MAN2A1 cDNA ORF clone, Homo sapiens(human)

The following MAN2A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN2A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu58846 XM_011543395.3
Latest version!
Homo sapiens mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu105666 XM_024446048.1
Latest version!
Homo sapiens mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu10839 NM_002372.3
Latest version!
Homo sapiens mannosidase alpha class 2A member 1 (MAN2A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu58845 XM_017009472.1
Latest version!
Homo sapiens mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu58846 XM_011543395.2 Homo sapiens mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu58846
    Clone ID Related Accession (Same CDS sequence) XM_011543395.2 , XM_011543395.3
    Accession Version XM_011543395.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2655bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543395.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAAGTTAA GCCGCCAGTT CACCGTGTTC GGCAGTGCGA TCTTCTGTGT GGTGATTTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTAGACTACC CCAGGAACCC GCGCCGCGAG
    GGCTCCTTCC CTCAGGGCCA GCTCTCAATG TTGCAAGAAA AAATAGACCA TTTGGAGCGT
    TTGCTAGCTG AGAATAATGA GATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT
    GAGTCTGTGG AGGATGGTCC GAAAAGTTCA CAAAGCAATT TCAGCCAAGG TGCTGGCTCA
    CATCTTCTGC CCTCACAATT ATCCCTCTCA GTTGACACTG CAGACTGTCT GTTTGCTTCA
    CAAAGTGGAA GTCACAATTC AGATGTGCAG ATGTTGGATG TTTACAGTCT AATTTCTTTT
    GACAATCCAG ATGGTGGAGT TTGGAAGCAA GGATTTGACA TTACTTATGA ATCTAATGAA
    TGGGACACTG AACCCCTTCA AGTCTTTGTG GTGCCTCATT CCCATAACGA CCCAGGTTGG
    TTGAAGACTT TCAATGACTA CTTTAGAGAC AAGACTCAGT ATATTTTTAA TAACATGGTC
    CTAAAGCTGA AAGAAGACTC ACGGAGGAAG TTTATTTGGT CTGAGATCTC TTACCTTTCA
    AAGTGGTGGG ATATTATAGA TATTCAGAAG AAGGATGCTG TTAAAAGTTT AATAGAAAAT
    GGTCAGCTTG AAATTGTGAC AGGTGGCTGG GTTATGCCTG ATGAAGCTAC TCCACATTAT
    TTTGCCTTAA TTGATCAACT AATTGAAGGA CATCAGTGGC TGGAAAATAA TATAGGAGTG
    AAACCTCGGT CCGGCTGGGC TATTGATCCC TTTGGACACT CACCAACAAT GGCTTATCTT
    CTAAACCGTG CTGGACTTTC TCACATGCTT ATCCAGAGAG TTCATTATGC AGTTAAAAAA
    CACTTTGCAC TGCATAAAAC ATTGGAGTTT TTTTGGAGAC AGAATTGGGA TCTGGGATCT
    GTCACAGATA TTTTATGCCA CATGATGCCC TTCTACAGCT ATGACATCCC TCACACTTGT
    GGACCTGATC CTAAAATATG CTGCCAGTTT GATTTTAAAC GTCTTCCTGG AGGCAGATTT
    GGTTGTCCCT GGGGAGTCCC CCCAGAAACA ATACATCCTG GAAATGTCCA AAGCAGGGCT
    CGGATGCTAC TAGATCAGTA CCGAAAGAAG TCAAAGCTTT TTCGTACCAA AGTTCTCCTG
    GCTCCACTAG GAGATGATTT CCGCTACTGT GAATACACGG AATGGGATTT ACAGTTTAAG
    AATTATCAGC AGCTTTTTGA TTATATGAAT TCTCAGTCCA AGTTTAAAGT TAAGATACAG
    TTTGGAACTT TATCAGATTT TTTTGATGCG CTGGATAAAG CAGATGAAAC TCAGAGAGAC
    AAGGGCCAAT CGATGTTCCC TGTTTTAAGT GGAGATTTTT TCACTTATGC CGATCGAGAT
    GATCATTACT GGAGTGGCTA TTTTACATCC AGACCCTTTT ACAAACGAAT GGACAGAATC
    ATGGAATCTC ATTTAAGGGC TGCTGAAATT CTTTACTATT TCGCCCTGAG ACAAGCTCAC
    AAATACAAGA TAAATAAATT TCTCTCATCA TCACTTTACA CGGCACTGAC AGAAGCCAGA
    AGGAATTTGG GACTGTTTCA ACATCATGAT GCTATCACAG GAACTGCAAA AGACTGGGTG
    GTTGTGGATT ATGGTACCAG ACTTTTTCAT TCGTTAATGG TTTTGGAGAA GATAATTGGA
    AATTCTGCAT TTCTTCTTAT TTTGAAGGAC AAACTCACAT ACGACTCTTA CTCTCCTGAT
    ACCTTCCTGG AGATGGATTT GAAACAAAAA TCACAAGATT CTCTGCCACA AAAAAATATA
    ATAAGGCTGA GTGCGGAGCC AAGGTACCTT GTGGTCTATA ATCCTTTAGA ACAAGACCGA
    ATCTCGTTGG TCTCAGTCTA TGTGAGTTCC CCGACAGTGC AAGTGTTCTC TGCTTCAGGA
    AAACCTGTGG AAGTTCAAGT CAGCGCAGTT TGGGATACAG CAAATACTAT TTCAGAAACA
    GCCTATGAGA TCTCTTTTCG AGCACATATA CCGCCATTGG GACTGAAAGT GTATAAGATT
    TTGGAATCAG CAAGTTCAAA TTCACATTTA GCTGATTATG TCTTGTATAA GAATAAAGTA
    GAAGATAGCG GAATTTTCAC CATAAAGAAT ATGATAAATA CTGAAGAAGG TATAACACTA
    GAGAACTCCT TTGTTTTACT TCGGTTTGAT CAAACTGGAC TTATGAAGCA AATGATGACT
    AAAGAAGATG GTAAACACCA TGAAGTAAAT GTGCAATTTT CATGGTATGG AACCACAATT
    AAAAGAGACA AAAGTGGTGC CTACCTCTTC TTACCTGATG GTAATGCCAA GCCTTATGTT
    TACACAACAC CGCCCTTTGT CAGAGTGACA CATGGAAGGA TTTATTCGGA AGTGACTTGC
    TTTTTTGACC ATGTTACTCA TAGAGTCCGA CTATACCACA TACAGGGCAT GAAAGAGAAG
    GTTATTTTAA GGGACTGCAA GCACATTCTG GTTACACAGA TTCAGAACTC AGAGGTTATC
    TTTGGGGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011541697.1
    CDS492..3146
    Translation

    Target ORF information:

    RefSeq Version XM_011543395.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011543395.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAAGTTAA GCCGCCAGTT CACCGTGTTC GGCAGTGCGA TCTTCTGTGT GGTGATTTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTAGACTACC CCAGGAACCC GCGCCGCGAG
    GGCTCCTTCC CTCAGGGCCA GCTCTCAATG TTGCAAGAAA AAATAGACCA TTTGGAGCGT
    TTGCTAGCTG AGAATAATGA GATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT
    GAGTCTGTGG AGGATGGTCC GAAAAGTTCA CAAAGCAATT TCAGCCAAGG TGCTGGCTCA
    CATCTTCTGC CCTCACAATT ATCCCTCTCA GTTGACACTG CAGACTGTCT GTTTGCTTCA
