ORF » Species Summary » Homo sapiens » NEK2 cDNA ORF clone

NEK2 cDNA ORF clone, Homo sapiens(human)

The following NEK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu28072 NM_002497.4
Latest version!		 Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
OHu28010 NM_001204182.1
Latest version!		 Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu28076 NM_001204183.1
Latest version!		 Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu34410 XM_005273147.2
Latest version!		 Homo sapiens NIMA related kinase 2 (NEK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
View Old Accession Versions >>
OHu34410 XM_005273147.1 Homo sapiens NIMA related kinase 2 (NEK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu28072 NM_002497.3 Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    NEK2 ( NM_002497.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002488.1 Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu28072
    Clone ID Related Accession (Same CDS sequence) NM_002497.3 , NM_002497.4
    Accession Version NM_002497.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-17
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek2 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC407297.1, BT019729.1, AK223353.1 and BC043502.1. On Feb 17, 2011 this sequence version replaced NM_002497.2. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1, also known as NEK2A). The C-terminus (aa 399-445) of this isoform was shown to be responsible for its nucleolar localization (PMID:15161910). Isoforms 1 and 2 (which differ at the C-terminus) have also been reported to exhibit distinct pattern of expression during mitosis (PMID:11742531). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC043502.1, SRR1163658.443308.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCCTTCCC GGGCTGAGGA CTATGAAGTG TTGTACACCA TTGGCACAGG CTCCTACGGC 
    CGCTGCCAGA AGATCCGGAG GAAGAGTGAT GGCAAGATAT TAGTTTGGAA AGAACTTGAC 
    TATGGCTCCA TGACAGAAGC TGAGAAACAG ATGCTTGTTT CTGAAGTGAA TTTGCTTCGT 
    GAACTGAAAC ATCCAAACAT CGTTCGTTAC TATGATCGGA TTATTGACCG GACCAATACA 
    ACACTGTACA TTGTAATGGA ATATTGTGAA GGAGGGGATC TGGCTAGTGT AATTACAAAG 
    GGAACCAAGG AAAGGCAATA CTTAGATGAA GAGTTTGTTC TTCGAGTGAT GACTCAGTTG 
    ACTCTGGCCC TGAAGGAATG CCACAGACGA AGTGATGGTG GTCATACCGT ATTGCATCGG 
    GATCTGAAAC CAGCCAATGT TTTCCTGGAT GGCAAGCAAA ACGTCAAGCT TGGAGACTTT 
    GGGCTAGCTA GAATATTAAA CCATGACACG AGTTTTGCAA AAACATTTGT TGGCACACCT 
    TATTACATGT CTCCTGAACA AATGAATCGC ATGTCCTACA ATGAGAAATC AGATATCTGG 
    TCATTGGGCT GCTTGCTGTA TGAGTTATGT GCATTAATGC CTCCATTTAC AGCTTTTAGC 
    CAGAAAGAAC TCGCTGGGAA AATCAGAGAA GGCAAATTCA GGCGAATTCC ATACCGTTAC 
    TCTGATGAAT TGAATGAAAT TATTACGAGG ATGTTAAACT TAAAGGATTA CCATCGACCT 
    TCTGTTGAAG AAATTCTTGA GAACCCTTTA ATAGCAGATT TGGTTGCAGA CGAGCAAAGA 
    AGAAATCTTG AGAGAAGAGG GCGACAATTA GGAGAGCCAG AAAAATCGCA GGATTCCAGC 
    CCTGTATTGA GTGAGCTGAA ACTGAAGGAA ATTCAGTTAC AGGAGCGAGA GCGAGCTCTC 
    AAAGCAAGAG AAGAAAGATT GGAGCAGAAA GAACAGGAGC TTTGTGTTCG TGAGAGACTA 
    GCAGAGGACA AACTGGCTAG AGCAGAAAAT CTGTTGAAGA ACTACAGCTT GCTAAAGGAA 
    CGGAAGTTCC TGTCTCTGGC AAGTAATCCA GAACTTCTTA ATCTTCCATC CTCAGTAATT 
