MTHFD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTHFD1
Entrez Gene ID	4522
Full Name	methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1
Synonyms	CIMAH,MTHFC,MTHFD
General protein information	Preferred Names methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 Names C-1-tetrahydrofolate synthase, cytoplasmic 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase C1-THF synthase cytoplasmic C-1-tetrahydrofolate synthase methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q23.3
Summary	This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008].
Disorder MIM:	172460
Disorder Html:	{Neural tube defects, folate-sensitive, susceptibility to}, 601634 (3); Combined immunodeficiency and megaloblastic anemia with or without hyperhomocysteinemia, 617780 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005956.4	NP_005947.3	C-1-tetrahydrofolate synthase, cytoplasmic isoform 1
NM_005956.3	NP_005947.3	C-1-tetrahydrofolate synthase, cytoplasmic
NM_001364837.1	NP_001351766.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MTHFD1	NP_001076946.1
Danio rerio (zebrafish)	mthfd1b	NP_955823.1
Arabidopsis thaliana (thale cress)	THFS	NP_564571.1
Pan troglodytes (chimpanzee)	MTHFD1	XP_510001.3
Gallus gallus (chicken)	MTHFD1	NP_001034392.1
Oryza sativa (rice)	Os09g0446800	NP_001063310.1
Xenopus tropicalis (tropical clawed frog)	mthfd1	NP_001008007.1
Homo sapiens (human)	MTHFD1	NP_005947.3
Drosophila melanogaster (fruit fly)	pug	NP_731489.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002830	XP_312083.5
Caenorhabditis elegans (roundworm)	K07E3.4	NP_509360.1
Schizosaccharomyces pombe (fission yeast)	SPBC839.16	NP_595256.1
Neurospora crassa	NCU03877	XP_956550.1
	MTHFD1	XP_001101708.2
Rattus norvegicus (Norway rat)	Mthfd1	NP_071953.1
Kluyveromyces lactis	KLLA0E16545g	XP_454695.1
Eremothecium gossypii	AGOS_ACL121C	NP_983283.2
Magnaporthe oryzae (rice blast fungus)	MGG_01014	XP_003717930.1
Canis lupus familiaris (dog)	MTHFD1	XP_537476.2
Mus musculus (house mouse)	Mthfd1	NP_620084.2
Saccharomyces cerevisiae (baker's yeast)	ADE3	NP_011720.3