    CAAAGTGGAA GTCACAATTC AGATGTGCAG ATGTTGGATG TTTACAGTCT AATTTCTTTT
    GACAATCCAG ATGGTGGAGT TTGGAAGCAA GGATTTGACA TTACTTATGA ATCTAATGAA
    TGGGACACTG AACCCCTTCA AGTCTTTGTG GTGCCTCATT CCCATAACGA CCCAGGTTGG
    TTGAAGACTT TCAATGACTA CTTTAGAGAC AAGACTCAGT ATATTTTTAA TAACATGGTC
    CTAAAGCTGA AAGAAGACTC ACGGAGGAAG TTTATTTGGT CTGAGATCTC TTACCTTTCA
    AAGTGGTGGG ATATTATAGA TATTCAGAAG AAGGATGCTG TTAAAAGTTT AATAGAAAAT
    GGTCAGCTTG AAATTGTGAC AGGTGGCTGG GTTATGCCTG ATGAAGCTAC TCCACATTAT
    TTTGCCTTAA TTGATCAACT AATTGAAGGA CATCAGTGGC TGGAAAATAA TATAGGAGTG
    AAACCTCGGT CCGGCTGGGC TATTGATCCC TTTGGACACT CACCAACAAT GGCTTATCTT
    CTAAACCGTG CTGGACTTTC TCACATGCTT ATCCAGAGAG TTCATTATGC AGTTAAAAAA
    CACTTTGCAC TGCATAAAAC ATTGGAGTTT TTTTGGAGAC AGAATTGGGA TCTGGGATCT
    GTCACAGATA TTTTATGCCA CATGATGCCC TTCTACAGCT ATGACATCCC TCACACTTGT
    GGACCTGATC CTAAAATATG CTGCCAGTTT GATTTTAAAC GTCTTCCTGG AGGCAGATTT
    GGTTGTCCCT GGGGAGTCCC CCCAGAAACA ATACATCCTG GAAATGTCCA AAGCAGGGCT
    CGGATGCTAC TAGATCAGTA CCGAAAGAAG TCAAAGCTTT TTCGTACCAA AGTTCTCCTG
    GCTCCACTAG GAGATGATTT CCGCTACTGT GAATACACGG AATGGGATTT ACAGTTTAAG
    AATTATCAGC AGCTTTTTGA TTATATGAAT TCTCAGTCCA AGTTTAAAGT TAAGATACAG
    TTTGGAACTT TATCAGATTT TTTTGATGCG CTGGATAAAG CAGATGAAAC TCAGAGAGAC
    AAGGGCCAAT CGATGTTCCC TGTTTTAAGT GGAGATTTTT TCACTTATGC CGATCGAGAT
    GATCATTACT GGAGTGGCTA TTTTACATCC AGACCCTTTT ACAAACGAAT GGACAGAATC
    ATGGAATCTC ATTTAAGGGC TGCTGAAATT CTTTACTATT TCGCCCTGAG ACAAGCTCAC
    AAATACAAGA TAAATAAATT TCTCTCATCA TCACTTTACA CGGCACTGAC AGAAGCCAGA
    AGGAATTTGG GACTGTTTCA ACATCATGAT GCTATCACAG GAACTGCAAA AGACTGGGTG
    GTTGTGGATT ATGGTACCAG ACTTTTTCAT TCGTTAATGG TTTTGGAGAA GATAATTGGA
    AATTCTGCAT TTCTTCTTAT TTTGAAGGAC AAACTCACAT ACGACTCTTA CTCTCCTGAT
    ACCTTCCTGG AGATGGATTT GAAACAAAAA TCACAAGATT CTCTGCCACA AAAAAATATA
    ATAAGGCTGA GTGCGGAGCC AAGGTACCTT GTGGTCTATA ATCCTTTAGA ACAAGACCGA
    ATCTCGTTGG TCTCAGTCTA TGTGAGTTCC CCGACAGTGC AAGTGTTCTC TGCTTCAGGA
    AAACCTGTGG AAGTTCAAGT CAGCGCAGTT TGGGATACAG CAAATACTAT TTCAGAAACA
    GCCTATGAGA TCTCTTTTCG AGCACATATA CCGCCATTGG GACTGAAAGT GTATAAGATT
    TTGGAATCAG CAAGTTCAAA TTCACATTTA GCTGATTATG TCTTGTATAA GAATAAAGTA
    GAAGATAGCG GAATTTTCAC CATAAAGAAT ATGATAAATA CTGAAGAAGG TATAACACTA
    GAGAACTCCT TTGTTTTACT TCGGTTTGAT CAAACTGGAC TTATGAAGCA AATGATGACT
    AAAGAAGATG GTAAACACCA TGAAGTAAAT GTGCAATTTT CATGGTATGG AACCACAATT
    AAAAGAGACA AAAGTGGTGC CTACCTCTTC TTACCTGATG GTAATGCCAA GCCTTATGTT
    TACACAACAC CGCCCTTTGT CAGAGTGACA CATGGAAGGA TTTATTCGGA AGTGACTTGC
    TTTTTTGACC ATGTTACTCA TAGAGTCCGA CTATACCACA TACAGGGCAT GAAAGAGAAG
    GTTATTTTAA GGGACTGCAA GCACATTCTG GTTACACAGA TTCAGAACTC AGAGGTTATC
    TTTGGGGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58846
    Clone ID Related Accession (Same CDS sequence) XM_011543395.2 , XM_011543395.3
    Accession Version XM_011543395.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2655bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011543395.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAAGTTAA GCCGCCAGTT CACCGTGTTC GGCAGTGCGA TCTTCTGTGT GGTGATTTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTAGACTACC CCAGGAACCC GCGCCGCGAG
    GGCTCCTTCC CTCAGGGCCA GCTCTCAATG TTGCAAGAAA AAATAGACCA TTTGGAGCGT
    TTGCTAGCTG AGAATAATGA GATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT
    GAGTCTGTGG AGGATGGTCC GAAAAGTTCA CAAAGCAATT TCAGCCAAGG TGCTGGCTCA
    CATCTTCTGC CCTCACAATT ATCCCTCTCA GTTGACACTG CAGACTGTCT GTTTGCTTCA
    CAAAGTGGAA GTCACAATTC AGATGTGCAG ATGTTGGATG TTTACAGTCT AATTTCTTTT
    GACAATCCAG ATGGTGGAGT TTGGAAGCAA GGATTTGACA TTACTTATGA ATCTAATGAA
    TGGGACACTG AACCCCTTCA AGTCTTTGTG GTGCCTCATT CCCATAACGA CCCAGGTTGG
    TTGAAGACTT TCAATGACTA CTTTAGAGAC AAGACTCAGT ATATTTTTAA TAACATGGTC
    CTAAAGCTGA AAGAAGACTC ACGGAGGAAG TTTATTTGGT CTGAGATCTC TTACCTTTCA
    AAGTGGTGGG ATATTATAGA TATTCAGAAG AAGGATGCTG TTAAAAGTTT AATAGAAAAT
    GGTCAGCTTG AAATTGTGAC AGGTGGCTGG GTTATGCCTG ATGAAGCTAC TCCACATTAT
    TTTGCCTTAA TTGATCAACT AATTGAAGGA CATCAGTGGC TGGAAAATAA TATAGGAGTG
    AAACCTCGGT CCGGCTGGGC TATTGATCCC TTTGGACACT CACCAACAAT GGCTTATCTT
    CTAAACCGTG CTGGACTTTC TCACATGCTT ATCCAGAGAG TTCATTATGC AGTTAAAAAA
    CACTTTGCAC TGCATAAAAC ATTGGAGTTT TTTTGGAGAC AGAATTGGGA TCTGGGATCT
    GTCACAGATA TTTTATGCCA CATGATGCCC TTCTACAGCT ATGACATCCC TCACACTTGT
    GGACCTGATC CTAAAATATG CTGCCAGTTT GATTTTAAAC GTCTTCCTGG AGGCAGATTT
    GGTTGTCCCT GGGGAGTCCC CCCAGAAACA ATACATCCTG GAAATGTCCA AAGCAGGGCT
    CGGATGCTAC TAGATCAGTA CCGAAAGAAG TCAAAGCTTT TTCGTACCAA AGTTCTCCTG
    GCTCCACTAG GAGATGATTT CCGCTACTGT GAATACACGG AATGGGATTT ACAGTTTAAG
    AATTATCAGC AGCTTTTTGA TTATATGAAT TCTCAGTCCA AGTTTAAAGT TAAGATACAG
    TTTGGAACTT TATCAGATTT TTTTGATGCG CTGGATAAAG CAGATGAAAC TCAGAGAGAC
    AAGGGCCAAT CGATGTTCCC TGTTTTAAGT GGAGATTTTT TCACTTATGC CGATCGAGAT
    GATCATTACT GGAGTGGCTA TTTTACATCC AGACCCTTTT ACAAACGAAT GGACAGAATC
