NEDD4 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011521625.2	XP_011519927.1	E3 ubiquitin-protein ligase NEDD4 isoform X2
NM_198400.3	NP_940682.2	E3 ubiquitin-protein ligase NEDD4 isoform 2
XM_011521624.3	XP_011519926.1	E3 ubiquitin-protein ligase NEDD4 isoform X1
XM_011521624.2	XP_011519926.1	E3 ubiquitin-protein ligase NEDD4 isoform X1
NM_006154.3	NP_006145.2	E3 ubiquitin-protein ligase NEDD4 isoform 1
XM_011521626.1	XP_011519928.1	E3 ubiquitin-protein ligase NEDD4 isoform X2
NM_001284339.1	NP_001271268.1	E3 ubiquitin-protein ligase NEDD4 isoform 4
NM_001284340.1	NP_001271269.1	E3 ubiquitin-protein ligase NEDD4 isoform 5
XM_011521627.1	XP_011519929.1	E3 ubiquitin-protein ligase NEDD4 isoform X3
NM_001329212.2	NP_001316141.1	E3 ubiquitin-protein ligase NEDD4 isoform 6
NM_006154.4	NP_006145.2	E3 ubiquitin-protein ligase NEDD4 isoform 1
NM_001284338.1	NP_001271267.1	E3 ubiquitin-protein ligase NEDD4 isoform 3
NM_001329212.1	NP_001316141.1	E3 ubiquitin-protein ligase NEDD4 isoform 6
XM_011521625.3	XP_011519927.1	E3 ubiquitin-protein ligase NEDD4 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20953	NM_001284338.1 Latest version!	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu100779	NM_001329212.2 Latest version!	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OHu21031	NM_006154.4 Latest version!	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu59237	XM_011521624.3 Latest version!	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu59238	XM_011521625.3 Latest version!	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu59238	XM_011521626.1 Latest version!	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu59239	XM_011521627.1 Latest version!	Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu20676	NM_001284340.1 Latest version!	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu21480	NM_198400.3 Latest version!	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu21763	NM_001284339.1 Latest version!	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu59237	XM_011521624.2	Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu21031	NM_006154.3	Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu100779	NM_001329212.1	Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OHu59238	XM_011521625.2	Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

NEDD4 ( NM_001284338.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271267.1 Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu20953
Clone ID Related Accession (Same CDS sequence)	NM_001284338.1
Accession Version	NM_001284338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832063.1, BC136605.1, BC144285.1 and AC009997.7. On Sep 28, 2013 this sequence version replaced XM_005254406.1. Transcript Variant: This variant (3) contains three alternate in-frame exons, compared to variant 2. The encoded isoform (3) is longer than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC136605.1, AL833300.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA CCCTTTGTCA 
GAAACCTCCG GAGATGACTT GGATAGCCAT GTTCACATGT GCTTCAAAAG ACCAACACGG 
ATTTCAACGT CTAACGTTGT TCAAATGAAG CTGACTCCCA GACAGACTGC ACTAGCTCCG 
TTAATAAAGG AAAACGTTCA GTCTCAAGAA AGATCATCTG TTCCCTCATC TGAAAATGTT 
AATAAAAAGA GCAGCTGTCT ACAGATTTCA CTACAGCCAA CAAGGTACAG TGGATATCTT 
CAGTCTAGCA ATGTCTTAGC TGATAGTGAT GATGCTTCGT TTACTTGTAT CTTGAAGGAT 
GGTATTTACA GTAGTGCTGT GGTCGATAAT GAATTGAATG CTGTGAATGA TGGTCACCTT 
GTAAGCAGTC CAGCCATTTG TAGTGGTAGC CTTAGTAACT TTTCAACCAG TGATAATGGG 
TCTTACAGCA GCAACGGTAG TGATTTTGGG TCATGTGCAA GTATCACAAG TGGAGGTTCA 
TATACTAACA GTGTCATCAG TGACAGTAGT AGTTATACTT TTCCACCAAG TGATGATACT 
TTTTTGGGTG GAAACTTACC TTCTGACAGC ACCTCCAATA GAAGTGTGCC AAACAGGAAT 
ACTACTCCTT GTGAAATTTT TTCAAGAAGT ACAAGTACAG ATCCTTTTGT CCAGGATGAC 
TTGGAACATG GATTAGAGAT TATGAAATTG CCAGTGAGCA GGAACACAAA AATTCCACTA 
AAACGTTACT CCTCCTTAGT CATTTTTCCT AGGAGTCCTT CAACTACCCG ACCGACTTCT 
CCAACAAGTC TGTGTACTCT TCTGAGCAAA GGATCCTATC AAACTTCACA CCAGTTTATT 
ATTTCTCCTA GTGAAATTGC ACATAATGAG GATGGCACTA GTGCTAAAGG ATTTCTTTCA 
ACAGCTGTCA ATGGACTTCG GTTATCTAAA ACAATTTGTA CCCCCGGAGA AGTAAGAGAC 
ATACGGCCGC TTCACAGGAA GGGCTCGTTA CAGAAGAAAA TTGTTCTTTC GAATAATACT 
CCCAGACAGA CTGTCTGTGA AAAGTCATCT GAAGGATATT CTTGTGTTTC AGTGCATTTC 
ACCCAACGAA AAGCAGCTAC ATTAGACTGT GAAACAACAA ATGGTGATTG TAAACCAGAA 
ATGTCAGAAA TTAAGCTTAA TTCTGATTCA GAGTATATTA AGCTCATGCA TAGGACATCT 
GCATGTTTGC CATCCTCCCA AAATGTAGAT TGTCAAATAA ATATCAATGG AGAATTGGAA 
AGACCACATT CACAGATGAA CAAAAACCAT GGTATTTTAC GAAGAAGTAT TTCATTGGGA 
GGAGCTTATC CAAATATTTC CTGTCTATCC AGCCTTAAGC ACAATTGTTC TAAAGGGGGA 
CCATCTCAGT TACTCATAAA GTTTGCATCT GGAAATGAAG GTAAAGTGGA TAATTTATCA 
AGAGACAGCA ACAGAGATTG CACAAATGAA CTGTCTAATT CTTGCAAGAC AAGAGATGAT 
TTCCTAGGTC AAGTGGATGT TCCACTTTAT CCATTACCGA CAGAAAATCC AAGATTGGAG 
AGACCATATA CATTTAAGGA TTTTGTTCTT CATCCAAGAA GTCACAAATC AAGAGTTAAA 
GGTTATCTGA GACTAAAAAT GACTTATTTA CCTAAAACCA GTGGCTCAGA AGATGATAAT 
GCAGAACAGG CTGAGGAATT AGAGCCTGGC TGGGTTGTTT TGGACCAACC AGATGCTGCT 
TGCCATTTGC AGCAACAACA AGAACCTTCT CCTCTACCTC CAGGGTGGGA AGAGAGGCAG 
GATATCCTTG GAAGGACCTA TTATGTAAAC CATGAATCTA GAAGAACACA GTGGAAAAGA 
CCAACCCCTC AGGACAACCT AACAGATGCT GAGAATGGCA ACATTCAACT GCAAGCACAA 
CGTGCATTTA CCACCAGGCG GCAGATATCC GAGGAAACAG AAAGTGTTGA CAACCGAGAG 
TCTTCCGAGA ACTGGGAAAT TATAAGAGAA GATGAAGCCA CCATGTATAG CAACCAGGCC 
TTCCCATCAC CTCCACCGTC AAGTAACTTG GATGTTCCAA CTCATCTTGC AGAAGAATTG 
AATGCCAGAC TCACCATTTT TGGAAATTCA GCCGTGAGCC AGCCAGCATC GAGCTCAAAT 
CATTCCAGCA GAAGAGGCAG CTTACAAGCC TATACTTTTG AGGAACAACC TACACTTCCT 
GTGCTTTTGC CTACTTCATC TGGATTACCA CCAGGTTGGG AAGAAAAACA AGATGAAAGA 
GGAAGATCAT ATTATGTAGA TCACAATTCC AGAACGACTA CTTGGACAAA GCCCACTGTA 
CAGGCCACAG TGGAGACCAG TCAGCTGACC TCAAGCCAGA GTTCTGCAGG CCCTCAATCA 
CAAGCCTCCA CCAGTGATTC AGGCCAGCAG GTGACCCAGC CATCTGAAAT TGAGCAAGGA 
TTCCTTCCTA AAGGCTGGGA AGTCCGGCAT GCACCAAATG GGAGGCCTTT CTTTATTGAC 
CACAACACTA AAACCACCAC CTGGGAAGAT CCAAGATTGA AAATTCCAGC CCATCTGAGA 
GGAAAGACAT CACTTGATAC TTCCAATGAT CTAGGGCCTT TACCTCCAGG ATGGGAAGAG 
AGAACTCACA CAGATGGAAG AATCTTCTAC ATAAATCACA ATATAAAAAG AACACAATGG 
GAAGATCCTC GGTTGGAGAA TGTAGCAATA ACTGGACCAG CAGTGCCCTA CTCCAGGGAT 
TACAAAAGAA AGTATGAGTT CTTCCGAAGA AAGTTGAAGA AGCAGAATGA CATTCCAAAC 
AAATTTGAAA TGAAACTTCG CCGAGCAACT GTTCTTGAAG ACTCTTACCG GAGAATTATG 
GGTGTCAAGA GAGCAGACTT CCTGAAGGCT CGACTGTGGA TTGAGTTTGA TGGTGAAAAG 
GGATTGGATT ATGGAGGAGT TGCCAGAGAA TGGTTCTTCC TGATCTCAAA GGAAATGTTT 
AACCCTTATT ATGGGTTGTT TGAATATTCT GCTACGGACA ATTATACCCT ACAGATAAAT 
CCAAACTCTG GATTGTGTAA CGAAGATCAC CTCTCTTACT TCAAGTTTAT TGGTCGGGTA 
GCTGGAATGG CAGTTTATCA TGGCAAACTG TTGGATGGTT TTTTCATCCG CCCATTTTAC 
AAGATGATGC TTCACAAACC AATAACCCTT CATGATATGG AATCTGTGGA TAGTGAATAT 
TACAATTCCC TAAGATGGAT TCTTGAAAAT GACCCAACAG AATTGGACCT CAGGTTTATC 
ATAGATGAAG AACTTTTTGG ACAGACACAT CAACATGAGC TGAAAAATGG TGGATCAGAA 
ATAGTTGTCA CCAATAAGAA CAAAAAGGAA TATATTTATC TTGTAATACA ATGGCGATTT 
GTAAACCGAA TCCAGAAGCA AATGGCTGCT TTTAAAGAGG GATTCTTTGA ACTAATACCA 
CAGGATCTCA TCAAAATTTT TGATGAAAAT GAACTAGAGC TTCTTATGTG TGGACTGGGA 
GATGTTGATG TGAATGACTG GAGGGAACAT ACAAAGTATA AAAATGGCTA CAGTGCAAAT 
CATCAGGTTA TACAGTGGTT TTGGAAGGCT GTTTTAATGA TGGATTCAGA AAAAAGAATA 
AGATTACTTC AGTTTGTCAC TGGCACATCT CGGGTGCCTA TGAATGGATT TGCTGAACTA 
TACGGTTCAA ATGGACCACA GTCATTTACA GTTGAACAGT GGGGTACTCC TGAAAAGCTG 
CCAAGAGCTC ATACCTGTTT TAATCGCCTG GACTTGCCAC CTTATGAATC ATTTGAAGAA 
TTATGGGATA AACTTCAGAT GGCAATTGAA AACACCCAGG GCTTTGATGG AGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271267.1
CDS	301..4260
Translation	MAQSLRLHFAARRSNTYPLSETSGDDLDSHVHMCFKRPTRISTSNVVQMKLTPRQTALAPLIKENVQSQERSSVPSSENVNKKSSCLQISLQPTRYSGYLQSSNVLADSDDASFTCILKDGIYSSAVVDNELNAVNDGHLVSSPAICSGSLSNFSTSDNGSYSSNGSDFGSCASITSGGSYTNSVISDSSSYTFPPSDDTFLGGNLPSDSTSNRSVPNRNTTPCEIFSRSTSTDPFVQDDLEHGLEIMKLPVSRNTKIPLKRYSSLVIFPRSPSTTRPTSPTSLCTLLSKGSYQTSHQFIISPSEIAHNEDGTSAKGFLSTAVNGLRLSKTICTPGEVRDIRPLHRKGSLQKKIVLSNNTPRQTVCEKSSEGYSCVSVHFTQRKAATLDCETTNGDCKPEMSEIKLNSDSEYIKLMHRTSACLPSSQNVDCQININGELERPHSQMNKNHGILRRSISLGGAYPNISCLSSLKHNCSKGGPSQLLIKFASGNEGKVDNLSRDSNRDCTNELSNSCKTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	NM_001284338.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284338.1

ORF Insert Sequence:

ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA CCCTTTGTCA 
GAAACCTCCG GAGATGACTT GGATAGCCAT GTTCACATGT GCTTCAAAAG ACCAACACGG 
ATTTCAACGT CTAACGTTGT TCAAATGAAG CTGACTCCCA GACAGACTGC ACTAGCTCCG 
TTAATAAAGG AAAACGTTCA GTCTCAAGAA AGATCATCTG TTCCCTCATC TGAAAATGTT 
AATAAAAAGA GCAGCTGTCT ACAGATTTCA CTACAGCCAA CAAGGTACAG TGGATATCTT 
CAGTCTAGCA ATGTCTTAGC TGATAGTGAT GATGCTTCGT TTACTTGTAT CTTGAAGGAT 
GGTATTTACA GTAGTGCTGT GGTCGATAAT GAATTGAATG CTGTGAATGA TGGTCACCTT 
GTAAGCAGTC CAGCCATTTG TAGTGGTAGC CTTAGTAACT TTTCAACCAG TGATAATGGG 
TCTTACAGCA GCAACGGTAG TGATTTTGGG TCATGTGCAA GTATCACAAG TGGAGGTTCA 
TATACTAACA GTGTCATCAG TGACAGTAGT AGTTATACTT TTCCACCAAG TGATGATACT 
TTTTTGGGTG GAAACTTACC TTCTGACAGC ACCTCCAATA GAAGTGTGCC AAACAGGAAT 
ACTACTCCTT GTGAAATTTT TTCAAGAAGT ACAAGTACAG ATCCTTTTGT CCAGGATGAC 
TTGGAACATG GATTAGAGAT TATGAAATTG CCAGTGAGCA GGAACACAAA AATTCCACTA 
AAACGTTACT CCTCCTTAGT CATTTTTCCT AGGAGTCCTT CAACTACCCG ACCGACTTCT 
CCAACAAGTC TGTGTACTCT TCTGAGCAAA GGATCCTATC AAACTTCACA CCAGTTTATT 
ATTTCTCCTA GTGAAATTGC ACATAATGAG GATGGCACTA GTGCTAAAGG ATTTCTTTCA 
ACAGCTGTCA ATGGACTTCG GTTATCTAAA ACAATTTGTA CCCCCGGAGA AGTAAGAGAC 
ATACGGCCGC TTCACAGGAA GGGCTCGTTA CAGAAGAAAA TTGTTCTTTC GAATAATACT 
CCCAGACAGA CTGTCTGTGA AAAGTCATCT GAAGGATATT CTTGTGTTTC AGTGCATTTC 
ACCCAACGAA AAGCAGCTAC ATTAGACTGT GAAACAACAA ATGGTGATTG TAAACCAGAA 
ATGTCAGAAA TTAAGCTTAA TTCTGATTCA GAGTATATTA AGCTCATGCA TAGGACATCT 
GCATGTTTGC CATCCTCCCA AAATGTAGAT TGTCAAATAA ATATCAATGG AGAATTGGAA 
AGACCACATT CACAGATGAA CAAAAACCAT GGTATTTTAC GAAGAAGTAT TTCATTGGGA 
GGAGCTTATC CAAATATTTC CTGTCTATCC AGCCTTAAGC ACAATTGTTC TAAAGGGGGA 
CCATCTCAGT TACTCATAAA GTTTGCATCT GGAAATGAAG GTAAAGTGGA TAATTTATCA 
AGAGACAGCA ACAGAGATTG CACAAATGAA CTGTCTAATT CTTGCAAGAC AAGAGATGAT 
TTCCTAGGTC AAGTGGATGT TCCACTTTAT CCATTACCGA CAGAAAATCC AAGATTGGAG 
AGACCATATA CATTTAAGGA TTTTGTTCTT CATCCAAGAA GTCACAAATC AAGAGTTAAA 
GGTTATCTGA GACTAAAAAT GACTTATTTA CCTAAAACCA GTGGCTCAGA AGATGATAAT 
GCAGAACAGG CTGAGGAATT AGAGCCTGGC TGGGTTGTTT TGGACCAACC AGATGCTGCT 
TGCCATTTGC AGCAACAACA AGAACCTTCT CCTCTACCTC CAGGGTGGGA AGAGAGGCAG 
GATATCCTTG GAAGGACCTA TTATGTAAAC CATGAATCTA GAAGAACACA GTGGAAAAGA 
CCAACCCCTC AGGACAACCT AACAGATGCT GAGAATGGCA ACATTCAACT GCAAGCACAA 
CGTGCATTTA CCACCAGGCG GCAGATATCC GAGGAAACAG AAAGTGTTGA CAACCGAGAG 
TCTTCCGAGA ACTGGGAAAT TATAAGAGAA GATGAAGCCA CCATGTATAG CAACCAGGCC 
TTCCCATCAC CTCCACCGTC AAGTAACTTG GATGTTCCAA CTCATCTTGC AGAAGAATTG 
AATGCCAGAC TCACCATTTT TGGAAATTCA GCCGTGAGCC AGCCAGCATC GAGCTCAAAT 
CATTCCAGCA GAAGAGGCAG CTTACAAGCC TATACTTTTG AGGAACAACC TACACTTCCT 
GTGCTTTTGC CTACTTCATC TGGATTACCA CCAGGTTGGG AAGAAAAACA AGATGAAAGA 
GGAAGATCAT ATTATGTAGA TCACAATTCC AGAACGACTA CTTGGACAAA GCCCACTGTA 
CAGGCCACAG TGGAGACCAG TCAGCTGACC TCAAGCCAGA GTTCTGCAGG CCCTCAATCA 
CAAGCCTCCA CCAGTGATTC AGGCCAGCAG GTGACCCAGC CATCTGAAAT TGAGCAAGGA 
TTCCTTCCTA AAGGCTGGGA AGTCCGGCAT GCACCAAATG GGAGGCCTTT CTTTATTGAC 
CACAACACTA AAACCACCAC CTGGGAAGAT CCAAGATTGA AAATTCCAGC CCATCTGAGA 
GGAAAGACAT CACTTGATAC TTCCAATGAT CTAGGGCCTT TACCTCCAGG ATGGGAAGAG 
AGAACTCACA CAGATGGAAG AATCTTCTAC ATAAATCACA ATATAAAAAG AACACAATGG 
GAAGATCCTC GGTTGGAGAA TGTAGCAATA ACTGGACCAG CAGTGCCCTA CTCCAGGGAT 
TACAAAAGAA AGTATGAGTT CTTCCGAAGA AAGTTGAAGA AGCAGAATGA CATTCCAAAC 
AAATTTGAAA TGAAACTTCG CCGAGCAACT GTTCTTGAAG ACTCTTACCG GAGAATTATG 
GGTGTCAAGA GAGCAGACTT CCTGAAGGCT CGACTGTGGA TTGAGTTTGA TGGTGAAAAG 
GGATTGGATT ATGGAGGAGT TGCCAGAGAA TGGTTCTTCC TGATCTCAAA GGAAATGTTT 
AACCCTTATT ATGGGTTGTT TGAATATTCT GCTACGGACA ATTATACCCT ACAGATAAAT 
CCAAACTCTG GATTGTGTAA CGAAGATCAC CTCTCTTACT TCAAGTTTAT TGGTCGGGTA 
GCTGGAATGG CAGTTTATCA TGGCAAACTG TTGGATGGTT TTTTCATCCG CCCATTTTAC 
AAGATGATGC TTCACAAACC AATAACCCTT CATGATATGG AATCTGTGGA TAGTGAATAT 
TACAATTCCC TAAGATGGAT TCTTGAAAAT GACCCAACAG AATTGGACCT CAGGTTTATC 
ATAGATGAAG AACTTTTTGG ACAGACACAT CAACATGAGC TGAAAAATGG TGGATCAGAA 
ATAGTTGTCA CCAATAAGAA CAAAAAGGAA TATATTTATC TTGTAATACA ATGGCGATTT 
GTAAACCGAA TCCAGAAGCA AATGGCTGCT TTTAAAGAGG GATTCTTTGA ACTAATACCA 
CAGGATCTCA TCAAAATTTT TGATGAAAAT GAACTAGAGC TTCTTATGTG TGGACTGGGA 
GATGTTGATG TGAATGACTG GAGGGAACAT ACAAAGTATA AAAATGGCTA CAGTGCAAAT 
CATCAGGTTA TACAGTGGTT TTGGAAGGCT GTTTTAATGA TGGATTCAGA AAAAAGAATA 
AGATTACTTC AGTTTGTCAC TGGCACATCT CGGGTGCCTA TGAATGGATT TGCTGAACTA 
TACGGTTCAA ATGGACCACA GTCATTTACA GTTGAACAGT GGGGTACTCC TGAAAAGCTG 
CCAAGAGCTC ATACCTGTTT TAATCGCCTG GACTTGCCAC CTTATGAATC ATTTGAAGAA 
TTATGGGATA AACTTCAGAT GGCAATTGAA AACACCCAGG GCTTTGATGG AGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( NM_001329212.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316141.1 Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu100779
Clone ID Related Accession (Same CDS sequence)	NM_001329212.2 , NM_001329212.1
Accession Version	NM_001329212.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D42055.1, AC039057.8, AL832063.1, AL833300.1 and AC009997.7. On Jun 2, 2019 this sequence version replaced NM_001329212.1. Transcript Variant: This variant (7) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains multiple exons in the 5' UTR, compared to variant 2. It represents use of an alternate promoter and initiates translation at an alternate start codon. The encoded isoform (6) is shorter and has a distinct N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.184063.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTTATT TACCTAAAAC CAGTGGCTCA GAAGATGATA ATGCAGAACA GGCTGAGGAA 
TTAGAGCCTG GCTGGGTTGT TTTGGACCAA CCAGATGCTG CTTGCCATTT GCAGCAACAA 
CAAGAACCTT CTCCTCTACC TCCAGGGTGG GAAGAGAGGC AGGATATCCT TGGAAGGACC 
TATTATGTAA ACCATGAATC TAGAAGAACA CAGTGGAAAA GACCAACCCC TCAGGACAAC 
CTAACAGATG CTGAGAATGG CAACATTCAA CTGCAAGCAC AACGTGCATT TACCACCAGG 
CGGCAGATAT CCGAGGAAAC AGAAAGTGTT GACAACCGAG AGTCTTCCGA GAACTGGGAA 
ATTATAAGAG AAGATGAAGC CACCATGTAT AGCAACCAGG CCTTCCCATC ACCTCCACCG 
TCAAGTAACT TGGATGTTCC AACTCATCTT GCAGAAGAAT TGAATGCCAG ACTCACCATT 
TTTGGAAATT CAGCCGTGAG CCAGCCAGCA TCGAGCTCAA ATCATTCCAG CAGAAGAGGC 
AGCTTACAAG CCTATACTTT TGAGGAACAA CCTACACTTC CTGTGCTTTT GCCTACTTCA 
TCTGGATTAC CACCAGGTTG GGAAGAAAAA CAAGATGAAA GAGGAAGATC ATATTATGTA 
GATCACAATT CCAGAACGAC TACTTGGACA AAGCCCACTG TACAGGCCAC AGTGGAGACC 
AGTCAGCTGA CCTCAAGCCA GAGTTCTGCA GGCCCTCAAT CACAAGCCTC CACCAGTGAT 
TCAGGCCAGC AGGTGACCCA GCCATCTGAA ATTGAGCAAG GATTCCTTCC TAAAGGCTGG 
GAAGTCCGGC ATGCACCAAA TGGGAGGCCT TTCTTTATTG ACCACAACAC TAAAACCACC 
ACCTGGGAAG ATCCAAGATT GAAAATTCCA GCCCATCTGA GAGGAAAGAC ATCACTTGAT 
ACTTCCAATG ATCTAGGGCC TTTACCTCCA GGATGGGAAG AGAGAACTCA CACAGATGGA 
AGAATCTTCT ACATAAATCA CAATATAAAA AGAACACAAT GGGAAGATCC TCGGTTGGAG 
AATGTAGCAA TAACTGGACC AGCAGTGCCC TACTCCAGGG ATTACAAAAG AAAGTATGAG 
TTCTTCCGAA GAAAGTTGAA GAAGCAGAAT GACATTCCAA ACAAATTTGA AATGAAACTT 
CGCCGAGCAA CTGTTCTTGA AGACTCTTAC CGGAGAATTA TGGGTGTCAA GAGAGCAGAC 
TTCCTGAAGG CTCGACTGTG GATTGAGTTT GATGGTGAAA AGGGATTGGA TTATGGAGGA 
GTTGCCAGAG AATGGTTCTT CCTGATCTCA AAGGAAATGT TTAACCCTTA TTATGGGTTG 
TTTGAATATT CTGCTACGGA CAATTATACC CTACAGATAA ATCCAAACTC TGGATTGTGT 
AACGAAGATC ACCTCTCTTA CTTCAAGTTT ATTGGTCGGG TAGCTGGAAT GGCAGTTTAT 
CATGGCAAAC TGTTGGATGG TTTTTTCATC CGCCCATTTT ACAAGATGAT GCTTCACAAA 
CCAATAACCC TTCATGATAT GGAATCTGTG GATAGTGAAT ATTACAATTC CCTAAGATGG 
ATTCTTGAAA ATGACCCAAC AGAATTGGAC CTCAGGTTTA TCATAGATGA AGAACTTTTT 
GGACAGACAC ATCAACATGA GCTGAAAAAT GGTGGATCAG AAATAGTTGT CACCAATAAG 
AACAAAAAGG AATATATTTA TCTTGTAATA CAATGGCGAT TTGTAAACCG AATCCAGAAG 
CAAATGGCTG CTTTTAAAGA GGGATTCTTT GAACTAATAC CACAGGATCT CATCAAAATT 
TTTGATGAAA ATGAACTAGA GCTTCTTATG TGTGGACTGG GAGATGTTGA TGTGAATGAC 
TGGAGGGAAC ATACAAAGTA TAAAAATGGC TACAGTGCAA ATCATCAGGT TATACAGTGG 
TTTTGGAAGG CTGTTTTAAT GATGGATTCA GAAAAAAGAA TAAGATTACT TCAGTTTGTC 
ACTGGCACAT CTCGGGTGCC TATGAATGGA TTTGCTGAAC TATACGGTTC AAATGGACCA 
CAGTCATTTA CAGTTGAACA GTGGGGTACT CCTGAAAAGC TGCCAAGAGC TCATACCTGT 
TTTAATCGCC TGGACTTGCC ACCTTATGAA TCATTTGAAG AATTATGGGA TAAACTTCAG 
ATGGCAATTG AAAACACCCA GGGCTTTGAT GGAGTTGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316141.1
CDS	524..2785
Translation	MTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	NM_001329212.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329212.2