Gene Ontology

The following MTHFD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTHFD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22391	NM_005956.4 Latest version!	Homo sapiens methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu107572	NM_001364837.1 Latest version!	Homo sapiens methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu22391	NM_005956.3	Homo sapiens methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTHFD1 ( NM_005956.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005947.3 Homo sapiens methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22391
Clone ID Related Accession (Same CDS sequence)	NM_005956.4 , NM_005956.3
Accession Version	NM_005956.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ959308.1, J04031.1 and AL122035.6. This sequence is a reference standard in the RefSeqGene project. On Jul 5, 2018 this sequence version replaced NM_005956.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.225324.1, SRR1803615.99280.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000652337.1/ ENSP00000498336.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGCCAG CAGAAATCCT GAACGGGAAG GAGATCTCCG CGCAAATAAG GGCGAGACTG 
AAAAATCAAG TCACTCAGTT GAAGGAGCAA GTACCTGGTT TCACACCACG CCTGGCAATA 
TTACAGGTTG GCAACAGAGA TGATTCCAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATCAAAGC CACTCACATT AAGTTACCAA GAACAACCAC AGAATCTGAG 
GTGATGAAGT ACATTACATC TTTGAATGAA GACTCTACTG TACATGGGTT CTTAGTGCAG 
CTACCTTTAG ATTCAGAGAA TTCCATTAAC ACTGAAGAAG TGATCAATGC TATTGCACCC 
GAGAAGGATG TGGATGGATT GACTAGCATC AATGCTGGGA AACTTGCTAG AGGTGACCTC 
AATGACTGTT TCATTCCTTG TACGCCTAAG GGATGCTTGG AACTCATCAA AGAGACAGGG 
GTGCCGATTG CCGGAAGGCA TGCTGTGGTG GTTGGGCGCA GTAAAATAGT TGGGGCCCCG 
ATGCATGACT TGCTTCTGTG GAACAATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
CATCTGGATG AGGAGGTAAA TAAAGGTGAC ATCCTGGTGG TTGCAACTGG TCAGCCTGAA 
ATGGTTAAAG GGGAGTGGAT CAAACCTGGG GCAATAGTCA TCGACTGTGG AATCAATTAT 
GTCCCAGATG ATAAAAAACC AAATGGGAGA AAAGTTGTGG GTGATGTGGC ATACGACGAG 
GCCAAAGAGA GGGCGAGCTT CATCACTCCT GTTCCTGGCG GCGTAGGGCC CATGACAGTT 
GCAATGCTCA TGCAGAGCAC AGTAGAGAGT GCCAAGCGTT TCCTGGAGAA ATTTAAGCCA 
GGAAAGTGGA TGATTCAGTA TAACAACCTT AACCTCAAGA CACCTGTTCC AAGTGACATT 
GATATATCAC GATCTTGTAA ACCGAAGCCC ATTGGTAAGC TGGCTCGAGA AATTGGTCTG 
CTGTCTGAAG AGGTAGAATT ATATGGTGAA ACAAAGGCCA AAGTTCTGCT GTCAGCACTA 
GAACGCCTGA AGCACCGGCC TGATGGGAAA TACGTGGTGG TGACTGGAAT AACTCCAACA 
CCCCTGGGAG AAGGGAAAAG CACAACTACA ATCGGGCTAG TGCAAGCCCT TGGTGCCCAT 
CTCTACCAGA ATGTCTTTGC GTGTGTGCGA CAGCCTTCTC AGGGCCCCAC CTTTGGAATA 
AAAGGTGGCG CTGCAGGAGG CGGCTACTCC CAGGTCATTC CTATGGAAGA GTTTAATCTC 
CACCTCACAG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTCGTTGC TGCGGCCATT 
GATGCTCGGA TATTTCATGA ACTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTG 
CCATCAGTAA ATGGAGTGAG AAGGTTCTCT GACATCCAAA TCCGAAGGTT AAAGAGACTA 
GGCATTGAAA AGACTGACCC TACCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGACATTG ATCCAGAAAC CATAACTTGG CAAAGAGTGT TGGATACCAA TGATAGATTC 
CTGAGGAAGA TCACGATTGG ACAGGCTCCA ACGGAGAAGG GTCACACACG GACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATTATG GCTGTCCTGG CTCTCACCAC TTCTCTAGAA 
GACATGAGAG AGAGACTGGG CAAAATGGTG GTGGCATCCA GTAAGAAAGG AGAGCCCGTC 
AGTGCCGAAG ATCTGGGGGT GAGTGGTGCA CTGACAGTGC TTATGAAGGA CGCAATCAAG 
CCCAATCTCA TGCAGACACT GGAGGGCACT CCAGTGTTTG TCCATGCTGG CCCGTTTGCC 
AACATCGCAC ATGGCAATTC CTCCATCATT GCAGACCGGA TCGCACTCAA GCTTGTTGGC 
CCAGAAGGGT TTGTAGTGAC GGAAGCAGGA TTTGGAGCAG ACATTGGAAT GGAAAAGTTT 
TTTAACATCA AATGCCGGTA TTCCGGCCTC TGCCCCCACG TGGTGGTGCT TGTTGCCACT 
GTCAGGGCTC TCAAGATGCA CGGGGGCGGC CCCACGGTCA CTGCTGGACT GCCTCTTCCC 
AAGGCTTACA TACAGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTGAAGAAA 
CAAATTGAAA ATGCCAGAAT GTTTGGAATT CCAGTAGTAG TGGCCGTGAA TGCATTCAAG 
ACGGATACAG AGTCTGAGCT GGACCTCATC AGCCGCCTTT CCAGAGAACA TGGGGCTTTT 
GATGCCGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAAGG GTGCCTTAGC CCTGGCTCAG 
GCCGTCCAGA GAGCAGCACA AGCACCCAGC AGCTTCCAGC TCCTTTATGA CCTCAAGCTC 
CCAGTTGAGG ATAAAATCAG GATCATTGCA CAGAAGATCT ATGGAGCAGA TGACATTGAA 
TTACTTCCCG AAGCTCAACA CAAAGCTGAA GTCTACACGA AGCAGGGCTT TGGGAATCTC 
CCCATCTGCA TGGCTAAAAC ACACTTGTCT TTGTCTCACA ACCCAGAGCA AAAAGGTGTC 
CCTACAGGCT TCATTCTGCC CATTCGCGAC ATCCGCGCCA GCGTTGGGGC TGGTTTTCTG 
TACCCCTTAG TAGGAACGAT GAGCACAATG CCTGGACTCC CCACCCGGCC CTGTTTTTAT 
GATATTGATT TGGACCCTGA AACAGAACAG GTGAATGGAT TATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005947.3
CDS	76..2883
Translation	MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTAHLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGETKAKVLLSALERLKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLYQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFSDIQIRRLKRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Target ORF information:

RefSeq Version	NM_005956.4
Organism	Homo sapiens(human)
Definition	Homo sapiens methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005956.4

ORF Insert Sequence:

ATGGCGCCAG CAGAAATCCT GAACGGGAAG GAGATCTCCG CGCAAATAAG GGCGAGACTG 
AAAAATCAAG TCACTCAGTT GAAGGAGCAA GTACCTGGTT TCACACCACG CCTGGCAATA 
TTACAGGTTG GCAACAGAGA TGATTCCAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATCAAAGC CACTCACATT AAGTTACCAA GAACAACCAC AGAATCTGAG 
GTGATGAAGT ACATTACATC TTTGAATGAA GACTCTACTG TACATGGGTT CTTAGTGCAG 
CTACCTTTAG ATTCAGAGAA TTCCATTAAC ACTGAAGAAG TGATCAATGC TATTGCACCC 
GAGAAGGATG TGGATGGATT GACTAGCATC AATGCTGGGA AACTTGCTAG AGGTGACCTC 
AATGACTGTT TCATTCCTTG TACGCCTAAG GGATGCTTGG AACTCATCAA AGAGACAGGG 
GTGCCGATTG CCGGAAGGCA TGCTGTGGTG GTTGGGCGCA GTAAAATAGT TGGGGCCCCG 
ATGCATGACT TGCTTCTGTG GAACAATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
CATCTGGATG AGGAGGTAAA TAAAGGTGAC ATCCTGGTGG TTGCAACTGG TCAGCCTGAA 
ATGGTTAAAG GGGAGTGGAT CAAACCTGGG GCAATAGTCA TCGACTGTGG AATCAATTAT 
GTCCCAGATG ATAAAAAACC AAATGGGAGA AAAGTTGTGG GTGATGTGGC ATACGACGAG 
GCCAAAGAGA GGGCGAGCTT CATCACTCCT GTTCCTGGCG GCGTAGGGCC CATGACAGTT 
GCAATGCTCA TGCAGAGCAC AGTAGAGAGT GCCAAGCGTT TCCTGGAGAA ATTTAAGCCA 
GGAAAGTGGA TGATTCAGTA TAACAACCTT AACCTCAAGA CACCTGTTCC AAGTGACATT 
GATATATCAC GATCTTGTAA ACCGAAGCCC ATTGGTAAGC TGGCTCGAGA AATTGGTCTG 
CTGTCTGAAG AGGTAGAATT ATATGGTGAA ACAAAGGCCA AAGTTCTGCT GTCAGCACTA 
GAACGCCTGA AGCACCGGCC TGATGGGAAA TACGTGGTGG TGACTGGAAT AACTCCAACA 
CCCCTGGGAG AAGGGAAAAG CACAACTACA ATCGGGCTAG TGCAAGCCCT TGGTGCCCAT 
CTCTACCAGA ATGTCTTTGC GTGTGTGCGA CAGCCTTCTC AGGGCCCCAC CTTTGGAATA 
AAAGGTGGCG CTGCAGGAGG CGGCTACTCC CAGGTCATTC CTATGGAAGA GTTTAATCTC 
CACCTCACAG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTCGTTGC TGCGGCCATT 
GATGCTCGGA TATTTCATGA ACTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTG 
CCATCAGTAA ATGGAGTGAG AAGGTTCTCT GACATCCAAA TCCGAAGGTT AAAGAGACTA 
GGCATTGAAA AGACTGACCC TACCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGACATTG ATCCAGAAAC CATAACTTGG CAAAGAGTGT TGGATACCAA TGATAGATTC 
CTGAGGAAGA TCACGATTGG ACAGGCTCCA ACGGAGAAGG GTCACACACG GACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATTATG GCTGTCCTGG CTCTCACCAC TTCTCTAGAA 
GACATGAGAG AGAGACTGGG CAAAATGGTG GTGGCATCCA GTAAGAAAGG AGAGCCCGTC 
AGTGCCGAAG ATCTGGGGGT GAGTGGTGCA CTGACAGTGC TTATGAAGGA CGCAATCAAG 
CCCAATCTCA TGCAGACACT GGAGGGCACT CCAGTGTTTG TCCATGCTGG CCCGTTTGCC 
AACATCGCAC ATGGCAATTC CTCCATCATT GCAGACCGGA TCGCACTCAA GCTTGTTGGC 
CCAGAAGGGT TTGTAGTGAC GGAAGCAGGA TTTGGAGCAG ACATTGGAAT GGAAAAGTTT 
TTTAACATCA AATGCCGGTA TTCCGGCCTC TGCCCCCACG TGGTGGTGCT TGTTGCCACT 
GTCAGGGCTC TCAAGATGCA CGGGGGCGGC CCCACGGTCA CTGCTGGACT GCCTCTTCCC 
AAGGCTTACA TACAGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTGAAGAAA 
CAAATTGAAA ATGCCAGAAT GTTTGGAATT CCAGTAGTAG TGGCCGTGAA TGCATTCAAG 
ACGGATACAG AGTCTGAGCT GGACCTCATC AGCCGCCTTT CCAGAGAACA TGGGGCTTTT 
GATGCCGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAAGG GTGCCTTAGC CCTGGCTCAG 
GCCGTCCAGA GAGCAGCACA AGCACCCAGC AGCTTCCAGC TCCTTTATGA CCTCAAGCTC 
CCAGTTGAGG ATAAAATCAG GATCATTGCA CAGAAGATCT ATGGAGCAGA TGACATTGAA 
TTACTTCCCG AAGCTCAACA CAAAGCTGAA GTCTACACGA AGCAGGGCTT TGGGAATCTC 
CCCATCTGCA TGGCTAAAAC ACACTTGTCT TTGTCTCACA ACCCAGAGCA AAAAGGTGTC 
CCTACAGGCT TCATTCTGCC CATTCGCGAC ATCCGCGCCA GCGTTGGGGC TGGTTTTCTG 
TACCCCTTAG TAGGAACGAT GAGCACAATG CCTGGACTCC CCACCCGGCC CTGTTTTTAT 
GATATTGATT TGGACCCTGA AACAGAACAG GTGAATGGAT TATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1 ( NM_005956.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005947.3 Homo sapiens methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), mRNA.