    ATGGAATCTC ATTTAAGGGC TGCTGAAATT CTTTACTATT TCGCCCTGAG ACAAGCTCAC
    AAATACAAGA TAAATAAATT TCTCTCATCA TCACTTTACA CGGCACTGAC AGAAGCCAGA
    AGGAATTTGG GACTGTTTCA ACATCATGAT GCTATCACAG GAACTGCAAA AGACTGGGTG
    GTTGTGGATT ATGGTACCAG ACTTTTTCAT TCGTTAATGG TTTTGGAGAA GATAATTGGA
    AATTCTGCAT TTCTTCTTAT TTTGAAGGAC AAACTCACAT ACGACTCTTA CTCTCCTGAT
    ACCTTCCTGG AGATGGATTT GAAACAAAAA TCACAAGATT CTCTGCCACA AAAAAATATA
    ATAAGGCTGA GTGCGGAGCC AAGGTACCTT GTGGTCTATA ATCCTTTAGA ACAAGACCGA
    ATCTCGTTGG TCTCAGTCTA TGTGAGTTCC CCGACAGTGC AAGTGTTCTC TGCTTCAGGA
    AAACCTGTGG AAGTTCAAGT CAGCGCAGTT TGGGATACAG CAAATACTAT TTCAGAAACA
    GCCTATGAGA TCTCTTTTCG AGCACATATA CCGCCATTGG GACTGAAAGT GTATAAGATT
    TTGGAATCAG CAAGTTCAAA TTCACATTTA GCTGATTATG TCTTGTATAA GAATAAAGTA
    GAAGATAGCG GAATTTTCAC CATAAAGAAT ATGATAAATA CTGAAGAAGG TATAACACTA
    GAGAACTCCT TTGTTTTACT TCGGTTTGAT CAAACTGGAC TTATGAAGCA AATGATGACT
    AAAGAAGATG GTAAACACCA TGAAGTAAAT GTGCAATTTT CATGGTATGG AACCACAATT
    AAAAGAGACA AAAGTGGTGC CTACCTCTTC TTACCTGATG GTAATGCCAA GCCTTATGTT
    TACACAACAC CGCCCTTTGT CAGAGTGACA CATGGAAGGA TTTATTCGGA AGTGACTTGC
    TTTTTTGACC ATGTTACTCA TAGAGTCCGA CTATACCACA TACAGGGCAT GAAAGAGAAG
    GTTATTTTAA GGGACTGCAA GCACATTCTG GTTACACAGA TTCAGAACTC AGAGGTTATC
    TTTGGGGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011541697.1
    CDS1002..3656
    Translation

    Target ORF information:

    RefSeq Version XM_011543395.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011543395.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAAGTTAA GCCGCCAGTT CACCGTGTTC GGCAGTGCGA TCTTCTGTGT GGTGATTTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTAGACTACC CCAGGAACCC GCGCCGCGAG
    GGCTCCTTCC CTCAGGGCCA GCTCTCAATG TTGCAAGAAA AAATAGACCA TTTGGAGCGT
    TTGCTAGCTG AGAATAATGA GATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT
    GAGTCTGTGG AGGATGGTCC GAAAAGTTCA CAAAGCAATT TCAGCCAAGG TGCTGGCTCA
    CATCTTCTGC CCTCACAATT ATCCCTCTCA GTTGACACTG CAGACTGTCT GTTTGCTTCA
    CAAAGTGGAA GTCACAATTC AGATGTGCAG ATGTTGGATG TTTACAGTCT AATTTCTTTT
    GACAATCCAG ATGGTGGAGT TTGGAAGCAA GGATTTGACA TTACTTATGA ATCTAATGAA
    TGGGACACTG AACCCCTTCA AGTCTTTGTG GTGCCTCATT CCCATAACGA CCCAGGTTGG
    TTGAAGACTT TCAATGACTA CTTTAGAGAC AAGACTCAGT ATATTTTTAA TAACATGGTC
    CTAAAGCTGA AAGAAGACTC ACGGAGGAAG TTTATTTGGT CTGAGATCTC TTACCTTTCA
    AAGTGGTGGG ATATTATAGA TATTCAGAAG AAGGATGCTG TTAAAAGTTT AATAGAAAAT
    GGTCAGCTTG AAATTGTGAC AGGTGGCTGG GTTATGCCTG ATGAAGCTAC TCCACATTAT
    TTTGCCTTAA TTGATCAACT AATTGAAGGA CATCAGTGGC TGGAAAATAA TATAGGAGTG
    AAACCTCGGT CCGGCTGGGC TATTGATCCC TTTGGACACT CACCAACAAT GGCTTATCTT
    CTAAACCGTG CTGGACTTTC TCACATGCTT ATCCAGAGAG TTCATTATGC AGTTAAAAAA
    CACTTTGCAC TGCATAAAAC ATTGGAGTTT TTTTGGAGAC AGAATTGGGA TCTGGGATCT
    GTCACAGATA TTTTATGCCA CATGATGCCC TTCTACAGCT ATGACATCCC TCACACTTGT
    GGACCTGATC CTAAAATATG CTGCCAGTTT GATTTTAAAC GTCTTCCTGG AGGCAGATTT
    GGTTGTCCCT GGGGAGTCCC CCCAGAAACA ATACATCCTG GAAATGTCCA AAGCAGGGCT
    CGGATGCTAC TAGATCAGTA CCGAAAGAAG TCAAAGCTTT TTCGTACCAA AGTTCTCCTG
    GCTCCACTAG GAGATGATTT CCGCTACTGT GAATACACGG AATGGGATTT ACAGTTTAAG
    AATTATCAGC AGCTTTTTGA TTATATGAAT TCTCAGTCCA AGTTTAAAGT TAAGATACAG
    TTTGGAACTT TATCAGATTT TTTTGATGCG CTGGATAAAG CAGATGAAAC TCAGAGAGAC
    AAGGGCCAAT CGATGTTCCC TGTTTTAAGT GGAGATTTTT TCACTTATGC CGATCGAGAT
    GATCATTACT GGAGTGGCTA TTTTACATCC AGACCCTTTT ACAAACGAAT GGACAGAATC
    ATGGAATCTC ATTTAAGGGC TGCTGAAATT CTTTACTATT TCGCCCTGAG ACAAGCTCAC
    AAATACAAGA TAAATAAATT TCTCTCATCA TCACTTTACA CGGCACTGAC AGAAGCCAGA
    AGGAATTTGG GACTGTTTCA ACATCATGAT GCTATCACAG GAACTGCAAA AGACTGGGTG
    GTTGTGGATT ATGGTACCAG ACTTTTTCAT TCGTTAATGG TTTTGGAGAA GATAATTGGA
    AATTCTGCAT TTCTTCTTAT TTTGAAGGAC AAACTCACAT ACGACTCTTA CTCTCCTGAT
    ACCTTCCTGG AGATGGATTT GAAACAAAAA TCACAAGATT CTCTGCCACA AAAAAATATA
    ATAAGGCTGA GTGCGGAGCC AAGGTACCTT GTGGTCTATA ATCCTTTAGA ACAAGACCGA
    ATCTCGTTGG TCTCAGTCTA TGTGAGTTCC CCGACAGTGC AAGTGTTCTC TGCTTCAGGA
    AAACCTGTGG AAGTTCAAGT CAGCGCAGTT TGGGATACAG CAAATACTAT TTCAGAAACA
    GCCTATGAGA TCTCTTTTCG AGCACATATA CCGCCATTGG GACTGAAAGT GTATAAGATT
    TTGGAATCAG CAAGTTCAAA TTCACATTTA GCTGATTATG TCTTGTATAA GAATAAAGTA
    GAAGATAGCG GAATTTTCAC CATAAAGAAT ATGATAAATA CTGAAGAAGG TATAACACTA
    GAGAACTCCT TTGTTTTACT TCGGTTTGAT CAAACTGGAC TTATGAAGCA AATGATGACT
    AAAGAAGATG GTAAACACCA TGAAGTAAAT GTGCAATTTT CATGGTATGG AACCACAATT
    AAAAGAGACA AAAGTGGTGC CTACCTCTTC TTACCTGATG GTAATGCCAA GCCTTATGTT
    TACACAACAC CGCCCTTTGT CAGAGTGACA CATGGAAGGA TTTATTCGGA AGTGACTTGC
    TTTTTTGACC ATGTTACTCA TAGAGTCCGA CTATACCACA TACAGGGCAT GAAAGAGAAG
    GTTATTTTAA GGGACTGCAA GCACATTCTG GTTACACAGA TTCAGAACTC AGAGGTTATC
    TTTGGGGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105666
    Clone ID Related Accession (Same CDS sequence) XM_024446048.1
