LIPA cDNA ORF clone, Homo sapiens(human)

The following LIPA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIPA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23443 NM_000235.4
Latest version!
Homo sapiens lipase A, lysosomal acid type (LIPA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu23443 NM_001127605.3
Latest version!
Homo sapiens lipase A, lysosomal acid type (LIPA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu23877 NM_001288979.1
Latest version!
Homo sapiens lipase A, lysosomal acid type (LIPA), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu23443 XM_024448023.1
Latest version!
Homo sapiens lipase A, lysosomal acid type (LIPA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
View Old Accession Versions >>
OHu23443 NM_001127605.2 Homo sapiens lipase A, lysosomal acid type (LIPA), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu23443 NM_000235.3 Homo sapiens lipase A, lysosomal acid type (LIPA), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu23443
    Clone ID Related Accession (Same CDS sequence) XM_024448023.1 , NM_001127605.3 , NM_000235.3 , NM_001127605.2 , NM_000235.4
    Accession Version NM_001127605.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product lysosomal acid lipase/cholesteryl ester hydrolase isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY150944.1 and BC012287.1. On May 31, 2019 this sequence version replaced NM_001127605.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 2 encode the same isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.127557.1, SRR1803611.102356.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGC GGTTCTTGGG GTTGGTGGTC TGTTTGGTTC TCTGGACCCT GCATTCTGAG 
    GGGTCTGGAG GGAAACTGAC AGCTGTGGAT CCTGAAACAA ACATGAATGT GAGTGAAATT
    ATCTCTTACT GGGGATTCCC TAGTGAGGAA TACCTAGTTG AGACAGAAGA TGGATATATT
    CTGTGCCTTA ACCGAATTCC TCATGGGAGG AAGAACCATT CTGACAAAGG TCCCAAACCA
    GTTGTCTTCC TGCAACATGG CTTGCTGGCA GATTCTAGTA ACTGGGTCAC AAACCTTGCC
    AACAGCAGCC TGGGCTTCAT TCTTGCTGAT GCTGGTTTTG ACGTGTGGAT GGGCAACAGC
    AGAGGAAATA CCTGGTCTCG GAAACATAAG ACACTCTCAG TTTCTCAGGA TGAATTCTGG
    GCTTTCAGTT ATGATGAGAT GGCAAAATAT GACCTACCAG CTTCCATTAA CTTCATTCTG
    AATAAAACTG GCCAAGAACA AGTGTATTAT GTGGGTCATT CTCAAGGCAC CACTATAGGT
    TTTATAGCAT TTTCACAGAT CCCTGAGCTG GCTAAAAGGA TTAAAATGTT TTTTGCCCTG
    GGTCCTGTGG CTTCCGTCGC CTTCTGTACT AGCCCTATGG CCAAATTAGG ACGATTACCA
    GATCATCTCA TTAAGGACTT ATTTGGAGAC AAAGAATTTC TTCCCCAGAG TGCGTTTTTG
    AAGTGGCTGG GTACCCACGT TTGCACTCAT GTCATACTGA AGGAGCTCTG TGGAAATCTC
    TGTTTTCTTC TGTGTGGATT TAATGAGAGA AATTTAAATA TGTCTAGAGT GGATGTATAT
    ACAACACATT CTCCTGCTGG AACTTCTGTG CAAAACATGT TACACTGGAG CCAGGCTGTT
    AAATTCCAAA AGTTTCAAGC CTTTGACTGG GGAAGCAGTG CCAAGAATTA TTTTCATTAC
    AACCAGAGTT ATCCTCCCAC ATACAATGTG AAGGACATGC TTGTGCCGAC TGCAGTCTGG
    AGCGGGGGTC ACGACTGGCT TGCAGATGTC TACGACGTCA ATATCTTACT GACTCAGATC
