LIF cDNA ORF clone, Homo sapiens(Human)

The following LIF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu21284 NM_002309.5
Latest version!
Homo sapiens LIF interleukin 6 family cytokine (LIF), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu21284 XM_011530172.1
Latest version!
Homo sapiens leukemia inhibitory factor (LIF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu21284 XM_024452239.1
Latest version!
Homo sapiens LIF interleukin 6 family cytokine (LIF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu21284 XM_024452240.1
Latest version!
Homo sapiens LIF interleukin 6 family cytokine (LIF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu21284 XM_006724240.2
Latest version!
Homo sapiens leukemia inhibitory factor (LIF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
OHu12127 NM_001257135.2
Latest version!
Homo sapiens LIF interleukin 6 family cytokine (LIF), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
View Old Accession Versions >>
OHu12127 NM_001257135.1 Homo sapiens LIF interleukin 6 family cytokine (LIF), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu21284 NM_002309.4 Homo sapiens LIF, interleukin 6 family cytokine (LIF), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$99.50-$139.30
$199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu21284
    Clone ID Related Accession (Same CDS sequence) NM_002309.4 , XM_011530172.1 , XM_024452239.1 , XM_024452240.1 , NM_002309.5 , XM_006724240.2
    Accession Version NM_002309.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-17
    Organism Homo sapiens(human)
    Product leukemia inhibitory factor isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN409458.1, AC004264.1 and AI796373.1. This sequence is a reference standard in the RefSeqGene project. On Mar 15, 2012 this sequence version replaced NM_002309.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CN409458.1, X13967.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGTTGG TTCTGCACTG GAAACATGGG 
    GCGGGGAGCC CCCTCCCCAT CACCCCTGTC AACGCCACCT GTGCCATACG CCACCCATGT
    CACAACAACC TCATGAACCA GATCAGGAGC CAACTGGCAC AGCTCAATGG CAGTGCCAAT
    GCCCTCTTTA TTCTCTATTA CACAGCCCAG GGGGAGCCGT TCCCCAACAA CCTGGACAAG
    CTATGTGGCC CCAACGTGAC GGACTTCCCG CCCTTCCACG CCAACGGCAC GGAGAAGGCC
    AAGCTGGTGG AGCTGTACCG CATAGTCGTG TACCTTGGCA CCTCCCTGGG CAACATCACC
    CGGGACCAGA AGATCCTCAA CCCCAGTGCC CTCAGCCTCC ACAGCAAGCT CAACGCCACC
    GCCGACATCC TGCGAGGCCT CCTTAGCAAC GTGCTGTGCC GCCTGTGCAG CAAGTACCAC
    GTGGGCCATG TGGACGTGAC CTACGGCCCT GACACCTCGG GTAAGGATGT CTTCCAGAAG
    AAGAAGCTGG GCTGTCAACT CCTGGGGAAG TATAAGCAGA TCATCGCCGT GTTGGCCCAG
    GCCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002300.1
    CDS157..765
    Translation

    Target ORF information:

    RefSeq Version NM_002309.4
    Organism Homo sapiens(human)
    Definition Homo sapiens LIF, interleukin 6 family cytokine (LIF), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002309.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGTTGG TTCTGCACTG GAAACATGGG 
    GCGGGGAGCC CCCTCCCCAT CACCCCTGTC AACGCCACCT GTGCCATACG CCACCCATGT
    CACAACAACC TCATGAACCA GATCAGGAGC CAACTGGCAC AGCTCAATGG CAGTGCCAAT
    GCCCTCTTTA TTCTCTATTA CACAGCCCAG GGGGAGCCGT TCCCCAACAA CCTGGACAAG
    CTATGTGGCC CCAACGTGAC GGACTTCCCG CCCTTCCACG CCAACGGCAC GGAGAAGGCC
    AAGCTGGTGG AGCTGTACCG CATAGTCGTG TACCTTGGCA CCTCCCTGGG CAACATCACC
    CGGGACCAGA AGATCCTCAA CCCCAGTGCC CTCAGCCTCC ACAGCAAGCT CAACGCCACC
    GCCGACATCC TGCGAGGCCT CCTTAGCAAC GTGCTGTGCC GCCTGTGCAG CAAGTACCAC
    GTGGGCCATG TGGACGTGAC CTACGGCCCT GACACCTCGG GTAAGGATGT CTTCCAGAAG
    AAGAAGCTGG GCTGTCAACT CCTGGGGAAG TATAAGCAGA TCATCGCCGT GTTGGCCCAG
    GCCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21284
    Clone ID Related Accession (Same CDS sequence) NM_002309.4 , XM_011530172.1 , XM_024452239.1 , XM_024452240.1 , NM_002309.5 , XM_006724240.2
    Accession Version NM_002309.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product leukemia inhibitory factor isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN409458.1, AC004264.1 and AI796373.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_002309.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP287018.1, X13967.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000249075.4/ ENSP00000249075.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGTTGG TTCTGCACTG GAAACATGGG 
    GCGGGGAGCC CCCTCCCCAT CACCCCTGTC AACGCCACCT GTGCCATACG CCACCCATGT
    CACAACAACC TCATGAACCA GATCAGGAGC CAACTGGCAC AGCTCAATGG CAGTGCCAAT
    GCCCTCTTTA TTCTCTATTA CACAGCCCAG GGGGAGCCGT TCCCCAACAA CCTGGACAAG
    CTATGTGGCC CCAACGTGAC GGACTTCCCG CCCTTCCACG CCAACGGCAC GGAGAAGGCC
    AAGCTGGTGG AGCTGTACCG CATAGTCGTG TACCTTGGCA CCTCCCTGGG CAACATCACC
    CGGGACCAGA AGATCCTCAA CCCCAGTGCC CTCAGCCTCC ACAGCAAGCT CAACGCCACC
    GCCGACATCC TGCGAGGCCT CCTTAGCAAC GTGCTGTGCC GCCTGTGCAG CAAGTACCAC
    GTGGGCCATG TGGACGTGAC CTACGGCCCT GACACCTCGG GTAAGGATGT CTTCCAGAAG
    AAGAAGCTGG GCTGTCAACT CCTGGGGAAG TATAAGCAGA TCATCGCCGT GTTGGCCCAG
    GCCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002300.1
    CDS65..673
    Translation

    Target ORF information:

    RefSeq Version NM_002309.5
    Organism Homo sapiens(human)
    Definition Homo sapiens LIF interleukin 6 family cytokine (LIF), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002309.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGTTGG TTCTGCACTG GAAACATGGG 
    GCGGGGAGCC CCCTCCCCAT CACCCCTGTC AACGCCACCT GTGCCATACG CCACCCATGT
    CACAACAACC TCATGAACCA GATCAGGAGC CAACTGGCAC AGCTCAATGG CAGTGCCAAT
    GCCCTCTTTA TTCTCTATTA CACAGCCCAG GGGGAGCCGT TCCCCAACAA CCTGGACAAG
    CTATGTGGCC CCAACGTGAC GGACTTCCCG CCCTTCCACG CCAACGGCAC GGAGAAGGCC
    AAGCTGGTGG AGCTGTACCG CATAGTCGTG TACCTTGGCA CCTCCCTGGG CAACATCACC
    CGGGACCAGA AGATCCTCAA CCCCAGTGCC CTCAGCCTCC ACAGCAAGCT CAACGCCACC
    GCCGACATCC TGCGAGGCCT CCTTAGCAAC GTGCTGTGCC GCCTGTGCAG CAAGTACCAC
    GTGGGCCATG TGGACGTGAC CTACGGCCCT GACACCTCGG GTAAGGATGT CTTCCAGAAG
    AAGAAGCTGG GCTGTCAACT CCTGGGGAAG TATAAGCAGA TCATCGCCGT GTTGGCCCAG
    GCCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21284
    Clone ID Related Accession (Same CDS sequence) NM_002309.4 , XM_011530172.1 , XM_024452239.1 , XM_024452240.1 , NM_002309.5 , XM_006724240.2
    Accession Version XM_011530172.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product leukemia inhibitory factor isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGTTGG TTCTGCACTG GAAACATGGG 
    GCGGGGAGCC CCCTCCCCAT CACCCCTGTC AACGCCACCT GTGCCATACG CCACCCATGT
    CACAACAACC TCATGAACCA GATCAGGAGC CAACTGGCAC AGCTCAATGG CAGTGCCAAT
    GCCCTCTTTA TTCTCTATTA CACAGCCCAG GGGGAGCCGT TCCCCAACAA CCTGGACAAG
    CTATGTGGCC CCAACGTGAC GGACTTCCCG CCCTTCCACG CCAACGGCAC GGAGAAGGCC
    AAGCTGGTGG AGCTGTACCG CATAGTCGTG TACCTTGGCA CCTCCCTGGG CAACATCACC
    CGGGACCAGA AGATCCTCAA CCCCAGTGCC CTCAGCCTCC ACAGCAAGCT CAACGCCACC
    GCCGACATCC TGCGAGGCCT CCTTAGCAAC GTGCTGTGCC GCCTGTGCAG CAAGTACCAC
    GTGGGCCATG TGGACGTGAC CTACGGCCCT GACACCTCGG GTAAGGATGT CTTCCAGAAG
    AAGAAGCTGG GCTGTCAACT CCTGGGGAAG TATAAGCAGA TCATCGCCGT GTTGGCCCAG
    GCCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528474.1
    Translation

