MDH1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MDH1
Entrez Gene ID	4190
Full Name	malate dehydrogenase 1
Synonyms	HEL-S-32,MDH-s,MDHA,MGC:1375,MOR2
General protein information	Preferred Names malate dehydrogenase 1 Names malate dehydrogenase, cytoplasmic malate dehydrogenase, peroxisomal cytosolic malate dehydrogenase diiodophenylpyruvate reductase epididymis secretory protein Li 32 malate dehydrogenase 1, NAD (soluble) soluble malate dehydrogenase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p15
Summary	This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Feb 2016].
Disorder MIM:	154200

mRNA and Protein(s)

mRNA	Protein	Name
NM_001316374.2	NP_001303303.1	malate dehydrogenase, peroxisomal isoform MDH1x
NM_005917.3	NP_005908.1	malate dehydrogenase, cytoplasmic isoform MDH1
NM_005917.4	NP_005908.1	malate dehydrogenase, cytoplasmic isoform MDH1
NM_001199112.1	NP_001186041.1	malate dehydrogenase, cytoplasmic isoform 3
NM_001316374.1	NP_001303303.1	malate dehydrogenase, peroxisomal isoform MDH1x
NM_001199111.1	NP_001186040.1	malate dehydrogenase, cytoplasmic isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MDH1	XP_531844.1
Bos taurus (cattle)	MDH1	NP_001029800.1
Caenorhabditis elegans (roundworm)	mdh-1	NP_504656.1
Xenopus tropicalis (tropical clawed frog)	mdh1	NP_001006694.1
	MDH1	XP_001084973.1
Arabidopsis thaliana (thale cress)	AT5G43330	NP_199147.1
Pan troglodytes (chimpanzee)	MDH1	NP_001229997.1
Homo sapiens (human)	MDH1	NP_001186040.1
Gallus gallus (chicken)	MDH1	NP_001006395.1
Oryza sativa (rice)	Os10g0478200	NP_001064860.1
Mus musculus (house mouse)	Mdh1	NP_032644.3
Rattus norvegicus (Norway rat)	Mdh1	NP_150238.1
Danio rerio (zebrafish)	mdh1b	NP_956263.1
Drosophila melanogaster (fruit fly)	Mdh1	NP_609394.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009510	XP_310186.3
Arabidopsis thaliana (thale cress)	AT1G04410	NP_171936.1

Gene Ontology
Bibliography

Related articles in PubMed

Cytosolic malate dehydrogenase activity helps support glycolysis in actively proliferating cells and cancer.
Hanse EA, Ruan C, Kachman M, Wang D, Lowman XH, Kelekar A
Oncogene36(27)3915-3924(2017 Jul)

Arginine Methylation of MDH1 by CARM1 Inhibits Glutamine Metabolism and Suppresses Pancreatic Cancer.
Wang YP, Zhou W, Wang J, Huang X, Zuo Y, Wang TS, Gao X, Xu YY, Zou SW, Liu YB, Cheng JK, Lei QY
Molecular cell64(4)673-687(2016 Nov)

The functional readthrough extension of malate dehydrogenase reveals a modification of the genetic code.
Hofhuis J, Schueren F, N?tzel C, Lingner T, G?rtner J, Jahn O, Thoms S
Open biology6(11)(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Proliferating cells rely on both MDH1 and LDH to replenish cytosolic NAD.
Title: Cytosolic malate dehydrogenase activity helps support glycolysis in actively proliferating cells and cancer.

Arginine methylation of MDH1 by CARM1 regulates cellular redox homeostasis and suppresses glutamine metabolism of pancreatic cancer.
Title: Arginine Methylation of MDH1 by CARM1 Inhibits Glutamine Metabolism and Suppresses Pancreatic Cancer.

Data show that in the endogenous readthrough of the human MDH1 stop codon, the stop codon can encode tryptophan and arginine, and is tissue-specific.
Title: The functional readthrough extension of malate dehydrogenase reveals a modification of the genetic code.

adipogenic differentiation may be regulated by the acetylation of MDH1
Title: Acetylation of malate dehydrogenase 1 promotes adipogenic differentiation via activating its enzymatic activity.

A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration.
Title: Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration.