    ACCAACTTGG TGTTCCATGA GAGCATTCCG GAATGGGAGC ATCTTGACTT CATTTGGGGC
    CTGGATGCCC CTTGGAGGCT TTATAATAAA ATTATTAATC TAATGAGGAA ATATCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121077.1
    CDS71..1270
    Translation

    Target ORF information:

    RefSeq Version NM_001127605.3
    Organism Homo sapiens(human)
    Definition Homo sapiens lipase A, lysosomal acid type (LIPA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127605.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGC GGTTCTTGGG GTTGGTGGTC TGTTTGGTTC TCTGGACCCT GCATTCTGAG 
    GGGTCTGGAG GGAAACTGAC AGCTGTGGAT CCTGAAACAA ACATGAATGT GAGTGAAATT
    ATCTCTTACT GGGGATTCCC TAGTGAGGAA TACCTAGTTG AGACAGAAGA TGGATATATT
    CTGTGCCTTA ACCGAATTCC TCATGGGAGG AAGAACCATT CTGACAAAGG TCCCAAACCA
    GTTGTCTTCC TGCAACATGG CTTGCTGGCA GATTCTAGTA ACTGGGTCAC AAACCTTGCC
    AACAGCAGCC TGGGCTTCAT TCTTGCTGAT GCTGGTTTTG ACGTGTGGAT GGGCAACAGC
    AGAGGAAATA CCTGGTCTCG GAAACATAAG ACACTCTCAG TTTCTCAGGA TGAATTCTGG
    GCTTTCAGTT ATGATGAGAT GGCAAAATAT GACCTACCAG CTTCCATTAA CTTCATTCTG
    AATAAAACTG GCCAAGAACA AGTGTATTAT GTGGGTCATT CTCAAGGCAC CACTATAGGT
    TTTATAGCAT TTTCACAGAT CCCTGAGCTG GCTAAAAGGA TTAAAATGTT TTTTGCCCTG
    GGTCCTGTGG CTTCCGTCGC CTTCTGTACT AGCCCTATGG CCAAATTAGG ACGATTACCA
    GATCATCTCA TTAAGGACTT ATTTGGAGAC AAAGAATTTC TTCCCCAGAG TGCGTTTTTG
    AAGTGGCTGG GTACCCACGT TTGCACTCAT GTCATACTGA AGGAGCTCTG TGGAAATCTC
    TGTTTTCTTC TGTGTGGATT TAATGAGAGA AATTTAAATA TGTCTAGAGT GGATGTATAT
    ACAACACATT CTCCTGCTGG AACTTCTGTG CAAAACATGT TACACTGGAG CCAGGCTGTT
    AAATTCCAAA AGTTTCAAGC CTTTGACTGG GGAAGCAGTG CCAAGAATTA TTTTCATTAC
    AACCAGAGTT ATCCTCCCAC ATACAATGTG AAGGACATGC TTGTGCCGAC TGCAGTCTGG
    AGCGGGGGTC ACGACTGGCT TGCAGATGTC TACGACGTCA ATATCTTACT GACTCAGATC
    ACCAACTTGG TGTTCCATGA GAGCATTCCG GAATGGGAGC ATCTTGACTT CATTTGGGGC
    CTGGATGCCC CTTGGAGGCT TTATAATAAA ATTATTAATC TAATGAGGAA ATATCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23443
    Clone ID Related Accession (Same CDS sequence) XM_024448023.1 , NM_001127605.3 , NM_000235.3 , NM_001127605.2 , NM_000235.4
    Accession Version NM_000235.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-31
    Organism Homo sapiens(human)
    Product lysosomal acid lipase/cholesteryl ester hydrolase isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA394611.1, BC012287.1 and CA435432.1. This sequence is a reference standard in the RefSeqGene project. On Jan 9, 2014 this sequence version replaced NM_000235.2. Transcript Variant: This variant (2) lacks an internal segment in the 5' UTR, as compared to variant 1. Variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.204582.1, SRR1803613.214291.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGC GGTTCTTGGG GTTGGTGGTC TGTTTGGTTC TCTGGACCCT GCATTCTGAG 
    GGGTCTGGAG GGAAACTGAC AGCTGTGGAT CCTGAAACAA ACATGAATGT GAGTGAAATT
    ATCTCTTACT GGGGATTCCC TAGTGAGGAA TACCTAGTTG AGACAGAAGA TGGATATATT