    AAGAAGAAAG TTCATTTCAG TGGGGAAAGT AAAGAGAACA TCATGAGGAG TGAGAATTCT 
    GAGAGTCAGC TCACATCTAA GTCCAAGTGC AAGGACCTGA AGAAAAGGCT TCACGCTGCC 
    CAGCTGCGGG CTCAAGCCCT GTCAGATATT GAGAAAAATT ACCAACTGAA AAGCAGACAG 
    ATCCTGGGCA TGCGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002488.1
    CDS152..1489
    Translation

    Target ORF information:

    RefSeq Version NM_002497.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_002497.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCCTTCCC GGGCTGAGGA CTATGAAGTG TTGTACACCA TTGGCACAGG CTCCTACGGC 
    CGCTGCCAGA AGATCCGGAG GAAGAGTGAT GGCAAGATAT TAGTTTGGAA AGAACTTGAC 
    TATGGCTCCA TGACAGAAGC TGAGAAACAG ATGCTTGTTT CTGAAGTGAA TTTGCTTCGT 
    GAACTGAAAC ATCCAAACAT CGTTCGTTAC TATGATCGGA TTATTGACCG GACCAATACA 
    ACACTGTACA TTGTAATGGA ATATTGTGAA GGAGGGGATC TGGCTAGTGT AATTACAAAG 
    GGAACCAAGG AAAGGCAATA CTTAGATGAA GAGTTTGTTC TTCGAGTGAT GACTCAGTTG 
    ACTCTGGCCC TGAAGGAATG CCACAGACGA AGTGATGGTG GTCATACCGT ATTGCATCGG 
    GATCTGAAAC CAGCCAATGT TTTCCTGGAT GGCAAGCAAA ACGTCAAGCT TGGAGACTTT 
    GGGCTAGCTA GAATATTAAA CCATGACACG AGTTTTGCAA AAACATTTGT TGGCACACCT 
    TATTACATGT CTCCTGAACA AATGAATCGC ATGTCCTACA ATGAGAAATC AGATATCTGG 
    TCATTGGGCT GCTTGCTGTA TGAGTTATGT GCATTAATGC CTCCATTTAC AGCTTTTAGC 
    CAGAAAGAAC TCGCTGGGAA AATCAGAGAA GGCAAATTCA GGCGAATTCC ATACCGTTAC 
    TCTGATGAAT TGAATGAAAT TATTACGAGG ATGTTAAACT TAAAGGATTA CCATCGACCT 
    TCTGTTGAAG AAATTCTTGA GAACCCTTTA ATAGCAGATT TGGTTGCAGA CGAGCAAAGA 
    AGAAATCTTG AGAGAAGAGG GCGACAATTA GGAGAGCCAG AAAAATCGCA GGATTCCAGC 
    CCTGTATTGA GTGAGCTGAA ACTGAAGGAA ATTCAGTTAC AGGAGCGAGA GCGAGCTCTC 
    AAAGCAAGAG AAGAAAGATT GGAGCAGAAA GAACAGGAGC TTTGTGTTCG TGAGAGACTA 
    GCAGAGGACA AACTGGCTAG AGCAGAAAAT CTGTTGAAGA ACTACAGCTT GCTAAAGGAA 
    CGGAAGTTCC TGTCTCTGGC AAGTAATCCA GAACTTCTTA ATCTTCCATC CTCAGTAATT 
    AAGAAGAAAG TTCATTTCAG TGGGGAAAGT AAAGAGAACA TCATGAGGAG TGAGAATTCT 
    GAGAGTCAGC TCACATCTAA GTCCAAGTGC AAGGACCTGA AGAAAAGGCT TCACGCTGCC 
    CAGCTGCGGG CTCAAGCCCT GTCAGATATT GAGAAAAATT ACCAACTGAA AAGCAGACAG 
    ATCCTGGGCA TGCGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK2 ( NM_002497.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002488.1 Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu28072
    Clone ID Related Accession (Same CDS sequence) NM_002497.3 , NM_002497.4
    Accession Version NM_002497.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek2 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC407297.1, BT019729.1, AK223353.1 and BC043502.1. On Nov 23, 2018 this sequence version replaced NM_002497.3. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1, also known as NEK2A). The C-terminus (aa 399-445) of this isoform was shown to be responsible for its nucleolar localization (PMID:15161910). Isoforms 1 and 2 (which differ at the C-terminus) have also been reported to exhibit distinct pattern of expression during mitosis (PMID:11742531). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC043502.1, SRR5189658.68294.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000366999.9/ ENSP00000355966.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCCTTCCC GGGCTGAGGA CTATGAAGTG TTGTACACCA TTGGCACAGG CTCCTACGGC 