Price & Availability↑

CloneID	OHu22391
Clone ID Related Accession (Same CDS sequence)	NM_005956.4 , NM_005956.3
Accession Version	NM_005956.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-01
Organism	Homo sapiens(human)
Product	C-1-tetrahydrofolate synthase, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC368835.1, BQ959308.1, J04031.1, AL122035.6 and CA446032.1. This sequence is a reference standard in the RefSeqGene project. On Jan 29, 2009 this sequence version replaced NM_005956.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.7007.1, SRR1803617.79813.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:3053686) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGCCAG CAGAAATCCT GAACGGGAAG GAGATCTCCG CGCAAATAAG GGCGAGACTG 
AAAAATCAAG TCACTCAGTT GAAGGAGCAA GTACCTGGTT TCACACCACG CCTGGCAATA 
TTACAGGTTG GCAACAGAGA TGATTCCAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATCAAAGC CACTCACATT AAGTTACCAA GAACAACCAC AGAATCTGAG 
GTGATGAAGT ACATTACATC TTTGAATGAA GACTCTACTG TACATGGGTT CTTAGTGCAG 
CTACCTTTAG ATTCAGAGAA TTCCATTAAC ACTGAAGAAG TGATCAATGC TATTGCACCC 
GAGAAGGATG TGGATGGATT GACTAGCATC AATGCTGGGA AACTTGCTAG AGGTGACCTC 
AATGACTGTT TCATTCCTTG TACGCCTAAG GGATGCTTGG AACTCATCAA AGAGACAGGG 
GTGCCGATTG CCGGAAGGCA TGCTGTGGTG GTTGGGCGCA GTAAAATAGT TGGGGCCCCG 
ATGCATGACT TGCTTCTGTG GAACAATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
CATCTGGATG AGGAGGTAAA TAAAGGTGAC ATCCTGGTGG TTGCAACTGG TCAGCCTGAA 
ATGGTTAAAG GGGAGTGGAT CAAACCTGGG GCAATAGTCA TCGACTGTGG AATCAATTAT 
GTCCCAGATG ATAAAAAACC AAATGGGAGA AAAGTTGTGG GTGATGTGGC ATACGACGAG 
GCCAAAGAGA GGGCGAGCTT CATCACTCCT GTTCCTGGCG GCGTAGGGCC CATGACAGTT 
GCAATGCTCA TGCAGAGCAC AGTAGAGAGT GCCAAGCGTT TCCTGGAGAA ATTTAAGCCA 
GGAAAGTGGA TGATTCAGTA TAACAACCTT AACCTCAAGA CACCTGTTCC AAGTGACATT 
GATATATCAC GATCTTGTAA ACCGAAGCCC ATTGGTAAGC TGGCTCGAGA AATTGGTCTG 
CTGTCTGAAG AGGTAGAATT ATATGGTGAA ACAAAGGCCA AAGTTCTGCT GTCAGCACTA 
GAACGCCTGA AGCACCGGCC TGATGGGAAA TACGTGGTGG TGACTGGAAT AACTCCAACA 
CCCCTGGGAG AAGGGAAAAG CACAACTACA ATCGGGCTAG TGCAAGCCCT TGGTGCCCAT 
CTCTACCAGA ATGTCTTTGC GTGTGTGCGA CAGCCTTCTC AGGGCCCCAC CTTTGGAATA 
AAAGGTGGCG CTGCAGGAGG CGGCTACTCC CAGGTCATTC CTATGGAAGA GTTTAATCTC 
CACCTCACAG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTCGTTGC TGCGGCCATT 
GATGCTCGGA TATTTCATGA ACTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTG 
CCATCAGTAA ATGGAGTGAG AAGGTTCTCT GACATCCAAA TCCGAAGGTT AAAGAGACTA 
GGCATTGAAA AGACTGACCC TACCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGACATTG ATCCAGAAAC CATAACTTGG CAAAGAGTGT TGGATACCAA TGATAGATTC 
CTGAGGAAGA TCACGATTGG ACAGGCTCCA ACGGAGAAGG GTCACACACG GACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATTATG GCTGTCCTGG CTCTCACCAC TTCTCTAGAA 
GACATGAGAG AGAGACTGGG CAAAATGGTG GTGGCATCCA GTAAGAAAGG AGAGCCCGTC 
AGTGCCGAAG ATCTGGGGGT GAGTGGTGCA CTGACAGTGC TTATGAAGGA CGCAATCAAG 
CCCAATCTCA TGCAGACACT GGAGGGCACT CCAGTGTTTG TCCATGCTGG CCCGTTTGCC 
AACATCGCAC ATGGCAATTC CTCCATCATT GCAGACCGGA TCGCACTCAA GCTTGTTGGC 
CCAGAAGGGT TTGTAGTGAC GGAAGCAGGA TTTGGAGCAG ACATTGGAAT GGAAAAGTTT 
TTTAACATCA AATGCCGGTA TTCCGGCCTC TGCCCCCACG TGGTGGTGCT TGTTGCCACT 
GTCAGGGCTC TCAAGATGCA CGGGGGCGGC CCCACGGTCA CTGCTGGACT GCCTCTTCCC 
AAGGCTTACA TACAGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTGAAGAAA 
CAAATTGAAA ATGCCAGAAT GTTTGGAATT CCAGTAGTAG TGGCCGTGAA TGCATTCAAG 
ACGGATACAG AGTCTGAGCT GGACCTCATC AGCCGCCTTT CCAGAGAACA TGGGGCTTTT 
GATGCCGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAAGG GTGCCTTAGC CCTGGCTCAG 
GCCGTCCAGA GAGCAGCACA AGCACCCAGC AGCTTCCAGC TCCTTTATGA CCTCAAGCTC 
CCAGTTGAGG ATAAAATCAG GATCATTGCA CAGAAGATCT ATGGAGCAGA TGACATTGAA 
TTACTTCCCG AAGCTCAACA CAAAGCTGAA GTCTACACGA AGCAGGGCTT TGGGAATCTC 
CCCATCTGCA TGGCTAAAAC ACACTTGTCT TTGTCTCACA ACCCAGAGCA AAAAGGTGTC 
CCTACAGGCT TCATTCTGCC CATTCGCGAC ATCCGCGCCA GCGTTGGGGC TGGTTTTCTG 
TACCCCTTAG TAGGAACGAT GAGCACAATG CCTGGACTCC CCACCCGGCC CTGTTTTTAT 
GATATTGATT TGGACCCTGA AACAGAACAG GTGAATGGAT TATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005947.3
CDS	388..3195
Translation	MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTAHLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGETKAKVLLSALERLKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLYQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFSDIQIRRLKRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