    CTGTGCCTTA ACCGAATTCC TCATGGGAGG AAGAACCATT CTGACAAAGG TCCCAAACCA
    GTTGTCTTCC TGCAACATGG CTTGCTGGCA GATTCTAGTA ACTGGGTCAC AAACCTTGCC
    AACAGCAGCC TGGGCTTCAT TCTTGCTGAT GCTGGTTTTG ACGTGTGGAT GGGCAACAGC
    AGAGGAAATA CCTGGTCTCG GAAACATAAG ACACTCTCAG TTTCTCAGGA TGAATTCTGG
    GCTTTCAGTT ATGATGAGAT GGCAAAATAT GACCTACCAG CTTCCATTAA CTTCATTCTG
    AATAAAACTG GCCAAGAACA AGTGTATTAT GTGGGTCATT CTCAAGGCAC CACTATAGGT
    TTTATAGCAT TTTCACAGAT CCCTGAGCTG GCTAAAAGGA TTAAAATGTT TTTTGCCCTG
    GGTCCTGTGG CTTCCGTCGC CTTCTGTACT AGCCCTATGG CCAAATTAGG ACGATTACCA
    GATCATCTCA TTAAGGACTT ATTTGGAGAC AAAGAATTTC TTCCCCAGAG TGCGTTTTTG
    AAGTGGCTGG GTACCCACGT TTGCACTCAT GTCATACTGA AGGAGCTCTG TGGAAATCTC
    TGTTTTCTTC TGTGTGGATT TAATGAGAGA AATTTAAATA TGTCTAGAGT GGATGTATAT
    ACAACACATT CTCCTGCTGG AACTTCTGTG CAAAACATGT TACACTGGAG CCAGGCTGTT
    AAATTCCAAA AGTTTCAAGC CTTTGACTGG GGAAGCAGTG CCAAGAATTA TTTTCATTAC
    AACCAGAGTT ATCCTCCCAC ATACAATGTG AAGGACATGC TTGTGCCGAC TGCAGTCTGG
    AGCGGGGGTC ACGACTGGCT TGCAGATGTC TACGACGTCA ATATCTTACT GACTCAGATC
    ACCAACTTGG TGTTCCATGA GAGCATTCCG GAATGGGAGC ATCTTGACTT CATTTGGGGC
    CTGGATGCCC CTTGGAGGCT TTATAATAAA ATTATTAATC TAATGAGGAA ATATCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000226.2
    CDS305..1504
    Translation

    Target ORF information:

    RefSeq Version NM_000235.3
    Organism Homo sapiens(human)
    Definition Homo sapiens lipase A, lysosomal acid type (LIPA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000235.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGC GGTTCTTGGG GTTGGTGGTC TGTTTGGTTC TCTGGACCCT GCATTCTGAG 
    GGGTCTGGAG GGAAACTGAC AGCTGTGGAT CCTGAAACAA ACATGAATGT GAGTGAAATT
    ATCTCTTACT GGGGATTCCC TAGTGAGGAA TACCTAGTTG AGACAGAAGA TGGATATATT
    CTGTGCCTTA ACCGAATTCC TCATGGGAGG AAGAACCATT CTGACAAAGG TCCCAAACCA
    GTTGTCTTCC TGCAACATGG CTTGCTGGCA GATTCTAGTA ACTGGGTCAC AAACCTTGCC
    AACAGCAGCC TGGGCTTCAT TCTTGCTGAT GCTGGTTTTG ACGTGTGGAT GGGCAACAGC
    AGAGGAAATA CCTGGTCTCG GAAACATAAG ACACTCTCAG TTTCTCAGGA TGAATTCTGG
    GCTTTCAGTT ATGATGAGAT GGCAAAATAT GACCTACCAG CTTCCATTAA CTTCATTCTG
    AATAAAACTG GCCAAGAACA AGTGTATTAT GTGGGTCATT CTCAAGGCAC CACTATAGGT
    TTTATAGCAT TTTCACAGAT CCCTGAGCTG GCTAAAAGGA TTAAAATGTT TTTTGCCCTG
    GGTCCTGTGG CTTCCGTCGC CTTCTGTACT AGCCCTATGG CCAAATTAGG ACGATTACCA
    GATCATCTCA TTAAGGACTT ATTTGGAGAC AAAGAATTTC TTCCCCAGAG TGCGTTTTTG
    AAGTGGCTGG GTACCCACGT TTGCACTCAT GTCATACTGA AGGAGCTCTG TGGAAATCTC
    TGTTTTCTTC TGTGTGGATT TAATGAGAGA AATTTAAATA TGTCTAGAGT GGATGTATAT
    ACAACACATT CTCCTGCTGG AACTTCTGTG CAAAACATGT TACACTGGAG CCAGGCTGTT
    AAATTCCAAA AGTTTCAAGC CTTTGACTGG GGAAGCAGTG CCAAGAATTA TTTTCATTAC
    AACCAGAGTT ATCCTCCCAC ATACAATGTG AAGGACATGC TTGTGCCGAC TGCAGTCTGG
    AGCGGGGGTC ACGACTGGCT TGCAGATGTC TACGACGTCA ATATCTTACT GACTCAGATC
    ACCAACTTGG TGTTCCATGA GAGCATTCCG GAATGGGAGC ATCTTGACTT CATTTGGGGC
