MYH1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH1
Entrez Gene ID	4619
Full Name	myosin heavy chain 1
Synonyms	HEL71,MYHSA1,MYHa,MyHC-2X/D,MyHC-2x
General protein information	Preferred Names myosin heavy chain 1 Names myosin-1 epididymis luminal protein 71 myHC-IIx/d myosin heavy chain 2x myosin heavy chain IIx/d myosin heavy chain, skeletal muscle, adult 1 myosin, heavy chain 1, skeletal muscle, adult myosin, heavy polypeptide 1, skeletal muscle, adult
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.1
Summary	Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008].
Disorder MIM:	160730

mRNA and Protein(s)

mRNA	Protein	Name
XM_017024675.1	XP_016880164.1	myosin-1 isoform X1
NM_005963.3	NP_005954.3	myosin-1
NM_005963.4	NP_005954.3	myosin-1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYH1	NP_005954.3
Xenopus tropicalis (tropical clawed frog)	myh8	XP_004916771.1
Xenopus tropicalis (tropical clawed frog)	LOC100495802	XP_002935255.1
Bos taurus (cattle)	MYH1	NP_776542.1
Xenopus tropicalis (tropical clawed frog)	myh4	NP_001001244.1
Xenopus tropicalis (tropical clawed frog)	myh2	NP_001006805.1
Xenopus tropicalis (tropical clawed frog)	LOC100495968	XP_002935256.2
Pan troglodytes (chimpanzee)	MYH1	XP_003953177.1
Canis lupus familiaris (dog)	MYH1	NP_001107189.1
Mus musculus (house mouse)	Myh1	NP_109604.1
Rattus norvegicus (Norway rat)	Myh1	NP_001128630.1
Xenopus tropicalis (tropical clawed frog)	LOC100495638	XP_002935254.1

Gene Ontology
Bibliography

Related articles in PubMed

Transition of myosin heavy chain isoforms in human laryngeal abductors following denervation.
Qiu X, Chen D, Li M, Gao Y, Liu F, Zheng H, Chen S
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery272(10)2915-23(2015 Oct)

Molecular determinants of force production in human skeletal muscle fibers: effects of myosin isoform expression and cross-sectional area.
Miller MS, Bedrin NG, Ades PA, Palmer BM, Toth MJ
American journal of physiology. Cell physiology308(6)C473-84(2015 Mar)

Wearing of complete dentures reduces slow fibre and enhances hybrid fibre fraction in masseter muscle.
Cvetko E, Karen P, Er?en I
Journal of oral rehabilitation39(8)608-14(2012 Aug)

Myosin heavy chain composition of the human genioglossus muscle.
Daugherty M, Luo Q, Sokoloff AJ
Journal of speech, language, and hearing research : JSLHR55(2)609-25(2012 Apr)

The expression of myosin heavy chain (MHC) genes in human skeletal muscle is related to metabolic characteristics involved in the pathogenesis of type 2 diabetes.
Olsson AH, R?nn T, Elgzyri T, Hansson O, Eriksson KF, Groop L, Vaag A, Poulsen P, Ling C
Molecular genetics and metabolism103(3)275-81(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Transcriptional levels of MHC-I, MHC-IIa, and MHC-IIb in denervation groups were significantly down-regulated compared with controls
Title: Transition of myosin heavy chain isoforms in human laryngeal abductors following denervation.

Myosin isoforms impact single-fiber force generation and may lead to alterations in whole skeletal muscle performance.
Title: Molecular determinants of force production in human skeletal muscle fibers: effects of myosin isoform expression and cross-sectional area.

adaptive response to reduced masticatory load was lower numerical and area proportion of MyHC-1 expressing fibres and higher numerical proportion of hybrid fibres in edentulous compared with dentate subjects
Title: Wearing of complete dentures reduces slow fibre and enhances hybrid fibre fraction in masseter muscle.

The human genioglossus muscle is composed of conventional myosin heavy chain isoforms and 3 primary myosin heavy chain phenotypes.
Title: Myosin heavy chain composition of the human genioglossus muscle.

the expression levels of the MHC genes are associated with age and both PGC-1alpha and PGC-1beta and indicate that the MHC genes may to some extent be used to determine fibre-type composition in human skeletal muscle.
Title: The expression of myosin heavy chain (MHC) genes in human skeletal muscle is related to metabolic characteristics involved in the pathogenesis of type 2 diabetes.

The following MYH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05264	NM_005963.4 Latest version!	Homo sapiens myosin heavy chain 1 (MYH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu05264	XM_017024675.1 Latest version!	Homo sapiens myosin heavy chain 1 (MYH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu05264	NM_005963.3	Homo sapiens myosin heavy chain 1 (MYH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH1 ( NM_005963.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005954.3 Homo sapiens myosin heavy chain 1 (MYH1), mRNA.

