LCT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LCT
Entrez Gene ID	3938
Full Name	lactase
Synonyms	LAC,LPH,LPH1
General protein information	Preferred Names lactase Names lactase-phlorizin hydrolase lactase-glycosylceramidase lactase-phlorizin hydrolase-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q21.3
Summary	The protein encoded by this gene belongs to the glycosyl hydrolase 1 family of proteins. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme is integral to the plasma membrane and has both phlorizin hydrolase activity and lactase activity. Mutations in this gene are associated with congenital lactase deficiency. Polymorphisms in this gene are associated with lactase persistence, in which intestinal lactase activity persists at childhood levels into adulthood. [provided by RefSeq, Jan 2016].
Disorder MIM:	603202
Disorder Html:	Lactase deficiency, congenital, 223000 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017004088.2	XP_016859577.1	lactase-phlorizin hydrolase isoform X1
NM_002299.4	NP_002290.2	lactase-phlorizin hydrolase preproprotein
XM_017004088.1	XP_016859577.1	lactase-phlorizin hydrolase isoform X1
NM_002299.2	NP_002290.2	lactase-phlorizin hydrolase preproprotein
NM_002299.3	NP_002290.2	lactase-phlorizin hydrolase preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LCT	XP_003954409.1
	LCT	XP_001096426.2
Canis lupus familiaris (dog)	LCT	XP_005632060.1
Mus musculus (house mouse)	Lct	NP_001074547.1
Danio rerio (zebrafish)	lct	XP_001336765.5
Xenopus tropicalis (tropical clawed frog)	LOC101732850	XP_004917683.1
Rattus norvegicus (Norway rat)	Lct	NP_446293.1
Gallus gallus (chicken)	LCT	NP_001104816.1
Xenopus tropicalis (tropical clawed frog)	lct.2	XP_002934014.2
Homo sapiens (human)	LCT	NP_002290.2
Bos taurus (cattle)	LCT	NP_001192716.1

Gene Ontology

The following LCT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LCT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18202	NM_002299.4 Latest version!	Homo sapiens lactase (LCT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu89445	XM_017004088.2 Latest version!	Homo sapiens lactase (LCT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OHu89445	XM_017004088.1	Homo sapiens lactase (LCT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu18202	NM_002299.2	Homo sapiens lactase (LCT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu18202	NM_002299.3	Homo sapiens lactase (LCT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LCT ( NM_002299.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002290.2 Homo sapiens lactase (LCT), mRNA.

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CloneID	OHu18202
Clone ID Related Accession (Same CDS sequence)	NM_002299.4 , NM_002299.2 , NM_002299.3
Accession Version	NM_002299.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	lactase-phlorizin hydrolase preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011893.7, X07994.1 and BX642122.1. On Nov 23, 2018 this sequence version replaced NM_002299.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X07994.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264162.7/ ENSP00000264162.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCTGT CTTGGCATGT AGTCTTTATT GCCCTGCTAA GTTTTTCATG CTGGGGGTCA 
GACTGGGAGT CTGATAGAAA TTTCATTTCC ACCGCTGGTC CTCTAACCAA TGACTTGCTG 
CACAACCTGA GTGGTCTCCT GGGAGACCAG AGTTCTAACT TTGTAGCAGG GGACAAAGAC 
ATGTATGTTT GTCACCAGCC ACTGCCCACT TTCCTGCCAG AATACTTCAG CAGTCTCCAT 
GCCAGTCAGA TCACCCATTA TAAGGTATTT CTGTCATGGG CACAGCTCCT CCCAGCAGGA 
AGCACCCAGA ATCCAGACGA GAAAACAGTG CAGTGCTACC GGCGACTCCT CAAGGCCCTC 
AAGACTGCAC GGCTTCAGCC CATGGTCATC CTGCACCACC AGACCCTCCC TGCCAGCACC 
CTCCGGAGAA CCGAAGCCTT TGCTGACCTC TTCGCCGACT ATGCCACATT CGCCTTCCAC 
TCCTTCGGGG ACCTAGTTGG GATCTGGTTC ACCTTCAGTG ACTTGGAGGA AGTGATCAAG 
GAGCTTCCCC ACCAGGAATC AAGAGCGTCA CAACTCCAGA CCCTCAGTGA TGCCCACAGA 
AAAGCCTATG AGATTTACCA CGAAAGCTAT GCTTTTCAGG GCGGAAAACT CTCTGTTGTC 
CTGCGAGCTG AAGATATCCC GGAGCTCCTG CTAGAACCAC CCATATCTGC GCTTGCCCAG 
GACACGGTCG ATTTCCTCTC TCTTGATTTG TCTTATGAAT GCCAAAATGA GGCAAGTCTG 
CGGCAGAAGC TGAGTAAATT GCAGACCATT GAGCCAAAAG TGAAAGTTTT CATCTTCAAC 
CTAAAACTCC CAGACTGCCC CTCCACCATG AAGAACCCAG CCAGTCTGCT CTTCAGCCTT 
TTTGAAGCCA TAAATAAAGA CCAAGTGCTC ACCATTGGGT TTGATATTAA TGAGTTTCTG 
AGTTGTTCAT CAAGTTCCAA GAAAAGCATG TCTTGTTCTC TGACTGGCAG CCTGGCCCTT 
CAGCCTGACC AGCAGCAGGA CCACGAGACC ACGGACTCCT CTCCTGCCTC TGCCTATCAG 
AGAATCTGGG AAGCATTTGC CAATCAGTCC AGGGCGGAAA GGGATGCCTT CCTGCAGGAT 
ACTTTCCCTG AAGGCTTCCT CTGGGGTGCC TCCACAGGAG CCTTTAACGT GGAAGGAGGC 
TGGGCCGAGG GTGGGAGAGG GGTGAGCATC TGGGATCCAC GCAGGCCCCT GAACACCACT 
GAGGGCCAAG CGACGCTGGA GGTGGCCAGC GACAGTTACC ACAAGGTAGC CTCTGACGTC 
GCCCTGCTTT GCGGCCTCCG GGCTCAGGTG TACAAGTTCT CCATCTCCTG GTCCCGGATC 
TTCCCCATGG GGCACGGGAG CAGCCCCAGC CTCCCAGGCG TTGCCTACTA CAACAAGCTG 
ATTGACAGGC TACAGGATGC GGGCATCGAG CCCATGGCCA CGCTGTTCCA CTGGGACCTG 
CCTCAGGCCC TGCAGGATCA TGGTGGATGG CAGAATGAGA GCGTGGTGGA TGCCTTCCTG 
GACTATGCGG CCTTCTGCTT CTCCACATTT GGGGACCGTG TGAAGCTGTG GGTGACCTTC 
CATGAGCCGT GGGTGATGAG CTACGCAGGC TATGGCACCG GCCAGCACCC TCCCGGCATC 
TCTGACCCAG GAGTGGCCTC TTTTAAGGTG GCTCACTTGG TCCTCAAGGC TCATGCCAGA 
ACTTGGCACC ACTACAACAG CCATCATCGC CCACAGCAGC AGGGGCACGT GGGCATTGTG 
CTGAACTCAG ACTGGGCAGA ACCCCTGTCT CCAGAGAGGC CTGAGGACCT GAGAGCCTCT 
GAGCGCTTCT TGCACTTCAT GCTGGGCTGG TTTGCACACC CCGTCTTTGT GGATGGAGAC 
TACCCAGCCA CCCTGAGGAC CCAGATCCAA CAGATGAACA GACAGTGCTC CCATCCTGTG 
GCTCAACTCC CCGAGTTCAC AGAGGCAGAG AAGCAGCTCC TGAAAGGCTC TGCTGATTTT 
CTGGGTCTGT CGCATTACAC CTCCCGCCTC ATCAGCAACG CCCCACAAAA CACCTGCATC 
CCTAGCTATG ATACCATTGG AGGCTTCTCC CAACACGTGA ACCATGTGTG GCCCCAGACC 
TCATCCTCTT GGATTCGTGT GGTGCCCTGG GGGATAAGGA GGCTGTTGCA GTTTGTATCC 
CTGGAATACA CAAGAGGAAA AGTTCCAATA TACCTTGCCG GGAATGGCAT GCCCATAGGG 
GAAAGTGAAA ATCTCTTTGA TGATTCCTTA AGAGTAGACT ACTTCAATCA ATATATCAAT 
GAGGTGCTCA AGGCTATCAA GGAAGACTCT GTGGATGTTC GTTCCTACAT TGCTCGTTCC 
CTCATTGATG GCTTCGAAGG CCCTTCTGGT TACAGCCAGC GGTTTGGCCT GCACCACGTC 
AACTTCAGCG ACAGCAGCAA GTCAAGGACT CCCAGGAAAT CTGCCTACTT TTTCACTAGC 
ATCATAGAAA AGAACGGTTT CCTCACCAAG GGGGCAAAAA GACTGCTACC ACCTAATACA 
GTAAACCTCC CCTCCAAAGT CAGAGCCTTC ACTTTTCCAT CTGAGGTGCC CTCCAAGGCT 
AAAGTCGTTT GGGAAAAGTT CTCCAGCCAA CCCAAGTTCG AAAGAGATTT GTTCTACCAC 
GGGACGTTTC GGGATGACTT TCTGTGGGGC GTGTCCTCTT CCGCTTATCA GATTGAAGGC 
GCGTGGGATG CCGATGGCAA AGGCCCCAGC ATCTGGGATA ACTTTACCCA CACACCAGGG 
AGCAATGTGA AAGACAATGC CACTGGAGAC ATCGCCTGTG ACAGCTATCA CCAGCTGGAT 
GCCGATCTGA ATATGCTCCG AGCTTTGAAG GTGAAGGCCT ACCGCTTCTC TATCTCCTGG 
TCTCGGATTT TCCCAACTGG GAGAAACAGC TCTATCAACA GTCATGGGGT TGATTATTAC 
AACAGGCTGA TCAATGGCTT GGTGGCAAGC AACATCTTTC CCATGGTGAC ATTGTTCCAT 
TGGGACCTGC CCCAGGCCCT CCAGGATATC GGAGGCTGGG AGAATCCTGC CTTGATTGAC 
TTGTTTGACA GCTACGCAGA CTTTTGTTTC CAGACCTTTG GTGATAGAGT CAAGTTTTGG 
ATGACTTTTA ATGAGCCCAT GTACCTGGCA TGGCTAGGTT ATGGCTCAGG GGAATTTCCC 
CCAGGGGTGA AGGACCCAGG CTGGGCACCA TATAGGATAG CCCACGCCGT CATCAAAGCC 
CATGCCAGAG TCTATCACAC GTACGATGAG AAATACAGGC AGGAGCAGAA GGGGGTCATC 
TCGCTGAGCC TCAGTACACA CTGGGCAGAG CCCAAGTCAC CAGGGGTCCC CAGAGATGTG 
GAAGCCGCTG ACCGAATGCT GCAGTTCTCC CTGGGCTGGT TTGCTCACCC CATTTTTAGA 
AACGGAGACT ATCCTGACAC CATGAAGTGG AAAGTGGGGA ACAGGAGTGA ACTGCAGCAC 
TTAGCCACCT CCCGCCTGCC AAGCTTCACT GAGGAAGAGA AGAGGTTCAT CAGGGCGACG 
GCCGACGTCT TCTGCCTCAA CACGTACTAC TCCAGAATCG TGCAGCACAA AACACCCAGG 
CTAAACCCAC CCTCCTACGA AGACGACCAG GAGATGGCTG AGGAGGAGGA CCCTTCGTGG 
CCTTCCACGG CAATGAACAG AGCTGCGCCC TGGGGGACGC GAAGGCTGCT GAACTGGATC 
AAGGAAGAGT ATGGTGACAT CCCCATTTAC ATCACCGAAA ACGGAGTGGG GCTGACCAAT 
CCGAACACGG AGGATACTGA TAGGATATTT TACCACAAAA CCTACATCAA TGAGGCTTTG 
AAAGCCTACA GGCTCGATGG TATAGACCTT CGAGGGTATG TCGCCTGGTC TCTGATGGAC 
AACTTTGAGT GGCTAAATGG CTACACGGTC AAGTTTGGAC TGTACCATGT TGATTTCAAC 
AACACGAACA GGCCTCGCAC AGCAAGAGCC TCCGCCAGGT ACTACACAGA GGTCATTACC 
AACAACGGCA TGCCACTGGC CAGGGAGGAT GAGTTTCTGT ACGGACGGTT TCCTGAGGGC 
TTCATCTGGA GTGCAGCTTC TGCTGCATAT CAGATTGAAG GTGCGTGGAG AGCAGATGGC 
AAAGGACTCA GCATTTGGGA CACGTTTTCT CACACACCAC TGAGGGTTGA GAACGATGCC 
ATTGGAGACG TGGCCTGTGA CAGTTATCAC AAGATTGCTG AGGATCTGGT CACCCTGCAG 
AACCTGGGCG TGTCCCACTA CCGTTTTTCC ATCTCCTGGT CTCGCATCCT CCCTGATGGA 
ACCACCAGGT ACATCAATGA AGCGGGCCTG AACTACTACG TGAGGCTCAT CGATACACTG 
CTGGCCGCCA GCATCCAGCC CCAGGTGACC ATTTACCACT GGGACCTACC ACAGACGCTC 
CAAGATGTAG GAGGCTGGGA GAATGAGACC ATCGTGCAGC GGTTTAAGGA GTATGCAGAT 
GTGCTCTTCC AGAGGCTGGG AGACAAGGTG AAGTTTTGGA TCACGCTGAA TGAGCCCTTT 
GTCATTGCTT ACCAGGGCTA TGGCTACGGA ACAGCAGCTC CAGGAGTCTC CAATAGGCCT 
GGCACTGCCC CCTACATTGT TGGCCACAAT CTAATAAAGG CTCATGCTGA GGCCTGGCAT 
CTGTACAACG ATGTGTACCG CGCCAGTCAA GGTGGCGTGA TTTCCATCAC CATCAGCAGT 
GACTGGGCTG AACCCAGAGA TCCCTCTAAC CAGGAGGATG TGGAGGCAGC CAGGAGATAT 
GTTCAGTTCA TGGGAGGCTG GTTTGCACAT CCTATTTTCA AGAATGGAGA TTACAATGAG 
GTGATGAAGA CGCGGATCCG TGACAGGAGC TTGGCTGCAG GCCTCAACAA GTCTCGGCTG 
CCAGAATTTA CAGAGAGTGA GAAGAGGAGG ATCAACGGCA CCTATGACTT TTTTGGGTTC 
AATCACTACA CCACTGTCCT CGCCTACAAC CTCAACTATG CCACTGCCAT CTCTTCTTTT 
GATGCAGACA GAGGAGTTGC TTCCATCGCA GATCGCTCGT GGCCAGACTC TGGCTCCTTC 
TGGCTGAAGA TGACGCCTTT TGGCTTCAGG AGGATCCTGA ACTGGTTAAA GGAGGAATAC 
AATGACCCTC CAATTTATGT CACAGAGAAT GGAGTGTCCC AGCGGGAAGA AACAGACCTC 
AATGACACTG CAAGGATCTA CTACCTTCGG ACTTACATCA ATGAGGCCCT CAAAGCTGTG 
CAGGACAAGG TGGACCTTCG AGGATACACA GTTTGGAGTG CGATGGACAA TTTTGAGTGG 
GCCACAGGCT TTTCAGAGAG ATTTGGTCTG CATTTTGTGA ACTACAGTGA CCCTTCTCTG 
CCAAGGATCC CCAAAGCATC AGCGAAGTTC TACGCCTCTG TGGTCCGATG CAATGGCTTC 
CCTGACCCCG CTACAGGGCC TCACGCTTGT CTCCACCAGC CAGATGCTGG ACCCACCATC 
AGCCCCGTGA GACAGGAGGA GGTGCAGTTC CTGGGGCTAA TGCTCGGCAC CACAGAAGCA 
CAGACAGCTT TGTACGTTCT CTTTTCTCTT GTGCTTCTTG GAGTCTGTGG CTTGGCATTT 
CTGTCATACA AGTACTGCAA GCGCTCTAAG CAAGGGAAAA CACAACGAAG CCAACAGGAA 
TTGAGCCCGG TGTCTTCATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002290.2
CDS	16..5799
Translation	MELSWHVVFIALLSFSCWGSDWESDRNFISTAGPLTNDLLHNLSGLLGDQSSNFVAGDKDMYVCHQPLPTFLPEYFSSLHASQITHYKVFLSWAQLLPAGSTQNPDEKTVQCYRRLLKALKTARLQPMVILHHQTLPASTLRRTEAFADLFADYATFAFHSFGDLVGIWFTFSDLEEVIKELPHQESRASQLQTLSDAHRKAYEIYHESYAFQGGKLSVVLRAEDIPELLLEPPISALAQDTVDFLSLDLSYECQNEASLRQKLSKLQTIEPKVKVFIFNLKLPDCPSTMKNPASLLFSLFEAINKDQVLTIGFDINEFLSCSSSSKKSMSCSLTGSLALQPDQQQDHETTDSSPASAYQRIWEAFANQSRAERDAFLQDTFPEGFLWGASTGAFNVEGGWAEGGRGVSIWDPRRPLNTTEGQATLEVASDSYHKVASDVALLCGLRAQVYKFSISWSRIFPMGHGSSPSLPGVAYYNKLIDRLQDAGIEPMATLFHWDLPQALQDHGGWQNESVVDAFLDYAAFCFSTFGDRVKLWVTFHEPWVMSYAGYGTGQHPPGISDPGVASFKVAHLVLKAHARTWHHYNSHHRPQQQGHVGIVLNSDWAEPLSPERPEDLRASERFLHFMLGWFAHPVFVDGDYPATLRTQIQQMNRQCSHPVAQLPEFTEAEKQLLKGSADFLGLSHYTSRLISNAPQNTCIPSYDTIGGFSQHVNHVWPQTSSSWIRVVPWGIRRLLQFVSLEYTRGKVPIYLAGNGMPIGESENLFDDSLRVDYFNQYINEVLKAIKEDSVDVRSYIARSLIDGFEGPSGYSQRFGLHHVNFSDSSKSRTPRKSAYFFTSIIEKNGFLTKGAKRLLPPNTVNLPSKVRAFTFPSEVPSKAKVVWEKFSSQPKFERDLFYHGTFRDDFLWGVSSSAYQIEGAWDADGKGPSIWDNFTHTPGSNVKDNATGDIACDSYHQLDADLNMLRALKVKAYRFSISWSRIFPTGRNSSINSHGVDYYNRLINGLVASNIFPMVTLFHWDLPQALQDIGGWENPALIDLFDSYADFCFQTFGDRVKFWMTFNEPMYLAWLGYGSGEFPPGVKDPGWAPYRIAHAVIKAHARVYHTYDEKYRQEQKGVISLSLSTHWAEPKSPGVPRDVEAADRMLQFSLGWFAHPIFRNGDYPDTMKWKVGNRSELQHLATSRLPSFTEEEKRFIRATADVFCLNTYYSRIVQHKTPRLNPPSYEDDQEMAEEEDPSWPSTAMNRAAPWGTRRLLNWIKEEYGDIPIYITENGVGLTNPNTEDTDRIFYHKTYINEALKAYRLDGIDLRGYVAWSLMDNFEWLNGYTVKFGLYHVDFNNTNRPRTARASARYYTEVITNNGMPLAREDEFLYGRFPEGFIWSAASAAYQIEGAWRADGKGLSIWDTFSHTPLRVENDAIGDVACDSYHKIAEDLVTLQNLGVSHYRFSISWSRILPDGTTRYINEAGLNYYVRLIDTLLAASIQPQVTIYHWDLPQTLQDVGGWENETIVQRFKEYADVLFQRLGDKVKFWITLNEPFVIAYQGYGYGTAAPGVSNRPGTAPYIVGHNLIKAHAEAWHLYNDVYRASQGGVISITISSDWAEPRDPSNQEDVEAARRYVQFMGGWFAHPIFKNGDYNEVMKTRIRDRSLAAGLNKSRLPEFTESEKRRINGTYDFFGFNHYTTVLAYNLNYATAISSFDADRGVASIADRSWPDSGSFWLKMTPFGFRRILNWLKEEYNDPPIYVTENGVSQREETDLNDTARIYYLRTYINEALKAVQDKVDLRGYTVWSAMDNFEWATGFSERFGLHFVNYSDPSLPRIPKASAKFYASVVRCNGFPDPATGPHACLHQPDAGPTISPVRQEEVQFLGLMLGTTEAQTALYVLFSLVLLGVCGLAFLSYKYCKRSKQGKTQRSQQELSPVSSF