    CTGGATGCCC CTTGGAGGCT TTATAATAAA ATTATTAATC TAATGAGGAA ATATCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23443
    Clone ID Related Accession (Same CDS sequence) XM_024448023.1 , NM_001127605.3 , NM_000235.3 , NM_001127605.2 , NM_000235.4
    Accession Version NM_001127605.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-25
    Organism Homo sapiens(human)
    Product lysosomal acid lipase/cholesteryl ester hydrolase isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY150944.1, BC012287.1 and CA435432.1. On Jan 9, 2014 this sequence version replaced NM_001127605.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 2 encode the same isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.113695.1, SRR1803613.59986.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGC GGTTCTTGGG GTTGGTGGTC TGTTTGGTTC TCTGGACCCT GCATTCTGAG 
    GGGTCTGGAG GGAAACTGAC AGCTGTGGAT CCTGAAACAA ACATGAATGT GAGTGAAATT
    ATCTCTTACT GGGGATTCCC TAGTGAGGAA TACCTAGTTG AGACAGAAGA TGGATATATT
    CTGTGCCTTA ACCGAATTCC TCATGGGAGG AAGAACCATT CTGACAAAGG TCCCAAACCA
    GTTGTCTTCC TGCAACATGG CTTGCTGGCA GATTCTAGTA ACTGGGTCAC AAACCTTGCC
    AACAGCAGCC TGGGCTTCAT TCTTGCTGAT GCTGGTTTTG ACGTGTGGAT GGGCAACAGC
    AGAGGAAATA CCTGGTCTCG GAAACATAAG ACACTCTCAG TTTCTCAGGA TGAATTCTGG
    GCTTTCAGTT ATGATGAGAT GGCAAAATAT GACCTACCAG CTTCCATTAA CTTCATTCTG
    AATAAAACTG GCCAAGAACA AGTGTATTAT GTGGGTCATT CTCAAGGCAC CACTATAGGT
    TTTATAGCAT TTTCACAGAT CCCTGAGCTG GCTAAAAGGA TTAAAATGTT TTTTGCCCTG
    GGTCCTGTGG CTTCCGTCGC CTTCTGTACT AGCCCTATGG CCAAATTAGG ACGATTACCA
    GATCATCTCA TTAAGGACTT ATTTGGAGAC AAAGAATTTC TTCCCCAGAG TGCGTTTTTG
    AAGTGGCTGG GTACCCACGT TTGCACTCAT GTCATACTGA AGGAGCTCTG TGGAAATCTC
    TGTTTTCTTC TGTGTGGATT TAATGAGAGA AATTTAAATA TGTCTAGAGT GGATGTATAT
    ACAACACATT CTCCTGCTGG AACTTCTGTG CAAAACATGT TACACTGGAG CCAGGCTGTT
    AAATTCCAAA AGTTTCAAGC CTTTGACTGG GGAAGCAGTG CCAAGAATTA TTTTCATTAC
    AACCAGAGTT ATCCTCCCAC ATACAATGTG AAGGACATGC TTGTGCCGAC TGCAGTCTGG
    AGCGGGGGTC ACGACTGGCT TGCAGATGTC TACGACGTCA ATATCTTACT GACTCAGATC
    ACCAACTTGG TGTTCCATGA GAGCATTCCG GAATGGGAGC ATCTTGACTT CATTTGGGGC
    CTGGATGCCC CTTGGAGGCT TTATAATAAA ATTATTAATC TAATGAGGAA ATATCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121077.1
    CDS204..1403
    Translation

    Target ORF information:

    RefSeq Version NM_001127605.2
    Organism Homo sapiens(human)
    Definition Homo sapiens lipase A, lysosomal acid type (LIPA), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127605.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGC GGTTCTTGGG GTTGGTGGTC TGTTTGGTTC TCTGGACCCT GCATTCTGAG 
    GGGTCTGGAG GGAAACTGAC AGCTGTGGAT CCTGAAACAA ACATGAATGT GAGTGAAATT
    ATCTCTTACT GGGGATTCCC TAGTGAGGAA TACCTAGTTG AGACAGAAGA TGGATATATT
    CTGTGCCTTA ACCGAATTCC TCATGGGAGG AAGAACCATT CTGACAAAGG TCCCAAACCA
    GTTGTCTTCC TGCAACATGG CTTGCTGGCA GATTCTAGTA ACTGGGTCAC AAACCTTGCC