Price & Availability↑

CloneID	OHu05264
Clone ID Related Accession (Same CDS sequence)	XM_017024675.1 , NM_005963.3 , NM_005963.4
Accession Version	NM_005963.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	myosin-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA826478.1, BC114545.1, AF111785.1 and AJ573764.1. On Oct 4, 2006 this sequence version replaced NM_005963.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF111785.1, EU794637.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTCCG ACTCTGAGAT GGCCATTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAGGGAGC GAATTGAAGC CCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTGGACCCTA AGGAGTCCTT TGTGAAAGCA ACAGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCTAAGA CCGAAGCTGG AGCTACTGTA ACAGTGAAAG ATGACCAAGT CTTCCCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAC ATGGCCATGA TGACTCATCT ACACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGCTAC GCAGCCTGGA TGATCTACAC CTACTCAGGC 
TTGTTCTGTG TCACTGTCAA CCCCTACAAG TGGTTGCCAG TGTATAATGC AGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGCGCAG GGAAGACTGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGGG AGAAGAAGAA GGAAGAAGTT ACTTCTGGCA AAATGCAGGG GACTCTGGAA 
GATCAAATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTCGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTTAC TTTCCAGCTA 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGATCTA 
ATTGAAATGC TCCTGATCAC CACCAACCCA TACGATTATG CCTTCGTCAG TCAAGGGGAG 
ATCACAGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CTACAGATAG TGCCATTGAA 
ATTCTGGGCT TTACTTCAGA TGAAAGAGTG TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAGGC AGCCTATCTC CAAAATCTGA ACTCTGCAGA TCTGCTCAAA 
GCCCTCTGCT ACCCTAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG TCAAACTGTG 
CAGCAGGTGT ACAATGCAGT GGGTGCTCTG GCCAAAGCTG TCTACGATAA GATGTTCTTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATT 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAG TGGACGTTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAACATCTT 
GGAAAATCCA ATAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGCCTGA GGCCCACTTC 
TCTTTGATTC ACTATGCTGG CACCGTGGAC TACAACATTG CCGGCTGGCT TGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTG 
GCTCTCCTCT TTGTTGGGGC AACGGGAGCG GAAGCAGAGG CTGGCGGTGG AAAGAAAGGT 
GGTAAGAAGA AGGGTTCTTC TTTCCAGACT GTGTCTGCTC TCTTCAGGGA GAATTTGAAT 
AAGCTGATGA CCAACTTGAG GAGCACTCAC CCCCACTTTG TGCGGTGCAT CATCCCCAAT 
GAAACTAAAA CTCCTGGTGC CATGGAGCAT GAGCTTGTCC TGCATCAGCT GAGGTGTAAC 
GGTGTGCTGG AAGGCATCCG CATCTGCAGG AAAGGCTTCC CAAGCAGAAT CCTTTATGCA 
GACTTCAAAC AGAGATACAA GGTGTTAAAT GCAAGTGCTA TCCCTGAAGG ACAATTCATC 
GATAGCAAGA AGGCTTCAGA GAAGCTCCTG GGGTCCATTG ACATTGACCA CACCCAGTAT 
AAATTTGGTC ACACCAAGGT CTTTTTCAAA GCTGGTCTTC TGGGGCTCCT AGAGGAGATG 
CGAGATGAGA AGCTGGCCCA GCTGATTACC CGAACCCAGG CCATGTGCAG AGGGTTCTTG 
GCAAGAGTGG AGTACCAGAA AATGGTGGAA AGAAGAGAGT CCATCTTCTG CATCCAGTAC 
AATGTCCGTG CCTTCATGAA TGTGAAGCAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAACCCCTCC TCAAAAGTGC AGAGACAGAG AAGGAGATGG CCAACATGAA GGAAGAATTT 
GAGAAAACCA AAGAAGAGCT GGCTAAGACC GAGGCAAAAA GGAAAGAGCT GGAAGAAAAA 
ATGGTTACTC TGATGCAAGA AAAAAATGAC TTGCAACTCC AGGTTCAAGC TGAAGCTGAC 
AGCTTGGCTG ATGCAGAGGA AAGGTGTGAC CAGCTAATCA AAACCAAAAT CCAGCTAGAA 
GCCAAAATCA AAGAGGTGAC TGAGAGAGCT GAGGATGAGG AAGAGATCAA TGCTGAGCTG 
ACAGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAAC TCAAGAAAGA CATTGATGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAACATG CCACAGAAAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC GGGTCTGGAT GAAACCATTG CTAAGCTGAC CAAGGAGAAG 
AAGGCTCTCC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGGCAGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC TAAAATCAAA CTTGAACAAC AAGTGGATGA TCTTGAAGGA 
TCTTTGGAAC AAGAAAAGAA AATCCGGATG GATCTAGAAA GAGCAAAGAG AAAACTAGAG 
GGAGACCTAA AATTGGCTCA AGAATCCACA ATGGATATAG AAAATGACAA ACAACAACTT 
GATGAAAAGC TTAAAAAGAA AGAGTTTGAA ATGAGCGGTC TGCAAAGCAA GATTGAAGAT 
GAACAAGCCC TTGGTATGCA GCTGCAGAAG AAAATCAAGG AGTTACAAGC CCGCATTGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCCGGG CCAAAGCAGA GAAGCAGCGC 
TCTGATCTCT CCCGGGAGCT GGAGGAGATC AGCGAGAGGC TGGAAGAAGC CGGTGGGGCC 
ACCTCAGCCC AGATTGAGAT GAACAAGAAG CGGGAGGCTG AGTTCCAGAA AATGCGCAGG 
GACCTGGAGG AGGCCACCCT ACAGCATGAA GCCACGGCGG CCACCCTGAG GAAGAAGCAT 
GCAGATAGTG TGGCCGAGCT TGGGGAGCAG ATTGACAACC TGCAGCGAGT GAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GATGAAGATG GAGATCGATG ACCTTGCTAG TAACATGGAG 
ACTGTCTCCA AAGCCAAGGG AAACCTTGAA AAGATGTGCC GCGCTCTAGA AGATCAACTG 
AGTGAAATTA AGACCAAGGA AGAGGAGCAG CAGCGGCTGA TCAATGACCT CACAGCACAG 
AGAGCGCGCC TGCAAACAGA ATCAGGTGAA TATTCACGCC AGCTAGATGA AAAGGACACA 
CTAGTTTCAC AGCTCTCGAG GGGCAAACAA GCCTTTACAC AACAGATTGA GGAACTGAAA 
AGGCAACTTG AAGAGGAGAT AAAGGCCAAG AGTGCCCTGG CACATGCCCT GCAGTCCTCC 
CGCCATGACT GTGACCTGCT GCGGGAACAG TATGAGGAGG AGCAGGAAGC CAAGGCCGAG 
CTACAGAGAG CAATGTCCAA GGCCAACAGT GAGGTTGCCC AGTGGAGGAC CAAATATGAG 
ACAGATGCCA TCCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT GGCTCAGCGT 
CTGCAGGATG CTGAGGAACA TGTAGAAGCT GTGAATGCCA AATGTGCTTC CCTTGAGAAG 
ACGAAGCAGA GGCTCCAGAA TGAAGTTGAG GACCTCATGA TTGATGTTGA GAGGACAAAT 
GCTGCCTGTG CCGCCCTGGA CAAAAAGCAA AGGAACTTTG ATAAGATCCT GGCAGAATGG 
AAACAGAAGT GTGAAGAAAC TCATGCTGAA CTTGAAGCTT CTCAAAAGGA ATCCCGCTCA 
CTCAGCACAG AACTATTTAA GATTAAGAAT GCTTATGAGG AATCTTTAGA CCAACTTGAA 
ACCTTGAAAC GGGAAAATAA GAATTTGCAA CAGGAGATTT CTGATCTCAC TGAACAGATT 
GCAGAAGGAG GAAAGCGCAT CCATGAACTG GAAAAAATAA AGAAGCAAGT TGAGCAAGAA 
AAGTCTGAAC TTCAGGCTGC CTTAGAGGAG GCAGAGGCAT CTCTTGAACA TGAAGAGGGA 
AAGATCCTGC GCATCCAGCT TGAGTTGAAC CAAGTCAAGT CTGAGGTTGA TAGGAAAATT 
GCTGAAAAAG ATGAGGAAAT TGACCAGATG AAGAGAAACC ACATTAGAAT CGTGGAGTCC 
ATGCAGAGCA CACTGGATGC TGAGATCAGG AGCAGGAATG ATGCCATTAG GCTCAAGAAG 
AAGATGGAGG GAGACCTCAA TGAAATGGAA ATCCAGCTGA ACCATGCCAA CCGCATGGCT 
GCTGAGGCCC TGAGGAACTA TAGGAACACC CAAGCCATCC TCAAGGATAC CCAGCTCCAC 
CTGGATGATG CTCTCCGGAG CCAGGAGGAC CTGAAGGAAC AGCTGGCTAT GGTGGAGCGC 
AGAGCCAACC TGCTGCAGGC TGAGATCGAG GAGCTGCGGG CCACTCTGGA ACAGACAGAG 
AGGAGCAGGA AAATCGCAGA ACAGGAGCTC CTGGATGCCA GTGAGCGTGT TCAGCTCCTG 
CACACCCAGA ACACCAGCCT GATCAACACC AAGAAGAAGC TGGAGACAGA CATTTCCCAA 
ATCCAGGGAG AGATGGAAGA CATCATCCAG GAAGCCCGCA ATGCAGAAGA GAAGGCCAAG 
AAGGCCATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAACA GGACACCAGC 
GCCCATCTGG AGCGGATGAA GAAGAACCTG GAACAGACGG TGAAGGACCT GCAGCATCGT 
CTGGATGAGG CTGAGCAGCT GGCCCTGAAG GGTGGGAAGA AGCAGATCCA GAAACTGGAG 
GCCAGGGTTC GTGAACTTGA AGGTGAAGTT GAAAGTGAAC AGAAGCGCAA TGTTGAAGCT 
GTCAAGGGTC TACGCAAACA TGAGAGAAAA GTGAAGGAAC TCACTTACCA AACTGAGGAA 
GACCGCAAGA ATATTCTCAG GCTCCAGGAC CTGGTGGACA AACTGCAAGC AAAGGTGAAA 
TCCTACAAGA GACAAGCTGA AGAAGCGGAG GAACAATCCA ACGTCAACCT CTCCAAATTC 
CGCAGGATCC AGCACGAGCT GGAGGAGGCC GAGGAACGGG CTGACATTGC TGAGTCCCAG 
GTCAACAAGC TGAGGGTGAA GAGCAGGGAG GTTCACACAA AAATCATAAG TGAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005954.3
CDS	95..5914
Translation	MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEVTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIEILGFTSDERVSIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQNLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNAVGALAKAVYDKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKPEAHFSLIHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLALLFVGATGAEAEAGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQAMCRGFLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADSLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKIRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSGLQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMETVSKAKGNLEKMCRALEDQLSEIKTKEEEQQRLINDLTAQRARLQTESGEYSRQLDEKDTLVSQLSRGKQAFTQQIEELKRQLEEEIKAKSALAHALQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILAEWKQKCEETHAELEASQKESRSLSTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKSELQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQMKRNHIRIVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQAILKDTQLHLDDALRSQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNVEAVKGLRKHERKVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNVNLSKFRRIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE

Target ORF information:

RefSeq Version	NM_005963.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 1 (MYH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005963.3

ORF Insert Sequence:

ATGAGTTCCG ACTCTGAGAT GGCCATTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAGGGAGC GAATTGAAGC CCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTGGACCCTA AGGAGTCCTT TGTGAAAGCA ACAGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCTAAGA CCGAAGCTGG AGCTACTGTA ACAGTGAAAG ATGACCAAGT CTTCCCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAC ATGGCCATGA TGACTCATCT ACACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGCTAC GCAGCCTGGA TGATCTACAC CTACTCAGGC 
TTGTTCTGTG TCACTGTCAA CCCCTACAAG TGGTTGCCAG TGTATAATGC AGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGCGCAG GGAAGACTGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGGG AGAAGAAGAA GGAAGAAGTT ACTTCTGGCA AAATGCAGGG GACTCTGGAA 
GATCAAATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTCGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTTAC TTTCCAGCTA 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGATCTA 
ATTGAAATGC TCCTGATCAC CACCAACCCA TACGATTATG CCTTCGTCAG TCAAGGGGAG 
ATCACAGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CTACAGATAG TGCCATTGAA 
ATTCTGGGCT TTACTTCAGA TGAAAGAGTG TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAGGC AGCCTATCTC CAAAATCTGA ACTCTGCAGA TCTGCTCAAA 
GCCCTCTGCT ACCCTAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG TCAAACTGTG 
CAGCAGGTGT ACAATGCAGT GGGTGCTCTG GCCAAAGCTG TCTACGATAA GATGTTCTTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATT 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAG TGGACGTTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAACATCTT 
GGAAAATCCA ATAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGCCTGA GGCCCACTTC 
TCTTTGATTC ACTATGCTGG CACCGTGGAC TACAACATTG CCGGCTGGCT TGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTG 
GCTCTCCTCT TTGTTGGGGC AACGGGAGCG GAAGCAGAGG CTGGCGGTGG AAAGAAAGGT 
GGTAAGAAGA AGGGTTCTTC TTTCCAGACT GTGTCTGCTC TCTTCAGGGA GAATTTGAAT 
AAGCTGATGA CCAACTTGAG GAGCACTCAC CCCCACTTTG TGCGGTGCAT CATCCCCAAT 
GAAACTAAAA CTCCTGGTGC CATGGAGCAT GAGCTTGTCC TGCATCAGCT GAGGTGTAAC 
GGTGTGCTGG AAGGCATCCG CATCTGCAGG AAAGGCTTCC CAAGCAGAAT CCTTTATGCA 
GACTTCAAAC AGAGATACAA GGTGTTAAAT GCAAGTGCTA TCCCTGAAGG ACAATTCATC 
GATAGCAAGA AGGCTTCAGA GAAGCTCCTG GGGTCCATTG ACATTGACCA CACCCAGTAT 
AAATTTGGTC ACACCAAGGT CTTTTTCAAA GCTGGTCTTC TGGGGCTCCT AGAGGAGATG 
CGAGATGAGA AGCTGGCCCA GCTGATTACC CGAACCCAGG CCATGTGCAG AGGGTTCTTG 
GCAAGAGTGG AGTACCAGAA AATGGTGGAA AGAAGAGAGT CCATCTTCTG CATCCAGTAC 
AATGTCCGTG CCTTCATGAA TGTGAAGCAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAACCCCTCC TCAAAAGTGC AGAGACAGAG AAGGAGATGG CCAACATGAA GGAAGAATTT 
GAGAAAACCA AAGAAGAGCT GGCTAAGACC GAGGCAAAAA GGAAAGAGCT GGAAGAAAAA 
ATGGTTACTC TGATGCAAGA AAAAAATGAC TTGCAACTCC AGGTTCAAGC TGAAGCTGAC 
AGCTTGGCTG ATGCAGAGGA AAGGTGTGAC CAGCTAATCA AAACCAAAAT CCAGCTAGAA 
GCCAAAATCA AAGAGGTGAC TGAGAGAGCT GAGGATGAGG AAGAGATCAA TGCTGAGCTG 
ACAGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAAC TCAAGAAAGA CATTGATGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAACATG CCACAGAAAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC GGGTCTGGAT GAAACCATTG CTAAGCTGAC CAAGGAGAAG 
AAGGCTCTCC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGGCAGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC TAAAATCAAA CTTGAACAAC AAGTGGATGA TCTTGAAGGA 
TCTTTGGAAC AAGAAAAGAA AATCCGGATG GATCTAGAAA GAGCAAAGAG AAAACTAGAG 
GGAGACCTAA AATTGGCTCA AGAATCCACA ATGGATATAG AAAATGACAA ACAACAACTT 
GATGAAAAGC TTAAAAAGAA AGAGTTTGAA ATGAGCGGTC TGCAAAGCAA GATTGAAGAT 
GAACAAGCCC TTGGTATGCA GCTGCAGAAG AAAATCAAGG AGTTACAAGC CCGCATTGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCCGGG CCAAAGCAGA GAAGCAGCGC 
TCTGATCTCT CCCGGGAGCT GGAGGAGATC AGCGAGAGGC TGGAAGAAGC CGGTGGGGCC 
ACCTCAGCCC AGATTGAGAT GAACAAGAAG CGGGAGGCTG AGTTCCAGAA AATGCGCAGG 
GACCTGGAGG AGGCCACCCT ACAGCATGAA GCCACGGCGG CCACCCTGAG GAAGAAGCAT 
GCAGATAGTG TGGCCGAGCT TGGGGAGCAG ATTGACAACC TGCAGCGAGT GAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GATGAAGATG GAGATCGATG ACCTTGCTAG TAACATGGAG 
ACTGTCTCCA AAGCCAAGGG AAACCTTGAA AAGATGTGCC GCGCTCTAGA AGATCAACTG 
AGTGAAATTA AGACCAAGGA AGAGGAGCAG CAGCGGCTGA TCAATGACCT CACAGCACAG 
AGAGCGCGCC TGCAAACAGA ATCAGGTGAA TATTCACGCC AGCTAGATGA AAAGGACACA 
CTAGTTTCAC AGCTCTCGAG GGGCAAACAA GCCTTTACAC AACAGATTGA GGAACTGAAA 
AGGCAACTTG AAGAGGAGAT AAAGGCCAAG AGTGCCCTGG CACATGCCCT GCAGTCCTCC 
CGCCATGACT GTGACCTGCT GCGGGAACAG TATGAGGAGG AGCAGGAAGC CAAGGCCGAG 
CTACAGAGAG CAATGTCCAA GGCCAACAGT GAGGTTGCCC AGTGGAGGAC CAAATATGAG 
ACAGATGCCA TCCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT GGCTCAGCGT 
CTGCAGGATG CTGAGGAACA TGTAGAAGCT GTGAATGCCA AATGTGCTTC CCTTGAGAAG 
ACGAAGCAGA GGCTCCAGAA TGAAGTTGAG GACCTCATGA TTGATGTTGA GAGGACAAAT 
GCTGCCTGTG CCGCCCTGGA CAAAAAGCAA AGGAACTTTG ATAAGATCCT GGCAGAATGG 
AAACAGAAGT GTGAAGAAAC TCATGCTGAA CTTGAAGCTT CTCAAAAGGA ATCCCGCTCA 
CTCAGCACAG AACTATTTAA GATTAAGAAT GCTTATGAGG AATCTTTAGA CCAACTTGAA 
ACCTTGAAAC GGGAAAATAA GAATTTGCAA CAGGAGATTT CTGATCTCAC TGAACAGATT 
GCAGAAGGAG GAAAGCGCAT CCATGAACTG GAAAAAATAA AGAAGCAAGT TGAGCAAGAA 
AAGTCTGAAC TTCAGGCTGC CTTAGAGGAG GCAGAGGCAT CTCTTGAACA TGAAGAGGGA 
AAGATCCTGC GCATCCAGCT TGAGTTGAAC CAAGTCAAGT CTGAGGTTGA TAGGAAAATT 
GCTGAAAAAG ATGAGGAAAT TGACCAGATG AAGAGAAACC ACATTAGAAT CGTGGAGTCC 
ATGCAGAGCA CACTGGATGC TGAGATCAGG AGCAGGAATG ATGCCATTAG GCTCAAGAAG 
AAGATGGAGG GAGACCTCAA TGAAATGGAA ATCCAGCTGA ACCATGCCAA CCGCATGGCT 
GCTGAGGCCC TGAGGAACTA TAGGAACACC CAAGCCATCC TCAAGGATAC CCAGCTCCAC 
CTGGATGATG CTCTCCGGAG CCAGGAGGAC CTGAAGGAAC AGCTGGCTAT GGTGGAGCGC 
AGAGCCAACC TGCTGCAGGC TGAGATCGAG GAGCTGCGGG CCACTCTGGA ACAGACAGAG 
AGGAGCAGGA AAATCGCAGA ACAGGAGCTC CTGGATGCCA GTGAGCGTGT TCAGCTCCTG 
CACACCCAGA ACACCAGCCT GATCAACACC AAGAAGAAGC TGGAGACAGA CATTTCCCAA 
ATCCAGGGAG AGATGGAAGA CATCATCCAG GAAGCCCGCA ATGCAGAAGA GAAGGCCAAG 
AAGGCCATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAACA GGACACCAGC 
GCCCATCTGG AGCGGATGAA GAAGAACCTG GAACAGACGG TGAAGGACCT GCAGCATCGT 
CTGGATGAGG CTGAGCAGCT GGCCCTGAAG GGTGGGAAGA AGCAGATCCA GAAACTGGAG 
GCCAGGGTTC GTGAACTTGA AGGTGAAGTT GAAAGTGAAC AGAAGCGCAA TGTTGAAGCT 
GTCAAGGGTC TACGCAAACA TGAGAGAAAA GTGAAGGAAC TCACTTACCA AACTGAGGAA 
GACCGCAAGA ATATTCTCAG GCTCCAGGAC CTGGTGGACA AACTGCAAGC AAAGGTGAAA 
TCCTACAAGA GACAAGCTGA AGAAGCGGAG GAACAATCCA ACGTCAACCT CTCCAAATTC 
CGCAGGATCC AGCACGAGCT GGAGGAGGCC GAGGAACGGG CTGACATTGC TGAGTCCCAG 
GTCAACAAGC TGAGGGTGAA GAGCAGGGAG GTTCACACAA AAATCATAAG TGAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH1 ( NM_005963.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005954.3 Homo sapiens myosin heavy chain 1 (MYH1), mRNA.