Target ORF information:

RefSeq Version	NM_002299.4
Organism	Homo sapiens(human)
Definition	Homo sapiens lactase (LCT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002299.4

ORF Insert Sequence:

ATGGAGCTGT CTTGGCATGT AGTCTTTATT GCCCTGCTAA GTTTTTCATG CTGGGGGTCA 
GACTGGGAGT CTGATAGAAA TTTCATTTCC ACCGCTGGTC CTCTAACCAA TGACTTGCTG 
CACAACCTGA GTGGTCTCCT GGGAGACCAG AGTTCTAACT TTGTAGCAGG GGACAAAGAC 
ATGTATGTTT GTCACCAGCC ACTGCCCACT TTCCTGCCAG AATACTTCAG CAGTCTCCAT 
GCCAGTCAGA TCACCCATTA TAAGGTATTT CTGTCATGGG CACAGCTCCT CCCAGCAGGA 
AGCACCCAGA ATCCAGACGA GAAAACAGTG CAGTGCTACC GGCGACTCCT CAAGGCCCTC 
AAGACTGCAC GGCTTCAGCC CATGGTCATC CTGCACCACC AGACCCTCCC TGCCAGCACC 
CTCCGGAGAA CCGAAGCCTT TGCTGACCTC TTCGCCGACT ATGCCACATT CGCCTTCCAC 
TCCTTCGGGG ACCTAGTTGG GATCTGGTTC ACCTTCAGTG ACTTGGAGGA AGTGATCAAG 
GAGCTTCCCC ACCAGGAATC AAGAGCGTCA CAACTCCAGA CCCTCAGTGA TGCCCACAGA 
AAAGCCTATG AGATTTACCA CGAAAGCTAT GCTTTTCAGG GCGGAAAACT CTCTGTTGTC 
CTGCGAGCTG AAGATATCCC GGAGCTCCTG CTAGAACCAC CCATATCTGC GCTTGCCCAG 
GACACGGTCG ATTTCCTCTC TCTTGATTTG TCTTATGAAT GCCAAAATGA GGCAAGTCTG 
CGGCAGAAGC TGAGTAAATT GCAGACCATT GAGCCAAAAG TGAAAGTTTT CATCTTCAAC 
CTAAAACTCC CAGACTGCCC CTCCACCATG AAGAACCCAG CCAGTCTGCT CTTCAGCCTT 
TTTGAAGCCA TAAATAAAGA CCAAGTGCTC ACCATTGGGT TTGATATTAA TGAGTTTCTG 
AGTTGTTCAT CAAGTTCCAA GAAAAGCATG TCTTGTTCTC TGACTGGCAG CCTGGCCCTT 
CAGCCTGACC AGCAGCAGGA CCACGAGACC ACGGACTCCT CTCCTGCCTC TGCCTATCAG 
AGAATCTGGG AAGCATTTGC CAATCAGTCC AGGGCGGAAA GGGATGCCTT CCTGCAGGAT 
ACTTTCCCTG AAGGCTTCCT CTGGGGTGCC TCCACAGGAG CCTTTAACGT GGAAGGAGGC 
TGGGCCGAGG GTGGGAGAGG GGTGAGCATC TGGGATCCAC GCAGGCCCCT GAACACCACT 
GAGGGCCAAG CGACGCTGGA GGTGGCCAGC GACAGTTACC ACAAGGTAGC CTCTGACGTC 
GCCCTGCTTT GCGGCCTCCG GGCTCAGGTG TACAAGTTCT CCATCTCCTG GTCCCGGATC 
TTCCCCATGG GGCACGGGAG CAGCCCCAGC CTCCCAGGCG TTGCCTACTA CAACAAGCTG 
ATTGACAGGC TACAGGATGC GGGCATCGAG CCCATGGCCA CGCTGTTCCA CTGGGACCTG 
CCTCAGGCCC TGCAGGATCA TGGTGGATGG CAGAATGAGA GCGTGGTGGA TGCCTTCCTG 
GACTATGCGG CCTTCTGCTT CTCCACATTT GGGGACCGTG TGAAGCTGTG GGTGACCTTC 
CATGAGCCGT GGGTGATGAG CTACGCAGGC TATGGCACCG GCCAGCACCC TCCCGGCATC 
TCTGACCCAG GAGTGGCCTC TTTTAAGGTG GCTCACTTGG TCCTCAAGGC TCATGCCAGA 
ACTTGGCACC ACTACAACAG CCATCATCGC CCACAGCAGC AGGGGCACGT GGGCATTGTG 
CTGAACTCAG ACTGGGCAGA ACCCCTGTCT CCAGAGAGGC CTGAGGACCT GAGAGCCTCT 
GAGCGCTTCT TGCACTTCAT GCTGGGCTGG TTTGCACACC CCGTCTTTGT GGATGGAGAC 
TACCCAGCCA CCCTGAGGAC CCAGATCCAA CAGATGAACA GACAGTGCTC CCATCCTGTG 
GCTCAACTCC CCGAGTTCAC AGAGGCAGAG AAGCAGCTCC TGAAAGGCTC TGCTGATTTT 
CTGGGTCTGT CGCATTACAC CTCCCGCCTC ATCAGCAACG CCCCACAAAA CACCTGCATC 
CCTAGCTATG ATACCATTGG AGGCTTCTCC CAACACGTGA ACCATGTGTG GCCCCAGACC 
TCATCCTCTT GGATTCGTGT GGTGCCCTGG GGGATAAGGA GGCTGTTGCA GTTTGTATCC 
CTGGAATACA CAAGAGGAAA AGTTCCAATA TACCTTGCCG GGAATGGCAT GCCCATAGGG 
GAAAGTGAAA ATCTCTTTGA TGATTCCTTA AGAGTAGACT ACTTCAATCA ATATATCAAT 
GAGGTGCTCA AGGCTATCAA GGAAGACTCT GTGGATGTTC GTTCCTACAT TGCTCGTTCC 
CTCATTGATG GCTTCGAAGG CCCTTCTGGT TACAGCCAGC GGTTTGGCCT GCACCACGTC 
AACTTCAGCG ACAGCAGCAA GTCAAGGACT CCCAGGAAAT CTGCCTACTT TTTCACTAGC 
ATCATAGAAA AGAACGGTTT CCTCACCAAG GGGGCAAAAA GACTGCTACC ACCTAATACA 
GTAAACCTCC CCTCCAAAGT CAGAGCCTTC ACTTTTCCAT CTGAGGTGCC CTCCAAGGCT 
AAAGTCGTTT GGGAAAAGTT CTCCAGCCAA CCCAAGTTCG AAAGAGATTT GTTCTACCAC 
GGGACGTTTC GGGATGACTT TCTGTGGGGC GTGTCCTCTT CCGCTTATCA GATTGAAGGC 
GCGTGGGATG CCGATGGCAA AGGCCCCAGC ATCTGGGATA ACTTTACCCA CACACCAGGG 
AGCAATGTGA AAGACAATGC CACTGGAGAC ATCGCCTGTG ACAGCTATCA CCAGCTGGAT 
GCCGATCTGA ATATGCTCCG AGCTTTGAAG GTGAAGGCCT ACCGCTTCTC TATCTCCTGG 
TCTCGGATTT TCCCAACTGG GAGAAACAGC TCTATCAACA GTCATGGGGT TGATTATTAC 
AACAGGCTGA TCAATGGCTT GGTGGCAAGC AACATCTTTC CCATGGTGAC ATTGTTCCAT 
TGGGACCTGC CCCAGGCCCT CCAGGATATC GGAGGCTGGG AGAATCCTGC CTTGATTGAC 
TTGTTTGACA GCTACGCAGA CTTTTGTTTC CAGACCTTTG GTGATAGAGT CAAGTTTTGG 
ATGACTTTTA ATGAGCCCAT GTACCTGGCA TGGCTAGGTT ATGGCTCAGG GGAATTTCCC 
CCAGGGGTGA AGGACCCAGG CTGGGCACCA TATAGGATAG CCCACGCCGT CATCAAAGCC 
CATGCCAGAG TCTATCACAC GTACGATGAG AAATACAGGC AGGAGCAGAA GGGGGTCATC 
TCGCTGAGCC TCAGTACACA CTGGGCAGAG CCCAAGTCAC CAGGGGTCCC CAGAGATGTG 
GAAGCCGCTG ACCGAATGCT GCAGTTCTCC CTGGGCTGGT TTGCTCACCC CATTTTTAGA 
AACGGAGACT ATCCTGACAC CATGAAGTGG AAAGTGGGGA ACAGGAGTGA ACTGCAGCAC 
TTAGCCACCT CCCGCCTGCC AAGCTTCACT GAGGAAGAGA AGAGGTTCAT CAGGGCGACG 
GCCGACGTCT TCTGCCTCAA CACGTACTAC TCCAGAATCG TGCAGCACAA AACACCCAGG 
CTAAACCCAC CCTCCTACGA AGACGACCAG GAGATGGCTG AGGAGGAGGA CCCTTCGTGG 
CCTTCCACGG CAATGAACAG AGCTGCGCCC TGGGGGACGC GAAGGCTGCT GAACTGGATC 
AAGGAAGAGT ATGGTGACAT CCCCATTTAC ATCACCGAAA ACGGAGTGGG GCTGACCAAT 
CCGAACACGG AGGATACTGA TAGGATATTT TACCACAAAA CCTACATCAA TGAGGCTTTG 
AAAGCCTACA GGCTCGATGG TATAGACCTT CGAGGGTATG TCGCCTGGTC TCTGATGGAC 
AACTTTGAGT GGCTAAATGG CTACACGGTC AAGTTTGGAC TGTACCATGT TGATTTCAAC 
AACACGAACA GGCCTCGCAC AGCAAGAGCC TCCGCCAGGT ACTACACAGA GGTCATTACC 
AACAACGGCA TGCCACTGGC CAGGGAGGAT GAGTTTCTGT ACGGACGGTT TCCTGAGGGC 
TTCATCTGGA GTGCAGCTTC TGCTGCATAT CAGATTGAAG GTGCGTGGAG AGCAGATGGC 
AAAGGACTCA GCATTTGGGA CACGTTTTCT CACACACCAC TGAGGGTTGA GAACGATGCC 
ATTGGAGACG TGGCCTGTGA CAGTTATCAC AAGATTGCTG AGGATCTGGT CACCCTGCAG 
AACCTGGGCG TGTCCCACTA CCGTTTTTCC ATCTCCTGGT CTCGCATCCT CCCTGATGGA 
ACCACCAGGT ACATCAATGA AGCGGGCCTG AACTACTACG TGAGGCTCAT CGATACACTG 
CTGGCCGCCA GCATCCAGCC CCAGGTGACC ATTTACCACT GGGACCTACC ACAGACGCTC 
CAAGATGTAG GAGGCTGGGA GAATGAGACC ATCGTGCAGC GGTTTAAGGA GTATGCAGAT 
GTGCTCTTCC AGAGGCTGGG AGACAAGGTG AAGTTTTGGA TCACGCTGAA TGAGCCCTTT 
GTCATTGCTT ACCAGGGCTA TGGCTACGGA ACAGCAGCTC CAGGAGTCTC CAATAGGCCT 
GGCACTGCCC CCTACATTGT TGGCCACAAT CTAATAAAGG CTCATGCTGA GGCCTGGCAT 
CTGTACAACG ATGTGTACCG CGCCAGTCAA GGTGGCGTGA TTTCCATCAC CATCAGCAGT 
GACTGGGCTG AACCCAGAGA TCCCTCTAAC CAGGAGGATG TGGAGGCAGC CAGGAGATAT 
GTTCAGTTCA TGGGAGGCTG GTTTGCACAT CCTATTTTCA AGAATGGAGA TTACAATGAG 
GTGATGAAGA CGCGGATCCG TGACAGGAGC TTGGCTGCAG GCCTCAACAA GTCTCGGCTG 
CCAGAATTTA CAGAGAGTGA GAAGAGGAGG ATCAACGGCA CCTATGACTT TTTTGGGTTC 
AATCACTACA CCACTGTCCT CGCCTACAAC CTCAACTATG CCACTGCCAT CTCTTCTTTT 
GATGCAGACA GAGGAGTTGC TTCCATCGCA GATCGCTCGT GGCCAGACTC TGGCTCCTTC 
TGGCTGAAGA TGACGCCTTT TGGCTTCAGG AGGATCCTGA ACTGGTTAAA GGAGGAATAC 
AATGACCCTC CAATTTATGT CACAGAGAAT GGAGTGTCCC AGCGGGAAGA AACAGACCTC 
AATGACACTG CAAGGATCTA CTACCTTCGG ACTTACATCA ATGAGGCCCT CAAAGCTGTG 
CAGGACAAGG TGGACCTTCG AGGATACACA GTTTGGAGTG CGATGGACAA TTTTGAGTGG 
GCCACAGGCT TTTCAGAGAG ATTTGGTCTG CATTTTGTGA ACTACAGTGA CCCTTCTCTG 
CCAAGGATCC CCAAAGCATC AGCGAAGTTC TACGCCTCTG TGGTCCGATG CAATGGCTTC 
CCTGACCCCG CTACAGGGCC TCACGCTTGT CTCCACCAGC CAGATGCTGG ACCCACCATC 
AGCCCCGTGA GACAGGAGGA GGTGCAGTTC CTGGGGCTAA TGCTCGGCAC CACAGAAGCA 
CAGACAGCTT TGTACGTTCT CTTTTCTCTT GTGCTTCTTG GAGTCTGTGG CTTGGCATTT 
CTGTCATACA AGTACTGCAA GCGCTCTAAG CAAGGGAAAA CACAACGAAG CCAACAGGAA 
TTGAGCCCGG TGTCTTCATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LCT ( NM_002299.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002290.2 Homo sapiens lactase (LCT), mRNA.