ORF Insert Sequence:

ATGACTTATT TACCTAAAAC CAGTGGCTCA GAAGATGATA ATGCAGAACA GGCTGAGGAA 
TTAGAGCCTG GCTGGGTTGT TTTGGACCAA CCAGATGCTG CTTGCCATTT GCAGCAACAA 
CAAGAACCTT CTCCTCTACC TCCAGGGTGG GAAGAGAGGC AGGATATCCT TGGAAGGACC 
TATTATGTAA ACCATGAATC TAGAAGAACA CAGTGGAAAA GACCAACCCC TCAGGACAAC 
CTAACAGATG CTGAGAATGG CAACATTCAA CTGCAAGCAC AACGTGCATT TACCACCAGG 
CGGCAGATAT CCGAGGAAAC AGAAAGTGTT GACAACCGAG AGTCTTCCGA GAACTGGGAA 
ATTATAAGAG AAGATGAAGC CACCATGTAT AGCAACCAGG CCTTCCCATC ACCTCCACCG 
TCAAGTAACT TGGATGTTCC AACTCATCTT GCAGAAGAAT TGAATGCCAG ACTCACCATT 
TTTGGAAATT CAGCCGTGAG CCAGCCAGCA TCGAGCTCAA ATCATTCCAG CAGAAGAGGC 
AGCTTACAAG CCTATACTTT TGAGGAACAA CCTACACTTC CTGTGCTTTT GCCTACTTCA 
TCTGGATTAC CACCAGGTTG GGAAGAAAAA CAAGATGAAA GAGGAAGATC ATATTATGTA 
GATCACAATT CCAGAACGAC TACTTGGACA AAGCCCACTG TACAGGCCAC AGTGGAGACC 
AGTCAGCTGA CCTCAAGCCA GAGTTCTGCA GGCCCTCAAT CACAAGCCTC CACCAGTGAT 
TCAGGCCAGC AGGTGACCCA GCCATCTGAA ATTGAGCAAG GATTCCTTCC TAAAGGCTGG 
GAAGTCCGGC ATGCACCAAA TGGGAGGCCT TTCTTTATTG ACCACAACAC TAAAACCACC 
ACCTGGGAAG ATCCAAGATT GAAAATTCCA GCCCATCTGA GAGGAAAGAC ATCACTTGAT 
ACTTCCAATG ATCTAGGGCC TTTACCTCCA GGATGGGAAG AGAGAACTCA CACAGATGGA 
AGAATCTTCT ACATAAATCA CAATATAAAA AGAACACAAT GGGAAGATCC TCGGTTGGAG 
AATGTAGCAA TAACTGGACC AGCAGTGCCC TACTCCAGGG ATTACAAAAG AAAGTATGAG 
TTCTTCCGAA GAAAGTTGAA GAAGCAGAAT GACATTCCAA ACAAATTTGA AATGAAACTT 
CGCCGAGCAA CTGTTCTTGA AGACTCTTAC CGGAGAATTA TGGGTGTCAA GAGAGCAGAC 
TTCCTGAAGG CTCGACTGTG GATTGAGTTT GATGGTGAAA AGGGATTGGA TTATGGAGGA 
GTTGCCAGAG AATGGTTCTT CCTGATCTCA AAGGAAATGT TTAACCCTTA TTATGGGTTG 
TTTGAATATT CTGCTACGGA CAATTATACC CTACAGATAA ATCCAAACTC TGGATTGTGT 
AACGAAGATC ACCTCTCTTA CTTCAAGTTT ATTGGTCGGG TAGCTGGAAT GGCAGTTTAT 
CATGGCAAAC TGTTGGATGG TTTTTTCATC CGCCCATTTT ACAAGATGAT GCTTCACAAA 
CCAATAACCC TTCATGATAT GGAATCTGTG GATAGTGAAT ATTACAATTC CCTAAGATGG 
ATTCTTGAAA ATGACCCAAC AGAATTGGAC CTCAGGTTTA TCATAGATGA AGAACTTTTT 
GGACAGACAC ATCAACATGA GCTGAAAAAT GGTGGATCAG AAATAGTTGT CACCAATAAG 
AACAAAAAGG AATATATTTA TCTTGTAATA CAATGGCGAT TTGTAAACCG AATCCAGAAG 
CAAATGGCTG CTTTTAAAGA GGGATTCTTT GAACTAATAC CACAGGATCT CATCAAAATT 
TTTGATGAAA ATGAACTAGA GCTTCTTATG TGTGGACTGG GAGATGTTGA TGTGAATGAC 
TGGAGGGAAC ATACAAAGTA TAAAAATGGC TACAGTGCAA ATCATCAGGT TATACAGTGG 
TTTTGGAAGG CTGTTTTAAT GATGGATTCA GAAAAAAGAA TAAGATTACT TCAGTTTGTC 
ACTGGCACAT CTCGGGTGCC TATGAATGGA TTTGCTGAAC TATACGGTTC AAATGGACCA 
CAGTCATTTA CAGTTGAACA GTGGGGTACT CCTGAAAAGC TGCCAAGAGC TCATACCTGT 
TTTAATCGCC TGGACTTGCC ACCTTATGAA TCATTTGAAG AATTATGGGA TAAACTTCAG 
ATGGCAATTG AAAACACCCA GGGCTTTGAT GGAGTTGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( NM_001329212.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316141.1 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu100779
Clone ID Related Accession (Same CDS sequence)	NM_001329212.2 , NM_001329212.1
Accession Version	NM_001329212.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB451823.1, D42055.1, AC039057.8, AL832063.1, AL833300.1 and AC009997.7. Transcript Variant: This variant (7) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains multiple exons in the 5' UTR, compared to variant 2. It represents use of an alternate promoter and initiates translation at an alternate start codon. The encoded isoform (6) is shorter and has a distinct N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.184063.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTTATT TACCTAAAAC CAGTGGCTCA GAAGATGATA ATGCAGAACA GGCTGAGGAA 
TTAGAGCCTG GCTGGGTTGT TTTGGACCAA CCAGATGCTG CTTGCCATTT GCAGCAACAA 
CAAGAACCTT CTCCTCTACC TCCAGGGTGG GAAGAGAGGC AGGATATCCT TGGAAGGACC 
TATTATGTAA ACCATGAATC TAGAAGAACA CAGTGGAAAA GACCAACCCC TCAGGACAAC 
CTAACAGATG CTGAGAATGG CAACATTCAA CTGCAAGCAC AACGTGCATT TACCACCAGG 
CGGCAGATAT CCGAGGAAAC AGAAAGTGTT GACAACCGAG AGTCTTCCGA GAACTGGGAA 
ATTATAAGAG AAGATGAAGC CACCATGTAT AGCAACCAGG CCTTCCCATC ACCTCCACCG 
TCAAGTAACT TGGATGTTCC AACTCATCTT GCAGAAGAAT TGAATGCCAG ACTCACCATT 
TTTGGAAATT CAGCCGTGAG CCAGCCAGCA TCGAGCTCAA ATCATTCCAG CAGAAGAGGC 
AGCTTACAAG CCTATACTTT TGAGGAACAA CCTACACTTC CTGTGCTTTT GCCTACTTCA 
TCTGGATTAC CACCAGGTTG GGAAGAAAAA CAAGATGAAA GAGGAAGATC ATATTATGTA 
GATCACAATT CCAGAACGAC TACTTGGACA AAGCCCACTG TACAGGCCAC AGTGGAGACC 
AGTCAGCTGA CCTCAAGCCA GAGTTCTGCA GGCCCTCAAT CACAAGCCTC CACCAGTGAT 
TCAGGCCAGC AGGTGACCCA GCCATCTGAA ATTGAGCAAG GATTCCTTCC TAAAGGCTGG 
GAAGTCCGGC ATGCACCAAA TGGGAGGCCT TTCTTTATTG ACCACAACAC TAAAACCACC 
ACCTGGGAAG ATCCAAGATT GAAAATTCCA GCCCATCTGA GAGGAAAGAC ATCACTTGAT 
ACTTCCAATG ATCTAGGGCC TTTACCTCCA GGATGGGAAG AGAGAACTCA CACAGATGGA 
AGAATCTTCT ACATAAATCA CAATATAAAA AGAACACAAT GGGAAGATCC TCGGTTGGAG 
AATGTAGCAA TAACTGGACC AGCAGTGCCC TACTCCAGGG ATTACAAAAG AAAGTATGAG 
TTCTTCCGAA GAAAGTTGAA GAAGCAGAAT GACATTCCAA ACAAATTTGA AATGAAACTT 
CGCCGAGCAA CTGTTCTTGA AGACTCTTAC CGGAGAATTA TGGGTGTCAA GAGAGCAGAC 
TTCCTGAAGG CTCGACTGTG GATTGAGTTT GATGGTGAAA AGGGATTGGA TTATGGAGGA 
GTTGCCAGAG AATGGTTCTT CCTGATCTCA AAGGAAATGT TTAACCCTTA TTATGGGTTG 
TTTGAATATT CTGCTACGGA CAATTATACC CTACAGATAA ATCCAAACTC TGGATTGTGT 
AACGAAGATC ACCTCTCTTA CTTCAAGTTT ATTGGTCGGG TAGCTGGAAT GGCAGTTTAT 
CATGGCAAAC TGTTGGATGG TTTTTTCATC CGCCCATTTT ACAAGATGAT GCTTCACAAA 
CCAATAACCC TTCATGATAT GGAATCTGTG GATAGTGAAT ATTACAATTC CCTAAGATGG 
ATTCTTGAAA ATGACCCAAC AGAATTGGAC CTCAGGTTTA TCATAGATGA AGAACTTTTT 
GGACAGACAC ATCAACATGA GCTGAAAAAT GGTGGATCAG AAATAGTTGT CACCAATAAG 
AACAAAAAGG AATATATTTA TCTTGTAATA CAATGGCGAT TTGTAAACCG AATCCAGAAG 
CAAATGGCTG CTTTTAAAGA GGGATTCTTT GAACTAATAC CACAGGATCT CATCAAAATT 
TTTGATGAAA ATGAACTAGA GCTTCTTATG TGTGGACTGG GAGATGTTGA TGTGAATGAC 
TGGAGGGAAC ATACAAAGTA TAAAAATGGC TACAGTGCAA ATCATCAGGT TATACAGTGG 
TTTTGGAAGG CTGTTTTAAT GATGGATTCA GAAAAAAGAA TAAGATTACT TCAGTTTGTC 
ACTGGCACAT CTCGGGTGCC TATGAATGGA TTTGCTGAAC TATACGGTTC AAATGGACCA 
CAGTCATTTA CAGTTGAACA GTGGGGTACT CCTGAAAAGC TGCCAAGAGC TCATACCTGT 
TTTAATCGCC TGGACTTGCC ACCTTATGAA TCATTTGAAG AATTATGGGA TAAACTTCAG 
ATGGCAATTG AAAACACCCA GGGCTTTGAT GGAGTTGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316141.1
CDS	658..2919
Translation	MTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	NM_001329212.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329212.1

ORF Insert Sequence:

ATGACTTATT TACCTAAAAC CAGTGGCTCA GAAGATGATA ATGCAGAACA GGCTGAGGAA 
TTAGAGCCTG GCTGGGTTGT TTTGGACCAA CCAGATGCTG CTTGCCATTT GCAGCAACAA 
CAAGAACCTT CTCCTCTACC TCCAGGGTGG GAAGAGAGGC AGGATATCCT TGGAAGGACC 
TATTATGTAA ACCATGAATC TAGAAGAACA CAGTGGAAAA GACCAACCCC TCAGGACAAC 
CTAACAGATG CTGAGAATGG CAACATTCAA CTGCAAGCAC AACGTGCATT TACCACCAGG 
CGGCAGATAT CCGAGGAAAC AGAAAGTGTT GACAACCGAG AGTCTTCCGA GAACTGGGAA 
ATTATAAGAG AAGATGAAGC CACCATGTAT AGCAACCAGG CCTTCCCATC ACCTCCACCG 
TCAAGTAACT TGGATGTTCC AACTCATCTT GCAGAAGAAT TGAATGCCAG ACTCACCATT 
TTTGGAAATT CAGCCGTGAG CCAGCCAGCA TCGAGCTCAA ATCATTCCAG CAGAAGAGGC 
AGCTTACAAG CCTATACTTT TGAGGAACAA CCTACACTTC CTGTGCTTTT GCCTACTTCA 
TCTGGATTAC CACCAGGTTG GGAAGAAAAA CAAGATGAAA GAGGAAGATC ATATTATGTA 
GATCACAATT CCAGAACGAC TACTTGGACA AAGCCCACTG TACAGGCCAC AGTGGAGACC 
AGTCAGCTGA CCTCAAGCCA GAGTTCTGCA GGCCCTCAAT CACAAGCCTC CACCAGTGAT 
TCAGGCCAGC AGGTGACCCA GCCATCTGAA ATTGAGCAAG GATTCCTTCC TAAAGGCTGG 
GAAGTCCGGC ATGCACCAAA TGGGAGGCCT TTCTTTATTG ACCACAACAC TAAAACCACC 
ACCTGGGAAG ATCCAAGATT GAAAATTCCA GCCCATCTGA GAGGAAAGAC ATCACTTGAT 
ACTTCCAATG ATCTAGGGCC TTTACCTCCA GGATGGGAAG AGAGAACTCA CACAGATGGA 
AGAATCTTCT ACATAAATCA CAATATAAAA AGAACACAAT GGGAAGATCC TCGGTTGGAG 
AATGTAGCAA TAACTGGACC AGCAGTGCCC TACTCCAGGG ATTACAAAAG AAAGTATGAG 
TTCTTCCGAA GAAAGTTGAA GAAGCAGAAT GACATTCCAA ACAAATTTGA AATGAAACTT 
CGCCGAGCAA CTGTTCTTGA AGACTCTTAC CGGAGAATTA TGGGTGTCAA GAGAGCAGAC 
TTCCTGAAGG CTCGACTGTG GATTGAGTTT GATGGTGAAA AGGGATTGGA TTATGGAGGA 
GTTGCCAGAG AATGGTTCTT CCTGATCTCA AAGGAAATGT TTAACCCTTA TTATGGGTTG 
TTTGAATATT CTGCTACGGA CAATTATACC CTACAGATAA ATCCAAACTC TGGATTGTGT 
AACGAAGATC ACCTCTCTTA CTTCAAGTTT ATTGGTCGGG TAGCTGGAAT GGCAGTTTAT 
CATGGCAAAC TGTTGGATGG TTTTTTCATC CGCCCATTTT ACAAGATGAT GCTTCACAAA 
CCAATAACCC TTCATGATAT GGAATCTGTG GATAGTGAAT ATTACAATTC CCTAAGATGG 
ATTCTTGAAA ATGACCCAAC AGAATTGGAC CTCAGGTTTA TCATAGATGA AGAACTTTTT 
GGACAGACAC ATCAACATGA GCTGAAAAAT GGTGGATCAG AAATAGTTGT CACCAATAAG 
AACAAAAAGG AATATATTTA TCTTGTAATA CAATGGCGAT TTGTAAACCG AATCCAGAAG 
CAAATGGCTG CTTTTAAAGA GGGATTCTTT GAACTAATAC CACAGGATCT CATCAAAATT 
TTTGATGAAA ATGAACTAGA GCTTCTTATG TGTGGACTGG GAGATGTTGA TGTGAATGAC 
TGGAGGGAAC ATACAAAGTA TAAAAATGGC TACAGTGCAA ATCATCAGGT TATACAGTGG 
TTTTGGAAGG CTGTTTTAAT GATGGATTCA GAAAAAAGAA TAAGATTACT TCAGTTTGTC 
ACTGGCACAT CTCGGGTGCC TATGAATGGA TTTGCTGAAC TATACGGTTC AAATGGACCA 
CAGTCATTTA CAGTTGAACA GTGGGGTACT CCTGAAAAGC TGCCAAGAGC TCATACCTGT 
TTTAATCGCC TGGACTTGCC ACCTTATGAA TCATTTGAAG AATTATGGGA TAAACTTCAG 
ATGGCAATTG AAAACACCCA GGGCTTTGAT GGAGTTGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( NM_006154.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006145.2 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21031
Clone ID Related Accession (Same CDS sequence)	NM_006154.3 , NM_006154.4
Accession Version	NM_006154.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB451823.1, D42055.1, AL832063.1, AL833300.1 and AC009997.7. This sequence is a reference standard in the RefSeqGene project. On Sep 28, 2013 this sequence version replaced NM_006154.2. Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains multiple alternate 5' coding exons, compared to variant 2. It represents use of an alternate promoter and initiates translation at an alternate start codon. The encoded isoform (1) is shorter and has a distinct N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC152452.1, D42055.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAACTT GCGCGGTGGA GGTGTTCGGG CTCCTGGAGG ACGAGGAAAA TTCACGAATT 
GTGAGAGTAA GAGTTATAGC CGGAATAGGC CTTGCCAAGA AGGATATATT GGGAGCTAGT 
GATCCTTACG TGAGAGTGAC GTTATATGAC CCAATGAATG GAGTTCTTAC AAGTGTGCAA 
ACAAAAACCA TTAAAAAGAG TTTGAATCCA AAGTGGAATG AAGAAATATT ATTCAGAGTT 
CATCCTCAGC AGCACCGGCT TCTTTTTGAA GTGTTTGACG AAAACCGATT GACAAGAGAT 
GATTTCCTAG GTCAAGTGGA TGTTCCACTT TATCCATTAC CGACAGAAAA TCCAAGATTG 
GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
AAAGGTTATC TGAGACTAAA AATGACTTAT TTACCTAAAA CCAGTGGCTC AGAAGATGAT 
AATGCAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTTG TTTTGGACCA ACCAGATGCT 
GCTTGCCATT TGCAGCAACA ACAAGAACCT TCTCCTCTAC CTCCAGGGTG GGAAGAGAGG 
CAGGATATCC TTGGAAGGAC CTATTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
AGACCAACCC CTCAGGACAA CCTAACAGAT GCTGAGAATG GCAACATTCA ACTGCAAGCA 
CAACGTGCAT TTACCACCAG GCGGCAGATA TCCGAGGAAA CAGAAAGTGT TGACAACCGA 
GAGTCTTCCG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TAGCAACCAG 
GCCTTCCCAT CACCTCCACC GTCAAGTAAC TTGGATGTTC CAACTCATCT TGCAGAAGAA 
TTGAATGCCA GACTCACCAT TTTTGGAAAT TCAGCCGTGA GCCAGCCAGC ATCGAGCTCA 
AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA ACCTACACTT 
CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
AGAGGAAGAT CATATTATGT AGATCACAAT TCCAGAACGA CTACTTGGAC AAAGCCCACT 
GTACAGGCCA CAGTGGAGAC CAGTCAGCTG ACCTCAAGCC AGAGTTCTGC AGGCCCTCAA 
TCACAAGCCT CCACCAGTGA TTCAGGCCAG CAGGTGACCC AGCCATCTGA AATTGAGCAA 
GGATTCCTTC CTAAAGGCTG GGAAGTCCGG CATGCACCAA ATGGGAGGCC TTTCTTTATT 
GACCACAACA CTAAAACCAC CACCTGGGAA GATCCAAGAT TGAAAATTCC AGCCCATCTG 
AGAGGAAAGA CATCACTTGA TACTTCCAAT GATCTAGGGC CTTTACCTCC AGGATGGGAA 
GAGAGAACTC ACACAGATGG AAGAATCTTC TACATAAATC ACAATATAAA AAGAACACAA 
TGGGAAGATC CTCGGTTGGA GAATGTAGCA ATAACTGGAC CAGCAGTGCC CTACTCCAGG 
GATTACAAAA GAAAGTATGA GTTCTTCCGA AGAAAGTTGA AGAAGCAGAA TGACATTCCA 
AACAAATTTG AAATGAAACT TCGCCGAGCA ACTGTTCTTG AAGACTCTTA CCGGAGAATT 
ATGGGTGTCA AGAGAGCAGA CTTCCTGAAG GCTCGACTGT GGATTGAGTT TGATGGTGAA 
AAGGGATTGG ATTATGGAGG AGTTGCCAGA GAATGGTTCT TCCTGATCTC AAAGGAAATG 
TTTAACCCTT ATTATGGGTT GTTTGAATAT TCTGCTACGG ACAATTATAC CCTACAGATA 
AATCCAAACT CTGGATTGTG TAACGAAGAT CACCTCTCTT ACTTCAAGTT TATTGGTCGG 
GTAGCTGGAA TGGCAGTTTA TCATGGCAAA CTGTTGGATG GTTTTTTCAT CCGCCCATTT 
TACAAGATGA TGCTTCACAA ACCAATAACC CTTCATGATA TGGAATCTGT GGATAGTGAA 
TATTACAATT CCCTAAGATG GATTCTTGAA AATGACCCAA CAGAATTGGA CCTCAGGTTT 
ATCATAGATG AAGAACTTTT TGGACAGACA CATCAACATG AGCTGAAAAA TGGTGGATCA 
GAAATAGTTG TCACCAATAA GAACAAAAAG GAATATATTT ATCTTGTAAT ACAATGGCGA 
TTTGTAAACC GAATCCAGAA GCAAATGGCT GCTTTTAAAG AGGGATTCTT TGAACTAATA 
CCACAGGATC TCATCAAAAT TTTTGATGAA AATGAACTAG AGCTTCTTAT GTGTGGACTG 
GGAGATGTTG ATGTGAATGA CTGGAGGGAA CATACAAAGT ATAAAAATGG CTACAGTGCA 
AATCATCAGG TTATACAGTG GTTTTGGAAG GCTGTTTTAA TGATGGATTC AGAAAAAAGA 
ATAAGATTAC TTCAGTTTGT CACTGGCACA TCTCGGGTGC CTATGAATGG ATTTGCTGAA 
CTATACGGTT CAAATGGACC ACAGTCATTT ACAGTTGAAC AGTGGGGTAC TCCTGAAAAG 
CTGCCAAGAG CTCATACCTG TTTTAATCGC CTGGACTTGC CACCTTATGA ATCATTTGAA 
GAATTATGGG ATAAACTTCA GATGGCAATT GAAAACACCC AGGGCTTTGA TGGAGTTGAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006145.2
CDS	192..2894
Translation	MATCAVEVFGLLEDEENSRIVRVRVIAGIGLAKKDILGASDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	NM_006154.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006154.3

