LFNG cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LFNG
Entrez Gene ID	3955
Full Name	LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms	SCDO3
General protein information	Preferred Names LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase Names beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p22.3
Summary	This gene is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This gene product is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009].
Disorder MIM:	602576
Disorder Html:	?Spondylocostal dysostosis 3, autosomal recessive, 609813 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001040168.1	NP_001035258.1	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform b precursor
NM_001040167.1	NP_001035257.1	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform a precursor
NM_002304.2	NP_002295.1	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform d
NM_001040168.2	NP_001035258.1	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform b precursor
NM_001040167.2	NP_001035257.1	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform a precursor
NM_001166355.1	NP_001159827.1	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform c

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LFNG	XP_527647.3
Bos taurus (cattle)	LFNG	NP_001039687.1
Xenopus tropicalis (tropical clawed frog)	lfng	NP_001017051.1
Homo sapiens (human)	LFNG	NP_001035257.1
Mus musculus (house mouse)	Lfng	NP_032520.1
	LOC100428736	XP_002803236.1
Rattus norvegicus (Norway rat)	Lfng	NP_596884.1
Gallus gallus (chicken)	LFNG	NP_990279.1
Canis lupus familiaris (dog)	LFNG	XP_547009.3
Danio rerio (zebrafish)	lfng	NP_571046.1

Gene Ontology
Bibliography

Related articles in PubMed

Lunatic Fringe and p53 Cooperatively Suppress Mesenchymal Stem-Like Breast Cancer.
Chung WC, Zhang S, Challagundla L, Zhou Y, Xu K
Neoplasia (New York, N.Y.)19(11)885-895(2017 Nov)

Comparative genomics reveals that loss of lunatic fringe (LFNG) promotes melanoma metastasis.
Del Castillo Velasco-Herrera M, van der Weyden L, Nsengimana J, Speak AO, Sj?berg MK, Bishop DT, J?nsson G, Newton-Bishop J, Adams DJ
Molecular oncology(2017 Nov)

Reconstitution of TGFBR2 in HCT116 colorectal cancer cells causes increased LFNG expression and enhanced N-acetyl-d-glucosamine incorporation into Notch1.
Lee J, Katzenmaier EM, Kopitz J, Gebert J
Cellular signalling28(8)1105-13(2016 08)

Lunatic Fringe is a potent tumor suppressor in Kras-initiated pancreatic cancer.
Zhang S, Chung WC, Xu K
Oncogene35(19)2485-95(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TGFBR2 signaling can affect Notch1 glycosylation via regulation of glycosyltransferase LFNG expression and provide a first mechanistic example for altered glycosylation in microsatellite instability colorectal tumor cells.
Title: Reconstitution of TGFBR2 in HCT116 colorectal cancer cells causes increased LFNG expression and enhanced N-acetyl-d-glucosamine incorporation into Notch1.

a potent tumor-suppressive function for Lfng
Title: Lunatic Fringe is a potent tumor suppressor in Kras-initiated pancreatic cancer.

LFNG expression correlates with expansion of cancer stem cell populations and NKX3.1 expression in human prostate cancer.
Title: Tumor-suppressive activity of Lunatic Fringe in prostate through differential modulation of Notch receptor activation.

Reduced LFNG expression facilitates JAG/NOTCH luminal progenitor signaling and cooperates with MET/CAVEOLIN basal-type signaling to promote basal-like breast cancer.
Title: Lunatic fringe deficiency cooperates with the Met/Caveolin gene amplicon to induce basal-like breast cancer.

Mutation of the LFNG gene causes spondylocostal dysostosis with a severe vertebral phenotype, reinforcing the hypothesis that proper regulation of the Notch signaling pathway is an absolute requirement for the correct patterning of the axial skeleton.
Title: Mutation of the LUNATIC FRINGE gene in humans causes spondylocostal dysostosis with a severe vertebral phenotype.