The following MDH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MDH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05940	NM_001199111.1 Latest version!	Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$144.50-$202.30 $289.00
OHu05941	NM_001199112.1 Latest version!	Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
OHu13813	NM_005917.4 Latest version!	Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu100964	NM_001316374.2 Latest version!	Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu13813	NM_005917.3	Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu100964	NM_001316374.1	Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MDH1 ( NM_001199111.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186040.1 Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu05940
Clone ID Related Accession (Same CDS sequence)	NM_001199111.1
Accession Version	NM_001199111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	malate dehydrogenase, cytoplasmic isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI667299.1, AK295931.1 and BC001484.2. Transcript Variant: This variant (2) contains a different 5' terminal exon, which results in translation initiation from an alternate start codon compared to variant 1. The encoded isoform (2) is longer with a distinct N-terminus compared to isoform MDH1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.383670.1, SRR1163657.241771.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGACGCT GCAGCTATTT TCCAAAGGAC GTTACGGTGT TTGATAAGGA CGATAAGTCT 
GAACCAATCA GAGTCCTTGT GACTGGAGCA GCTGGTCAAA TTGCATATTC ACTGCTGTAC 
AGTATTGGAA ATGGATCTGT CTTTGGTAAA GATCAGCCTA TAATTCTTGT GCTGTTGGAT 
ATCACCCCCA TGATGGGTGT CCTGGACGGT GTCCTAATGG AACTGCAAGA CTGTGCCCTT 
CCCCTCCTGA AAGATGTCAT CGCAACAGAT AAAGAAGACG TTGCCTTCAA AGACCTGGAT 
GTGGCCATTC TTGTGGGCTC CATGCCAAGA AGGGAAGGCA TGGAGAGAAA AGATTTACTG 
AAAGCAAATG TGAAAATCTT CAAATCCCAG GGTGCAGCCT TAGATAAATA CGCCAAGAAG 
TCAGTTAAGG TTATTGTTGT GGGTAATCCA GCCAATACCA ACTGCCTGAC TGCTTCCAAG 
TCAGCTCCAT CCATCCCCAA GGAGAACTTC AGTTGCTTGA CTCGTTTGGA TCACAACCGA 
GCTAAAGCTC AAATTGCTCT TAAACTTGGT GTGACTGCTA ATGATGTAAA GAATGTCATT 
ATCTGGGGAA ACCATTCCTC GACTCAGTAT CCAGATGTCA ACCATGCCAA GGTGAAATTG 
CAAGGAAAGG AAGTTGGTGT TTATGAAGCT CTGAAAGATG ACAGCTGGCT CAAGGGAGAA 
TTTGTCACGA CTGTGCAGCA GCGTGGCGCT GCTGTCATCA AGGCTCGAAA ACTATCCAGT 
GCCATGTCTG CTGCAAAAGC CATCTGTGAC CACGTCAGGG ACATCTGGTT TGGAACCCCA 
GAGGGAGAGT TTGTGTCCAT GGGTGTTATC TCTGATGGCA ACTCCTATGG TGTTCCTGAT 
GATCTGCTCT ACTCATTCCC TGTTGTAATC AAGAATAAGA CCTGGAAGTT TGTTGAAGGT 
CTCCCTATTA ATGATTTCTC ACGTGAGAAG ATGGATCTTA CTGCAAAGGA ACTGACAGAA 
GAAAAAGAAA GTGCTTTTGA ATTTCTTTCC TCTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186040.1
CDS	178..1236
Translation	MRRCSYFPKDVTVFDKDDKSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

Target ORF information:

RefSeq Version	NM_001199111.1
Organism	Homo sapiens(human)
Definition	Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199111.1

ORF Insert Sequence:

ATGCGACGCT GCAGCTATTT TCCAAAGGAC GTTACGGTGT TTGATAAGGA CGATAAGTCT 
GAACCAATCA GAGTCCTTGT GACTGGAGCA GCTGGTCAAA TTGCATATTC ACTGCTGTAC 
AGTATTGGAA ATGGATCTGT CTTTGGTAAA GATCAGCCTA TAATTCTTGT GCTGTTGGAT 
ATCACCCCCA TGATGGGTGT CCTGGACGGT GTCCTAATGG AACTGCAAGA CTGTGCCCTT 
CCCCTCCTGA AAGATGTCAT CGCAACAGAT AAAGAAGACG TTGCCTTCAA AGACCTGGAT 
GTGGCCATTC TTGTGGGCTC CATGCCAAGA AGGGAAGGCA TGGAGAGAAA AGATTTACTG 
AAAGCAAATG TGAAAATCTT CAAATCCCAG GGTGCAGCCT TAGATAAATA CGCCAAGAAG 
TCAGTTAAGG TTATTGTTGT GGGTAATCCA GCCAATACCA ACTGCCTGAC TGCTTCCAAG 
TCAGCTCCAT CCATCCCCAA GGAGAACTTC AGTTGCTTGA CTCGTTTGGA TCACAACCGA 
GCTAAAGCTC AAATTGCTCT TAAACTTGGT GTGACTGCTA ATGATGTAAA GAATGTCATT 
ATCTGGGGAA ACCATTCCTC GACTCAGTAT CCAGATGTCA ACCATGCCAA GGTGAAATTG 
CAAGGAAAGG AAGTTGGTGT TTATGAAGCT CTGAAAGATG ACAGCTGGCT CAAGGGAGAA 
TTTGTCACGA CTGTGCAGCA GCGTGGCGCT GCTGTCATCA AGGCTCGAAA ACTATCCAGT 
GCCATGTCTG CTGCAAAAGC CATCTGTGAC CACGTCAGGG ACATCTGGTT TGGAACCCCA 
GAGGGAGAGT TTGTGTCCAT GGGTGTTATC TCTGATGGCA ACTCCTATGG TGTTCCTGAT 
GATCTGCTCT ACTCATTCCC TGTTGTAATC AAGAATAAGA CCTGGAAGTT TGTTGAAGGT 
CTCCCTATTA ATGATTTCTC ACGTGAGAAG ATGGATCTTA CTGCAAAGGA ACTGACAGAA 
GAAAAAGAAA GTGCTTTTGA ATTTCTTTCC TCTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDH1 ( NM_001199112.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186041.1 Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu05941
Clone ID Related Accession (Same CDS sequence)	NM_001199112.1
Accession Version	NM_001199112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	malate dehydrogenase, cytoplasmic isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI667299.1, AK300719.1 and BC001484.2. Transcript Variant: This variant (3) lacks an exon in the 5' region, which results in translation initiation from an in-frame downstream start codon compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform MDH1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300719.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCAAGAA GGGAAGGCAT GGAGAGAAAA GATTTACTGA AAGCAAATGT GAAAATCTTC 
AAATCCCAGG GTGCAGCCTT AGATAAATAC GCCAAGAAGT CAGTTAAGGT TATTGTTGTG 
GGTAATCCAG CCAATACCAA CTGCCTGACT GCTTCCAAGT CAGCTCCATC CATCCCCAAG 
GAGAACTTCA GTTGCTTGAC TCGTTTGGAT CACAACCGAG CTAAAGCTCA AATTGCTCTT 
AAACTTGGTG TGACTGCTAA TGATGTAAAG AATGTCATTA TCTGGGGAAA CCATTCCTCG 
ACTCAGTATC CAGATGTCAA CCATGCCAAG GTGAAATTGC AAGGAAAGGA AGTTGGTGTT 
TATGAAGCTC TGAAAGATGA CAGCTGGCTC AAGGGAGAAT TTGTCACGAC TGTGCAGCAG 
CGTGGCGCTG CTGTCATCAA GGCTCGAAAA CTATCCAGTG CCATGTCTGC TGCAAAAGCC 
ATCTGTGACC ACGTCAGGGA CATCTGGTTT GGAACCCCAG AGGGAGAGTT TGTGTCCATG 
GGTGTTATCT CTGATGGCAA CTCCTATGGT GTTCCTGATG ATCTGCTCTA CTCATTCCCT 
GTTGTAATCA AGAATAAGAC CTGGAAGTTT GTTGAAGGTC TCCCTATTAA TGATTTCTCA 
CGTGAGAAGA TGGATCTTAC TGCAAAGGAA CTGACAGAAG AAAAAGAAAG TGCTTTTGAA 
TTTCTTTCCT CTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186041.1
CDS	402..1139
Translation	MPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

Target ORF information:

RefSeq Version	NM_001199112.1
Organism	Homo sapiens(human)
Definition	Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199112.1

ORF Insert Sequence:

ATGCCAAGAA GGGAAGGCAT GGAGAGAAAA GATTTACTGA AAGCAAATGT GAAAATCTTC 
AAATCCCAGG GTGCAGCCTT AGATAAATAC GCCAAGAAGT CAGTTAAGGT TATTGTTGTG 
GGTAATCCAG CCAATACCAA CTGCCTGACT GCTTCCAAGT CAGCTCCATC CATCCCCAAG 
GAGAACTTCA GTTGCTTGAC TCGTTTGGAT CACAACCGAG CTAAAGCTCA AATTGCTCTT 
AAACTTGGTG TGACTGCTAA TGATGTAAAG AATGTCATTA TCTGGGGAAA CCATTCCTCG 
ACTCAGTATC CAGATGTCAA CCATGCCAAG GTGAAATTGC AAGGAAAGGA AGTTGGTGTT 
TATGAAGCTC TGAAAGATGA CAGCTGGCTC AAGGGAGAAT TTGTCACGAC TGTGCAGCAG 
CGTGGCGCTG CTGTCATCAA GGCTCGAAAA CTATCCAGTG CCATGTCTGC TGCAAAAGCC 
ATCTGTGACC ACGTCAGGGA CATCTGGTTT GGAACCCCAG AGGGAGAGTT TGTGTCCATG 
GGTGTTATCT CTGATGGCAA CTCCTATGGT GTTCCTGATG ATCTGCTCTA CTCATTCCCT 
GTTGTAATCA AGAATAAGAC CTGGAAGTTT GTTGAAGGTC TCCCTATTAA TGATTTCTCA 
CGTGAGAAGA TGGATCTTAC TGCAAAGGAA CTGACAGAAG AAAAAGAAAG TGCTTTTGAA 
TTTCTTTCCT CTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDH1 ( NM_005917.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005908.1 Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13813
Clone ID Related Accession (Same CDS sequence)	NM_005917.3 , NM_005917.4
Accession Version	NM_005917.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-21
Organism	Homo sapiens(human)
Product	malate dehydrogenase, cytoplasmic isoform MDH1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB266998.1 and BC001484.2. On Nov 20, 2010 this sequence version replaced NM_005917.2. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MDH1) results from translation termination at the upstream UGA stop codon, while the longer isoform (MDH1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (MDH1), which is localized in the cytosol. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.273195.1, SRR1163655.377309.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGAAC CAATCAGAGT CCTTGTGACT GGAGCAGCTG GTCAAATTGC ATATTCACTG 
CTGTACAGTA TTGGAAATGG ATCTGTCTTT GGTAAAGATC AGCCTATAAT TCTTGTGCTG 
TTGGATATCA CCCCCATGAT GGGTGTCCTG GACGGTGTCC TAATGGAACT GCAAGACTGT 
GCCCTTCCCC TCCTGAAAGA TGTCATCGCA ACAGATAAAG AAGACGTTGC CTTCAAAGAC 
CTGGATGTGG CCATTCTTGT GGGCTCCATG CCAAGAAGGG AAGGCATGGA GAGAAAAGAT 
TTACTGAAAG CAAATGTGAA AATCTTCAAA TCCCAGGGTG CAGCCTTAGA TAAATACGCC 
AAGAAGTCAG TTAAGGTTAT TGTTGTGGGT AATCCAGCCA ATACCAACTG CCTGACTGCT 
TCCAAGTCAG CTCCATCCAT CCCCAAGGAG AACTTCAGTT GCTTGACTCG TTTGGATCAC 
AACCGAGCTA AAGCTCAAAT TGCTCTTAAA CTTGGTGTGA CTGCTAATGA TGTAAAGAAT 
GTCATTATCT GGGGAAACCA TTCCTCGACT CAGTATCCAG ATGTCAACCA TGCCAAGGTG 
AAATTGCAAG GAAAGGAAGT TGGTGTTTAT GAAGCTCTGA AAGATGACAG CTGGCTCAAG 
GGAGAATTTG TCACGACTGT GCAGCAGCGT GGCGCTGCTG TCATCAAGGC TCGAAAACTA 
TCCAGTGCCA TGTCTGCTGC AAAAGCCATC TGTGACCACG TCAGGGACAT CTGGTTTGGA 
ACCCCAGAGG GAGAGTTTGT GTCCATGGGT GTTATCTCTG ATGGCAACTC CTATGGTGTT 
CCTGATGATC TGCTCTACTC ATTCCCTGTT GTAATCAAGA ATAAGACCTG GAAGTTTGTT 
GAAGGTCTCC CTATTAATGA TTTCTCACGT GAGAAGATGG ATCTTACTGC AAAGGAACTG 
ACAGAAGAAA AAGAAAGTGC TTTTGAATTT CTTTCCTCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005908.1
CDS	436..1440
Translation	MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

Target ORF information:

RefSeq Version	NM_005917.3
Organism	Homo sapiens(human)
Definition	Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005917.3

ORF Insert Sequence:

ATGTCTGAAC CAATCAGAGT CCTTGTGACT GGAGCAGCTG GTCAAATTGC ATATTCACTG 
CTGTACAGTA TTGGAAATGG ATCTGTCTTT GGTAAAGATC AGCCTATAAT TCTTGTGCTG 
TTGGATATCA CCCCCATGAT GGGTGTCCTG GACGGTGTCC TAATGGAACT GCAAGACTGT 
GCCCTTCCCC TCCTGAAAGA TGTCATCGCA ACAGATAAAG AAGACGTTGC CTTCAAAGAC 
CTGGATGTGG CCATTCTTGT GGGCTCCATG CCAAGAAGGG AAGGCATGGA GAGAAAAGAT 
TTACTGAAAG CAAATGTGAA AATCTTCAAA TCCCAGGGTG CAGCCTTAGA TAAATACGCC 
AAGAAGTCAG TTAAGGTTAT TGTTGTGGGT AATCCAGCCA ATACCAACTG CCTGACTGCT 
TCCAAGTCAG CTCCATCCAT CCCCAAGGAG AACTTCAGTT GCTTGACTCG TTTGGATCAC 
AACCGAGCTA AAGCTCAAAT TGCTCTTAAA CTTGGTGTGA CTGCTAATGA TGTAAAGAAT 
GTCATTATCT GGGGAAACCA TTCCTCGACT CAGTATCCAG ATGTCAACCA TGCCAAGGTG 
AAATTGCAAG GAAAGGAAGT TGGTGTTTAT GAAGCTCTGA AAGATGACAG CTGGCTCAAG 
GGAGAATTTG TCACGACTGT GCAGCAGCGT GGCGCTGCTG TCATCAAGGC TCGAAAACTA 
TCCAGTGCCA TGTCTGCTGC AAAAGCCATC TGTGACCACG TCAGGGACAT CTGGTTTGGA 
ACCCCAGAGG GAGAGTTTGT GTCCATGGGT GTTATCTCTG ATGGCAACTC CTATGGTGTT 
CCTGATGATC TGCTCTACTC ATTCCCTGTT GTAATCAAGA ATAAGACCTG GAAGTTTGTT 
GAAGGTCTCC CTATTAATGA TTTCTCACGT GAGAAGATGG ATCTTACTGC AAAGGAACTG 
ACAGAAGAAA AAGAAAGTGC TTTTGAATTT CTTTCCTCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDH1 ( NM_005917.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005908.1 Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13813
Clone ID Related Accession (Same CDS sequence)	NM_005917.3 , NM_005917.4
Accession Version	NM_005917.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	malate dehydrogenase, cytoplasmic isoform MDH1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB266998.1 and BC001484.2. On Nov 22, 2018 this sequence version replaced NM_005917.3. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MDH1) results from translation termination at the upstream UGA stop codon, while the longer isoform (MDH1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (MDH1), which is localized in the cytosol. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1025765.1, SRR3476690.1059713.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000233114.13/ ENSP00000233114.8 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGAAC CAATCAGAGT CCTTGTGACT GGAGCAGCTG GTCAAATTGC ATATTCACTG 
CTGTACAGTA TTGGAAATGG ATCTGTCTTT GGTAAAGATC AGCCTATAAT TCTTGTGCTG 
TTGGATATCA CCCCCATGAT GGGTGTCCTG GACGGTGTCC TAATGGAACT GCAAGACTGT 
GCCCTTCCCC TCCTGAAAGA TGTCATCGCA ACAGATAAAG AAGACGTTGC CTTCAAAGAC 
CTGGATGTGG CCATTCTTGT GGGCTCCATG CCAAGAAGGG AAGGCATGGA GAGAAAAGAT 
TTACTGAAAG CAAATGTGAA AATCTTCAAA TCCCAGGGTG CAGCCTTAGA TAAATACGCC 
AAGAAGTCAG TTAAGGTTAT TGTTGTGGGT AATCCAGCCA ATACCAACTG CCTGACTGCT 
TCCAAGTCAG CTCCATCCAT CCCCAAGGAG AACTTCAGTT GCTTGACTCG TTTGGATCAC 
AACCGAGCTA AAGCTCAAAT TGCTCTTAAA CTTGGTGTGA CTGCTAATGA TGTAAAGAAT 
GTCATTATCT GGGGAAACCA TTCCTCGACT CAGTATCCAG ATGTCAACCA TGCCAAGGTG 
AAATTGCAAG GAAAGGAAGT TGGTGTTTAT GAAGCTCTGA AAGATGACAG CTGGCTCAAG 
GGAGAATTTG TCACGACTGT GCAGCAGCGT GGCGCTGCTG TCATCAAGGC TCGAAAACTA 
TCCAGTGCCA TGTCTGCTGC AAAAGCCATC TGTGACCACG TCAGGGACAT CTGGTTTGGA 
ACCCCAGAGG GAGAGTTTGT GTCCATGGGT GTTATCTCTG ATGGCAACTC CTATGGTGTT 
CCTGATGATC TGCTCTACTC ATTCCCTGTT GTAATCAAGA ATAAGACCTG GAAGTTTGTT 
GAAGGTCTCC CTATTAATGA TTTCTCACGT GAGAAGATGG ATCTTACTGC AAAGGAACTG 
ACAGAAGAAA AAGAAAGTGC TTTTGAATTT CTTTCCTCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005908.1
CDS	82..1086
Translation	MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

Target ORF information:

RefSeq Version	NM_005917.4
Organism	Homo sapiens(human)
Definition	Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005917.4

ORF Insert Sequence:

ATGTCTGAAC CAATCAGAGT CCTTGTGACT GGAGCAGCTG GTCAAATTGC ATATTCACTG 
CTGTACAGTA TTGGAAATGG ATCTGTCTTT GGTAAAGATC AGCCTATAAT TCTTGTGCTG 
TTGGATATCA CCCCCATGAT GGGTGTCCTG GACGGTGTCC TAATGGAACT GCAAGACTGT 
GCCCTTCCCC TCCTGAAAGA TGTCATCGCA ACAGATAAAG AAGACGTTGC CTTCAAAGAC 
CTGGATGTGG CCATTCTTGT GGGCTCCATG CCAAGAAGGG AAGGCATGGA GAGAAAAGAT 
TTACTGAAAG CAAATGTGAA AATCTTCAAA TCCCAGGGTG CAGCCTTAGA TAAATACGCC 
AAGAAGTCAG TTAAGGTTAT TGTTGTGGGT AATCCAGCCA ATACCAACTG CCTGACTGCT 
TCCAAGTCAG CTCCATCCAT CCCCAAGGAG AACTTCAGTT GCTTGACTCG TTTGGATCAC 
AACCGAGCTA AAGCTCAAAT TGCTCTTAAA CTTGGTGTGA CTGCTAATGA TGTAAAGAAT 
GTCATTATCT GGGGAAACCA TTCCTCGACT CAGTATCCAG ATGTCAACCA TGCCAAGGTG 
AAATTGCAAG GAAAGGAAGT TGGTGTTTAT GAAGCTCTGA AAGATGACAG CTGGCTCAAG 
GGAGAATTTG TCACGACTGT GCAGCAGCGT GGCGCTGCTG TCATCAAGGC TCGAAAACTA 
TCCAGTGCCA TGTCTGCTGC AAAAGCCATC TGTGACCACG TCAGGGACAT CTGGTTTGGA 
ACCCCAGAGG GAGAGTTTGT GTCCATGGGT GTTATCTCTG ATGGCAACTC CTATGGTGTT 
CCTGATGATC TGCTCTACTC ATTCCCTGTT GTAATCAAGA ATAAGACCTG GAAGTTTGTT 
GAAGGTCTCC CTATTAATGA TTTCTCACGT GAGAAGATGG ATCTTACTGC AAAGGAACTG 
ACAGAAGAAA AAGAAAGTGC TTTTGAATTT CTTTCCTCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDH1 ( NM_001316374.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303303.1 Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.

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CloneID	OHu100964
Clone ID Related Accession (Same CDS sequence)	NM_001316374.2 , NM_001316374.1
Accession Version	NM_001316374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	malate dehydrogenase, peroxisomal isoform MDH1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB266998.1 and BC001484.2. On May 31, 2019 this sequence version replaced NM_001316374.1. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MDH1) results from translation termination at the upstream UGA stop codon, while the longer isoform (MDH1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the longer, C-terminally extended isoform (MDH1x), which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.54585.1, SRR5189661.64373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: PMID: 25340584 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTGAAC CAATCAGAGT CCTTGTGACT GGAGCAGCTG GTCAAATTGC ATATTCACTG 
CTGTACAGTA TTGGAAATGG ATCTGTCTTT GGTAAAGATC AGCCTATAAT TCTTGTGCTG 
TTGGATATCA CCCCCATGAT GGGTGTCCTG GACGGTGTCC TAATGGAACT GCAAGACTGT 
GCCCTTCCCC TCCTGAAAGA TGTCATCGCA ACAGATAAAG AAGACGTTGC CTTCAAAGAC 
CTGGATGTGG CCATTCTTGT GGGCTCCATG CCAAGAAGGG AAGGCATGGA GAGAAAAGAT 
TTACTGAAAG CAAATGTGAA AATCTTCAAA TCCCAGGGTG CAGCCTTAGA TAAATACGCC 
AAGAAGTCAG TTAAGGTTAT TGTTGTGGGT AATCCAGCCA ATACCAACTG CCTGACTGCT 
TCCAAGTCAG CTCCATCCAT CCCCAAGGAG AACTTCAGTT GCTTGACTCG TTTGGATCAC 
AACCGAGCTA AAGCTCAAAT TGCTCTTAAA CTTGGTGTGA CTGCTAATGA TGTAAAGAAT 
GTCATTATCT GGGGAAACCA TTCCTCGACT CAGTATCCAG ATGTCAACCA TGCCAAGGTG 
AAATTGCAAG GAAAGGAAGT TGGTGTTTAT GAAGCTCTGA AAGATGACAG CTGGCTCAAG 
GGAGAATTTG TCACGACTGT GCAGCAGCGT GGCGCTGCTG TCATCAAGGC TCGAAAACTA 
TCCAGTGCCA TGTCTGCTGC AAAAGCCATC TGTGACCACG TCAGGGACAT CTGGTTTGGA 
ACCCCAGAGG GAGAGTTTGT GTCCATGGGT GTTATCTCTG ATGGCAACTC CTATGGTGTT 
CCTGATGATC TGCTCTACTC ATTCCCTGTT GTAATCAAGA ATAAGACCTG GAAGTTTGTT 
GAAGGTCTCC CTATTAATGA TTTCTCACGT GAGAAGATGG ATCTTACTGC AAAGGAACTG 
ACAGAAGAAA AAGAAAGTGC TTTTGAATTT CTTTCCTCTG CCTGACTAGA CAATGATGTT 
ACTAAATGCT TCAAAGCTGA AGAATCTAAA TGTCGTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303303.1
CDS	82..1143
Translation	MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA*LDNDVTKCFKAEESKCRL