Target ORF information:

RefSeq Version	NM_005956.3
Organism	Homo sapiens(human)
Definition	Homo sapiens methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005956.3

ORF Insert Sequence:

ATGGCGCCAG CAGAAATCCT GAACGGGAAG GAGATCTCCG CGCAAATAAG GGCGAGACTG 
AAAAATCAAG TCACTCAGTT GAAGGAGCAA GTACCTGGTT TCACACCACG CCTGGCAATA 
TTACAGGTTG GCAACAGAGA TGATTCCAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATCAAAGC CACTCACATT AAGTTACCAA GAACAACCAC AGAATCTGAG 
GTGATGAAGT ACATTACATC TTTGAATGAA GACTCTACTG TACATGGGTT CTTAGTGCAG 
CTACCTTTAG ATTCAGAGAA TTCCATTAAC ACTGAAGAAG TGATCAATGC TATTGCACCC 
GAGAAGGATG TGGATGGATT GACTAGCATC AATGCTGGGA AACTTGCTAG AGGTGACCTC 
AATGACTGTT TCATTCCTTG TACGCCTAAG GGATGCTTGG AACTCATCAA AGAGACAGGG 
GTGCCGATTG CCGGAAGGCA TGCTGTGGTG GTTGGGCGCA GTAAAATAGT TGGGGCCCCG 
ATGCATGACT TGCTTCTGTG GAACAATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
CATCTGGATG AGGAGGTAAA TAAAGGTGAC ATCCTGGTGG TTGCAACTGG TCAGCCTGAA 
ATGGTTAAAG GGGAGTGGAT CAAACCTGGG GCAATAGTCA TCGACTGTGG AATCAATTAT 
GTCCCAGATG ATAAAAAACC AAATGGGAGA AAAGTTGTGG GTGATGTGGC ATACGACGAG 
GCCAAAGAGA GGGCGAGCTT CATCACTCCT GTTCCTGGCG GCGTAGGGCC CATGACAGTT 
GCAATGCTCA TGCAGAGCAC AGTAGAGAGT GCCAAGCGTT TCCTGGAGAA ATTTAAGCCA 
GGAAAGTGGA TGATTCAGTA TAACAACCTT AACCTCAAGA CACCTGTTCC AAGTGACATT 
GATATATCAC GATCTTGTAA ACCGAAGCCC ATTGGTAAGC TGGCTCGAGA AATTGGTCTG 
CTGTCTGAAG AGGTAGAATT ATATGGTGAA ACAAAGGCCA AAGTTCTGCT GTCAGCACTA 
GAACGCCTGA AGCACCGGCC TGATGGGAAA TACGTGGTGG TGACTGGAAT AACTCCAACA 
CCCCTGGGAG AAGGGAAAAG CACAACTACA ATCGGGCTAG TGCAAGCCCT TGGTGCCCAT 
CTCTACCAGA ATGTCTTTGC GTGTGTGCGA CAGCCTTCTC AGGGCCCCAC CTTTGGAATA 
AAAGGTGGCG CTGCAGGAGG CGGCTACTCC CAGGTCATTC CTATGGAAGA GTTTAATCTC 
CACCTCACAG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTCGTTGC TGCGGCCATT 
GATGCTCGGA TATTTCATGA ACTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTG 
CCATCAGTAA ATGGAGTGAG AAGGTTCTCT GACATCCAAA TCCGAAGGTT AAAGAGACTA 
GGCATTGAAA AGACTGACCC TACCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGACATTG ATCCAGAAAC CATAACTTGG CAAAGAGTGT TGGATACCAA TGATAGATTC 
CTGAGGAAGA TCACGATTGG ACAGGCTCCA ACGGAGAAGG GTCACACACG GACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATTATG GCTGTCCTGG CTCTCACCAC TTCTCTAGAA 
GACATGAGAG AGAGACTGGG CAAAATGGTG GTGGCATCCA GTAAGAAAGG AGAGCCCGTC 
AGTGCCGAAG ATCTGGGGGT GAGTGGTGCA CTGACAGTGC TTATGAAGGA CGCAATCAAG 
CCCAATCTCA TGCAGACACT GGAGGGCACT CCAGTGTTTG TCCATGCTGG CCCGTTTGCC 
AACATCGCAC ATGGCAATTC CTCCATCATT GCAGACCGGA TCGCACTCAA GCTTGTTGGC 
CCAGAAGGGT TTGTAGTGAC GGAAGCAGGA TTTGGAGCAG ACATTGGAAT GGAAAAGTTT 
TTTAACATCA AATGCCGGTA TTCCGGCCTC TGCCCCCACG TGGTGGTGCT TGTTGCCACT 
GTCAGGGCTC TCAAGATGCA CGGGGGCGGC CCCACGGTCA CTGCTGGACT GCCTCTTCCC 
AAGGCTTACA TACAGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTGAAGAAA 
CAAATTGAAA ATGCCAGAAT GTTTGGAATT CCAGTAGTAG TGGCCGTGAA TGCATTCAAG 
ACGGATACAG AGTCTGAGCT GGACCTCATC AGCCGCCTTT CCAGAGAACA TGGGGCTTTT 
GATGCCGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAAGG GTGCCTTAGC CCTGGCTCAG 
GCCGTCCAGA GAGCAGCACA AGCACCCAGC AGCTTCCAGC TCCTTTATGA CCTCAAGCTC 
CCAGTTGAGG ATAAAATCAG GATCATTGCA CAGAAGATCT ATGGAGCAGA TGACATTGAA 
TTACTTCCCG AAGCTCAACA CAAAGCTGAA GTCTACACGA AGCAGGGCTT TGGGAATCTC 
CCCATCTGCA TGGCTAAAAC ACACTTGTCT TTGTCTCACA ACCCAGAGCA AAAAGGTGTC 
CCTACAGGCT TCATTCTGCC CATTCGCGAC ATCCGCGCCA GCGTTGGGGC TGGTTTTCTG 
TACCCCTTAG TAGGAACGAT GAGCACAATG CCTGGACTCC CCACCCGGCC CTGTTTTTAT 
GATATTGATT TGGACCCTGA AACAGAACAG GTGAATGGAT TATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1 ( NM_001364837.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001351766.1 Homo sapiens methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant 2, mRNA.