The following LFNG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LFNG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26170	NM_002304.2 Latest version!	Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
OHu26348	NM_001166355.1 Latest version!	Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu25867	NM_001040168.2 Latest version!	Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$209.30 $299.00
OHu25960	NM_001040167.2 Latest version!	Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu25867	NM_001040168.1	Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$209.30 $299.00
OHu25960	NM_001040167.1	Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LFNG ( NM_002304.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002295.1 Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu26170
Clone ID Related Accession (Same CDS sequence)	NM_002304.2
Accession Version	NM_002304.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC014851.1 and AK096284.1. On Oct 23, 2009 this sequence version replaced NM_002304.1. Transcript Variant: This variant (4) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at an alternate start codon, compared to variant 1. The encoded protein (isoform d) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014851.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155751 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACACCAG GTCGCTGCTG TCTTGCGGCT GACATTCAGG TAGAGACGTT CATCTTCACT 
GACGGGGAAG ATGAGGCCCT GGCCAGGCAC ACGGGCAACG TGGTCATCAC AAACTGCTCG 
GCCGCCCACA GCCGCCAGGC GCTGTCCTGC AAGATGGCCG TGGAGTATGA CCGCTTCATC 
GAGTCCGGCA GGAAGTGGTT CTGCCACGTG GACGATGACA ACTACGTCAA CCTGCGGGCC 
CTGCTGCGGC TGCTGGCCAG CTACCCGCAC ACGCGGGACG TCTACGTCGG CAAGCCCAGC 
CTGGACAGGC CCATCCAGGC CATGGAGCGG GTCAGCGAGA ACAAGGTGCG TCCTGTCCAC 
TTCTGGTTTG CCACGGGCGG CGCTGGCTTC TGCATCAGCC GTGGGCTGGC TCTGAAGATG 
AGCCCGTGGG CCAGCGGGGG TCACTTCATG AATACGGCTG AGCGGATCCG GCTGCCTGAT 
GACTGCACCA TCGGCTACAT CGTGGAGGCC CTGCTGGGTG TGCCCCTCAT CCGCAGCGGC 
CTCTTCCACT CCCACCTGGA GAACCTGCAG CAGGTGCCCA CCTCGGAGCT CCACGAGCAG 
GTGACGCTGA GCTACGGTAT GTTTGAAAAC AAGCGGAACG CCGTCCACGT GAAGGGGCCC 
TTCTCGGTGG AGGCCGACCC ATCCAGGTTC CGCTCCATCC ACTGCCACCT GTACCCGGAC 
ACACCCTGGT GTCCCCGCAC TGCCATCTTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002295.1
CDS	89..841
Translation	MTPGRCCLAADIQVETFIFTDGEDEALARHTGNVVITNCSAAHSRQALSCKMAVEYDRFIESGRKWFCHVDDDNYVNLRALLRLLASYPHTRDVYVGKPSLDRPIQAMERVSENKVRPVHFWFATGGAGFCISRGLALKMSPWASGGHFMNTAERIRLPDDCTIGYIVEALLGVPLIRSGLFHSHLENLQQVPTSELHEQVTLSYGMFENKRNAVHVKGPFSVEADPSRFRSIHCHLYPDTPWCPRTAIF

Target ORF information:

RefSeq Version	NM_002304.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002304.2

ORF Insert Sequence:

ATGACACCAG GTCGCTGCTG TCTTGCGGCT GACATTCAGG TAGAGACGTT CATCTTCACT 
GACGGGGAAG ATGAGGCCCT GGCCAGGCAC ACGGGCAACG TGGTCATCAC AAACTGCTCG 
GCCGCCCACA GCCGCCAGGC GCTGTCCTGC AAGATGGCCG TGGAGTATGA CCGCTTCATC 
GAGTCCGGCA GGAAGTGGTT CTGCCACGTG GACGATGACA ACTACGTCAA CCTGCGGGCC 
CTGCTGCGGC TGCTGGCCAG CTACCCGCAC ACGCGGGACG TCTACGTCGG CAAGCCCAGC 
CTGGACAGGC CCATCCAGGC CATGGAGCGG GTCAGCGAGA ACAAGGTGCG TCCTGTCCAC 
TTCTGGTTTG CCACGGGCGG CGCTGGCTTC TGCATCAGCC GTGGGCTGGC TCTGAAGATG 
AGCCCGTGGG CCAGCGGGGG TCACTTCATG AATACGGCTG AGCGGATCCG GCTGCCTGAT 
GACTGCACCA TCGGCTACAT CGTGGAGGCC CTGCTGGGTG TGCCCCTCAT CCGCAGCGGC 
CTCTTCCACT CCCACCTGGA GAACCTGCAG CAGGTGCCCA CCTCGGAGCT CCACGAGCAG 
GTGACGCTGA GCTACGGTAT GTTTGAAAAC AAGCGGAACG CCGTCCACGT GAAGGGGCCC 
TTCTCGGTGG AGGCCGACCC ATCCAGGTTC CGCTCCATCC ACTGCCACCT GTACCCGGAC 
ACACCCTGGT GTCCCCGCAC TGCCATCTTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LFNG ( NM_001166355.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159827.1 Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu26348
Clone ID Related Accession (Same CDS sequence)	NM_001166355.1
Accession Version	NM_001166355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Homo sapiens(human)
Product	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096284.1 and BC014851.1. Transcript Variant: This variant (3) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at an alternate start codon, compared to variant 1. The encoded protein (isoform c) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096284.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2152719, SAMN01820689 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGAAC AGACAGGAAG GCTCAGGCTG GACACGTATT GTATGAGTGC CAAGCAGATC 
TGGGCATGGA GCAAATGCTC AGGAAGGCTG TGGGATGAGC ACATGAAATG GATGGAAGGA 
TGGACGGACA GATGGACAGA TGGATGGATG GATGGATGGA TGGATGAGTG GAGCCCAACA 
CCAGCTCTCA GGTCCTACGG AGGTGGCCTC TCTCAGCAGA CGTTCATCTT CACTGACGGG 
GAAGATGAGG CCCTGGCCAG GCACACGGGC AACGTGGTCA TCACAAACTG CTCGGCCGCC 
CACAGCCGCC AGGCGCTGTC CTGCAAGATG GCCGTGGAGT ATGACCGCTT CATCGAGTCC 
GGCAGGAAGT GGTTCTGCCA CGTGGACGAT GACAACTACG TCAACCTGCG GGCCCTGCTG 
CGGCTGCTGG CCAGCTACCC GCACACGCGG GACGTCTACG TCGGCAAGCC CAGCCTGGAC 
AGGCCCATCC AGGCCATGGA GCGGGTCAGC GAGAACAAGG TGCGTCCTGT CCACTTCTGG 
TTTGCCACGG GCGGCGCTGG CTTCTGCATC AGCCGTGGGC TGGCTCTGAA GATGAGCCCG 
TGGGCCAGCG GGGGTCACTT CATGAATACG GCTGAGCGGA TCCGGCTGCC TGATGACTGC 
ACCATCGGCT ACATCGTGGA GGCCCTGCTG GGTGTGCCCC TCATCCGCAG CGGCCTCTTC 
CACTCCCACC TGGAGAACCT GCAGCAGGTG CCCACCTCGG AGCTCCACGA GCAGGTGACG 
CTGAGCTACG GTATGTTTGA AAACAAGCGG AACGCCGTCC ACGTGAAGGG GCCCTTCTCG 
GTGGAGGCCG ACCCATCCAG GTTCCGCTCC ATCCACTGCC ACCTGTACCC GGACACACCC 
TGGTGTCCCC GCACTGCCAT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159827.1
CDS	127..1053
Translation	MDEQTGRLRLDTYCMSAKQIWAWSKCSGRLWDEHMKWMEGWTDRWTDGWMDGWMDEWSPTPALRSYGGGLSQQTFIFTDGEDEALARHTGNVVITNCSAAHSRQALSCKMAVEYDRFIESGRKWFCHVDDDNYVNLRALLRLLASYPHTRDVYVGKPSLDRPIQAMERVSENKVRPVHFWFATGGAGFCISRGLALKMSPWASGGHFMNTAERIRLPDDCTIGYIVEALLGVPLIRSGLFHSHLENLQQVPTSELHEQVTLSYGMFENKRNAVHVKGPFSVEADPSRFRSIHCHLYPDTPWCPRTAIF

