DRG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DRG1
Entrez Gene ID	4733
Full Name	developmentally regulated GTP binding protein 1
Synonyms	NEDD3
General protein information	Preferred Names developmentally regulated GTP binding protein 1 Names developmentally-regulated GTP-binding protein 1 DRG-1 NEDD-3 neural precursor cell expressed developmentally down-regulated protein 3 neural precursor cell expressed, developmentally down-regulated 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q12.2
Disorder MIM:	603952

mRNA and Protein(s)

mRNA	Protein	Name
NM_004147.3	NP_004138.1	developmentally-regulated GTP-binding protein 1
NM_004147.4	NP_004138.1	developmentally-regulated GTP-binding protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DRG1	XP_534738.2
Mus musculus (house mouse)	Drg1	NP_031905.1
Drosophila melanogaster (fruit fly)	128up	NP_536733.1
Eremothecium gossypii	AGOS_ADR372C	NP_984468.1
	DRG1	XP_001110881.1
Bos taurus (cattle)	DRG1	NP_001029863.1
Rattus norvegicus (Norway rat)	Drg1	NP_001009685.1
Kluyveromyces lactis	KLLA0F23287g	XP_456117.1
Arabidopsis thaliana (thale cress)	AT4G39520	NP_195662.1
Xenopus tropicalis (tropical clawed frog)	drg1	NP_001016294.1
Homo sapiens (human)	DRG1	NP_004138.1
Pan troglodytes (chimpanzee)	DRG1	XP_515085.2
Danio rerio (zebrafish)	drg1	NP_956332.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010624	XP_310117.3
Magnaporthe oryzae (rice blast fungus)	MGG_05730	XP_003710640.1
Oryza sativa (rice)	Os07g0627800	NP_001060347.1
Gallus gallus (chicken)	DRG1	NP_001027533.1
Saccharomyces cerevisiae (baker's yeast)	RBG1	NP_009364.1
Neurospora crassa	NCU00389	XP_957275.2
Caenorhabditis elegans (roundworm)	T28D6.6	NP_001255126.1
Schizosaccharomyces pombe (fission yeast)	SPAC9.07c	NP_593350.1

Gene Ontology
Bibliography

Related articles in PubMed

Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics.
Schellhaus AK, Moreno-Andr?s D, Chugh M, Yokoyama H, Moschopoulou A, De S, Bono F, Hipp K, Sch?ffer E, Antonin W
Scientific reports7(1)9996(2017 Aug)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

DRG1 is a potential oncogene in lung adenocarcinoma and promotes tumor progression via spindle checkpoint signaling regulation.
Lu L, Lv Y, Dong J, Hu S, Peng R
Oncotarget7(45)72795-72806(2016 Nov)

Identification of DRG-1 As a Melanoma-Associated Antigen Recognized by CD4+ Th1 Cells.
Kiniwa Y, Li J, Wang M, Sun C, Lee JE, Wang RF, Wang HY
PloS one10(5)e0124094(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DRG-1 plays an important role in melanoma cell growth and transformation, indicating that DRG1 may represent a novel target for CD4(+) T cell-mediated immunotherapy in melanoma.
Title: Identification of DRG-1 As a Melanoma-Associated Antigen Recognized by CD4+ Th1 Cells.

Lerepo4 action leaves Drg1 affinity for nucleotides unaffected, feasibly favoring a switch I reorientation, mainly via the TGS domain.
Title: Human Drg1 is a potassium-dependent GTPase enhanced by Lerepo4.

Observational study of gene-disease association. (HuGE Navigator)
Title: A common variant in DRD3 receptor is associated with autism spectrum disorder.

The tumor metastasis suppressor gene Drg-1 suppresses metastasis of prostate tumor cells by inhibiting the invasive ability of the cells via down-regulation of the expression of the ATF3 gene.
Title: The tumor metastasis suppressor gene Drg-1 down-regulates the expression of activating transcription factor 3 in prostate cancer.

our results strongly suggest functional involvement of the Drg-1 gene in suppressing the metastatic advancement of human breast cancer.
Title: Role of the putative tumor metastasis suppressor gene Drg-1 in breast cancer progression.

The following DRG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DRG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30834	NM_004147.4 Latest version!	Homo sapiens developmentally regulated GTP binding protein 1 (DRG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu30834	NM_004147.3	Homo sapiens developmentally regulated GTP binding protein 1 (DRG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DRG1 ( NM_004147.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004138.1 Homo sapiens developmentally regulated GTP binding protein 1 (DRG1), mRNA.