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CloneID	OHu18202
Clone ID Related Accession (Same CDS sequence)	NM_002299.4 , NM_002299.2 , NM_002299.3
Accession Version	NM_002299.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	lactase-phlorizin hydrolase preproprotein
Comment

ATGGAGCTGT CTTGGCATGT AGTCTTTATT GCCCTGCTAA GTTTTTCATG CTGGGGGTCA 
GACTGGGAGT CTGATAGAAA TTTCATTTCC ACCGCTGGTC CTCTAACCAA TGACTTGCTG 
CACAACCTGA GTGGTCTCCT GGGAGACCAG AGTTCTAACT TTGTAGCAGG GGACAAAGAC 
ATGTATGTTT GTCACCAGCC ACTGCCCACT TTCCTGCCAG AATACTTCAG CAGTCTCCAT 
GCCAGTCAGA TCACCCATTA TAAGGTATTT CTGTCATGGG CACAGCTCCT CCCAGCAGGA 
AGCACCCAGA ATCCAGACGA GAAAACAGTG CAGTGCTACC GGCGACTCCT CAAGGCCCTC 
AAGACTGCAC GGCTTCAGCC CATGGTCATC CTGCACCACC AGACCCTCCC TGCCAGCACC 
CTCCGGAGAA CCGAAGCCTT TGCTGACCTC TTCGCCGACT ATGCCACATT CGCCTTCCAC 
TCCTTCGGGG ACCTAGTTGG GATCTGGTTC ACCTTCAGTG ACTTGGAGGA AGTGATCAAG 
GAGCTTCCCC ACCAGGAATC AAGAGCGTCA CAACTCCAGA CCCTCAGTGA TGCCCACAGA 
AAAGCCTATG AGATTTACCA CGAAAGCTAT GCTTTTCAGG GCGGAAAACT CTCTGTTGTC 
CTGCGAGCTG AAGATATCCC GGAGCTCCTG CTAGAACCAC CCATATCTGC GCTTGCCCAG 
GACACGGTCG ATTTCCTCTC TCTTGATTTG TCTTATGAAT GCCAAAATGA GGCAAGTCTG 
CGGCAGAAGC TGAGTAAATT GCAGACCATT GAGCCAAAAG TGAAAGTTTT CATCTTCAAC 
CTAAAACTCC CAGACTGCCC CTCCACCATG AAGAACCCAG CCAGTCTGCT CTTCAGCCTT 
TTTGAAGCCA TAAATAAAGA CCAAGTGCTC ACCATTGGGT TTGATATTAA TGAGTTTCTG 
AGTTGTTCAT CAAGTTCCAA GAAAAGCATG TCTTGTTCTC TGACTGGCAG CCTGGCCCTT 
CAGCCTGACC AGCAGCAGGA CCACGAGACC ACGGACTCCT CTCCTGCCTC TGCCTATCAG 
AGAATCTGGG AAGCATTTGC CAATCAGTCC AGGGCGGAAA GGGATGCCTT CCTGCAGGAT 
ACTTTCCCTG AAGGCTTCCT CTGGGGTGCC TCCACAGGAG CCTTTAACGT GGAAGGAGGC 
TGGGCCGAGG GTGGGAGAGG GGTGAGCATC TGGGATCCAC GCAGGCCCCT GAACACCACT 
GAGGGCCAAG CGACGCTGGA GGTGGCCAGC GACAGTTACC ACAAGGTAGC CTCTGACGTC 
GCCCTGCTTT GCGGCCTCCG GGCTCAGGTG TACAAGTTCT CCATCTCCTG GTCCCGGATC 
TTCCCCATGG GGCACGGGAG CAGCCCCAGC CTCCCAGGCG TTGCCTACTA CAACAAGCTG 
ATTGACAGGC TACAGGATGC GGGCATCGAG CCCATGGCCA CGCTGTTCCA CTGGGACCTG 
CCTCAGGCCC TGCAGGATCA TGGTGGATGG CAGAATGAGA GCGTGGTGGA TGCCTTCCTG 
GACTATGCGG CCTTCTGCTT CTCCACATTT GGGGACCGTG TGAAGCTGTG GGTGACCTTC 
CATGAGCCGT GGGTGATGAG CTACGCAGGC TATGGCACCG GCCAGCACCC TCCCGGCATC 
TCTGACCCAG GAGTGGCCTC TTTTAAGGTG GCTCACTTGG TCCTCAAGGC TCATGCCAGA 
ACTTGGCACC ACTACAACAG CCATCATCGC CCACAGCAGC AGGGGCACGT GGGCATTGTG 
CTGAACTCAG ACTGGGCAGA ACCCCTGTCT CCAGAGAGGC CTGAGGACCT GAGAGCCTCT 
GAGCGCTTCT TGCACTTCAT GCTGGGCTGG TTTGCACACC CCGTCTTTGT GGATGGAGAC 
TACCCAGCCA CCCTGAGGAC CCAGATCCAA CAGATGAACA GACAGTGCTC CCATCCTGTG 
GCTCAACTCC CCGAGTTCAC AGAGGCAGAG AAGCAGCTCC TGAAAGGCTC TGCTGATTTT 
CTGGGTCTGT CGCATTACAC CTCCCGCCTC ATCAGCAACG CCCCACAAAA CACCTGCATC 
CCTAGCTATG ATACCATTGG AGGCTTCTCC CAACACGTGA ACCATGTGTG GCCCCAGACC 
TCATCCTCTT GGATTCGTGT GGTGCCCTGG GGGATAAGGA GGCTGTTGCA GTTTGTATCC 
CTGGAATACA CAAGAGGAAA AGTTCCAATA TACCTTGCCG GGAATGGCAT GCCCATAGGG 
GAAAGTGAAA ATCTCTTTGA TGATTCCTTA AGAGTAGACT ACTTCAATCA ATATATCAAT 
GAGGTGCTCA AGGCTATCAA GGAAGACTCT GTGGATGTTC GTTCCTACAT TGCTCGTTCC 
CTCATTGATG GCTTCGAAGG CCCTTCTGGT TACAGCCAGC GGTTTGGCCT GCACCACGTC 
AACTTCAGCG ACAGCAGCAA GTCAAGGACT CCCAGGAAAT CTGCCTACTT TTTCACTAGC 
ATCATAGAAA AGAACGGTTT CCTCACCAAG GGGGCAAAAA GACTGCTACC ACCTAATACA 
GTAAACCTCC CCTCCAAAGT CAGAGCCTTC ACTTTTCCAT CTGAGGTGCC CTCCAAGGCT 
AAAGTCGTTT GGGAAAAGTT CTCCAGCCAA CCCAAGTTCG AAAGAGATTT GTTCTACCAC 
GGGACGTTTC GGGATGACTT TCTGTGGGGC GTGTCCTCTT CCGCTTATCA GATTGAAGGC 
GCGTGGGATG CCGATGGCAA AGGCCCCAGC ATCTGGGATA ACTTTACCCA CACACCAGGG 
AGCAATGTGA AAGACAATGC CACTGGAGAC ATCGCCTGTG ACAGCTATCA CCAGCTGGAT 
GCCGATCTGA ATATGCTCCG AGCTTTGAAG GTGAAGGCCT ACCGCTTCTC TATCTCCTGG 
TCTCGGATTT TCCCAACTGG GAGAAACAGC TCTATCAACA GTCATGGGGT TGATTATTAC 
AACAGGCTGA TCAATGGCTT GGTGGCAAGC AACATCTTTC CCATGGTGAC ATTGTTCCAT 
TGGGACCTGC CCCAGGCCCT CCAGGATATC GGAGGCTGGG AGAATCCTGC CTTGATTGAC 
TTGTTTGACA GCTACGCAGA CTTTTGTTTC CAGACCTTTG GTGATAGAGT CAAGTTTTGG 
ATGACTTTTA ATGAGCCCAT GTACCTGGCA TGGCTAGGTT ATGGCTCAGG GGAATTTCCC 
CCAGGGGTGA AGGACCCAGG CTGGGCACCA TATAGGATAG CCCACGCCGT CATCAAAGCC 
CATGCCAGAG TCTATCACAC GTACGATGAG AAATACAGGC AGGAGCAGAA GGGGGTCATC 
TCGCTGAGCC TCAGTACACA CTGGGCAGAG CCCAAGTCAC CAGGGGTCCC CAGAGATGTG 
GAAGCCGCTG ACCGAATGCT GCAGTTCTCC CTGGGCTGGT TTGCTCACCC CATTTTTAGA 
AACGGAGACT ATCCTGACAC CATGAAGTGG AAAGTGGGGA ACAGGAGTGA ACTGCAGCAC 
TTAGCCACCT CCCGCCTGCC AAGCTTCACT GAGGAAGAGA AGAGGTTCAT CAGGGCGACG 
GCCGACGTCT TCTGCCTCAA CACGTACTAC TCCAGAATCG TGCAGCACAA AACACCCAGG 
CTAAACCCAC CCTCCTACGA AGACGACCAG GAGATGGCTG AGGAGGAGGA CCCTTCGTGG 
CCTTCCACGG CAATGAACAG AGCTGCGCCC TGGGGGACGC GAAGGCTGCT GAACTGGATC 
AAGGAAGAGT ATGGTGACAT CCCCATTTAC ATCACCGAAA ACGGAGTGGG GCTGACCAAT 
CCGAACACGG AGGATACTGA TAGGATATTT TACCACAAAA CCTACATCAA TGAGGCTTTG 
AAAGCCTACA GGCTCGATGG TATAGACCTT CGAGGGTATG TCGCCTGGTC TCTGATGGAC 
AACTTTGAGT GGCTAAATGG CTACACGGTC AAGTTTGGAC TGTACCATGT TGATTTCAAC 
AACACGAACA GGCCTCGCAC AGCAAGAGCC TCCGCCAGGT ACTACACAGA GGTCATTACC 
AACAACGGCA TGCCACTGGC CAGGGAGGAT GAGTTTCTGT ACGGACGGTT TCCTGAGGGC 
TTCATCTGGA GTGCAGCTTC TGCTGCATAT CAGATTGAAG GTGCGTGGAG AGCAGATGGC 
AAAGGACTCA GCATTTGGGA CACGTTTTCT CACACACCAC TGAGGGTTGA GAACGATGCC 
ATTGGAGACG TGGCCTGTGA CAGTTATCAC AAGATTGCTG AGGATCTGGT CACCCTGCAG 
AACCTGGGCG TGTCCCACTA CCGTTTTTCC ATCTCCTGGT CTCGCATCCT CCCTGATGGA 
ACCACCAGGT ACATCAATGA AGCGGGCCTG AACTACTACG TGAGGCTCAT CGATACACTG 
CTGGCCGCCA GCATCCAGCC CCAGGTGACC ATTTACCACT GGGACCTACC ACAGACGCTC 
CAAGATGTAG GAGGCTGGGA GAATGAGACC ATCGTGCAGC GGTTTAAGGA GTATGCAGAT 
GTGCTCTTCC AGAGGCTGGG AGACAAGGTG AAGTTTTGGA TCACGCTGAA TGAGCCCTTT 
GTCATTGCTT ACCAGGGCTA TGGCTACGGA ACAGCAGCTC CAGGAGTCTC CAATAGGCCT 
GGCACTGCCC CCTACATTGT TGGCCACAAT CTAATAAAGG CTCATGCTGA GGCCTGGCAT 
CTGTACAACG ATGTGTACCG CGCCAGTCAA GGTGGCGTGA TTTCCATCAC CATCAGCAGT 
GACTGGGCTG AACCCAGAGA TCCCTCTAAC CAGGAGGATG TGGAGGCAGC CAGGAGATAT 
GTTCAGTTCA TGGGAGGCTG GTTTGCACAT CCTATTTTCA AGAATGGAGA TTACAATGAG 
GTGATGAAGA CGCGGATCCG TGACAGGAGC TTGGCTGCAG GCCTCAACAA GTCTCGGCTG 
CCAGAATTTA CAGAGAGTGA GAAGAGGAGG ATCAACGGCA CCTATGACTT TTTTGGGTTC 
AATCACTACA CCACTGTCCT CGCCTACAAC CTCAACTATG CCACTGCCAT CTCTTCTTTT 
GATGCAGACA GAGGAGTTGC TTCCATCGCA GATCGCTCGT GGCCAGACTC TGGCTCCTTC 
TGGCTGAAGA TGACGCCTTT TGGCTTCAGG AGGATCCTGA ACTGGTTAAA GGAGGAATAC 
AATGACCCTC CAATTTATGT CACAGAGAAT GGAGTGTCCC AGCGGGAAGA AACAGACCTC 
AATGACACTG CAAGGATCTA CTACCTTCGG ACTTACATCA ATGAGGCCCT CAAAGCTGTG 
CAGGACAAGG TGGACCTTCG AGGATACACA GTTTGGAGTG CGATGGACAA TTTTGAGTGG 
GCCACAGGCT TTTCAGAGAG ATTTGGTCTG CATTTTGTGA ACTACAGTGA CCCTTCTCTG 
CCAAGGATCC CCAAAGCATC AGCGAAGTTC TACGCCTCTG TGGTCCGATG CAATGGCTTC 
CCTGACCCCG CTACAGGGCC TCACGCTTGT CTCCACCAGC CAGATGCTGG ACCCACCATC 
AGCCCCGTGA GACAGGAGGA GGTGCAGTTC CTGGGGCTAA TGCTCGGCAC CACAGAAGCA 
CAGACAGCTT TGTACGTTCT CTTTTCTCTT GTGCTTCTTG GAGTCTGTGG CTTGGCATTT 
CTGTCATACA AGTACTGCAA GCGCTCTAAG CAAGGGAAAA CACAACGAAG CCAACAGGAA 
TTGAGCCCGG TGTCTTCATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_002290.2