    Target ORF information:

    RefSeq Version XM_011530172.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens leukemia inhibitory factor (LIF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530172.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGTTGG TTCTGCACTG GAAACATGGG 
    GCGGGGAGCC CCCTCCCCAT CACCCCTGTC AACGCCACCT GTGCCATACG CCACCCATGT
    CACAACAACC TCATGAACCA GATCAGGAGC CAACTGGCAC AGCTCAATGG CAGTGCCAAT
    GCCCTCTTTA TTCTCTATTA CACAGCCCAG GGGGAGCCGT TCCCCAACAA CCTGGACAAG
    CTATGTGGCC CCAACGTGAC GGACTTCCCG CCCTTCCACG CCAACGGCAC GGAGAAGGCC
    AAGCTGGTGG AGCTGTACCG CATAGTCGTG TACCTTGGCA CCTCCCTGGG CAACATCACC
    CGGGACCAGA AGATCCTCAA CCCCAGTGCC CTCAGCCTCC ACAGCAAGCT CAACGCCACC
    GCCGACATCC TGCGAGGCCT CCTTAGCAAC GTGCTGTGCC GCCTGTGCAG CAAGTACCAC
    GTGGGCCATG TGGACGTGAC CTACGGCCCT GACACCTCGG GTAAGGATGT CTTCCAGAAG
    AAGAAGCTGG GCTGTCAACT CCTGGGGAAG TATAAGCAGA TCATCGCCGT GTTGGCCCAG
    GCCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21284
    Clone ID Related Accession (Same CDS sequence) NM_002309.4 , XM_011530172.1 , XM_024452239.1 , XM_024452240.1 , NM_002309.5 , XM_006724240.2
    Accession Version XM_024452239.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product leukemia inhibitory factor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGTTGG TTCTGCACTG GAAACATGGG 
    GCGGGGAGCC CCCTCCCCAT CACCCCTGTC AACGCCACCT GTGCCATACG CCACCCATGT
    CACAACAACC TCATGAACCA GATCAGGAGC CAACTGGCAC AGCTCAATGG CAGTGCCAAT
    GCCCTCTTTA TTCTCTATTA CACAGCCCAG GGGGAGCCGT TCCCCAACAA CCTGGACAAG
    CTATGTGGCC CCAACGTGAC GGACTTCCCG CCCTTCCACG CCAACGGCAC GGAGAAGGCC
    AAGCTGGTGG AGCTGTACCG CATAGTCGTG TACCTTGGCA CCTCCCTGGG CAACATCACC
    CGGGACCAGA AGATCCTCAA CCCCAGTGCC CTCAGCCTCC ACAGCAAGCT CAACGCCACC
    GCCGACATCC TGCGAGGCCT CCTTAGCAAC GTGCTGTGCC GCCTGTGCAG CAAGTACCAC
    GTGGGCCATG TGGACGTGAC CTACGGCCCT GACACCTCGG GTAAGGATGT CTTCCAGAAG
    AAGAAGCTGG GCTGTCAACT CCTGGGGAAG TATAAGCAGA TCATCGCCGT GTTGGCCCAG
    GCCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308007.1
    CDS265..873
    Translation