Target ORF information:

RefSeq Version	NM_001166355.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166355.1

ORF Insert Sequence:

ATGGATGAAC AGACAGGAAG GCTCAGGCTG GACACGTATT GTATGAGTGC CAAGCAGATC 
TGGGCATGGA GCAAATGCTC AGGAAGGCTG TGGGATGAGC ACATGAAATG GATGGAAGGA 
TGGACGGACA GATGGACAGA TGGATGGATG GATGGATGGA TGGATGAGTG GAGCCCAACA 
CCAGCTCTCA GGTCCTACGG AGGTGGCCTC TCTCAGCAGA CGTTCATCTT CACTGACGGG 
GAAGATGAGG CCCTGGCCAG GCACACGGGC AACGTGGTCA TCACAAACTG CTCGGCCGCC 
CACAGCCGCC AGGCGCTGTC CTGCAAGATG GCCGTGGAGT ATGACCGCTT CATCGAGTCC 
GGCAGGAAGT GGTTCTGCCA CGTGGACGAT GACAACTACG TCAACCTGCG GGCCCTGCTG 
CGGCTGCTGG CCAGCTACCC GCACACGCGG GACGTCTACG TCGGCAAGCC CAGCCTGGAC 
AGGCCCATCC AGGCCATGGA GCGGGTCAGC GAGAACAAGG TGCGTCCTGT CCACTTCTGG 
TTTGCCACGG GCGGCGCTGG CTTCTGCATC AGCCGTGGGC TGGCTCTGAA GATGAGCCCG 
TGGGCCAGCG GGGGTCACTT CATGAATACG GCTGAGCGGA TCCGGCTGCC TGATGACTGC 
ACCATCGGCT ACATCGTGGA GGCCCTGCTG GGTGTGCCCC TCATCCGCAG CGGCCTCTTC 
CACTCCCACC TGGAGAACCT GCAGCAGGTG CCCACCTCGG AGCTCCACGA GCAGGTGACG 
CTGAGCTACG GTATGTTTGA AAACAAGCGG AACGCCGTCC ACGTGAAGGG GCCCTTCTCG 
GTGGAGGCCG ACCCATCCAG GTTCCGCTCC ATCCACTGCC ACCTGTACCC GGACACACCC 
TGGTGTCCCC GCACTGCCAT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LFNG ( NM_001040168.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035258.1 Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25867
Clone ID Related Accession (Same CDS sequence)	NM_001040168.1 , NM_001040168.2
Accession Version	NM_001040168.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092488.3, U94354.1 and AF193612.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) includes an alternate 3' terminal exon compared to variant 1. The resulting protein (isoform b) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U94354.1, AF193612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCAAGC GCTGCGGCCG GCGCCTGCTG CTGGCGCTGG CGGGCGCGCT GCTCGCCTGC 
CTGCTGGTGC TCACCGCCGA CCCGCCGCCG CCTCCACTGC CCGCCGAGCG CGGCCGGCGC 
GCGCTGCGCA GCCTGGCGGG CCCCGCGGGG GCTGCCCCGG CGCCCGGGCT GGGGGCGGCG 
GCGGCGGCGC CCGGGGCGCT GGTCCGCGAC GTGCACAGTC TGTCCGAGTA CTTCAGCCTG 
CTCACCCGCG CGCGCAGAGA TGCGGGCCCG CCGCCCGGGG CTGCCCCCCG CCCCGCCGAC 
GGCCACCCGC GCCCCCTGGC CGAGCCGCTC GCGCCCCGAG ACGTCTTCAT CGCTGTCAAG 
ACCACCAAAA AGTTCCACCG CGCGCGCCTC GACCTGCTGC TGGAGACCTG GATCTCGCGC 
CACAAGGAGA TGACGTTCAT CTTCACTGAC GGGGAAGATG AGGCCCTGGC CAGGCACACG 
GGCAACGTGG TCATCACAAA CTGCTCGGCC GCCCACAGCC GCCAGGCGCT GTCCTGCAAG 
ATGGCCGTGG AGTATGACCG CTTCATCGAG TCCGGCAGGA AGTGGTTCTG CCACGTGGAC 
GATGACAACT ACGTCAACCT GCGGGCCCTG CTGCGGCTGC TGGCCAGCTA CCCGCACACG 
CGGGACGTCT ACGTCGGCAA GCCCAGCCTG GACAGGCCCA TCCAGGCCAT GGAGCGGGTC 
AGCGAGAACA AGGTGCGTCC TGTCCACTTC TGGTTTGCCA CGGGCGGCGC TGGCTTCTGC 
ATCAGCCGTG GGCTGGCTCT GAAGATGAGC CCGTGGGCCA GCGGGGGTCA CTTCATGAAT 
ACGGCTGAGC GGATCCGGCT GCCTGATGAC TGCACCATCG GCTACATCGT GGAGGCCCTG 
CTGGGTGTGC CCCTCATCCG CAGCGGCCTC TTCCACTCCC ACCTGGAGAA CCTGCAGCAG 
GTGCCCACCT CGGAGCTCCA CGAGCAGGTG ACGCTGAGCT ACGGTATGTT TGAAAACAAG 
CGGAACGCCG TCCACGTGAA GGGGCCCTTC TCGGTGGAGG CCGACCCATC CAGGTGGGGA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035258.1
CDS	18..1103
Translation	MLKRCGRRLLLALAGALLACLLVLTADPPPPPLPAERGRRALRSLAGPAGAAPAPGLGAAAAAPGALVRDVHSLSEYFSLLTRARRDAGPPPGAAPRPADGHPRPLAEPLAPRDVFIAVKTTKKFHRARLDLLLETWISRHKEMTFIFTDGEDEALARHTGNVVITNCSAAHSRQALSCKMAVEYDRFIESGRKWFCHVDDDNYVNLRALLRLLASYPHTRDVYVGKPSLDRPIQAMERVSENKVRPVHFWFATGGAGFCISRGLALKMSPWASGGHFMNTAERIRLPDDCTIGYIVEALLGVPLIRSGLFHSHLENLQQVPTSELHEQVTLSYGMFENKRNAVHVKGPFSVEADPSRWGN