Price & Availability↑

CloneID	OHu30834
Clone ID Related Accession (Same CDS sequence)	NM_004147.3 , NM_004147.4
Accession Version	NM_004147.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	developmentally-regulated GTP-binding protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU584654.1 and BC019285.1. On or before Aug 3, 2011 this sequence version replaced XM_003403741.1, NM_004147.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.57405.1, ERR279870.2795.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCAGCA CCTTAGCTAA GATCGCGGAG ATAGAAGCAG AGATGGCTCG GACTCAAAAG 
AACAAGGCCA CAGCACACCA CTTAGGGCTG CTTAAGGCTC GTCTTGCTAA GCTTCGTCGA 
GAACTCATTA CTCCAAAGGG TGGTGGTGGT GGAGGTCCAG GAGAAGGTTT TGATGTGGCC 
AAGACAGGTG ATGCTCGAAT TGGATTTGTT GGTTTTCCAT CTGTGGGGAA GTCAACACTG 
CTTAGTAACC TGGCAGGGGT ATATTCTGAG GTGGCAGCCT ATGAATTCAC TACTCTGACC 
ACTGTGCCTG GTGTCATCAG ATACAAAGGT GCCAAGATCC AGCTCCTGGA TCTCCCAGGT 
ATCATTGAAG GTGCCAAGGA TGGGAAAGGT AGAGGTCGTC AAGTCATTGC AGTGGCCCGA 
ACCTGTAACT TGATCTTGAT TGTTCTGGAT GTCCTGAAAC CTTTGGGACA TAAGAAGATA 
ATTGAAAATG AGCTGGAAGG CTTTGGCATT CGCTTGAACA GCAAACCCCC CAACATTGGC 
TTTAAGAAGA AGGACAAGGG AGGCATTAAT CTCACAGCCA CTTGCCCCCA GAGTGAGCTG 
GATGCTGAAA CTGTGAAGAG CATTCTGGCT GAATACAAGA TTCATAATGC CGATGTGACT 
CTACGTAGTG ATGCTACAGC TGATGACCTC ATTGATGTGG TGGAAGGAAA CAGAGTTTAT 
ATCCCCTGTA TCTATGTGTT AAATAAGATT GACCAAATCT CCATTGAGGA ATTGGATATC 
ATCTATAAGG TGCCTCACTG TGTACCCATC TCTGCCCATC ACCGCTGGAA TTTTGATGAC 
CTATTGGAAA AGATCTGGGA CTATCTGAAA CTAGTGAGAA TTTACACCAA ACCCAAAGGC 
CAGTTACCAG ATTACACATC CCCAGTGGTG CTTCCTTACT CCAGGACCAC AGTGGAGGAT 
TTCTGCATGA AGATTCACAA AAATCTTATC AAAGAATTTA AATATGCTCT GGTCTGGGGT 
CTCTCTGTGA AACACAATCC TCAGAAAGTG GGTAAAGACC ATACGTTGGA GGATGAGGAT 
GTCATTCAAA TTGTGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004138.1
CDS	132..1235
Translation	MSSTLAKIAEIEAEMARTQKNKATAHHLGLLKARLAKLRRELITPKGGGGGGPGEGFDVAKTGDARIGFVGFPSVGKSTLLSNLAGVYSEVAAYEFTTLTTVPGVIRYKGAKIQLLDLPGIIEGAKDGKGRGRQVIAVARTCNLILIVLDVLKPLGHKKIIENELEGFGIRLNSKPPNIGFKKKDKGGINLTATCPQSELDAETVKSILAEYKIHNADVTLRSDATADDLIDVVEGNRVYIPCIYVLNKIDQISIEELDIIYKVPHCVPISAHHRWNFDDLLEKIWDYLKLVRIYTKPKGQLPDYTSPVVLPYSRTTVEDFCMKIHKNLIKEFKYALVWGLSVKHNPQKVGKDHTLEDEDVIQIVKK

Target ORF information:

RefSeq Version	NM_004147.3
Organism	Homo sapiens(human)
Definition	Homo sapiens developmentally regulated GTP binding protein 1 (DRG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004147.3

ORF Insert Sequence:

ATGAGCAGCA CCTTAGCTAA GATCGCGGAG ATAGAAGCAG AGATGGCTCG GACTCAAAAG 
AACAAGGCCA CAGCACACCA CTTAGGGCTG CTTAAGGCTC GTCTTGCTAA GCTTCGTCGA 
GAACTCATTA CTCCAAAGGG TGGTGGTGGT GGAGGTCCAG GAGAAGGTTT TGATGTGGCC 
AAGACAGGTG ATGCTCGAAT TGGATTTGTT GGTTTTCCAT CTGTGGGGAA GTCAACACTG 
CTTAGTAACC TGGCAGGGGT ATATTCTGAG GTGGCAGCCT ATGAATTCAC TACTCTGACC 
ACTGTGCCTG GTGTCATCAG ATACAAAGGT GCCAAGATCC AGCTCCTGGA TCTCCCAGGT 
ATCATTGAAG GTGCCAAGGA TGGGAAAGGT AGAGGTCGTC AAGTCATTGC AGTGGCCCGA 
ACCTGTAACT TGATCTTGAT TGTTCTGGAT GTCCTGAAAC CTTTGGGACA TAAGAAGATA 
ATTGAAAATG AGCTGGAAGG CTTTGGCATT CGCTTGAACA GCAAACCCCC CAACATTGGC 
TTTAAGAAGA AGGACAAGGG AGGCATTAAT CTCACAGCCA CTTGCCCCCA GAGTGAGCTG 
GATGCTGAAA CTGTGAAGAG CATTCTGGCT GAATACAAGA TTCATAATGC CGATGTGACT 
CTACGTAGTG ATGCTACAGC TGATGACCTC ATTGATGTGG TGGAAGGAAA CAGAGTTTAT 
ATCCCCTGTA TCTATGTGTT AAATAAGATT GACCAAATCT CCATTGAGGA ATTGGATATC 
ATCTATAAGG TGCCTCACTG TGTACCCATC TCTGCCCATC ACCGCTGGAA TTTTGATGAC 
CTATTGGAAA AGATCTGGGA CTATCTGAAA CTAGTGAGAA TTTACACCAA ACCCAAAGGC 
CAGTTACCAG ATTACACATC CCCAGTGGTG CTTCCTTACT CCAGGACCAC AGTGGAGGAT 
TTCTGCATGA AGATTCACAA AAATCTTATC AAAGAATTTA AATATGCTCT GGTCTGGGGT 
CTCTCTGTGA AACACAATCC TCAGAAAGTG GGTAAAGACC ATACGTTGGA GGATGAGGAT 
GTCATTCAAA TTGTGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DRG1 ( NM_004147.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004138.1 Homo sapiens developmentally regulated GTP binding protein 1 (DRG1), mRNA.

Price & Availability↑

CloneID	OHu30834
Clone ID Related Accession (Same CDS sequence)	NM_004147.3 , NM_004147.4
Accession Version	NM_004147.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	developmentally-regulated GTP-binding protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU584654.1, BC019285.1 and AL096701.14. On May 17, 2019 this sequence version replaced NM_004147.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.305923.1, AF078103.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000331457.9/ ENSP00000329715.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCAGCA CCTTAGCTAA GATCGCGGAG ATAGAAGCAG AGATGGCTCG GACTCAAAAG 
AACAAGGCCA CAGCACACCA CTTAGGGCTG CTTAAGGCTC GTCTTGCTAA GCTTCGTCGA 
GAACTCATTA CTCCAAAGGG TGGTGGTGGT GGAGGTCCAG GAGAAGGTTT TGATGTGGCC 
AAGACAGGTG ATGCTCGAAT TGGATTTGTT GGTTTTCCAT CTGTGGGGAA GTCAACACTG 
CTTAGTAACC TGGCAGGGGT ATATTCTGAG GTGGCAGCCT ATGAATTCAC TACTCTGACC 
ACTGTGCCTG GTGTCATCAG ATACAAAGGT GCCAAGATCC AGCTCCTGGA TCTCCCAGGT 
ATCATTGAAG GTGCCAAGGA TGGGAAAGGT AGAGGTCGTC AAGTCATTGC AGTGGCCCGA 
ACCTGTAACT TGATCTTGAT TGTTCTGGAT GTCCTGAAAC CTTTGGGACA TAAGAAGATA 
ATTGAAAATG AGCTGGAAGG CTTTGGCATT CGCTTGAACA GCAAACCCCC CAACATTGGC 
TTTAAGAAGA AGGACAAGGG AGGCATTAAT CTCACAGCCA CTTGCCCCCA GAGTGAGCTG 
GATGCTGAAA CTGTGAAGAG CATTCTGGCT GAATACAAGA TTCATAATGC CGATGTGACT 
CTACGTAGTG ATGCTACAGC TGATGACCTC ATTGATGTGG TGGAAGGAAA CAGAGTTTAT 
ATCCCCTGTA TCTATGTGTT AAATAAGATT GACCAAATCT CCATTGAGGA ATTGGATATC 
ATCTATAAGG TGCCTCACTG TGTACCCATC TCTGCCCATC ACCGCTGGAA TTTTGATGAC 
CTATTGGAAA AGATCTGGGA CTATCTGAAA CTAGTGAGAA TTTACACCAA ACCCAAAGGC 
CAGTTACCAG ATTACACATC CCCAGTGGTG CTTCCTTACT CCAGGACCAC AGTGGAGGAT 
TTCTGCATGA AGATTCACAA AAATCTTATC AAAGAATTTA AATATGCTCT GGTCTGGGGT 
CTCTCTGTGA AACACAATCC TCAGAAAGTG GGTAAAGACC ATACGTTGGA GGATGAGGAT 
GTCATTCAAA TTGTGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004138.1
CDS	81..1184
Translation	MSSTLAKIAEIEAEMARTQKNKATAHHLGLLKARLAKLRRELITPKGGGGGGPGEGFDVAKTGDARIGFVGFPSVGKSTLLSNLAGVYSEVAAYEFTTLTTVPGVIRYKGAKIQLLDLPGIIEGAKDGKGRGRQVIAVARTCNLILIVLDVLKPLGHKKIIENELEGFGIRLNSKPPNIGFKKKDKGGINLTATCPQSELDAETVKSILAEYKIHNADVTLRSDATADDLIDVVEGNRVYIPCIYVLNKIDQISIEELDIIYKVPHCVPISAHHRWNFDDLLEKIWDYLKLVRIYTKPKGQLPDYTSPVVLPYSRTTVEDFCMKIHKNLIKEFKYALVWGLSVKHNPQKVGKDHTLEDEDVIQIVKK