Target ORF information:

RefSeq Version	NM_001316374.2
Organism	Homo sapiens(human)
Definition	Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316374.2

ORF Insert Sequence:

ATGTCTGAAC CAATCAGAGT CCTTGTGACT GGAGCAGCTG GTCAAATTGC ATATTCACTG 
CTGTACAGTA TTGGAAATGG ATCTGTCTTT GGTAAAGATC AGCCTATAAT TCTTGTGCTG 
TTGGATATCA CCCCCATGAT GGGTGTCCTG GACGGTGTCC TAATGGAACT GCAAGACTGT 
GCCCTTCCCC TCCTGAAAGA TGTCATCGCA ACAGATAAAG AAGACGTTGC CTTCAAAGAC 
CTGGATGTGG CCATTCTTGT GGGCTCCATG CCAAGAAGGG AAGGCATGGA GAGAAAAGAT 
TTACTGAAAG CAAATGTGAA AATCTTCAAA TCCCAGGGTG CAGCCTTAGA TAAATACGCC 
AAGAAGTCAG TTAAGGTTAT TGTTGTGGGT AATCCAGCCA ATACCAACTG CCTGACTGCT 
TCCAAGTCAG CTCCATCCAT CCCCAAGGAG AACTTCAGTT GCTTGACTCG TTTGGATCAC 
AACCGAGCTA AAGCTCAAAT TGCTCTTAAA CTTGGTGTGA CTGCTAATGA TGTAAAGAAT 
GTCATTATCT GGGGAAACCA TTCCTCGACT CAGTATCCAG ATGTCAACCA TGCCAAGGTG 
AAATTGCAAG GAAAGGAAGT TGGTGTTTAT GAAGCTCTGA AAGATGACAG CTGGCTCAAG 
GGAGAATTTG TCACGACTGT GCAGCAGCGT GGCGCTGCTG TCATCAAGGC TCGAAAACTA 
TCCAGTGCCA TGTCTGCTGC AAAAGCCATC TGTGACCACG TCAGGGACAT CTGGTTTGGA 
ACCCCAGAGG GAGAGTTTGT GTCCATGGGT GTTATCTCTG ATGGCAACTC CTATGGTGTT 
CCTGATGATC TGCTCTACTC ATTCCCTGTT GTAATCAAGA ATAAGACCTG GAAGTTTGTT 
GAAGGTCTCC CTATTAATGA TTTCTCACGT GAGAAGATGG ATCTTACTGC AAAGGAACTG 
ACAGAAGAAA AAGAAAGTGC TTTTGAATTT CTTTCCTCTG CCTGACTAGA CAATGATGTT 
ACTAAATGCT TCAAAGCTGA AGAATCTAAA TGTCGTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDH1 ( NM_001316374.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001303303.1 Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu100964
Clone ID Related Accession (Same CDS sequence)	NM_001316374.2 , NM_001316374.1
Accession Version	NM_001316374.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	malate dehydrogenase, peroxisomal isoform MDH1x
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB266998.1 and BC001484.2. Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MDH1) results from translation termination at the upstream UGA stop codon, while the longer isoform (MDH1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the longer, C-terminally extended isoform (MDH1x), which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.273195.1, SRR1163655.377309.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: PMID: 25340584 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTGAAC CAATCAGAGT CCTTGTGACT GGAGCAGCTG GTCAAATTGC ATATTCACTG 
CTGTACAGTA TTGGAAATGG ATCTGTCTTT GGTAAAGATC AGCCTATAAT TCTTGTGCTG 
TTGGATATCA CCCCCATGAT GGGTGTCCTG GACGGTGTCC TAATGGAACT GCAAGACTGT 
GCCCTTCCCC TCCTGAAAGA TGTCATCGCA ACAGATAAAG AAGACGTTGC CTTCAAAGAC 
CTGGATGTGG CCATTCTTGT GGGCTCCATG CCAAGAAGGG AAGGCATGGA GAGAAAAGAT 
TTACTGAAAG CAAATGTGAA AATCTTCAAA TCCCAGGGTG CAGCCTTAGA TAAATACGCC 
AAGAAGTCAG TTAAGGTTAT TGTTGTGGGT AATCCAGCCA ATACCAACTG CCTGACTGCT 
TCCAAGTCAG CTCCATCCAT CCCCAAGGAG AACTTCAGTT GCTTGACTCG TTTGGATCAC 
AACCGAGCTA AAGCTCAAAT TGCTCTTAAA CTTGGTGTGA CTGCTAATGA TGTAAAGAAT 
GTCATTATCT GGGGAAACCA TTCCTCGACT CAGTATCCAG ATGTCAACCA TGCCAAGGTG 
AAATTGCAAG GAAAGGAAGT TGGTGTTTAT GAAGCTCTGA AAGATGACAG CTGGCTCAAG 
GGAGAATTTG TCACGACTGT GCAGCAGCGT GGCGCTGCTG TCATCAAGGC TCGAAAACTA 
TCCAGTGCCA TGTCTGCTGC AAAAGCCATC TGTGACCACG TCAGGGACAT CTGGTTTGGA 
ACCCCAGAGG GAGAGTTTGT GTCCATGGGT GTTATCTCTG ATGGCAACTC CTATGGTGTT 
CCTGATGATC TGCTCTACTC ATTCCCTGTT GTAATCAAGA ATAAGACCTG GAAGTTTGTT 
GAAGGTCTCC CTATTAATGA TTTCTCACGT GAGAAGATGG ATCTTACTGC AAAGGAACTG 
ACAGAAGAAA AAGAAAGTGC TTTTGAATTT CTTTCCTCTG CCTGACTAGA CAATGATGTT 
ACTAAATGCT TCAAAGCTGA AGAATCTAAA TGTCGTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303303.1
CDS	436..1497
Translation	MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA*LDNDVTKCFKAEESKCRL