    Accession Version XM_024446048.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2841bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGTCCTAA AGCTGAAAGA AGACTCACGG AGGAAGTTTA TTTGGTCTGA GATCTCTTAC 
    CTTTCAAAGT GGTGGGATAT TATAGATATT CAGAAGAAGG ATGCTGTTAA AAGTTTAATA
    GAAAATGGTC AGCTTGAAAT TGTGACAGGT GGCTGGGTTA TGCCTGATGA AGCTACTCCA
    CATTATTTTG CCTTAATTGA TCAACTAATT GAAGGACATC AGTGGCTGGA AAATAATATA
    GGAGTGAAAC CTCGGTCCGG CTGGGCTATT GATCCCTTTG GACACTCACC AACAATGGCT
    TATCTTCTAA ACCGTGCTGG ACTTTCTCAC ATGCTTATCC AGAGAGTTCA TTATGCAGTT
    AAAAAACACT TTGCACTGCA TAAAACATTG GAGTTTTTTT GGAGACAGAA TTGGGATCTG
    GGATCTGTCA CAGATATTTT ATGCCACATG ATGCCCTTCT ACAGCTATGA CATCCCTCAC
    ACTTGTGGAC CTGATCCTAA AATATGCTGC CAGTTTGATT TTAAACGTCT TCCTGGAGGC
    AGATTTGGTT GTCCCTGGGG AGTCCCCCCA GAAACAATAC ATCCTGGAAA TGTCCAAAGC
    AGGGCTCGGA TGCTACTAGA TCAGTACCGA AAGAAGTCAA AGCTTTTTCG TACCAAAGTT
    CTCCTGGCTC CACTAGGAGA TGATTTCCGC TACTGTGAAT ACACGGAATG GGATTTACAG
    TTTAAGAATT ATCAGCAGCT TTTTGATTAT ATGAATTCTC AGTCCAAGTT TAAAGTTAAG
    ATACAGTTTG GAACTTTATC AGATTTTTTT GATGCGCTGG ATAAAGCAGA TGAAACTCAG
    AGAGACAAGG GCCAATCGAT GTTCCCTGTT TTAAGTGGAG ATTTTTTCAC TTATGCCGAT
    CGAGATGATC ATTACTGGAG TGGCTATTTT ACATCCAGAC CCTTTTACAA ACGAATGGAC
    AGAATCATGG AATCTCATTT AAGGGCTGCT GAAATTCTTT ACTATTTCGC CCTGAGACAA
    GCTCACAAAT ACAAGATAAA TAAATTTCTC TCATCATCAC TTTACACGGC ACTGACAGAA
    GCCAGAAGGA ATTTGGGACT GTTTCAACAT CATGATGCTA TCACAGGAAC TGCAAAAGAC
    TGGGTGGTTG TGGATTATGG TACCAGACTT TTTCATTCGT TAATGGTTTT GGAGAAGATA
    ATTGGAAATT CTGCATTTCT TCTTATTTTG AAGGACAAAC TCACATACGA CTCTTACTCT
    CCTGATACCT TCCTGGAGAT GGATTTGAAA CAAAAATCAC AAGATTCTCT GCCACAAAAA
    AATATAATAA GGCTGAGTGC GGAGCCAAGG TACCTTGTGG TCTATAATCC TTTAGAACAA
    GACCGAATCT CGTTGGTCTC AGTCTATGTG AGTTCCCCGA CAGTGCAAGT GTTCTCTGCT
    TCAGGAAAAC CTGTGGAAGT TCAAGTCAGC GCAGTTTGGG ATACAGCAAA TACTATTTCA
    GAAACAGCCT ATGAGATCTC TTTTCGAGCA CATATACCGC CATTGGGACT GAAAGTGTAT
    AAGATTTTGG AATCAGCAAG TTCAAATTCA CATTTAGCTG ATTATGTCTT GTATAAGAAT
    AAAGTAGAAG ATAGCGGAAT TTTCACCATA AAGAATATGA TAAATACTGA AGAAGGTATA
    ACACTAGAGA ACTCCTTTGT TTTACTTCGG TTTGATCAAA CTGGACTTAT GAAGCAAATG
    ATGACTAAAG AAGATGGTAA ACACCATGAA GTAAATGTGC AATTTTCATG GTATGGAACC
    ACAATTAAAA GAGACAAAAG TGGTGCCTAC CTCTTCTTAC CTGATGGTAA TGCCAAGCCT
    TATGTTTACA CAACACCGCC CTTTGTCAGA GTGACACATG GAAGGATTTA TTCGGAAGTG
    ACTTGCTTTT TTGACCATGT TACTCATAGA GTCCGACTAT ACCACATACA GGGAATAGAA
    GGACAGTCTG TGGAAGTTTC CAATATTGTG GACATCCGAA AAGTATATAA CCGTGAGATT
    GCAATGAAAA TTTCTTCTGA TATAAAAAGC CAAAATAGAT TTTATACTGA CCTAAATGGG
    TACCAGATTC AACCTAGAAT GACACTGAGC AAATTGCCTC TTCAAGCAAA TGTCTATCCC
    ATGACCACAA TGGCCTATAT CCAGGATGCC AAACATCGTT TGACACTGCT CTCTGCTCAG
    TCATTAGGGG TTTCGAGTTT GAATAGTGGT CAGATTGAAG TTATCATGGA TCGAAGACTC
    ATGCAAGATG ATAATCGTGG CCTTGAGCAA GGTATCCAGG ATAACAAGAT TACAGCTAAT
    CTATTTCGAA TACTACTAGA AAAAAGAAGT GCTGTTAATA CGGAAGAAGA AAAGAAGTCG
    GTCAGTTATC CTTCTCTCCT TAGCCACATA ACTTCTTCTC TCATGAATCA TCCAGTCATT
    CCAATGGCAA ATAAGTTCTC CTCACCTACC CTTGAGCTGC AAGGTGAATT CTCTCCATTA
    CAGTCATCTT TGCCTTGTGA CATTCATCTG GTTAATTTGA GAACAATACA GTCAAAGGTG
    GGCAATGGGC ACTCCAATGA GGCAGCCTTG ATCCTCCACA GAAAAGGGTT TGATTGTCGG
    TTCTCTAGCA AAGGCACAGG GCTGTTTTGT TCTACTACTC AGGGAAAGAT ATTGGTACAG
    AAACTTTTAA ACAAGTTTAT TGTCGAAAGT CTCACACCTT CATCACTATC CTTGATGCAT
    TCACCTCCCG GCACTCAGAA TATAAGTGAG ATCAACTTGA GTCCAATGGA AATCAGCACA
    TTCCGAATCC AGTTGAGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024301816.1
    CDS1556..4396
    Translation

    Target ORF information:

    RefSeq Version XM_024446048.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024446048.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGTCCTAA AGCTGAAAGA AGACTCACGG AGGAAGTTTA TTTGGTCTGA GATCTCTTAC 
    CTTTCAAAGT GGTGGGATAT TATAGATATT CAGAAGAAGG ATGCTGTTAA AAGTTTAATA
    GAAAATGGTC AGCTTGAAAT TGTGACAGGT GGCTGGGTTA TGCCTGATGA AGCTACTCCA
    CATTATTTTG CCTTAATTGA TCAACTAATT GAAGGACATC AGTGGCTGGA AAATAATATA
    GGAGTGAAAC CTCGGTCCGG CTGGGCTATT GATCCCTTTG GACACTCACC AACAATGGCT
    TATCTTCTAA ACCGTGCTGG ACTTTCTCAC ATGCTTATCC AGAGAGTTCA TTATGCAGTT
    AAAAAACACT TTGCACTGCA TAAAACATTG GAGTTTTTTT GGAGACAGAA TTGGGATCTG
    GGATCTGTCA CAGATATTTT ATGCCACATG ATGCCCTTCT ACAGCTATGA CATCCCTCAC
    ACTTGTGGAC CTGATCCTAA AATATGCTGC CAGTTTGATT TTAAACGTCT TCCTGGAGGC
    AGATTTGGTT GTCCCTGGGG AGTCCCCCCA GAAACAATAC ATCCTGGAAA TGTCCAAAGC
    AGGGCTCGGA TGCTACTAGA TCAGTACCGA AAGAAGTCAA AGCTTTTTCG TACCAAAGTT
    CTCCTGGCTC CACTAGGAGA TGATTTCCGC TACTGTGAAT ACACGGAATG GGATTTACAG
    TTTAAGAATT ATCAGCAGCT TTTTGATTAT ATGAATTCTC AGTCCAAGTT TAAAGTTAAG
    ATACAGTTTG GAACTTTATC AGATTTTTTT GATGCGCTGG ATAAAGCAGA TGAAACTCAG
    AGAGACAAGG GCCAATCGAT GTTCCCTGTT TTAAGTGGAG ATTTTTTCAC TTATGCCGAT
    CGAGATGATC ATTACTGGAG TGGCTATTTT ACATCCAGAC CCTTTTACAA ACGAATGGAC
    AGAATCATGG AATCTCATTT AAGGGCTGCT GAAATTCTTT ACTATTTCGC CCTGAGACAA