Translation

MELSWHVVFIALLSFSCWGSDWESDRNFISTAGPLTNDLLHNLSGLLGDQSSNFVAGDKDMYVCHQPLPTFLPEYFSSLHASQITHYKVFLSWAQLLPAGSTQNPDEKTVQCYRRLLKALKTARLQPMVILHHQTLPASTLRRTEAFADLFADYATFAFHSFGDLVGIWFTFSDLEEVIKELPHQESRASQLQTLSDAHRKAYEIYHESYAFQGGKLSVVLRAEDIPELLLEPPISALAQDTVDFLSLDLSYECQNEASLRQKLSKLQTIEPKVKVFIFNLKLPDCPSTMKNPASLLFSLFEAINKDQVLTIGFDINEFLSCSSSSKKSMSCSLTGSLALQPDQQQDHETTDSSPASAYQRIWEAFANQSRAERDAFLQDTFPEGFLWGASTGAFNVEGGWAEGGRGVSIWDPRRPLNTTEGQATLEVASDSYHKVASDVALLCGLRAQVYKFSISWSRIFPMGHGSSPSLPGVAYYNKLIDRLQDAGIEPMATLFHWDLPQALQDHGGWQNESVVDAFLDYAAFCFSTFGDRVKLWVTFHEPWVMSYAGYGTGQHPPGISDPGVASFKVAHLVLKAHARTWHHYNSHHRPQQQGHVGIVLNSDWAEPLSPERPEDLRASERFLHFMLGWFAHPVFVDGDYPATLRTQIQQMNRQCSHPVAQLPEFTEAEKQLLKGSADFLGLSHYTSRLISNAPQNTCIPSYDTIGGFSQHVNHVWPQTSSSWIRVVPWGIRRLLQFVSLEYTRGKVPIYLAGNGMPIGESENLFDDSLRVDYFNQYINEVLKAIKEDSVDVRSYIARSLIDGFEGPSGYSQRFGLHHVNFSDSSKSRTPRKSAYFFTSIIEKNGFLTKGAKRLLPPNTVNLPSKVRAFTFPSEVPSKAKVVWEKFSSQPKFERDLFYHGTFRDDFLWGVSSSAYQIEGAWDADGKGPSIWDNFTHTPGSNVKDNATGDIACDSYHQLDADLNMLRALKVKAYRFSISWSRIFPTGRNSSINSHGVDYYNRLINGLVASNIFPMVTLFHWDLPQALQDIGGWENPALIDLFDSYADFCFQTFGDRVKFWMTFNEPMYLAWLGYGSGEFPPGVKDPGWAPYRIAHAVIKAHARVYHTYDEKYRQEQKGVISLSLSTHWAEPKSPGVPRDVEAADRMLQFSLGWFAHPIFRNGDYPDTMKWKVGNRSELQHLATSRLPSFTEEEKRFIRATADVFCLNTYYSRIVQHKTPRLNPPSYEDDQEMAEEEDPSWPSTAMNRAAPWGTRRLLNWIKEEYGDIPIYITENGVGLTNPNTEDTDRIFYHKTYINEALKAYRLDGIDLRGYVAWSLMDNFEWLNGYTVKFGLYHVDFNNTNRPRTARASARYYTEVITNNGMPLAREDEFLYGRFPEGFIWSAASAAYQIEGAWRADGKGLSIWDTFSHTPLRVENDAIGDVACDSYHKIAEDLVTLQNLGVSHYRFSISWSRILPDGTTRYINEAGLNYYVRLIDTLLAASIQPQVTIYHWDLPQTLQDVGGWENETIVQRFKEYADVLFQRLGDKVKFWITLNEPFVIAYQGYGYGTAAPGVSNRPGTAPYIVGHNLIKAHAEAWHLYNDVYRASQGGVISITISSDWAEPRDPSNQEDVEAARRYVQFMGGWFAHPIFKNGDYNEVMKTRIRDRSLAAGLNKSRLPEFTESEKRRINGTYDFFGFNHYTTVLAYNLNYATAISSFDADRGVASIADRSWPDSGSFWLKMTPFGFRRILNWLKEEYNDPPIYVTENGVSQREETDLNDTARIYYLRTYINEALKAVQDKVDLRGYTVWSAMDNFEWATGFSERFGLHFVNYSDPSLPRIPKASAKFYASVVRCNGFPDPATGPHACLHQPDAGPTISPVRQEEVQFLGLMLGTTEAQTALYVLFSLVLLGVCGLAFLSYKYCKRSKQGKTQRSQQELSPVSSF

Target ORF information:

RefSeq Version	NM_002299.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens lactase (LCT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002299.2

ORF Insert Sequence:

ATGGAGCTGT CTTGGCATGT AGTCTTTATT GCCCTGCTAA GTTTTTCATG CTGGGGGTCA 
GACTGGGAGT CTGATAGAAA TTTCATTTCC ACCGCTGGTC CTCTAACCAA TGACTTGCTG 
CACAACCTGA GTGGTCTCCT GGGAGACCAG AGTTCTAACT TTGTAGCAGG GGACAAAGAC 
ATGTATGTTT GTCACCAGCC ACTGCCCACT TTCCTGCCAG AATACTTCAG CAGTCTCCAT 
GCCAGTCAGA TCACCCATTA TAAGGTATTT CTGTCATGGG CACAGCTCCT CCCAGCAGGA 
AGCACCCAGA ATCCAGACGA GAAAACAGTG CAGTGCTACC GGCGACTCCT CAAGGCCCTC 
AAGACTGCAC GGCTTCAGCC CATGGTCATC CTGCACCACC AGACCCTCCC TGCCAGCACC 
CTCCGGAGAA CCGAAGCCTT TGCTGACCTC TTCGCCGACT ATGCCACATT CGCCTTCCAC 
TCCTTCGGGG ACCTAGTTGG GATCTGGTTC ACCTTCAGTG ACTTGGAGGA AGTGATCAAG 
GAGCTTCCCC ACCAGGAATC AAGAGCGTCA CAACTCCAGA CCCTCAGTGA TGCCCACAGA 
AAAGCCTATG AGATTTACCA CGAAAGCTAT GCTTTTCAGG GCGGAAAACT CTCTGTTGTC 
CTGCGAGCTG AAGATATCCC GGAGCTCCTG CTAGAACCAC CCATATCTGC GCTTGCCCAG 
GACACGGTCG ATTTCCTCTC TCTTGATTTG TCTTATGAAT GCCAAAATGA GGCAAGTCTG 
CGGCAGAAGC TGAGTAAATT GCAGACCATT GAGCCAAAAG TGAAAGTTTT CATCTTCAAC 
CTAAAACTCC CAGACTGCCC CTCCACCATG AAGAACCCAG CCAGTCTGCT CTTCAGCCTT 
TTTGAAGCCA TAAATAAAGA CCAAGTGCTC ACCATTGGGT TTGATATTAA TGAGTTTCTG 
AGTTGTTCAT CAAGTTCCAA GAAAAGCATG TCTTGTTCTC TGACTGGCAG CCTGGCCCTT 
CAGCCTGACC AGCAGCAGGA CCACGAGACC ACGGACTCCT CTCCTGCCTC TGCCTATCAG 
AGAATCTGGG AAGCATTTGC CAATCAGTCC AGGGCGGAAA GGGATGCCTT CCTGCAGGAT 
ACTTTCCCTG AAGGCTTCCT CTGGGGTGCC TCCACAGGAG CCTTTAACGT GGAAGGAGGC 
TGGGCCGAGG GTGGGAGAGG GGTGAGCATC TGGGATCCAC GCAGGCCCCT GAACACCACT 
GAGGGCCAAG CGACGCTGGA GGTGGCCAGC GACAGTTACC ACAAGGTAGC CTCTGACGTC 
GCCCTGCTTT GCGGCCTCCG GGCTCAGGTG TACAAGTTCT CCATCTCCTG GTCCCGGATC 
TTCCCCATGG GGCACGGGAG CAGCCCCAGC CTCCCAGGCG TTGCCTACTA CAACAAGCTG 
ATTGACAGGC TACAGGATGC GGGCATCGAG CCCATGGCCA CGCTGTTCCA CTGGGACCTG 
CCTCAGGCCC TGCAGGATCA TGGTGGATGG CAGAATGAGA GCGTGGTGGA TGCCTTCCTG 
GACTATGCGG CCTTCTGCTT CTCCACATTT GGGGACCGTG TGAAGCTGTG GGTGACCTTC 
CATGAGCCGT GGGTGATGAG CTACGCAGGC TATGGCACCG GCCAGCACCC TCCCGGCATC 
TCTGACCCAG GAGTGGCCTC TTTTAAGGTG GCTCACTTGG TCCTCAAGGC TCATGCCAGA 
ACTTGGCACC ACTACAACAG CCATCATCGC CCACAGCAGC AGGGGCACGT GGGCATTGTG 
CTGAACTCAG ACTGGGCAGA ACCCCTGTCT CCAGAGAGGC CTGAGGACCT GAGAGCCTCT 
GAGCGCTTCT TGCACTTCAT GCTGGGCTGG TTTGCACACC CCGTCTTTGT GGATGGAGAC 
TACCCAGCCA CCCTGAGGAC CCAGATCCAA CAGATGAACA GACAGTGCTC CCATCCTGTG 
GCTCAACTCC CCGAGTTCAC AGAGGCAGAG AAGCAGCTCC TGAAAGGCTC TGCTGATTTT 
CTGGGTCTGT CGCATTACAC CTCCCGCCTC ATCAGCAACG CCCCACAAAA CACCTGCATC 
CCTAGCTATG ATACCATTGG AGGCTTCTCC CAACACGTGA ACCATGTGTG GCCCCAGACC 
TCATCCTCTT GGATTCGTGT GGTGCCCTGG GGGATAAGGA GGCTGTTGCA GTTTGTATCC 
CTGGAATACA CAAGAGGAAA AGTTCCAATA TACCTTGCCG GGAATGGCAT GCCCATAGGG 
GAAAGTGAAA ATCTCTTTGA TGATTCCTTA AGAGTAGACT ACTTCAATCA ATATATCAAT 
GAGGTGCTCA AGGCTATCAA GGAAGACTCT GTGGATGTTC GTTCCTACAT TGCTCGTTCC 
CTCATTGATG GCTTCGAAGG CCCTTCTGGT TACAGCCAGC GGTTTGGCCT GCACCACGTC 
AACTTCAGCG ACAGCAGCAA GTCAAGGACT CCCAGGAAAT CTGCCTACTT TTTCACTAGC 
ATCATAGAAA AGAACGGTTT CCTCACCAAG GGGGCAAAAA GACTGCTACC ACCTAATACA 
GTAAACCTCC CCTCCAAAGT CAGAGCCTTC ACTTTTCCAT CTGAGGTGCC CTCCAAGGCT 
AAAGTCGTTT GGGAAAAGTT CTCCAGCCAA CCCAAGTTCG AAAGAGATTT GTTCTACCAC 
GGGACGTTTC GGGATGACTT TCTGTGGGGC GTGTCCTCTT CCGCTTATCA GATTGAAGGC 
GCGTGGGATG CCGATGGCAA AGGCCCCAGC ATCTGGGATA ACTTTACCCA CACACCAGGG 
AGCAATGTGA AAGACAATGC CACTGGAGAC ATCGCCTGTG ACAGCTATCA CCAGCTGGAT 
GCCGATCTGA ATATGCTCCG AGCTTTGAAG GTGAAGGCCT ACCGCTTCTC TATCTCCTGG 
TCTCGGATTT TCCCAACTGG GAGAAACAGC TCTATCAACA GTCATGGGGT TGATTATTAC 
AACAGGCTGA TCAATGGCTT GGTGGCAAGC AACATCTTTC CCATGGTGAC ATTGTTCCAT 
TGGGACCTGC CCCAGGCCCT CCAGGATATC GGAGGCTGGG AGAATCCTGC CTTGATTGAC 
TTGTTTGACA GCTACGCAGA CTTTTGTTTC CAGACCTTTG GTGATAGAGT CAAGTTTTGG 
ATGACTTTTA ATGAGCCCAT GTACCTGGCA TGGCTAGGTT ATGGCTCAGG GGAATTTCCC 
CCAGGGGTGA AGGACCCAGG CTGGGCACCA TATAGGATAG CCCACGCCGT CATCAAAGCC 
CATGCCAGAG TCTATCACAC GTACGATGAG AAATACAGGC AGGAGCAGAA GGGGGTCATC 
TCGCTGAGCC TCAGTACACA CTGGGCAGAG CCCAAGTCAC CAGGGGTCCC CAGAGATGTG 
GAAGCCGCTG ACCGAATGCT GCAGTTCTCC CTGGGCTGGT TTGCTCACCC CATTTTTAGA 
AACGGAGACT ATCCTGACAC CATGAAGTGG AAAGTGGGGA ACAGGAGTGA ACTGCAGCAC 
TTAGCCACCT CCCGCCTGCC AAGCTTCACT GAGGAAGAGA AGAGGTTCAT CAGGGCGACG 
GCCGACGTCT TCTGCCTCAA CACGTACTAC TCCAGAATCG TGCAGCACAA AACACCCAGG 
CTAAACCCAC CCTCCTACGA AGACGACCAG GAGATGGCTG AGGAGGAGGA CCCTTCGTGG 
CCTTCCACGG CAATGAACAG AGCTGCGCCC TGGGGGACGC GAAGGCTGCT GAACTGGATC 
AAGGAAGAGT ATGGTGACAT CCCCATTTAC ATCACCGAAA ACGGAGTGGG GCTGACCAAT 
CCGAACACGG AGGATACTGA TAGGATATTT TACCACAAAA CCTACATCAA TGAGGCTTTG 
AAAGCCTACA GGCTCGATGG TATAGACCTT CGAGGGTATG TCGCCTGGTC TCTGATGGAC 
AACTTTGAGT GGCTAAATGG CTACACGGTC AAGTTTGGAC TGTACCATGT TGATTTCAAC 
AACACGAACA GGCCTCGCAC AGCAAGAGCC TCCGCCAGGT ACTACACAGA GGTCATTACC 
AACAACGGCA TGCCACTGGC CAGGGAGGAT GAGTTTCTGT ACGGACGGTT TCCTGAGGGC 
TTCATCTGGA GTGCAGCTTC TGCTGCATAT CAGATTGAAG GTGCGTGGAG AGCAGATGGC 
AAAGGACTCA GCATTTGGGA CACGTTTTCT CACACACCAC TGAGGGTTGA GAACGATGCC 
ATTGGAGACG TGGCCTGTGA CAGTTATCAC AAGATTGCTG AGGATCTGGT CACCCTGCAG 
AACCTGGGCG TGTCCCACTA CCGTTTTTCC ATCTCCTGGT CTCGCATCCT CCCTGATGGA 
ACCACCAGGT ACATCAATGA AGCGGGCCTG AACTACTACG TGAGGCTCAT CGATACACTG 
CTGGCCGCCA GCATCCAGCC CCAGGTGACC ATTTACCACT GGGACCTACC ACAGACGCTC 
CAAGATGTAG GAGGCTGGGA GAATGAGACC ATCGTGCAGC GGTTTAAGGA GTATGCAGAT 
GTGCTCTTCC AGAGGCTGGG AGACAAGGTG AAGTTTTGGA TCACGCTGAA TGAGCCCTTT 
GTCATTGCTT ACCAGGGCTA TGGCTACGGA ACAGCAGCTC CAGGAGTCTC CAATAGGCCT 
GGCACTGCCC CCTACATTGT TGGCCACAAT CTAATAAAGG CTCATGCTGA GGCCTGGCAT 
CTGTACAACG ATGTGTACCG CGCCAGTCAA GGTGGCGTGA TTTCCATCAC CATCAGCAGT 
GACTGGGCTG AACCCAGAGA TCCCTCTAAC CAGGAGGATG TGGAGGCAGC CAGGAGATAT 
GTTCAGTTCA TGGGAGGCTG GTTTGCACAT CCTATTTTCA AGAATGGAGA TTACAATGAG 
GTGATGAAGA CGCGGATCCG TGACAGGAGC TTGGCTGCAG GCCTCAACAA GTCTCGGCTG 
CCAGAATTTA CAGAGAGTGA GAAGAGGAGG ATCAACGGCA CCTATGACTT TTTTGGGTTC 
AATCACTACA CCACTGTCCT CGCCTACAAC CTCAACTATG CCACTGCCAT CTCTTCTTTT 
GATGCAGACA GAGGAGTTGC TTCCATCGCA GATCGCTCGT GGCCAGACTC TGGCTCCTTC 
TGGCTGAAGA TGACGCCTTT TGGCTTCAGG AGGATCCTGA ACTGGTTAAA GGAGGAATAC 
AATGACCCTC CAATTTATGT CACAGAGAAT GGAGTGTCCC AGCGGGAAGA AACAGACCTC 
AATGACACTG CAAGGATCTA CTACCTTCGG ACTTACATCA ATGAGGCCCT CAAAGCTGTG 
CAGGACAAGG TGGACCTTCG AGGATACACA GTTTGGAGTG CGATGGACAA TTTTGAGTGG 
GCCACAGGCT TTTCAGAGAG ATTTGGTCTG CATTTTGTGA ACTACAGTGA CCCTTCTCTG 
CCAAGGATCC CCAAAGCATC AGCGAAGTTC TACGCCTCTG TGGTCCGATG CAATGGCTTC 
CCTGACCCCG CTACAGGGCC TCACGCTTGT CTCCACCAGC CAGATGCTGG ACCCACCATC 
AGCCCCGTGA GACAGGAGGA GGTGCAGTTC CTGGGGCTAA TGCTCGGCAC CACAGAAGCA 
CAGACAGCTT TGTACGTTCT CTTTTCTCTT GTGCTTCTTG GAGTCTGTGG CTTGGCATTT 
CTGTCATACA AGTACTGCAA GCGCTCTAAG CAAGGGAAAA CACAACGAAG CCAACAGGAA 
TTGAGCCCGG TGTCTTCATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LCT ( NM_002299.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002290.2 Homo sapiens lactase (LCT), mRNA.