    AACAGCAGCC TGGGCTTCAT TCTTGCTGAT GCTGGTTTTG ACGTGTGGAT GGGCAACAGC
    AGAGGAAATA CCTGGTCTCG GAAACATAAG ACACTCTCAG TTTCTCAGGA TGAATTCTGG
    GCTTTCAGTT ATGATGAGAT GGCAAAATAT GACCTACCAG CTTCCATTAA CTTCATTCTG
    AATAAAACTG GCCAAGAACA AGTGTATTAT GTGGGTCATT CTCAAGGCAC CACTATAGGT
    TTTATAGCAT TTTCACAGAT CCCTGAGCTG GCTAAAAGGA TTAAAATGTT TTTTGCCCTG
    GGTCCTGTGG CTTCCGTCGC CTTCTGTACT AGCCCTATGG CCAAATTAGG ACGATTACCA
    GATCATCTCA TTAAGGACTT ATTTGGAGAC AAAGAATTTC TTCCCCAGAG TGCGTTTTTG
    AAGTGGCTGG GTACCCACGT TTGCACTCAT GTCATACTGA AGGAGCTCTG TGGAAATCTC
    TGTTTTCTTC TGTGTGGATT TAATGAGAGA AATTTAAATA TGTCTAGAGT GGATGTATAT
    ACAACACATT CTCCTGCTGG AACTTCTGTG CAAAACATGT TACACTGGAG CCAGGCTGTT
    AAATTCCAAA AGTTTCAAGC CTTTGACTGG GGAAGCAGTG CCAAGAATTA TTTTCATTAC
    AACCAGAGTT ATCCTCCCAC ATACAATGTG AAGGACATGC TTGTGCCGAC TGCAGTCTGG
    AGCGGGGGTC ACGACTGGCT TGCAGATGTC TACGACGTCA ATATCTTACT GACTCAGATC
    ACCAACTTGG TGTTCCATGA GAGCATTCCG GAATGGGAGC ATCTTGACTT CATTTGGGGC
    CTGGATGCCC CTTGGAGGCT TTATAATAAA ATTATTAATC TAATGAGGAA ATATCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23443
    Clone ID Related Accession (Same CDS sequence) XM_024448023.1 , NM_001127605.3 , NM_000235.3 , NM_001127605.2 , NM_000235.4
    Accession Version NM_000235.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product lysosomal acid lipase/cholesteryl ester hydrolase isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA394611.1 and BC012287.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000235.3. Transcript Variant: This variant (2) lacks an internal segment in the 5' UTR, as compared to variant 1. Variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012287.1, X76488.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000336233.10/ ENSP00000337354.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGC GGTTCTTGGG GTTGGTGGTC TGTTTGGTTC TCTGGACCCT GCATTCTGAG 
    GGGTCTGGAG GGAAACTGAC AGCTGTGGAT CCTGAAACAA ACATGAATGT GAGTGAAATT
    ATCTCTTACT GGGGATTCCC TAGTGAGGAA TACCTAGTTG AGACAGAAGA TGGATATATT
    CTGTGCCTTA ACCGAATTCC TCATGGGAGG AAGAACCATT CTGACAAAGG TCCCAAACCA
    GTTGTCTTCC TGCAACATGG CTTGCTGGCA GATTCTAGTA ACTGGGTCAC AAACCTTGCC
    AACAGCAGCC TGGGCTTCAT TCTTGCTGAT GCTGGTTTTG ACGTGTGGAT GGGCAACAGC
    AGAGGAAATA CCTGGTCTCG GAAACATAAG ACACTCTCAG TTTCTCAGGA TGAATTCTGG
    GCTTTCAGTT ATGATGAGAT GGCAAAATAT GACCTACCAG CTTCCATTAA CTTCATTCTG
    AATAAAACTG GCCAAGAACA AGTGTATTAT GTGGGTCATT CTCAAGGCAC CACTATAGGT
    TTTATAGCAT TTTCACAGAT CCCTGAGCTG GCTAAAAGGA TTAAAATGTT TTTTGCCCTG
    GGTCCTGTGG CTTCCGTCGC CTTCTGTACT AGCCCTATGG CCAAATTAGG ACGATTACCA
    GATCATCTCA TTAAGGACTT ATTTGGAGAC AAAGAATTTC TTCCCCAGAG TGCGTTTTTG
    AAGTGGCTGG GTACCCACGT TTGCACTCAT GTCATACTGA AGGAGCTCTG TGGAAATCTC
    TGTTTTCTTC TGTGTGGATT TAATGAGAGA AATTTAAATA TGTCTAGAGT GGATGTATAT
    ACAACACATT CTCCTGCTGG AACTTCTGTG CAAAACATGT TACACTGGAG CCAGGCTGTT