ORF Insert Sequence:

ATGGCAACTT GCGCGGTGGA GGTGTTCGGG CTCCTGGAGG ACGAGGAAAA TTCACGAATT 
GTGAGAGTAA GAGTTATAGC CGGAATAGGC CTTGCCAAGA AGGATATATT GGGAGCTAGT 
GATCCTTACG TGAGAGTGAC GTTATATGAC CCAATGAATG GAGTTCTTAC AAGTGTGCAA 
ACAAAAACCA TTAAAAAGAG TTTGAATCCA AAGTGGAATG AAGAAATATT ATTCAGAGTT 
CATCCTCAGC AGCACCGGCT TCTTTTTGAA GTGTTTGACG AAAACCGATT GACAAGAGAT 
GATTTCCTAG GTCAAGTGGA TGTTCCACTT TATCCATTAC CGACAGAAAA TCCAAGATTG 
GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
AAAGGTTATC TGAGACTAAA AATGACTTAT TTACCTAAAA CCAGTGGCTC AGAAGATGAT 
AATGCAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTTG TTTTGGACCA ACCAGATGCT 
GCTTGCCATT TGCAGCAACA ACAAGAACCT TCTCCTCTAC CTCCAGGGTG GGAAGAGAGG 
CAGGATATCC TTGGAAGGAC CTATTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
AGACCAACCC CTCAGGACAA CCTAACAGAT GCTGAGAATG GCAACATTCA ACTGCAAGCA 
CAACGTGCAT TTACCACCAG GCGGCAGATA TCCGAGGAAA CAGAAAGTGT TGACAACCGA 
GAGTCTTCCG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TAGCAACCAG 
GCCTTCCCAT CACCTCCACC GTCAAGTAAC TTGGATGTTC CAACTCATCT TGCAGAAGAA 
TTGAATGCCA GACTCACCAT TTTTGGAAAT TCAGCCGTGA GCCAGCCAGC ATCGAGCTCA 
AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA ACCTACACTT 
CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
AGAGGAAGAT CATATTATGT AGATCACAAT TCCAGAACGA CTACTTGGAC AAAGCCCACT 
GTACAGGCCA CAGTGGAGAC CAGTCAGCTG ACCTCAAGCC AGAGTTCTGC AGGCCCTCAA 
TCACAAGCCT CCACCAGTGA TTCAGGCCAG CAGGTGACCC AGCCATCTGA AATTGAGCAA 
GGATTCCTTC CTAAAGGCTG GGAAGTCCGG CATGCACCAA ATGGGAGGCC TTTCTTTATT 
GACCACAACA CTAAAACCAC CACCTGGGAA GATCCAAGAT TGAAAATTCC AGCCCATCTG 
AGAGGAAAGA CATCACTTGA TACTTCCAAT GATCTAGGGC CTTTACCTCC AGGATGGGAA 
GAGAGAACTC ACACAGATGG AAGAATCTTC TACATAAATC ACAATATAAA AAGAACACAA 
TGGGAAGATC CTCGGTTGGA GAATGTAGCA ATAACTGGAC CAGCAGTGCC CTACTCCAGG 
GATTACAAAA GAAAGTATGA GTTCTTCCGA AGAAAGTTGA AGAAGCAGAA TGACATTCCA 
AACAAATTTG AAATGAAACT TCGCCGAGCA ACTGTTCTTG AAGACTCTTA CCGGAGAATT 
ATGGGTGTCA AGAGAGCAGA CTTCCTGAAG GCTCGACTGT GGATTGAGTT TGATGGTGAA 
AAGGGATTGG ATTATGGAGG AGTTGCCAGA GAATGGTTCT TCCTGATCTC AAAGGAAATG 
TTTAACCCTT ATTATGGGTT GTTTGAATAT TCTGCTACGG ACAATTATAC CCTACAGATA 
AATCCAAACT CTGGATTGTG TAACGAAGAT CACCTCTCTT ACTTCAAGTT TATTGGTCGG 
GTAGCTGGAA TGGCAGTTTA TCATGGCAAA CTGTTGGATG GTTTTTTCAT CCGCCCATTT 
TACAAGATGA TGCTTCACAA ACCAATAACC CTTCATGATA TGGAATCTGT GGATAGTGAA 
TATTACAATT CCCTAAGATG GATTCTTGAA AATGACCCAA CAGAATTGGA CCTCAGGTTT 
ATCATAGATG AAGAACTTTT TGGACAGACA CATCAACATG AGCTGAAAAA TGGTGGATCA 
GAAATAGTTG TCACCAATAA GAACAAAAAG GAATATATTT ATCTTGTAAT ACAATGGCGA 
TTTGTAAACC GAATCCAGAA GCAAATGGCT GCTTTTAAAG AGGGATTCTT TGAACTAATA 
CCACAGGATC TCATCAAAAT TTTTGATGAA AATGAACTAG AGCTTCTTAT GTGTGGACTG 
GGAGATGTTG ATGTGAATGA CTGGAGGGAA CATACAAAGT ATAAAAATGG CTACAGTGCA 
AATCATCAGG TTATACAGTG GTTTTGGAAG GCTGTTTTAA TGATGGATTC AGAAAAAAGA 
ATAAGATTAC TTCAGTTTGT CACTGGCACA TCTCGGGTGC CTATGAATGG ATTTGCTGAA 
CTATACGGTT CAAATGGACC ACAGTCATTT ACAGTTGAAC AGTGGGGTAC TCCTGAAAAG 
CTGCCAAGAG CTCATACCTG TTTTAATCGC CTGGACTTGC CACCTTATGA ATCATTTGAA 
GAATTATGGG ATAAACTTCA GATGGCAATT GAAAACACCC AGGGCTTTGA TGGAGTTGAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( NM_006154.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006145.2 Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 1, mRNA.

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CloneID	OHu21031
Clone ID Related Accession (Same CDS sequence)	NM_006154.3 , NM_006154.4
Accession Version	NM_006154.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D42055.1, AL832063.1, AL833300.1 and AC009997.7. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_006154.3. Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains multiple alternate 5' coding exons, compared to variant 2. It represents use of an alternate promoter and initiates translation at an alternate start codon. The encoded isoform (1) is shorter and has a distinct N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC152562.1, D42055.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAACTT GCGCGGTGGA GGTGTTCGGG CTCCTGGAGG ACGAGGAAAA TTCACGAATT 
GTGAGAGTAA GAGTTATAGC CGGAATAGGC CTTGCCAAGA AGGATATATT GGGAGCTAGT 
GATCCTTACG TGAGAGTGAC GTTATATGAC CCAATGAATG GAGTTCTTAC AAGTGTGCAA 
ACAAAAACCA TTAAAAAGAG TTTGAATCCA AAGTGGAATG AAGAAATATT ATTCAGAGTT 
CATCCTCAGC AGCACCGGCT TCTTTTTGAA GTGTTTGACG AAAACCGATT GACAAGAGAT 
GATTTCCTAG GTCAAGTGGA TGTTCCACTT TATCCATTAC CGACAGAAAA TCCAAGATTG 
GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
AAAGGTTATC TGAGACTAAA AATGACTTAT TTACCTAAAA CCAGTGGCTC AGAAGATGAT 
AATGCAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTTG TTTTGGACCA ACCAGATGCT 
GCTTGCCATT TGCAGCAACA ACAAGAACCT TCTCCTCTAC CTCCAGGGTG GGAAGAGAGG 
CAGGATATCC TTGGAAGGAC CTATTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
AGACCAACCC CTCAGGACAA CCTAACAGAT GCTGAGAATG GCAACATTCA ACTGCAAGCA 
CAACGTGCAT TTACCACCAG GCGGCAGATA TCCGAGGAAA CAGAAAGTGT TGACAACCGA 
GAGTCTTCCG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TAGCAACCAG 
GCCTTCCCAT CACCTCCACC GTCAAGTAAC TTGGATGTTC CAACTCATCT TGCAGAAGAA 
TTGAATGCCA GACTCACCAT TTTTGGAAAT TCAGCCGTGA GCCAGCCAGC ATCGAGCTCA 
AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA ACCTACACTT 
CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
AGAGGAAGAT CATATTATGT AGATCACAAT TCCAGAACGA CTACTTGGAC AAAGCCCACT 
GTACAGGCCA CAGTGGAGAC CAGTCAGCTG ACCTCAAGCC AGAGTTCTGC AGGCCCTCAA 
TCACAAGCCT CCACCAGTGA TTCAGGCCAG CAGGTGACCC AGCCATCTGA AATTGAGCAA 
GGATTCCTTC CTAAAGGCTG GGAAGTCCGG CATGCACCAA ATGGGAGGCC TTTCTTTATT 
GACCACAACA CTAAAACCAC CACCTGGGAA GATCCAAGAT TGAAAATTCC AGCCCATCTG 
AGAGGAAAGA CATCACTTGA TACTTCCAAT GATCTAGGGC CTTTACCTCC AGGATGGGAA 
GAGAGAACTC ACACAGATGG AAGAATCTTC TACATAAATC ACAATATAAA AAGAACACAA 
TGGGAAGATC CTCGGTTGGA GAATGTAGCA ATAACTGGAC CAGCAGTGCC CTACTCCAGG 
GATTACAAAA GAAAGTATGA GTTCTTCCGA AGAAAGTTGA AGAAGCAGAA TGACATTCCA 
AACAAATTTG AAATGAAACT TCGCCGAGCA ACTGTTCTTG AAGACTCTTA CCGGAGAATT 
ATGGGTGTCA AGAGAGCAGA CTTCCTGAAG GCTCGACTGT GGATTGAGTT TGATGGTGAA 
AAGGGATTGG ATTATGGAGG AGTTGCCAGA GAATGGTTCT TCCTGATCTC AAAGGAAATG 
TTTAACCCTT ATTATGGGTT GTTTGAATAT TCTGCTACGG ACAATTATAC CCTACAGATA 
AATCCAAACT CTGGATTGTG TAACGAAGAT CACCTCTCTT ACTTCAAGTT TATTGGTCGG 
GTAGCTGGAA TGGCAGTTTA TCATGGCAAA CTGTTGGATG GTTTTTTCAT CCGCCCATTT 
TACAAGATGA TGCTTCACAA ACCAATAACC CTTCATGATA TGGAATCTGT GGATAGTGAA 
TATTACAATT CCCTAAGATG GATTCTTGAA AATGACCCAA CAGAATTGGA CCTCAGGTTT 
ATCATAGATG AAGAACTTTT TGGACAGACA CATCAACATG AGCTGAAAAA TGGTGGATCA 
GAAATAGTTG TCACCAATAA GAACAAAAAG GAATATATTT ATCTTGTAAT ACAATGGCGA 
TTTGTAAACC GAATCCAGAA GCAAATGGCT GCTTTTAAAG AGGGATTCTT TGAACTAATA 
CCACAGGATC TCATCAAAAT TTTTGATGAA AATGAACTAG AGCTTCTTAT GTGTGGACTG 
GGAGATGTTG ATGTGAATGA CTGGAGGGAA CATACAAAGT ATAAAAATGG CTACAGTGCA 
AATCATCAGG TTATACAGTG GTTTTGGAAG GCTGTTTTAA TGATGGATTC AGAAAAAAGA 
ATAAGATTAC TTCAGTTTGT CACTGGCACA TCTCGGGTGC CTATGAATGG ATTTGCTGAA 
CTATACGGTT CAAATGGACC ACAGTCATTT ACAGTTGAAC AGTGGGGTAC TCCTGAAAAG 
CTGCCAAGAG CTCATACCTG TTTTAATCGC CTGGACTTGC CACCTTATGA ATCATTTGAA 
GAATTATGGG ATAAACTTCA GATGGCAATT GAAAACACCC AGGGCTTTGA TGGAGTTGAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006145.2
CDS	58..2760
Translation	MATCAVEVFGLLEDEENSRIVRVRVIAGIGLAKKDILGASDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	NM_006154.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006154.4

ORF Insert Sequence:

ATGGCAACTT GCGCGGTGGA GGTGTTCGGG CTCCTGGAGG ACGAGGAAAA TTCACGAATT 
GTGAGAGTAA GAGTTATAGC CGGAATAGGC CTTGCCAAGA AGGATATATT GGGAGCTAGT 
GATCCTTACG TGAGAGTGAC GTTATATGAC CCAATGAATG GAGTTCTTAC AAGTGTGCAA 
ACAAAAACCA TTAAAAAGAG TTTGAATCCA AAGTGGAATG AAGAAATATT ATTCAGAGTT 
CATCCTCAGC AGCACCGGCT TCTTTTTGAA GTGTTTGACG AAAACCGATT GACAAGAGAT 
GATTTCCTAG GTCAAGTGGA TGTTCCACTT TATCCATTAC CGACAGAAAA TCCAAGATTG 
GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
AAAGGTTATC TGAGACTAAA AATGACTTAT TTACCTAAAA CCAGTGGCTC AGAAGATGAT 
AATGCAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTTG TTTTGGACCA ACCAGATGCT 
GCTTGCCATT TGCAGCAACA ACAAGAACCT TCTCCTCTAC CTCCAGGGTG GGAAGAGAGG 
CAGGATATCC TTGGAAGGAC CTATTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
AGACCAACCC CTCAGGACAA CCTAACAGAT GCTGAGAATG GCAACATTCA ACTGCAAGCA 
CAACGTGCAT TTACCACCAG GCGGCAGATA TCCGAGGAAA CAGAAAGTGT TGACAACCGA 
GAGTCTTCCG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TAGCAACCAG 
GCCTTCCCAT CACCTCCACC GTCAAGTAAC TTGGATGTTC CAACTCATCT TGCAGAAGAA 
TTGAATGCCA GACTCACCAT TTTTGGAAAT TCAGCCGTGA GCCAGCCAGC ATCGAGCTCA 
AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA ACCTACACTT 
CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
AGAGGAAGAT CATATTATGT AGATCACAAT TCCAGAACGA CTACTTGGAC AAAGCCCACT 
GTACAGGCCA CAGTGGAGAC CAGTCAGCTG ACCTCAAGCC AGAGTTCTGC AGGCCCTCAA 
TCACAAGCCT CCACCAGTGA TTCAGGCCAG CAGGTGACCC AGCCATCTGA AATTGAGCAA 
GGATTCCTTC CTAAAGGCTG GGAAGTCCGG CATGCACCAA ATGGGAGGCC TTTCTTTATT 
GACCACAACA CTAAAACCAC CACCTGGGAA GATCCAAGAT TGAAAATTCC AGCCCATCTG 
AGAGGAAAGA CATCACTTGA TACTTCCAAT GATCTAGGGC CTTTACCTCC AGGATGGGAA 
GAGAGAACTC ACACAGATGG AAGAATCTTC TACATAAATC ACAATATAAA AAGAACACAA 
TGGGAAGATC CTCGGTTGGA GAATGTAGCA ATAACTGGAC CAGCAGTGCC CTACTCCAGG 
GATTACAAAA GAAAGTATGA GTTCTTCCGA AGAAAGTTGA AGAAGCAGAA TGACATTCCA 
AACAAATTTG AAATGAAACT TCGCCGAGCA ACTGTTCTTG AAGACTCTTA CCGGAGAATT 
ATGGGTGTCA AGAGAGCAGA CTTCCTGAAG GCTCGACTGT GGATTGAGTT TGATGGTGAA 
AAGGGATTGG ATTATGGAGG AGTTGCCAGA GAATGGTTCT TCCTGATCTC AAAGGAAATG 
TTTAACCCTT ATTATGGGTT GTTTGAATAT TCTGCTACGG ACAATTATAC CCTACAGATA 
AATCCAAACT CTGGATTGTG TAACGAAGAT CACCTCTCTT ACTTCAAGTT TATTGGTCGG 
GTAGCTGGAA TGGCAGTTTA TCATGGCAAA CTGTTGGATG GTTTTTTCAT CCGCCCATTT 
TACAAGATGA TGCTTCACAA ACCAATAACC CTTCATGATA TGGAATCTGT GGATAGTGAA 
TATTACAATT CCCTAAGATG GATTCTTGAA AATGACCCAA CAGAATTGGA CCTCAGGTTT 
ATCATAGATG AAGAACTTTT TGGACAGACA CATCAACATG AGCTGAAAAA TGGTGGATCA 
GAAATAGTTG TCACCAATAA GAACAAAAAG GAATATATTT ATCTTGTAAT ACAATGGCGA 
TTTGTAAACC GAATCCAGAA GCAAATGGCT GCTTTTAAAG AGGGATTCTT TGAACTAATA 
CCACAGGATC TCATCAAAAT TTTTGATGAA AATGAACTAG AGCTTCTTAT GTGTGGACTG 
GGAGATGTTG ATGTGAATGA CTGGAGGGAA CATACAAAGT ATAAAAATGG CTACAGTGCA 
AATCATCAGG TTATACAGTG GTTTTGGAAG GCTGTTTTAA TGATGGATTC AGAAAAAAGA 
ATAAGATTAC TTCAGTTTGT CACTGGCACA TCTCGGGTGC CTATGAATGG ATTTGCTGAA 
CTATACGGTT CAAATGGACC ACAGTCATTT ACAGTTGAAC AGTGGGGTAC TCCTGAAAAG 
CTGCCAAGAG CTCATACCTG TTTTAATCGC CTGGACTTGC CACCTTATGA ATCATTTGAA 
GAATTATGGG ATAAACTTCA GATGGCAATT GAAAACACCC AGGGCTTTGA TGGAGTTGAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( XM_011521624.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519926.1 Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

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CloneID	OHu59237
Clone ID Related Accession (Same CDS sequence)	XM_011521624.3 , XM_011521624.2
Accession Version	XM_011521624.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521624.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCGAG TGTTCTGGTG CCTTCGAGTT TGGCCAATTA AGAGTGATCC TTACGTGAGA 
GTGACGTTAT ATGACCCAAT GAATGGAGTT CTTACAAGTG TGCAAACAAA AACCATTAAA 
AAGAGTTTGA ATCCAAAGTG GAATGAAGAA ATATTATTCA GAGTTCATCC TCAGCAGCAC 
CGGCTTCTTT TTGAAGTGTT TGACGAAAAC CGATTGACAA GAGATGATTT CCTAGGTCAA 
GTGGATGTTC CACTTTATCC ATTACCGACA GAAAATCCAA GATTGGAGAG ACCATATACA 
TTTAAGGATT TTGTTCTTCA TCCAAGAAGT CACAAATCAA GAGTTAAAGG TTATCTGAGA 
CTAAAAATGA CTTATTTACC TAAAACCAGT GGCTCAGAAG ATGATAATGC AGAACAGGCT 
GAGGAATTAG AGCCTGGCTG GGTTGTTTTG GACCAACCAG ATGCTGCTTG CCATTTGCAG 
CAACAACAAG AACCTTCTCC TCTACCTCCA GGGTGGGAAG AGAGGCAGGA TATCCTTGGA 
AGGACCTATT ATGTAAACCA TGAATCTAGA AGAACACAGT GGAAAAGACC AACCCCTCAG 
GACAACCTAA CAGATGCTGA GAATGGCAAC ATTCAACTGC AAGCACAACG TGCATTTACC 
ACCAGGCGGC AGATATCCGA GGAAACAGAA AGTGTTGACA ACCGAGAGTC TTCCGAGAAC 
TGGGAAATTA TAAGAGAAGA TGAAGCCACC ATGTATAGCA ACCAGGCCTT CCCATCACCT 
CCACCGTCAA GTAACTTGGA TGTTCCAACT CATCTTGCAG AAGAATTGAA TGCCAGACTC 
ACCATTTTTG GAAATTCAGC CGTGAGCCAG CCAGCATCGA GCTCAAATCA TTCCAGCAGA 
AGAGGCAGCT TACAAGCCTA TACTTTTGAG GAACAACCTA CACTTCCTGT GCTTTTGCCT 
ACTTCATCTG GATTACCACC AGGTTGGGAA GAAAAACAAG ATGAAAGAGG AAGATCATAT 
TATGTAGATC ACAATTCCAG AACGACTACT TGGACAAAGC CCACTGTACA GGCCACAGTG 
GAGACCAGTC AGCTGACCTC AAGCCAGAGT TCTGCAGGCC CTCAATCACA AGCCTCCACC 
AGTGATTCAG GCCAGCAGGT GACCCAGCCA TCTGAAATTG AGCAAGGATT CCTTCCTAAA 
GGCTGGGAAG TCCGGCATGC ACCAAATGGG AGGCCTTTCT TTATTGACCA CAACACTAAA 
ACCACCACCT GGGAAGATCC AAGATTGAAA ATTCCAGCCC ATCTGAGAGG AAAGACATCA 
CTTGATACTT CCAATGATCT AGGGCCTTTA CCTCCAGGAT GGGAAGAGAG AACTCACACA 
GATGGAAGAA TCTTCTACAT AAATCACAAT ATAAAAAGAA CACAATGGGA AGATCCTCGG 
TTGGAGAATG TAGCAATAAC TGGACCAGCA GTGCCCTACT CCAGGGATTA CAAAAGAAAG 
TATGAGTTCT TCCGAAGAAA GTTGAAGAAG CAGAATGACA TTCCAAACAA ATTTGAAATG 
AAACTTCGCC GAGCAACTGT TCTTGAAGAC TCTTACCGGA GAATTATGGG TGTCAAGAGA 
GCAGACTTCC TGAAGGCTCG ACTGTGGATT GAGTTTGATG GTGAAAAGGG ATTGGATTAT 
GGAGGAGTTG CCAGAGAATG GTTCTTCCTG ATCTCAAAGG AAATGTTTAA CCCTTATTAT 
GGGTTGTTTG AATATTCTGC TACGGACAAT TATACCCTAC AGATAAATCC AAACTCTGGA 
TTGTGTAACG AAGATCACCT CTCTTACTTC AAGTTTATTG GTCGGGTAGC TGGAATGGCA 
GTTTATCATG GCAAACTGTT GGATGGTTTT TTCATCCGCC CATTTTACAA GATGATGCTT 
CACAAACCAA TAACCCTTCA TGATATGGAA TCTGTGGATA GTGAATATTA CAATTCCCTA 
AGATGGATTC TTGAAAATGA CCCAACAGAA TTGGACCTCA GGTTTATCAT AGATGAAGAA 
CTTTTTGGAC AGACACATCA ACATGAGCTG AAAAATGGTG GATCAGAAAT AGTTGTCACC 
AATAAGAACA AAAAGGAATA TATTTATCTT GTAATACAAT GGCGATTTGT AAACCGAATC 
CAGAAGCAAA TGGCTGCTTT TAAAGAGGGA TTCTTTGAAC TAATACCACA GGATCTCATC 
AAAATTTTTG ATGAAAATGA ACTAGAGCTT CTTATGTGTG GACTGGGAGA TGTTGATGTG 
AATGACTGGA GGGAACATAC AAAGTATAAA AATGGCTACA GTGCAAATCA TCAGGTTATA 
CAGTGGTTTT GGAAGGCTGT TTTAATGATG GATTCAGAAA AAAGAATAAG ATTACTTCAG 
TTTGTCACTG GCACATCTCG GGTGCCTATG AATGGATTTG CTGAACTATA CGGTTCAAAT 
GGACCACAGT CATTTACAGT TGAACAGTGG GGTACTCCTG AAAAGCTGCC AAGAGCTCAT 
ACCTGTTTTA ATCGCCTGGA CTTGCCACCT TATGAATCAT TTGAAGAATT ATGGGATAAA 
CTTCAGATGG CAATTGAAAA CACCCAGGGC TTTGATGGAG TTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519926.1
CDS	27..2654
Translation	MLRVFWCLRVWPIKSDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	XM_011521624.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521624.3