    CGCTGCCAGA AGATCCGGAG GAAGAGTGAT GGCAAGATAT TAGTTTGGAA AGAACTTGAC 
    TATGGCTCCA TGACAGAAGC TGAGAAACAG ATGCTTGTTT CTGAAGTGAA TTTGCTTCGT 
    GAACTGAAAC ATCCAAACAT CGTTCGTTAC TATGATCGGA TTATTGACCG GACCAATACA 
    ACACTGTACA TTGTAATGGA ATATTGTGAA GGAGGGGATC TGGCTAGTGT AATTACAAAG 
    GGAACCAAGG AAAGGCAATA CTTAGATGAA GAGTTTGTTC TTCGAGTGAT GACTCAGTTG 
    ACTCTGGCCC TGAAGGAATG CCACAGACGA AGTGATGGTG GTCATACCGT ATTGCATCGG 
    GATCTGAAAC CAGCCAATGT TTTCCTGGAT GGCAAGCAAA ACGTCAAGCT TGGAGACTTT 
    GGGCTAGCTA GAATATTAAA CCATGACACG AGTTTTGCAA AAACATTTGT TGGCACACCT 
    TATTACATGT CTCCTGAACA AATGAATCGC ATGTCCTACA ATGAGAAATC AGATATCTGG 
    TCATTGGGCT GCTTGCTGTA TGAGTTATGT GCATTAATGC CTCCATTTAC AGCTTTTAGC 
    CAGAAAGAAC TCGCTGGGAA AATCAGAGAA GGCAAATTCA GGCGAATTCC ATACCGTTAC 
    TCTGATGAAT TGAATGAAAT TATTACGAGG ATGTTAAACT TAAAGGATTA CCATCGACCT 
    TCTGTTGAAG AAATTCTTGA GAACCCTTTA ATAGCAGATT TGGTTGCAGA CGAGCAAAGA 
    AGAAATCTTG AGAGAAGAGG GCGACAATTA GGAGAGCCAG AAAAATCGCA GGATTCCAGC 
    CCTGTATTGA GTGAGCTGAA ACTGAAGGAA ATTCAGTTAC AGGAGCGAGA GCGAGCTCTC 
    AAAGCAAGAG AAGAAAGATT GGAGCAGAAA GAACAGGAGC TTTGTGTTCG TGAGAGACTA 
    GCAGAGGACA AACTGGCTAG AGCAGAAAAT CTGTTGAAGA ACTACAGCTT GCTAAAGGAA 
    CGGAAGTTCC TGTCTCTGGC AAGTAATCCA GAACTTCTTA ATCTTCCATC CTCAGTAATT 
    AAGAAGAAAG TTCATTTCAG TGGGGAAAGT AAAGAGAACA TCATGAGGAG TGAGAATTCT 
    GAGAGTCAGC TCACATCTAA GTCCAAGTGC AAGGACCTGA AGAAAAGGCT TCACGCTGCC 
    CAGCTGCGGG CTCAAGCCCT GTCAGATATT GAGAAAAATT ACCAACTGAA AAGCAGACAG 
    ATCCTGGGCA TGCGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002488.1
    CDS143..1480
    Translation

    Target ORF information:

    RefSeq Version NM_002497.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_002497.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCCTTCCC GGGCTGAGGA CTATGAAGTG TTGTACACCA TTGGCACAGG CTCCTACGGC 
    CGCTGCCAGA AGATCCGGAG GAAGAGTGAT GGCAAGATAT TAGTTTGGAA AGAACTTGAC 
    TATGGCTCCA TGACAGAAGC TGAGAAACAG ATGCTTGTTT CTGAAGTGAA TTTGCTTCGT 
    GAACTGAAAC ATCCAAACAT CGTTCGTTAC TATGATCGGA TTATTGACCG GACCAATACA 
    ACACTGTACA TTGTAATGGA ATATTGTGAA GGAGGGGATC TGGCTAGTGT AATTACAAAG 
    GGAACCAAGG AAAGGCAATA CTTAGATGAA GAGTTTGTTC TTCGAGTGAT GACTCAGTTG 
    ACTCTGGCCC TGAAGGAATG CCACAGACGA AGTGATGGTG GTCATACCGT ATTGCATCGG 
    GATCTGAAAC CAGCCAATGT TTTCCTGGAT GGCAAGCAAA ACGTCAAGCT TGGAGACTTT 
    GGGCTAGCTA GAATATTAAA CCATGACACG AGTTTTGCAA AAACATTTGT TGGCACACCT 
    TATTACATGT CTCCTGAACA AATGAATCGC ATGTCCTACA ATGAGAAATC AGATATCTGG 
    TCATTGGGCT GCTTGCTGTA TGAGTTATGT GCATTAATGC CTCCATTTAC AGCTTTTAGC 
    CAGAAAGAAC TCGCTGGGAA AATCAGAGAA GGCAAATTCA GGCGAATTCC ATACCGTTAC 
    TCTGATGAAT TGAATGAAAT TATTACGAGG ATGTTAAACT TAAAGGATTA CCATCGACCT 
    TCTGTTGAAG AAATTCTTGA GAACCCTTTA ATAGCAGATT TGGTTGCAGA CGAGCAAAGA 
    AGAAATCTTG AGAGAAGAGG GCGACAATTA GGAGAGCCAG AAAAATCGCA GGATTCCAGC 
    CCTGTATTGA GTGAGCTGAA ACTGAAGGAA ATTCAGTTAC AGGAGCGAGA GCGAGCTCTC 
    AAAGCAAGAG AAGAAAGATT GGAGCAGAAA GAACAGGAGC TTTGTGTTCG TGAGAGACTA 
    GCAGAGGACA AACTGGCTAG AGCAGAAAAT CTGTTGAAGA ACTACAGCTT GCTAAAGGAA 
    CGGAAGTTCC TGTCTCTGGC AAGTAATCCA GAACTTCTTA ATCTTCCATC CTCAGTAATT 