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CloneID	OHu107572
Clone ID Related Accession (Same CDS sequence)	NM_001364837.1
Accession Version	NM_001364837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302747.1 and AL122035.6. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302747.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCCAG CAGAAATCCT GAACGGGAAG GAGATCTCCG CGCAAATAAG GGCGAGACTG 
AAAAATCAAG TCACTCAGTT GAAGGAGCAA GTACCTGGTT TCACACCACG CCTGGCAATA 
TTACAGGTTG GCAACAGAGA TGATTCCAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATCAAAGC CACTCACATT AAGTTACCAA GAACAACCAC AGAATCTGAG 
GTGATGAAGT ACATTACATC TTTGAATGAA GACTCTACTG TACATGGGTT CTTAGTGCAG 
CTACCTTTAG ATTCAGAGAA TTCCATTAAC ACTGAAGAAG TGATCAATGC TATTGCACCC 
GAGAAGGATG TGGATGGATT GACTAGCATC AATGCTGGGA AACTTGCTAG AGGTGACCTC 
AATGACTGTT TCATTCCTTG TACGCCTAAG GGATGCTTGG AACTCATCAA AGAGACAGGG 
GTGCCGATTG CCGGAAGGCA TGCTGTGGTG GTTGGGCGCA GTAAAATAGT TGGGGCCCCG 
ATGCATGACT TGCTTCTGTG GAACAATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
CATCTGGATG AGGAGGTAAA TAAAGGTGAC ATCCTGGTGG TTGCAACTGG TCAGCCTGAA 
ATGGTTAAAG GGGAGTGGAT CAAACCTGGG GCAATAGTCA TCGACTGTGG AATCAATTAT 
GTCCCAGATG ATAAAAAACC AAATGGGAGA AAAGTTGTGG GTGATGTGGC ATACGACGAG 
GCCAAAGAGA GGGCGAGCTT CATCACTCCT GTTCCTGGCG GCGTAGGGCC CATGACAGTT 
GCAATGCTCA TGCAGAGCAC AGTAGAGAGT GCCAAGCGTT TCCTGGAGAA ATTTAAGCCA 
GGAAAGTGGA TGATTCAGTA TAACAACCTT AACCTCAAGA CACCTGTTCC AAGTGACATT 
GATATATCAC GATCTTGTAA ACCGAAGCCC ATTGGTAAGC TGGCTCGAGA AATTGGTCTG 
CTGTCTGAAG AGGTAGAATT ATATGGTGAA ACAAAGGCCA AAGTTCTGCT GTCAGCACTA 
GAACGCCTGA AGCACCGGCC TGATGGGAAA TACGTGGTGG TGACTGGAAT AACTCCAACA 
CCCCTGGGAG AAGGGAAAAG CACAACTACA ATCGGGCTAG TGCAAGCCCT TGGTGCCCAT 
CTCTACCAGA ATGTCTTTGC GTGTGTGCGA CAGCCTTCTC AGGGCCCCAC CTTTGGAATA 
AAAGGTGGCG CTGCAGGAGG CGGCTACTCC CAGGTCATTC CTATGGAAGA GTTTAATCTC 
CACCTCACAG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTCGTTGC TGCGGCCATT 
GATGCTCGGA TATTTCATGA ACTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTG 
CCATCAGTAA ATGGAGTGAG AAGGTTCTCT GACATCCAAA TCCGAAGGTT AAAGAGACTA 
GGCATTGAAA AGACTGACCC TACCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGACATTG ATCCAGAAAC CATAACTTGG CAAAGAGTGT TGGATACCAA TGATAGATTC 
CTGAGGAAGA TCACGATTGG ACAGGCTCCA ACGGAGAAGG GTCACACACG GACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATTATG GCTGTCCTGG CTCTCACCAC TTCTCTAGAA 
GACATGAGAG AGAGACTGGG CAAAATGGTG GTGGCATCCA GTAAGAAAGG AGAGCCCGTC 
AGTGCCGAAG ATCTGGGGGT GAGTGGTGCA CTGACAGTGC TTATGAAGGA CGCAATCAAG 
CCCAATCTCA TGCAGACACT GGAGGGCACT CCAGTGTTTG TCCATGCTGG CCCGTTTGCC 
AACATCGCAC ATGGCAATTC CTCCATCATT GCAGACCGGA TCGCACTCAA GCTTGTTGGC 
CCAGAAGGGT TTGTAGTGAC GGAAGCAGGA TTTGGAGCAG ACATTGGAAT GGAAAAGTTT 
TTTAACATCA AATGCCGGTA TTCCGGCCTC TGCCCCCACG TGGTGGTGCT TGTTGCCACT 
GTCAGGGCTC TCAAGATGCA CGGGGGCGGC CCCACGGTCA CTGCTGGACT GCCTCTTCCC 
AAGGCTTACA TACAGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTGAAGAAA 
CAAATTGAAA ATGCCAGAAT GTTTGGAATT CCAGTAGTAG TGGCCGTGAA TGCATTCAAG 
ACGGATACAG AGTCTGAGCT GGACCTCATC AGCCGCCTTT CCAGAGAACA TGGGGCTTTT 
GATGCCGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAAGG GTGCCTTAGC CCTGGCTCAG 
GCCGTCCAGA GAGCAGCACA AGCACCCAGC AGCTTCCAGC TCCTTTATGA CCTCAAGCTC 
CCAGTTGAGG ATAAAATCAG GATCATTGCA CAGAAGATCT ATGGAGCAGA TGACATTGAA 
TTACTTCCCG AAGCTCAACA CAAAGCTGAA GTCTACACGA AGCAGGGCTT TGGGAATCTC 
CCCATCTGCA TGGCTAAAAC ACACTTGTCT TTGTCTCACA ACCCAGAGCA AAAAGGTGTC 
CCTACAGGCT TCATTCTGCC CATTCGCGAC ATCCGCGCCA GCGTTGGGGC TGGTTTTCTG 
TACCCCTTAG TAGGAACGAT CACCATCCAT CTTCAAGAAG CTACTTTGAA AGTCTGGCCA 
GTGTCTATTC AGGCCCACTG GGAGTTAGGA AGTATAAGTA AGCCAAGAGA AGTCAGCCCC 
TGCCCAGAAG ATCTGAAACT AATAGTAGGA GTTTCCCCAG AAGTCATTTT CAGCCTTAAT 
TCTCATCATG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351766.1
CDS	76..2970
Translation	MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTAHLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGETKAKVLLSALERLKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLYQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFSDIQIRRLKRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTITIHLQEATLKVWPVSIQAHWELGSISKPREVSPCPEDLKLIVGVSPEVIFSLNSHHV