Target ORF information:

RefSeq Version	NM_001040168.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040168.1

ORF Insert Sequence:

ATGCTCAAGC GCTGCGGCCG GCGCCTGCTG CTGGCGCTGG CGGGCGCGCT GCTCGCCTGC 
CTGCTGGTGC TCACCGCCGA CCCGCCGCCG CCTCCACTGC CCGCCGAGCG CGGCCGGCGC 
GCGCTGCGCA GCCTGGCGGG CCCCGCGGGG GCTGCCCCGG CGCCCGGGCT GGGGGCGGCG 
GCGGCGGCGC CCGGGGCGCT GGTCCGCGAC GTGCACAGTC TGTCCGAGTA CTTCAGCCTG 
CTCACCCGCG CGCGCAGAGA TGCGGGCCCG CCGCCCGGGG CTGCCCCCCG CCCCGCCGAC 
GGCCACCCGC GCCCCCTGGC CGAGCCGCTC GCGCCCCGAG ACGTCTTCAT CGCTGTCAAG 
ACCACCAAAA AGTTCCACCG CGCGCGCCTC GACCTGCTGC TGGAGACCTG GATCTCGCGC 
CACAAGGAGA TGACGTTCAT CTTCACTGAC GGGGAAGATG AGGCCCTGGC CAGGCACACG 
GGCAACGTGG TCATCACAAA CTGCTCGGCC GCCCACAGCC GCCAGGCGCT GTCCTGCAAG 
ATGGCCGTGG AGTATGACCG CTTCATCGAG TCCGGCAGGA AGTGGTTCTG CCACGTGGAC 
GATGACAACT ACGTCAACCT GCGGGCCCTG CTGCGGCTGC TGGCCAGCTA CCCGCACACG 
CGGGACGTCT ACGTCGGCAA GCCCAGCCTG GACAGGCCCA TCCAGGCCAT GGAGCGGGTC 
AGCGAGAACA AGGTGCGTCC TGTCCACTTC TGGTTTGCCA CGGGCGGCGC TGGCTTCTGC 
ATCAGCCGTG GGCTGGCTCT GAAGATGAGC CCGTGGGCCA GCGGGGGTCA CTTCATGAAT 
ACGGCTGAGC GGATCCGGCT GCCTGATGAC TGCACCATCG GCTACATCGT GGAGGCCCTG 
CTGGGTGTGC CCCTCATCCG CAGCGGCCTC TTCCACTCCC ACCTGGAGAA CCTGCAGCAG 
GTGCCCACCT CGGAGCTCCA CGAGCAGGTG ACGCTGAGCT ACGGTATGTT TGAAAACAAG 
CGGAACGCCG TCCACGTGAA GGGGCCCTTC TCGGTGGAGG CCGACCCATC CAGGTGGGGA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LFNG ( NM_001040168.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035258.1 Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25867
Clone ID Related Accession (Same CDS sequence)	NM_001040168.1 , NM_001040168.2
Accession Version	NM_001040168.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092488.3, U94354.1 and AF193612.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001040168.1. Transcript Variant: This variant (2) includes an alternate 3' terminal exon compared to variant 1. The resulting protein (isoform b) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U94354.1, AF193612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCAAGC GCTGCGGCCG GCGCCTGCTG CTGGCGCTGG CGGGCGCGCT GCTCGCCTGC 
CTGCTGGTGC TCACCGCCGA CCCGCCGCCG CCTCCACTGC CCGCCGAGCG CGGCCGGCGC 
GCGCTGCGCA GCCTGGCGGG CCCCGCGGGG GCTGCCCCGG CGCCCGGGCT GGGGGCGGCG 
GCGGCGGCGC CCGGGGCGCT GGTCCGCGAC GTGCACAGTC TGTCCGAGTA CTTCAGCCTG 
CTCACCCGCG CGCGCAGAGA TGCGGGCCCG CCGCCCGGGG CTGCCCCCCG CCCCGCCGAC 
GGCCACCCGC GCCCCCTGGC CGAGCCGCTC GCGCCCCGAG ACGTCTTCAT CGCTGTCAAG 
ACCACCAAAA AGTTCCACCG CGCGCGCCTC GACCTGCTGC TGGAGACCTG GATCTCGCGC 
CACAAGGAGA TGACGTTCAT CTTCACTGAC GGGGAAGATG AGGCCCTGGC CAGGCACACG 
GGCAACGTGG TCATCACAAA CTGCTCGGCC GCCCACAGCC GCCAGGCGCT GTCCTGCAAG 