    AAGAAGAAAG TTCATTTCAG TGGGGAAAGT AAAGAGAACA TCATGAGGAG TGAGAATTCT 
    GAGAGTCAGC TCACATCTAA GTCCAAGTGC AAGGACCTGA AGAAAAGGCT TCACGCTGCC 
    CAGCTGCGGG CTCAAGCCCT GTCAGATATT GAGAAAAATT ACCAACTGAA AAGCAGACAG 
    ATCCTGGGCA TGCGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK2 ( NM_001204182.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191111.1 Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu28010
    Clone ID Related Accession (Same CDS sequence) NM_001204182.1
    Accession Version NM_001204182.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek2 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC407297.1, BT019729.1 and AK225522.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) contains an alternate 3' terminal exon compared to variant 1. This results in a frame-shift and a shorter isoform (3) with a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225522.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCCTTCCC GGGCTGAGGA CTATGAAGTG TTGTACACCA TTGGCACAGG CTCCTACGGC 
    CGCTGCCAGA AGATCCGGAG GAAGAGTGAT GGCAAGATAT TAGTTTGGAA AGAACTTGAC 
    TATGGCTCCA TGACAGAAGC TGAGAAACAG ATGCTTGTTT CTGAAGTGAA TTTGCTTCGT 
    GAACTGAAAC ATCCAAACAT CGTTCGTTAC TATGATCGGA TTATTGACCG GACCAATACA 
    ACACTGTACA TTGTAATGGA ATATTGTGAA GGAGGGGATC TGGCTAGTGT AATTACAAAG 
    GGAACCAAGG AAAGGCAATA CTTAGATGAA GAGTTTGTTC TTCGAGTGAT GACTCAGTTG 
    ACTCTGGCCC TGAAGGAATG CCACAGACGA AGTGATGGTG GTCATACCGT ATTGCATCGG 
    GATCTGAAAC CAGCCAATGT TTTCCTGGAT GGCAAGCAAA ACGTCAAGCT TGGAGACTTT 
    GGGCTAGCTA GAATATTAAA CCATGACACG AGTTTTGCAA AAACATTTGT TGGCACACCT 
    TATTACATGT CTCCTGAACA AATGAATCGC ATGTCCTACA ATGAGAAATC AGATATCTGG 
    TCATTGGGCT GCTTGCTGTA TGAGTTATGT GCATTAATGC CTCCATTTAC AGCTTTTAGC 
    CAGAAAGAAC TCGCTGGGAA AATCAGAGAA GGCAAATTCA GGCGAATTCC ATACCGTTAC 
    TCTGATGAAT TGAATGAAAT TATTACGAGG ATGTTAAACT TAAAGGATTA CCATCGACCT 
    TCTGTTGAAG AAATTCTTGA GAACCCTTTA ATAGCAGATT TGGTTGCAGA CGAGCAAAGA 
    AGAAATCTTG AGAGAAGAGG GCGACAATTA GGAGAGCCAG AAAAATCGCA GGATTCCAGC 
    CCTGTATTGA GTGAGCTGAA ACTGAAGGAA ATTCAGTTAC AGGAGCGAGA GCGAGCTCTC 
    AAAGCAAGAG AAGAAAGATT GGAGCAGAAA GAACAGGAGC TTTGTGTTCG TGAGAGACTA 
    GCAGAGGACA AACTGGCTAG AGCAGAAAAT CTGTTGAAGA ACTACAGCTT GCTAAAGGAA 
    CGGAAGTTCC TGTCTCTGGC AAGTAATCCA GAGTCTCACT TTGTTGCCCA GGCTGGAATG 
    CAGTGGTGTG ATCACAGCTC AATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191111.1
    CDS152..1318
    Translation

    Target ORF information:

    RefSeq Version NM_001204182.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001204182.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCCTTCCC GGGCTGAGGA CTATGAAGTG TTGTACACCA TTGGCACAGG CTCCTACGGC 
    CGCTGCCAGA AGATCCGGAG GAAGAGTGAT GGCAAGATAT TAGTTTGGAA AGAACTTGAC 
    TATGGCTCCA TGACAGAAGC TGAGAAACAG ATGCTTGTTT CTGAAGTGAA TTTGCTTCGT 
    GAACTGAAAC ATCCAAACAT CGTTCGTTAC TATGATCGGA TTATTGACCG GACCAATACA 
    ACACTGTACA TTGTAATGGA ATATTGTGAA GGAGGGGATC TGGCTAGTGT AATTACAAAG 
    GGAACCAAGG AAAGGCAATA CTTAGATGAA GAGTTTGTTC TTCGAGTGAT GACTCAGTTG 
    ACTCTGGCCC TGAAGGAATG CCACAGACGA AGTGATGGTG GTCATACCGT ATTGCATCGG 