Target ORF information:

RefSeq Version	NM_001364837.1
Organism	Homo sapiens(human)
Definition	Homo sapiens methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 (MTHFD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364837.1

ORF Insert Sequence:

ATGGCGCCAG CAGAAATCCT GAACGGGAAG GAGATCTCCG CGCAAATAAG GGCGAGACTG 
AAAAATCAAG TCACTCAGTT GAAGGAGCAA GTACCTGGTT TCACACCACG CCTGGCAATA 
TTACAGGTTG GCAACAGAGA TGATTCCAAT CTTTATATAA ATGTGAAGCT GAAGGCTGCT 
GAAGAGATTG GGATCAAAGC CACTCACATT AAGTTACCAA GAACAACCAC AGAATCTGAG 
GTGATGAAGT ACATTACATC TTTGAATGAA GACTCTACTG TACATGGGTT CTTAGTGCAG 
CTACCTTTAG ATTCAGAGAA TTCCATTAAC ACTGAAGAAG TGATCAATGC TATTGCACCC 
GAGAAGGATG TGGATGGATT GACTAGCATC AATGCTGGGA AACTTGCTAG AGGTGACCTC 
AATGACTGTT TCATTCCTTG TACGCCTAAG GGATGCTTGG AACTCATCAA AGAGACAGGG 
GTGCCGATTG CCGGAAGGCA TGCTGTGGTG GTTGGGCGCA GTAAAATAGT TGGGGCCCCG 
ATGCATGACT TGCTTCTGTG GAACAATGCC ACAGTGACCA CCTGCCACTC CAAGACTGCC 
CATCTGGATG AGGAGGTAAA TAAAGGTGAC ATCCTGGTGG TTGCAACTGG TCAGCCTGAA 
ATGGTTAAAG GGGAGTGGAT CAAACCTGGG GCAATAGTCA TCGACTGTGG AATCAATTAT 
GTCCCAGATG ATAAAAAACC AAATGGGAGA AAAGTTGTGG GTGATGTGGC ATACGACGAG 
GCCAAAGAGA GGGCGAGCTT CATCACTCCT GTTCCTGGCG GCGTAGGGCC CATGACAGTT 
GCAATGCTCA TGCAGAGCAC AGTAGAGAGT GCCAAGCGTT TCCTGGAGAA ATTTAAGCCA 
GGAAAGTGGA TGATTCAGTA TAACAACCTT AACCTCAAGA CACCTGTTCC AAGTGACATT 
GATATATCAC GATCTTGTAA ACCGAAGCCC ATTGGTAAGC TGGCTCGAGA AATTGGTCTG 
CTGTCTGAAG AGGTAGAATT ATATGGTGAA ACAAAGGCCA AAGTTCTGCT GTCAGCACTA 
GAACGCCTGA AGCACCGGCC TGATGGGAAA TACGTGGTGG TGACTGGAAT AACTCCAACA 
CCCCTGGGAG AAGGGAAAAG CACAACTACA ATCGGGCTAG TGCAAGCCCT TGGTGCCCAT 
CTCTACCAGA ATGTCTTTGC GTGTGTGCGA CAGCCTTCTC AGGGCCCCAC CTTTGGAATA 
AAAGGTGGCG CTGCAGGAGG CGGCTACTCC CAGGTCATTC CTATGGAAGA GTTTAATCTC 
CACCTCACAG GTGACATCCA TGCCATCACT GCAGCTAATA ACCTCGTTGC TGCGGCCATT 