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CloneID	OHu05264
Clone ID Related Accession (Same CDS sequence)	XM_017024675.1 , NM_005963.3 , NM_005963.4
Accession Version	NM_005963.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	myosin-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA826478.1, BC114545.1, AF111785.1 and AJ573764.1. On Nov 22, 2018 this sequence version replaced NM_005963.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF111785.1, EU794637.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000226207.6/ ENSP00000226207.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTTCCG ACTCTGAGAT GGCCATTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAGGGAGC GAATTGAAGC CCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTGGACCCTA AGGAGTCCTT TGTGAAAGCA ACAGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCTAAGA CCGAAGCTGG AGCTACTGTA ACAGTGAAAG ATGACCAAGT CTTCCCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAC ATGGCCATGA TGACTCATCT ACACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGCTAC GCAGCCTGGA TGATCTACAC CTACTCAGGC 
TTGTTCTGTG TCACTGTCAA CCCCTACAAG TGGTTGCCAG TGTATAATGC AGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGCGCAG GGAAGACTGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGGG AGAAGAAGAA GGAAGAAGTT ACTTCTGGCA AAATGCAGGG GACTCTGGAA 
GATCAAATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTCGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTTAC TTTCCAGCTA 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGATCTA 
ATTGAAATGC TCCTGATCAC CACCAACCCA TACGATTATG CCTTCGTCAG TCAAGGGGAG 
ATCACAGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CTACAGATAG TGCCATTGAA 
ATTCTGGGCT TTACTTCAGA TGAAAGAGTG TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAGGC AGCCTATCTC CAAAATCTGA ACTCTGCAGA TCTGCTCAAA 
GCCCTCTGCT ACCCTAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG TCAAACTGTG 
CAGCAGGTGT ACAATGCAGT GGGTGCTCTG GCCAAAGCTG TCTACGATAA GATGTTCTTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATT 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAG TGGACGTTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAACATCTT 
GGAAAATCCA ATAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGCCTGA GGCCCACTTC 
TCTTTGATTC ACTATGCTGG CACCGTGGAC TACAACATTG CCGGCTGGCT TGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTG 
GCTCTCCTCT TTGTTGGGGC AACGGGAGCG GAAGCAGAGG CTGGCGGTGG AAAGAAAGGT 
GGTAAGAAGA AGGGTTCTTC TTTCCAGACT GTGTCTGCTC TCTTCAGGGA GAATTTGAAT 
AAGCTGATGA CCAACTTGAG GAGCACTCAC CCCCACTTTG TGCGGTGCAT CATCCCCAAT 
GAAACTAAAA CTCCTGGTGC CATGGAGCAT GAGCTTGTCC TGCATCAGCT GAGGTGTAAC 
GGTGTGCTGG AAGGCATCCG CATCTGCAGG AAAGGCTTCC CAAGCAGAAT CCTTTATGCA 
GACTTCAAAC AGAGATACAA GGTGTTAAAT GCAAGTGCTA TCCCTGAAGG ACAATTCATC 
GATAGCAAGA AGGCTTCAGA GAAGCTCCTG GGGTCCATTG ACATTGACCA CACCCAGTAT 
AAATTTGGTC ACACCAAGGT CTTTTTCAAA GCTGGTCTTC TGGGGCTCCT AGAGGAGATG 
CGAGATGAGA AGCTGGCCCA GCTGATTACC CGAACCCAGG CCATGTGCAG AGGGTTCTTG 
GCAAGAGTGG AGTACCAGAA AATGGTGGAA AGAAGAGAGT CCATCTTCTG CATCCAGTAC 
AATGTCCGTG CCTTCATGAA TGTGAAGCAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAACCCCTCC TCAAAAGTGC AGAGACAGAG AAGGAGATGG CCAACATGAA GGAAGAATTT 
GAGAAAACCA AAGAAGAGCT GGCTAAGACC GAGGCAAAAA GGAAAGAGCT GGAAGAAAAA 
ATGGTTACTC TGATGCAAGA AAAAAATGAC TTGCAACTCC AGGTTCAAGC TGAAGCTGAC 
AGCTTGGCTG ATGCAGAGGA AAGGTGTGAC CAGCTAATCA AAACCAAAAT CCAGCTAGAA 
GCCAAAATCA AAGAGGTGAC TGAGAGAGCT GAGGATGAGG AAGAGATCAA TGCTGAGCTG 
ACAGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAAC TCAAGAAAGA CATTGATGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAACATG CCACAGAAAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC GGGTCTGGAT GAAACCATTG CTAAGCTGAC CAAGGAGAAG 
AAGGCTCTCC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGGCAGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC TAAAATCAAA CTTGAACAAC AAGTGGATGA TCTTGAAGGA 
TCTTTGGAAC AAGAAAAGAA AATCCGGATG GATCTAGAAA GAGCAAAGAG AAAACTAGAG 
GGAGACCTAA AATTGGCTCA AGAATCCACA ATGGATATAG AAAATGACAA ACAACAACTT 
GATGAAAAGC TTAAAAAGAA AGAGTTTGAA ATGAGCGGTC TGCAAAGCAA GATTGAAGAT 
GAACAAGCCC TTGGTATGCA GCTGCAGAAG AAAATCAAGG AGTTACAAGC CCGCATTGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCCGGG CCAAAGCAGA GAAGCAGCGC 
TCTGATCTCT CCCGGGAGCT GGAGGAGATC AGCGAGAGGC TGGAAGAAGC CGGTGGGGCC 
ACCTCAGCCC AGATTGAGAT GAACAAGAAG CGGGAGGCTG AGTTCCAGAA AATGCGCAGG 
GACCTGGAGG AGGCCACCCT ACAGCATGAA GCCACGGCGG CCACCCTGAG GAAGAAGCAT 
GCAGATAGTG TGGCCGAGCT TGGGGAGCAG ATTGACAACC TGCAGCGAGT GAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GATGAAGATG GAGATCGATG ACCTTGCTAG TAACATGGAG 
ACTGTCTCCA AAGCCAAGGG AAACCTTGAA AAGATGTGCC GCGCTCTAGA AGATCAACTG 
AGTGAAATTA AGACCAAGGA AGAGGAGCAG CAGCGGCTGA TCAATGACCT CACAGCACAG 
AGAGCGCGCC TGCAAACAGA ATCAGGTGAA TATTCACGCC AGCTAGATGA AAAGGACACA 
CTAGTTTCAC AGCTCTCGAG GGGCAAACAA GCCTTTACAC AACAGATTGA GGAACTGAAA 
AGGCAACTTG AAGAGGAGAT AAAGGCCAAG AGTGCCCTGG CACATGCCCT GCAGTCCTCC 
CGCCATGACT GTGACCTGCT GCGGGAACAG TATGAGGAGG AGCAGGAAGC CAAGGCCGAG 
CTACAGAGAG CAATGTCCAA GGCCAACAGT GAGGTTGCCC AGTGGAGGAC CAAATATGAG 
ACAGATGCCA TCCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT GGCTCAGCGT 
CTGCAGGATG CTGAGGAACA TGTAGAAGCT GTGAATGCCA AATGTGCTTC CCTTGAGAAG 
ACGAAGCAGA GGCTCCAGAA TGAAGTTGAG GACCTCATGA TTGATGTTGA GAGGACAAAT 
GCTGCCTGTG CCGCCCTGGA CAAAAAGCAA AGGAACTTTG ATAAGATCCT GGCAGAATGG 
AAACAGAAGT GTGAAGAAAC TCATGCTGAA CTTGAAGCTT CTCAAAAGGA ATCCCGCTCA 
CTCAGCACAG AACTATTTAA GATTAAGAAT GCTTATGAGG AATCTTTAGA CCAACTTGAA 
ACCTTGAAAC GGGAAAATAA GAATTTGCAA CAGGAGATTT CTGATCTCAC TGAACAGATT 
GCAGAAGGAG GAAAGCGCAT CCATGAACTG GAAAAAATAA AGAAGCAAGT TGAGCAAGAA 
AAGTCTGAAC TTCAGGCTGC CTTAGAGGAG GCAGAGGCAT CTCTTGAACA TGAAGAGGGA 
AAGATCCTGC GCATCCAGCT TGAGTTGAAC CAAGTCAAGT CTGAGGTTGA TAGGAAAATT 
GCTGAAAAAG ATGAGGAAAT TGACCAGATG AAGAGAAACC ACATTAGAAT CGTGGAGTCC 
ATGCAGAGCA CACTGGATGC TGAGATCAGG AGCAGGAATG ATGCCATTAG GCTCAAGAAG 
AAGATGGAGG GAGACCTCAA TGAAATGGAA ATCCAGCTGA ACCATGCCAA CCGCATGGCT 
GCTGAGGCCC TGAGGAACTA TAGGAACACC CAAGCCATCC TCAAGGATAC CCAGCTCCAC 
CTGGATGATG CTCTCCGGAG CCAGGAGGAC CTGAAGGAAC AGCTGGCTAT GGTGGAGCGC 
AGAGCCAACC TGCTGCAGGC TGAGATCGAG GAGCTGCGGG CCACTCTGGA ACAGACAGAG 
AGGAGCAGGA AAATCGCAGA ACAGGAGCTC CTGGATGCCA GTGAGCGTGT TCAGCTCCTG 
CACACCCAGA ACACCAGCCT GATCAACACC AAGAAGAAGC TGGAGACAGA CATTTCCCAA 
ATCCAGGGAG AGATGGAAGA CATCATCCAG GAAGCCCGCA ATGCAGAAGA GAAGGCCAAG 
AAGGCCATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAACA GGACACCAGC 
GCCCATCTGG AGCGGATGAA GAAGAACCTG GAACAGACGG TGAAGGACCT GCAGCATCGT 
CTGGATGAGG CTGAGCAGCT GGCCCTGAAG GGTGGGAAGA AGCAGATCCA GAAACTGGAG 
GCCAGGGTTC GTGAACTTGA AGGTGAAGTT GAAAGTGAAC AGAAGCGCAA TGTTGAAGCT 
GTCAAGGGTC TACGCAAACA TGAGAGAAAA GTGAAGGAAC TCACTTACCA AACTGAGGAA 
GACCGCAAGA ATATTCTCAG GCTCCAGGAC CTGGTGGACA AACTGCAAGC AAAGGTGAAA 
TCCTACAAGA GACAAGCTGA AGAAGCGGAG GAACAATCCA ACGTCAACCT CTCCAAATTC 
CGCAGGATCC AGCACGAGCT GGAGGAGGCC GAGGAACGGG CTGACATTGC TGAGTCCCAG 
GTCAACAAGC TGAGGGTGAA GAGCAGGGAG GTTCACACAA AAATCATAAG TGAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005954.3
CDS	95..5914
Translation	MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEVTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIEILGFTSDERVSIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQNLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNAVGALAKAVYDKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKPEAHFSLIHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLALLFVGATGAEAEAGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQAMCRGFLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADSLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKIRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSGLQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMETVSKAKGNLEKMCRALEDQLSEIKTKEEEQQRLINDLTAQRARLQTESGEYSRQLDEKDTLVSQLSRGKQAFTQQIEELKRQLEEEIKAKSALAHALQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILAEWKQKCEETHAELEASQKESRSLSTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKSELQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQMKRNHIRIVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQAILKDTQLHLDDALRSQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNVEAVKGLRKHERKVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNVNLSKFRRIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE

Target ORF information:

RefSeq Version	NM_005963.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 1 (MYH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005963.4

ORF Insert Sequence:

ATGAGTTCCG ACTCTGAGAT GGCCATTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAGGGAGC GAATTGAAGC CCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTGGACCCTA AGGAGTCCTT TGTGAAAGCA ACAGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCTAAGA CCGAAGCTGG AGCTACTGTA ACAGTGAAAG ATGACCAAGT CTTCCCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAC ATGGCCATGA TGACTCATCT ACACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGCTAC GCAGCCTGGA TGATCTACAC CTACTCAGGC 
TTGTTCTGTG TCACTGTCAA CCCCTACAAG TGGTTGCCAG TGTATAATGC AGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGCGCAG GGAAGACTGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGGG AGAAGAAGAA GGAAGAAGTT ACTTCTGGCA AAATGCAGGG GACTCTGGAA 
GATCAAATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTCGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTTAC TTTCCAGCTA 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGATCTA 
ATTGAAATGC TCCTGATCAC CACCAACCCA TACGATTATG CCTTCGTCAG TCAAGGGGAG 
ATCACAGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CTACAGATAG TGCCATTGAA 
ATTCTGGGCT TTACTTCAGA TGAAAGAGTG TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAGGC AGCCTATCTC CAAAATCTGA ACTCTGCAGA TCTGCTCAAA 
GCCCTCTGCT ACCCTAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG TCAAACTGTG 
CAGCAGGTGT ACAATGCAGT GGGTGCTCTG GCCAAAGCTG TCTACGATAA GATGTTCTTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATT 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAG TGGACGTTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAACATCTT 
GGAAAATCCA ATAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGCCTGA GGCCCACTTC 
TCTTTGATTC ACTATGCTGG CACCGTGGAC TACAACATTG CCGGCTGGCT TGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTG 
GCTCTCCTCT TTGTTGGGGC AACGGGAGCG GAAGCAGAGG CTGGCGGTGG AAAGAAAGGT 
GGTAAGAAGA AGGGTTCTTC TTTCCAGACT GTGTCTGCTC TCTTCAGGGA GAATTTGAAT 
AAGCTGATGA CCAACTTGAG GAGCACTCAC CCCCACTTTG TGCGGTGCAT CATCCCCAAT 
GAAACTAAAA CTCCTGGTGC CATGGAGCAT GAGCTTGTCC TGCATCAGCT GAGGTGTAAC 
GGTGTGCTGG AAGGCATCCG CATCTGCAGG AAAGGCTTCC CAAGCAGAAT CCTTTATGCA 
GACTTCAAAC AGAGATACAA GGTGTTAAAT GCAAGTGCTA TCCCTGAAGG ACAATTCATC 
GATAGCAAGA AGGCTTCAGA GAAGCTCCTG GGGTCCATTG ACATTGACCA CACCCAGTAT 
AAATTTGGTC ACACCAAGGT CTTTTTCAAA GCTGGTCTTC TGGGGCTCCT AGAGGAGATG 
CGAGATGAGA AGCTGGCCCA GCTGATTACC CGAACCCAGG CCATGTGCAG AGGGTTCTTG 
GCAAGAGTGG AGTACCAGAA AATGGTGGAA AGAAGAGAGT CCATCTTCTG CATCCAGTAC 
AATGTCCGTG CCTTCATGAA TGTGAAGCAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAACCCCTCC TCAAAAGTGC AGAGACAGAG AAGGAGATGG CCAACATGAA GGAAGAATTT 
GAGAAAACCA AAGAAGAGCT GGCTAAGACC GAGGCAAAAA GGAAAGAGCT GGAAGAAAAA 
ATGGTTACTC TGATGCAAGA AAAAAATGAC TTGCAACTCC AGGTTCAAGC TGAAGCTGAC 
AGCTTGGCTG ATGCAGAGGA AAGGTGTGAC CAGCTAATCA AAACCAAAAT CCAGCTAGAA 
GCCAAAATCA AAGAGGTGAC TGAGAGAGCT GAGGATGAGG AAGAGATCAA TGCTGAGCTG 
ACAGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAAC TCAAGAAAGA CATTGATGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAACATG CCACAGAAAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC GGGTCTGGAT GAAACCATTG CTAAGCTGAC CAAGGAGAAG 
AAGGCTCTCC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGGCAGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC TAAAATCAAA CTTGAACAAC AAGTGGATGA TCTTGAAGGA 
TCTTTGGAAC AAGAAAAGAA AATCCGGATG GATCTAGAAA GAGCAAAGAG AAAACTAGAG 
GGAGACCTAA AATTGGCTCA AGAATCCACA ATGGATATAG AAAATGACAA ACAACAACTT 
GATGAAAAGC TTAAAAAGAA AGAGTTTGAA ATGAGCGGTC TGCAAAGCAA GATTGAAGAT 
GAACAAGCCC TTGGTATGCA GCTGCAGAAG AAAATCAAGG AGTTACAAGC CCGCATTGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCCGGG CCAAAGCAGA GAAGCAGCGC 
TCTGATCTCT CCCGGGAGCT GGAGGAGATC AGCGAGAGGC TGGAAGAAGC CGGTGGGGCC 
ACCTCAGCCC AGATTGAGAT GAACAAGAAG CGGGAGGCTG AGTTCCAGAA AATGCGCAGG 
GACCTGGAGG AGGCCACCCT ACAGCATGAA GCCACGGCGG CCACCCTGAG GAAGAAGCAT 
GCAGATAGTG TGGCCGAGCT TGGGGAGCAG ATTGACAACC TGCAGCGAGT GAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GATGAAGATG GAGATCGATG ACCTTGCTAG TAACATGGAG 
ACTGTCTCCA AAGCCAAGGG AAACCTTGAA AAGATGTGCC GCGCTCTAGA AGATCAACTG 
AGTGAAATTA AGACCAAGGA AGAGGAGCAG CAGCGGCTGA TCAATGACCT CACAGCACAG 
AGAGCGCGCC TGCAAACAGA ATCAGGTGAA TATTCACGCC AGCTAGATGA AAAGGACACA 
CTAGTTTCAC AGCTCTCGAG GGGCAAACAA GCCTTTACAC AACAGATTGA GGAACTGAAA 
AGGCAACTTG AAGAGGAGAT AAAGGCCAAG AGTGCCCTGG CACATGCCCT GCAGTCCTCC 
CGCCATGACT GTGACCTGCT GCGGGAACAG TATGAGGAGG AGCAGGAAGC CAAGGCCGAG 
CTACAGAGAG CAATGTCCAA GGCCAACAGT GAGGTTGCCC AGTGGAGGAC CAAATATGAG 
ACAGATGCCA TCCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT GGCTCAGCGT 
CTGCAGGATG CTGAGGAACA TGTAGAAGCT GTGAATGCCA AATGTGCTTC CCTTGAGAAG 
ACGAAGCAGA GGCTCCAGAA TGAAGTTGAG GACCTCATGA TTGATGTTGA GAGGACAAAT 
GCTGCCTGTG CCGCCCTGGA CAAAAAGCAA AGGAACTTTG ATAAGATCCT GGCAGAATGG 
AAACAGAAGT GTGAAGAAAC TCATGCTGAA CTTGAAGCTT CTCAAAAGGA ATCCCGCTCA 
CTCAGCACAG AACTATTTAA GATTAAGAAT GCTTATGAGG AATCTTTAGA CCAACTTGAA 
ACCTTGAAAC GGGAAAATAA GAATTTGCAA CAGGAGATTT CTGATCTCAC TGAACAGATT 
GCAGAAGGAG GAAAGCGCAT CCATGAACTG GAAAAAATAA AGAAGCAAGT TGAGCAAGAA 
AAGTCTGAAC TTCAGGCTGC CTTAGAGGAG GCAGAGGCAT CTCTTGAACA TGAAGAGGGA 
AAGATCCTGC GCATCCAGCT TGAGTTGAAC CAAGTCAAGT CTGAGGTTGA TAGGAAAATT 
GCTGAAAAAG ATGAGGAAAT TGACCAGATG AAGAGAAACC ACATTAGAAT CGTGGAGTCC 
ATGCAGAGCA CACTGGATGC TGAGATCAGG AGCAGGAATG ATGCCATTAG GCTCAAGAAG 
AAGATGGAGG GAGACCTCAA TGAAATGGAA ATCCAGCTGA ACCATGCCAA CCGCATGGCT 
GCTGAGGCCC TGAGGAACTA TAGGAACACC CAAGCCATCC TCAAGGATAC CCAGCTCCAC 
CTGGATGATG CTCTCCGGAG CCAGGAGGAC CTGAAGGAAC AGCTGGCTAT GGTGGAGCGC 
AGAGCCAACC TGCTGCAGGC TGAGATCGAG GAGCTGCGGG CCACTCTGGA ACAGACAGAG 
AGGAGCAGGA AAATCGCAGA ACAGGAGCTC CTGGATGCCA GTGAGCGTGT TCAGCTCCTG 
CACACCCAGA ACACCAGCCT GATCAACACC AAGAAGAAGC TGGAGACAGA CATTTCCCAA 
ATCCAGGGAG AGATGGAAGA CATCATCCAG GAAGCCCGCA ATGCAGAAGA GAAGGCCAAG 
AAGGCCATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAACA GGACACCAGC 
GCCCATCTGG AGCGGATGAA GAAGAACCTG GAACAGACGG TGAAGGACCT GCAGCATCGT 
CTGGATGAGG CTGAGCAGCT GGCCCTGAAG GGTGGGAAGA AGCAGATCCA GAAACTGGAG 
GCCAGGGTTC GTGAACTTGA AGGTGAAGTT GAAAGTGAAC AGAAGCGCAA TGTTGAAGCT 
GTCAAGGGTC TACGCAAACA TGAGAGAAAA GTGAAGGAAC TCACTTACCA AACTGAGGAA 
GACCGCAAGA ATATTCTCAG GCTCCAGGAC CTGGTGGACA AACTGCAAGC AAAGGTGAAA 
TCCTACAAGA GACAAGCTGA AGAAGCGGAG GAACAATCCA ACGTCAACCT CTCCAAATTC 
CGCAGGATCC AGCACGAGCT GGAGGAGGCC GAGGAACGGG CTGACATTGC TGAGTCCCAG 
GTCAACAAGC TGAGGGTGAA GAGCAGGGAG GTTCACACAA AAATCATAAG TGAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH1 ( XM_017024675.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880164.1 Homo sapiens myosin heavy chain 1 (MYH1), transcript variant X1, mRNA.