    GATCTGAAAC CAGCCAATGT TTTCCTGGAT GGCAAGCAAA ACGTCAAGCT TGGAGACTTT 
    GGGCTAGCTA GAATATTAAA CCATGACACG AGTTTTGCAA AAACATTTGT TGGCACACCT 
    TATTACATGT CTCCTGAACA AATGAATCGC ATGTCCTACA ATGAGAAATC AGATATCTGG 
    TCATTGGGCT GCTTGCTGTA TGAGTTATGT GCATTAATGC CTCCATTTAC AGCTTTTAGC 
    CAGAAAGAAC TCGCTGGGAA AATCAGAGAA GGCAAATTCA GGCGAATTCC ATACCGTTAC 
    TCTGATGAAT TGAATGAAAT TATTACGAGG ATGTTAAACT TAAAGGATTA CCATCGACCT 
    TCTGTTGAAG AAATTCTTGA GAACCCTTTA ATAGCAGATT TGGTTGCAGA CGAGCAAAGA 
    AGAAATCTTG AGAGAAGAGG GCGACAATTA GGAGAGCCAG AAAAATCGCA GGATTCCAGC 
    CCTGTATTGA GTGAGCTGAA ACTGAAGGAA ATTCAGTTAC AGGAGCGAGA GCGAGCTCTC 
    AAAGCAAGAG AAGAAAGATT GGAGCAGAAA GAACAGGAGC TTTGTGTTCG TGAGAGACTA 
    GCAGAGGACA AACTGGCTAG AGCAGAAAAT CTGTTGAAGA ACTACAGCTT GCTAAAGGAA 
    CGGAAGTTCC TGTCTCTGGC AAGTAATCCA GAGTCTCACT TTGTTGCCCA GGCTGGAATG 
    CAGTGGTGTG ATCACAGCTC AATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK2 ( NM_001204183.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191112.1 Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu28076
    Clone ID Related Accession (Same CDS sequence) NM_001204183.1
    Accession Version NM_001204183.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek2 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC407297.1, BT019729.1, AY045701.1 and BU517064.1. Transcript Variant: This variant (2) differs at the 3' end compared to variant 1. This results in a frame-shift and a shorter isoform (2, also known as NEK2B) with a distinct C-terminus compared to isoform 1. Isoforms 1 and 2 have been reported to exhibit distinct pattern of expression during mitosis (PMID:11742531). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY045701.1, SRR1163657.570346.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCCTTCCC GGGCTGAGGA CTATGAAGTG TTGTACACCA TTGGCACAGG CTCCTACGGC 
    CGCTGCCAGA AGATCCGGAG GAAGAGTGAT GGCAAGATAT TAGTTTGGAA AGAACTTGAC 
    TATGGCTCCA TGACAGAAGC TGAGAAACAG ATGCTTGTTT CTGAAGTGAA TTTGCTTCGT 
    GAACTGAAAC ATCCAAACAT CGTTCGTTAC TATGATCGGA TTATTGACCG GACCAATACA 
    ACACTGTACA TTGTAATGGA ATATTGTGAA GGAGGGGATC TGGCTAGTGT AATTACAAAG 
    GGAACCAAGG AAAGGCAATA CTTAGATGAA GAGTTTGTTC TTCGAGTGAT GACTCAGTTG 
    ACTCTGGCCC TGAAGGAATG CCACAGACGA AGTGATGGTG GTCATACCGT ATTGCATCGG 
    GATCTGAAAC CAGCCAATGT TTTCCTGGAT GGCAAGCAAA ACGTCAAGCT TGGAGACTTT 
    GGGCTAGCTA GAATATTAAA CCATGACACG AGTTTTGCAA AAACATTTGT TGGCACACCT 
    TATTACATGT CTCCTGAACA AATGAATCGC ATGTCCTACA ATGAGAAATC AGATATCTGG 
    TCATTGGGCT GCTTGCTGTA TGAGTTATGT GCATTAATGC CTCCATTTAC AGCTTTTAGC 
    CAGAAAGAAC TCGCTGGGAA AATCAGAGAA GGCAAATTCA GGCGAATTCC ATACCGTTAC 
    TCTGATGAAT TGAATGAAAT TATTACGAGG ATGTTAAACT TAAAGGATTA CCATCGACCT 
    TCTGTTGAAG AAATTCTTGA GAACCCTTTA ATAGCAGATT TGGTTGCAGA CGAGCAAAGA 
    AGAAATCTTG AGAGAAGAGG GCGACAATTA GGAGAGCCAG AAAAATCGCA GGATTCCAGC 
    CCTGTATTGA GTGAGCTGAA ACTGAAGGAA ATTCAGTTAC AGGAGCGAGA GCGAGCTCTC 
    AAAGCAAGAG AAGAAAGATT GGAGCAGAAA GAACAGGAGC TTTGTGTTCG TGAGAGACTA 
    GCAGAGGACA AACTGGCTAG AGCAGAAAAT CTGTTGAAGA ACTACAGCTT GCTAAAGGAA 
    CGGAAGTTCC TGTCTCTGGC AAGTAATCCA GGTATGAGAA TCAACTTGGT CAACAGAAGC 
    TGGTGCTACA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191112.1