    GCTCACAAAT ACAAGATAAA TAAATTTCTC TCATCATCAC TTTACACGGC ACTGACAGAA
    GCCAGAAGGA ATTTGGGACT GTTTCAACAT CATGATGCTA TCACAGGAAC TGCAAAAGAC
    TGGGTGGTTG TGGATTATGG TACCAGACTT TTTCATTCGT TAATGGTTTT GGAGAAGATA
    ATTGGAAATT CTGCATTTCT TCTTATTTTG AAGGACAAAC TCACATACGA CTCTTACTCT
    CCTGATACCT TCCTGGAGAT GGATTTGAAA CAAAAATCAC AAGATTCTCT GCCACAAAAA
    AATATAATAA GGCTGAGTGC GGAGCCAAGG TACCTTGTGG TCTATAATCC TTTAGAACAA
    GACCGAATCT CGTTGGTCTC AGTCTATGTG AGTTCCCCGA CAGTGCAAGT GTTCTCTGCT
    TCAGGAAAAC CTGTGGAAGT TCAAGTCAGC GCAGTTTGGG ATACAGCAAA TACTATTTCA
    GAAACAGCCT ATGAGATCTC TTTTCGAGCA CATATACCGC CATTGGGACT GAAAGTGTAT
    AAGATTTTGG AATCAGCAAG TTCAAATTCA CATTTAGCTG ATTATGTCTT GTATAAGAAT
    AAAGTAGAAG ATAGCGGAAT TTTCACCATA AAGAATATGA TAAATACTGA AGAAGGTATA
    ACACTAGAGA ACTCCTTTGT TTTACTTCGG TTTGATCAAA CTGGACTTAT GAAGCAAATG
    ATGACTAAAG AAGATGGTAA ACACCATGAA GTAAATGTGC AATTTTCATG GTATGGAACC
    ACAATTAAAA GAGACAAAAG TGGTGCCTAC CTCTTCTTAC CTGATGGTAA TGCCAAGCCT
    TATGTTTACA CAACACCGCC CTTTGTCAGA GTGACACATG GAAGGATTTA TTCGGAAGTG
    ACTTGCTTTT TTGACCATGT TACTCATAGA GTCCGACTAT ACCACATACA GGGAATAGAA
    GGACAGTCTG TGGAAGTTTC CAATATTGTG GACATCCGAA AAGTATATAA CCGTGAGATT
    GCAATGAAAA TTTCTTCTGA TATAAAAAGC CAAAATAGAT TTTATACTGA CCTAAATGGG
    TACCAGATTC AACCTAGAAT GACACTGAGC AAATTGCCTC TTCAAGCAAA TGTCTATCCC
    ATGACCACAA TGGCCTATAT CCAGGATGCC AAACATCGTT TGACACTGCT CTCTGCTCAG
    TCATTAGGGG TTTCGAGTTT GAATAGTGGT CAGATTGAAG TTATCATGGA TCGAAGACTC
    ATGCAAGATG ATAATCGTGG CCTTGAGCAA GGTATCCAGG ATAACAAGAT TACAGCTAAT
    CTATTTCGAA TACTACTAGA AAAAAGAAGT GCTGTTAATA CGGAAGAAGA AAAGAAGTCG
    GTCAGTTATC CTTCTCTCCT TAGCCACATA ACTTCTTCTC TCATGAATCA TCCAGTCATT
    CCAATGGCAA ATAAGTTCTC CTCACCTACC CTTGAGCTGC AAGGTGAATT CTCTCCATTA
    CAGTCATCTT TGCCTTGTGA CATTCATCTG GTTAATTTGA GAACAATACA GTCAAAGGTG
    GGCAATGGGC ACTCCAATGA GGCAGCCTTG ATCCTCCACA GAAAAGGGTT TGATTGTCGG
    TTCTCTAGCA AAGGCACAGG GCTGTTTTGT TCTACTACTC AGGGAAAGAT ATTGGTACAG
    AAACTTTTAA ACAAGTTTAT TGTCGAAAGT CTCACACCTT CATCACTATC CTTGATGCAT
    TCACCTCCCG GCACTCAGAA TATAAGTGAG ATCAACTTGA GTCCAATGGA AATCAGCACA
    TTCCGAATCC AGTTGAGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10839
    Clone ID Related Accession (Same CDS sequence) NM_002372.3
    Accession Version NM_002372.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3435bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-04
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012603.7, BC111062.1, U31520.1, D63998.1, AC011366.6 and AL833486.1. On Dec 19, 2013 this sequence version replaced NM_002372.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D63998.1, SRR1803617.70937.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGTTAA GCCGCCAGTT CACCGTGTTC GGCAGTGCGA TCTTCTGTGT GGTGATTTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTAGACTACC CCAGGAACCC GCGCCGCGAG
    GGCTCCTTCC CTCAGGGCCA GCTCTCAATG TTGCAAGAAA AAATAGACCA TTTGGAGCGT
    TTGCTAGCTG AGAATAATGA GATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT
    GAGTCTGTGG AGGATGGTCC GAAAAGTTCA CAAAGCAATT TCAGCCAAGG TGCTGGCTCA
    CATCTTCTGC CCTCACAATT ATCCCTCTCA GTTGACACTG CAGACTGTCT GTTTGCTTCA
    CAAAGTGGAA GTCACAATTC AGATGTGCAG ATGTTGGATG TTTACAGTCT AATTTCTTTT
    GACAATCCAG ATGGTGGAGT TTGGAAGCAA GGATTTGACA TTACTTATGA ATCTAATGAA
    TGGGACACTG AACCCCTTCA AGTCTTTGTG GTGCCTCATT CCCATAACGA CCCAGGTTGG
    TTGAAGACTT TCAATGACTA CTTTAGAGAC AAGACTCAGT ATATTTTTAA TAACATGGTC
    CTAAAGCTGA AAGAAGACTC ACGGAGGAAG TTTATTTGGT CTGAGATCTC TTACCTTTCA
    AAGTGGTGGG ATATTATAGA TATTCAGAAG AAGGATGCTG TTAAAAGTTT AATAGAAAAT
    GGTCAGCTTG AAATTGTGAC AGGTGGCTGG GTTATGCCTG ATGAAGCTAC TCCACATTAT
    TTTGCCTTAA TTGATCAACT AATTGAAGGA CATCAGTGGC TGGAAAATAA TATAGGAGTG
    AAACCTCGGT CCGGCTGGGC TATTGATCCC TTTGGACACT CACCAACAAT GGCTTATCTT
    CTAAACCGTG CTGGACTTTC TCACATGCTT ATCCAGAGAG TTCATTATGC AGTTAAAAAA
    CACTTTGCAC TGCATAAAAC ATTGGAGTTT TTTTGGAGAC AGAATTGGGA TCTGGGATCT
    GTCACAGATA TTTTATGCCA CATGATGCCC TTCTACAGCT ATGACATCCC TCACACTTGT
    GGACCTGATC CTAAAATATG CTGCCAGTTT GATTTTAAAC GTCTTCCTGG AGGCAGATTT
    GGTTGTCCCT GGGGAGTCCC CCCAGAAACA ATACATCCTG GAAATGTCCA AAGCAGGGCT
    CGGATGCTAC TAGATCAGTA CCGAAAGAAG TCAAAGCTTT TTCGTACCAA AGTTCTCCTG
    GCTCCACTAG GAGATGATTT CCGCTACTGT GAATACACGG AATGGGATTT ACAGTTTAAG
    AATTATCAGC AGCTTTTTGA TTATATGAAT TCTCAGTCCA AGTTTAAAGT TAAGATACAG
    TTTGGAACTT TATCAGATTT TTTTGATGCG CTGGATAAAG CAGATGAAAC TCAGAGAGAC
    AAGGGCCAAT CGATGTTCCC TGTTTTAAGT GGAGATTTTT TCACTTATGC CGATCGAGAT
    GATCATTACT GGAGTGGCTA TTTTACATCC AGACCCTTTT ACAAACGAAT GGACAGAATC
    ATGGAATCTC ATTTAAGGGC TGCTGAAATT CTTTACTATT TCGCCCTGAG ACAAGCTCAC
    AAATACAAGA TAAATAAATT TCTCTCATCA TCACTTTACA CGGCACTGAC AGAAGCCAGA
    AGGAATTTGG GACTGTTTCA ACATCATGAT GCTATCACAG GAACTGCAAA AGACTGGGTG
    GTTGTGGATT ATGGTACCAG ACTTTTTCAT TCGTTAATGG TTTTGGAGAA GATAATTGGA