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CloneID	OHu05264
Clone ID Related Accession (Same CDS sequence)	XM_017024675.1 , NM_005963.3 , NM_005963.4
Accession Version	XM_017024675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myosin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCG ACTCTGAGAT GGCCATTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAGGGAGC GAATTGAAGC CCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTGGACCCTA AGGAGTCCTT TGTGAAAGCA ACAGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCTAAGA CCGAAGCTGG AGCTACTGTA ACAGTGAAAG ATGACCAAGT CTTCCCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAC ATGGCCATGA TGACTCATCT ACACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGCTAC GCAGCCTGGA TGATCTACAC CTACTCAGGC 
TTGTTCTGTG TCACTGTCAA CCCCTACAAG TGGTTGCCAG TGTATAATGC AGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGCGCAG GGAAGACTGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGGG AGAAGAAGAA GGAAGAAGTT ACTTCTGGCA AAATGCAGGG GACTCTGGAA 
GATCAAATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTCGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTTAC TTTCCAGCTA 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGATCTA 
ATTGAAATGC TCCTGATCAC CACCAACCCA TACGATTATG CCTTCGTCAG TCAAGGGGAG 
ATCACAGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CTACAGATAG TGCCATTGAA 
ATTCTGGGCT TTACTTCAGA TGAAAGAGTG TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAGGC AGCCTATCTC CAAAATCTGA ACTCTGCAGA TCTGCTCAAA 
GCCCTCTGCT ACCCTAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG TCAAACTGTG 
CAGCAGGTGT ACAATGCAGT GGGTGCTCTG GCCAAAGCTG TCTACGATAA GATGTTCTTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATT 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAG TGGACGTTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAACATCTT 
GGAAAATCCA ATAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGCCTGA GGCCCACTTC 
TCTTTGATTC ACTATGCTGG CACCGTGGAC TACAACATTG CCGGCTGGCT TGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTG 
GCTCTCCTCT TTGTTGGGGC AACGGGAGCG GAAGCAGAGG CTGGCGGTGG AAAGAAAGGT 
GGTAAGAAGA AGGGTTCTTC TTTCCAGACT GTGTCTGCTC TCTTCAGGGA GAATTTGAAT 
AAGCTGATGA CCAACTTGAG GAGCACTCAC CCCCACTTTG TGCGGTGCAT CATCCCCAAT 
GAAACTAAAA CTCCTGGTGC CATGGAGCAT GAGCTTGTCC TGCATCAGCT GAGGTGTAAC 
GGTGTGCTGG AAGGCATCCG CATCTGCAGG AAAGGCTTCC CAAGCAGAAT CCTTTATGCA 
GACTTCAAAC AGAGATACAA GGTGTTAAAT GCAAGTGCTA TCCCTGAAGG ACAATTCATC 
GATAGCAAGA AGGCTTCAGA GAAGCTCCTG GGGTCCATTG ACATTGACCA CACCCAGTAT 
AAATTTGGTC ACACCAAGGT CTTTTTCAAA GCTGGTCTTC TGGGGCTCCT AGAGGAGATG 
CGAGATGAGA AGCTGGCCCA GCTGATTACC CGAACCCAGG CCATGTGCAG AGGGTTCTTG 
GCAAGAGTGG AGTACCAGAA AATGGTGGAA AGAAGAGAGT CCATCTTCTG CATCCAGTAC 
AATGTCCGTG CCTTCATGAA TGTGAAGCAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAACCCCTCC TCAAAAGTGC AGAGACAGAG AAGGAGATGG CCAACATGAA GGAAGAATTT 
GAGAAAACCA AAGAAGAGCT GGCTAAGACC GAGGCAAAAA GGAAAGAGCT GGAAGAAAAA 
ATGGTTACTC TGATGCAAGA AAAAAATGAC TTGCAACTCC AGGTTCAAGC TGAAGCTGAC 
AGCTTGGCTG ATGCAGAGGA AAGGTGTGAC CAGCTAATCA AAACCAAAAT CCAGCTAGAA 
GCCAAAATCA AAGAGGTGAC TGAGAGAGCT GAGGATGAGG AAGAGATCAA TGCTGAGCTG 
ACAGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAAC TCAAGAAAGA CATTGATGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAACATG CCACAGAAAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC GGGTCTGGAT GAAACCATTG CTAAGCTGAC CAAGGAGAAG 
AAGGCTCTCC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGGCAGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC TAAAATCAAA CTTGAACAAC AAGTGGATGA TCTTGAAGGA 
TCTTTGGAAC AAGAAAAGAA AATCCGGATG GATCTAGAAA GAGCAAAGAG AAAACTAGAG 
GGAGACCTAA AATTGGCTCA AGAATCCACA ATGGATATAG AAAATGACAA ACAACAACTT 
GATGAAAAGC TTAAAAAGAA AGAGTTTGAA ATGAGCGGTC TGCAAAGCAA GATTGAAGAT 
GAACAAGCCC TTGGTATGCA GCTGCAGAAG AAAATCAAGG AGTTACAAGC CCGCATTGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCCGGG CCAAAGCAGA GAAGCAGCGC 
TCTGATCTCT CCCGGGAGCT GGAGGAGATC AGCGAGAGGC TGGAAGAAGC CGGTGGGGCC 
ACCTCAGCCC AGATTGAGAT GAACAAGAAG CGGGAGGCTG AGTTCCAGAA AATGCGCAGG 
GACCTGGAGG AGGCCACCCT ACAGCATGAA GCCACGGCGG CCACCCTGAG GAAGAAGCAT 
GCAGATAGTG TGGCCGAGCT TGGGGAGCAG ATTGACAACC TGCAGCGAGT GAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GATGAAGATG GAGATCGATG ACCTTGCTAG TAACATGGAG 
ACTGTCTCCA AAGCCAAGGG AAACCTTGAA AAGATGTGCC GCGCTCTAGA AGATCAACTG 
AGTGAAATTA AGACCAAGGA AGAGGAGCAG CAGCGGCTGA TCAATGACCT CACAGCACAG 
AGAGCGCGCC TGCAAACAGA ATCAGGTGAA TATTCACGCC AGCTAGATGA AAAGGACACA 
CTAGTTTCAC AGCTCTCGAG GGGCAAACAA GCCTTTACAC AACAGATTGA GGAACTGAAA 
AGGCAACTTG AAGAGGAGAT AAAGGCCAAG AGTGCCCTGG CACATGCCCT GCAGTCCTCC 
CGCCATGACT GTGACCTGCT GCGGGAACAG TATGAGGAGG AGCAGGAAGC CAAGGCCGAG 
CTACAGAGAG CAATGTCCAA GGCCAACAGT GAGGTTGCCC AGTGGAGGAC CAAATATGAG 
ACAGATGCCA TCCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT GGCTCAGCGT 
CTGCAGGATG CTGAGGAACA TGTAGAAGCT GTGAATGCCA AATGTGCTTC CCTTGAGAAG 
ACGAAGCAGA GGCTCCAGAA TGAAGTTGAG GACCTCATGA TTGATGTTGA GAGGACAAAT 
GCTGCCTGTG CCGCCCTGGA CAAAAAGCAA AGGAACTTTG ATAAGATCCT GGCAGAATGG 
AAACAGAAGT GTGAAGAAAC TCATGCTGAA CTTGAAGCTT CTCAAAAGGA ATCCCGCTCA 
CTCAGCACAG AACTATTTAA GATTAAGAAT GCTTATGAGG AATCTTTAGA CCAACTTGAA 
ACCTTGAAAC GGGAAAATAA GAATTTGCAA CAGGAGATTT CTGATCTCAC TGAACAGATT 
GCAGAAGGAG GAAAGCGCAT CCATGAACTG GAAAAAATAA AGAAGCAAGT TGAGCAAGAA 
AAGTCTGAAC TTCAGGCTGC CTTAGAGGAG GCAGAGGCAT CTCTTGAACA TGAAGAGGGA 
AAGATCCTGC GCATCCAGCT TGAGTTGAAC CAAGTCAAGT CTGAGGTTGA TAGGAAAATT 
GCTGAAAAAG ATGAGGAAAT TGACCAGATG AAGAGAAACC ACATTAGAAT CGTGGAGTCC 
ATGCAGAGCA CACTGGATGC TGAGATCAGG AGCAGGAATG ATGCCATTAG GCTCAAGAAG 
AAGATGGAGG GAGACCTCAA TGAAATGGAA ATCCAGCTGA ACCATGCCAA CCGCATGGCT 
GCTGAGGCCC TGAGGAACTA TAGGAACACC CAAGCCATCC TCAAGGATAC CCAGCTCCAC 
CTGGATGATG CTCTCCGGAG CCAGGAGGAC CTGAAGGAAC AGCTGGCTAT GGTGGAGCGC 
AGAGCCAACC TGCTGCAGGC TGAGATCGAG GAGCTGCGGG CCACTCTGGA ACAGACAGAG 
AGGAGCAGGA AAATCGCAGA ACAGGAGCTC CTGGATGCCA GTGAGCGTGT TCAGCTCCTG 
CACACCCAGA ACACCAGCCT GATCAACACC AAGAAGAAGC TGGAGACAGA CATTTCCCAA 
ATCCAGGGAG AGATGGAAGA CATCATCCAG GAAGCCCGCA ATGCAGAAGA GAAGGCCAAG 
AAGGCCATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAACA GGACACCAGC 
GCCCATCTGG AGCGGATGAA GAAGAACCTG GAACAGACGG TGAAGGACCT GCAGCATCGT 
CTGGATGAGG CTGAGCAGCT GGCCCTGAAG GGTGGGAAGA AGCAGATCCA GAAACTGGAG 
GCCAGGGTTC GTGAACTTGA AGGTGAAGTT GAAAGTGAAC AGAAGCGCAA TGTTGAAGCT 
GTCAAGGGTC TACGCAAACA TGAGAGAAAA GTGAAGGAAC TCACTTACCA AACTGAGGAA 
GACCGCAAGA ATATTCTCAG GCTCCAGGAC CTGGTGGACA AACTGCAAGC AAAGGTGAAA 
TCCTACAAGA GACAAGCTGA AGAAGCGGAG GAACAATCCA ACGTCAACCT CTCCAAATTC 
CGCAGGATCC AGCACGAGCT GGAGGAGGCC GAGGAACGGG CTGACATTGC TGAGTCCCAG 
GTCAACAAGC TGAGGGTGAA GAGCAGGGAG GTTCACACAA AAATCATAAG TGAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880164.1
CDS	268..6087
Translation	MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEVTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIEILGFTSDERVSIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQNLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNAVGALAKAVYDKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKPEAHFSLIHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLALLFVGATGAEAEAGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQAMCRGFLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADSLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKIRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSGLQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMETVSKAKGNLEKMCRALEDQLSEIKTKEEEQQRLINDLTAQRARLQTESGEYSRQLDEKDTLVSQLSRGKQAFTQQIEELKRQLEEEIKAKSALAHALQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILAEWKQKCEETHAELEASQKESRSLSTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKSELQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQMKRNHIRIVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQAILKDTQLHLDDALRSQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNVEAVKGLRKHERKVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNVNLSKFRRIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE

Target ORF information:

RefSeq Version	XM_017024675.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 1 (MYH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024675.1

ORF Insert Sequence:

ATGAGTTCCG ACTCTGAGAT GGCCATTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAGGGAGC GAATTGAAGC CCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTGGACCCTA AGGAGTCCTT TGTGAAAGCA ACAGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCTAAGA CCGAAGCTGG AGCTACTGTA ACAGTGAAAG ATGACCAAGT CTTCCCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAC ATGGCCATGA TGACTCATCT ACACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGCTAC GCAGCCTGGA TGATCTACAC CTACTCAGGC 
TTGTTCTGTG TCACTGTCAA CCCCTACAAG TGGTTGCCAG TGTATAATGC AGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGCGCAG GGAAGACTGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGGG AGAAGAAGAA GGAAGAAGTT ACTTCTGGCA AAATGCAGGG GACTCTGGAA 
GATCAAATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTCGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTTAC TTTCCAGCTA 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGATCTA 
ATTGAAATGC TCCTGATCAC CACCAACCCA TACGATTATG CCTTCGTCAG TCAAGGGGAG 
ATCACAGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CTACAGATAG TGCCATTGAA 
ATTCTGGGCT TTACTTCAGA TGAAAGAGTG TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAGGC AGCCTATCTC CAAAATCTGA ACTCTGCAGA TCTGCTCAAA 
GCCCTCTGCT ACCCTAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG TCAAACTGTG 
CAGCAGGTGT ACAATGCAGT GGGTGCTCTG GCCAAAGCTG TCTACGATAA GATGTTCTTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATT 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAG TGGACGTTCA TTGACTTTGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAACATCTT 
GGAAAATCCA ATAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGCCTGA GGCCCACTTC 
TCTTTGATTC ACTATGCTGG CACCGTGGAC TACAACATTG CCGGCTGGCT TGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTG 
GCTCTCCTCT TTGTTGGGGC AACGGGAGCG GAAGCAGAGG CTGGCGGTGG AAAGAAAGGT 
GGTAAGAAGA AGGGTTCTTC TTTCCAGACT GTGTCTGCTC TCTTCAGGGA GAATTTGAAT 
AAGCTGATGA CCAACTTGAG GAGCACTCAC CCCCACTTTG TGCGGTGCAT CATCCCCAAT 
GAAACTAAAA CTCCTGGTGC CATGGAGCAT GAGCTTGTCC TGCATCAGCT GAGGTGTAAC 
GGTGTGCTGG AAGGCATCCG CATCTGCAGG AAAGGCTTCC CAAGCAGAAT CCTTTATGCA 
GACTTCAAAC AGAGATACAA GGTGTTAAAT GCAAGTGCTA TCCCTGAAGG ACAATTCATC 
GATAGCAAGA AGGCTTCAGA GAAGCTCCTG GGGTCCATTG ACATTGACCA CACCCAGTAT 
AAATTTGGTC ACACCAAGGT CTTTTTCAAA GCTGGTCTTC TGGGGCTCCT AGAGGAGATG 
CGAGATGAGA AGCTGGCCCA GCTGATTACC CGAACCCAGG CCATGTGCAG AGGGTTCTTG 
GCAAGAGTGG AGTACCAGAA AATGGTGGAA AGAAGAGAGT CCATCTTCTG CATCCAGTAC 
AATGTCCGTG CCTTCATGAA TGTGAAGCAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAACCCCTCC TCAAAAGTGC AGAGACAGAG AAGGAGATGG CCAACATGAA GGAAGAATTT 
GAGAAAACCA AAGAAGAGCT GGCTAAGACC GAGGCAAAAA GGAAAGAGCT GGAAGAAAAA 
ATGGTTACTC TGATGCAAGA AAAAAATGAC TTGCAACTCC AGGTTCAAGC TGAAGCTGAC 
AGCTTGGCTG ATGCAGAGGA AAGGTGTGAC CAGCTAATCA AAACCAAAAT CCAGCTAGAA 
GCCAAAATCA AAGAGGTGAC TGAGAGAGCT GAGGATGAGG AAGAGATCAA TGCTGAGCTG 
ACAGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAAC TCAAGAAAGA CATTGATGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAACATG CCACAGAAAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC GGGTCTGGAT GAAACCATTG CTAAGCTGAC CAAGGAGAAG 
AAGGCTCTCC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGGCAGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC TAAAATCAAA CTTGAACAAC AAGTGGATGA TCTTGAAGGA 
TCTTTGGAAC AAGAAAAGAA AATCCGGATG GATCTAGAAA GAGCAAAGAG AAAACTAGAG 
GGAGACCTAA AATTGGCTCA AGAATCCACA ATGGATATAG AAAATGACAA ACAACAACTT 
GATGAAAAGC TTAAAAAGAA AGAGTTTGAA ATGAGCGGTC TGCAAAGCAA GATTGAAGAT 
GAACAAGCCC TTGGTATGCA GCTGCAGAAG AAAATCAAGG AGTTACAAGC CCGCATTGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCCGGG CCAAAGCAGA GAAGCAGCGC 
TCTGATCTCT CCCGGGAGCT GGAGGAGATC AGCGAGAGGC TGGAAGAAGC CGGTGGGGCC 
ACCTCAGCCC AGATTGAGAT GAACAAGAAG CGGGAGGCTG AGTTCCAGAA AATGCGCAGG 