ORF Insert Sequence:

ATGTTGCGAG TGTTCTGGTG CCTTCGAGTT TGGCCAATTA AGAGTGATCC TTACGTGAGA 
GTGACGTTAT ATGACCCAAT GAATGGAGTT CTTACAAGTG TGCAAACAAA AACCATTAAA 
AAGAGTTTGA ATCCAAAGTG GAATGAAGAA ATATTATTCA GAGTTCATCC TCAGCAGCAC 
CGGCTTCTTT TTGAAGTGTT TGACGAAAAC CGATTGACAA GAGATGATTT CCTAGGTCAA 
GTGGATGTTC CACTTTATCC ATTACCGACA GAAAATCCAA GATTGGAGAG ACCATATACA 
TTTAAGGATT TTGTTCTTCA TCCAAGAAGT CACAAATCAA GAGTTAAAGG TTATCTGAGA 
CTAAAAATGA CTTATTTACC TAAAACCAGT GGCTCAGAAG ATGATAATGC AGAACAGGCT 
GAGGAATTAG AGCCTGGCTG GGTTGTTTTG GACCAACCAG ATGCTGCTTG CCATTTGCAG 
CAACAACAAG AACCTTCTCC TCTACCTCCA GGGTGGGAAG AGAGGCAGGA TATCCTTGGA 
AGGACCTATT ATGTAAACCA TGAATCTAGA AGAACACAGT GGAAAAGACC AACCCCTCAG 
GACAACCTAA CAGATGCTGA GAATGGCAAC ATTCAACTGC AAGCACAACG TGCATTTACC 
ACCAGGCGGC AGATATCCGA GGAAACAGAA AGTGTTGACA ACCGAGAGTC TTCCGAGAAC 
TGGGAAATTA TAAGAGAAGA TGAAGCCACC ATGTATAGCA ACCAGGCCTT CCCATCACCT 
CCACCGTCAA GTAACTTGGA TGTTCCAACT CATCTTGCAG AAGAATTGAA TGCCAGACTC 
ACCATTTTTG GAAATTCAGC CGTGAGCCAG CCAGCATCGA GCTCAAATCA TTCCAGCAGA 
AGAGGCAGCT TACAAGCCTA TACTTTTGAG GAACAACCTA CACTTCCTGT GCTTTTGCCT 
ACTTCATCTG GATTACCACC AGGTTGGGAA GAAAAACAAG ATGAAAGAGG AAGATCATAT 
TATGTAGATC ACAATTCCAG AACGACTACT TGGACAAAGC CCACTGTACA GGCCACAGTG 
GAGACCAGTC AGCTGACCTC AAGCCAGAGT TCTGCAGGCC CTCAATCACA AGCCTCCACC 
AGTGATTCAG GCCAGCAGGT GACCCAGCCA TCTGAAATTG AGCAAGGATT CCTTCCTAAA 
GGCTGGGAAG TCCGGCATGC ACCAAATGGG AGGCCTTTCT TTATTGACCA CAACACTAAA 
ACCACCACCT GGGAAGATCC AAGATTGAAA ATTCCAGCCC ATCTGAGAGG AAAGACATCA 
CTTGATACTT CCAATGATCT AGGGCCTTTA CCTCCAGGAT GGGAAGAGAG AACTCACACA 
GATGGAAGAA TCTTCTACAT AAATCACAAT ATAAAAAGAA CACAATGGGA AGATCCTCGG 
TTGGAGAATG TAGCAATAAC TGGACCAGCA GTGCCCTACT CCAGGGATTA CAAAAGAAAG 
TATGAGTTCT TCCGAAGAAA GTTGAAGAAG CAGAATGACA TTCCAAACAA ATTTGAAATG 
AAACTTCGCC GAGCAACTGT TCTTGAAGAC TCTTACCGGA GAATTATGGG TGTCAAGAGA 
GCAGACTTCC TGAAGGCTCG ACTGTGGATT GAGTTTGATG GTGAAAAGGG ATTGGATTAT 
GGAGGAGTTG CCAGAGAATG GTTCTTCCTG ATCTCAAAGG AAATGTTTAA CCCTTATTAT 
GGGTTGTTTG AATATTCTGC TACGGACAAT TATACCCTAC AGATAAATCC AAACTCTGGA 
TTGTGTAACG AAGATCACCT CTCTTACTTC AAGTTTATTG GTCGGGTAGC TGGAATGGCA 
GTTTATCATG GCAAACTGTT GGATGGTTTT TTCATCCGCC CATTTTACAA GATGATGCTT 
CACAAACCAA TAACCCTTCA TGATATGGAA TCTGTGGATA GTGAATATTA CAATTCCCTA 
AGATGGATTC TTGAAAATGA CCCAACAGAA TTGGACCTCA GGTTTATCAT AGATGAAGAA 
CTTTTTGGAC AGACACATCA ACATGAGCTG AAAAATGGTG GATCAGAAAT AGTTGTCACC 
AATAAGAACA AAAAGGAATA TATTTATCTT GTAATACAAT GGCGATTTGT AAACCGAATC 
CAGAAGCAAA TGGCTGCTTT TAAAGAGGGA TTCTTTGAAC TAATACCACA GGATCTCATC 
AAAATTTTTG ATGAAAATGA ACTAGAGCTT CTTATGTGTG GACTGGGAGA TGTTGATGTG 
AATGACTGGA GGGAACATAC AAAGTATAAA AATGGCTACA GTGCAAATCA TCAGGTTATA 
CAGTGGTTTT GGAAGGCTGT TTTAATGATG GATTCAGAAA AAAGAATAAG ATTACTTCAG 
TTTGTCACTG GCACATCTCG GGTGCCTATG AATGGATTTG CTGAACTATA CGGTTCAAAT 
GGACCACAGT CATTTACAGT TGAACAGTGG GGTACTCCTG AAAAGCTGCC AAGAGCTCAT 
ACCTGTTTTA ATCGCCTGGA CTTGCCACCT TATGAATCAT TTGAAGAATT ATGGGATAAA 
CTTCAGATGG CAATTGAAAA CACCCAGGGC TTTGATGGAG TTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( XM_011521624.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519926.1 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu59237
Clone ID Related Accession (Same CDS sequence)	XM_011521624.3 , XM_011521624.2
Accession Version	XM_011521624.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521624.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCGAG TGTTCTGGTG CCTTCGAGTT TGGCCAATTA AGAGTGATCC TTACGTGAGA 
GTGACGTTAT ATGACCCAAT GAATGGAGTT CTTACAAGTG TGCAAACAAA AACCATTAAA 
AAGAGTTTGA ATCCAAAGTG GAATGAAGAA ATATTATTCA GAGTTCATCC TCAGCAGCAC 
CGGCTTCTTT TTGAAGTGTT TGACGAAAAC CGATTGACAA GAGATGATTT CCTAGGTCAA 
GTGGATGTTC CACTTTATCC ATTACCGACA GAAAATCCAA GATTGGAGAG ACCATATACA 
TTTAAGGATT TTGTTCTTCA TCCAAGAAGT CACAAATCAA GAGTTAAAGG TTATCTGAGA 
CTAAAAATGA CTTATTTACC TAAAACCAGT GGCTCAGAAG ATGATAATGC AGAACAGGCT 
GAGGAATTAG AGCCTGGCTG GGTTGTTTTG GACCAACCAG ATGCTGCTTG CCATTTGCAG 
CAACAACAAG AACCTTCTCC TCTACCTCCA GGGTGGGAAG AGAGGCAGGA TATCCTTGGA 
AGGACCTATT ATGTAAACCA TGAATCTAGA AGAACACAGT GGAAAAGACC AACCCCTCAG 
GACAACCTAA CAGATGCTGA GAATGGCAAC ATTCAACTGC AAGCACAACG TGCATTTACC 
ACCAGGCGGC AGATATCCGA GGAAACAGAA AGTGTTGACA ACCGAGAGTC TTCCGAGAAC 
TGGGAAATTA TAAGAGAAGA TGAAGCCACC ATGTATAGCA ACCAGGCCTT CCCATCACCT 
CCACCGTCAA GTAACTTGGA TGTTCCAACT CATCTTGCAG AAGAATTGAA TGCCAGACTC 
ACCATTTTTG GAAATTCAGC CGTGAGCCAG CCAGCATCGA GCTCAAATCA TTCCAGCAGA 
AGAGGCAGCT TACAAGCCTA TACTTTTGAG GAACAACCTA CACTTCCTGT GCTTTTGCCT 
ACTTCATCTG GATTACCACC AGGTTGGGAA GAAAAACAAG ATGAAAGAGG AAGATCATAT 
TATGTAGATC ACAATTCCAG AACGACTACT TGGACAAAGC CCACTGTACA GGCCACAGTG 
GAGACCAGTC AGCTGACCTC AAGCCAGAGT TCTGCAGGCC CTCAATCACA AGCCTCCACC 
AGTGATTCAG GCCAGCAGGT GACCCAGCCA TCTGAAATTG AGCAAGGATT CCTTCCTAAA 
GGCTGGGAAG TCCGGCATGC ACCAAATGGG AGGCCTTTCT TTATTGACCA CAACACTAAA 
ACCACCACCT GGGAAGATCC AAGATTGAAA ATTCCAGCCC ATCTGAGAGG AAAGACATCA 
CTTGATACTT CCAATGATCT AGGGCCTTTA CCTCCAGGAT GGGAAGAGAG AACTCACACA 
GATGGAAGAA TCTTCTACAT AAATCACAAT ATAAAAAGAA CACAATGGGA AGATCCTCGG 
TTGGAGAATG TAGCAATAAC TGGACCAGCA GTGCCCTACT CCAGGGATTA CAAAAGAAAG 
TATGAGTTCT TCCGAAGAAA GTTGAAGAAG CAGAATGACA TTCCAAACAA ATTTGAAATG 
AAACTTCGCC GAGCAACTGT TCTTGAAGAC TCTTACCGGA GAATTATGGG TGTCAAGAGA 
GCAGACTTCC TGAAGGCTCG ACTGTGGATT GAGTTTGATG GTGAAAAGGG ATTGGATTAT 
GGAGGAGTTG CCAGAGAATG GTTCTTCCTG ATCTCAAAGG AAATGTTTAA CCCTTATTAT 
GGGTTGTTTG AATATTCTGC TACGGACAAT TATACCCTAC AGATAAATCC AAACTCTGGA 
TTGTGTAACG AAGATCACCT CTCTTACTTC AAGTTTATTG GTCGGGTAGC TGGAATGGCA 
GTTTATCATG GCAAACTGTT GGATGGTTTT TTCATCCGCC CATTTTACAA GATGATGCTT 
CACAAACCAA TAACCCTTCA TGATATGGAA TCTGTGGATA GTGAATATTA CAATTCCCTA 
AGATGGATTC TTGAAAATGA CCCAACAGAA TTGGACCTCA GGTTTATCAT AGATGAAGAA 
CTTTTTGGAC AGACACATCA ACATGAGCTG AAAAATGGTG GATCAGAAAT AGTTGTCACC 
AATAAGAACA AAAAGGAATA TATTTATCTT GTAATACAAT GGCGATTTGT AAACCGAATC 
CAGAAGCAAA TGGCTGCTTT TAAAGAGGGA TTCTTTGAAC TAATACCACA GGATCTCATC 
AAAATTTTTG ATGAAAATGA ACTAGAGCTT CTTATGTGTG GACTGGGAGA TGTTGATGTG 
AATGACTGGA GGGAACATAC AAAGTATAAA AATGGCTACA GTGCAAATCA TCAGGTTATA 
CAGTGGTTTT GGAAGGCTGT TTTAATGATG GATTCAGAAA AAAGAATAAG ATTACTTCAG 
TTTGTCACTG GCACATCTCG GGTGCCTATG AATGGATTTG CTGAACTATA CGGTTCAAAT 
GGACCACAGT CATTTACAGT TGAACAGTGG GGTACTCCTG AAAAGCTGCC AAGAGCTCAT 
ACCTGTTTTA ATCGCCTGGA CTTGCCACCT TATGAATCAT TTGAAGAATT ATGGGATAAA 
CTTCAGATGG CAATTGAAAA CACCCAGGGC TTTGATGGAG TTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519926.1
CDS	32..2659
Translation	MLRVFWCLRVWPIKSDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	XM_011521624.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521624.2

ORF Insert Sequence:

ATGTTGCGAG TGTTCTGGTG CCTTCGAGTT TGGCCAATTA AGAGTGATCC TTACGTGAGA 
GTGACGTTAT ATGACCCAAT GAATGGAGTT CTTACAAGTG TGCAAACAAA AACCATTAAA 
AAGAGTTTGA ATCCAAAGTG GAATGAAGAA ATATTATTCA GAGTTCATCC TCAGCAGCAC 
CGGCTTCTTT TTGAAGTGTT TGACGAAAAC CGATTGACAA GAGATGATTT CCTAGGTCAA 
GTGGATGTTC CACTTTATCC ATTACCGACA GAAAATCCAA GATTGGAGAG ACCATATACA 
TTTAAGGATT TTGTTCTTCA TCCAAGAAGT CACAAATCAA GAGTTAAAGG TTATCTGAGA 
CTAAAAATGA CTTATTTACC TAAAACCAGT GGCTCAGAAG ATGATAATGC AGAACAGGCT 
GAGGAATTAG AGCCTGGCTG GGTTGTTTTG GACCAACCAG ATGCTGCTTG CCATTTGCAG 
CAACAACAAG AACCTTCTCC TCTACCTCCA GGGTGGGAAG AGAGGCAGGA TATCCTTGGA 
AGGACCTATT ATGTAAACCA TGAATCTAGA AGAACACAGT GGAAAAGACC AACCCCTCAG 
GACAACCTAA CAGATGCTGA GAATGGCAAC ATTCAACTGC AAGCACAACG TGCATTTACC 
ACCAGGCGGC AGATATCCGA GGAAACAGAA AGTGTTGACA ACCGAGAGTC TTCCGAGAAC 
TGGGAAATTA TAAGAGAAGA TGAAGCCACC ATGTATAGCA ACCAGGCCTT CCCATCACCT 
CCACCGTCAA GTAACTTGGA TGTTCCAACT CATCTTGCAG AAGAATTGAA TGCCAGACTC 
ACCATTTTTG GAAATTCAGC CGTGAGCCAG CCAGCATCGA GCTCAAATCA TTCCAGCAGA 
AGAGGCAGCT TACAAGCCTA TACTTTTGAG GAACAACCTA CACTTCCTGT GCTTTTGCCT 
ACTTCATCTG GATTACCACC AGGTTGGGAA GAAAAACAAG ATGAAAGAGG AAGATCATAT 
TATGTAGATC ACAATTCCAG AACGACTACT TGGACAAAGC CCACTGTACA GGCCACAGTG 
GAGACCAGTC AGCTGACCTC AAGCCAGAGT TCTGCAGGCC CTCAATCACA AGCCTCCACC 
AGTGATTCAG GCCAGCAGGT GACCCAGCCA TCTGAAATTG AGCAAGGATT CCTTCCTAAA 
GGCTGGGAAG TCCGGCATGC ACCAAATGGG AGGCCTTTCT TTATTGACCA CAACACTAAA 
ACCACCACCT GGGAAGATCC AAGATTGAAA ATTCCAGCCC ATCTGAGAGG AAAGACATCA 
CTTGATACTT CCAATGATCT AGGGCCTTTA CCTCCAGGAT GGGAAGAGAG AACTCACACA 
GATGGAAGAA TCTTCTACAT AAATCACAAT ATAAAAAGAA CACAATGGGA AGATCCTCGG 
TTGGAGAATG TAGCAATAAC TGGACCAGCA GTGCCCTACT CCAGGGATTA CAAAAGAAAG 
TATGAGTTCT TCCGAAGAAA GTTGAAGAAG CAGAATGACA TTCCAAACAA ATTTGAAATG 
AAACTTCGCC GAGCAACTGT TCTTGAAGAC TCTTACCGGA GAATTATGGG TGTCAAGAGA 
GCAGACTTCC TGAAGGCTCG ACTGTGGATT GAGTTTGATG GTGAAAAGGG ATTGGATTAT 
GGAGGAGTTG CCAGAGAATG GTTCTTCCTG ATCTCAAAGG AAATGTTTAA CCCTTATTAT 
GGGTTGTTTG AATATTCTGC TACGGACAAT TATACCCTAC AGATAAATCC AAACTCTGGA 
TTGTGTAACG AAGATCACCT CTCTTACTTC AAGTTTATTG GTCGGGTAGC TGGAATGGCA 
GTTTATCATG GCAAACTGTT GGATGGTTTT TTCATCCGCC CATTTTACAA GATGATGCTT 
CACAAACCAA TAACCCTTCA TGATATGGAA TCTGTGGATA GTGAATATTA CAATTCCCTA 
AGATGGATTC TTGAAAATGA CCCAACAGAA TTGGACCTCA GGTTTATCAT AGATGAAGAA 
CTTTTTGGAC AGACACATCA ACATGAGCTG AAAAATGGTG GATCAGAAAT AGTTGTCACC 
AATAAGAACA AAAAGGAATA TATTTATCTT GTAATACAAT GGCGATTTGT AAACCGAATC 
CAGAAGCAAA TGGCTGCTTT TAAAGAGGGA TTCTTTGAAC TAATACCACA GGATCTCATC 
AAAATTTTTG ATGAAAATGA ACTAGAGCTT CTTATGTGTG GACTGGGAGA TGTTGATGTG 
AATGACTGGA GGGAACATAC AAAGTATAAA AATGGCTACA GTGCAAATCA TCAGGTTATA 
CAGTGGTTTT GGAAGGCTGT TTTAATGATG GATTCAGAAA AAAGAATAAG ATTACTTCAG 
TTTGTCACTG GCACATCTCG GGTGCCTATG AATGGATTTG CTGAACTATA CGGTTCAAAT 
GGACCACAGT CATTTACAGT TGAACAGTGG GGTACTCCTG AAAAGCTGCC AAGAGCTCAT 
ACCTGTTTTA ATCGCCTGGA CTTGCCACCT TATGAATCAT TTGAAGAATT ATGGGATAAA 
CTTCAGATGG CAATTGAAAA CACCCAGGGC TTTGATGGAG TTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( XM_011521625.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519927.1 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu59238
Clone ID Related Accession (Same CDS sequence)	XM_011521625.2 , XM_011521626.1 , XM_011521625.3
Accession Version	XM_011521625.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521625.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG TTCTTACAAG TGTGCAAACA AAAACCATTA AAAAGAGTTT GAATCCAAAG 
TGGAATGAAG AAATATTATT CAGAGTTCAT CCTCAGCAGC ACCGGCTTCT TTTTGAAGTG 
TTTGACGAAA ACCGATTGAC AAGAGATGAT TTCCTAGGTC AAGTGGATGT TCCACTTTAT 
CCATTACCGA CAGAAAATCC AAGATTGGAG AGACCATATA CATTTAAGGA TTTTGTTCTT 
CATCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATCTGA GACTAAAAAT GACTTATTTA 
CCTAAAACCA GTGGCTCAGA AGATGATAAT GCAGAACAGG CTGAGGAATT AGAGCCTGGC 
TGGGTTGTTT TGGACCAACC AGATGCTGCT TGCCATTTGC AGCAACAACA AGAACCTTCT 
CCTCTACCTC CAGGGTGGGA AGAGAGGCAG GATATCCTTG GAAGGACCTA TTATGTAAAC 
CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCCCTC AGGACAACCT AACAGATGCT 
GAGAATGGCA ACATTCAACT GCAAGCACAA CGTGCATTTA CCACCAGGCG GCAGATATCC 
GAGGAAACAG AAAGTGTTGA CAACCGAGAG TCTTCCGAGA ACTGGGAAAT TATAAGAGAA 
GATGAAGCCA CCATGTATAG CAACCAGGCC TTCCCATCAC CTCCACCGTC AAGTAACTTG 
GATGTTCCAA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACCATTTT TGGAAATTCA 
GCCGTGAGCC AGCCAGCATC GAGCTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC 
TATACTTTTG AGGAACAACC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA 
CCAGGTTGGG AAGAAAAACA AGATGAAAGA GGAAGATCAT ATTATGTAGA TCACAATTCC 
AGAACGACTA CTTGGACAAA GCCCACTGTA CAGGCCACAG TGGAGACCAG TCAGCTGACC 
TCAAGCCAGA GTTCTGCAGG CCCTCAATCA CAAGCCTCCA CCAGTGATTC AGGCCAGCAG 
GTGACCCAGC CATCTGAAAT TGAGCAAGGA TTCCTTCCTA AAGGCTGGGA AGTCCGGCAT 
GCACCAAATG GGAGGCCTTT CTTTATTGAC CACAACACTA AAACCACCAC CTGGGAAGAT 
CCAAGATTGA AAATTCCAGC CCATCTGAGA GGAAAGACAT CACTTGATAC TTCCAATGAT 
CTAGGGCCTT TACCTCCAGG ATGGGAAGAG AGAACTCACA CAGATGGAAG AATCTTCTAC 
ATAAATCACA ATATAAAAAG AACACAATGG GAAGATCCTC GGTTGGAGAA TGTAGCAATA 
ACTGGACCAG CAGTGCCCTA CTCCAGGGAT TACAAAAGAA AGTATGAGTT CTTCCGAAGA 
AAGTTGAAGA AGCAGAATGA CATTCCAAAC AAATTTGAAA TGAAACTTCG CCGAGCAACT 
GTTCTTGAAG ACTCTTACCG GAGAATTATG GGTGTCAAGA GAGCAGACTT CCTGAAGGCT 
CGACTGTGGA TTGAGTTTGA TGGTGAAAAG GGATTGGATT ATGGAGGAGT TGCCAGAGAA 
TGGTTCTTCC TGATCTCAAA GGAAATGTTT AACCCTTATT ATGGGTTGTT TGAATATTCT 
GCTACGGACA ATTATACCCT ACAGATAAAT CCAAACTCTG GATTGTGTAA CGAAGATCAC 
CTCTCTTACT TCAAGTTTAT TGGTCGGGTA GCTGGAATGG CAGTTTATCA TGGCAAACTG 
TTGGATGGTT TTTTCATCCG CCCATTTTAC AAGATGATGC TTCACAAACC AATAACCCTT 
CATGATATGG AATCTGTGGA TAGTGAATAT TACAATTCCC TAAGATGGAT TCTTGAAAAT 
GACCCAACAG AATTGGACCT CAGGTTTATC ATAGATGAAG AACTTTTTGG ACAGACACAT 
CAACATGAGC TGAAAAATGG TGGATCAGAA ATAGTTGTCA CCAATAAGAA CAAAAAGGAA 
TATATTTATC TTGTAATACA ATGGCGATTT GTAAACCGAA TCCAGAAGCA AATGGCTGCT 
TTTAAAGAGG GATTCTTTGA ACTAATACCA CAGGATCTCA TCAAAATTTT TGATGAAAAT 
GAACTAGAGC TTCTTATGTG TGGACTGGGA GATGTTGATG TGAATGACTG GAGGGAACAT 
ACAAAGTATA AAAATGGCTA CAGTGCAAAT CATCAGGTTA TACAGTGGTT TTGGAAGGCT 
GTTTTAATGA TGGATTCAGA AAAAAGAATA AGATTACTTC AGTTTGTCAC TGGCACATCT 
CGGGTGCCTA TGAATGGATT TGCTGAACTA TACGGTTCAA ATGGACCACA GTCATTTACA 
GTTGAACAGT GGGGTACTCC TGAAAAGCTG CCAAGAGCTC ATACCTGTTT TAATCGCCTG 
GACTTGCCAC CTTATGAATC ATTTGAAGAA TTATGGGATA AACTTCAGAT GGCAATTGAA 
AACACCCAGG GCTTTGATGG AGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519927.1
CDS	283..2832
Translation	MNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	XM_011521625.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521625.2

