATGAGAGAG AGACTTTTGC TTTGACATCC TTGGAAACAG TCACAGAAGC TTTGTTGGAG
    ATCATGGAGG CATGCATGAG AGATATTCCA ACGTGCAAGT GGCTGGACCA GTGGACAGAA
    CTAGCTCAAA GATTTGCATT CCAATATAAT CCATCCCTGC AACCAAGAGC TCTTGTTGTC
    TTTGGGTGTA TTAGCAAACG AGTGTCTCAT GGGCAGATAA AGCAGATAAT CCGTATTCTT
    AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA CCTGACACTT ACAACAGTCA AGTTCTGATA
    GAAGCTACAG TAATAGCACT AACCAAATTA CAGCCACTTC TTAATAAGGA CTCGCCTCTG
    CACAAAGCCC TCTTTTGGGT AGCTGTGGCT GTGCTGCAGC TTGATGAGGT CAACTTGTAT
    TCAGCAGGTA CCGCACTTCT TGAACAAAAC CTGCATACTT TAGATAGTCT CCGTATATTC
    AATGACAAGA GTCCAGAGGA AGTATTTATG GCAATCCGGA ATCCTCTGGA GTGGCACTGC
    AAGCAAATGG ATCATTTTGT TGGACTCAAT TTCAACTCTA ACTTTAACTT TGCATTGGTT
    GGACACCTTT TAAAAGGGTA CAGGCATCCT TCACCTGCTA TTGTTGCAAG AACAGTCAGA
    ATTTTACATA CACTACTAAC TCTGGTTAAC AAACACAGAA ATTGTGACAA ATTTGAAGTG
    AATACACAGA GCGTGGCCTA CTTAGCAGCT TTACTTACAG TGTCTGAAGA AGTTCGAAGT
    CGCTGCAGCC TAAAACATAG AAAGTCACTT CTTCTTACTG ATATTTCAAT GGAAAATGTT
    CCTATGGATA CATATCCCAT TCATCATGGT GACCCTTCCT ATAGGACACT AAAGGAGACT
    CAGCCATGGT CCTCTCCCAA AGGTTCTGAA GGATACCTTG CAGCCACCTA TCCAACTGTC
    GGCCAGACCA GTCCCCGAGC CAGGAAATCC ATGAGCCTGG ACATGGGGCA ACCTTCTCAG
    GCCAACACTA AGAAGTTGCT TGGAACAAGG AAAAGTTTTG ATCACTTGAT ATCAGACACA
    AAGGCTCCTA AAAGGCAAGA AATGGAATCA GGGATCACAA CACCCCCCAA AATGAGGAGA
    GTAGCAGAAA CTGATTATGA AATGGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
    CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
    GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
    GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
    AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
    TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
    GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
    TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
    GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
    GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
    ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
    CCAGGAATCG ACAAGGAGAA CGTTGAACTC TCCCCTACCA CTGGCCACTG TAACAGTGGA