GACCTGGAGG AGGCCACCCT ACAGCATGAA GCCACGGCGG CCACCCTGAG GAAGAAGCAT 
GCAGATAGTG TGGCCGAGCT TGGGGAGCAG ATTGACAACC TGCAGCGAGT GAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GATGAAGATG GAGATCGATG ACCTTGCTAG TAACATGGAG 
ACTGTCTCCA AAGCCAAGGG AAACCTTGAA AAGATGTGCC GCGCTCTAGA AGATCAACTG 
AGTGAAATTA AGACCAAGGA AGAGGAGCAG CAGCGGCTGA TCAATGACCT CACAGCACAG 
AGAGCGCGCC TGCAAACAGA ATCAGGTGAA TATTCACGCC AGCTAGATGA AAAGGACACA 
CTAGTTTCAC AGCTCTCGAG GGGCAAACAA GCCTTTACAC AACAGATTGA GGAACTGAAA 
AGGCAACTTG AAGAGGAGAT AAAGGCCAAG AGTGCCCTGG CACATGCCCT GCAGTCCTCC 
CGCCATGACT GTGACCTGCT GCGGGAACAG TATGAGGAGG AGCAGGAAGC CAAGGCCGAG 
CTACAGAGAG CAATGTCCAA GGCCAACAGT GAGGTTGCCC AGTGGAGGAC CAAATATGAG 
ACAGATGCCA TCCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT GGCTCAGCGT 
CTGCAGGATG CTGAGGAACA TGTAGAAGCT GTGAATGCCA AATGTGCTTC CCTTGAGAAG 
ACGAAGCAGA GGCTCCAGAA TGAAGTTGAG GACCTCATGA TTGATGTTGA GAGGACAAAT 
GCTGCCTGTG CCGCCCTGGA CAAAAAGCAA AGGAACTTTG ATAAGATCCT GGCAGAATGG 
AAACAGAAGT GTGAAGAAAC TCATGCTGAA CTTGAAGCTT CTCAAAAGGA ATCCCGCTCA 
CTCAGCACAG AACTATTTAA GATTAAGAAT GCTTATGAGG AATCTTTAGA CCAACTTGAA 
ACCTTGAAAC GGGAAAATAA GAATTTGCAA CAGGAGATTT CTGATCTCAC TGAACAGATT 
GCAGAAGGAG GAAAGCGCAT CCATGAACTG GAAAAAATAA AGAAGCAAGT TGAGCAAGAA 
AAGTCTGAAC TTCAGGCTGC CTTAGAGGAG GCAGAGGCAT CTCTTGAACA TGAAGAGGGA 
AAGATCCTGC GCATCCAGCT TGAGTTGAAC CAAGTCAAGT CTGAGGTTGA TAGGAAAATT 
GCTGAAAAAG ATGAGGAAAT TGACCAGATG AAGAGAAACC ACATTAGAAT CGTGGAGTCC 
ATGCAGAGCA CACTGGATGC TGAGATCAGG AGCAGGAATG ATGCCATTAG GCTCAAGAAG 
AAGATGGAGG GAGACCTCAA TGAAATGGAA ATCCAGCTGA ACCATGCCAA CCGCATGGCT 
GCTGAGGCCC TGAGGAACTA TAGGAACACC CAAGCCATCC TCAAGGATAC CCAGCTCCAC 
CTGGATGATG CTCTCCGGAG CCAGGAGGAC CTGAAGGAAC AGCTGGCTAT GGTGGAGCGC 
AGAGCCAACC TGCTGCAGGC TGAGATCGAG GAGCTGCGGG CCACTCTGGA ACAGACAGAG 
AGGAGCAGGA AAATCGCAGA ACAGGAGCTC CTGGATGCCA GTGAGCGTGT TCAGCTCCTG 
CACACCCAGA ACACCAGCCT GATCAACACC AAGAAGAAGC TGGAGACAGA CATTTCCCAA 
ATCCAGGGAG AGATGGAAGA CATCATCCAG GAAGCCCGCA ATGCAGAAGA GAAGGCCAAG 
AAGGCCATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAACA GGACACCAGC 
GCCCATCTGG AGCGGATGAA GAAGAACCTG GAACAGACGG TGAAGGACCT GCAGCATCGT 
CTGGATGAGG CTGAGCAGCT GGCCCTGAAG GGTGGGAAGA AGCAGATCCA GAAACTGGAG 
GCCAGGGTTC GTGAACTTGA AGGTGAAGTT GAAAGTGAAC AGAAGCGCAA TGTTGAAGCT 
GTCAAGGGTC TACGCAAACA TGAGAGAAAA GTGAAGGAAC TCACTTACCA AACTGAGGAA 
GACCGCAAGA ATATTCTCAG GCTCCAGGAC CTGGTGGACA AACTGCAAGC AAAGGTGAAA 
TCCTACAAGA GACAAGCTGA AGAAGCGGAG GAACAATCCA ACGTCAACCT CTCCAAATTC 
CGCAGGATCC AGCACGAGCT GGAGGAGGCC GAGGAACGGG CTGACATTGC TGAGTCCCAG 
GTCAACAAGC TGAGGGTGAA GAGCAGGGAG GTTCACACAA AAATCATAAG TGAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transition of myosin heavy chain isoforms in human laryngeal abductors following denervation.
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery272(10)2915-23(2015 Oct)
Qiu X,Chen D,Li M,Gao Y,Liu F,Zheng H,Chen S

Molecular determinants of force production in human skeletal muscle fibers: effects of myosin isoform expression and cross-sectional area.
American journal of physiology. Cell physiology308(6)C473-84(2015 Mar)
Miller MS,Bedrin NG,Ades PA,Palmer BM,Toth MJ

Wearing of complete dentures reduces slow fibre and enhances hybrid fibre fraction in masseter muscle.
Journal of oral rehabilitation39(8)608-14(2012 Aug)
Cvetko E,Karen P,Er?en I

Myosin heavy chain composition of the human genioglossus muscle.
Journal of speech, language, and hearing research : JSLHR55(2)609-25(2012 Apr)
Daugherty M,Luo Q,Sokoloff AJ

The expression of myosin heavy chain (MHC) genes in human skeletal muscle is related to metabolic characteristics involved in the pathogenesis of type 2 diabetes.
Molecular genetics and metabolism103(3)275-81(2011 Jul)
Olsson AH,R?nn T,Elgzyri T,Hansson O,Eriksson KF,Groop L,Vaag A,Poulsen P,Ling C

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MYH1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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