NEU2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEU2
Entrez Gene ID	4759
Full Name	neuraminidase 2
Synonyms	SIAL2
General protein information	Preferred Names neuraminidase 2 Names sialidase-2 N-acetyl-alpha-neuraminidase 2 cytosolic sialidase sialidase 2 (cytosolic sialidase)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q37.1
Summary	This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq, Jul 2008].
Disorder MIM:	605528

mRNA and Protein(s)

mRNA	Protein	Name
NM_005383.2	NP_005374.2	sialidase-2

Pathways from BioSystems

Homologies

Homo sapiens (human)	NEU2	NP_005374.2
Pan troglodytes (chimpanzee)	NEU2	XP_003309574.1
Canis lupus familiaris (dog)	NEU2	XP_005635919.1
Bos taurus (cattle)	NEU2	XP_003585975.1
Gallus gallus (chicken)	NEU2	XP_004936983.1
Mus musculus (house mouse)	Neu2	NP_001153637.1
Rattus norvegicus (Norway rat)	Neu2	NP_058826.2
	NEU2	XP_001114628.1

Gene Ontology
Bibliography

Related articles in PubMed

Membrane restructuring following in situ sialidase digestion of gangliosides: Complex model bilayers by synchrotron radiation reflectivity.
Rondelli V, Brocca P, Fragneto G, Daillant J, Tringali C, Cantu' L, Del Favero E
Biochimica et biophysica acta1859(5)845-851(2017 05)

Co-Expression of NEU2 and GBA3 Causes a Drastic Reduction in Cytosolic Sialyl Free N-glycans in Human MKN45 Stomach Cancer Cells-Evidence for the Physical Interaction of NEU2 and GBA3.
Wang L, Seino J, Tomotake H, Funakoshi Y, Hirayama H, Suzuki T
Biomolecules5(3)1499-514(2015 Jul)

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Looking at human cytosolic sialidase NEU2 structural features with an interdisciplinary approach.
Monti E, Benaglia G, Mozzi A, Fusi P, Longhi G, Gangemi F, Castiglioni E, Woody RW, Fornili SL, Abbate S
Biochemistry53(32)5343-55(2014 Aug)

Variants in the 1q21 risk region are associated with a visual endophenotype of autism and schizophrenia.
Goodbourn PT, Bosten JM, Bargary G, Hogg RE, Lawrance-Owen AJ, Mollon JD
Genes, brain, and behavior13(2)144-51(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Synchrotron radiation reflectometry was used to access the transverse structure of model membranes under the action of the human sialidase NEU2, down to the Angstrom length scale
Title: Membrane restructuring following in situ sialidase digestion of gangliosides: Complex model bilayers by synchrotron radiation reflectivity.

cytosolic GBA3 is likely involved in the catabolism of cytosolic sialyl free N-glycans, possibly by stabilizing the activity of the NEU2 protein
Title: Co-Expression of NEU2 and GBA3 Causes a Drastic Reduction in Cytosolic Sialyl Free N-glycans in Human MKN45 Stomach Cancer Cells-Evidence for the Physical Interaction of NEU2 and GBA3.

Circular dichroism (CD) spectra at variable temperatures have been recorded for human cytosolic sialidase NEU2 in buffered water solutions and in the presence of divalent cations.
Title: Looking at human cytosolic sialidase NEU2 structural features with an interdisciplinary approach.

Q136K is a novel mutation in an expressed NEU2 protein that was discovered during crystallographic analysis.
Title: Preliminary crystallographic analysis of neuraminidase N2 from a new influenza A virus.

the present study demonstrated NEU2 expression to be detectable but very low in many human tissues and cells, and suggested possible functional roles in PC-3 prostate cancer cells.
Title: Human cytosolic sialidase NEU2-low general tissue expression but involvement in PC-3 prostate cancer cell survival.

The following NEU2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEU2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu16404	NM_005383.2 Latest version!	Homo sapiens neuraminidase 2 (NEU2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEU2 ( NM_005383.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005374.2 Homo sapiens neuraminidase 2 (NEU2), mRNA.