ORF Insert Sequence:

ATGAATGGAG TTCTTACAAG TGTGCAAACA AAAACCATTA AAAAGAGTTT GAATCCAAAG 
TGGAATGAAG AAATATTATT CAGAGTTCAT CCTCAGCAGC ACCGGCTTCT TTTTGAAGTG 
TTTGACGAAA ACCGATTGAC AAGAGATGAT TTCCTAGGTC AAGTGGATGT TCCACTTTAT 
CCATTACCGA CAGAAAATCC AAGATTGGAG AGACCATATA CATTTAAGGA TTTTGTTCTT 
CATCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATCTGA GACTAAAAAT GACTTATTTA 
CCTAAAACCA GTGGCTCAGA AGATGATAAT GCAGAACAGG CTGAGGAATT AGAGCCTGGC 
TGGGTTGTTT TGGACCAACC AGATGCTGCT TGCCATTTGC AGCAACAACA AGAACCTTCT 
CCTCTACCTC CAGGGTGGGA AGAGAGGCAG GATATCCTTG GAAGGACCTA TTATGTAAAC 
CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCCCTC AGGACAACCT AACAGATGCT 
GAGAATGGCA ACATTCAACT GCAAGCACAA CGTGCATTTA CCACCAGGCG GCAGATATCC 
GAGGAAACAG AAAGTGTTGA CAACCGAGAG TCTTCCGAGA ACTGGGAAAT TATAAGAGAA 
GATGAAGCCA CCATGTATAG CAACCAGGCC TTCCCATCAC CTCCACCGTC AAGTAACTTG 
GATGTTCCAA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACCATTTT TGGAAATTCA 
GCCGTGAGCC AGCCAGCATC GAGCTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC 
TATACTTTTG AGGAACAACC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA 
CCAGGTTGGG AAGAAAAACA AGATGAAAGA GGAAGATCAT ATTATGTAGA TCACAATTCC 
AGAACGACTA CTTGGACAAA GCCCACTGTA CAGGCCACAG TGGAGACCAG TCAGCTGACC 
TCAAGCCAGA GTTCTGCAGG CCCTCAATCA CAAGCCTCCA CCAGTGATTC AGGCCAGCAG 
GTGACCCAGC CATCTGAAAT TGAGCAAGGA TTCCTTCCTA AAGGCTGGGA AGTCCGGCAT 
GCACCAAATG GGAGGCCTTT CTTTATTGAC CACAACACTA AAACCACCAC CTGGGAAGAT 
CCAAGATTGA AAATTCCAGC CCATCTGAGA GGAAAGACAT CACTTGATAC TTCCAATGAT 
CTAGGGCCTT TACCTCCAGG ATGGGAAGAG AGAACTCACA CAGATGGAAG AATCTTCTAC 
ATAAATCACA ATATAAAAAG AACACAATGG GAAGATCCTC GGTTGGAGAA TGTAGCAATA 
ACTGGACCAG CAGTGCCCTA CTCCAGGGAT TACAAAAGAA AGTATGAGTT CTTCCGAAGA 
AAGTTGAAGA AGCAGAATGA CATTCCAAAC AAATTTGAAA TGAAACTTCG CCGAGCAACT 
GTTCTTGAAG ACTCTTACCG GAGAATTATG GGTGTCAAGA GAGCAGACTT CCTGAAGGCT 
CGACTGTGGA TTGAGTTTGA TGGTGAAAAG GGATTGGATT ATGGAGGAGT TGCCAGAGAA 
TGGTTCTTCC TGATCTCAAA GGAAATGTTT AACCCTTATT ATGGGTTGTT TGAATATTCT 
GCTACGGACA ATTATACCCT ACAGATAAAT CCAAACTCTG GATTGTGTAA CGAAGATCAC 
CTCTCTTACT TCAAGTTTAT TGGTCGGGTA GCTGGAATGG CAGTTTATCA TGGCAAACTG 
TTGGATGGTT TTTTCATCCG CCCATTTTAC AAGATGATGC TTCACAAACC AATAACCCTT 
CATGATATGG AATCTGTGGA TAGTGAATAT TACAATTCCC TAAGATGGAT TCTTGAAAAT 
GACCCAACAG AATTGGACCT CAGGTTTATC ATAGATGAAG AACTTTTTGG ACAGACACAT 
CAACATGAGC TGAAAAATGG TGGATCAGAA ATAGTTGTCA CCAATAAGAA CAAAAAGGAA 
TATATTTATC TTGTAATACA ATGGCGATTT GTAAACCGAA TCCAGAAGCA AATGGCTGCT 
TTTAAAGAGG GATTCTTTGA ACTAATACCA CAGGATCTCA TCAAAATTTT TGATGAAAAT 
GAACTAGAGC TTCTTATGTG TGGACTGGGA GATGTTGATG TGAATGACTG GAGGGAACAT 
ACAAAGTATA AAAATGGCTA CAGTGCAAAT CATCAGGTTA TACAGTGGTT TTGGAAGGCT 
GTTTTAATGA TGGATTCAGA AAAAAGAATA AGATTACTTC AGTTTGTCAC TGGCACATCT 
CGGGTGCCTA TGAATGGATT TGCTGAACTA TACGGTTCAA ATGGACCACA GTCATTTACA 
GTTGAACAGT GGGGTACTCC TGAAAAGCTG CCAAGAGCTC ATACCTGTTT TAATCGCCTG 
GACTTGCCAC CTTATGAATC ATTTGAAGAA TTATGGGATA AACTTCAGAT GGCAATTGAA 
AACACCCAGG GCTTTGATGG AGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( XM_011521626.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519928.1 Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA.

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CloneID	OHu59238
Clone ID Related Accession (Same CDS sequence)	XM_011521625.2 , XM_011521626.1 , XM_011521625.3
Accession Version	XM_011521626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG TTCTTACAAG TGTGCAAACA AAAACCATTA AAAAGAGTTT GAATCCAAAG 
TGGAATGAAG AAATATTATT CAGAGTTCAT CCTCAGCAGC ACCGGCTTCT TTTTGAAGTG 
TTTGACGAAA ACCGATTGAC AAGAGATGAT TTCCTAGGTC AAGTGGATGT TCCACTTTAT 
CCATTACCGA CAGAAAATCC AAGATTGGAG AGACCATATA CATTTAAGGA TTTTGTTCTT 
CATCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATCTGA GACTAAAAAT GACTTATTTA 
CCTAAAACCA GTGGCTCAGA AGATGATAAT GCAGAACAGG CTGAGGAATT AGAGCCTGGC 
TGGGTTGTTT TGGACCAACC AGATGCTGCT TGCCATTTGC AGCAACAACA AGAACCTTCT 
CCTCTACCTC CAGGGTGGGA AGAGAGGCAG GATATCCTTG GAAGGACCTA TTATGTAAAC 
CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCCCTC AGGACAACCT AACAGATGCT 
GAGAATGGCA ACATTCAACT GCAAGCACAA CGTGCATTTA CCACCAGGCG GCAGATATCC 
GAGGAAACAG AAAGTGTTGA CAACCGAGAG TCTTCCGAGA ACTGGGAAAT TATAAGAGAA 
GATGAAGCCA CCATGTATAG CAACCAGGCC TTCCCATCAC CTCCACCGTC AAGTAACTTG 
GATGTTCCAA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACCATTTT TGGAAATTCA 
GCCGTGAGCC AGCCAGCATC GAGCTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC 
TATACTTTTG AGGAACAACC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA 
CCAGGTTGGG AAGAAAAACA AGATGAAAGA GGAAGATCAT ATTATGTAGA TCACAATTCC 
AGAACGACTA CTTGGACAAA GCCCACTGTA CAGGCCACAG TGGAGACCAG TCAGCTGACC 
TCAAGCCAGA GTTCTGCAGG CCCTCAATCA CAAGCCTCCA CCAGTGATTC AGGCCAGCAG 
GTGACCCAGC CATCTGAAAT TGAGCAAGGA TTCCTTCCTA AAGGCTGGGA AGTCCGGCAT 
GCACCAAATG GGAGGCCTTT CTTTATTGAC CACAACACTA AAACCACCAC CTGGGAAGAT 
CCAAGATTGA AAATTCCAGC CCATCTGAGA GGAAAGACAT CACTTGATAC TTCCAATGAT 
CTAGGGCCTT TACCTCCAGG ATGGGAAGAG AGAACTCACA CAGATGGAAG AATCTTCTAC 
ATAAATCACA ATATAAAAAG AACACAATGG GAAGATCCTC GGTTGGAGAA TGTAGCAATA 
ACTGGACCAG CAGTGCCCTA CTCCAGGGAT TACAAAAGAA AGTATGAGTT CTTCCGAAGA 
AAGTTGAAGA AGCAGAATGA CATTCCAAAC AAATTTGAAA TGAAACTTCG CCGAGCAACT 
GTTCTTGAAG ACTCTTACCG GAGAATTATG GGTGTCAAGA GAGCAGACTT CCTGAAGGCT 
CGACTGTGGA TTGAGTTTGA TGGTGAAAAG GGATTGGATT ATGGAGGAGT TGCCAGAGAA 
TGGTTCTTCC TGATCTCAAA GGAAATGTTT AACCCTTATT ATGGGTTGTT TGAATATTCT 
GCTACGGACA ATTATACCCT ACAGATAAAT CCAAACTCTG GATTGTGTAA CGAAGATCAC 
CTCTCTTACT TCAAGTTTAT TGGTCGGGTA GCTGGAATGG CAGTTTATCA TGGCAAACTG 
TTGGATGGTT TTTTCATCCG CCCATTTTAC AAGATGATGC TTCACAAACC AATAACCCTT 
CATGATATGG AATCTGTGGA TAGTGAATAT TACAATTCCC TAAGATGGAT TCTTGAAAAT 
GACCCAACAG AATTGGACCT CAGGTTTATC ATAGATGAAG AACTTTTTGG ACAGACACAT 
CAACATGAGC TGAAAAATGG TGGATCAGAA ATAGTTGTCA CCAATAAGAA CAAAAAGGAA 
TATATTTATC TTGTAATACA ATGGCGATTT GTAAACCGAA TCCAGAAGCA AATGGCTGCT 
TTTAAAGAGG GATTCTTTGA ACTAATACCA CAGGATCTCA TCAAAATTTT TGATGAAAAT 
GAACTAGAGC TTCTTATGTG TGGACTGGGA GATGTTGATG TGAATGACTG GAGGGAACAT 
ACAAAGTATA AAAATGGCTA CAGTGCAAAT CATCAGGTTA TACAGTGGTT TTGGAAGGCT 
GTTTTAATGA TGGATTCAGA AAAAAGAATA AGATTACTTC AGTTTGTCAC TGGCACATCT 
CGGGTGCCTA TGAATGGATT TGCTGAACTA TACGGTTCAA ATGGACCACA GTCATTTACA 
GTTGAACAGT GGGGTACTCC TGAAAAGCTG CCAAGAGCTC ATACCTGTTT TAATCGCCTG 
GACTTGCCAC CTTATGAATC ATTTGAAGAA TTATGGGATA AACTTCAGAT GGCAATTGAA 
AACACCCAGG GCTTTGATGG AGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519928.1
CDS	127..2676
Translation	MNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	XM_011521626.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521626.1

ORF Insert Sequence:

ATGAATGGAG TTCTTACAAG TGTGCAAACA AAAACCATTA AAAAGAGTTT GAATCCAAAG 
TGGAATGAAG AAATATTATT CAGAGTTCAT CCTCAGCAGC ACCGGCTTCT TTTTGAAGTG 
TTTGACGAAA ACCGATTGAC AAGAGATGAT TTCCTAGGTC AAGTGGATGT TCCACTTTAT 
CCATTACCGA CAGAAAATCC AAGATTGGAG AGACCATATA CATTTAAGGA TTTTGTTCTT 
CATCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATCTGA GACTAAAAAT GACTTATTTA 
CCTAAAACCA GTGGCTCAGA AGATGATAAT GCAGAACAGG CTGAGGAATT AGAGCCTGGC 
TGGGTTGTTT TGGACCAACC AGATGCTGCT TGCCATTTGC AGCAACAACA AGAACCTTCT 
CCTCTACCTC CAGGGTGGGA AGAGAGGCAG GATATCCTTG GAAGGACCTA TTATGTAAAC 
CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCCCTC AGGACAACCT AACAGATGCT 
GAGAATGGCA ACATTCAACT GCAAGCACAA CGTGCATTTA CCACCAGGCG GCAGATATCC 
GAGGAAACAG AAAGTGTTGA CAACCGAGAG TCTTCCGAGA ACTGGGAAAT TATAAGAGAA 
GATGAAGCCA CCATGTATAG CAACCAGGCC TTCCCATCAC CTCCACCGTC AAGTAACTTG 
GATGTTCCAA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACCATTTT TGGAAATTCA 
GCCGTGAGCC AGCCAGCATC GAGCTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC 
TATACTTTTG AGGAACAACC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA 
CCAGGTTGGG AAGAAAAACA AGATGAAAGA GGAAGATCAT ATTATGTAGA TCACAATTCC 
AGAACGACTA CTTGGACAAA GCCCACTGTA CAGGCCACAG TGGAGACCAG TCAGCTGACC 
TCAAGCCAGA GTTCTGCAGG CCCTCAATCA CAAGCCTCCA CCAGTGATTC AGGCCAGCAG 
GTGACCCAGC CATCTGAAAT TGAGCAAGGA TTCCTTCCTA AAGGCTGGGA AGTCCGGCAT 
GCACCAAATG GGAGGCCTTT CTTTATTGAC CACAACACTA AAACCACCAC CTGGGAAGAT 
CCAAGATTGA AAATTCCAGC CCATCTGAGA GGAAAGACAT CACTTGATAC TTCCAATGAT 
CTAGGGCCTT TACCTCCAGG ATGGGAAGAG AGAACTCACA CAGATGGAAG AATCTTCTAC 
ATAAATCACA ATATAAAAAG AACACAATGG GAAGATCCTC GGTTGGAGAA TGTAGCAATA 
ACTGGACCAG CAGTGCCCTA CTCCAGGGAT TACAAAAGAA AGTATGAGTT CTTCCGAAGA 
AAGTTGAAGA AGCAGAATGA CATTCCAAAC AAATTTGAAA TGAAACTTCG CCGAGCAACT 
GTTCTTGAAG ACTCTTACCG GAGAATTATG GGTGTCAAGA GAGCAGACTT CCTGAAGGCT 
CGACTGTGGA TTGAGTTTGA TGGTGAAAAG GGATTGGATT ATGGAGGAGT TGCCAGAGAA 
TGGTTCTTCC TGATCTCAAA GGAAATGTTT AACCCTTATT ATGGGTTGTT TGAATATTCT 
GCTACGGACA ATTATACCCT ACAGATAAAT CCAAACTCTG GATTGTGTAA CGAAGATCAC 
CTCTCTTACT TCAAGTTTAT TGGTCGGGTA GCTGGAATGG CAGTTTATCA TGGCAAACTG 
TTGGATGGTT TTTTCATCCG CCCATTTTAC AAGATGATGC TTCACAAACC AATAACCCTT 
CATGATATGG AATCTGTGGA TAGTGAATAT TACAATTCCC TAAGATGGAT TCTTGAAAAT 
GACCCAACAG AATTGGACCT CAGGTTTATC ATAGATGAAG AACTTTTTGG ACAGACACAT 
CAACATGAGC TGAAAAATGG TGGATCAGAA ATAGTTGTCA CCAATAAGAA CAAAAAGGAA 
TATATTTATC TTGTAATACA ATGGCGATTT GTAAACCGAA TCCAGAAGCA AATGGCTGCT 
TTTAAAGAGG GATTCTTTGA ACTAATACCA CAGGATCTCA TCAAAATTTT TGATGAAAAT 
GAACTAGAGC TTCTTATGTG TGGACTGGGA GATGTTGATG TGAATGACTG GAGGGAACAT 
ACAAAGTATA AAAATGGCTA CAGTGCAAAT CATCAGGTTA TACAGTGGTT TTGGAAGGCT 
GTTTTAATGA TGGATTCAGA AAAAAGAATA AGATTACTTC AGTTTGTCAC TGGCACATCT 
CGGGTGCCTA TGAATGGATT TGCTGAACTA TACGGTTCAA ATGGACCACA GTCATTTACA 
GTTGAACAGT GGGGTACTCC TGAAAAGCTG CCAAGAGCTC ATACCTGTTT TAATCGCCTG 
GACTTGCCAC CTTATGAATC ATTTGAAGAA TTATGGGATA AACTTCAGAT GGCAATTGAA 
AACACCCAGG GCTTTGATGG AGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( XM_011521625.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519927.1 Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu59238
Clone ID Related Accession (Same CDS sequence)	XM_011521625.2 , XM_011521626.1 , XM_011521625.3
Accession Version	XM_011521625.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521625.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG TTCTTACAAG TGTGCAAACA AAAACCATTA AAAAGAGTTT GAATCCAAAG 
TGGAATGAAG AAATATTATT CAGAGTTCAT CCTCAGCAGC ACCGGCTTCT TTTTGAAGTG 
TTTGACGAAA ACCGATTGAC AAGAGATGAT TTCCTAGGTC AAGTGGATGT TCCACTTTAT 
CCATTACCGA CAGAAAATCC AAGATTGGAG AGACCATATA CATTTAAGGA TTTTGTTCTT 
CATCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATCTGA GACTAAAAAT GACTTATTTA 
CCTAAAACCA GTGGCTCAGA AGATGATAAT GCAGAACAGG CTGAGGAATT AGAGCCTGGC 
TGGGTTGTTT TGGACCAACC AGATGCTGCT TGCCATTTGC AGCAACAACA AGAACCTTCT 
CCTCTACCTC CAGGGTGGGA AGAGAGGCAG GATATCCTTG GAAGGACCTA TTATGTAAAC 
CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCCCTC AGGACAACCT AACAGATGCT 
GAGAATGGCA ACATTCAACT GCAAGCACAA CGTGCATTTA CCACCAGGCG GCAGATATCC 
GAGGAAACAG AAAGTGTTGA CAACCGAGAG TCTTCCGAGA ACTGGGAAAT TATAAGAGAA 
GATGAAGCCA CCATGTATAG CAACCAGGCC TTCCCATCAC CTCCACCGTC AAGTAACTTG 
GATGTTCCAA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACCATTTT TGGAAATTCA 
GCCGTGAGCC AGCCAGCATC GAGCTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC 
TATACTTTTG AGGAACAACC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA 
CCAGGTTGGG AAGAAAAACA AGATGAAAGA GGAAGATCAT ATTATGTAGA TCACAATTCC 
AGAACGACTA CTTGGACAAA GCCCACTGTA CAGGCCACAG TGGAGACCAG TCAGCTGACC 
TCAAGCCAGA GTTCTGCAGG CCCTCAATCA CAAGCCTCCA CCAGTGATTC AGGCCAGCAG 
GTGACCCAGC CATCTGAAAT TGAGCAAGGA TTCCTTCCTA AAGGCTGGGA AGTCCGGCAT 
GCACCAAATG GGAGGCCTTT CTTTATTGAC CACAACACTA AAACCACCAC CTGGGAAGAT 
CCAAGATTGA AAATTCCAGC CCATCTGAGA GGAAAGACAT CACTTGATAC TTCCAATGAT 
CTAGGGCCTT TACCTCCAGG ATGGGAAGAG AGAACTCACA CAGATGGAAG AATCTTCTAC 
ATAAATCACA ATATAAAAAG AACACAATGG GAAGATCCTC GGTTGGAGAA TGTAGCAATA 
ACTGGACCAG CAGTGCCCTA CTCCAGGGAT TACAAAAGAA AGTATGAGTT CTTCCGAAGA 
AAGTTGAAGA AGCAGAATGA CATTCCAAAC AAATTTGAAA TGAAACTTCG CCGAGCAACT 
GTTCTTGAAG ACTCTTACCG GAGAATTATG GGTGTCAAGA GAGCAGACTT CCTGAAGGCT 
CGACTGTGGA TTGAGTTTGA TGGTGAAAAG GGATTGGATT ATGGAGGAGT TGCCAGAGAA 
TGGTTCTTCC TGATCTCAAA GGAAATGTTT AACCCTTATT ATGGGTTGTT TGAATATTCT 
GCTACGGACA ATTATACCCT ACAGATAAAT CCAAACTCTG GATTGTGTAA CGAAGATCAC 
CTCTCTTACT TCAAGTTTAT TGGTCGGGTA GCTGGAATGG CAGTTTATCA TGGCAAACTG 
TTGGATGGTT TTTTCATCCG CCCATTTTAC AAGATGATGC TTCACAAACC AATAACCCTT 
CATGATATGG AATCTGTGGA TAGTGAATAT TACAATTCCC TAAGATGGAT TCTTGAAAAT 
GACCCAACAG AATTGGACCT CAGGTTTATC ATAGATGAAG AACTTTTTGG ACAGACACAT 
CAACATGAGC TGAAAAATGG TGGATCAGAA ATAGTTGTCA CCAATAAGAA CAAAAAGGAA 
TATATTTATC TTGTAATACA ATGGCGATTT GTAAACCGAA TCCAGAAGCA AATGGCTGCT 
TTTAAAGAGG GATTCTTTGA ACTAATACCA CAGGATCTCA TCAAAATTTT TGATGAAAAT 
GAACTAGAGC TTCTTATGTG TGGACTGGGA GATGTTGATG TGAATGACTG GAGGGAACAT 
ACAAAGTATA AAAATGGCTA CAGTGCAAAT CATCAGGTTA TACAGTGGTT TTGGAAGGCT 
GTTTTAATGA TGGATTCAGA AAAAAGAATA AGATTACTTC AGTTTGTCAC TGGCACATCT 
CGGGTGCCTA TGAATGGATT TGCTGAACTA TACGGTTCAA ATGGACCACA GTCATTTACA 
GTTGAACAGT GGGGTACTCC TGAAAAGCTG CCAAGAGCTC ATACCTGTTT TAATCGCCTG 
GACTTGCCAC CTTATGAATC ATTTGAAGAA TTATGGGATA AACTTCAGAT GGCAATTGAA 
AACACCCAGG GCTTTGATGG AGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519927.1
CDS	282..2831
Translation	MNGVLTSVQTKTIKKSLNPKWNEEILFRVHPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	XM_011521625.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521625.3

ORF Insert Sequence:

ATGAATGGAG TTCTTACAAG TGTGCAAACA AAAACCATTA AAAAGAGTTT GAATCCAAAG 
TGGAATGAAG AAATATTATT CAGAGTTCAT CCTCAGCAGC ACCGGCTTCT TTTTGAAGTG 
TTTGACGAAA ACCGATTGAC AAGAGATGAT TTCCTAGGTC AAGTGGATGT TCCACTTTAT 
CCATTACCGA CAGAAAATCC AAGATTGGAG AGACCATATA CATTTAAGGA TTTTGTTCTT 
CATCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATCTGA GACTAAAAAT GACTTATTTA 
CCTAAAACCA GTGGCTCAGA AGATGATAAT GCAGAACAGG CTGAGGAATT AGAGCCTGGC 
TGGGTTGTTT TGGACCAACC AGATGCTGCT TGCCATTTGC AGCAACAACA AGAACCTTCT 
CCTCTACCTC CAGGGTGGGA AGAGAGGCAG GATATCCTTG GAAGGACCTA TTATGTAAAC 
CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCCCTC AGGACAACCT AACAGATGCT 
GAGAATGGCA ACATTCAACT GCAAGCACAA CGTGCATTTA CCACCAGGCG GCAGATATCC 
GAGGAAACAG AAAGTGTTGA CAACCGAGAG TCTTCCGAGA ACTGGGAAAT TATAAGAGAA 
GATGAAGCCA CCATGTATAG CAACCAGGCC TTCCCATCAC CTCCACCGTC AAGTAACTTG 
GATGTTCCAA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACCATTTT TGGAAATTCA 
GCCGTGAGCC AGCCAGCATC GAGCTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC 
TATACTTTTG AGGAACAACC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA 
CCAGGTTGGG AAGAAAAACA AGATGAAAGA GGAAGATCAT ATTATGTAGA TCACAATTCC 
AGAACGACTA CTTGGACAAA GCCCACTGTA CAGGCCACAG TGGAGACCAG TCAGCTGACC 
TCAAGCCAGA GTTCTGCAGG CCCTCAATCA CAAGCCTCCA CCAGTGATTC AGGCCAGCAG 
GTGACCCAGC CATCTGAAAT TGAGCAAGGA TTCCTTCCTA AAGGCTGGGA AGTCCGGCAT 
GCACCAAATG GGAGGCCTTT CTTTATTGAC CACAACACTA AAACCACCAC CTGGGAAGAT 
CCAAGATTGA AAATTCCAGC CCATCTGAGA GGAAAGACAT CACTTGATAC TTCCAATGAT 
CTAGGGCCTT TACCTCCAGG ATGGGAAGAG AGAACTCACA CAGATGGAAG AATCTTCTAC 
ATAAATCACA ATATAAAAAG AACACAATGG GAAGATCCTC GGTTGGAGAA TGTAGCAATA 
ACTGGACCAG CAGTGCCCTA CTCCAGGGAT TACAAAAGAA AGTATGAGTT CTTCCGAAGA 
AAGTTGAAGA AGCAGAATGA CATTCCAAAC AAATTTGAAA TGAAACTTCG CCGAGCAACT 
GTTCTTGAAG ACTCTTACCG GAGAATTATG GGTGTCAAGA GAGCAGACTT CCTGAAGGCT 
CGACTGTGGA TTGAGTTTGA TGGTGAAAAG GGATTGGATT ATGGAGGAGT TGCCAGAGAA 
TGGTTCTTCC TGATCTCAAA GGAAATGTTT AACCCTTATT ATGGGTTGTT TGAATATTCT 
GCTACGGACA ATTATACCCT ACAGATAAAT CCAAACTCTG GATTGTGTAA CGAAGATCAC 
CTCTCTTACT TCAAGTTTAT TGGTCGGGTA GCTGGAATGG CAGTTTATCA TGGCAAACTG 
TTGGATGGTT TTTTCATCCG CCCATTTTAC AAGATGATGC TTCACAAACC AATAACCCTT 
CATGATATGG AATCTGTGGA TAGTGAATAT TACAATTCCC TAAGATGGAT TCTTGAAAAT 
GACCCAACAG AATTGGACCT CAGGTTTATC ATAGATGAAG AACTTTTTGG ACAGACACAT 
CAACATGAGC TGAAAAATGG TGGATCAGAA ATAGTTGTCA CCAATAAGAA CAAAAAGGAA 
TATATTTATC TTGTAATACA ATGGCGATTT GTAAACCGAA TCCAGAAGCA AATGGCTGCT 
TTTAAAGAGG GATTCTTTGA ACTAATACCA CAGGATCTCA TCAAAATTTT TGATGAAAAT 
GAACTAGAGC TTCTTATGTG TGGACTGGGA GATGTTGATG TGAATGACTG GAGGGAACAT 
ACAAAGTATA AAAATGGCTA CAGTGCAAAT CATCAGGTTA TACAGTGGTT TTGGAAGGCT 
GTTTTAATGA TGGATTCAGA AAAAAGAATA AGATTACTTC AGTTTGTCAC TGGCACATCT 
CGGGTGCCTA TGAATGGATT TGCTGAACTA TACGGTTCAA ATGGACCACA GTCATTTACA 
GTTGAACAGT GGGGTACTCC TGAAAAGCTG CCAAGAGCTC ATACCTGTTT TAATCGCCTG 
GACTTGCCAC CTTATGAATC ATTTGAAGAA TTATGGGATA AACTTCAGAT GGCAATTGAA 
AACACCCAGG GCTTTGATGG AGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( XM_011521627.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519929.1 Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X4, mRNA.