Target ORF information:

RefSeq Version	NM_004147.4
Organism	Homo sapiens(human)
Definition	Homo sapiens developmentally regulated GTP binding protein 1 (DRG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004147.4

ORF Insert Sequence:

ATGAGCAGCA CCTTAGCTAA GATCGCGGAG ATAGAAGCAG AGATGGCTCG GACTCAAAAG 
AACAAGGCCA CAGCACACCA CTTAGGGCTG CTTAAGGCTC GTCTTGCTAA GCTTCGTCGA 
GAACTCATTA CTCCAAAGGG TGGTGGTGGT GGAGGTCCAG GAGAAGGTTT TGATGTGGCC 
AAGACAGGTG ATGCTCGAAT TGGATTTGTT GGTTTTCCAT CTGTGGGGAA GTCAACACTG 
CTTAGTAACC TGGCAGGGGT ATATTCTGAG GTGGCAGCCT ATGAATTCAC TACTCTGACC 
ACTGTGCCTG GTGTCATCAG ATACAAAGGT GCCAAGATCC AGCTCCTGGA TCTCCCAGGT 
ATCATTGAAG GTGCCAAGGA TGGGAAAGGT AGAGGTCGTC AAGTCATTGC AGTGGCCCGA 
ACCTGTAACT TGATCTTGAT TGTTCTGGAT GTCCTGAAAC CTTTGGGACA TAAGAAGATA 
ATTGAAAATG AGCTGGAAGG CTTTGGCATT CGCTTGAACA GCAAACCCCC CAACATTGGC 
TTTAAGAAGA AGGACAAGGG AGGCATTAAT CTCACAGCCA CTTGCCCCCA GAGTGAGCTG 
GATGCTGAAA CTGTGAAGAG CATTCTGGCT GAATACAAGA TTCATAATGC CGATGTGACT 
CTACGTAGTG ATGCTACAGC TGATGACCTC ATTGATGTGG TGGAAGGAAA CAGAGTTTAT 
ATCCCCTGTA TCTATGTGTT AAATAAGATT GACCAAATCT CCATTGAGGA ATTGGATATC 
ATCTATAAGG TGCCTCACTG TGTACCCATC TCTGCCCATC ACCGCTGGAA TTTTGATGAC 
CTATTGGAAA AGATCTGGGA CTATCTGAAA CTAGTGAGAA TTTACACCAA ACCCAAAGGC 
CAGTTACCAG ATTACACATC CCCAGTGGTG CTTCCTTACT CCAGGACCAC AGTGGAGGAT 
TTCTGCATGA AGATTCACAA AAATCTTATC AAAGAATTTA AATATGCTCT GGTCTGGGGT 
CTCTCTGTGA AACACAATCC TCAGAAAGTG GGTAAAGACC ATACGTTGGA GGATGAGGAT 
GTCATTCAAA TTGTGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics.
Scientific reports7(1)9996(2017 Aug)
Schellhaus AK,Moreno-Andr?s D,Chugh M,Yokoyama H,Moschopoulou A,De S,Bono F,Hipp K,Sch?ffer E,Antonin W

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

DRG1 is a potential oncogene in lung adenocarcinoma and promotes tumor progression via spindle checkpoint signaling regulation.
Oncotarget7(45)72795-72806(2016 Nov)
Lu L,Lv Y,Dong J,Hu S,Peng R

Identification of DRG-1 As a Melanoma-Associated Antigen Recognized by CD4+ Th1 Cells.
PloS one10(5)e0124094(2015)
Kiniwa Y,Li J,Wang M,Sun C,Lee JE,Wang RF,Wang HY

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DRG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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