    CDS152..1306
    Translation

    Target ORF information:

    RefSeq Version NM_001204183.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 2 (NEK2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001204183.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCCTTCCC GGGCTGAGGA CTATGAAGTG TTGTACACCA TTGGCACAGG CTCCTACGGC 
    CGCTGCCAGA AGATCCGGAG GAAGAGTGAT GGCAAGATAT TAGTTTGGAA AGAACTTGAC 
    TATGGCTCCA TGACAGAAGC TGAGAAACAG ATGCTTGTTT CTGAAGTGAA TTTGCTTCGT 
    GAACTGAAAC ATCCAAACAT CGTTCGTTAC TATGATCGGA TTATTGACCG GACCAATACA 
    ACACTGTACA TTGTAATGGA ATATTGTGAA GGAGGGGATC TGGCTAGTGT AATTACAAAG 
    GGAACCAAGG AAAGGCAATA CTTAGATGAA GAGTTTGTTC TTCGAGTGAT GACTCAGTTG 
    ACTCTGGCCC TGAAGGAATG CCACAGACGA AGTGATGGTG GTCATACCGT ATTGCATCGG 
    GATCTGAAAC CAGCCAATGT TTTCCTGGAT GGCAAGCAAA ACGTCAAGCT TGGAGACTTT 
    GGGCTAGCTA GAATATTAAA CCATGACACG AGTTTTGCAA AAACATTTGT TGGCACACCT 
    TATTACATGT CTCCTGAACA AATGAATCGC ATGTCCTACA ATGAGAAATC AGATATCTGG 
    TCATTGGGCT GCTTGCTGTA TGAGTTATGT GCATTAATGC CTCCATTTAC AGCTTTTAGC 
    CAGAAAGAAC TCGCTGGGAA AATCAGAGAA GGCAAATTCA GGCGAATTCC ATACCGTTAC 
    TCTGATGAAT TGAATGAAAT TATTACGAGG ATGTTAAACT TAAAGGATTA CCATCGACCT 
    TCTGTTGAAG AAATTCTTGA GAACCCTTTA ATAGCAGATT TGGTTGCAGA CGAGCAAAGA 
    AGAAATCTTG AGAGAAGAGG GCGACAATTA GGAGAGCCAG AAAAATCGCA GGATTCCAGC 
    CCTGTATTGA GTGAGCTGAA ACTGAAGGAA ATTCAGTTAC AGGAGCGAGA GCGAGCTCTC 
    AAAGCAAGAG AAGAAAGATT GGAGCAGAAA GAACAGGAGC TTTGTGTTCG TGAGAGACTA 
    GCAGAGGACA AACTGGCTAG AGCAGAAAAT CTGTTGAAGA ACTACAGCTT GCTAAAGGAA 
    CGGAAGTTCC TGTCTCTGGC AAGTAATCCA GGTATGAGAA TCAACTTGGT CAACAGAAGC 
    TGGTGCTACA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK2 ( XM_005273147.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273204.1 Homo sapiens NIMA related kinase 2 (NEK2), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu34410
    Clone ID Related Accession (Same CDS sequence) XM_005273147.2 , XM_005273147.1
    Accession Version XM_005273147.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005273147.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCTTCCC GGGCTGAGGA CTATGAAGTG TTGTACACCA TTGGCACAGG CTCCTACGGC 
    CGCTGCCAGA AGATCCGGAG GAAGAGTGAT GGCAAGATAT TAGTTTGGAA AGAACTTGAC 
    TATGGCTCCA TGACAGAAGC TGAGAAACAG ATGCTTGTTT CTGAAGTGAA TTTGCTTCGT 
    GAACTGAAAC ATCCAAACAT CGTTCGTTAC TATGATCGGA TTATTGACCG GACCAATACA 
    ACACTGTACA TTGTAATGGA ATATTGTGAA GGAGGGGATC TGGCTAGTGT AATTACAAAG 
    GGAACCAAGG AAAGGCAATA CTTAGATGAA GAGTTTGTTC TTCGAGTGAT GACTCAGTTG 
    ACTCTGGCCC TGAAGGAATG CCACAGACGA AGTGATGGTG GTCATACCGT ATTGCATCGG 
    GATCTGAAAC CAGCCAATGT TTTCCTGGAT GGCAAGCAAA ACGTCAAGCT TGGAGACTTT 
    GGGCTAGCTA GAATATTAAA CCATGACACG AGTTTTGCAA AAACATTTGT TGGCACACCT 
    TATTACATGT CTCCTGAACA AATGAATCGC ATGTCCTACA ATGAGAAATC AGATATCTGG 
    TCATTGGGCT GCTTGCTGTA TGAGTTATGT GCATTAATGC CTCCATTTAC AGCTTTTAGC 
    CAGAAAGAAC TCGCTGGGAA AATCAGAGAA GGCAAATTCA GGCGAATTCC ATACCGTTAC 
    TCTGATGAAT TGAATGAAAT TATTACGAGG ATGTTAAACT TAAAGGATTA CCATCGACCT 
    TCTGTTGAAG AAATTCTTGA GAACCCTTTA ATAGCAGATT TGGTTGCAGA CGAGCAAAGA 
    AGAAATCTTG AGAGAAGAGG GCGACAATTA GGAGAGCCAG AAAAATCGCA GGATTCCAGC 