    AATTCTGCAT TTCTTCTTAT TTTGAAGGAC AAACTCACAT ACGACTCTTA CTCTCCTGAT
    ACCTTCCTGG AGATGGATTT GAAACAAAAA TCACAAGATT CTCTGCCACA AAAAAATATA
    ATAAGGCTGA GTGCGGAGCC AAGGTACCTT GTGGTCTATA ATCCTTTAGA ACAAGACCGA
    ATCTCGTTGG TCTCAGTCTA TGTGAGTTCC CCGACAGTGC AAGTGTTCTC TGCTTCAGGA
    AAACCTGTGG AAGTTCAAGT CAGCGCAGTT TGGGATACAG CAAATACTAT TTCAGAAACA
    GCCTATGAGA TCTCTTTTCG AGCACATATA CCGCCATTGG GACTGAAAGT GTATAAGATT
    TTGGAATCAG CAAGTTCAAA TTCACATTTA GCTGATTATG TCTTGTATAA GAATAAAGTA
    GAAGATAGCG GAATTTTCAC CATAAAGAAT ATGATAAATA CTGAAGAAGG TATAACACTA
    GAGAACTCCT TTGTTTTACT TCGGTTTGAT CAAACTGGAC TTATGAAGCA AATGATGACT
    AAAGAAGATG GTAAACACCA TGAAGTAAAT GTGCAATTTT CATGGTATGG AACCACAATT
    AAAAGAGACA AAAGTGGTGC CTACCTCTTC TTACCTGATG GTAATGCCAA GCCTTATGTT
    TACACAACAC CGCCCTTTGT CAGAGTGACA CATGGAAGGA TTTATTCGGA AGTGACTTGC
    TTTTTTGACC ATGTTACTCA TAGAGTCCGA CTATACCACA TACAGGGAAT AGAAGGACAG
    TCTGTGGAAG TTTCCAATAT TGTGGACATC CGAAAAGTAT ATAACCGTGA GATTGCAATG
    AAAATTTCTT CTGATATAAA AAGCCAAAAT AGATTTTATA CTGACCTAAA TGGGTACCAG
    ATTCAACCTA GAATGACACT GAGCAAATTG CCTCTTCAAG CAAATGTCTA TCCCATGACC
    ACAATGGCCT ATATCCAGGA TGCCAAACAT CGTTTGACAC TGCTCTCTGC TCAGTCATTA
    GGGGTTTCGA GTTTGAATAG TGGTCAGATT GAAGTTATCA TGGATCGAAG ACTCATGCAA
    GATGATAATC GTGGCCTTGA GCAAGGTATC CAGGATAACA AGATTACAGC TAATCTATTT
    CGAATACTAC TAGAAAAAAG AAGTGCTGTT AATACGGAAG AAGAAAAGAA GTCGGTCAGT
    TATCCTTCTC TCCTTAGCCA CATAACTTCT TCTCTCATGA ATCATCCAGT CATTCCAATG
    GCAAATAAGT TCTCCTCACC TACCCTTGAG CTGCAAGGTG AATTCTCTCC ATTACAGTCA
    TCTTTGCCTT GTGACATTCA TCTGGTTAAT TTGAGAACAA TACAGTCAAA GGTGGGCAAT
    GGGCACTCCA ATGAGGCAGC CTTGATCCTC CACAGAAAAG GGTTTGATTG TCGGTTCTCT
    AGCAAAGGCA CAGGGCTGTT TTGTTCTACT ACTCAGGGAA AGATATTGGT ACAGAAACTT
    TTAAACAAGT TTATTGTCGA AAGTCTCACA CCTTCATCAC TATCCTTGAT GCATTCACCT
    CCCGGCACTC AGAATATAAG TGAGATCAAC TTGAGTCCAA TGGAAATCAG CACATTCCGA
    ATCCAGTTGA GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002363.2
    CDS1053..4487
    Translation

    Target ORF information:

    RefSeq Version NM_002372.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 1 (MAN2A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002372.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGTTAA GCCGCCAGTT CACCGTGTTC GGCAGTGCGA TCTTCTGTGT GGTGATTTTC 
    TCGCTCTACC TGATGCTGGA CCGGGGTCAC TTAGACTACC CCAGGAACCC GCGCCGCGAG
    GGCTCCTTCC CTCAGGGCCA GCTCTCAATG TTGCAAGAAA AAATAGACCA TTTGGAGCGT
    TTGCTAGCTG AGAATAATGA GATCATCTCA AATATTAGAG ACTCAGTCAT CAATTTGAGT
    GAGTCTGTGG AGGATGGTCC GAAAAGTTCA CAAAGCAATT TCAGCCAAGG TGCTGGCTCA
    CATCTTCTGC CCTCACAATT ATCCCTCTCA GTTGACACTG CAGACTGTCT GTTTGCTTCA
    CAAAGTGGAA GTCACAATTC AGATGTGCAG ATGTTGGATG TTTACAGTCT AATTTCTTTT
    GACAATCCAG ATGGTGGAGT TTGGAAGCAA GGATTTGACA TTACTTATGA ATCTAATGAA
    TGGGACACTG AACCCCTTCA AGTCTTTGTG GTGCCTCATT CCCATAACGA CCCAGGTTGG
    TTGAAGACTT TCAATGACTA CTTTAGAGAC AAGACTCAGT ATATTTTTAA TAACATGGTC
    CTAAAGCTGA AAGAAGACTC ACGGAGGAAG TTTATTTGGT CTGAGATCTC TTACCTTTCA
    AAGTGGTGGG ATATTATAGA TATTCAGAAG AAGGATGCTG TTAAAAGTTT AATAGAAAAT
    GGTCAGCTTG AAATTGTGAC AGGTGGCTGG GTTATGCCTG ATGAAGCTAC TCCACATTAT
    TTTGCCTTAA TTGATCAACT AATTGAAGGA CATCAGTGGC TGGAAAATAA TATAGGAGTG
    AAACCTCGGT CCGGCTGGGC TATTGATCCC TTTGGACACT CACCAACAAT GGCTTATCTT
    CTAAACCGTG CTGGACTTTC TCACATGCTT ATCCAGAGAG TTCATTATGC AGTTAAAAAA
    CACTTTGCAC TGCATAAAAC ATTGGAGTTT TTTTGGAGAC AGAATTGGGA TCTGGGATCT
    GTCACAGATA TTTTATGCCA CATGATGCCC TTCTACAGCT ATGACATCCC TCACACTTGT
    GGACCTGATC CTAAAATATG CTGCCAGTTT GATTTTAAAC GTCTTCCTGG AGGCAGATTT
    GGTTGTCCCT GGGGAGTCCC CCCAGAAACA ATACATCCTG GAAATGTCCA AAGCAGGGCT
    CGGATGCTAC TAGATCAGTA CCGAAAGAAG TCAAAGCTTT TTCGTACCAA AGTTCTCCTG
    GCTCCACTAG GAGATGATTT CCGCTACTGT GAATACACGG AATGGGATTT ACAGTTTAAG
    AATTATCAGC AGCTTTTTGA TTATATGAAT TCTCAGTCCA AGTTTAAAGT TAAGATACAG
    TTTGGAACTT TATCAGATTT TTTTGATGCG CTGGATAAAG CAGATGAAAC TCAGAGAGAC
    AAGGGCCAAT CGATGTTCCC TGTTTTAAGT GGAGATTTTT TCACTTATGC CGATCGAGAT
    GATCATTACT GGAGTGGCTA TTTTACATCC AGACCCTTTT ACAAACGAAT GGACAGAATC
    ATGGAATCTC ATTTAAGGGC TGCTGAAATT CTTTACTATT TCGCCCTGAG ACAAGCTCAC
    AAATACAAGA TAAATAAATT TCTCTCATCA TCACTTTACA CGGCACTGAC AGAAGCCAGA
    AGGAATTTGG GACTGTTTCA ACATCATGAT GCTATCACAG GAACTGCAAA AGACTGGGTG
    GTTGTGGATT ATGGTACCAG ACTTTTTCAT TCGTTAATGG TTTTGGAGAA GATAATTGGA
    AATTCTGCAT TTCTTCTTAT TTTGAAGGAC AAACTCACAT ACGACTCTTA CTCTCCTGAT
    ACCTTCCTGG AGATGGATTT GAAACAAAAA TCACAAGATT CTCTGCCACA AAAAAATATA
    ATAAGGCTGA GTGCGGAGCC AAGGTACCTT GTGGTCTATA ATCCTTTAGA ACAAGACCGA
    ATCTCGTTGG TCTCAGTCTA TGTGAGTTCC CCGACAGTGC AAGTGTTCTC TGCTTCAGGA
    AAACCTGTGG AAGTTCAAGT CAGCGCAGTT TGGGATACAG CAAATACTAT TTCAGAAACA
    GCCTATGAGA TCTCTTTTCG AGCACATATA CCGCCATTGG GACTGAAAGT GTATAAGATT
    TTGGAATCAG CAAGTTCAAA TTCACATTTA GCTGATTATG TCTTGTATAA GAATAAAGTA
    GAAGATAGCG GAATTTTCAC CATAAAGAAT ATGATAAATA CTGAAGAAGG TATAACACTA