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CloneID	OHu18202
Clone ID Related Accession (Same CDS sequence)	NM_002299.4 , NM_002299.2 , NM_002299.3
Accession Version	NM_002299.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Homo sapiens(human)
Product	lactase-phlorizin hydrolase preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X07994.1, AC011893.7 and BX642122.1. On Jan 15, 2016 this sequence version replaced NM_002299.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X07994.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGT CTTGGCATGT AGTCTTTATT GCCCTGCTAA GTTTTTCATG CTGGGGGTCA 
GACTGGGAGT CTGATAGAAA TTTCATTTCC ACCGCTGGTC CTCTAACCAA TGACTTGCTG 
CACAACCTGA GTGGTCTCCT GGGAGACCAG AGTTCTAACT TTGTAGCAGG GGACAAAGAC 
ATGTATGTTT GTCACCAGCC ACTGCCCACT TTCCTGCCAG AATACTTCAG CAGTCTCCAT 
GCCAGTCAGA TCACCCATTA TAAGGTATTT CTGTCATGGG CACAGCTCCT CCCAGCAGGA 
AGCACCCAGA ATCCAGACGA GAAAACAGTG CAGTGCTACC GGCGACTCCT CAAGGCCCTC 
AAGACTGCAC GGCTTCAGCC CATGGTCATC CTGCACCACC AGACCCTCCC TGCCAGCACC 
CTCCGGAGAA CCGAAGCCTT TGCTGACCTC TTCGCCGACT ATGCCACATT CGCCTTCCAC 
TCCTTCGGGG ACCTAGTTGG GATCTGGTTC ACCTTCAGTG ACTTGGAGGA AGTGATCAAG 
GAGCTTCCCC ACCAGGAATC AAGAGCGTCA CAACTCCAGA CCCTCAGTGA TGCCCACAGA 
AAAGCCTATG AGATTTACCA CGAAAGCTAT GCTTTTCAGG GCGGAAAACT CTCTGTTGTC 
CTGCGAGCTG AAGATATCCC GGAGCTCCTG CTAGAACCAC CCATATCTGC GCTTGCCCAG 
GACACGGTCG ATTTCCTCTC TCTTGATTTG TCTTATGAAT GCCAAAATGA GGCAAGTCTG 
CGGCAGAAGC TGAGTAAATT GCAGACCATT GAGCCAAAAG TGAAAGTTTT CATCTTCAAC 
CTAAAACTCC CAGACTGCCC CTCCACCATG AAGAACCCAG CCAGTCTGCT CTTCAGCCTT 
TTTGAAGCCA TAAATAAAGA CCAAGTGCTC ACCATTGGGT TTGATATTAA TGAGTTTCTG 
AGTTGTTCAT CAAGTTCCAA GAAAAGCATG TCTTGTTCTC TGACTGGCAG CCTGGCCCTT 
CAGCCTGACC AGCAGCAGGA CCACGAGACC ACGGACTCCT CTCCTGCCTC TGCCTATCAG 
AGAATCTGGG AAGCATTTGC CAATCAGTCC AGGGCGGAAA GGGATGCCTT CCTGCAGGAT 
ACTTTCCCTG AAGGCTTCCT CTGGGGTGCC TCCACAGGAG CCTTTAACGT GGAAGGAGGC 
TGGGCCGAGG GTGGGAGAGG GGTGAGCATC TGGGATCCAC GCAGGCCCCT GAACACCACT 
GAGGGCCAAG CGACGCTGGA GGTGGCCAGC GACAGTTACC ACAAGGTAGC CTCTGACGTC 
GCCCTGCTTT GCGGCCTCCG GGCTCAGGTG TACAAGTTCT CCATCTCCTG GTCCCGGATC 
TTCCCCATGG GGCACGGGAG CAGCCCCAGC CTCCCAGGCG TTGCCTACTA CAACAAGCTG 
ATTGACAGGC TACAGGATGC GGGCATCGAG CCCATGGCCA CGCTGTTCCA CTGGGACCTG 
CCTCAGGCCC TGCAGGATCA TGGTGGATGG CAGAATGAGA GCGTGGTGGA TGCCTTCCTG 
GACTATGCGG CCTTCTGCTT CTCCACATTT GGGGACCGTG TGAAGCTGTG GGTGACCTTC 
CATGAGCCGT GGGTGATGAG CTACGCAGGC TATGGCACCG GCCAGCACCC TCCCGGCATC 
TCTGACCCAG GAGTGGCCTC TTTTAAGGTG GCTCACTTGG TCCTCAAGGC TCATGCCAGA 
ACTTGGCACC ACTACAACAG CCATCATCGC CCACAGCAGC AGGGGCACGT GGGCATTGTG 
CTGAACTCAG ACTGGGCAGA ACCCCTGTCT CCAGAGAGGC CTGAGGACCT GAGAGCCTCT 
GAGCGCTTCT TGCACTTCAT GCTGGGCTGG TTTGCACACC CCGTCTTTGT GGATGGAGAC 
TACCCAGCCA CCCTGAGGAC CCAGATCCAA CAGATGAACA GACAGTGCTC CCATCCTGTG 
GCTCAACTCC CCGAGTTCAC AGAGGCAGAG AAGCAGCTCC TGAAAGGCTC TGCTGATTTT 
CTGGGTCTGT CGCATTACAC CTCCCGCCTC ATCAGCAACG CCCCACAAAA CACCTGCATC 
CCTAGCTATG ATACCATTGG AGGCTTCTCC CAACACGTGA ACCATGTGTG GCCCCAGACC 
TCATCCTCTT GGATTCGTGT GGTGCCCTGG GGGATAAGGA GGCTGTTGCA GTTTGTATCC 
CTGGAATACA CAAGAGGAAA AGTTCCAATA TACCTTGCCG GGAATGGCAT GCCCATAGGG 
GAAAGTGAAA ATCTCTTTGA TGATTCCTTA AGAGTAGACT ACTTCAATCA ATATATCAAT 
GAGGTGCTCA AGGCTATCAA GGAAGACTCT GTGGATGTTC GTTCCTACAT TGCTCGTTCC 
CTCATTGATG GCTTCGAAGG CCCTTCTGGT TACAGCCAGC GGTTTGGCCT GCACCACGTC 
AACTTCAGCG ACAGCAGCAA GTCAAGGACT CCCAGGAAAT CTGCCTACTT TTTCACTAGC 
ATCATAGAAA AGAACGGTTT CCTCACCAAG GGGGCAAAAA GACTGCTACC ACCTAATACA 
GTAAACCTCC CCTCCAAAGT CAGAGCCTTC ACTTTTCCAT CTGAGGTGCC CTCCAAGGCT 
AAAGTCGTTT GGGAAAAGTT CTCCAGCCAA CCCAAGTTCG AAAGAGATTT GTTCTACCAC 
GGGACGTTTC GGGATGACTT TCTGTGGGGC GTGTCCTCTT CCGCTTATCA GATTGAAGGC 
GCGTGGGATG CCGATGGCAA AGGCCCCAGC ATCTGGGATA ACTTTACCCA CACACCAGGG 
AGCAATGTGA AAGACAATGC CACTGGAGAC ATCGCCTGTG ACAGCTATCA CCAGCTGGAT 
GCCGATCTGA ATATGCTCCG AGCTTTGAAG GTGAAGGCCT ACCGCTTCTC TATCTCCTGG 
TCTCGGATTT TCCCAACTGG GAGAAACAGC TCTATCAACA GTCATGGGGT TGATTATTAC 
AACAGGCTGA TCAATGGCTT GGTGGCAAGC AACATCTTTC CCATGGTGAC ATTGTTCCAT 
TGGGACCTGC CCCAGGCCCT CCAGGATATC GGAGGCTGGG AGAATCCTGC CTTGATTGAC 
TTGTTTGACA GCTACGCAGA CTTTTGTTTC CAGACCTTTG GTGATAGAGT CAAGTTTTGG 
ATGACTTTTA ATGAGCCCAT GTACCTGGCA TGGCTAGGTT ATGGCTCAGG GGAATTTCCC 
CCAGGGGTGA AGGACCCAGG CTGGGCACCA TATAGGATAG CCCACGCCGT CATCAAAGCC 
CATGCCAGAG TCTATCACAC GTACGATGAG AAATACAGGC AGGAGCAGAA GGGGGTCATC 
TCGCTGAGCC TCAGTACACA CTGGGCAGAG CCCAAGTCAC CAGGGGTCCC CAGAGATGTG 
GAAGCCGCTG ACCGAATGCT GCAGTTCTCC CTGGGCTGGT TTGCTCACCC CATTTTTAGA 
AACGGAGACT ATCCTGACAC CATGAAGTGG AAAGTGGGGA ACAGGAGTGA ACTGCAGCAC 
TTAGCCACCT CCCGCCTGCC AAGCTTCACT GAGGAAGAGA AGAGGTTCAT CAGGGCGACG 
GCCGACGTCT TCTGCCTCAA CACGTACTAC TCCAGAATCG TGCAGCACAA AACACCCAGG 
CTAAACCCAC CCTCCTACGA AGACGACCAG GAGATGGCTG AGGAGGAGGA CCCTTCGTGG 
CCTTCCACGG CAATGAACAG AGCTGCGCCC TGGGGGACGC GAAGGCTGCT GAACTGGATC 
AAGGAAGAGT ATGGTGACAT CCCCATTTAC ATCACCGAAA ACGGAGTGGG GCTGACCAAT 
CCGAACACGG AGGATACTGA TAGGATATTT TACCACAAAA CCTACATCAA TGAGGCTTTG 
AAAGCCTACA GGCTCGATGG TATAGACCTT CGAGGGTATG TCGCCTGGTC TCTGATGGAC 
AACTTTGAGT GGCTAAATGG CTACACGGTC AAGTTTGGAC TGTACCATGT TGATTTCAAC 
AACACGAACA GGCCTCGCAC AGCAAGAGCC TCCGCCAGGT ACTACACAGA GGTCATTACC 
AACAACGGCA TGCCACTGGC CAGGGAGGAT GAGTTTCTGT ACGGACGGTT TCCTGAGGGC 
TTCATCTGGA GTGCAGCTTC TGCTGCATAT CAGATTGAAG GTGCGTGGAG AGCAGATGGC 
AAAGGACTCA GCATTTGGGA CACGTTTTCT CACACACCAC TGAGGGTTGA GAACGATGCC 
ATTGGAGACG TGGCCTGTGA CAGTTATCAC AAGATTGCTG AGGATCTGGT CACCCTGCAG 
AACCTGGGCG TGTCCCACTA CCGTTTTTCC ATCTCCTGGT CTCGCATCCT CCCTGATGGA 
ACCACCAGGT ACATCAATGA AGCGGGCCTG AACTACTACG TGAGGCTCAT CGATACACTG 
CTGGCCGCCA GCATCCAGCC CCAGGTGACC ATTTACCACT GGGACCTACC ACAGACGCTC 
CAAGATGTAG GAGGCTGGGA GAATGAGACC ATCGTGCAGC GGTTTAAGGA GTATGCAGAT 
GTGCTCTTCC AGAGGCTGGG AGACAAGGTG AAGTTTTGGA TCACGCTGAA TGAGCCCTTT 
GTCATTGCTT ACCAGGGCTA TGGCTACGGA ACAGCAGCTC CAGGAGTCTC CAATAGGCCT 
GGCACTGCCC CCTACATTGT TGGCCACAAT CTAATAAAGG CTCATGCTGA GGCCTGGCAT 
CTGTACAACG ATGTGTACCG CGCCAGTCAA GGTGGCGTGA TTTCCATCAC CATCAGCAGT 
GACTGGGCTG AACCCAGAGA TCCCTCTAAC CAGGAGGATG TGGAGGCAGC CAGGAGATAT 
GTTCAGTTCA TGGGAGGCTG GTTTGCACAT CCTATTTTCA AGAATGGAGA TTACAATGAG 
GTGATGAAGA CGCGGATCCG TGACAGGAGC TTGGCTGCAG GCCTCAACAA GTCTCGGCTG 
CCAGAATTTA CAGAGAGTGA GAAGAGGAGG ATCAACGGCA CCTATGACTT TTTTGGGTTC 
AATCACTACA CCACTGTCCT CGCCTACAAC CTCAACTATG CCACTGCCAT CTCTTCTTTT 
GATGCAGACA GAGGAGTTGC TTCCATCGCA GATCGCTCGT GGCCAGACTC TGGCTCCTTC 
TGGCTGAAGA TGACGCCTTT TGGCTTCAGG AGGATCCTGA ACTGGTTAAA GGAGGAATAC 
AATGACCCTC CAATTTATGT CACAGAGAAT GGAGTGTCCC AGCGGGAAGA AACAGACCTC 
AATGACACTG CAAGGATCTA CTACCTTCGG ACTTACATCA ATGAGGCCCT CAAAGCTGTG 
CAGGACAAGG TGGACCTTCG AGGATACACA GTTTGGAGTG CGATGGACAA TTTTGAGTGG 
GCCACAGGCT TTTCAGAGAG ATTTGGTCTG CATTTTGTGA ACTACAGTGA CCCTTCTCTG 
CCAAGGATCC CCAAAGCATC AGCGAAGTTC TACGCCTCTG TGGTCCGATG CAATGGCTTC 
CCTGACCCCG CTACAGGGCC TCACGCTTGT CTCCACCAGC CAGATGCTGG ACCCACCATC 
AGCCCCGTGA GACAGGAGGA GGTGCAGTTC CTGGGGCTAA TGCTCGGCAC CACAGAAGCA 
CAGACAGCTT TGTACGTTCT CTTTTCTCTT GTGCTTCTTG GAGTCTGTGG CTTGGCATTT 
CTGTCATACA AGTACTGCAA GCGCTCTAAG CAAGGGAAAA CACAACGAAG CCAACAGGAA 
TTGAGCCCGG TGTCTTCATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002290.2
CDS	12..5795
Translation	MELSWHVVFIALLSFSCWGSDWESDRNFISTAGPLTNDLLHNLSGLLGDQSSNFVAGDKDMYVCHQPLPTFLPEYFSSLHASQITHYKVFLSWAQLLPAGSTQNPDEKTVQCYRRLLKALKTARLQPMVILHHQTLPASTLRRTEAFADLFADYATFAFHSFGDLVGIWFTFSDLEEVIKELPHQESRASQLQTLSDAHRKAYEIYHESYAFQGGKLSVVLRAEDIPELLLEPPISALAQDTVDFLSLDLSYECQNEASLRQKLSKLQTIEPKVKVFIFNLKLPDCPSTMKNPASLLFSLFEAINKDQVLTIGFDINEFLSCSSSSKKSMSCSLTGSLALQPDQQQDHETTDSSPASAYQRIWEAFANQSRAERDAFLQDTFPEGFLWGASTGAFNVEGGWAEGGRGVSIWDPRRPLNTTEGQATLEVASDSYHKVASDVALLCGLRAQVYKFSISWSRIFPMGHGSSPSLPGVAYYNKLIDRLQDAGIEPMATLFHWDLPQALQDHGGWQNESVVDAFLDYAAFCFSTFGDRVKLWVTFHEPWVMSYAGYGTGQHPPGISDPGVASFKVAHLVLKAHARTWHHYNSHHRPQQQGHVGIVLNSDWAEPLSPERPEDLRASERFLHFMLGWFAHPVFVDGDYPATLRTQIQQMNRQCSHPVAQLPEFTEAEKQLLKGSADFLGLSHYTSRLISNAPQNTCIPSYDTIGGFSQHVNHVWPQTSSSWIRVVPWGIRRLLQFVSLEYTRGKVPIYLAGNGMPIGESENLFDDSLRVDYFNQYINEVLKAIKEDSVDVRSYIARSLIDGFEGPSGYSQRFGLHHVNFSDSSKSRTPRKSAYFFTSIIEKNGFLTKGAKRLLPPNTVNLPSKVRAFTFPSEVPSKAKVVWEKFSSQPKFERDLFYHGTFRDDFLWGVSSSAYQIEGAWDADGKGPSIWDNFTHTPGSNVKDNATGDIACDSYHQLDADLNMLRALKVKAYRFSISWSRIFPTGRNSSINSHGVDYYNRLINGLVASNIFPMVTLFHWDLPQALQDIGGWENPALIDLFDSYADFCFQTFGDRVKFWMTFNEPMYLAWLGYGSGEFPPGVKDPGWAPYRIAHAVIKAHARVYHTYDEKYRQEQKGVISLSLSTHWAEPKSPGVPRDVEAADRMLQFSLGWFAHPIFRNGDYPDTMKWKVGNRSELQHLATSRLPSFTEEEKRFIRATADVFCLNTYYSRIVQHKTPRLNPPSYEDDQEMAEEEDPSWPSTAMNRAAPWGTRRLLNWIKEEYGDIPIYITENGVGLTNPNTEDTDRIFYHKTYINEALKAYRLDGIDLRGYVAWSLMDNFEWLNGYTVKFGLYHVDFNNTNRPRTARASARYYTEVITNNGMPLAREDEFLYGRFPEGFIWSAASAAYQIEGAWRADGKGLSIWDTFSHTPLRVENDAIGDVACDSYHKIAEDLVTLQNLGVSHYRFSISWSRILPDGTTRYINEAGLNYYVRLIDTLLAASIQPQVTIYHWDLPQTLQDVGGWENETIVQRFKEYADVLFQRLGDKVKFWITLNEPFVIAYQGYGYGTAAPGVSNRPGTAPYIVGHNLIKAHAEAWHLYNDVYRASQGGVISITISSDWAEPRDPSNQEDVEAARRYVQFMGGWFAHPIFKNGDYNEVMKTRIRDRSLAAGLNKSRLPEFTESEKRRINGTYDFFGFNHYTTVLAYNLNYATAISSFDADRGVASIADRSWPDSGSFWLKMTPFGFRRILNWLKEEYNDPPIYVTENGVSQREETDLNDTARIYYLRTYINEALKAVQDKVDLRGYTVWSAMDNFEWATGFSERFGLHFVNYSDPSLPRIPKASAKFYASVVRCNGFPDPATGPHACLHQPDAGPTISPVRQEEVQFLGLMLGTTEAQTALYVLFSLVLLGVCGLAFLSYKYCKRSKQGKTQRSQQELSPVSSF