    AAATTCCAAA AGTTTCAAGC CTTTGACTGG GGAAGCAGTG CCAAGAATTA TTTTCATTAC
    AACCAGAGTT ATCCTCCCAC ATACAATGTG AAGGACATGC TTGTGCCGAC TGCAGTCTGG
    AGCGGGGGTC ACGACTGGCT TGCAGATGTC TACGACGTCA ATATCTTACT GACTCAGATC
    ACCAACTTGG TGTTCCATGA GAGCATTCCG GAATGGGAGC ATCTTGACTT CATTTGGGGC
    CTGGATGCCC CTTGGAGGCT TTATAATAAA ATTATTAATC TAATGAGGAA ATATCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000226.2
    CDS41..1240
    Translation

    Target ORF information:

    RefSeq Version NM_000235.4
    Organism Homo sapiens(human)
    Definition Homo sapiens lipase A, lysosomal acid type (LIPA), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000235.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGC GGTTCTTGGG GTTGGTGGTC TGTTTGGTTC TCTGGACCCT GCATTCTGAG 
    GGGTCTGGAG GGAAACTGAC AGCTGTGGAT CCTGAAACAA ACATGAATGT GAGTGAAATT
    ATCTCTTACT GGGGATTCCC TAGTGAGGAA TACCTAGTTG AGACAGAAGA TGGATATATT
    CTGTGCCTTA ACCGAATTCC TCATGGGAGG AAGAACCATT CTGACAAAGG TCCCAAACCA
    GTTGTCTTCC TGCAACATGG CTTGCTGGCA GATTCTAGTA ACTGGGTCAC AAACCTTGCC
    AACAGCAGCC TGGGCTTCAT TCTTGCTGAT GCTGGTTTTG ACGTGTGGAT GGGCAACAGC
    AGAGGAAATA CCTGGTCTCG GAAACATAAG ACACTCTCAG TTTCTCAGGA TGAATTCTGG
    GCTTTCAGTT ATGATGAGAT GGCAAAATAT GACCTACCAG CTTCCATTAA CTTCATTCTG
    AATAAAACTG GCCAAGAACA AGTGTATTAT GTGGGTCATT CTCAAGGCAC CACTATAGGT
    TTTATAGCAT TTTCACAGAT CCCTGAGCTG GCTAAAAGGA TTAAAATGTT TTTTGCCCTG
    GGTCCTGTGG CTTCCGTCGC CTTCTGTACT AGCCCTATGG CCAAATTAGG ACGATTACCA
    GATCATCTCA TTAAGGACTT ATTTGGAGAC AAAGAATTTC TTCCCCAGAG TGCGTTTTTG
    AAGTGGCTGG GTACCCACGT TTGCACTCAT GTCATACTGA AGGAGCTCTG TGGAAATCTC
    TGTTTTCTTC TGTGTGGATT TAATGAGAGA AATTTAAATA TGTCTAGAGT GGATGTATAT
    ACAACACATT CTCCTGCTGG AACTTCTGTG CAAAACATGT TACACTGGAG CCAGGCTGTT
    AAATTCCAAA AGTTTCAAGC CTTTGACTGG GGAAGCAGTG CCAAGAATTA TTTTCATTAC
    AACCAGAGTT ATCCTCCCAC ATACAATGTG AAGGACATGC TTGTGCCGAC TGCAGTCTGG
    AGCGGGGGTC ACGACTGGCT TGCAGATGTC TACGACGTCA ATATCTTACT GACTCAGATC
    ACCAACTTGG TGTTCCATGA GAGCATTCCG GAATGGGAGC ATCTTGACTT CATTTGGGGC
    CTGGATGCCC CTTGGAGGCT TTATAATAAA ATTATTAATC TAATGAGGAA ATATCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23877
    Clone ID Related Accession (Same CDS sequence) NM_001288979.1
    Accession Version NM_001288979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-13
    Organism Homo sapiens(human)
    Product lysosomal acid lipase/cholesteryl ester hydrolase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA394611.1, DA466221.1, BC012287.1 and CA435432.1. Transcript Variant: This variant (3) lacks two consecutive exons in the 5' region, which results in translation initiation at a downstream AUG, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.270046.1, SRR1803617.8809.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGGCAACA GCAGAGGAAA TACCTGGTCT CGGAAACATA AGACACTCTC AGTTTCTCAG 
    GATGAATTCT GGGCTTTCAG TTATGATGAG ATGGCAAAAT ATGACCTACC AGCTTCCATT