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CloneID	OHu59239
Clone ID Related Accession (Same CDS sequence)	XM_011521627.1
Accession Version	XM_011521627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGT GTGAACAGGT TCATCCTCAG CAGCACCGGC TTCTTTTTGA AGTGTTTGAC 
GAAAACCGAT TGACAAGAGA TGATTTCCTA GGTCAAGTGG ATGTTCCACT TTATCCATTA 
CCGACAGAAA ATCCAAGATT GGAGAGACCA TATACATTTA AGGATTTTGT TCTTCATCCA 
AGAAGTCACA AATCAAGAGT TAAAGGTTAT CTGAGACTAA AAATGACTTA TTTACCTAAA 
ACCAGTGGCT CAGAAGATGA TAATGCAGAA CAGGCTGAGG AATTAGAGCC TGGCTGGGTT 
GTTTTGGACC AACCAGATGC TGCTTGCCAT TTGCAGCAAC AACAAGAACC TTCTCCTCTA 
CCTCCAGGGT GGGAAGAGAG GCAGGATATC CTTGGAAGGA CCTATTATGT AAACCATGAA 
TCTAGAAGAA CACAGTGGAA AAGACCAACC CCTCAGGACA ACCTAACAGA TGCTGAGAAT 
GGCAACATTC AACTGCAAGC ACAACGTGCA TTTACCACCA GGCGGCAGAT ATCCGAGGAA 
ACAGAAAGTG TTGACAACCG AGAGTCTTCC GAGAACTGGG AAATTATAAG AGAAGATGAA 
GCCACCATGT ATAGCAACCA GGCCTTCCCA TCACCTCCAC CGTCAAGTAA CTTGGATGTT 
CCAACTCATC TTGCAGAAGA ATTGAATGCC AGACTCACCA TTTTTGGAAA TTCAGCCGTG 
AGCCAGCCAG CATCGAGCTC AAATCATTCC AGCAGAAGAG GCAGCTTACA AGCCTATACT 
TTTGAGGAAC AACCTACACT TCCTGTGCTT TTGCCTACTT CATCTGGATT ACCACCAGGT 
TGGGAAGAAA AACAAGATGA AAGAGGAAGA TCATATTATG TAGATCACAA TTCCAGAACG 
ACTACTTGGA CAAAGCCCAC TGTACAGGCC ACAGTGGAGA CCAGTCAGCT GACCTCAAGC 
CAGAGTTCTG CAGGCCCTCA ATCACAAGCC TCCACCAGTG ATTCAGGCCA GCAGGTGACC 
CAGCCATCTG AAATTGAGCA AGGATTCCTT CCTAAAGGCT GGGAAGTCCG GCATGCACCA 
AATGGGAGGC CTTTCTTTAT TGACCACAAC ACTAAAACCA CCACCTGGGA AGATCCAAGA 
TTGAAAATTC CAGCCCATCT GAGAGGAAAG ACATCACTTG ATACTTCCAA TGATCTAGGG 
CCTTTACCTC CAGGATGGGA AGAGAGAACT CACACAGATG GAAGAATCTT CTACATAAAT 
CACAATATAA AAAGAACACA ATGGGAAGAT CCTCGGTTGG AGAATGTAGC AATAACTGGA 
CCAGCAGTGC CCTACTCCAG GGATTACAAA AGAAAGTATG AGTTCTTCCG AAGAAAGTTG 
AAGAAGCAGA ATGACATTCC AAACAAATTT GAAATGAAAC TTCGCCGAGC AACTGTTCTT 
GAAGACTCTT ACCGGAGAAT TATGGGTGTC AAGAGAGCAG ACTTCCTGAA GGCTCGACTG 
TGGATTGAGT TTGATGGTGA AAAGGGATTG GATTATGGAG GAGTTGCCAG AGAATGGTTC 
TTCCTGATCT CAAAGGAAAT GTTTAACCCT TATTATGGGT TGTTTGAATA TTCTGCTACG 
GACAATTATA CCCTACAGAT AAATCCAAAC TCTGGATTGT GTAACGAAGA TCACCTCTCT 
TACTTCAAGT TTATTGGTCG GGTAGCTGGA ATGGCAGTTT ATCATGGCAA ACTGTTGGAT 
GGTTTTTTCA TCCGCCCATT TTACAAGATG ATGCTTCACA AACCAATAAC CCTTCATGAT 
ATGGAATCTG TGGATAGTGA ATATTACAAT TCCCTAAGAT GGATTCTTGA AAATGACCCA 
ACAGAATTGG ACCTCAGGTT TATCATAGAT GAAGAACTTT TTGGACAGAC ACATCAACAT 
GAGCTGAAAA ATGGTGGATC AGAAATAGTT GTCACCAATA AGAACAAAAA GGAATATATT 
TATCTTGTAA TACAATGGCG ATTTGTAAAC CGAATCCAGA AGCAAATGGC TGCTTTTAAA 
GAGGGATTCT TTGAACTAAT ACCACAGGAT CTCATCAAAA TTTTTGATGA AAATGAACTA 
GAGCTTCTTA TGTGTGGACT GGGAGATGTT GATGTGAATG ACTGGAGGGA ACATACAAAG 
TATAAAAATG GCTACAGTGC AAATCATCAG GTTATACAGT GGTTTTGGAA GGCTGTTTTA 
ATGATGGATT CAGAAAAAAG AATAAGATTA CTTCAGTTTG TCACTGGCAC ATCTCGGGTG 
CCTATGAATG GATTTGCTGA ACTATACGGT TCAAATGGAC CACAGTCATT TACAGTTGAA 
CAGTGGGGTA CTCCTGAAAA GCTGCCAAGA GCTCATACCT GTTTTAATCG CCTGGACTTG 
CCACCTTATG AATCATTTGA AGAATTATGG GATAAACTTC AGATGGCAAT TGAAAACACC 
CAGGGCTTTG ATGGAGTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519929.1
CDS	16..2499
Translation	MDQCEQVHPQQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	XM_011521627.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521627.1

ORF Insert Sequence:

ATGGACCAGT GTGAACAGGT TCATCCTCAG CAGCACCGGC TTCTTTTTGA AGTGTTTGAC 
GAAAACCGAT TGACAAGAGA TGATTTCCTA GGTCAAGTGG ATGTTCCACT TTATCCATTA 
CCGACAGAAA ATCCAAGATT GGAGAGACCA TATACATTTA AGGATTTTGT TCTTCATCCA 
AGAAGTCACA AATCAAGAGT TAAAGGTTAT CTGAGACTAA AAATGACTTA TTTACCTAAA 
ACCAGTGGCT CAGAAGATGA TAATGCAGAA CAGGCTGAGG AATTAGAGCC TGGCTGGGTT 
GTTTTGGACC AACCAGATGC TGCTTGCCAT TTGCAGCAAC AACAAGAACC TTCTCCTCTA 
CCTCCAGGGT GGGAAGAGAG GCAGGATATC CTTGGAAGGA CCTATTATGT AAACCATGAA 
TCTAGAAGAA CACAGTGGAA AAGACCAACC CCTCAGGACA ACCTAACAGA TGCTGAGAAT 
GGCAACATTC AACTGCAAGC ACAACGTGCA TTTACCACCA GGCGGCAGAT ATCCGAGGAA 
ACAGAAAGTG TTGACAACCG AGAGTCTTCC GAGAACTGGG AAATTATAAG AGAAGATGAA 
GCCACCATGT ATAGCAACCA GGCCTTCCCA TCACCTCCAC CGTCAAGTAA CTTGGATGTT 
CCAACTCATC TTGCAGAAGA ATTGAATGCC AGACTCACCA TTTTTGGAAA TTCAGCCGTG 
AGCCAGCCAG CATCGAGCTC AAATCATTCC AGCAGAAGAG GCAGCTTACA AGCCTATACT 
TTTGAGGAAC AACCTACACT TCCTGTGCTT TTGCCTACTT CATCTGGATT ACCACCAGGT 
TGGGAAGAAA AACAAGATGA AAGAGGAAGA TCATATTATG TAGATCACAA TTCCAGAACG 
ACTACTTGGA CAAAGCCCAC TGTACAGGCC ACAGTGGAGA CCAGTCAGCT GACCTCAAGC 
CAGAGTTCTG CAGGCCCTCA ATCACAAGCC TCCACCAGTG ATTCAGGCCA GCAGGTGACC 
CAGCCATCTG AAATTGAGCA AGGATTCCTT CCTAAAGGCT GGGAAGTCCG GCATGCACCA 
AATGGGAGGC CTTTCTTTAT TGACCACAAC ACTAAAACCA CCACCTGGGA AGATCCAAGA 
TTGAAAATTC CAGCCCATCT GAGAGGAAAG ACATCACTTG ATACTTCCAA TGATCTAGGG 
CCTTTACCTC CAGGATGGGA AGAGAGAACT CACACAGATG GAAGAATCTT CTACATAAAT 
CACAATATAA AAAGAACACA ATGGGAAGAT CCTCGGTTGG AGAATGTAGC AATAACTGGA 
CCAGCAGTGC CCTACTCCAG GGATTACAAA AGAAAGTATG AGTTCTTCCG AAGAAAGTTG 
AAGAAGCAGA ATGACATTCC AAACAAATTT GAAATGAAAC TTCGCCGAGC AACTGTTCTT 
GAAGACTCTT ACCGGAGAAT TATGGGTGTC AAGAGAGCAG ACTTCCTGAA GGCTCGACTG 
TGGATTGAGT TTGATGGTGA AAAGGGATTG GATTATGGAG GAGTTGCCAG AGAATGGTTC 
TTCCTGATCT CAAAGGAAAT GTTTAACCCT TATTATGGGT TGTTTGAATA TTCTGCTACG 
GACAATTATA CCCTACAGAT AAATCCAAAC TCTGGATTGT GTAACGAAGA TCACCTCTCT 
TACTTCAAGT TTATTGGTCG GGTAGCTGGA ATGGCAGTTT ATCATGGCAA ACTGTTGGAT 
GGTTTTTTCA TCCGCCCATT TTACAAGATG ATGCTTCACA AACCAATAAC CCTTCATGAT 
ATGGAATCTG TGGATAGTGA ATATTACAAT TCCCTAAGAT GGATTCTTGA AAATGACCCA 
ACAGAATTGG ACCTCAGGTT TATCATAGAT GAAGAACTTT TTGGACAGAC ACATCAACAT 
GAGCTGAAAA ATGGTGGATC AGAAATAGTT GTCACCAATA AGAACAAAAA GGAATATATT 
TATCTTGTAA TACAATGGCG ATTTGTAAAC CGAATCCAGA AGCAAATGGC TGCTTTTAAA 
GAGGGATTCT TTGAACTAAT ACCACAGGAT CTCATCAAAA TTTTTGATGA AAATGAACTA 
GAGCTTCTTA TGTGTGGACT GGGAGATGTT GATGTGAATG ACTGGAGGGA ACATACAAAG 
TATAAAAATG GCTACAGTGC AAATCATCAG GTTATACAGT GGTTTTGGAA GGCTGTTTTA 
ATGATGGATT CAGAAAAAAG AATAAGATTA CTTCAGTTTG TCACTGGCAC ATCTCGGGTG 
CCTATGAATG GATTTGCTGA ACTATACGGT TCAAATGGAC CACAGTCATT TACAGTTGAA 
CAGTGGGGTA CTCCTGAAAA GCTGCCAAGA GCTCATACCT GTTTTAATCG CCTGGACTTG 
CCACCTTATG AATCATTTGA AGAATTATGG GATAAACTTC AGATGGCAAT TGAAAACACC 
CAGGGCTTTG ATGGAGTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( NM_001284340.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271269.1 Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu20676
Clone ID Related Accession (Same CDS sequence)	NM_001284340.1
Accession Version	NM_001284340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832063.1, BC136605.1, AL832359.1, BC144285.1 and AC009997.7. Transcript Variant: This variant (5) contains two alternate in-frame exons, compared to variant 2. The encoded isoform (5) is longer than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832359.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA CCCTTTGTCA 
GAAACCTCCG GAGATGACTT GGATAGCCAT GTTCACATGT GCTTCAAAAG ACCAACACGG 
ATTTCAACGT CTAACGTTGT TCAAATGAAG CTGACTCCCA GACAGACTGC ACTAGCTCCG 
TTAATAAAGG AAAACGTTCA GTCTCAAGAA AGATCATCTG TTCCCTCATC TGAAAATGTT 
AATAAAAAGA GCAGCTGTCT ACAGATTTCA CTACAGCCAA CAAGGTACAG TGGATATCTT 
CAGTCTAGCA ATGTCTTAGC TGATAGTGAT GATGCTTCGT TTACTTGTAT CTTGAAGGAT 
GGTATTTACA GTAGTGCTGT GGTCGATAAT GAATTGAATG CTGTGAATGA TGGTCACCTT 
GTAAGCAGTC CAGCCATTTG TAGTGGTAGC CTTAGTAACT TTTCAACCAG TGATAATGGG 
TCTTACAGCA GCAACGGTAG TGATTTTGGG TCATGTGCAA GTATCACAAG TGGAGGTTCA 
TATACTAACA GTGTCATCAG TGACAGTAGT AGTTATACTT TTCCACCAAG TGATGATACT 
TTTTTGGGTG GAAACTTACC TTCTGACAGC ACCTCCAATA GAAGTGTGCC AAACAGGAAT 
ACTACTCCTT GTGAAATTTT TTCAAGAAGT ACAAGTACAG ATCCTTTTGT CCAGGATGAC 
TTGGAACATG GATTAGAGAT TATGAAATTG CCAGTGAGCA GGAACACAAA AATTCCACTA 
AAACGTTACT CCTCCTTAGT CATTTTTCCT AGGAGTCCTT CAACTACCCG ACCGACTTCT 
CCAACAAGTC TGTGTACTCT TCTGAGCAAA GGATCCTATC AAACTTCACA CCAGTTTATT 
ATTTCTCCTA GTGAAATTGC ACATAATGAG GATGGCACTA GTGCTAAAGG ATTTCTTTCA 
ACAGCTGTCA ATGGACTTCG GTTATCTAAA ACAATTTGTA CCCCCGGAGA AGTAAGAGAC 
ATACGGCCGC TTCACAGGAA GGGCTCGTTA CAGAAGAAAA TTGTTCTTTC GAATAATACT 
CCCAGACAGA CTGTCTGTGA AAAGTCATCT GAAGGATATT CTTGTGTTTC AGTGCATTTC 
ACCCAACGAA AAGCAGCTAC ATTAGACTGT GAAACAACAA ATGGTGATTG TAAACCAGAA 
ATGTCAGAAA TTAAGCTTAA TTCTGATTCA GAGTATATTA AGCTCATGCA TAGGACATCT 
GCATGTTTGC CATCCTCCCA AAATGTAGAT TGTCAAATAA ATATCAATGG AGAATTGGAA 
AGACCACATT CACAGATGAA CAAAAACCAT GGTATTTTAC GAAGAAGTAT TTCATTGGGA 
GGAGCTTATC CAAATATTTC CTGTCTATCC AGCCTTAAGC ACAATTGTTC TAAAGGGGGA 
CCATCTCAGT TACTCATAAA GTTTGCATCT GGAAATGAAG GTAAAGTGGA TAATTTATCA 
AGAGACAGCA ACAGAGATTG CACAAATGAA CTGTCTAATT CTTGCAAGAC AGAAAATCCA 
AGATTGGAGA GACCATATAC ATTTAAGGAT TTTGTTCTTC ATCCAAGAAG TCACAAATCA 
AGAGTTAAAG GTTATCTGAG ACTAAAAATG ACTTATTTAC CTAAAACCAG TGGCTCAGAA 
GATGATAATG CAGAACAGGC TGAGGAATTA GAGCCTGGCT GGGTTGTTTT GGACCAACCA 
GATGCTGCTT GCCATTTGCA GCAACAACAA GAACCTTCTC CTCTACCTCC AGGGTGGGAA 
GAGAGGCAGG ATATCCTTGG AAGGACCTAT TATGTAAACC ATGAATCTAG AAGAACACAG 
TGGAAAAGAC CAACCCCTCA GGACAACCTA ACAGATGCTG AGAATGGCAA CATTCAACTG 
CAAGCACAAC GTGCATTTAC CACCAGGCGG CAGATATCCG AGGAAACAGA AAGTGTTGAC 
AACCGAGAGT CTTCCGAGAA CTGGGAAATT ATAAGAGAAG ATGAAGCCAC CATGTATAGC 
AACCAGGCCT TCCCATCACC TCCACCGTCA AGTAACTTGG ATGTTCCAAC TCATCTTGCA 
GAAGAATTGA ATGCCAGACT CACCATTTTT GGAAATTCAG CCGTGAGCCA GCCAGCATCG 
AGCTCAAATC ATTCCAGCAG AAGAGGCAGC TTACAAGCCT ATACTTTTGA GGAACAACCT 
ACACTTCCTG TGCTTTTGCC TACTTCATCT GGATTACCAC CAGGTTGGGA AGAAAAACAA 
GATGAAAGAG GAAGATCATA TTATGTAGAT CACAATTCCA GAACGACTAC TTGGACAAAG 
CCCACTGTAC AGGCCACAGT GGAGACCAGT CAGCTGACCT CAAGCCAGAG TTCTGCAGGC 
CCTCAATCAC AAGCCTCCAC CAGTGATTCA GGCCAGCAGG TGACCCAGCC ATCTGAAATT 
GAGCAAGGAT TCCTTCCTAA AGGCTGGGAA GTCCGGCATG CACCAAATGG GAGGCCTTTC 
TTTATTGACC ACAACACTAA AACCACCACC TGGGAAGATC CAAGATTGAA AATTCCAGCC 
CATCTGAGAG GAAAGACATC ACTTGATACT TCCAATGATC TAGGGCCTTT ACCTCCAGGA 
TGGGAAGAGA GAACTCACAC AGATGGAAGA ATCTTCTACA TAAATCACAA TATAAAAAGA 
ACACAATGGG AAGATCCTCG GTTGGAGAAT GTAGCAATAA CTGGACCAGC AGTGCCCTAC 
TCCAGGGATT ACAAAAGAAA GTATGAGTTC TTCCGAAGAA AGTTGAAGAA GCAGAATGAC 
ATTCCAAACA AATTTGAAAT GAAACTTCGC CGAGCAACTG TTCTTGAAGA CTCTTACCGG 
AGAATTATGG GTGTCAAGAG AGCAGACTTC CTGAAGGCTC GACTGTGGAT TGAGTTTGAT 
GGTGAAAAGG GATTGGATTA TGGAGGAGTT GCCAGAGAAT GGTTCTTCCT GATCTCAAAG 
GAAATGTTTA ACCCTTATTA TGGGTTGTTT GAATATTCTG CTACGGACAA TTATACCCTA 
CAGATAAATC CAAACTCTGG ATTGTGTAAC GAAGATCACC TCTCTTACTT CAAGTTTATT 
GGTCGGGTAG CTGGAATGGC AGTTTATCAT GGCAAACTGT TGGATGGTTT TTTCATCCGC 
CCATTTTACA AGATGATGCT TCACAAACCA ATAACCCTTC ATGATATGGA ATCTGTGGAT 
AGTGAATATT ACAATTCCCT AAGATGGATT CTTGAAAATG ACCCAACAGA ATTGGACCTC 
AGGTTTATCA TAGATGAAGA ACTTTTTGGA CAGACACATC AACATGAGCT GAAAAATGGT 
GGATCAGAAA TAGTTGTCAC CAATAAGAAC AAAAAGGAAT ATATTTATCT TGTAATACAA 
TGGCGATTTG TAAACCGAAT CCAGAAGCAA ATGGCTGCTT TTAAAGAGGG ATTCTTTGAA 
CTAATACCAC AGGATCTCAT CAAAATTTTT GATGAAAATG AACTAGAGCT TCTTATGTGT 
GGACTGGGAG ATGTTGATGT GAATGACTGG AGGGAACATA CAAAGTATAA AAATGGCTAC 
AGTGCAAATC ATCAGGTTAT ACAGTGGTTT TGGAAGGCTG TTTTAATGAT GGATTCAGAA 
AAAAGAATAA GATTACTTCA GTTTGTCACT GGCACATCTC GGGTGCCTAT GAATGGATTT 
GCTGAACTAT ACGGTTCAAA TGGACCACAG TCATTTACAG TTGAACAGTG GGGTACTCCT 
GAAAAGCTGC CAAGAGCTCA TACCTGTTTT AATCGCCTGG ACTTGCCACC TTATGAATCA 
TTTGAAGAAT TATGGGATAA ACTTCAGATG GCAATTGAAA ACACCCAGGG CTTTGATGGA 
GTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271269.1
CDS	301..4209
Translation	MAQSLRLHFAARRSNTYPLSETSGDDLDSHVHMCFKRPTRISTSNVVQMKLTPRQTALAPLIKENVQSQERSSVPSSENVNKKSSCLQISLQPTRYSGYLQSSNVLADSDDASFTCILKDGIYSSAVVDNELNAVNDGHLVSSPAICSGSLSNFSTSDNGSYSSNGSDFGSCASITSGGSYTNSVISDSSSYTFPPSDDTFLGGNLPSDSTSNRSVPNRNTTPCEIFSRSTSTDPFVQDDLEHGLEIMKLPVSRNTKIPLKRYSSLVIFPRSPSTTRPTSPTSLCTLLSKGSYQTSHQFIISPSEIAHNEDGTSAKGFLSTAVNGLRLSKTICTPGEVRDIRPLHRKGSLQKKIVLSNNTPRQTVCEKSSEGYSCVSVHFTQRKAATLDCETTNGDCKPEMSEIKLNSDSEYIKLMHRTSACLPSSQNVDCQININGELERPHSQMNKNHGILRRSISLGGAYPNISCLSSLKHNCSKGGPSQLLIKFASGNEGKVDNLSRDSNRDCTNELSNSCKTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	NM_001284340.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284340.1