Target ORF information:

RefSeq Version	NM_002299.3
Organism	Homo sapiens(human)
Definition	Homo sapiens lactase (LCT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002299.3

ORF Insert Sequence:

ATGGAGCTGT CTTGGCATGT AGTCTTTATT GCCCTGCTAA GTTTTTCATG CTGGGGGTCA 
GACTGGGAGT CTGATAGAAA TTTCATTTCC ACCGCTGGTC CTCTAACCAA TGACTTGCTG 
CACAACCTGA GTGGTCTCCT GGGAGACCAG AGTTCTAACT TTGTAGCAGG GGACAAAGAC 
ATGTATGTTT GTCACCAGCC ACTGCCCACT TTCCTGCCAG AATACTTCAG CAGTCTCCAT 
GCCAGTCAGA TCACCCATTA TAAGGTATTT CTGTCATGGG CACAGCTCCT CCCAGCAGGA 
AGCACCCAGA ATCCAGACGA GAAAACAGTG CAGTGCTACC GGCGACTCCT CAAGGCCCTC 
AAGACTGCAC GGCTTCAGCC CATGGTCATC CTGCACCACC AGACCCTCCC TGCCAGCACC 
CTCCGGAGAA CCGAAGCCTT TGCTGACCTC TTCGCCGACT ATGCCACATT CGCCTTCCAC 
TCCTTCGGGG ACCTAGTTGG GATCTGGTTC ACCTTCAGTG ACTTGGAGGA AGTGATCAAG 
GAGCTTCCCC ACCAGGAATC AAGAGCGTCA CAACTCCAGA CCCTCAGTGA TGCCCACAGA 
AAAGCCTATG AGATTTACCA CGAAAGCTAT GCTTTTCAGG GCGGAAAACT CTCTGTTGTC 
CTGCGAGCTG AAGATATCCC GGAGCTCCTG CTAGAACCAC CCATATCTGC GCTTGCCCAG 
GACACGGTCG ATTTCCTCTC TCTTGATTTG TCTTATGAAT GCCAAAATGA GGCAAGTCTG 
CGGCAGAAGC TGAGTAAATT GCAGACCATT GAGCCAAAAG TGAAAGTTTT CATCTTCAAC 
CTAAAACTCC CAGACTGCCC CTCCACCATG AAGAACCCAG CCAGTCTGCT CTTCAGCCTT 
TTTGAAGCCA TAAATAAAGA CCAAGTGCTC ACCATTGGGT TTGATATTAA TGAGTTTCTG 
AGTTGTTCAT CAAGTTCCAA GAAAAGCATG TCTTGTTCTC TGACTGGCAG CCTGGCCCTT 
CAGCCTGACC AGCAGCAGGA CCACGAGACC ACGGACTCCT CTCCTGCCTC TGCCTATCAG 
AGAATCTGGG AAGCATTTGC CAATCAGTCC AGGGCGGAAA GGGATGCCTT CCTGCAGGAT 
ACTTTCCCTG AAGGCTTCCT CTGGGGTGCC TCCACAGGAG CCTTTAACGT GGAAGGAGGC 
TGGGCCGAGG GTGGGAGAGG GGTGAGCATC TGGGATCCAC GCAGGCCCCT GAACACCACT 
GAGGGCCAAG CGACGCTGGA GGTGGCCAGC GACAGTTACC ACAAGGTAGC CTCTGACGTC 
GCCCTGCTTT GCGGCCTCCG GGCTCAGGTG TACAAGTTCT CCATCTCCTG GTCCCGGATC 
TTCCCCATGG GGCACGGGAG CAGCCCCAGC CTCCCAGGCG TTGCCTACTA CAACAAGCTG 
ATTGACAGGC TACAGGATGC GGGCATCGAG CCCATGGCCA CGCTGTTCCA CTGGGACCTG 
CCTCAGGCCC TGCAGGATCA TGGTGGATGG CAGAATGAGA GCGTGGTGGA TGCCTTCCTG 
GACTATGCGG CCTTCTGCTT CTCCACATTT GGGGACCGTG TGAAGCTGTG GGTGACCTTC 
CATGAGCCGT GGGTGATGAG CTACGCAGGC TATGGCACCG GCCAGCACCC TCCCGGCATC 
TCTGACCCAG GAGTGGCCTC TTTTAAGGTG GCTCACTTGG TCCTCAAGGC TCATGCCAGA 
ACTTGGCACC ACTACAACAG CCATCATCGC CCACAGCAGC AGGGGCACGT GGGCATTGTG 
CTGAACTCAG ACTGGGCAGA ACCCCTGTCT CCAGAGAGGC CTGAGGACCT GAGAGCCTCT 
GAGCGCTTCT TGCACTTCAT GCTGGGCTGG TTTGCACACC CCGTCTTTGT GGATGGAGAC 
TACCCAGCCA CCCTGAGGAC CCAGATCCAA CAGATGAACA GACAGTGCTC CCATCCTGTG 
GCTCAACTCC CCGAGTTCAC AGAGGCAGAG AAGCAGCTCC TGAAAGGCTC TGCTGATTTT 
CTGGGTCTGT CGCATTACAC CTCCCGCCTC ATCAGCAACG CCCCACAAAA CACCTGCATC 
CCTAGCTATG ATACCATTGG AGGCTTCTCC CAACACGTGA ACCATGTGTG GCCCCAGACC 
TCATCCTCTT GGATTCGTGT GGTGCCCTGG GGGATAAGGA GGCTGTTGCA GTTTGTATCC 
CTGGAATACA CAAGAGGAAA AGTTCCAATA TACCTTGCCG GGAATGGCAT GCCCATAGGG 
GAAAGTGAAA ATCTCTTTGA TGATTCCTTA AGAGTAGACT ACTTCAATCA ATATATCAAT 
GAGGTGCTCA AGGCTATCAA GGAAGACTCT GTGGATGTTC GTTCCTACAT TGCTCGTTCC 
CTCATTGATG GCTTCGAAGG CCCTTCTGGT TACAGCCAGC GGTTTGGCCT GCACCACGTC 
AACTTCAGCG ACAGCAGCAA GTCAAGGACT CCCAGGAAAT CTGCCTACTT TTTCACTAGC 
ATCATAGAAA AGAACGGTTT CCTCACCAAG GGGGCAAAAA GACTGCTACC ACCTAATACA 
GTAAACCTCC CCTCCAAAGT CAGAGCCTTC ACTTTTCCAT CTGAGGTGCC CTCCAAGGCT 
AAAGTCGTTT GGGAAAAGTT CTCCAGCCAA CCCAAGTTCG AAAGAGATTT GTTCTACCAC 
GGGACGTTTC GGGATGACTT TCTGTGGGGC GTGTCCTCTT CCGCTTATCA GATTGAAGGC 
GCGTGGGATG CCGATGGCAA AGGCCCCAGC ATCTGGGATA ACTTTACCCA CACACCAGGG 
AGCAATGTGA AAGACAATGC CACTGGAGAC ATCGCCTGTG ACAGCTATCA CCAGCTGGAT 
GCCGATCTGA ATATGCTCCG AGCTTTGAAG GTGAAGGCCT ACCGCTTCTC TATCTCCTGG 
TCTCGGATTT TCCCAACTGG GAGAAACAGC TCTATCAACA GTCATGGGGT TGATTATTAC 
AACAGGCTGA TCAATGGCTT GGTGGCAAGC AACATCTTTC CCATGGTGAC ATTGTTCCAT 
TGGGACCTGC CCCAGGCCCT CCAGGATATC GGAGGCTGGG AGAATCCTGC CTTGATTGAC 
TTGTTTGACA GCTACGCAGA CTTTTGTTTC CAGACCTTTG GTGATAGAGT CAAGTTTTGG 
ATGACTTTTA ATGAGCCCAT GTACCTGGCA TGGCTAGGTT ATGGCTCAGG GGAATTTCCC 
CCAGGGGTGA AGGACCCAGG CTGGGCACCA TATAGGATAG CCCACGCCGT CATCAAAGCC 
CATGCCAGAG TCTATCACAC GTACGATGAG AAATACAGGC AGGAGCAGAA GGGGGTCATC 
TCGCTGAGCC TCAGTACACA CTGGGCAGAG CCCAAGTCAC CAGGGGTCCC CAGAGATGTG 
GAAGCCGCTG ACCGAATGCT GCAGTTCTCC CTGGGCTGGT TTGCTCACCC CATTTTTAGA 
AACGGAGACT ATCCTGACAC CATGAAGTGG AAAGTGGGGA ACAGGAGTGA ACTGCAGCAC 
TTAGCCACCT CCCGCCTGCC AAGCTTCACT GAGGAAGAGA AGAGGTTCAT CAGGGCGACG 
GCCGACGTCT TCTGCCTCAA CACGTACTAC TCCAGAATCG TGCAGCACAA AACACCCAGG 
CTAAACCCAC CCTCCTACGA AGACGACCAG GAGATGGCTG AGGAGGAGGA CCCTTCGTGG 
CCTTCCACGG CAATGAACAG AGCTGCGCCC TGGGGGACGC GAAGGCTGCT GAACTGGATC 
AAGGAAGAGT ATGGTGACAT CCCCATTTAC ATCACCGAAA ACGGAGTGGG GCTGACCAAT 
CCGAACACGG AGGATACTGA TAGGATATTT TACCACAAAA CCTACATCAA TGAGGCTTTG 
AAAGCCTACA GGCTCGATGG TATAGACCTT CGAGGGTATG TCGCCTGGTC TCTGATGGAC 
AACTTTGAGT GGCTAAATGG CTACACGGTC AAGTTTGGAC TGTACCATGT TGATTTCAAC 
AACACGAACA GGCCTCGCAC AGCAAGAGCC TCCGCCAGGT ACTACACAGA GGTCATTACC 
AACAACGGCA TGCCACTGGC CAGGGAGGAT GAGTTTCTGT ACGGACGGTT TCCTGAGGGC 
TTCATCTGGA GTGCAGCTTC TGCTGCATAT CAGATTGAAG GTGCGTGGAG AGCAGATGGC 
AAAGGACTCA GCATTTGGGA CACGTTTTCT CACACACCAC TGAGGGTTGA GAACGATGCC 
ATTGGAGACG TGGCCTGTGA CAGTTATCAC AAGATTGCTG AGGATCTGGT CACCCTGCAG 
AACCTGGGCG TGTCCCACTA CCGTTTTTCC ATCTCCTGGT CTCGCATCCT CCCTGATGGA 
ACCACCAGGT ACATCAATGA AGCGGGCCTG AACTACTACG TGAGGCTCAT CGATACACTG 
CTGGCCGCCA GCATCCAGCC CCAGGTGACC ATTTACCACT GGGACCTACC ACAGACGCTC 
CAAGATGTAG GAGGCTGGGA GAATGAGACC ATCGTGCAGC GGTTTAAGGA GTATGCAGAT 
GTGCTCTTCC AGAGGCTGGG AGACAAGGTG AAGTTTTGGA TCACGCTGAA TGAGCCCTTT 
GTCATTGCTT ACCAGGGCTA TGGCTACGGA ACAGCAGCTC CAGGAGTCTC CAATAGGCCT 
GGCACTGCCC CCTACATTGT TGGCCACAAT CTAATAAAGG CTCATGCTGA GGCCTGGCAT 
CTGTACAACG ATGTGTACCG CGCCAGTCAA GGTGGCGTGA TTTCCATCAC CATCAGCAGT 
GACTGGGCTG AACCCAGAGA TCCCTCTAAC CAGGAGGATG TGGAGGCAGC CAGGAGATAT 
GTTCAGTTCA TGGGAGGCTG GTTTGCACAT CCTATTTTCA AGAATGGAGA TTACAATGAG 
GTGATGAAGA CGCGGATCCG TGACAGGAGC TTGGCTGCAG GCCTCAACAA GTCTCGGCTG 
CCAGAATTTA CAGAGAGTGA GAAGAGGAGG ATCAACGGCA CCTATGACTT TTTTGGGTTC 
AATCACTACA CCACTGTCCT CGCCTACAAC CTCAACTATG CCACTGCCAT CTCTTCTTTT 
GATGCAGACA GAGGAGTTGC TTCCATCGCA GATCGCTCGT GGCCAGACTC TGGCTCCTTC 
TGGCTGAAGA TGACGCCTTT TGGCTTCAGG AGGATCCTGA ACTGGTTAAA GGAGGAATAC 
AATGACCCTC CAATTTATGT CACAGAGAAT GGAGTGTCCC AGCGGGAAGA AACAGACCTC 
AATGACACTG CAAGGATCTA CTACCTTCGG ACTTACATCA ATGAGGCCCT CAAAGCTGTG 
CAGGACAAGG TGGACCTTCG AGGATACACA GTTTGGAGTG CGATGGACAA TTTTGAGTGG 
GCCACAGGCT TTTCAGAGAG ATTTGGTCTG CATTTTGTGA ACTACAGTGA CCCTTCTCTG 
CCAAGGATCC CCAAAGCATC AGCGAAGTTC TACGCCTCTG TGGTCCGATG CAATGGCTTC 
CCTGACCCCG CTACAGGGCC TCACGCTTGT CTCCACCAGC CAGATGCTGG ACCCACCATC 
AGCCCCGTGA GACAGGAGGA GGTGCAGTTC CTGGGGCTAA TGCTCGGCAC CACAGAAGCA 
CAGACAGCTT TGTACGTTCT CTTTTCTCTT GTGCTTCTTG GAGTCTGTGG CTTGGCATTT 
CTGTCATACA AGTACTGCAA GCGCTCTAAG CAAGGGAAAA CACAACGAAG CCAACAGGAA 
TTGAGCCCGG TGTCTTCATT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LCT ( XM_017004088.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859577.1 Homo sapiens lactase (LCT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu89445
Clone ID Related Accession (Same CDS sequence)	XM_017004088.2 , XM_017004088.1
Accession Version	XM_017004088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lactase-phlorizin hydrolase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017004088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGT CTTGGCATGT AGTCTTTATT GCCCTGCTAA GTTTTTCATG CTGGGGGTCA 
GACTGGGAGT CTGATAGAAA TTTCATTTCC ACCGCTGGTC CTCTAACCAA TGACTTGCTG 
CACAACCTGA GTGGTCTCCT GGGAGACCAG AGTTCTAACT TTGTAGCAGG GGACAAAGAC 
ATGTATGTTT GTCACCAGCC ACTGCCCACT TTCCTGCCAG AATACTTCAG CAGTCTCCAT 