    GAGAACTCCT TTGTTTTACT TCGGTTTGAT CAAACTGGAC TTATGAAGCA AATGATGACT
    AAAGAAGATG GTAAACACCA TGAAGTAAAT GTGCAATTTT CATGGTATGG AACCACAATT
    AAAAGAGACA AAAGTGGTGC CTACCTCTTC TTACCTGATG GTAATGCCAA GCCTTATGTT
    TACACAACAC CGCCCTTTGT CAGAGTGACA CATGGAAGGA TTTATTCGGA AGTGACTTGC
    TTTTTTGACC ATGTTACTCA TAGAGTCCGA CTATACCACA TACAGGGAAT AGAAGGACAG
    TCTGTGGAAG TTTCCAATAT TGTGGACATC CGAAAAGTAT ATAACCGTGA GATTGCAATG
    AAAATTTCTT CTGATATAAA AAGCCAAAAT AGATTTTATA CTGACCTAAA TGGGTACCAG
    ATTCAACCTA GAATGACACT GAGCAAATTG CCTCTTCAAG CAAATGTCTA TCCCATGACC
    ACAATGGCCT ATATCCAGGA TGCCAAACAT CGTTTGACAC TGCTCTCTGC TCAGTCATTA
    GGGGTTTCGA GTTTGAATAG TGGTCAGATT GAAGTTATCA TGGATCGAAG ACTCATGCAA
    GATGATAATC GTGGCCTTGA GCAAGGTATC CAGGATAACA AGATTACAGC TAATCTATTT
    CGAATACTAC TAGAAAAAAG AAGTGCTGTT AATACGGAAG AAGAAAAGAA GTCGGTCAGT
    TATCCTTCTC TCCTTAGCCA CATAACTTCT TCTCTCATGA ATCATCCAGT CATTCCAATG
    GCAAATAAGT TCTCCTCACC TACCCTTGAG CTGCAAGGTG AATTCTCTCC ATTACAGTCA
    TCTTTGCCTT GTGACATTCA TCTGGTTAAT TTGAGAACAA TACAGTCAAA GGTGGGCAAT
    GGGCACTCCA ATGAGGCAGC CTTGATCCTC CACAGAAAAG GGTTTGATTG TCGGTTCTCT
    AGCAAAGGCA CAGGGCTGTT TTGTTCTACT ACTCAGGGAA AGATATTGGT ACAGAAACTT
    TTAAACAAGT TTATTGTCGA AAGTCTCACA CCTTCATCAC TATCCTTGAT GCATTCACCT
    CCCGGCACTC AGAATATAAG TGAGATCAAC TTGAGTCCAA TGGAAATCAG CACATTCCGA
    ATCCAGTTGA GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58845
    Clone ID Related Accession (Same CDS sequence) XM_017009472.1
    Accession Version XM_017009472.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alpha-mannosidase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGCAAG AAAAAATAGA CCATTTGGAG CGTTTGCTAG CTGAGAATAA TGAGATCATC 
    TCAAATATTA GAGACTCAGT CATCAATTTG AGTGAGTCTG TGGAGGATGG TCCGAAAAGT
    TCACAAAGCA ATTTCAGCCA AGGTGCTGGC TCACATCTTC TGCCCTCACA ATTATCCCTC
    TCAGTTGACA CTGCAGACTG TCTGTTTGCT TCACAAAGTG GAAGTCACAA TTCAGATGTG
    CAGATGTTGG ATGTTTACAG TCTAATTTCT TTTGACAATC CAGATGGTGG AGTTTGGAAG
    CAAGGATTTG ACATTACTTA TGAATCTAAT GAATGGGACA CTGAACCCCT TCAAGTCTTT
    GTGGTGCCTC ATTCCCATAA CGACCCAGGT TGGTTGAAGA CTTTCAATGA CTACTTTAGA
    GACAAGACTC AGTATATTTT TAATAACATG GTCCTAAAGC TGAAAGAAGA CTCACGGAGG
    AAGTTTATTT GGTCTGAGAT CTCTTACCTT TCAAAGTGGT GGGATATTAT AGATATTCAG
    AAGAAGGATG CTGTTAAAAG TTTAATAGAA AATGGTCAGC TTGAAATTGT GACAGGTGGC
    TGGGTTATGC CTGATGAAGC TACTCCACAT TATTTTGCCT TAATTGATCA ACTAATTGAA
    GGACATCAGT GGCTGGAAAA TAATATAGGA GTGAAACCTC GGTCCGGCTG GGCTATTGAT
    CCCTTTGGAC ACTCACCAAC AATGGCTTAT CTTCTAAACC GTGCTGGACT TTCTCACATG
    CTTATCCAGA GAGTTCATTA TGCAGTTAAA AAACACTTTG CACTGCATAA AACATTGGAG
    TTTTTTTGGA GACAGAATTG GGATCTGGGA TCTGTCACAG ATATTTTATG CCACATGATG
    CCCTTCTACA GCTATGACAT CCCTCACACT TGTGGACCTG ATCCTAAAAT ATGCTGCCAG
    TTTGATTTTA AACGTCTTCC TGGAGGCAGA TTTGGTTGTC CCTGGGGAGT CCCCCCAGAA
    ACAATACATC CTGGAAATGT CCAAAGCAGG GCTCGGATGC TACTAGATCA GTACCGAAAG
    AAGTCAAAGC TTTTTCGTAC CAAAGTTCTC CTGGCTCCAC TAGGAGATGA TTTCCGCTAC
    TGTGAATACA CGGAATGGGA TTTACAGTTT AAGAATTATC AGCAGCTTTT TGATTATATG
    AATTCTCAGT CCAAGTTTAA AGTTAAGATA CAGTTTGGAA CTTTATCAGA TTTTTTTGAT
    GCGCTGGATA AAGCAGATGA AACTCAGAGA GACAAGGGCC AATCGATGTT CCCTGTTTTA
    AGTGGAGATT TTTTCACTTA TGCCGATCGA GATGATCATT ACTGGAGTGG CTATTTTACA
    TCCAGACCCT TTTACAAACG AATGGACAGA ATCATGGAAT CTCATTTAAG GGCTGCTGAA
    ATTCTTTACT ATTTCGCCCT GAGACAAGCT CACAAATACA AGATAAATAA ATTTCTCTCA
    TCATCACTTT ACACGGCACT GACAGAAGCC AGAAGGAATT TGGGACTGTT TCAACATCAT
    GATGCTATCA CAGGAACTGC AAAAGACTGG GTGGTTGTGG ATTATGGTAC CAGACTTTTT
    CATTCGTTAA TGGTTTTGGA GAAGATAATT GGAAATTCTG CATTTCTTCT TATTTTGAAG
    GACAAACTCA CATACGACTC TTACTCTCCT GATACCTTCC TGGAGATGGA TTTGAAACAA
    AAATCACAAG ATTCTCTGCC ACAAAAAAAT ATAATAAGGC TGAGTGCGGA GCCAAGGTAC
    CTTGTGGTCT ATAATCCTTT AGAACAAGAC CGAATCTCGT TGGTCTCAGT CTATGTGAGT
    TCCCCGACAG TGCAAGTGTT CTCTGCTTCA GGAAAACCTG TGGAAGTTCA AGTCAGCGCA
    GTTTGGGATA CAGCAAATAC TATTTCAGAA ACAGCCTATG AGATCTCTTT TCGAGCACAT
    ATACCGCCAT TGGGACTGAA AGTGTATAAG ATTTTGGAAT CAGCAAGTTC AAATTCACAT
    TTAGCTGATT ATGTCTTGTA TAAGAATAAA GTAGAAGATA GCGGAATTTT CACCATAAAG
    AATATGATAA ATACTGAAGA AGGTATAACA CTAGAGAACT CCTTTGTTTT ACTTCGGTTT
    GATCAAACTG GACTTATGAA GCAAATGATG ACTAAAGAAG ATGGTAAACA CCATGAAGTA
    AATGTGCAAT TTTCATGGTA TGGAACCACA ATTAAAAGAG ACAAAAGTGG TGCCTACCTC
    TTCTTACCTG ATGGTAATGC CAAGCCTTAT GTTTACACAA CACCGCCCTT TGTCAGAGTG
    ACACATGGAA GGATTTATTC GGAAGTGACT TGCTTTTTTG ACCATGTTAC TCATAGAGTC
    CGACTATACC ACATACAGGG AATAGAAGGA CAGTCTGTGG AAGTTTCCAA TATTGTGGAC
    ATCCGAAAAG TATATAACCG TGAGATTGCA ATGAAAATTT CTTCTGATAT AAAAAGCCAA
    AATAGATTTT ATACTGACCT AAATGGGTAC CAGATTCAAC CTAGAATGAC ACTGAGCAAA
    TTGCCTCTTC AAGCAAATGT CTATCCCATG ACCACAATGG CCTATATCCA GGATGCCAAA
    CATCGTTTGA CACTGCTCTC TGCTCAGTCA TTAGGGGTTT CGAGTTTGAA TAGTGGTCAG
    ATTGAAGTTA TCATGGATCG AAGACTCATG CAAGATGATA ATCGTGGCCT TGAGCAAGGT