GATGCTCGGA TATTTCATGA ACTGACCCAG ACAGACAAGG CTCTCTTTAA TCGTTTGGTG 
CCATCAGTAA ATGGAGTGAG AAGGTTCTCT GACATCCAAA TCCGAAGGTT AAAGAGACTA 
GGCATTGAAA AGACTGACCC TACCACACTG ACAGATGAAG AGATAAACAG ATTTGCAAGA 
TTGGACATTG ATCCAGAAAC CATAACTTGG CAAAGAGTGT TGGATACCAA TGATAGATTC 
CTGAGGAAGA TCACGATTGG ACAGGCTCCA ACGGAGAAGG GTCACACACG GACGGCCCAG 
TTTGATATCT CTGTGGCCAG TGAAATTATG GCTGTCCTGG CTCTCACCAC TTCTCTAGAA 
GACATGAGAG AGAGACTGGG CAAAATGGTG GTGGCATCCA GTAAGAAAGG AGAGCCCGTC 
AGTGCCGAAG ATCTGGGGGT GAGTGGTGCA CTGACAGTGC TTATGAAGGA CGCAATCAAG 
CCCAATCTCA TGCAGACACT GGAGGGCACT CCAGTGTTTG TCCATGCTGG CCCGTTTGCC 
AACATCGCAC ATGGCAATTC CTCCATCATT GCAGACCGGA TCGCACTCAA GCTTGTTGGC 
CCAGAAGGGT TTGTAGTGAC GGAAGCAGGA TTTGGAGCAG ACATTGGAAT GGAAAAGTTT 
TTTAACATCA AATGCCGGTA TTCCGGCCTC TGCCCCCACG TGGTGGTGCT TGTTGCCACT 
GTCAGGGCTC TCAAGATGCA CGGGGGCGGC CCCACGGTCA CTGCTGGACT GCCTCTTCCC 
AAGGCTTACA TACAGGAGAA CCTGGAGCTG GTTGAAAAAG GCTTCAGTAA CTTGAAGAAA 
CAAATTGAAA ATGCCAGAAT GTTTGGAATT CCAGTAGTAG TGGCCGTGAA TGCATTCAAG 
ACGGATACAG AGTCTGAGCT GGACCTCATC AGCCGCCTTT CCAGAGAACA TGGGGCTTTT 
GATGCCGTGA AGTGCACTCA CTGGGCAGAA GGGGGCAAGG GTGCCTTAGC CCTGGCTCAG 
GCCGTCCAGA GAGCAGCACA AGCACCCAGC AGCTTCCAGC TCCTTTATGA CCTCAAGCTC 
CCAGTTGAGG ATAAAATCAG GATCATTGCA CAGAAGATCT ATGGAGCAGA TGACATTGAA 
TTACTTCCCG AAGCTCAACA CAAAGCTGAA GTCTACACGA AGCAGGGCTT TGGGAATCTC 
CCCATCTGCA TGGCTAAAAC ACACTTGTCT TTGTCTCACA ACCCAGAGCA AAAAGGTGTC 
CCTACAGGCT TCATTCTGCC CATTCGCGAC ATCCGCGCCA GCGTTGGGGC TGGTTTTCTG 
TACCCCTTAG TAGGAACGAT CACCATCCAT CTTCAAGAAG CTACTTTGAA AGTCTGGCCA 
GTGTCTATTC AGGCCCACTG GGAGTTAGGA AGTATAAGTA AGCCAAGAGA AGTCAGCCCC 
TGCCCAGAAG ATCTGAAACT AATAGTAGGA GTTTCCCCAG AAGTCATTTT CAGCCTTAAT 
TCTCATCATG TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MTHFD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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