ATGGCCGTGG AGTATGACCG CTTCATCGAG TCCGGCAGGA AGTGGTTCTG CCACGTGGAC 
GATGACAACT ACGTCAACCT GCGGGCCCTG CTGCGGCTGC TGGCCAGCTA CCCGCACACG 
CGGGACGTCT ACGTCGGCAA GCCCAGCCTG GACAGGCCCA TCCAGGCCAT GGAGCGGGTC 
AGCGAGAACA AGGTGCGTCC TGTCCACTTC TGGTTTGCCA CGGGCGGCGC TGGCTTCTGC 
ATCAGCCGTG GGCTGGCTCT GAAGATGAGC CCGTGGGCCA GCGGGGGTCA CTTCATGAAT 
ACGGCTGAGC GGATCCGGCT GCCTGATGAC TGCACCATCG GCTACATCGT GGAGGCCCTG 
CTGGGTGTGC CCCTCATCCG CAGCGGCCTC TTCCACTCCC ACCTGGAGAA CCTGCAGCAG 
GTGCCCACCT CGGAGCTCCA CGAGCAGGTG ACGCTGAGCT ACGGTATGTT TGAAAACAAG 
CGGAACGCCG TCCACGTGAA GGGGCCCTTC TCGGTGGAGG CCGACCCATC CAGGTGGGGA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035258.1
CDS	89..1174
Translation	MLKRCGRRLLLALAGALLACLLVLTADPPPPPLPAERGRRALRSLAGPAGAAPAPGLGAAAAAPGALVRDVHSLSEYFSLLTRARRDAGPPPGAAPRPADGHPRPLAEPLAPRDVFIAVKTTKKFHRARLDLLLETWISRHKEMTFIFTDGEDEALARHTGNVVITNCSAAHSRQALSCKMAVEYDRFIESGRKWFCHVDDDNYVNLRALLRLLASYPHTRDVYVGKPSLDRPIQAMERVSENKVRPVHFWFATGGAGFCISRGLALKMSPWASGGHFMNTAERIRLPDDCTIGYIVEALLGVPLIRSGLFHSHLENLQQVPTSELHEQVTLSYGMFENKRNAVHVKGPFSVEADPSRWGN

Target ORF information:

RefSeq Version	NM_001040168.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040168.2

ORF Insert Sequence:

ATGCTCAAGC GCTGCGGCCG GCGCCTGCTG CTGGCGCTGG CGGGCGCGCT GCTCGCCTGC 
CTGCTGGTGC TCACCGCCGA CCCGCCGCCG CCTCCACTGC CCGCCGAGCG CGGCCGGCGC 
GCGCTGCGCA GCCTGGCGGG CCCCGCGGGG GCTGCCCCGG CGCCCGGGCT GGGGGCGGCG 
GCGGCGGCGC CCGGGGCGCT GGTCCGCGAC GTGCACAGTC TGTCCGAGTA CTTCAGCCTG 
CTCACCCGCG CGCGCAGAGA TGCGGGCCCG CCGCCCGGGG CTGCCCCCCG CCCCGCCGAC 
GGCCACCCGC GCCCCCTGGC CGAGCCGCTC GCGCCCCGAG ACGTCTTCAT CGCTGTCAAG 
ACCACCAAAA AGTTCCACCG CGCGCGCCTC GACCTGCTGC TGGAGACCTG GATCTCGCGC 
CACAAGGAGA TGACGTTCAT CTTCACTGAC GGGGAAGATG AGGCCCTGGC CAGGCACACG 
GGCAACGTGG TCATCACAAA CTGCTCGGCC GCCCACAGCC GCCAGGCGCT GTCCTGCAAG 
ATGGCCGTGG AGTATGACCG CTTCATCGAG TCCGGCAGGA AGTGGTTCTG CCACGTGGAC 
GATGACAACT ACGTCAACCT GCGGGCCCTG CTGCGGCTGC TGGCCAGCTA CCCGCACACG 
CGGGACGTCT ACGTCGGCAA GCCCAGCCTG GACAGGCCCA TCCAGGCCAT GGAGCGGGTC 
AGCGAGAACA AGGTGCGTCC TGTCCACTTC TGGTTTGCCA CGGGCGGCGC TGGCTTCTGC 
ATCAGCCGTG GGCTGGCTCT GAAGATGAGC CCGTGGGCCA GCGGGGGTCA CTTCATGAAT 
ACGGCTGAGC GGATCCGGCT GCCTGATGAC TGCACCATCG GCTACATCGT GGAGGCCCTG 
CTGGGTGTGC CCCTCATCCG CAGCGGCCTC TTCCACTCCC ACCTGGAGAA CCTGCAGCAG 
GTGCCCACCT CGGAGCTCCA CGAGCAGGTG ACGCTGAGCT ACGGTATGTT TGAAAACAAG 
CGGAACGCCG TCCACGTGAA GGGGCCCTTC TCGGTGGAGG CCGACCCATC CAGGTGGGGA 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LFNG ( NM_001040167.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035257.1 Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 1, mRNA.