    ATCCAGGATA ACAAGATTAC AGCTAATCTA TTTCGAATAC TACTAGAAAA AAGAAGTGCT
    GTTAATACGG AAGAAGAAAA GAAGTCGGTC AGTTATCCTT CTCTCCTTAG CCACATAACT
    TCTTCTCTCA TGAATCATCC AGTCATTCCA ATGGCAAATA AGTTCTCCTC ACCTACCCTT
    GAGCTGCAAG GTGAATTCTC TCCATTACAG TCATCTTTGC CTTGTGACAT TCATCTGGTT
    AATTTGAGAA CAATACAGTC AAAGGTGGGC AATGGGCACT CCAATGAGGC AGCCTTGATC
    CTCCACAGAA AAGGGTTTGA TTGTCGGTTC TCTAGCAAAG GCACAGGGCT GTTTTGTTCT
    ACTACTCAGG GAAAGATATT GGTACAGAAA CTTTTAAACA AGTTTATTGT CGAAAGTCTC
    ACACCTTCAT CACTATCCTT GATGCATTCA CCTCCCGGCA CTCAGAATAT AAGTGAGATC
    AACTTGAGTC CAATGGAAAT CAGCACATTC CGAATCCAGT TGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864961.1
    CDS121..3408
    Translation

    Target ORF information:

    RefSeq Version XM_017009472.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mannosidase alpha class 2A member 1 (MAN2A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017009472.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGCAAG AAAAAATAGA CCATTTGGAG CGTTTGCTAG CTGAGAATAA TGAGATCATC 
    TCAAATATTA GAGACTCAGT CATCAATTTG AGTGAGTCTG TGGAGGATGG TCCGAAAAGT
    TCACAAAGCA ATTTCAGCCA AGGTGCTGGC TCACATCTTC TGCCCTCACA ATTATCCCTC
    TCAGTTGACA CTGCAGACTG TCTGTTTGCT TCACAAAGTG GAAGTCACAA TTCAGATGTG
    CAGATGTTGG ATGTTTACAG TCTAATTTCT TTTGACAATC CAGATGGTGG AGTTTGGAAG
    CAAGGATTTG ACATTACTTA TGAATCTAAT GAATGGGACA CTGAACCCCT TCAAGTCTTT
    GTGGTGCCTC ATTCCCATAA CGACCCAGGT TGGTTGAAGA CTTTCAATGA CTACTTTAGA
    GACAAGACTC AGTATATTTT TAATAACATG GTCCTAAAGC TGAAAGAAGA CTCACGGAGG
    AAGTTTATTT GGTCTGAGAT CTCTTACCTT TCAAAGTGGT GGGATATTAT AGATATTCAG
    AAGAAGGATG CTGTTAAAAG TTTAATAGAA AATGGTCAGC TTGAAATTGT GACAGGTGGC
    TGGGTTATGC CTGATGAAGC TACTCCACAT TATTTTGCCT TAATTGATCA ACTAATTGAA
    GGACATCAGT GGCTGGAAAA TAATATAGGA GTGAAACCTC GGTCCGGCTG GGCTATTGAT
    CCCTTTGGAC ACTCACCAAC AATGGCTTAT CTTCTAAACC GTGCTGGACT TTCTCACATG
    CTTATCCAGA GAGTTCATTA TGCAGTTAAA AAACACTTTG CACTGCATAA AACATTGGAG
    TTTTTTTGGA GACAGAATTG GGATCTGGGA TCTGTCACAG ATATTTTATG CCACATGATG
    CCCTTCTACA GCTATGACAT CCCTCACACT TGTGGACCTG ATCCTAAAAT ATGCTGCCAG
    TTTGATTTTA AACGTCTTCC TGGAGGCAGA TTTGGTTGTC CCTGGGGAGT CCCCCCAGAA
    ACAATACATC CTGGAAATGT CCAAAGCAGG GCTCGGATGC TACTAGATCA GTACCGAAAG
    AAGTCAAAGC TTTTTCGTAC CAAAGTTCTC CTGGCTCCAC TAGGAGATGA TTTCCGCTAC
    TGTGAATACA CGGAATGGGA TTTACAGTTT AAGAATTATC AGCAGCTTTT TGATTATATG
    AATTCTCAGT CCAAGTTTAA AGTTAAGATA CAGTTTGGAA CTTTATCAGA TTTTTTTGAT
    GCGCTGGATA AAGCAGATGA AACTCAGAGA GACAAGGGCC AATCGATGTT CCCTGTTTTA
    AGTGGAGATT TTTTCACTTA TGCCGATCGA GATGATCATT ACTGGAGTGG CTATTTTACA
    TCCAGACCCT TTTACAAACG AATGGACAGA ATCATGGAAT CTCATTTAAG GGCTGCTGAA
    ATTCTTTACT ATTTCGCCCT GAGACAAGCT CACAAATACA AGATAAATAA ATTTCTCTCA
    TCATCACTTT ACACGGCACT GACAGAAGCC AGAAGGAATT TGGGACTGTT TCAACATCAT
    GATGCTATCA CAGGAACTGC AAAAGACTGG GTGGTTGTGG ATTATGGTAC CAGACTTTTT
    CATTCGTTAA TGGTTTTGGA GAAGATAATT GGAAATTCTG CATTTCTTCT TATTTTGAAG
    GACAAACTCA CATACGACTC TTACTCTCCT GATACCTTCC TGGAGATGGA TTTGAAACAA
    AAATCACAAG ATTCTCTGCC ACAAAAAAAT ATAATAAGGC TGAGTGCGGA GCCAAGGTAC
    CTTGTGGTCT ATAATCCTTT AGAACAAGAC CGAATCTCGT TGGTCTCAGT CTATGTGAGT
    TCCCCGACAG TGCAAGTGTT CTCTGCTTCA GGAAAACCTG TGGAAGTTCA AGTCAGCGCA
    GTTTGGGATA CAGCAAATAC TATTTCAGAA ACAGCCTATG AGATCTCTTT TCGAGCACAT
    ATACCGCCAT TGGGACTGAA AGTGTATAAG ATTTTGGAAT CAGCAAGTTC AAATTCACAT
    TTAGCTGATT ATGTCTTGTA TAAGAATAAA GTAGAAGATA GCGGAATTTT CACCATAAAG
    AATATGATAA ATACTGAAGA AGGTATAACA CTAGAGAACT CCTTTGTTTT ACTTCGGTTT
    GATCAAACTG GACTTATGAA GCAAATGATG ACTAAAGAAG ATGGTAAACA CCATGAAGTA
    AATGTGCAAT TTTCATGGTA TGGAACCACA ATTAAAAGAG ACAAAAGTGG TGCCTACCTC
    TTCTTACCTG ATGGTAATGC CAAGCCTTAT GTTTACACAA CACCGCCCTT TGTCAGAGTG
    ACACATGGAA GGATTTATTC GGAAGTGACT TGCTTTTTTG ACCATGTTAC TCATAGAGTC
    CGACTATACC ACATACAGGG AATAGAAGGA CAGTCTGTGG AAGTTTCCAA TATTGTGGAC
    ATCCGAAAAG TATATAACCG TGAGATTGCA ATGAAAATTT CTTCTGATAT AAAAAGCCAA
    AATAGATTTT ATACTGACCT AAATGGGTAC CAGATTCAAC CTAGAATGAC ACTGAGCAAA
    TTGCCTCTTC AAGCAAATGT CTATCCCATG ACCACAATGG CCTATATCCA GGATGCCAAA
    CATCGTTTGA CACTGCTCTC TGCTCAGTCA TTAGGGGTTT CGAGTTTGAA TAGTGGTCAG
    ATTGAAGTTA TCATGGATCG AAGACTCATG CAAGATGATA ATCGTGGCCT TGAGCAAGGT
    ATCCAGGATA ACAAGATTAC AGCTAATCTA TTTCGAATAC TACTAGAAAA AAGAAGTGCT
    GTTAATACGG AAGAAGAAAA GAAGTCGGTC AGTTATCCTT CTCTCCTTAG CCACATAACT
    TCTTCTCTCA TGAATCATCC AGTCATTCCA ATGGCAAATA AGTTCTCCTC ACCTACCCTT
    GAGCTGCAAG GTGAATTCTC TCCATTACAG TCATCTTTGC CTTGTGACAT TCATCTGGTT
    AATTTGAGAA CAATACAGTC AAAGGTGGGC AATGGGCACT CCAATGAGGC AGCCTTGATC
    CTCCACAGAA AAGGGTTTGA TTGTCGGTTC TCTAGCAAAG GCACAGGGCT GTTTTGTTCT
    ACTACTCAGG GAAAGATATT GGTACAGAAA CTTTTAAACA AGTTTATTGT CGAAAGTCTC
    ACACCTTCAT CACTATCCTT GATGCATTCA CCTCCCGGCA CTCAGAATAT AAGTGAGATC
    AACTTGAGTC CAATGGAAAT CAGCACATTC CGAATCCAGT TGAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.