    AACTTCATTC TGAATAAAAC TGGCCAAGAA CAAGTGTATT ATGTGGGTCA TTCTCAAGGC
    ACCACTATAG GTTTTATAGC ATTTTCACAG ATCCCTGAGC TGGCTAAAAG GATTAAAATG
    TTTTTTGCCC TGGGTCCTGT GGCTTCCGTC GCCTTCTGTA CTAGCCCTAT GGCCAAATTA
    GGACGATTAC CAGATCATCT CATTAAGGAC TTATTTGGAG ACAAAGAATT TCTTCCCCAG
    AGTGCGTTTT TGAAGTGGCT GGGTACCCAC GTTTGCACTC ATGTCATACT GAAGGAGCTC
    TGTGGAAATC TCTGTTTTCT TCTGTGTGGA TTTAATGAGA GAAATTTAAA TATGTCTAGA
    GTGGATGTAT ATACAACACA TTCTCCTGCT GGAACTTCTG TGCAAAACAT GTTACACTGG
    AGCCAGGCTG TTAAATTCCA AAAGTTTCAA GCCTTTGACT GGGGAAGCAG TGCCAAGAAT
    TATTTTCATT ACAACCAGAG TTATCCTCCC ACATACAATG TGAAGGACAT GCTTGTGCCG
    ACTGCAGTCT GGAGCGGGGG TCACGACTGG CTTGCAGATG TCTACGACGT CAATATCTTA
    CTGACTCAGA TCACCAACTT GGTGTTCCAT GAGAGCATTC CGGAATGGGA GCATCTTGAC
    TTCATTTGGG GCCTGGATGC CCCTTGGAGG CTTTATAATA AAATTATTAA TCTAATGAGG
    AAATATCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001275908.1
    CDS423..1274
    Translation

    Target ORF information:

    RefSeq Version NM_001288979.1
    Organism Homo sapiens(human)
    Definition Homo sapiens lipase A, lysosomal acid type (LIPA), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001288979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGGCAACA GCAGAGGAAA TACCTGGTCT CGGAAACATA AGACACTCTC AGTTTCTCAG 
    GATGAATTCT GGGCTTTCAG TTATGATGAG ATGGCAAAAT ATGACCTACC AGCTTCCATT
    AACTTCATTC TGAATAAAAC TGGCCAAGAA CAAGTGTATT ATGTGGGTCA TTCTCAAGGC
    ACCACTATAG GTTTTATAGC ATTTTCACAG ATCCCTGAGC TGGCTAAAAG GATTAAAATG
    TTTTTTGCCC TGGGTCCTGT GGCTTCCGTC GCCTTCTGTA CTAGCCCTAT GGCCAAATTA
    GGACGATTAC CAGATCATCT CATTAAGGAC TTATTTGGAG ACAAAGAATT TCTTCCCCAG
    AGTGCGTTTT TGAAGTGGCT GGGTACCCAC GTTTGCACTC ATGTCATACT GAAGGAGCTC
    TGTGGAAATC TCTGTTTTCT TCTGTGTGGA TTTAATGAGA GAAATTTAAA TATGTCTAGA
    GTGGATGTAT ATACAACACA TTCTCCTGCT GGAACTTCTG TGCAAAACAT GTTACACTGG
    AGCCAGGCTG TTAAATTCCA AAAGTTTCAA GCCTTTGACT GGGGAAGCAG TGCCAAGAAT
    TATTTTCATT ACAACCAGAG TTATCCTCCC ACATACAATG TGAAGGACAT GCTTGTGCCG
    ACTGCAGTCT GGAGCGGGGG TCACGACTGG CTTGCAGATG TCTACGACGT CAATATCTTA
    CTGACTCAGA TCACCAACTT GGTGTTCCAT GAGAGCATTC CGGAATGGGA GCATCTTGAC
    TTCATTTGGG GCCTGGATGC CCCTTGGAGG CTTTATAATA AAATTATTAA TCTAATGAGG
    AAATATCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu23443
    Clone ID Related Accession (Same CDS sequence) XM_024448023.1 , NM_001127605.3 , NM_000235.3 , NM_001127605.2 , NM_000235.4
    Accession Version XM_024448023.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product lysosomal acid lipase/cholesteryl ester hydrolase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGC GGTTCTTGGG GTTGGTGGTC TGTTTGGTTC TCTGGACCCT GCATTCTGAG 
    GGGTCTGGAG GGAAACTGAC AGCTGTGGAT CCTGAAACAA ACATGAATGT GAGTGAAATT
    ATCTCTTACT GGGGATTCCC TAGTGAGGAA TACCTAGTTG AGACAGAAGA TGGATATATT
    CTGTGCCTTA ACCGAATTCC TCATGGGAGG AAGAACCATT CTGACAAAGG TCCCAAACCA
    GTTGTCTTCC TGCAACATGG CTTGCTGGCA GATTCTAGTA ACTGGGTCAC AAACCTTGCC