Target ORF information:

RefSeq Version	NM_001316374.1
Organism	Homo sapiens(human)
Definition	Homo sapiens malate dehydrogenase 1 (MDH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316374.1

ORF Insert Sequence:

ATGTCTGAAC CAATCAGAGT CCTTGTGACT GGAGCAGCTG GTCAAATTGC ATATTCACTG 
CTGTACAGTA TTGGAAATGG ATCTGTCTTT GGTAAAGATC AGCCTATAAT TCTTGTGCTG 
TTGGATATCA CCCCCATGAT GGGTGTCCTG GACGGTGTCC TAATGGAACT GCAAGACTGT 
GCCCTTCCCC TCCTGAAAGA TGTCATCGCA ACAGATAAAG AAGACGTTGC CTTCAAAGAC 
CTGGATGTGG CCATTCTTGT GGGCTCCATG CCAAGAAGGG AAGGCATGGA GAGAAAAGAT 
TTACTGAAAG CAAATGTGAA AATCTTCAAA TCCCAGGGTG CAGCCTTAGA TAAATACGCC 
AAGAAGTCAG TTAAGGTTAT TGTTGTGGGT AATCCAGCCA ATACCAACTG CCTGACTGCT 
TCCAAGTCAG CTCCATCCAT CCCCAAGGAG AACTTCAGTT GCTTGACTCG TTTGGATCAC 
AACCGAGCTA AAGCTCAAAT TGCTCTTAAA CTTGGTGTGA CTGCTAATGA TGTAAAGAAT 
GTCATTATCT GGGGAAACCA TTCCTCGACT CAGTATCCAG ATGTCAACCA TGCCAAGGTG 
AAATTGCAAG GAAAGGAAGT TGGTGTTTAT GAAGCTCTGA AAGATGACAG CTGGCTCAAG 
GGAGAATTTG TCACGACTGT GCAGCAGCGT GGCGCTGCTG TCATCAAGGC TCGAAAACTA 
TCCAGTGCCA TGTCTGCTGC AAAAGCCATC TGTGACCACG TCAGGGACAT CTGGTTTGGA 
ACCCCAGAGG GAGAGTTTGT GTCCATGGGT GTTATCTCTG ATGGCAACTC CTATGGTGTT 
CCTGATGATC TGCTCTACTC ATTCCCTGTT GTAATCAAGA ATAAGACCTG GAAGTTTGTT 
GAAGGTCTCC CTATTAATGA TTTCTCACGT GAGAAGATGG ATCTTACTGC AAAGGAACTG 
ACAGAAGAAA AAGAAAGTGC TTTTGAATTT CTTTCCTCTG CCTGACTAGA CAATGATGTT 
ACTAAATGCT TCAAAGCTGA AGAATCTAAA TGTCGTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cytosolic malate dehydrogenase activity helps support glycolysis in actively proliferating cells and cancer.
Oncogene36(27)3915-3924(2017 Jul)
Hanse EA,Ruan C,Kachman M,Wang D,Lowman XH,Kelekar A

Arginine Methylation of MDH1 by CARM1 Inhibits Glutamine Metabolism and Suppresses Pancreatic Cancer.
Molecular cell64(4)673-687(2016 Nov)
Wang YP,Zhou W,Wang J,Huang X,Zuo Y,Wang TS,Gao X,Xu YY,Zou SW,Liu YB,Cheng JK,Lei QY

The functional readthrough extension of malate dehydrogenase reveals a modification of the genetic code.
Open biology6(11)(2016 Nov)
Hofhuis J,Schueren F,N?tzel C,Lingner T,G?rtner J,Jahn O,Thoms S

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MDH1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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