    CGAACTCGCC ACGGATCCGC AAGCCAAGTG CAGAAGCAAA GAAGCGCTGG CAGTTTCAAA
    CGTAATAGCA TTAAGAAGAT CGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880178.1
    CDS334..8820
    Translation

    Target ORF information:

    RefSeq Version XM_017024689.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neurofibromin 1 (NF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024689.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCCGCGC ACAGGCCGGT GGAATGGGTC CAGGCCGTGG TCAGCCGCTT CGACGAGCAG 
    CTTCCAATAA AAACAGGACA GCAGAACACA CATACCAAAG TCAGTACTGA GCACAACAAG
    GAATGTCTAA TCAATATTTC CAAATACAAG TTTTCTTTGG TTATAAGCGG CCTCACTACT
    ATTTTAAAGA ATGTTAACAA TATGAGAATA TTTGGAGAAG CTGCTGAAAA AAATTTATAT
    CTCTCTCAGT TGATTATATT GGATACACTG GAAAAATGTC TTGCTGGGCA ACCAAAGGAC
    ACAATGAGAT TAGATGAAAC GATGCTGGTC AAACAGTTGC TGCCAGAAAT CTGCCATTTT
    CTTCACACCT GTCGTGAAGG AAACCAGCAT GCAGCTGAAC TTCGGAATTC TGCCTCTGGG
    GTTTTATTTT CTCTCAGCTG CAACAACTTC AATGCAGTCT TTAGTCGCAT TTCTACCAGG
    TTACAGGAAT TAACTGTTTG TTCAGAAGAC AATGTTGATG TTCATGATAT AGAATTGTTA
    CAGTATATCA ATGTGGATTG TGCAAAATTA AAACGACTCC TGAAGGAAAC AGCATTTAAA
    TTTAAAGCCC TAAAGAAGGT TGCGCAGTTA GCAGTTATAA ATAGCCTGGA AAAGGCATTT
    TGGAACTGGG TAGAAAATTA TCCAGATGAA TTTACAAAAC TGTACCAGAT CCCACAGACT
    GATATGGCTG AATGTGCAGA AAAGCTATTT GACTTGGTGG ATGGTTTTGC TGAAAGCACC
    AAACGTAAAG CAGCAGTTTG GCCACTACAA ATCATTCTCC TTATCTTGTG TCCAGAAATA
    ATCCAGGATA TATCCAAAGA CGTGGTTGAT GAAAACAACA TGAATAAGAA GTTATTTCTG
    GACAGTCTAC GAAAAGCTCT TGCTGGCCAT GGAGGAAGTA GGCAGCTGAC AGAAAGTGCT
    GCAATTGCCT GTGTCAAACT GTGTAAAGCA AGTACTTACA TCAATTGGGA AGATAACTCT
    GTCATTTTCC TACTTGTTCA GTCCATGGTG GTTGATCTTA AGAACCTGCT TTTTAATCCA
    AGTAAGCCAT TCTCAAGAGG CAGTCAGCCT GCAGATGTGG ATCTAATGAT TGACTGCCTT
    GTTTCTTGCT TTCGTATAAG CCCTCACAAC AACCAACACT TTAAGATCTG CCTGGCTCAG
    AATTCACCTT CTACATTTCA CTATGTGCTG GTAAATTCAC TCCATCGAAT CATCACCAAT
    AGCACTTTCA AGCATGGACT TGGCACTGCT TCCGCATTGG ATTGGTGGCC TAAGATTGAT
    GCTGTGTATT GTCACTCGGT TGAACTTCGA AATATGTTTG GTGAAACACT TCATAAAGCA