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CloneID	OHu25960
Clone ID Related Accession (Same CDS sequence)	NM_001040167.1 , NM_001040167.2
Accession Version	NM_001040167.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092488.3, U94354.1, BC014851.1 and BM854210.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCAAGC GCTGCGGCCG GCGCCTGCTG CTGGCGCTGG CGGGCGCGCT GCTCGCCTGC 
CTGCTGGTGC TCACCGCCGA CCCGCCGCCG CCTCCACTGC CCGCCGAGCG CGGCCGGCGC 
GCGCTGCGCA GCCTGGCGGG CCCCGCGGGG GCTGCCCCGG CGCCCGGGCT GGGGGCGGCG 
GCGGCGGCGC CCGGGGCGCT GGTCCGCGAC GTGCACAGTC TGTCCGAGTA CTTCAGCCTG 
CTCACCCGCG CGCGCAGAGA TGCGGGCCCG CCGCCCGGGG CTGCCCCCCG CCCCGCCGAC 
GGCCACCCGC GCCCCCTGGC CGAGCCGCTC GCGCCCCGAG ACGTCTTCAT CGCTGTCAAG 
ACCACCAAAA AGTTCCACCG CGCGCGCCTC GACCTGCTGC TGGAGACCTG GATCTCGCGC 
CACAAGGAGA TGACGTTCAT CTTCACTGAC GGGGAAGATG AGGCCCTGGC CAGGCACACG 
GGCAACGTGG TCATCACAAA CTGCTCGGCC GCCCACAGCC GCCAGGCGCT GTCCTGCAAG 
ATGGCCGTGG AGTATGACCG CTTCATCGAG TCCGGCAGGA AGTGGTTCTG CCACGTGGAC 
GATGACAACT ACGTCAACCT GCGGGCCCTG CTGCGGCTGC TGGCCAGCTA CCCGCACACG 
CGGGACGTCT ACGTCGGCAA GCCCAGCCTG GACAGGCCCA TCCAGGCCAT GGAGCGGGTC 
AGCGAGAACA AGGTGCGTCC TGTCCACTTC TGGTTTGCCA CGGGCGGCGC TGGCTTCTGC 
ATCAGCCGTG GGCTGGCTCT GAAGATGAGC CCGTGGGCCA GCGGGGGTCA CTTCATGAAT 
ACGGCTGAGC GGATCCGGCT GCCTGATGAC TGCACCATCG GCTACATCGT GGAGGCCCTG 
CTGGGTGTGC CCCTCATCCG CAGCGGCCTC TTCCACTCCC ACCTGGAGAA CCTGCAGCAG 
GTGCCCACCT CGGAGCTCCA CGAGCAGGTG ACGCTGAGCT ACGGTATGTT TGAAAACAAG 
CGGAACGCCG TCCACGTGAA GGGGCCCTTC TCGGTGGAGG CCGACCCATC CAGGTTCCGC 
TCCATCCACT GCCACCTGTA CCCGGACACA CCCTGGTGTC CCCGCACTGC CATCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035257.1
CDS	18..1157
Translation	MLKRCGRRLLLALAGALLACLLVLTADPPPPPLPAERGRRALRSLAGPAGAAPAPGLGAAAAAPGALVRDVHSLSEYFSLLTRARRDAGPPPGAAPRPADGHPRPLAEPLAPRDVFIAVKTTKKFHRARLDLLLETWISRHKEMTFIFTDGEDEALARHTGNVVITNCSAAHSRQALSCKMAVEYDRFIESGRKWFCHVDDDNYVNLRALLRLLASYPHTRDVYVGKPSLDRPIQAMERVSENKVRPVHFWFATGGAGFCISRGLALKMSPWASGGHFMNTAERIRLPDDCTIGYIVEALLGVPLIRSGLFHSHLENLQQVPTSELHEQVTLSYGMFENKRNAVHVKGPFSVEADPSRFRSIHCHLYPDTPWCPRTAIF

Target ORF information:

RefSeq Version	NM_001040167.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040167.1

ORF Insert Sequence:

ATGCTCAAGC GCTGCGGCCG GCGCCTGCTG CTGGCGCTGG CGGGCGCGCT GCTCGCCTGC 
CTGCTGGTGC TCACCGCCGA CCCGCCGCCG CCTCCACTGC CCGCCGAGCG CGGCCGGCGC 
GCGCTGCGCA GCCTGGCGGG CCCCGCGGGG GCTGCCCCGG CGCCCGGGCT GGGGGCGGCG 
GCGGCGGCGC CCGGGGCGCT GGTCCGCGAC GTGCACAGTC TGTCCGAGTA CTTCAGCCTG 
CTCACCCGCG CGCGCAGAGA TGCGGGCCCG CCGCCCGGGG CTGCCCCCCG CCCCGCCGAC 
GGCCACCCGC GCCCCCTGGC CGAGCCGCTC GCGCCCCGAG ACGTCTTCAT CGCTGTCAAG 
ACCACCAAAA AGTTCCACCG CGCGCGCCTC GACCTGCTGC TGGAGACCTG GATCTCGCGC 
CACAAGGAGA TGACGTTCAT CTTCACTGAC GGGGAAGATG AGGCCCTGGC CAGGCACACG 
GGCAACGTGG TCATCACAAA CTGCTCGGCC GCCCACAGCC GCCAGGCGCT GTCCTGCAAG 
ATGGCCGTGG AGTATGACCG CTTCATCGAG TCCGGCAGGA AGTGGTTCTG CCACGTGGAC 
GATGACAACT ACGTCAACCT GCGGGCCCTG CTGCGGCTGC TGGCCAGCTA CCCGCACACG 
CGGGACGTCT ACGTCGGCAA GCCCAGCCTG GACAGGCCCA TCCAGGCCAT GGAGCGGGTC 
AGCGAGAACA AGGTGCGTCC TGTCCACTTC TGGTTTGCCA CGGGCGGCGC TGGCTTCTGC 
ATCAGCCGTG GGCTGGCTCT GAAGATGAGC CCGTGGGCCA GCGGGGGTCA CTTCATGAAT 
ACGGCTGAGC GGATCCGGCT GCCTGATGAC TGCACCATCG GCTACATCGT GGAGGCCCTG 
CTGGGTGTGC CCCTCATCCG CAGCGGCCTC TTCCACTCCC ACCTGGAGAA CCTGCAGCAG 
GTGCCCACCT CGGAGCTCCA CGAGCAGGTG ACGCTGAGCT ACGGTATGTT TGAAAACAAG 
CGGAACGCCG TCCACGTGAA GGGGCCCTTC TCGGTGGAGG CCGACCCATC CAGGTTCCGC 
TCCATCCACT GCCACCTGTA CCCGGACACA CCCTGGTGTC CCCGCACTGC CATCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LFNG ( NM_001040167.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035257.1 Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25960
Clone ID Related Accession (Same CDS sequence)	NM_001040167.1 , NM_001040167.2
Accession Version	NM_001040167.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092488.3, U94354.1, BC014851.1 and BM854210.1. On Nov 23, 2018 this sequence version replaced NM_001040167.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000222725.10/ ENSP00000222725.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCAAGC GCTGCGGCCG GCGCCTGCTG CTGGCGCTGG CGGGCGCGCT GCTCGCCTGC 
CTGCTGGTGC TCACCGCCGA CCCGCCGCCG CCTCCACTGC CCGCCGAGCG CGGCCGGCGC 
GCGCTGCGCA GCCTGGCGGG CCCCGCGGGG GCTGCCCCGG CGCCCGGGCT GGGGGCGGCG 
GCGGCGGCGC CCGGGGCGCT GGTCCGCGAC GTGCACAGTC TGTCCGAGTA CTTCAGCCTG 
CTCACCCGCG CGCGCAGAGA TGCGGGCCCG CCGCCCGGGG CTGCCCCCCG CCCCGCCGAC 
GGCCACCCGC GCCCCCTGGC CGAGCCGCTC GCGCCCCGAG ACGTCTTCAT CGCTGTCAAG 
ACCACCAAAA AGTTCCACCG CGCGCGCCTC GACCTGCTGC TGGAGACCTG GATCTCGCGC 
CACAAGGAGA TGACGTTCAT CTTCACTGAC GGGGAAGATG AGGCCCTGGC CAGGCACACG 
GGCAACGTGG TCATCACAAA CTGCTCGGCC GCCCACAGCC GCCAGGCGCT GTCCTGCAAG 
ATGGCCGTGG AGTATGACCG CTTCATCGAG TCCGGCAGGA AGTGGTTCTG CCACGTGGAC 
GATGACAACT ACGTCAACCT GCGGGCCCTG CTGCGGCTGC TGGCCAGCTA CCCGCACACG 
CGGGACGTCT ACGTCGGCAA GCCCAGCCTG GACAGGCCCA TCCAGGCCAT GGAGCGGGTC 
AGCGAGAACA AGGTGCGTCC TGTCCACTTC TGGTTTGCCA CGGGCGGCGC TGGCTTCTGC 
ATCAGCCGTG GGCTGGCTCT GAAGATGAGC CCGTGGGCCA GCGGGGGTCA CTTCATGAAT 
ACGGCTGAGC GGATCCGGCT GCCTGATGAC TGCACCATCG GCTACATCGT GGAGGCCCTG 
CTGGGTGTGC CCCTCATCCG CAGCGGCCTC TTCCACTCCC ACCTGGAGAA CCTGCAGCAG 
GTGCCCACCT CGGAGCTCCA CGAGCAGGTG ACGCTGAGCT ACGGTATGTT TGAAAACAAG 
CGGAACGCCG TCCACGTGAA GGGGCCCTTC TCGGTGGAGG CCGACCCATC CAGGTTCCGC 
TCCATCCACT GCCACCTGTA CCCGGACACA CCCTGGTGTC CCCGCACTGC CATCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035257.1
CDS	89..1228
Translation	MLKRCGRRLLLALAGALLACLLVLTADPPPPPLPAERGRRALRSLAGPAGAAPAPGLGAAAAAPGALVRDVHSLSEYFSLLTRARRDAGPPPGAAPRPADGHPRPLAEPLAPRDVFIAVKTTKKFHRARLDLLLETWISRHKEMTFIFTDGEDEALARHTGNVVITNCSAAHSRQALSCKMAVEYDRFIESGRKWFCHVDDDNYVNLRALLRLLASYPHTRDVYVGKPSLDRPIQAMERVSENKVRPVHFWFATGGAGFCISRGLALKMSPWASGGHFMNTAERIRLPDDCTIGYIVEALLGVPLIRSGLFHSHLENLQQVPTSELHEQVTLSYGMFENKRNAVHVKGPFSVEADPSRFRSIHCHLYPDTPWCPRTAIF