    AACAGCAGCC TGGGCTTCAT TCTTGCTGAT GCTGGTTTTG ACGTGTGGAT GGGCAACAGC
    AGAGGAAATA CCTGGTCTCG GAAACATAAG ACACTCTCAG TTTCTCAGGA TGAATTCTGG
    GCTTTCAGTT ATGATGAGAT GGCAAAATAT GACCTACCAG CTTCCATTAA CTTCATTCTG
    AATAAAACTG GCCAAGAACA AGTGTATTAT GTGGGTCATT CTCAAGGCAC CACTATAGGT
    TTTATAGCAT TTTCACAGAT CCCTGAGCTG GCTAAAAGGA TTAAAATGTT TTTTGCCCTG
    GGTCCTGTGG CTTCCGTCGC CTTCTGTACT AGCCCTATGG CCAAATTAGG ACGATTACCA
    GATCATCTCA TTAAGGACTT ATTTGGAGAC AAAGAATTTC TTCCCCAGAG TGCGTTTTTG
    AAGTGGCTGG GTACCCACGT TTGCACTCAT GTCATACTGA AGGAGCTCTG TGGAAATCTC
    TGTTTTCTTC TGTGTGGATT TAATGAGAGA AATTTAAATA TGTCTAGAGT GGATGTATAT
    ACAACACATT CTCCTGCTGG AACTTCTGTG CAAAACATGT TACACTGGAG CCAGGCTGTT
    AAATTCCAAA AGTTTCAAGC CTTTGACTGG GGAAGCAGTG CCAAGAATTA TTTTCATTAC
    AACCAGAGTT ATCCTCCCAC ATACAATGTG AAGGACATGC TTGTGCCGAC TGCAGTCTGG
    AGCGGGGGTC ACGACTGGCT TGCAGATGTC TACGACGTCA ATATCTTACT GACTCAGATC
    ACCAACTTGG TGTTCCATGA GAGCATTCCG GAATGGGAGC ATCTTGACTT CATTTGGGGC
    CTGGATGCCC CTTGGAGGCT TTATAATAAA ATTATTAATC TAATGAGGAA ATATCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024303791.1
    CDS731..1930
    Translation

    Target ORF information:

    RefSeq Version XM_024448023.1
    Organism Homo sapiens(human)
    Definition Homo sapiens lipase A, lysosomal acid type (LIPA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024448023.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAATGC GGTTCTTGGG GTTGGTGGTC TGTTTGGTTC TCTGGACCCT GCATTCTGAG 
    GGGTCTGGAG GGAAACTGAC AGCTGTGGAT CCTGAAACAA ACATGAATGT GAGTGAAATT
    ATCTCTTACT GGGGATTCCC TAGTGAGGAA TACCTAGTTG AGACAGAAGA TGGATATATT
    CTGTGCCTTA ACCGAATTCC TCATGGGAGG AAGAACCATT CTGACAAAGG TCCCAAACCA
    GTTGTCTTCC TGCAACATGG CTTGCTGGCA GATTCTAGTA ACTGGGTCAC AAACCTTGCC
    AACAGCAGCC TGGGCTTCAT TCTTGCTGAT GCTGGTTTTG ACGTGTGGAT GGGCAACAGC
    AGAGGAAATA CCTGGTCTCG GAAACATAAG ACACTCTCAG TTTCTCAGGA TGAATTCTGG
    GCTTTCAGTT ATGATGAGAT GGCAAAATAT GACCTACCAG CTTCCATTAA CTTCATTCTG
    AATAAAACTG GCCAAGAACA AGTGTATTAT GTGGGTCATT CTCAAGGCAC CACTATAGGT
    TTTATAGCAT TTTCACAGAT CCCTGAGCTG GCTAAAAGGA TTAAAATGTT TTTTGCCCTG
    GGTCCTGTGG CTTCCGTCGC CTTCTGTACT AGCCCTATGG CCAAATTAGG ACGATTACCA
    GATCATCTCA TTAAGGACTT ATTTGGAGAC AAAGAATTTC TTCCCCAGAG TGCGTTTTTG
    AAGTGGCTGG GTACCCACGT TTGCACTCAT GTCATACTGA AGGAGCTCTG TGGAAATCTC
    TGTTTTCTTC TGTGTGGATT TAATGAGAGA AATTTAAATA TGTCTAGAGT GGATGTATAT
    ACAACACATT CTCCTGCTGG AACTTCTGTG CAAAACATGT TACACTGGAG CCAGGCTGTT
    AAATTCCAAA AGTTTCAAGC CTTTGACTGG GGAAGCAGTG CCAAGAATTA TTTTCATTAC
    AACCAGAGTT ATCCTCCCAC ATACAATGTG AAGGACATGC TTGTGCCGAC TGCAGTCTGG
    AGCGGGGGTC ACGACTGGCT TGCAGATGTC TACGACGTCA ATATCTTACT GACTCAGATC
    ACCAACTTGG TGTTCCATGA GAGCATTCCG GAATGGGAGC ATCTTGACTT CATTTGGGGC
    CTGGATGCCC CTTGGAGGCT TTATAATAAA ATTATTAATC TAATGAGGAA ATATCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.