ORF Insert Sequence:

ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA CCCTTTGTCA 
GAAACCTCCG GAGATGACTT GGATAGCCAT GTTCACATGT GCTTCAAAAG ACCAACACGG 
ATTTCAACGT CTAACGTTGT TCAAATGAAG CTGACTCCCA GACAGACTGC ACTAGCTCCG 
TTAATAAAGG AAAACGTTCA GTCTCAAGAA AGATCATCTG TTCCCTCATC TGAAAATGTT 
AATAAAAAGA GCAGCTGTCT ACAGATTTCA CTACAGCCAA CAAGGTACAG TGGATATCTT 
CAGTCTAGCA ATGTCTTAGC TGATAGTGAT GATGCTTCGT TTACTTGTAT CTTGAAGGAT 
GGTATTTACA GTAGTGCTGT GGTCGATAAT GAATTGAATG CTGTGAATGA TGGTCACCTT 
GTAAGCAGTC CAGCCATTTG TAGTGGTAGC CTTAGTAACT TTTCAACCAG TGATAATGGG 
TCTTACAGCA GCAACGGTAG TGATTTTGGG TCATGTGCAA GTATCACAAG TGGAGGTTCA 
TATACTAACA GTGTCATCAG TGACAGTAGT AGTTATACTT TTCCACCAAG TGATGATACT 
TTTTTGGGTG GAAACTTACC TTCTGACAGC ACCTCCAATA GAAGTGTGCC AAACAGGAAT 
ACTACTCCTT GTGAAATTTT TTCAAGAAGT ACAAGTACAG ATCCTTTTGT CCAGGATGAC 
TTGGAACATG GATTAGAGAT TATGAAATTG CCAGTGAGCA GGAACACAAA AATTCCACTA 
AAACGTTACT CCTCCTTAGT CATTTTTCCT AGGAGTCCTT CAACTACCCG ACCGACTTCT 
CCAACAAGTC TGTGTACTCT TCTGAGCAAA GGATCCTATC AAACTTCACA CCAGTTTATT 
ATTTCTCCTA GTGAAATTGC ACATAATGAG GATGGCACTA GTGCTAAAGG ATTTCTTTCA 
ACAGCTGTCA ATGGACTTCG GTTATCTAAA ACAATTTGTA CCCCCGGAGA AGTAAGAGAC 
ATACGGCCGC TTCACAGGAA GGGCTCGTTA CAGAAGAAAA TTGTTCTTTC GAATAATACT 
CCCAGACAGA CTGTCTGTGA AAAGTCATCT GAAGGATATT CTTGTGTTTC AGTGCATTTC 
ACCCAACGAA AAGCAGCTAC ATTAGACTGT GAAACAACAA ATGGTGATTG TAAACCAGAA 
ATGTCAGAAA TTAAGCTTAA TTCTGATTCA GAGTATATTA AGCTCATGCA TAGGACATCT 
GCATGTTTGC CATCCTCCCA AAATGTAGAT TGTCAAATAA ATATCAATGG AGAATTGGAA 
AGACCACATT CACAGATGAA CAAAAACCAT GGTATTTTAC GAAGAAGTAT TTCATTGGGA 
GGAGCTTATC CAAATATTTC CTGTCTATCC AGCCTTAAGC ACAATTGTTC TAAAGGGGGA 
CCATCTCAGT TACTCATAAA GTTTGCATCT GGAAATGAAG GTAAAGTGGA TAATTTATCA 
AGAGACAGCA ACAGAGATTG CACAAATGAA CTGTCTAATT CTTGCAAGAC AGAAAATCCA 
AGATTGGAGA GACCATATAC ATTTAAGGAT TTTGTTCTTC ATCCAAGAAG TCACAAATCA 
AGAGTTAAAG GTTATCTGAG ACTAAAAATG ACTTATTTAC CTAAAACCAG TGGCTCAGAA 
GATGATAATG CAGAACAGGC TGAGGAATTA GAGCCTGGCT GGGTTGTTTT GGACCAACCA 
GATGCTGCTT GCCATTTGCA GCAACAACAA GAACCTTCTC CTCTACCTCC AGGGTGGGAA 
GAGAGGCAGG ATATCCTTGG AAGGACCTAT TATGTAAACC ATGAATCTAG AAGAACACAG 
TGGAAAAGAC CAACCCCTCA GGACAACCTA ACAGATGCTG AGAATGGCAA CATTCAACTG 
CAAGCACAAC GTGCATTTAC CACCAGGCGG CAGATATCCG AGGAAACAGA AAGTGTTGAC 
AACCGAGAGT CTTCCGAGAA CTGGGAAATT ATAAGAGAAG ATGAAGCCAC CATGTATAGC 
AACCAGGCCT TCCCATCACC TCCACCGTCA AGTAACTTGG ATGTTCCAAC TCATCTTGCA 
GAAGAATTGA ATGCCAGACT CACCATTTTT GGAAATTCAG CCGTGAGCCA GCCAGCATCG 
AGCTCAAATC ATTCCAGCAG AAGAGGCAGC TTACAAGCCT ATACTTTTGA GGAACAACCT 
ACACTTCCTG TGCTTTTGCC TACTTCATCT GGATTACCAC CAGGTTGGGA AGAAAAACAA 
GATGAAAGAG GAAGATCATA TTATGTAGAT CACAATTCCA GAACGACTAC TTGGACAAAG 
CCCACTGTAC AGGCCACAGT GGAGACCAGT CAGCTGACCT CAAGCCAGAG TTCTGCAGGC 
CCTCAATCAC AAGCCTCCAC CAGTGATTCA GGCCAGCAGG TGACCCAGCC ATCTGAAATT 
GAGCAAGGAT TCCTTCCTAA AGGCTGGGAA GTCCGGCATG CACCAAATGG GAGGCCTTTC 
TTTATTGACC ACAACACTAA AACCACCACC TGGGAAGATC CAAGATTGAA AATTCCAGCC 
CATCTGAGAG GAAAGACATC ACTTGATACT TCCAATGATC TAGGGCCTTT ACCTCCAGGA 
TGGGAAGAGA GAACTCACAC AGATGGAAGA ATCTTCTACA TAAATCACAA TATAAAAAGA 
ACACAATGGG AAGATCCTCG GTTGGAGAAT GTAGCAATAA CTGGACCAGC AGTGCCCTAC 
TCCAGGGATT ACAAAAGAAA GTATGAGTTC TTCCGAAGAA AGTTGAAGAA GCAGAATGAC 
ATTCCAAACA AATTTGAAAT GAAACTTCGC CGAGCAACTG TTCTTGAAGA CTCTTACCGG 
AGAATTATGG GTGTCAAGAG AGCAGACTTC CTGAAGGCTC GACTGTGGAT TGAGTTTGAT 
GGTGAAAAGG GATTGGATTA TGGAGGAGTT GCCAGAGAAT GGTTCTTCCT GATCTCAAAG 
GAAATGTTTA ACCCTTATTA TGGGTTGTTT GAATATTCTG CTACGGACAA TTATACCCTA 
CAGATAAATC CAAACTCTGG ATTGTGTAAC GAAGATCACC TCTCTTACTT CAAGTTTATT 
GGTCGGGTAG CTGGAATGGC AGTTTATCAT GGCAAACTGT TGGATGGTTT TTTCATCCGC 
CCATTTTACA AGATGATGCT TCACAAACCA ATAACCCTTC ATGATATGGA ATCTGTGGAT 
AGTGAATATT ACAATTCCCT AAGATGGATT CTTGAAAATG ACCCAACAGA ATTGGACCTC 
AGGTTTATCA TAGATGAAGA ACTTTTTGGA CAGACACATC AACATGAGCT GAAAAATGGT 
GGATCAGAAA TAGTTGTCAC CAATAAGAAC AAAAAGGAAT ATATTTATCT TGTAATACAA 
TGGCGATTTG TAAACCGAAT CCAGAAGCAA ATGGCTGCTT TTAAAGAGGG ATTCTTTGAA 
CTAATACCAC AGGATCTCAT CAAAATTTTT GATGAAAATG AACTAGAGCT TCTTATGTGT 
GGACTGGGAG ATGTTGATGT GAATGACTGG AGGGAACATA CAAAGTATAA AAATGGCTAC 
AGTGCAAATC ATCAGGTTAT ACAGTGGTTT TGGAAGGCTG TTTTAATGAT GGATTCAGAA 
AAAAGAATAA GATTACTTCA GTTTGTCACT GGCACATCTC GGGTGCCTAT GAATGGATTT 
GCTGAACTAT ACGGTTCAAA TGGACCACAG TCATTTACAG TTGAACAGTG GGGTACTCCT 
GAAAAGCTGC CAAGAGCTCA TACCTGTTTT AATCGCCTGG ACTTGCCACC TTATGAATCA 
TTTGAAGAAT TATGGGATAA ACTTCAGATG GCAATTGAAA ACACCCAGGG CTTTGATGGA 
GTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( NM_198400.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_940682.2 Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 2, mRNA.

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CloneID	OHu21480
Clone ID Related Accession (Same CDS sequence)	NM_198400.3
Accession Version	NM_198400.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832063.1, BC136605.1, AL833300.1, BC035707.1 and AC009997.7. On Sep 28, 2013 this sequence version replaced NM_198400.2. Transcript Variant: This variant (2) encodes isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832063.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA CCCTTTGTCA 
GAAACCTCCG GAGATGACTT GGATAGCCAT GTTCACATGT GCTTCAAAAG ACCAACACGG 
ATTTCAACGT CTAACGTTGT TCAAATGAAG CTGACTCCCA GACAGACTGC ACTAGCTCCG 
TTAATAAAGG AAAACGTTCA GTCTCAAGAA AGATCATCTG TTCCCTCATC TGAAAATGTT 
AATAAAAAGA GCAGCTGTCT ACAGATTTCA CTACAGCCAA CAAGGTACAG TGGATATCTT 
CAGTCTAGCA ATGTCTTAGC TGATAGTGAT GATGCTTCGT TTACTTGTAT CTTGAAGGAT 
GGTATTTACA GTAGTGCTGT GGTCGATAAT GAATTGAATG CTGTGAATGA TGGTCACCTT 
GTAAGCAGTC CAGCCATTTG TAGTGGTAGC CTTAGTAACT TTTCAACCAG TGATAATGGG 
TCTTACAGCA GCAACGGTAG TGATTTTGGG TCATGTGCAA GTATCACAAG TGGAGGTTCA 
TATACTAACA GTGTCATCAG TGACAGTAGT AGTTATACTT TTCCACCAAG TGATGATACT 
TTTTTGGGTG GAAACTTACC TTCTGACAGC ACCTCCAATA GAAGTGTGCC AAACAGGAAT 
ACTACTCCTT GTGAAATTTT TTCAAGAAGT ACAAGTACAG ATCCTTTTGT CCAGGATGAC 
TTGGAACATG GATTAGAGAT TATGAAATTG CCAGTGAGCA GGAACACAAA AATTCCACTA 
AAACGTTACT CCTCCTTAGT CATTTTTCCT AGGAGTCCTT CAACTACCCG ACCGACTTCT 
CCAACAAGTC TGTGTACTCT TCTGAGCAAA GGATCCTATC AAACTTCACA CCAGTTTATT 
ATTTCTCCTA GTGAAATTGC ACATAATGAG GATGGCACTA GTGCTAAAGG ATTTCTTTCA 
ACAGCTGTCA ATGGACTTCG GTTATCTAAA ACAATTTGTA CCCCCGGAGA AGTAAGAGAC 
ATACGGCCGC TTCACAGGAA GGGCTCGTTA CAGAAGAAAA TTGTTCTTTC GAATAATACT 
CCCAGACAGA CTGTCTGTGA AAAGTCATCT GAAGGATATT CTTGTGTTTC AGTGCATTTC 
ACCCAACGAA AAGCAGCTAC ATTAGACTGT GAAACAACAA ATGGTGATTG TAAACCAGAA 
ATGTCAGAAA TTAAGCTTAA TTCTGATTCA GAGTATATTA AGCTCATGCA TAGGACATCT 
GCATGTTTGC CATCCTCCCA AAATGTAGAT TGTCAAATAA ATATCAATGG AGAATTGGAA 
AGACCACATT CACAGATGAA CAAAAACCAT GGTATTTTAC GAAGAAGTAT TTCATTGGGA 
GGAGCTTATC CAAATATTTC CTGTCTATCC AGCCTTAAGC ACAATTGTTC TAAAGGGGGA 
CCATCTCAGT TACTCATAAA GTTTGCATCT GGAAATGAAG GTAAAGTGGA TAATTTATCA 
AGAGACAGCA ACAGAGATTG CACAAATGAA CTGTCTAATT CTTGCAAGCC TGGCTGGGTT 
GTTTTGGACC AACCAGATGC TGCTTGCCAT TTGCAGCAAC AACAAGAACC TTCTCCTCTA 
CCTCCAGGGT GGGAAGAGAG GCAGGATATC CTTGGAAGGA CCTATTATGT AAACCATGAA 
TCTAGAAGAA CACAGTGGAA AAGACCAACC CCTCAGGACA ACCTAACAGA TGCTGAGAAT 
GGCAACATTC AACTGCAAGC ACAACGTGCA TTTACCACCA GGCGGCAGAT ATCCGAGGAA 
ACAGAAAGTG TTGACAACCG AGAGTCTTCC GAGAACTGGG AAATTATAAG AGAAGATGAA 
GCCACCATGT ATAGCAACCA GGCCTTCCCA TCACCTCCAC CGTCAAGTAA CTTGGATGTT 
CCAACTCATC TTGCAGAAGA ATTGAATGCC AGACTCACCA TTTTTGGAAA TTCAGCCGTG 
AGCCAGCCAG CATCGAGCTC AAATCATTCC AGCAGAAGAG GCAGCTTACA AGCCTATACT 
TTTGAGGAAC AACCTACACT TCCTGTGCTT TTGCCTACTT CATCTGGATT ACCACCAGGT 
TGGGAAGAAA AACAAGATGA AAGAGGAAGA TCATATTATG TAGATCACAA TTCCAGAACG 
ACTACTTGGA CAAAGCCCAC TGTACAGGCC ACAGTGGAGA CCAGTCAGCT GACCTCAAGC 
CAGAGTTCTG CAGGCCCTCA ATCACAAGCC TCCACCAGTG ATTCAGGCCA GCAGGTGACC 
CAGCCATCTG AAATTGAGCA AGGATTCCTT CCTAAAGGCT GGGAAGTCCG GCATGCACCA 
AATGGGAGGC CTTTCTTTAT TGACCACAAC ACTAAAACCA CCACCTGGGA AGATCCAAGA 
TTGAAAATTC CAGCCCATCT GAGAGGAAAG ACATCACTTG ATACTTCCAA TGATCTAGGG 
CCTTTACCTC CAGGATGGGA AGAGAGAACT CACACAGATG GAAGAATCTT CTACATAAAT 
CACAATATAA AAAGAACACA ATGGGAAGAT CCTCGGTTGG AGAATGTAGC AATAACTGGA 
CCAGCAGTGC CCTACTCCAG GGATTACAAA AGAAAGTATG AGTTCTTCCG AAGAAAGTTG 
AAGAAGCAGA ATGACATTCC AAACAAATTT GAAATGAAAC TTCGCCGAGC AACTGTTCTT 
GAAGACTCTT ACCGGAGAAT TATGGGTGTC AAGAGAGCAG ACTTCCTGAA GGCTCGACTG 
TGGATTGAGT TTGATGGTGA AAAGGGATTG GATTATGGAG GAGTTGCCAG AGAATGGTTC 
TTCCTGATCT CAAAGGAAAT GTTTAACCCT TATTATGGGT TGTTTGAATA TTCTGCTACG 
GACAATTATA CCCTACAGAT AAATCCAAAC TCTGGATTGT GTAACGAAGA TCACCTCTCT 
TACTTCAAGT TTATTGGTCG GGTAGCTGGA ATGGCAGTTT ATCATGGCAA ACTGTTGGAT 
GGTTTTTTCA TCCGCCCATT TTACAAGATG ATGCTTCACA AACCAATAAC CCTTCATGAT 
ATGGAATCTG TGGATAGTGA ATATTACAAT TCCCTAAGAT GGATTCTTGA AAATGACCCA 
ACAGAATTGG ACCTCAGGTT TATCATAGAT GAAGAACTTT TTGGACAGAC ACATCAACAT 
GAGCTGAAAA ATGGTGGATC AGAAATAGTT GTCACCAATA AGAACAAAAA GGAATATATT 
TATCTTGTAA TACAATGGCG ATTTGTAAAC CGAATCCAGA AGCAAATGGC TGCTTTTAAA 
GAGGGATTCT TTGAACTAAT ACCACAGGAT CTCATCAAAA TTTTTGATGA AAATGAACTA 
GAGCTTCTTA TGTGTGGACT GGGAGATGTT GATGTGAATG ACTGGAGGGA ACATACAAAG 
TATAAAAATG GCTACAGTGC AAATCATCAG GTTATACAGT GGTTTTGGAA GGCTGTTTTA 
ATGATGGATT CAGAAAAAAG AATAAGATTA CTTCAGTTTG TCACTGGCAC ATCTCGGGTG 
CCTATGAATG GATTTGCTGA ACTATACGGT TCAAATGGAC CACAGTCATT TACAGTTGAA 
CAGTGGGGTA CTCCTGAAAA GCTGCCAAGA GCTCATACCT GTTTTAATCG CCTGGACTTG 
CCACCTTATG AATCATTTGA AGAATTATGG GATAAACTTC AGATGGCAAT TGAAAACACC 
CAGGGCTTTG ATGGAGTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_940682.2
CDS	301..4044
Translation	MAQSLRLHFAARRSNTYPLSETSGDDLDSHVHMCFKRPTRISTSNVVQMKLTPRQTALAPLIKENVQSQERSSVPSSENVNKKSSCLQISLQPTRYSGYLQSSNVLADSDDASFTCILKDGIYSSAVVDNELNAVNDGHLVSSPAICSGSLSNFSTSDNGSYSSNGSDFGSCASITSGGSYTNSVISDSSSYTFPPSDDTFLGGNLPSDSTSNRSVPNRNTTPCEIFSRSTSTDPFVQDDLEHGLEIMKLPVSRNTKIPLKRYSSLVIFPRSPSTTRPTSPTSLCTLLSKGSYQTSHQFIISPSEIAHNEDGTSAKGFLSTAVNGLRLSKTICTPGEVRDIRPLHRKGSLQKKIVLSNNTPRQTVCEKSSEGYSCVSVHFTQRKAATLDCETTNGDCKPEMSEIKLNSDSEYIKLMHRTSACLPSSQNVDCQININGELERPHSQMNKNHGILRRSISLGGAYPNISCLSSLKHNCSKGGPSQLLIKFASGNEGKVDNLSRDSNRDCTNELSNSCKPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	NM_198400.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198400.3

ORF Insert Sequence:

ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA CCCTTTGTCA 
GAAACCTCCG GAGATGACTT GGATAGCCAT GTTCACATGT GCTTCAAAAG ACCAACACGG 
ATTTCAACGT CTAACGTTGT TCAAATGAAG CTGACTCCCA GACAGACTGC ACTAGCTCCG 
TTAATAAAGG AAAACGTTCA GTCTCAAGAA AGATCATCTG TTCCCTCATC TGAAAATGTT 
AATAAAAAGA GCAGCTGTCT ACAGATTTCA CTACAGCCAA CAAGGTACAG TGGATATCTT 
CAGTCTAGCA ATGTCTTAGC TGATAGTGAT GATGCTTCGT TTACTTGTAT CTTGAAGGAT 
GGTATTTACA GTAGTGCTGT GGTCGATAAT GAATTGAATG CTGTGAATGA TGGTCACCTT 
GTAAGCAGTC CAGCCATTTG TAGTGGTAGC CTTAGTAACT TTTCAACCAG TGATAATGGG 
TCTTACAGCA GCAACGGTAG TGATTTTGGG TCATGTGCAA GTATCACAAG TGGAGGTTCA 
TATACTAACA GTGTCATCAG TGACAGTAGT AGTTATACTT TTCCACCAAG TGATGATACT 
TTTTTGGGTG GAAACTTACC TTCTGACAGC ACCTCCAATA GAAGTGTGCC AAACAGGAAT 
ACTACTCCTT GTGAAATTTT TTCAAGAAGT ACAAGTACAG ATCCTTTTGT CCAGGATGAC 
TTGGAACATG GATTAGAGAT TATGAAATTG CCAGTGAGCA GGAACACAAA AATTCCACTA 
AAACGTTACT CCTCCTTAGT CATTTTTCCT AGGAGTCCTT CAACTACCCG ACCGACTTCT 
CCAACAAGTC TGTGTACTCT TCTGAGCAAA GGATCCTATC AAACTTCACA CCAGTTTATT 
ATTTCTCCTA GTGAAATTGC ACATAATGAG GATGGCACTA GTGCTAAAGG ATTTCTTTCA 
ACAGCTGTCA ATGGACTTCG GTTATCTAAA ACAATTTGTA CCCCCGGAGA AGTAAGAGAC 
ATACGGCCGC TTCACAGGAA GGGCTCGTTA CAGAAGAAAA TTGTTCTTTC GAATAATACT 
CCCAGACAGA CTGTCTGTGA AAAGTCATCT GAAGGATATT CTTGTGTTTC AGTGCATTTC 
ACCCAACGAA AAGCAGCTAC ATTAGACTGT GAAACAACAA ATGGTGATTG TAAACCAGAA 
ATGTCAGAAA TTAAGCTTAA TTCTGATTCA GAGTATATTA AGCTCATGCA TAGGACATCT 
GCATGTTTGC CATCCTCCCA AAATGTAGAT TGTCAAATAA ATATCAATGG AGAATTGGAA 
AGACCACATT CACAGATGAA CAAAAACCAT GGTATTTTAC GAAGAAGTAT TTCATTGGGA 
GGAGCTTATC CAAATATTTC CTGTCTATCC AGCCTTAAGC ACAATTGTTC TAAAGGGGGA 
CCATCTCAGT TACTCATAAA GTTTGCATCT GGAAATGAAG GTAAAGTGGA TAATTTATCA 
AGAGACAGCA ACAGAGATTG CACAAATGAA CTGTCTAATT CTTGCAAGCC TGGCTGGGTT 
GTTTTGGACC AACCAGATGC TGCTTGCCAT TTGCAGCAAC AACAAGAACC TTCTCCTCTA 
CCTCCAGGGT GGGAAGAGAG GCAGGATATC CTTGGAAGGA CCTATTATGT AAACCATGAA 
TCTAGAAGAA CACAGTGGAA AAGACCAACC CCTCAGGACA ACCTAACAGA TGCTGAGAAT 
GGCAACATTC AACTGCAAGC ACAACGTGCA TTTACCACCA GGCGGCAGAT ATCCGAGGAA 
ACAGAAAGTG TTGACAACCG AGAGTCTTCC GAGAACTGGG AAATTATAAG AGAAGATGAA 
GCCACCATGT ATAGCAACCA GGCCTTCCCA TCACCTCCAC CGTCAAGTAA CTTGGATGTT 
CCAACTCATC TTGCAGAAGA ATTGAATGCC AGACTCACCA TTTTTGGAAA TTCAGCCGTG 
AGCCAGCCAG CATCGAGCTC AAATCATTCC AGCAGAAGAG GCAGCTTACA AGCCTATACT 
TTTGAGGAAC AACCTACACT TCCTGTGCTT TTGCCTACTT CATCTGGATT ACCACCAGGT 
TGGGAAGAAA AACAAGATGA AAGAGGAAGA TCATATTATG TAGATCACAA TTCCAGAACG 
ACTACTTGGA CAAAGCCCAC TGTACAGGCC ACAGTGGAGA CCAGTCAGCT GACCTCAAGC 
CAGAGTTCTG CAGGCCCTCA ATCACAAGCC TCCACCAGTG ATTCAGGCCA GCAGGTGACC 
CAGCCATCTG AAATTGAGCA AGGATTCCTT CCTAAAGGCT GGGAAGTCCG GCATGCACCA 
AATGGGAGGC CTTTCTTTAT TGACCACAAC ACTAAAACCA CCACCTGGGA AGATCCAAGA 
TTGAAAATTC CAGCCCATCT GAGAGGAAAG ACATCACTTG ATACTTCCAA TGATCTAGGG 
CCTTTACCTC CAGGATGGGA AGAGAGAACT CACACAGATG GAAGAATCTT CTACATAAAT 
CACAATATAA AAAGAACACA ATGGGAAGAT CCTCGGTTGG AGAATGTAGC AATAACTGGA 
CCAGCAGTGC CCTACTCCAG GGATTACAAA AGAAAGTATG AGTTCTTCCG AAGAAAGTTG 
AAGAAGCAGA ATGACATTCC AAACAAATTT GAAATGAAAC TTCGCCGAGC AACTGTTCTT 
GAAGACTCTT ACCGGAGAAT TATGGGTGTC AAGAGAGCAG ACTTCCTGAA GGCTCGACTG 
TGGATTGAGT TTGATGGTGA AAAGGGATTG GATTATGGAG GAGTTGCCAG AGAATGGTTC 
TTCCTGATCT CAAAGGAAAT GTTTAACCCT TATTATGGGT TGTTTGAATA TTCTGCTACG 
GACAATTATA CCCTACAGAT AAATCCAAAC TCTGGATTGT GTAACGAAGA TCACCTCTCT 
TACTTCAAGT TTATTGGTCG GGTAGCTGGA ATGGCAGTTT ATCATGGCAA ACTGTTGGAT 
GGTTTTTTCA TCCGCCCATT TTACAAGATG ATGCTTCACA AACCAATAAC CCTTCATGAT 
ATGGAATCTG TGGATAGTGA ATATTACAAT TCCCTAAGAT GGATTCTTGA AAATGACCCA 
ACAGAATTGG ACCTCAGGTT TATCATAGAT GAAGAACTTT TTGGACAGAC ACATCAACAT 
GAGCTGAAAA ATGGTGGATC AGAAATAGTT GTCACCAATA AGAACAAAAA GGAATATATT 
TATCTTGTAA TACAATGGCG ATTTGTAAAC CGAATCCAGA AGCAAATGGC TGCTTTTAAA 
GAGGGATTCT TTGAACTAAT ACCACAGGAT CTCATCAAAA TTTTTGATGA AAATGAACTA 
GAGCTTCTTA TGTGTGGACT GGGAGATGTT GATGTGAATG ACTGGAGGGA ACATACAAAG 
TATAAAAATG GCTACAGTGC AAATCATCAG GTTATACAGT GGTTTTGGAA GGCTGTTTTA 
ATGATGGATT CAGAAAAAAG AATAAGATTA CTTCAGTTTG TCACTGGCAC ATCTCGGGTG 
CCTATGAATG GATTTGCTGA ACTATACGGT TCAAATGGAC CACAGTCATT TACAGTTGAA 
CAGTGGGGTA CTCCTGAAAA GCTGCCAAGA GCTCATACCT GTTTTAATCG CCTGGACTTG 
CCACCTTATG AATCATTTGA AGAATTATGG GATAAACTTC AGATGGCAAT TGAAAACACC 
CAGGGCTTTG ATGGAGTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEDD4 ( NM_001284339.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271268.1 Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 4, mRNA.