    CCTGTATTGA GTGAGCTGAA ACTGAAGGAA ATTCAGTTAC AGGAGCGAGA GCGAGCTCTC 
    AAAGCAAGAG AAGAAAGATT GGAGCAGAAA GAACAGGAGC TTTGTGTTCG TGAGAGACTA 
    GCAGAGGACA AACTGGCTAG AGCAGAAAAT CTGTTGAAGA ACTACAGCTT GCTAAAGGAA 
    CGGAAGTTCC TGTCTCTGGC AAGTAATCCA GTAATTAAGA AGAAAGTTCA TTTCAGTGGG 
    GAAAGTAAAG AGAACATCAT GAGGAGTGAG AATTCTGAGA GTCAGCTCAC ATCTAAGTCC 
    AAGTGCAAGG ACCTGAAGAA AAGGCTTCAC GCTGCCCAGC TGCGGGCTCA AGCCCTGTCA 
    GATATTGAGA AAAATTACCA ACTGAAAAGC AGACAGATCC TGGGCATGCG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005273204.1
    CDS147..1460
    Translation

    Target ORF information:

    RefSeq Version XM_005273147.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 2 (NEK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005273147.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCTTCCC GGGCTGAGGA CTATGAAGTG TTGTACACCA TTGGCACAGG CTCCTACGGC 
    CGCTGCCAGA AGATCCGGAG GAAGAGTGAT GGCAAGATAT TAGTTTGGAA AGAACTTGAC 
    TATGGCTCCA TGACAGAAGC TGAGAAACAG ATGCTTGTTT CTGAAGTGAA TTTGCTTCGT 
    GAACTGAAAC ATCCAAACAT CGTTCGTTAC TATGATCGGA TTATTGACCG GACCAATACA 
    ACACTGTACA TTGTAATGGA ATATTGTGAA GGAGGGGATC TGGCTAGTGT AATTACAAAG 
    GGAACCAAGG AAAGGCAATA CTTAGATGAA GAGTTTGTTC TTCGAGTGAT GACTCAGTTG 
    ACTCTGGCCC TGAAGGAATG CCACAGACGA AGTGATGGTG GTCATACCGT ATTGCATCGG 
    GATCTGAAAC CAGCCAATGT TTTCCTGGAT GGCAAGCAAA ACGTCAAGCT TGGAGACTTT 
    GGGCTAGCTA GAATATTAAA CCATGACACG AGTTTTGCAA AAACATTTGT TGGCACACCT 
    TATTACATGT CTCCTGAACA AATGAATCGC ATGTCCTACA ATGAGAAATC AGATATCTGG 
    TCATTGGGCT GCTTGCTGTA TGAGTTATGT GCATTAATGC CTCCATTTAC AGCTTTTAGC 
    CAGAAAGAAC TCGCTGGGAA AATCAGAGAA GGCAAATTCA GGCGAATTCC ATACCGTTAC 
    TCTGATGAAT TGAATGAAAT TATTACGAGG ATGTTAAACT TAAAGGATTA CCATCGACCT 
    TCTGTTGAAG AAATTCTTGA GAACCCTTTA ATAGCAGATT TGGTTGCAGA CGAGCAAAGA 
    AGAAATCTTG AGAGAAGAGG GCGACAATTA GGAGAGCCAG AAAAATCGCA GGATTCCAGC 
    CCTGTATTGA GTGAGCTGAA ACTGAAGGAA ATTCAGTTAC AGGAGCGAGA GCGAGCTCTC 
    AAAGCAAGAG AAGAAAGATT GGAGCAGAAA GAACAGGAGC TTTGTGTTCG TGAGAGACTA 
    GCAGAGGACA AACTGGCTAG AGCAGAAAAT CTGTTGAAGA ACTACAGCTT GCTAAAGGAA 
    CGGAAGTTCC TGTCTCTGGC AAGTAATCCA GTAATTAAGA AGAAAGTTCA TTTCAGTGGG 
    GAAAGTAAAG AGAACATCAT GAGGAGTGAG AATTCTGAGA GTCAGCTCAC ATCTAAGTCC 
    AAGTGCAAGG ACCTGAAGAA AAGGCTTCAC GCTGCCCAGC TGCGGGCTCA AGCCCTGTCA 
    GATATTGAGA AAAATTACCA ACTGAAAAGC AGACAGATCC TGGGCATGCG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEK2 ( XM_005273147.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273204.1 Homo sapiens NIMA related kinase 2 (NEK2), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu34410
    Clone ID Related Accession (Same CDS sequence) XM_005273147.2 , XM_005273147.1
    Accession Version XM_005273147.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCTTCCC GGGCTGAGGA CTATGAAGTG TTGTACACCA TTGGCACAGG CTCCTACGGC 
    CGCTGCCAGA AGATCCGGAG GAAGAGTGAT GGCAAGATAT TAGTTTGGAA AGAACTTGAC 
    TATGGCTCCA TGACAGAAGC TGAGAAACAG ATGCTTGTTT CTGAAGTGAA TTTGCTTCGT 
    GAACTGAAAC ATCCAAACAT CGTTCGTTAC TATGATCGGA TTATTGACCG GACCAATACA 
    ACACTGTACA TTGTAATGGA ATATTGTGAA GGAGGGGATC TGGCTAGTGT AATTACAAAG 