Price & Availability↑

CloneID	OHu16404
Clone ID Related Accession (Same CDS sequence)	NM_005383.2
Accession Version	NM_005383.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-29
Organism	Homo sapiens(human)
Product	sialidase-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC069151.1. On Jan 31, 2009 this sequence version replaced NM_005383.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC069151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000233840.3/ ENSP00000233840.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGCGTCCC TTCCTGTCCT GCAGAAGGAG AGCGTGTTCC AGTCGGGAGC CCATGCCTAC 
AGAATCCCTG CCCTGCTCTA CCTGCCTGGG CAGCAGTCCC TGCTGGCCTT CGCGGAACAG 
CGGGCAAGCA AGAAGGATGA GCACGCAGAG CTGATTGTCC TGCGCAGAGG AGACTACGAC 
GCACCCACCC ACCAGGTTCA GTGGCAAGCT CAGGAGGTGG TGGCCCAGGC CCGGCTGGAT 
GGCCACCGGT CCATGAACCC ATGCCCCTTG TATGACGCGC AGACGGGGAC CCTCTTCCTC 
TTCTTCATTG CCATCCCTGG GCAAGTCACG GAGCAACAGC AGCTGCAGAC CAGGGCCAAT 
GTGACGCGGC TGTGCCAAGT CACCAGCACT GACCACGGGA GGACCTGGAG CTCCCCCAGA 
GACCTCACTG ATGCGGCCAT CGGCCCAGCC TACCGGGAGT GGTCCACCTT TGCAGTGGGC 
CCGGGGCATT GTTTGCAGCT TCACGACAGG GCCCGGAGCC TGGTGGTGCC CGCCTACGCC 
TACCGGAAAC TTCACCCCAT CCAAAGGCCG ATCCCCTCTG CCTTCTGCTT CCTCAGCCAT 
GACCATGGGC GCACGTGGGC GCGAGGGCAC TTTGTGGCCC AGGACACCCT GGAGTGCCAG 
GTGGCCGAAG TCGAGACTGG GGAGCAGAGG GTGGTGACCC TCAACGCGAG AAGCCACCTC 
CGAGCCAGGG TCCAGGCCCA GAGCACCAAT GACGGGCTTG ATTTCCAGGA GTCTCAGCTG 
GTGAAGAAGC TGGTGGAGCC GCCGCCCCAG GGCTGCCAGG GGAGCGTCAT CAGCTTCCCC 
AGCCCCCGCT CGGGGCCTGG CTCCCCAGCC CAGTGGCTGC TCTACACTCA CCCCACACAC 
TCCTGGCAGA GGGCCGACCT GGGTGCCTAC CTCAACCCGC GACCTCCAGC CCCTGAGGCC 
TGGTCAGAGC CGGTACTGCT GGCCAAGGGC AGCTGTGCCT ACTCAGACCT CCAGAGCATG 
GGCACCGGCC CTGATGGGTC CCCCTTGTTT GGGTGTCTGT ACGAAGCCAA TGATTACGAG 
GAGATTGTCT TTCTCATGTT CACCCTGAAG CAAGCCTTCC CAGCTGAGTA CCTGCCTCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005374.2
CDS	1..1143
Translation	MASLPVLQKESVFQSGAHAYRIPALLYLPGQQSLLAFAEQRASKKDEHAELIVLRRGDYDAPTHQVQWQAQEVVAQARLDGHRSMNPCPLYDAQTGTLFLFFIAIPGQVTEQQQLQTRANVTRLCQVTSTDHGRTWSSPRDLTDAAIGPAYREWSTFAVGPGHCLQLHDRARSLVVPAYAYRKLHPIQRPIPSAFCFLSHDHGRTWARGHFVAQDTLECQVAEVETGEQRVVTLNARSHLRARVQAQSTNDGLDFQESQLVKKLVEPPPQGCQGSVISFPSPRSGPGSPAQWLLYTHPTHSWQRADLGAYLNPRPPAPEAWSEPVLLAKGSCAYSDLQSMGTGPDGSPLFGCLYEANDYEEIVFLMFTLKQAFPAEYLPQ

Target ORF information:

RefSeq Version	NM_005383.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neuraminidase 2 (NEU2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005383.2

ORF Insert Sequence:

ATGGCGTCCC TTCCTGTCCT GCAGAAGGAG AGCGTGTTCC AGTCGGGAGC CCATGCCTAC 
AGAATCCCTG CCCTGCTCTA CCTGCCTGGG CAGCAGTCCC TGCTGGCCTT CGCGGAACAG 
CGGGCAAGCA AGAAGGATGA GCACGCAGAG CTGATTGTCC TGCGCAGAGG AGACTACGAC 
GCACCCACCC ACCAGGTTCA GTGGCAAGCT CAGGAGGTGG TGGCCCAGGC CCGGCTGGAT 
GGCCACCGGT CCATGAACCC ATGCCCCTTG TATGACGCGC AGACGGGGAC CCTCTTCCTC 
TTCTTCATTG CCATCCCTGG GCAAGTCACG GAGCAACAGC AGCTGCAGAC CAGGGCCAAT 
GTGACGCGGC TGTGCCAAGT CACCAGCACT GACCACGGGA GGACCTGGAG CTCCCCCAGA 
GACCTCACTG ATGCGGCCAT CGGCCCAGCC TACCGGGAGT GGTCCACCTT TGCAGTGGGC 
CCGGGGCATT GTTTGCAGCT TCACGACAGG GCCCGGAGCC TGGTGGTGCC CGCCTACGCC 
TACCGGAAAC TTCACCCCAT CCAAAGGCCG ATCCCCTCTG CCTTCTGCTT CCTCAGCCAT 
GACCATGGGC GCACGTGGGC GCGAGGGCAC TTTGTGGCCC AGGACACCCT GGAGTGCCAG 
GTGGCCGAAG TCGAGACTGG GGAGCAGAGG GTGGTGACCC TCAACGCGAG AAGCCACCTC 
CGAGCCAGGG TCCAGGCCCA GAGCACCAAT GACGGGCTTG ATTTCCAGGA GTCTCAGCTG 
GTGAAGAAGC TGGTGGAGCC GCCGCCCCAG GGCTGCCAGG GGAGCGTCAT CAGCTTCCCC 
AGCCCCCGCT CGGGGCCTGG CTCCCCAGCC CAGTGGCTGC TCTACACTCA CCCCACACAC 
TCCTGGCAGA GGGCCGACCT GGGTGCCTAC CTCAACCCGC GACCTCCAGC CCCTGAGGCC 
TGGTCAGAGC CGGTACTGCT GGCCAAGGGC AGCTGTGCCT ACTCAGACCT CCAGAGCATG 
GGCACCGGCC CTGATGGGTC CCCCTTGTTT GGGTGTCTGT ACGAAGCCAA TGATTACGAG 
GAGATTGTCT TTCTCATGTT CACCCTGAAG CAAGCCTTCC CAGCTGAGTA CCTGCCTCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Membrane restructuring following in situ sialidase digestion of gangliosides: Complex model bilayers by synchrotron radiation reflectivity.
Biochimica et biophysica acta1859(5)845-851(2017 05)
Rondelli V,Brocca P,Fragneto G,Daillant J,Tringali C,Cantu' L,Del Favero E

Co-Expression of NEU2 and GBA3 Causes a Drastic Reduction in Cytosolic Sialyl Free N-glycans in Human MKN45 Stomach Cancer Cells-Evidence for the Physical Interaction of NEU2 and GBA3.
Biomolecules5(3)1499-514(2015 Jul)
Wang L,Seino J,Tomotake H,Funakoshi Y,Hirayama H,Suzuki T

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kov?cs IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barab?si AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

Looking at human cytosolic sialidase NEU2 structural features with an interdisciplinary approach.
Biochemistry53(32)5343-55(2014 Aug)
Monti E,Benaglia G,Mozzi A,Fusi P,Longhi G,Gangemi F,Castiglioni E,Woody RW,Fornili SL,Abbate S

Variants in the 1q21 risk region are associated with a visual endophenotype of autism and schizophrenia.
Genes, brain, and behavior13(2)144-51(2014 Feb)
Goodbourn PT,Bosten JM,Bargary G,Hogg RE,Lawrance-Owen AJ,Mollon JD

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NEU2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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