    GTGCAAGGTT GTGGAGCACA CCCAGCAATA CGAATGGCAC CGAGTCTTAC ATTTAAAGAA
    AAAGTAACAA GCCTTAAATT TAAAGAAAAA CCTACAGACC TGGAGACAAG AAGCTATAAG
    TATCTTCTCT TGTCCATGGT GAAACTAATT CATGCAGATC CAAAGCTCTT GCTTTGTAAT
    CCAAGAAAAC AGGGGCCCGA AACCCAAGGC AGTACAGCAG AATTAATTAC AGGGCTCGTC
    CAACTGGTCC CTCAGTCACA CATGCCAGAG ATTGCTCAGG AAGCAATGGA GGCTCTGCTG
    GTTCTTCATC AGTTAGATAG CATTGATTTG TGGAATCCTG ATGCTCCTGT AGAAACATTT
    TGGGAGATTA GCTCACAAAT GCTTTTTTAC ATCTGCAAGA AATTAACTAG TCATCAAATG
    CTTAGTAGCA CAGAAATTCT CAAGTGGTTG CGGGAAATAT TGATCTGCAG GAATAAATTT
    CTTCTTAAAA ATAAGCAGGC AGATAGAAGT TCCTGTCACT TTCTCCTTTT TTACGGGGTA
    GGATGTGATA TTCCTTCTAG TGGAAATACC AGTCAAATGT CCATGGATCA TGAAGAATTA
    CTACGTACTC CTGGAGCCTC TCTCCGGAAG GGAAAAGGGA ACTCCTCTAT GGATAGTGCA
    GCAGGATGCA GCGGAACCCC CCCGATTTGC CGACAAGCCC AGACCAAACT AGAAGTGGCC
    CTGTACATGT TTCTGTGGAA CCCTGACACT GAAGCTGTTC TGGTTGCCAT GTCCTGTTTC
    CGCCACCTCT GTGAGGAAGC AGATATCCGG TGTGGGGTGG ATGAAGTGTC AGTGCATAAC
    CTCTTGCCCA ACTATAACAC ATTCATGGAG TTTGCCTCTG TCAGCAATAT GATGTCAACA
    GGAAGAGCAG CACTTCAGAA AAGAGTGATG GCACTGCTGA GGCGCATTGA GCATCCCACT
    GCAGGAAACA CTGAGGCTTG GGAAGATACA CATGCAAAAT GGGAACAAGC AACAAAGCTA
    ATCCTTAACT ATCCAAAAGC CAAAATGGAA GATGGCCAGG CTGCTGAAAG CCTTCACAAG
    ACCATTGTTA AGAGGCGAAT GTCCCATGTG AGTGGAGGAG GATCCATAGA TTTGTCTGAC
    ACAGACTCCC TACAGGAATG GATCAACATG ACTGGCTTCC TTTGTGCCCT TGGGGGAGTG
    TGCCTCCAGC AGAGAAGCAA TTCTGGCCTG GCAACCTATA GCCCACCCAT GGGTCCAGTC
    AGTGAACGTA AGGGTTCTAT GATTTCAGTG ATGTCTTCAG AGGGAAACGC AGATACACCT
    GTCAGCAAAT TTATGGATCG GCTGTTGTCC TTAATGGTGT GTAACCATGA GAAAGTGGGA
    CTTCAAATAC GGACCAATGT TAAGGATCTG GTGGGTCTAG AATTGAGTCC TGCTCTGTAT
    CCAATGCTAT TTAACAAATT GAAGAATACC ATCAGCAAGT TTTTTGACTC CCAAGGACAG
    GTTTTATTGA CTGATACCAA TACTCAATTT GTAGAACAAA CCATAGCTAT AATGAAGAAC
    TTGCTAGATA ATCATACTGA AGGCAGCTCT GAACATCTAG GGCAAGCTAG CATTGAAACA
    ATGATGTTAA ATCTGGTCAG GTATGTTCGT GTGCTTGGGA ATATGGTCCA TGCAATTCAA
    ATAAAAACGA AACTGTGTCA ATTAGTTGAA GTAATGATGG CAAGGAGAGA TGACCTCTCA
    TTTTGCCAAG AGATGAAATT TAGGAATAAG ATGGTAGAAT ACCTGACAGA CTGGGTTATG