    Target ORF information:

    RefSeq Version XM_024452239.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIF interleukin 6 family cytokine (LIF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452239.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGTTGG TTCTGCACTG GAAACATGGG 
    GCGGGGAGCC CCCTCCCCAT CACCCCTGTC AACGCCACCT GTGCCATACG CCACCCATGT
    CACAACAACC TCATGAACCA GATCAGGAGC CAACTGGCAC AGCTCAATGG CAGTGCCAAT
    GCCCTCTTTA TTCTCTATTA CACAGCCCAG GGGGAGCCGT TCCCCAACAA CCTGGACAAG
    CTATGTGGCC CCAACGTGAC GGACTTCCCG CCCTTCCACG CCAACGGCAC GGAGAAGGCC
    AAGCTGGTGG AGCTGTACCG CATAGTCGTG TACCTTGGCA CCTCCCTGGG CAACATCACC
    CGGGACCAGA AGATCCTCAA CCCCAGTGCC CTCAGCCTCC ACAGCAAGCT CAACGCCACC
    GCCGACATCC TGCGAGGCCT CCTTAGCAAC GTGCTGTGCC GCCTGTGCAG CAAGTACCAC
    GTGGGCCATG TGGACGTGAC CTACGGCCCT GACACCTCGG GTAAGGATGT CTTCCAGAAG
    AAGAAGCTGG GCTGTCAACT CCTGGGGAAG TATAAGCAGA TCATCGCCGT GTTGGCCCAG
    GCCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21284
    Clone ID Related Accession (Same CDS sequence) NM_002309.4 , XM_011530172.1 , XM_024452239.1 , XM_024452240.1 , NM_002309.5 , XM_006724240.2
    Accession Version XM_024452240.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product leukemia inhibitory factor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGTTGG TTCTGCACTG GAAACATGGG 
    GCGGGGAGCC CCCTCCCCAT CACCCCTGTC AACGCCACCT GTGCCATACG CCACCCATGT
    CACAACAACC TCATGAACCA GATCAGGAGC CAACTGGCAC AGCTCAATGG CAGTGCCAAT
    GCCCTCTTTA TTCTCTATTA CACAGCCCAG GGGGAGCCGT TCCCCAACAA CCTGGACAAG
    CTATGTGGCC CCAACGTGAC GGACTTCCCG CCCTTCCACG CCAACGGCAC GGAGAAGGCC
    AAGCTGGTGG AGCTGTACCG CATAGTCGTG TACCTTGGCA CCTCCCTGGG CAACATCACC
    CGGGACCAGA AGATCCTCAA CCCCAGTGCC CTCAGCCTCC ACAGCAAGCT CAACGCCACC
    GCCGACATCC TGCGAGGCCT CCTTAGCAAC GTGCTGTGCC GCCTGTGCAG CAAGTACCAC
    GTGGGCCATG TGGACGTGAC CTACGGCCCT GACACCTCGG GTAAGGATGT CTTCCAGAAG
    AAGAAGCTGG GCTGTCAACT CCTGGGGAAG TATAAGCAGA TCATCGCCGT GTTGGCCCAG
    GCCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308008.1
    CDS285..893
    Translation

    Target ORF information:

    RefSeq Version XM_024452240.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIF interleukin 6 family cytokine (LIF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452240.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGTTGG TTCTGCACTG GAAACATGGG 
    GCGGGGAGCC CCCTCCCCAT CACCCCTGTC AACGCCACCT GTGCCATACG CCACCCATGT
    CACAACAACC TCATGAACCA GATCAGGAGC CAACTGGCAC AGCTCAATGG CAGTGCCAAT
    GCCCTCTTTA TTCTCTATTA CACAGCCCAG GGGGAGCCGT TCCCCAACAA CCTGGACAAG
    CTATGTGGCC CCAACGTGAC GGACTTCCCG CCCTTCCACG CCAACGGCAC GGAGAAGGCC
    AAGCTGGTGG AGCTGTACCG CATAGTCGTG TACCTTGGCA CCTCCCTGGG CAACATCACC
    CGGGACCAGA AGATCCTCAA CCCCAGTGCC CTCAGCCTCC ACAGCAAGCT CAACGCCACC
    GCCGACATCC TGCGAGGCCT CCTTAGCAAC GTGCTGTGCC GCCTGTGCAG CAAGTACCAC
    GTGGGCCATG TGGACGTGAC CTACGGCCCT GACACCTCGG GTAAGGATGT CTTCCAGAAG
    AAGAAGCTGG GCTGTCAACT CCTGGGGAAG TATAAGCAGA TCATCGCCGT GTTGGCCCAG
    GCCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21284
    Clone ID Related Accession (Same CDS sequence) NM_002309.4 , XM_011530172.1 , XM_024452239.1 , XM_024452240.1 , NM_002309.5 , XM_006724240.2
    Accession Version XM_006724240.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product leukemia inhibitory factor isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGTTGG TTCTGCACTG GAAACATGGG 
    GCGGGGAGCC CCCTCCCCAT CACCCCTGTC AACGCCACCT GTGCCATACG CCACCCATGT
    CACAACAACC TCATGAACCA GATCAGGAGC CAACTGGCAC AGCTCAATGG CAGTGCCAAT
    GCCCTCTTTA TTCTCTATTA CACAGCCCAG GGGGAGCCGT TCCCCAACAA CCTGGACAAG
    CTATGTGGCC CCAACGTGAC GGACTTCCCG CCCTTCCACG CCAACGGCAC GGAGAAGGCC
    AAGCTGGTGG AGCTGTACCG CATAGTCGTG TACCTTGGCA CCTCCCTGGG CAACATCACC
    CGGGACCAGA AGATCCTCAA CCCCAGTGCC CTCAGCCTCC ACAGCAAGCT CAACGCCACC
    GCCGACATCC TGCGAGGCCT CCTTAGCAAC GTGCTGTGCC GCCTGTGCAG CAAGTACCAC
    GTGGGCCATG TGGACGTGAC CTACGGCCCT GACACCTCGG GTAAGGATGT CTTCCAGAAG
    AAGAAGCTGG GCTGTCAACT CCTGGGGAAG TATAAGCAGA TCATCGCCGT GTTGGCCCAG
    GCCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006724303.1
    Translation

    Target ORF information:

    RefSeq Version XM_006724240.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens leukemia inhibitory factor (LIF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006724240.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAGGTCT TGGCGGCAGG AGTTGTGCCC CTGCTGTTGG TTCTGCACTG GAAACATGGG 
    GCGGGGAGCC CCCTCCCCAT CACCCCTGTC AACGCCACCT GTGCCATACG CCACCCATGT
    CACAACAACC TCATGAACCA GATCAGGAGC CAACTGGCAC AGCTCAATGG CAGTGCCAAT
    GCCCTCTTTA TTCTCTATTA CACAGCCCAG GGGGAGCCGT TCCCCAACAA CCTGGACAAG
    CTATGTGGCC CCAACGTGAC GGACTTCCCG CCCTTCCACG CCAACGGCAC GGAGAAGGCC
    AAGCTGGTGG AGCTGTACCG CATAGTCGTG TACCTTGGCA CCTCCCTGGG CAACATCACC
    CGGGACCAGA AGATCCTCAA CCCCAGTGCC CTCAGCCTCC ACAGCAAGCT CAACGCCACC
    GCCGACATCC TGCGAGGCCT CCTTAGCAAC GTGCTGTGCC GCCTGTGCAG CAAGTACCAC
    GTGGGCCATG TGGACGTGAC CTACGGCCCT GACACCTCGG GTAAGGATGT CTTCCAGAAG
    AAGAAGCTGG GCTGTCAACT CCTGGGGAAG TATAAGCAGA TCATCGCCGT GTTGGCCCAG
    GCCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12127
    Clone ID Related Accession (Same CDS sequence) NM_001257135.2 , NM_001257135.1
    Accession Version NM_001257135.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-02
    Organism Homo sapiens(human)
    Product leukemia inhibitory factor isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN409458.1, BC143305.1, AC004264.1 and AI796373.1. On May 31, 2019 this sequence version replaced NM_001257135.1. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CN409468.1, BC143305.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    ATGAAGGTCT TGGCGGCAGT ACACAGCCCA GGGGGAGCCG TTCCCCAACA ACCTGGACAA 
    GCTATGTGGC CCCAACGTGA CGGACTTCCC GCCCTTCCAC GCCAACGGCA CGGAGAAGGC
    CAAGCTGGTG GAGCTGTACC GCATAGTCGT GTACCTTGGC ACCTCCCTGG GCAACATCAC
    CCGGGACCAG AAGATCCTCA ACCCCAGTGC CCTCAGCCTC CACAGCAAGC TCAACGCCAC
    CGCCGACATC CTGCGAGGCC TCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001244064.1
    CDS65..331
    Translation

    Target ORF information:

    RefSeq Version NM_001257135.2
    Organism Homo sapiens(human)
    Definition Homo sapiens LIF interleukin 6 family cytokine (LIF), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001257135.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    ATGAAGGTCT TGGCGGCAGT ACACAGCCCA GGGGGAGCCG TTCCCCAACA ACCTGGACAA 
    GCTATGTGGC CCCAACGTGA CGGACTTCCC GCCCTTCCAC GCCAACGGCA CGGAGAAGGC
    CAAGCTGGTG GAGCTGTACC GCATAGTCGT GTACCTTGGC ACCTCCCTGG GCAACATCAC
    CCGGGACCAG AAGATCCTCA ACCCCAGTGC CCTCAGCCTC CACAGCAAGC TCAACGCCAC
    CGCCGACATC CTGCGAGGCC TCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12127
    Clone ID Related Accession (Same CDS sequence) NM_001257135.2 , NM_001257135.1
    Accession Version NM_001257135.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product leukemia inhibitory factor isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN409458.1, BC143305.1, AC004264.1 and AI796373.1. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CN409468.1, BC143305.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    ATGAAGGTCT TGGCGGCAGT ACACAGCCCA GGGGGAGCCG TTCCCCAACA ACCTGGACAA 
    GCTATGTGGC CCCAACGTGA CGGACTTCCC GCCCTTCCAC GCCAACGGCA CGGAGAAGGC
    CAAGCTGGTG GAGCTGTACC GCATAGTCGT GTACCTTGGC ACCTCCCTGG GCAACATCAC
    CCGGGACCAG AAGATCCTCA ACCCCAGTGC CCTCAGCCTC CACAGCAAGC TCAACGCCAC
    CGCCGACATC CTGCGAGGCC TCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001244064.1
    CDS157..423
    Translation

    Target ORF information:

    RefSeq Version NM_001257135.1
    Organism Homo sapiens(human)
    Definition Homo sapiens LIF interleukin 6 family cytokine (LIF), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001257135.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    ATGAAGGTCT TGGCGGCAGT ACACAGCCCA GGGGGAGCCG TTCCCCAACA ACCTGGACAA 
    GCTATGTGGC CCCAACGTGA CGGACTTCCC GCCCTTCCAC GCCAACGGCA CGGAGAAGGC
    CAAGCTGGTG GAGCTGTACC GCATAGTCGT GTACCTTGGC ACCTCCCTGG GCAACATCAC
    CCGGGACCAG AAGATCCTCA ACCCCAGTGC CCTCAGCCTC CACAGCAAGC TCAACGCCAC
    CGCCGACATC CTGCGAGGCC TCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.