Target ORF information:

RefSeq Version	NM_001040167.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (LFNG), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040167.2

ORF Insert Sequence:

ATGCTCAAGC GCTGCGGCCG GCGCCTGCTG CTGGCGCTGG CGGGCGCGCT GCTCGCCTGC 
CTGCTGGTGC TCACCGCCGA CCCGCCGCCG CCTCCACTGC CCGCCGAGCG CGGCCGGCGC 
GCGCTGCGCA GCCTGGCGGG CCCCGCGGGG GCTGCCCCGG CGCCCGGGCT GGGGGCGGCG 
GCGGCGGCGC CCGGGGCGCT GGTCCGCGAC GTGCACAGTC TGTCCGAGTA CTTCAGCCTG 
CTCACCCGCG CGCGCAGAGA TGCGGGCCCG CCGCCCGGGG CTGCCCCCCG CCCCGCCGAC 
GGCCACCCGC GCCCCCTGGC CGAGCCGCTC GCGCCCCGAG ACGTCTTCAT CGCTGTCAAG 
ACCACCAAAA AGTTCCACCG CGCGCGCCTC GACCTGCTGC TGGAGACCTG GATCTCGCGC 
CACAAGGAGA TGACGTTCAT CTTCACTGAC GGGGAAGATG AGGCCCTGGC CAGGCACACG 
GGCAACGTGG TCATCACAAA CTGCTCGGCC GCCCACAGCC GCCAGGCGCT GTCCTGCAAG 
ATGGCCGTGG AGTATGACCG CTTCATCGAG TCCGGCAGGA AGTGGTTCTG CCACGTGGAC 
GATGACAACT ACGTCAACCT GCGGGCCCTG CTGCGGCTGC TGGCCAGCTA CCCGCACACG 
CGGGACGTCT ACGTCGGCAA GCCCAGCCTG GACAGGCCCA TCCAGGCCAT GGAGCGGGTC 
AGCGAGAACA AGGTGCGTCC TGTCCACTTC TGGTTTGCCA CGGGCGGCGC TGGCTTCTGC 
ATCAGCCGTG GGCTGGCTCT GAAGATGAGC CCGTGGGCCA GCGGGGGTCA CTTCATGAAT 
ACGGCTGAGC GGATCCGGCT GCCTGATGAC TGCACCATCG GCTACATCGT GGAGGCCCTG 
CTGGGTGTGC CCCTCATCCG CAGCGGCCTC TTCCACTCCC ACCTGGAGAA CCTGCAGCAG 
GTGCCCACCT CGGAGCTCCA CGAGCAGGTG ACGCTGAGCT ACGGTATGTT TGAAAACAAG 
CGGAACGCCG TCCACGTGAA GGGGCCCTTC TCGGTGGAGG CCGACCCATC CAGGTTCCGC 
TCCATCCACT GCCACCTGTA CCCGGACACA CCCTGGTGTC CCCGCACTGC CATCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lunatic Fringe and p53 Cooperatively Suppress Mesenchymal Stem-Like Breast Cancer.
Neoplasia (New York, N.Y.)19(11)885-895(2017 Nov)
Chung WC,Zhang S,Challagundla L,Zhou Y,Xu K

Comparative genomics reveals that loss of lunatic fringe (LFNG) promotes melanoma metastasis.
Molecular oncology(2017 Nov)
Del Castillo Velasco-Herrera M,van der Weyden L,Nsengimana J,Speak AO,Sj?berg MK,Bishop DT,J?nsson G,Newton-Bishop J,Adams DJ

Reconstitution of TGFBR2 in HCT116 colorectal cancer cells causes increased LFNG expression and enhanced N-acetyl-d-glucosamine incorporation into Notch1.
Cellular signalling28(8)1105-13(2016 08)
Lee J,Katzenmaier EM,Kopitz J,Gebert J

Lunatic Fringe is a potent tumor suppressor in Kras-initiated pancreatic cancer.
Oncogene35(19)2485-95(2016 05)
Zhang S,Chung WC,Xu K

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LFNG cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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