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CloneID	OHu21763
Clone ID Related Accession (Same CDS sequence)	NM_001284339.1
Accession Version	NM_001284339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase NEDD4 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL832063.1, BC144285.1, BC136605.1 and AC009997.7. On Sep 28, 2013 this sequence version replaced XM_005254407.1. Transcript Variant: This variant (4) contains three alternate in-frame exons and uses an alternate in-frame splice site, compared to variant 2. The encoded isoform (4) is longer than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144285.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA CCCTTTGTCA 
GAAACCTCCG GAGATGACTT GGATAGCCAT GTTCACATGT GCTTCAAAAG ACCAACACGG 
ATTTCAACGT CTAACGTTGT TCAAATGAAG CTGACTCCCA GACAGACTGC ACTAGCTCCG 
TTAATAAAGG AAAACGTTCA GTCTCAAGAA AGATCATCTG TTCCCTCATC TGAAAATGTT 
AATAAAAAGA GCAGCTGTCT ACAGATTTCA CTACAGCCAA CAAGGTACAG TGGATATCTT 
CAGTCTAGCA ATGTCTTAGC TGATAGTGAT GATGCTTCGT TTACTTGTAT CTTGAAGGAT 
GGTATTTACA GTAGTGCTGT GGTCGATAAT GAATTGAATG CTGTGAATGA TGGTCACCTT 
GTAAGCAGTC CAGCCATTTG TAGTGGTAGC CTTAGTAACT TTTCAACCAG TGATAATGGG 
TCTTACAGCA GCAACGGTAG TGATTTTGGG TCATGTGCAA GTATCACAAG TGGAGGTTCA 
TATACTAACA GTGTCATCAG TGACAGTAGT AGTTATACTT TTCCACCAAG TGATGATACT 
TTTTTGGGTG GAAACTTACC TTCTGACAGC ACCTCCAATA GAAGTGTGCC AAACAGGAAT 
ACTACTCCTT GTGAAATTTT TTCAAGAAGT ACAAGTACAG ATCCTTTTGT CCAGGATGAC 
TTGGAACATG GATTAGAGAT TATGAAATTG CCAGTGAGCA GGAACACAAA AATTCCACTA 
AAACGTTACT CCTCCTTAGT CATTTTTCCT AGGAGTCCTT CAACTACCCG ACCGACTTCT 
CCAACAAGTC TGTGTACTCT TCTGAGCAAA GGATCCTATC AAACTTCACA CCAGTTTATT 
ATTTCTCCTA GTGAAATTGC ACATAATGAG GATGGCACTA GTGCTAAAGG ATTTCTTTCA 
ACAGCTGTCA ATGGACTTCG GTTATCTAAA ACAATTTGTA CCCCCGGAGA AGTAAGAGAC 
ATACGGCCGC TTCACAGGAA GGGCTCGTTA CAGAAGAAAA TTGTTCTTTC GAATAATACT 
CCCAGACAGA CTGTCTGTGA AAAGTCATCT GAAGGATATT CTTGTGTTTC AGTGCATTTC 
ACCCAACGAA AAGCAGCTAC ATTAGACTGT GAAACAACAA ATGGTGATTG TAAACCAGAA 
ATGTCAGAAA TTAAGCTTAA TTCTGATTCA GAGTATATTA AGCTCATGCA TAGGACATCT 
GCATGTTTGC CATCCTCCCA AAATGTAGAT TGTCAAATAA ATATCAATGG AGAATTGGAA 
AGACCACATT CACAGATGAA CAAAAACCAT GGTATTTTAC GAAGAAGTAT TTCATTGGGA 
GGAGCTTATC CAAATATTTC CTGTCTATCC AGCCTTAAGC ACAATTGTTC TAAAGGGGGA 
CCATCTCAGT TACTCATAAA GTTTGCATCT GGAAATGAAG GTAAAGTGGA TAATTTATCA 
AGAGACAGCA ACAGAGATTG CACAAATGAA CTGTCTAATT CTTGCAAGAC AAGAGATGAT 
TTCCTAGGTC AAGTGGATGT TCCACTTTAT CCATTACCGA CAGAAAATCC AAGATTGGAG 
AGACCATATA CATTTAAGGA TTTTGTTCTT CATCCAAGAA GTCACAAATC AAGAGTTAAA 
GGTTATCTGA GACTAAAAAT GACTTATTTA CCTAAAACCA GTGGCTCAGA AGATGATAAT 
GCAGAACAGG CTGAGGAATT AGAGCAACAA CAAGAACCTT CTCCTCTACC TCCAGGGTGG 
GAAGAGAGGC AGGATATCCT TGGAAGGACC TATTATGTAA ACCATGAATC TAGAAGAACA 
CAGTGGAAAA GACCAACCCC TCAGGACAAC CTAACAGATG CTGAGAATGG CAACATTCAA 
CTGCAAGCAC AACGTGCATT TACCACCAGG CGGCAGATAT CCGAGGAAAC AGAAAGTGTT 
GACAACCGAG AGTCTTCCGA GAACTGGGAA ATTATAAGAG AAGATGAAGC CACCATGTAT 
AGCAACCAGG CCTTCCCATC ACCTCCACCG TCAAGTAACT TGGATGTTCC AACTCATCTT 
GCAGAAGAAT TGAATGCCAG ACTCACCATT TTTGGAAATT CAGCCGTGAG CCAGCCAGCA 
TCGAGCTCAA ATCATTCCAG CAGAAGAGGC AGCTTACAAG CCTATACTTT TGAGGAACAA 
CCTACACTTC CTGTGCTTTT GCCTACTTCA TCTGGATTAC CACCAGGTTG GGAAGAAAAA 
CAAGATGAAA GAGGAAGATC ATATTATGTA GATCACAATT CCAGAACGAC TACTTGGACA 
AAGCCCACTG TACAGGCCAC AGTGGAGACC AGTCAGCTGA CCTCAAGCCA GAGTTCTGCA 
GGCCCTCAAT CACAAGCCTC CACCAGTGAT TCAGGCCAGC AGGTGACCCA GCCATCTGAA 
ATTGAGCAAG GATTCCTTCC TAAAGGCTGG GAAGTCCGGC ATGCACCAAA TGGGAGGCCT 
TTCTTTATTG ACCACAACAC TAAAACCACC ACCTGGGAAG ATCCAAGATT GAAAATTCCA 
GCCCATCTGA GAGGAAAGAC ATCACTTGAT ACTTCCAATG ATCTAGGGCC TTTACCTCCA 
GGATGGGAAG AGAGAACTCA CACAGATGGA AGAATCTTCT ACATAAATCA CAATATAAAA 
AGAACACAAT GGGAAGATCC TCGGTTGGAG AATGTAGCAA TAACTGGACC AGCAGTGCCC 
TACTCCAGGG ATTACAAAAG AAAGTATGAG TTCTTCCGAA GAAAGTTGAA GAAGCAGAAT 
GACATTCCAA ACAAATTTGA AATGAAACTT CGCCGAGCAA CTGTTCTTGA AGACTCTTAC 
CGGAGAATTA TGGGTGTCAA GAGAGCAGAC TTCCTGAAGG CTCGACTGTG GATTGAGTTT 
GATGGTGAAA AGGGATTGGA TTATGGAGGA GTTGCCAGAG AATGGTTCTT CCTGATCTCA 
AAGGAAATGT TTAACCCTTA TTATGGGTTG TTTGAATATT CTGCTACGGA CAATTATACC 
CTACAGATAA ATCCAAACTC TGGATTGTGT AACGAAGATC ACCTCTCTTA CTTCAAGTTT 
ATTGGTCGGG TAGCTGGAAT GGCAGTTTAT CATGGCAAAC TGTTGGATGG TTTTTTCATC 
CGCCCATTTT ACAAGATGAT GCTTCACAAA CCAATAACCC TTCATGATAT GGAATCTGTG 
GATAGTGAAT ATTACAATTC CCTAAGATGG ATTCTTGAAA ATGACCCAAC AGAATTGGAC 
CTCAGGTTTA TCATAGATGA AGAACTTTTT GGACAGACAC ATCAACATGA GCTGAAAAAT 
GGTGGATCAG AAATAGTTGT CACCAATAAG AACAAAAAGG AATATATTTA TCTTGTAATA 
CAATGGCGAT TTGTAAACCG AATCCAGAAG CAAATGGCTG CTTTTAAAGA GGGATTCTTT 
GAACTAATAC CACAGGATCT CATCAAAATT TTTGATGAAA ATGAACTAGA GCTTCTTATG 
TGTGGACTGG GAGATGTTGA TGTGAATGAC TGGAGGGAAC ATACAAAGTA TAAAAATGGC 
TACAGTGCAA ATCATCAGGT TATACAGTGG TTTTGGAAGG CTGTTTTAAT GATGGATTCA 
GAAAAAAGAA TAAGATTACT TCAGTTTGTC ACTGGCACAT CTCGGGTGCC TATGAATGGA 
TTTGCTGAAC TATACGGTTC AAATGGACCA CAGTCATTTA CAGTTGAACA GTGGGGTACT 
CCTGAAAAGC TGCCAAGAGC TCATACCTGT TTTAATCGCC TGGACTTGCC ACCTTATGAA 
TCATTTGAAG AATTATGGGA TAAACTTCAG ATGGCAATTG AAAACACCCA GGGCTTTGAT 
GGAGTTGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271268.1
CDS	301..4212
Translation	MAQSLRLHFAARRSNTYPLSETSGDDLDSHVHMCFKRPTRISTSNVVQMKLTPRQTALAPLIKENVQSQERSSVPSSENVNKKSSCLQISLQPTRYSGYLQSSNVLADSDDASFTCILKDGIYSSAVVDNELNAVNDGHLVSSPAICSGSLSNFSTSDNGSYSSNGSDFGSCASITSGGSYTNSVISDSSSYTFPPSDDTFLGGNLPSDSTSNRSVPNRNTTPCEIFSRSTSTDPFVQDDLEHGLEIMKLPVSRNTKIPLKRYSSLVIFPRSPSTTRPTSPTSLCTLLSKGSYQTSHQFIISPSEIAHNEDGTSAKGFLSTAVNGLRLSKTICTPGEVRDIRPLHRKGSLQKKIVLSNNTPRQTVCEKSSEGYSCVSVHFTQRKAATLDCETTNGDCKPEMSEIKLNSDSEYIKLMHRTSACLPSSQNVDCQININGELERPHSQMNKNHGILRRSISLGGAYPNISCLSSLKHNCSKGGPSQLLIKFASGNEGKVDNLSRDSNRDCTNELSNSCKTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	NM_001284339.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NEDD4 E3 ubiquitin protein ligase (NEDD4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284339.1

ORF Insert Sequence:

ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA CCCTTTGTCA 
GAAACCTCCG GAGATGACTT GGATAGCCAT GTTCACATGT GCTTCAAAAG ACCAACACGG 
ATTTCAACGT CTAACGTTGT TCAAATGAAG CTGACTCCCA GACAGACTGC ACTAGCTCCG 
TTAATAAAGG AAAACGTTCA GTCTCAAGAA AGATCATCTG TTCCCTCATC TGAAAATGTT 
AATAAAAAGA GCAGCTGTCT ACAGATTTCA CTACAGCCAA CAAGGTACAG TGGATATCTT 
CAGTCTAGCA ATGTCTTAGC TGATAGTGAT GATGCTTCGT TTACTTGTAT CTTGAAGGAT 
GGTATTTACA GTAGTGCTGT GGTCGATAAT GAATTGAATG CTGTGAATGA TGGTCACCTT 
GTAAGCAGTC CAGCCATTTG TAGTGGTAGC CTTAGTAACT TTTCAACCAG TGATAATGGG 
TCTTACAGCA GCAACGGTAG TGATTTTGGG TCATGTGCAA GTATCACAAG TGGAGGTTCA 
TATACTAACA GTGTCATCAG TGACAGTAGT AGTTATACTT TTCCACCAAG TGATGATACT 
TTTTTGGGTG GAAACTTACC TTCTGACAGC ACCTCCAATA GAAGTGTGCC AAACAGGAAT 
ACTACTCCTT GTGAAATTTT TTCAAGAAGT ACAAGTACAG ATCCTTTTGT CCAGGATGAC 
TTGGAACATG GATTAGAGAT TATGAAATTG CCAGTGAGCA GGAACACAAA AATTCCACTA 
AAACGTTACT CCTCCTTAGT CATTTTTCCT AGGAGTCCTT CAACTACCCG ACCGACTTCT 
CCAACAAGTC TGTGTACTCT TCTGAGCAAA GGATCCTATC AAACTTCACA CCAGTTTATT 
ATTTCTCCTA GTGAAATTGC ACATAATGAG GATGGCACTA GTGCTAAAGG ATTTCTTTCA 
ACAGCTGTCA ATGGACTTCG GTTATCTAAA ACAATTTGTA CCCCCGGAGA AGTAAGAGAC 
ATACGGCCGC TTCACAGGAA GGGCTCGTTA CAGAAGAAAA TTGTTCTTTC GAATAATACT 
CCCAGACAGA CTGTCTGTGA AAAGTCATCT GAAGGATATT CTTGTGTTTC AGTGCATTTC 
ACCCAACGAA AAGCAGCTAC ATTAGACTGT GAAACAACAA ATGGTGATTG TAAACCAGAA 
ATGTCAGAAA TTAAGCTTAA TTCTGATTCA GAGTATATTA AGCTCATGCA TAGGACATCT 
GCATGTTTGC CATCCTCCCA AAATGTAGAT TGTCAAATAA ATATCAATGG AGAATTGGAA 
AGACCACATT CACAGATGAA CAAAAACCAT GGTATTTTAC GAAGAAGTAT TTCATTGGGA 
GGAGCTTATC CAAATATTTC CTGTCTATCC AGCCTTAAGC ACAATTGTTC TAAAGGGGGA 
CCATCTCAGT TACTCATAAA GTTTGCATCT GGAAATGAAG GTAAAGTGGA TAATTTATCA 
AGAGACAGCA ACAGAGATTG CACAAATGAA CTGTCTAATT CTTGCAAGAC AAGAGATGAT 
TTCCTAGGTC AAGTGGATGT TCCACTTTAT CCATTACCGA CAGAAAATCC AAGATTGGAG 
AGACCATATA CATTTAAGGA TTTTGTTCTT CATCCAAGAA GTCACAAATC AAGAGTTAAA 
GGTTATCTGA GACTAAAAAT GACTTATTTA CCTAAAACCA GTGGCTCAGA AGATGATAAT 
GCAGAACAGG CTGAGGAATT AGAGCAACAA CAAGAACCTT CTCCTCTACC TCCAGGGTGG 
GAAGAGAGGC AGGATATCCT TGGAAGGACC TATTATGTAA ACCATGAATC TAGAAGAACA 
CAGTGGAAAA GACCAACCCC TCAGGACAAC CTAACAGATG CTGAGAATGG CAACATTCAA 
CTGCAAGCAC AACGTGCATT TACCACCAGG CGGCAGATAT CCGAGGAAAC AGAAAGTGTT 
GACAACCGAG AGTCTTCCGA GAACTGGGAA ATTATAAGAG AAGATGAAGC CACCATGTAT 
AGCAACCAGG CCTTCCCATC ACCTCCACCG TCAAGTAACT TGGATGTTCC AACTCATCTT 
GCAGAAGAAT TGAATGCCAG ACTCACCATT TTTGGAAATT CAGCCGTGAG CCAGCCAGCA 
TCGAGCTCAA ATCATTCCAG CAGAAGAGGC AGCTTACAAG CCTATACTTT TGAGGAACAA 
CCTACACTTC CTGTGCTTTT GCCTACTTCA TCTGGATTAC CACCAGGTTG GGAAGAAAAA 
CAAGATGAAA GAGGAAGATC ATATTATGTA GATCACAATT CCAGAACGAC TACTTGGACA 
AAGCCCACTG TACAGGCCAC AGTGGAGACC AGTCAGCTGA CCTCAAGCCA GAGTTCTGCA 
GGCCCTCAAT CACAAGCCTC CACCAGTGAT TCAGGCCAGC AGGTGACCCA GCCATCTGAA 
ATTGAGCAAG GATTCCTTCC TAAAGGCTGG GAAGTCCGGC ATGCACCAAA TGGGAGGCCT 
TTCTTTATTG ACCACAACAC TAAAACCACC ACCTGGGAAG ATCCAAGATT GAAAATTCCA 
GCCCATCTGA GAGGAAAGAC ATCACTTGAT ACTTCCAATG ATCTAGGGCC TTTACCTCCA 
GGATGGGAAG AGAGAACTCA CACAGATGGA AGAATCTTCT ACATAAATCA CAATATAAAA 
AGAACACAAT GGGAAGATCC TCGGTTGGAG AATGTAGCAA TAACTGGACC AGCAGTGCCC 
TACTCCAGGG ATTACAAAAG AAAGTATGAG TTCTTCCGAA GAAAGTTGAA GAAGCAGAAT 
GACATTCCAA ACAAATTTGA AATGAAACTT CGCCGAGCAA CTGTTCTTGA AGACTCTTAC 
CGGAGAATTA TGGGTGTCAA GAGAGCAGAC TTCCTGAAGG CTCGACTGTG GATTGAGTTT 
GATGGTGAAA AGGGATTGGA TTATGGAGGA GTTGCCAGAG AATGGTTCTT CCTGATCTCA 
AAGGAAATGT TTAACCCTTA TTATGGGTTG TTTGAATATT CTGCTACGGA CAATTATACC 
CTACAGATAA ATCCAAACTC TGGATTGTGT AACGAAGATC ACCTCTCTTA CTTCAAGTTT 
ATTGGTCGGG TAGCTGGAAT GGCAGTTTAT CATGGCAAAC TGTTGGATGG TTTTTTCATC 
CGCCCATTTT ACAAGATGAT GCTTCACAAA CCAATAACCC TTCATGATAT GGAATCTGTG 
GATAGTGAAT ATTACAATTC CCTAAGATGG ATTCTTGAAA ATGACCCAAC AGAATTGGAC 
CTCAGGTTTA TCATAGATGA AGAACTTTTT GGACAGACAC ATCAACATGA GCTGAAAAAT 
GGTGGATCAG AAATAGTTGT CACCAATAAG AACAAAAAGG AATATATTTA TCTTGTAATA 
CAATGGCGAT TTGTAAACCG AATCCAGAAG CAAATGGCTG CTTTTAAAGA GGGATTCTTT 
GAACTAATAC CACAGGATCT CATCAAAATT TTTGATGAAA ATGAACTAGA GCTTCTTATG 
TGTGGACTGG GAGATGTTGA TGTGAATGAC TGGAGGGAAC ATACAAAGTA TAAAAATGGC 
TACAGTGCAA ATCATCAGGT TATACAGTGG TTTTGGAAGG CTGTTTTAAT GATGGATTCA 
GAAAAAAGAA TAAGATTACT TCAGTTTGTC ACTGGCACAT CTCGGGTGCC TATGAATGGA 
TTTGCTGAAC TATACGGTTC AAATGGACCA CAGTCATTTA CAGTTGAACA GTGGGGTACT 
CCTGAAAAGC TGCCAAGAGC TCATACCTGT TTTAATCGCC TGGACTTGCC ACCTTATGAA 
TCATTTGAAG AATTATGGGA TAAACTTCAG ATGGCAATTG AAAACACCCA GGGCTTTGAT 
GGAGTTGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEDD4
Entrez Gene ID	4734
Full Name	neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
Synonyms	NEDD4-1,RPF1
General protein information	Preferred Names neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase NEDD4 HECT-type E3 ubiquitin transferase NEDD4 cell proliferation-inducing gene 53 protein receptor-potentiating factor 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q21.3
Summary	This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016].
Disorder MIM:	602278

Gallus gallus (chicken)	NEDD4	XP_004943843.1
Homo sapiens (human)	NEDD4	NP_001271267.1
Bos taurus (cattle)	NEDD4	XP_002690936.1
	NEDD4	XP_001088005.2
Canis lupus familiaris (dog)	NEDD4	XP_005638494.1
Xenopus tropicalis (tropical clawed frog)	nedd4	XP_004912758.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEDD4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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