    GGAACATCAA ACCAAGCAGC AGATGATGAT GTAAAATGTC TTACAAGAGA TTTGGACCAG
    GCAAGCATGG AAGCAGTAGT TTCACTTCTA GCTGGTCTCC CTCTGCAGCC TGAAGAAGGA
    GATGGTGTGG AATTGATGGA AGCCAAATCA CAGTTATTTC TTAAATACTT CACATTATTT
    ATGAACCTTT TGAATGACTG CAGTGAAGTT GAAGATGAAA GTGCGCAAAC AGGTGGCAGG
    AAACGTGGCA TGTCTCGGAG GCTGGCATCA CTGAGGCACT GTACGGTCCT TGCAATGTCA
    AACTTACTCA ATGCCAACGT AGACAGTGGT CTCATGCACT CCATAGGCTT AGGTTACCAC
    AAGGATCTCC AGACAAGAGC TACATTTATG GAAGTTCTGA CAAAAATCCT TCAACAAGGC
    ACAGAATTTG ACACACTTGC AGAAACAGTA TTGGCTGATC GGTTTGAGAG ATTGGTGGAA
    CTGGTCACAA TGATGGGTGA TCAAGGAGAA CTCCCTATAG CGATGGCTCT GGCCAATGTG
    GTTCCTTGTT CTCAGTGGGA TGAACTAGCT CGAGTTCTGG TTACTCTGTT TGATTCTCGG
    CATTTACTCT ACCAACTGCT CTGGAACATG TTTTCTAAAG AAGTAGAATT GGCAGACTCC
    ATGCAGACTC TCTTCCGAGG CAACAGCTTG GCCAGTAAAA TAATGACATT CTGTTTCAAG
    GTATATGGTG CTACCTATCT ACAAAAACTC CTGGATCCTT TATTACGAAT TGTGATCACA
    TCCTCTGATT GGCAACATGT TAGCTTTGAA GTGGATCCTA CCAGGTTAGA ACCATCAGAG
    AGCCTTGAGG AAAACCAGCG GAACCTCCTT CAGATGACTG AAAAGTTCTT CCATGCCATC
    ATCAGTTCCT CCTCAGAATT CCCCCCTCAA CTTCGAAGTG TGTGCCACTG TTTATACCAG
    GTGGTTAGCC AGCGTTTCCC TCAGAACAGC ATCGGTGCAG TAGGAAGTGC CATGTTCCTC
    AGATTTATCA ATCCTGCCAT TGTCTCACCG TATGAAGCAG GGATTTTAGA TAAAAAGCCA
    CCACCTAGAA TCGAAAGGGG CTTGAAGTTA ATGTCAAAGA TACTTCAGAG TATTGCCAAT
    CATGTTCTCT TCACAAAAGA AGAACATATG CGGCCTTTCA ATGATTTTGT GAAAAGCAAC
    TTTGATGCAG CACGCAGGTT TTTCCTTGAT ATAGCATCTG ATTGTCCTAC AAGTGATGCA
    GTAAATCATA GTCTTTCCTT CATAAGTGAC GGCAATGTGC TTGCTTTACA TCGTCTACTC
    TGGAACAATC AGGAGAAAAT TGGGCAGTAT CTTTCCAGCA ACAGGGATCA TAAAGCTGTT
    GGAAGACGAC CTTTTGATAA GATGGCAACA CTTCTTGCAT ACCTGGGTCC TCCAGAGCAC
    AAACCTGTGG CAGATACACA CTGGTCCAGC CTTAACCTTA CCAGTTCAAA GTTTGAGGAA
    TTTATGACTA GGCATCAGGT ACATGAAAAA GAAGAATTCA AGGCTTTGAA AACGTTAAGT
    ATTTTCTACC AAGCTGGGAC TTCCAAAGCT GGGAATCCTA TTTTTTATTA TGTTGCACGG
    AGGTTCAAAA CTGGTCAAAT CAATGGTGAT TTGCTGATAT ACCATGTCTT ACTGACTTTA
    AAGCCATATT ATGCAAAGCC ATATGAAATT GTAGTGGACC TTACCCATAC CGGGCCTAGC
    AATCGCTTTA AAACAGACTT TCTCTCTAAG TGGTTTGTTG TTTTTCCTGG CTTTGCTTAC