    GGAACCAAGG AAAGGCAATA CTTAGATGAA GAGTTTGTTC TTCGAGTGAT GACTCAGTTG 
    ACTCTGGCCC TGAAGGAATG CCACAGACGA AGTGATGGTG GTCATACCGT ATTGCATCGG 
    GATCTGAAAC CAGCCAATGT TTTCCTGGAT GGCAAGCAAA ACGTCAAGCT TGGAGACTTT 
    GGGCTAGCTA GAATATTAAA CCATGACACG AGTTTTGCAA AAACATTTGT TGGCACACCT 
    TATTACATGT CTCCTGAACA AATGAATCGC ATGTCCTACA ATGAGAAATC AGATATCTGG 
    TCATTGGGCT GCTTGCTGTA TGAGTTATGT GCATTAATGC CTCCATTTAC AGCTTTTAGC 
    CAGAAAGAAC TCGCTGGGAA AATCAGAGAA GGCAAATTCA GGCGAATTCC ATACCGTTAC 
    TCTGATGAAT TGAATGAAAT TATTACGAGG ATGTTAAACT TAAAGGATTA CCATCGACCT 
    TCTGTTGAAG AAATTCTTGA GAACCCTTTA ATAGCAGATT TGGTTGCAGA CGAGCAAAGA 
    AGAAATCTTG AGAGAAGAGG GCGACAATTA GGAGAGCCAG AAAAATCGCA GGATTCCAGC 
    CCTGTATTGA GTGAGCTGAA ACTGAAGGAA ATTCAGTTAC AGGAGCGAGA GCGAGCTCTC 
    AAAGCAAGAG AAGAAAGATT GGAGCAGAAA GAACAGGAGC TTTGTGTTCG TGAGAGACTA 
    GCAGAGGACA AACTGGCTAG AGCAGAAAAT CTGTTGAAGA ACTACAGCTT GCTAAAGGAA 
    CGGAAGTTCC TGTCTCTGGC AAGTAATCCA GTAATTAAGA AGAAAGTTCA TTTCAGTGGG 
    GAAAGTAAAG AGAACATCAT GAGGAGTGAG AATTCTGAGA GTCAGCTCAC ATCTAAGTCC 
    AAGTGCAAGG ACCTGAAGAA AAGGCTTCAC GCTGCCCAGC TGCGGGCTCA AGCCCTGTCA 
    GATATTGAGA AAAATTACCA ACTGAAAAGC AGACAGATCC TGGGCATGCG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005273204.1
    CDS149..1462
    Translation

    Target ORF information:

    RefSeq Version XM_005273147.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 2 (NEK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005273147.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCTTCCC GGGCTGAGGA CTATGAAGTG TTGTACACCA TTGGCACAGG CTCCTACGGC 
    CGCTGCCAGA AGATCCGGAG GAAGAGTGAT GGCAAGATAT TAGTTTGGAA AGAACTTGAC 
    TATGGCTCCA TGACAGAAGC TGAGAAACAG ATGCTTGTTT CTGAAGTGAA TTTGCTTCGT 
    GAACTGAAAC ATCCAAACAT CGTTCGTTAC TATGATCGGA TTATTGACCG GACCAATACA 
    ACACTGTACA TTGTAATGGA ATATTGTGAA GGAGGGGATC TGGCTAGTGT AATTACAAAG 
    GGAACCAAGG AAAGGCAATA CTTAGATGAA GAGTTTGTTC TTCGAGTGAT GACTCAGTTG 
    ACTCTGGCCC TGAAGGAATG CCACAGACGA AGTGATGGTG GTCATACCGT ATTGCATCGG 
    GATCTGAAAC CAGCCAATGT TTTCCTGGAT GGCAAGCAAA ACGTCAAGCT TGGAGACTTT 
    GGGCTAGCTA GAATATTAAA CCATGACACG AGTTTTGCAA AAACATTTGT TGGCACACCT 
    TATTACATGT CTCCTGAACA AATGAATCGC ATGTCCTACA ATGAGAAATC AGATATCTGG 
    TCATTGGGCT GCTTGCTGTA TGAGTTATGT GCATTAATGC CTCCATTTAC AGCTTTTAGC 
    CAGAAAGAAC TCGCTGGGAA AATCAGAGAA GGCAAATTCA GGCGAATTCC ATACCGTTAC 
    TCTGATGAAT TGAATGAAAT TATTACGAGG ATGTTAAACT TAAAGGATTA CCATCGACCT 
    TCTGTTGAAG AAATTCTTGA GAACCCTTTA ATAGCAGATT TGGTTGCAGA CGAGCAAAGA 
    AGAAATCTTG AGAGAAGAGG GCGACAATTA GGAGAGCCAG AAAAATCGCA GGATTCCAGC 
    CCTGTATTGA GTGAGCTGAA ACTGAAGGAA ATTCAGTTAC AGGAGCGAGA GCGAGCTCTC 
    AAAGCAAGAG AAGAAAGATT GGAGCAGAAA GAACAGGAGC TTTGTGTTCG TGAGAGACTA 
    GCAGAGGACA AACTGGCTAG AGCAGAAAAT CTGTTGAAGA ACTACAGCTT GCTAAAGGAA 
    CGGAAGTTCC TGTCTCTGGC AAGTAATCCA GTAATTAAGA AGAAAGTTCA TTTCAGTGGG 
    GAAAGTAAAG AGAACATCAT GAGGAGTGAG AATTCTGAGA GTCAGCTCAC ATCTAAGTCC 
    AAGTGCAAGG ACCTGAAGAA AAGGCTTCAC GCTGCCCAGC TGCGGGCTCA AGCCCTGTCA 
    GATATTGAGA AAAATTACCA ACTGAAAAGC AGACAGATCC TGGGCATGCG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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