    GACAACGTCT CCGCAGTCTA TATCTATAAC TGTAACTCCT GGGTCAGGGA GTACACCAAG
    TATCATGAGC GGCTGCTGAC TGGCCTCAAA GGTAGCAAAA GGCTTGTTTT CATAGACTGT
    CCTGGGAAAC TGGCTGAGCA CATAGAGCAT GAACAACAGA AACTACCTGC TGCCACCTTG
    GCTTTAGAAG AGGACCTGAA GGTATTCCAC AATGCTCTCA AGCTAGCTCA CAAAGACACC
    AAAGTTTCTA TTAAAGTTGG TTCTACTGCT GTCCAAGTAA CTTCAGCAGA GCGAACAAAA
    GTCCTAGGGC AATCAGTCTT TCTAAATGAC ATTTATTATG CTTCGGAAAT TGAAGAAATC
    TGCCTAGTAG ATGAGAACCA GTTCACCTTA ACCATTGCAA ACCAGGGCAC GCCGCTCACC
    TTCATGCACC AGGAGTGTGA AGCCATTGTC CAGTCTATCA TTCATATCCG GACCCGCTGG
    GAACTGTCAC AGCCCGACTC TATCCCCCAA CACACCAAGA TTCGGCCAAA AGATGTCCCT
    GGGACACTGC TCAATATCGC ATTACTTAAT TTAGGCAGTT CTGACCCGAG TTTACGGTCA
    GCTGCCTATA ATCTTCTGTG TGCCTTAACT TGTACCTTTA ATTTAAAAAT CGAGGGCCAG
    TTACTAGAGA CATCAGGTTT ATGTATCCCT GCCAACAACA CCCTCTTTAT TGTCTCTATT
    AGTAAGACAC TGGCAGCCAA TGAGCCACAC CTCACGTTAG AATTTTTGGA AGAGTGTATT
    TCTGGATTTA GCAAATCTAG TATTGAATTG AAACACCTTT GTTTGGAATA CATGACTCCA
    TGGCTGTCAA ATCTAGTTCG TTTTTGCAAG CATAATGATG ATGCCAAACG ACAAAGAGTT
    ACTGCTATTC TTGACAAGCT GATAACAATG ACCATCAATG AAAAACAGAT GTACCCATCT
    ATTCAAGCAA AAATATGGGG AAGCCTTGGG CAGATTACAG ATCTGCTTGA TGTTGTACTA
    GACAGTTTCA TCAAAACCAG TGCAACAGGT GGCTTGGGAT CAATAAAAGC TGAGGTGATG
    GCAGATACTG CTGTAGCTTT GGCTTCTGGA AATGTGAAAT TGGTTTCAAG CAAGGTTATT
    GGAAGGATGT GCAAAATAAT TGACAAGACA TGCTTATCTC CAACTCCTAC TTTAGAACAA
    CATCTTATGT GGGATGATAT TGCTATTTTA GCACGCTACA TGCTGATGCT GTCCTTCAAC
    AATTCCCTTG ATGTGGCAGC TCATCTTCCC TACCTCTTCC ACGTTGTTAC TTTCTTAGTA
    GCCACAGGTC CGCTCTCCCT TAGAGCTTCC ACACATGGAC TGGTCATTAA TATCATTCAC
    TCTCTGTGTA CTTGTTCACA GCTTCATTTT AGTGAAGAGA CCAAGCAAGT TTTGAGACTC
    AGTCTGACAG AGTTCTCATT ACCCAAATTT TACTTGCTGT TTGGCATTAG CAAAGTCAAG
    TCAGCTGCTG TCATTGCCTT CCGTTCCAGT TACCGGGACA GGTCATTCTC TCCTGGCTCC
    TATGAGAGAG AGACTTTTGC TTTGACATCC TTGGAAACAG TCACAGAAGC TTTGTTGGAG
    ATCATGGAGG CATGCATGAG AGATATTCCA ACGTGCAAGT GGCTGGACCA GTGGACAGAA
    CTAGCTCAAA GATTTGCATT CCAATATAAT CCATCCCTGC AACCAAGAGC TCTTGTTGTC
    TTTGGGTGTA TTAGCAAACG AGTGTCTCAT GGGCAGATAA AGCAGATAAT CCGTATTCTT
    AGCAAGGCAC TTGAGAGTTG CTTAAAAGGA CCTGACACTT ACAACAGTCA AGTTCTGATA
    GAAGCTACAG TAATAGCACT AACCAAATTA CAGCCACTTC TTAATAAGGA CTCGCCTCTG
    CACAAAGCCC TCTTTTGGGT AGCTGTGGCT GTGCTGCAGC TTGATGAGGT CAACTTGTAT
    TCAGCAGGTA CCGCACTTCT TGAACAAAAC CTGCATACTT TAGATAGTCT CCGTATATTC
    AATGACAAGA GTCCAGAGGA AGTATTTATG GCAATCCGGA ATCCTCTGGA GTGGCACTGC
    AAGCAAATGG ATCATTTTGT TGGACTCAAT TTCAACTCTA ACTTTAACTT TGCATTGGTT
    GGACACCTTT TAAAAGGGTA CAGGCATCCT TCACCTGCTA TTGTTGCAAG AACAGTCAGA
    ATTTTACATA CACTACTAAC TCTGGTTAAC AAACACAGAA ATTGTGACAA ATTTGAAGTG
    AATACACAGA GCGTGGCCTA CTTAGCAGCT TTACTTACAG TGTCTGAAGA AGTTCGAAGT
    CGCTGCAGCC TAAAACATAG AAAGTCACTT CTTCTTACTG ATATTTCAAT GGAAAATGTT
    CCTATGGATA CATATCCCAT TCATCATGGT GACCCTTCCT ATAGGACACT AAAGGAGACT
    CAGCCATGGT CCTCTCCCAA AGGTTCTGAA GGATACCTTG CAGCCACCTA TCCAACTGTC
    GGCCAGACCA GTCCCCGAGC CAGGAAATCC ATGAGCCTGG ACATGGGGCA ACCTTCTCAG
    GCCAACACTA AGAAGTTGCT TGGAACAAGG AAAAGTTTTG ATCACTTGAT ATCAGACACA
    AAGGCTCCTA AAAGGCAAGA AATGGAATCA GGGATCACAA CACCCCCCAA AATGAGGAGA
    GTAGCAGAAA CTGATTATGA AATGGAAACT CAGAGGATTT CCTCATCACA ACAGCACCCA
    CATTTACGTA AAGTTTCAGT GTCTGAATCA AATGTTCTCT TGGATGAAGA AGTACTTACT
    GATCCGAAGA TCCAGGCGCT GCTTCTTACT GTTCTAGCTA CACTGGTAAA ATATACCACA
    GATGAGTTTG ATCAACGAAT TCTTTATGAA TACTTAGCAG AGGCCAGTGT TGTGTTTCCC
    AAAGTCTTTC CTGTTGTGCA TAATTTGTTG GACTCTAAGA TCAACACCCT GTTATCATTG
    TGCCAAGATC CAAATTTGTT AAATCCAATC CATGGAATTG TGCAGAGTGT GGTGTACCAT
    GAAGAATCCC CACCACAATA CCAAACATCT TACCTGCAAA GTTTTGGTTT TAATGGCTTG
    TGGCGGTTTG CAGGACCGTT TTCAAAGCAA ACACAAATTC CAGACTATGC TGAGCTTATT
    GTTAAGTTTC TTGATGCCTT GATTGACACG TACCTGCCTG GAATTGATGA AGAAACCAGT
    GAAGAATCCC TCCTGACTCC CACATCTCCT TACCCTCCTG CACTGCAGAG CCAGCTTAGT
    ATCACTGCCA ACCTTAACCT TTCTAATTCC ATGACCTCAC TTGCAACTTC CCAGCATTCC
    CCAGGAATCG ACAAGGAGAA CGTTGAACTC TCCCCTACCA CTGGCCACTG TAACAGTGGA
    CGAACTCGCC ACGGATCCGC AAGCCAAGTG CAGAAGCAAA GAAGCGCTGG CAGTTTCAAA
    CGTAATAGCA TTAAGAAGAT CGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.