GCCAGTCAGA TCACCCATTA TAAGGTATTT CTGTCATGGG CACAGCTCCT CCCAGCAGGA 
AGCACCCAGA ATCCAGACGA GAAAACAGTG CAGTGCTACC GGCGACTCCT CAAGGCCCTC 
AAGACTGCAC GGCTTCAGCC CATGGTCATC CTGCACCACC AGACCCTCCC TGCCAGCACC 
CTCCGGAGAA CCGAAGCCTT TGCTGACCTC TTCGCCGACT ATGCCACATT CGCCTTCCAC 
TCCTTCGGGG ACCTAGTTGG GATCTGGTTC ACCTTCAGTG ACTTGGAGGA AGTGATCAAG 
GAGCTTCCCC ACCAGGAATC AAGAGCGTCA CAACTCCAGA CCCTCAGTGA TGCCCACAGA 
AAAGCCTATG AGATTTACCA CGAAAGCTAT GCTTTTCAGG GCGGAAAACT CTCTGTTGTC 
CTGCGAGCTG AAGATATCCC GGAGCTCCTG CTAGAACCAC CCATATCTGC GCTTGCCCAG 
GACACGGTCG ATTTCCTCTC TCTTGATTTG TCTTATGAAT GCCAAAATGA GGCAAGTCTG 
CGGCAGAAGC TGAGTAAATT GCAGACCATT GAGCCAAAAG TGAAAGTTTT CATCTTCAAC 
CTAAAACTCC CAGACTGCCC CTCCACCATG AAGAACCCAG CCAGTCTGCT CTTCAGCCTT 
TTTGAAGCCA TAAATAAAGA CCAAGTGCTC ACCATTGGGT TTGATATTAA TGAGTTTCTG 
AGTTGTTCAT CAAGTTCCAA GAAAAGCATG TCTTGTTCTC TGACTGGCAG CCTGGCCCTT 
CAGCCTGACC AGCAGCAGGA CCACGAGACC ACGGACTCCT CTCCTGCCTC TGCCTATCAG 
AGAATCTGGG AAGCATTTGC CAATCAGTCC AGGGCGGAAA GGGATGCCTT CCTGCAGGAT 
ACTTTCCCTG AAGGCTTCCT CTGGGGTGCC TCCACAGGAG CCTTTAACGT GGAAGGAGGC 
TGGGCCGAGG GTGGGAGAGG GGTGAGCATC TGGGATCCAC GCAGGCCCCT GAACACCACT 
GAGGGCCAAG CGACGCTGGA GGTGGCCAGC GACAGTTACC ACAAGGTAGC CTCTGACGTC 
GCCCTGCTTT GCGGCCTCCG GGCTCAGGTG TACAAGTTCT CCATCTCCTG GTCCCGGATC 
TTCCCCATGG GGCACGGGAG CAGCCCCAGC CTCCCAGGCG TTGCCTACTA CAACAAGCTG 
ATTGACAGGC TACAGGATGC GGGCATCGAG CCCATGGCCA CGCTGTTCCA CTGGGACCTG 
CCTCAGGCCC TGCAGGATCA TGGTGGATGG CAGAATGAGA GCGTGGTGGA TGCCTTCCTG 
GACTATGCGG CCTTCTGCTT CTCCACATTT GGGGACCGTG TGAAGCTGTG GGTGACCTTC 
CATGAGCCGT GGGTGATGAG CTACGCAGGC TATGGCACCG GCCAGCACCC TCCCGGCATC 
TCTGACCCAG GAGTGGCCTC TTTTAAGGTG GCTCACTTGG TCCTCAAGGC TCATGCCAGA 
ACTTGGCACC ACTACAACAG CCATCATCGC CCACAGCAGC AGGGGCACGT GGGCATTGTG 
CTGAACTCAG ACTGGGCAGA ACCCCTGTCT CCAGAGAGGC CTGAGGACCT GAGAGCCTCT 
GAGCGCTTCT TGCACTTCAT GCTGGGCTGG TTTGCACACC CCGTCTTTGT GGATGGAGAC 
TACCCAGCCA CCCTGAGGAC CCAGATCCAA CAGATGAACA GACAGTGCTC CCATCCTGTG 
GCTCAACTCC CCGAGTTCAC AGAGGCAGAG AAGCAGCTCC TGAAAGGCTC TGCTGATTTT 
CTGGGTCTGT CGCATTACAC CTCCCGCCTC ATCAGCAACG CCCCACAAAA CACCTGCATC 
CCTAGCTATG ATACCATTGG AGGCTTCTCC CAACACGTGA ACCATGTGTG GCCCCAGACC 
TCATCCTCTT GGATTCGTGT GGTGCCCTGG GGGATAAGGA GGCTGTTGCA GTTTGTATCC 
CTGGAATACA CAAGAGGAAA AGTTCCAATA TACCTTGCCG GGAATGGCAT GCCCATAGGG 
GAAAGTGAAA ATCTCTTTGA TGATTCCTTA AGAGTAGACT ACTTCAATCA ATATATCAAT 
GAGGTGCTCA AGGCTATCAA GGAAGACTCT GTGGATGTTC GTTCCTACAT TGCTCGTTCC 
CTCATTGATG GCTTCGAAGG CCCTTCTGGT TACAGCCAGC GGTTTGGCCT GCACCACGTC 
AACTTCAGCG ACAGCAGCAA GTCAAGGACT CCCAGGAAAT CTGCCTACTT TTTCACTAGC 
ATCATAGAAA AGAACGGTTT CCTCACCAAG GGGGCAAAAA GACTGCTACC ACCTAATACA 
GTAAACCTCC CCTCCAAAGT CAGAGCCTTC ACTTTTCCAT CTGAGGTGCC CTCCAAGGCT 
AAAGTCGTTT GGGAAAAGTT CTCCAGCCAA CCCAAGTTCG AAAGAGATTT GTTCTACCAC 
GGGACGTTTC GGGATGACTT TCTGTGGGGC GTGTCCTCTT CCGCTTATCA GATTGAAGGC 
GCGTGGGATG CCGATGGCAA AGGCCCCAGC ATCTGGGATA ACTTTACCCA CACACCAGGG 
AGCAATGTGA AAGACAATGC CACTGGAGAC ATCGCCTGTG ACAGCTATCA CCAGCTGGAT 
GCCGATCTGA ATATGCTCCG AGCTTTGAAG GTGAAGGCCT ACCGCTTCTC TATCTCCTGG 
TCTCGGATTT TCCCAACTGG GAGAAACAGC TCTATCAACA GTCATGGGGT TGATTATTAC 
AACAGGCTGA TCAATGGCTT GGTGGCAAGC AACATCTTTC CCATGGTGAC ATTGTTCCAT 
TGGGACCTGC CCCAGGCCCT CCAGGATATC GGAGGCTGGG AGAATCCTGC CTTGATTGAC 
TTGTTTGACA GCTACGCAGA CTTTTGTTTC CAGACCTTTG GTGATAGAGT CAAGTTTTGG 
ATGACTTTTA ATGAGCCCAT GTACCTGGCA TGGCTAGGTT ATGGCTCAGG GGAATTTCCC 
CCAGGGGTGA AGGACCCAGG CTGGGCACCA TATAGGATAG CCCACGCCGT CATCAAAGCC 
CATGCCAGAG TCTATCACAC GTACGATGAG AAATACAGGC AGGAGCAGAA GGGGGTCATC 
TCGCTGAGCC TCAGTACACA CTGGGCAGAG CCCAAGTCAC CAGGGGTCCC CAGAGATGTG 
GAAGCCGCTG ACCGAATGCT GCAGTTCTCC CTGGGCTGGT TTGCTCACCC CATTTTTAGA 
AACGGAGACT ATCCTGACAC CATGAAGTGG AAAGTGGGGA ACAGGAGTGA ACTGCAGCAC 
TTAGCCACCT CCCGCCTGCC AAGCTTCACT GAGGAAGAGA AGAGGTTCAT CAGGGCGACG 
GCCGACGTCT TCTGCCTCAA CACGTACTAC TCCAGAATCG TGCAGCACAA AACACCCAGG 
CTAAACCCAC CCTCCTACGA AGACGACCAG GAGATGGCTG AGGAGGAGGA CCCTTCGTGG 
CCTTCCACGG CAATGAACAG AGCTGCGCCC TGGGGGACGC GAAGGCTGCT GAACTGGATC 
AAGGAAGAGT ATGGTGACAT CCCCATTTAC ATCACCGAAA ACGGAGTGGG GCTGACCAAT 
CCGAACACGG AGGATACTGA TAGGATATTT TACCACAAAA CCTACATCAA TGAGGCTTTG 
AAAGCCTACA GGCTCGATGG TATAGACCTT CGAGGGTATG TCGCCTGGTC TCTGATGGAC 
AACTTTGAGT GGCTAAATGG CTACACGGTC AAGTTTGGAC TGTACCATGT TGATTTCAAC 
AACACGAACA GGCCTCGCAC AGCAAGAGCC TCCGCCAGGT ACTACACAGA GGTCATTACC 
AACAACGGCA TGCCACTGGC CAGGGAGGAT GAGTTTCTGT ACGGACGGTT TCCTGAGGGC 
TTCATCTGGA GTGCAGCTTC TGCTGCATAT CAGATTGAAG GTGCGTGGAG AGCAGATGGC 
AAAGGACTCA GCATTTGGGA CACGTTTTCT CACACACCAC TGAGGGTTGA GAACGATGCC 
ATTGGAGACG TGGCCTGTGA CAGTTATCAC AAGATTGCTG AGGATCTGGT CACCCTGCAG 
AACCTGGGCG TGTCCCACTA CCGTTTTTCC ATCTCCTGGT CTCGCATCCT CCCTGATGGA 
ACCACCAGGT ACATCAATGA AGCGGGCCTG AACTACTACG TGAGGCTCAT CGATACACTG 
CTGGCCGCCA GCATCCAGCC CCAGGTGACC ATTTACCACT GGGACCTACC ACAGACGCTC 
CAAGATGTAG GAGGCTGGGA GAATGAGACC ATCGTGCAGC GGTTTAAGGA GTATGCAGAT 
GTGCTCTTCC AGAGGCTGGG AGACAAGGTG AAGTTTTGGA TCACGCTGAA TGAGCCCTTT 
GTCATTGCTT ACCAGGGCTA TGGCTACGGA ACAGCAGCTC CAGGAGTCTC CAATAGGCCT 
GGCACTGCCC CCTACATTGT TGGCCACAAT CTAATAAAGG CTCATGCTGA GGCCTGGCAT 
CTGTACAACG ATGTGTACCG CGCCAGTCAA GGTGGCGTGA TTTCCATCAC CATCAGCAGT 
GACTGGGCTG AACCCAGAGA TCCCTCTAAC CAGGAGGATG TGGAGGCAGC CAGGAGATAT 
GTTCAGTTCA TGGGAGGCTG GTTTGCACAT CCTATTTTCA AGAATGGAGA TTACAATGAG 
GTGATGAAGA CGCGGATCCG TGACAGGAGC TTGGCTGCAG GCCTCAACAA GTCTCGGCTG 
CCAGAATTTA CAGAGAGTGA GAAGAGGAGG ATCAACGGCA CCTATGACTT TTTTGGGTTC 
AATCACTACA CCACTGTCCT CGCCTACAAC CTCAACTATG CCACTGCCAT CTCTTCTTTT 
GATGCAGACA GGGCCTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859577.1
CDS	27..5147
Translation	MELSWHVVFIALLSFSCWGSDWESDRNFISTAGPLTNDLLHNLSGLLGDQSSNFVAGDKDMYVCHQPLPTFLPEYFSSLHASQITHYKVFLSWAQLLPAGSTQNPDEKTVQCYRRLLKALKTARLQPMVILHHQTLPASTLRRTEAFADLFADYATFAFHSFGDLVGIWFTFSDLEEVIKELPHQESRASQLQTLSDAHRKAYEIYHESYAFQGGKLSVVLRAEDIPELLLEPPISALAQDTVDFLSLDLSYECQNEASLRQKLSKLQTIEPKVKVFIFNLKLPDCPSTMKNPASLLFSLFEAINKDQVLTIGFDINEFLSCSSSSKKSMSCSLTGSLALQPDQQQDHETTDSSPASAYQRIWEAFANQSRAERDAFLQDTFPEGFLWGASTGAFNVEGGWAEGGRGVSIWDPRRPLNTTEGQATLEVASDSYHKVASDVALLCGLRAQVYKFSISWSRIFPMGHGSSPSLPGVAYYNKLIDRLQDAGIEPMATLFHWDLPQALQDHGGWQNESVVDAFLDYAAFCFSTFGDRVKLWVTFHEPWVMSYAGYGTGQHPPGISDPGVASFKVAHLVLKAHARTWHHYNSHHRPQQQGHVGIVLNSDWAEPLSPERPEDLRASERFLHFMLGWFAHPVFVDGDYPATLRTQIQQMNRQCSHPVAQLPEFTEAEKQLLKGSADFLGLSHYTSRLISNAPQNTCIPSYDTIGGFSQHVNHVWPQTSSSWIRVVPWGIRRLLQFVSLEYTRGKVPIYLAGNGMPIGESENLFDDSLRVDYFNQYINEVLKAIKEDSVDVRSYIARSLIDGFEGPSGYSQRFGLHHVNFSDSSKSRTPRKSAYFFTSIIEKNGFLTKGAKRLLPPNTVNLPSKVRAFTFPSEVPSKAKVVWEKFSSQPKFERDLFYHGTFRDDFLWGVSSSAYQIEGAWDADGKGPSIWDNFTHTPGSNVKDNATGDIACDSYHQLDADLNMLRALKVKAYRFSISWSRIFPTGRNSSINSHGVDYYNRLINGLVASNIFPMVTLFHWDLPQALQDIGGWENPALIDLFDSYADFCFQTFGDRVKFWMTFNEPMYLAWLGYGSGEFPPGVKDPGWAPYRIAHAVIKAHARVYHTYDEKYRQEQKGVISLSLSTHWAEPKSPGVPRDVEAADRMLQFSLGWFAHPIFRNGDYPDTMKWKVGNRSELQHLATSRLPSFTEEEKRFIRATADVFCLNTYYSRIVQHKTPRLNPPSYEDDQEMAEEEDPSWPSTAMNRAAPWGTRRLLNWIKEEYGDIPIYITENGVGLTNPNTEDTDRIFYHKTYINEALKAYRLDGIDLRGYVAWSLMDNFEWLNGYTVKFGLYHVDFNNTNRPRTARASARYYTEVITNNGMPLAREDEFLYGRFPEGFIWSAASAAYQIEGAWRADGKGLSIWDTFSHTPLRVENDAIGDVACDSYHKIAEDLVTLQNLGVSHYRFSISWSRILPDGTTRYINEAGLNYYVRLIDTLLAASIQPQVTIYHWDLPQTLQDVGGWENETIVQRFKEYADVLFQRLGDKVKFWITLNEPFVIAYQGYGYGTAAPGVSNRPGTAPYIVGHNLIKAHAEAWHLYNDVYRASQGGVISITISSDWAEPRDPSNQEDVEAARRYVQFMGGWFAHPIFKNGDYNEVMKTRIRDRSLAAGLNKSRLPEFTESEKRRINGTYDFFGFNHYTTVLAYNLNYATAISSFDADRAS

Target ORF information:

RefSeq Version	XM_017004088.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lactase (LCT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004088.2

ORF Insert Sequence:

ATGGAGCTGT CTTGGCATGT AGTCTTTATT GCCCTGCTAA GTTTTTCATG CTGGGGGTCA 
GACTGGGAGT CTGATAGAAA TTTCATTTCC ACCGCTGGTC CTCTAACCAA TGACTTGCTG 
CACAACCTGA GTGGTCTCCT GGGAGACCAG AGTTCTAACT TTGTAGCAGG GGACAAAGAC 
ATGTATGTTT GTCACCAGCC ACTGCCCACT TTCCTGCCAG AATACTTCAG CAGTCTCCAT 
GCCAGTCAGA TCACCCATTA TAAGGTATTT CTGTCATGGG CACAGCTCCT CCCAGCAGGA 
AGCACCCAGA ATCCAGACGA GAAAACAGTG CAGTGCTACC GGCGACTCCT CAAGGCCCTC 
AAGACTGCAC GGCTTCAGCC CATGGTCATC CTGCACCACC AGACCCTCCC TGCCAGCACC 
CTCCGGAGAA CCGAAGCCTT TGCTGACCTC TTCGCCGACT ATGCCACATT CGCCTTCCAC 
TCCTTCGGGG ACCTAGTTGG GATCTGGTTC ACCTTCAGTG ACTTGGAGGA AGTGATCAAG 
GAGCTTCCCC ACCAGGAATC AAGAGCGTCA CAACTCCAGA CCCTCAGTGA TGCCCACAGA 
AAAGCCTATG AGATTTACCA CGAAAGCTAT GCTTTTCAGG GCGGAAAACT CTCTGTTGTC 
CTGCGAGCTG AAGATATCCC GGAGCTCCTG CTAGAACCAC CCATATCTGC GCTTGCCCAG 
GACACGGTCG ATTTCCTCTC TCTTGATTTG TCTTATGAAT GCCAAAATGA GGCAAGTCTG 
CGGCAGAAGC TGAGTAAATT GCAGACCATT GAGCCAAAAG TGAAAGTTTT CATCTTCAAC 
CTAAAACTCC CAGACTGCCC CTCCACCATG AAGAACCCAG CCAGTCTGCT CTTCAGCCTT 
TTTGAAGCCA TAAATAAAGA CCAAGTGCTC ACCATTGGGT TTGATATTAA TGAGTTTCTG 
AGTTGTTCAT CAAGTTCCAA GAAAAGCATG TCTTGTTCTC TGACTGGCAG CCTGGCCCTT 
CAGCCTGACC AGCAGCAGGA CCACGAGACC ACGGACTCCT CTCCTGCCTC TGCCTATCAG 
AGAATCTGGG AAGCATTTGC CAATCAGTCC AGGGCGGAAA GGGATGCCTT CCTGCAGGAT 
ACTTTCCCTG AAGGCTTCCT CTGGGGTGCC TCCACAGGAG CCTTTAACGT GGAAGGAGGC 
TGGGCCGAGG GTGGGAGAGG GGTGAGCATC TGGGATCCAC GCAGGCCCCT GAACACCACT 
GAGGGCCAAG CGACGCTGGA GGTGGCCAGC GACAGTTACC ACAAGGTAGC CTCTGACGTC 
GCCCTGCTTT GCGGCCTCCG GGCTCAGGTG TACAAGTTCT CCATCTCCTG GTCCCGGATC 
TTCCCCATGG GGCACGGGAG CAGCCCCAGC CTCCCAGGCG TTGCCTACTA CAACAAGCTG 
ATTGACAGGC TACAGGATGC GGGCATCGAG CCCATGGCCA CGCTGTTCCA CTGGGACCTG 
CCTCAGGCCC TGCAGGATCA TGGTGGATGG CAGAATGAGA GCGTGGTGGA TGCCTTCCTG 
GACTATGCGG CCTTCTGCTT CTCCACATTT GGGGACCGTG TGAAGCTGTG GGTGACCTTC 
CATGAGCCGT GGGTGATGAG CTACGCAGGC TATGGCACCG GCCAGCACCC TCCCGGCATC 
TCTGACCCAG GAGTGGCCTC TTTTAAGGTG GCTCACTTGG TCCTCAAGGC TCATGCCAGA 
ACTTGGCACC ACTACAACAG CCATCATCGC CCACAGCAGC AGGGGCACGT GGGCATTGTG 
CTGAACTCAG ACTGGGCAGA ACCCCTGTCT CCAGAGAGGC CTGAGGACCT GAGAGCCTCT 
GAGCGCTTCT TGCACTTCAT GCTGGGCTGG TTTGCACACC CCGTCTTTGT GGATGGAGAC 
TACCCAGCCA CCCTGAGGAC CCAGATCCAA CAGATGAACA GACAGTGCTC CCATCCTGTG 
GCTCAACTCC CCGAGTTCAC AGAGGCAGAG AAGCAGCTCC TGAAAGGCTC TGCTGATTTT 
CTGGGTCTGT CGCATTACAC CTCCCGCCTC ATCAGCAACG CCCCACAAAA CACCTGCATC 
CCTAGCTATG ATACCATTGG AGGCTTCTCC CAACACGTGA ACCATGTGTG GCCCCAGACC 
TCATCCTCTT GGATTCGTGT GGTGCCCTGG GGGATAAGGA GGCTGTTGCA GTTTGTATCC 
CTGGAATACA CAAGAGGAAA AGTTCCAATA TACCTTGCCG GGAATGGCAT GCCCATAGGG 
GAAAGTGAAA ATCTCTTTGA TGATTCCTTA AGAGTAGACT ACTTCAATCA ATATATCAAT 
GAGGTGCTCA AGGCTATCAA GGAAGACTCT GTGGATGTTC GTTCCTACAT TGCTCGTTCC 
CTCATTGATG GCTTCGAAGG CCCTTCTGGT TACAGCCAGC GGTTTGGCCT GCACCACGTC 
AACTTCAGCG ACAGCAGCAA GTCAAGGACT CCCAGGAAAT CTGCCTACTT TTTCACTAGC 
ATCATAGAAA AGAACGGTTT CCTCACCAAG GGGGCAAAAA GACTGCTACC ACCTAATACA 
GTAAACCTCC CCTCCAAAGT CAGAGCCTTC ACTTTTCCAT CTGAGGTGCC CTCCAAGGCT 
AAAGTCGTTT GGGAAAAGTT CTCCAGCCAA CCCAAGTTCG AAAGAGATTT GTTCTACCAC 
GGGACGTTTC GGGATGACTT TCTGTGGGGC GTGTCCTCTT CCGCTTATCA GATTGAAGGC 
GCGTGGGATG CCGATGGCAA AGGCCCCAGC ATCTGGGATA ACTTTACCCA CACACCAGGG 
AGCAATGTGA AAGACAATGC CACTGGAGAC ATCGCCTGTG ACAGCTATCA CCAGCTGGAT 
GCCGATCTGA ATATGCTCCG AGCTTTGAAG GTGAAGGCCT ACCGCTTCTC TATCTCCTGG 
TCTCGGATTT TCCCAACTGG GAGAAACAGC TCTATCAACA GTCATGGGGT TGATTATTAC 
AACAGGCTGA TCAATGGCTT GGTGGCAAGC AACATCTTTC CCATGGTGAC ATTGTTCCAT 
TGGGACCTGC CCCAGGCCCT CCAGGATATC GGAGGCTGGG AGAATCCTGC CTTGATTGAC 
TTGTTTGACA GCTACGCAGA CTTTTGTTTC CAGACCTTTG GTGATAGAGT CAAGTTTTGG 
ATGACTTTTA ATGAGCCCAT GTACCTGGCA TGGCTAGGTT ATGGCTCAGG GGAATTTCCC 
CCAGGGGTGA AGGACCCAGG CTGGGCACCA TATAGGATAG CCCACGCCGT CATCAAAGCC 
CATGCCAGAG TCTATCACAC GTACGATGAG AAATACAGGC AGGAGCAGAA GGGGGTCATC 
TCGCTGAGCC TCAGTACACA CTGGGCAGAG CCCAAGTCAC CAGGGGTCCC CAGAGATGTG 
GAAGCCGCTG ACCGAATGCT GCAGTTCTCC CTGGGCTGGT TTGCTCACCC CATTTTTAGA 
AACGGAGACT ATCCTGACAC CATGAAGTGG AAAGTGGGGA ACAGGAGTGA ACTGCAGCAC 
TTAGCCACCT CCCGCCTGCC AAGCTTCACT GAGGAAGAGA AGAGGTTCAT CAGGGCGACG 
GCCGACGTCT TCTGCCTCAA CACGTACTAC TCCAGAATCG TGCAGCACAA AACACCCAGG 
CTAAACCCAC CCTCCTACGA AGACGACCAG GAGATGGCTG AGGAGGAGGA CCCTTCGTGG 
CCTTCCACGG CAATGAACAG AGCTGCGCCC TGGGGGACGC GAAGGCTGCT GAACTGGATC 
AAGGAAGAGT ATGGTGACAT CCCCATTTAC ATCACCGAAA ACGGAGTGGG GCTGACCAAT 
CCGAACACGG AGGATACTGA TAGGATATTT TACCACAAAA CCTACATCAA TGAGGCTTTG 
AAAGCCTACA GGCTCGATGG TATAGACCTT CGAGGGTATG TCGCCTGGTC TCTGATGGAC 
AACTTTGAGT GGCTAAATGG CTACACGGTC AAGTTTGGAC TGTACCATGT TGATTTCAAC 
AACACGAACA GGCCTCGCAC AGCAAGAGCC TCCGCCAGGT ACTACACAGA GGTCATTACC 
AACAACGGCA TGCCACTGGC CAGGGAGGAT GAGTTTCTGT ACGGACGGTT TCCTGAGGGC 
TTCATCTGGA GTGCAGCTTC TGCTGCATAT CAGATTGAAG GTGCGTGGAG AGCAGATGGC 
AAAGGACTCA GCATTTGGGA CACGTTTTCT CACACACCAC TGAGGGTTGA GAACGATGCC 
ATTGGAGACG TGGCCTGTGA CAGTTATCAC AAGATTGCTG AGGATCTGGT CACCCTGCAG 
AACCTGGGCG TGTCCCACTA CCGTTTTTCC ATCTCCTGGT CTCGCATCCT CCCTGATGGA 
ACCACCAGGT ACATCAATGA AGCGGGCCTG AACTACTACG TGAGGCTCAT CGATACACTG 
CTGGCCGCCA GCATCCAGCC CCAGGTGACC ATTTACCACT GGGACCTACC ACAGACGCTC 
CAAGATGTAG GAGGCTGGGA GAATGAGACC ATCGTGCAGC GGTTTAAGGA GTATGCAGAT 
GTGCTCTTCC AGAGGCTGGG AGACAAGGTG AAGTTTTGGA TCACGCTGAA TGAGCCCTTT 
GTCATTGCTT ACCAGGGCTA TGGCTACGGA ACAGCAGCTC CAGGAGTCTC CAATAGGCCT 
GGCACTGCCC CCTACATTGT TGGCCACAAT CTAATAAAGG CTCATGCTGA GGCCTGGCAT 
CTGTACAACG ATGTGTACCG CGCCAGTCAA GGTGGCGTGA TTTCCATCAC CATCAGCAGT 
GACTGGGCTG AACCCAGAGA TCCCTCTAAC CAGGAGGATG TGGAGGCAGC CAGGAGATAT 
GTTCAGTTCA TGGGAGGCTG GTTTGCACAT CCTATTTTCA AGAATGGAGA TTACAATGAG 
GTGATGAAGA CGCGGATCCG TGACAGGAGC TTGGCTGCAG GCCTCAACAA GTCTCGGCTG 
CCAGAATTTA CAGAGAGTGA GAAGAGGAGG ATCAACGGCA CCTATGACTT TTTTGGGTTC 
AATCACTACA CCACTGTCCT CGCCTACAAC CTCAACTATG CCACTGCCAT CTCTTCTTTT 
GATGCAGACA GGGCCTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LCT ( XM_017004088.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859577.1 Homo sapiens lactase (LCT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu89445
Clone ID Related Accession (Same CDS sequence)	XM_017004088.2 , XM_017004088.1
Accession Version	XM_017004088.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lactase-phlorizin hydrolase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGT CTTGGCATGT AGTCTTTATT GCCCTGCTAA GTTTTTCATG CTGGGGGTCA 
GACTGGGAGT CTGATAGAAA TTTCATTTCC ACCGCTGGTC CTCTAACCAA TGACTTGCTG 
CACAACCTGA GTGGTCTCCT GGGAGACCAG AGTTCTAACT TTGTAGCAGG GGACAAAGAC 
ATGTATGTTT GTCACCAGCC ACTGCCCACT TTCCTGCCAG AATACTTCAG CAGTCTCCAT 
GCCAGTCAGA TCACCCATTA TAAGGTATTT CTGTCATGGG CACAGCTCCT CCCAGCAGGA 
AGCACCCAGA ATCCAGACGA GAAAACAGTG CAGTGCTACC GGCGACTCCT CAAGGCCCTC 
AAGACTGCAC GGCTTCAGCC CATGGTCATC CTGCACCACC AGACCCTCCC TGCCAGCACC 
CTCCGGAGAA CCGAAGCCTT TGCTGACCTC TTCGCCGACT ATGCCACATT CGCCTTCCAC 
TCCTTCGGGG ACCTAGTTGG GATCTGGTTC ACCTTCAGTG ACTTGGAGGA AGTGATCAAG 
GAGCTTCCCC ACCAGGAATC AAGAGCGTCA CAACTCCAGA CCCTCAGTGA TGCCCACAGA 
AAAGCCTATG AGATTTACCA CGAAAGCTAT GCTTTTCAGG GCGGAAAACT CTCTGTTGTC 
CTGCGAGCTG AAGATATCCC GGAGCTCCTG CTAGAACCAC CCATATCTGC GCTTGCCCAG 
GACACGGTCG ATTTCCTCTC TCTTGATTTG TCTTATGAAT GCCAAAATGA GGCAAGTCTG 
CGGCAGAAGC TGAGTAAATT GCAGACCATT GAGCCAAAAG TGAAAGTTTT CATCTTCAAC 
CTAAAACTCC CAGACTGCCC CTCCACCATG AAGAACCCAG CCAGTCTGCT CTTCAGCCTT 
TTTGAAGCCA TAAATAAAGA CCAAGTGCTC ACCATTGGGT TTGATATTAA TGAGTTTCTG 
AGTTGTTCAT CAAGTTCCAA GAAAAGCATG TCTTGTTCTC TGACTGGCAG CCTGGCCCTT 
CAGCCTGACC AGCAGCAGGA CCACGAGACC ACGGACTCCT CTCCTGCCTC TGCCTATCAG 
AGAATCTGGG AAGCATTTGC CAATCAGTCC AGGGCGGAAA GGGATGCCTT CCTGCAGGAT 
ACTTTCCCTG AAGGCTTCCT CTGGGGTGCC TCCACAGGAG CCTTTAACGT GGAAGGAGGC 
TGGGCCGAGG GTGGGAGAGG GGTGAGCATC TGGGATCCAC GCAGGCCCCT GAACACCACT 
GAGGGCCAAG CGACGCTGGA GGTGGCCAGC GACAGTTACC ACAAGGTAGC CTCTGACGTC 
GCCCTGCTTT GCGGCCTCCG GGCTCAGGTG TACAAGTTCT CCATCTCCTG GTCCCGGATC 
TTCCCCATGG GGCACGGGAG CAGCCCCAGC CTCCCAGGCG TTGCCTACTA CAACAAGCTG 
ATTGACAGGC TACAGGATGC GGGCATCGAG CCCATGGCCA CGCTGTTCCA CTGGGACCTG 
CCTCAGGCCC TGCAGGATCA TGGTGGATGG CAGAATGAGA GCGTGGTGGA TGCCTTCCTG 
GACTATGCGG CCTTCTGCTT CTCCACATTT GGGGACCGTG TGAAGCTGTG GGTGACCTTC 
CATGAGCCGT GGGTGATGAG CTACGCAGGC TATGGCACCG GCCAGCACCC TCCCGGCATC 
TCTGACCCAG GAGTGGCCTC TTTTAAGGTG GCTCACTTGG TCCTCAAGGC TCATGCCAGA 
ACTTGGCACC ACTACAACAG CCATCATCGC CCACAGCAGC AGGGGCACGT GGGCATTGTG 
CTGAACTCAG ACTGGGCAGA ACCCCTGTCT CCAGAGAGGC CTGAGGACCT GAGAGCCTCT 
GAGCGCTTCT TGCACTTCAT GCTGGGCTGG TTTGCACACC CCGTCTTTGT GGATGGAGAC 
TACCCAGCCA CCCTGAGGAC CCAGATCCAA CAGATGAACA GACAGTGCTC CCATCCTGTG 
GCTCAACTCC CCGAGTTCAC AGAGGCAGAG AAGCAGCTCC TGAAAGGCTC TGCTGATTTT 
CTGGGTCTGT CGCATTACAC CTCCCGCCTC ATCAGCAACG CCCCACAAAA CACCTGCATC 
CCTAGCTATG ATACCATTGG AGGCTTCTCC CAACACGTGA ACCATGTGTG GCCCCAGACC 
TCATCCTCTT GGATTCGTGT GGTGCCCTGG GGGATAAGGA GGCTGTTGCA GTTTGTATCC 
CTGGAATACA CAAGAGGAAA AGTTCCAATA TACCTTGCCG GGAATGGCAT GCCCATAGGG 
GAAAGTGAAA ATCTCTTTGA TGATTCCTTA AGAGTAGACT ACTTCAATCA ATATATCAAT 
GAGGTGCTCA AGGCTATCAA GGAAGACTCT GTGGATGTTC GTTCCTACAT TGCTCGTTCC 
CTCATTGATG GCTTCGAAGG CCCTTCTGGT TACAGCCAGC GGTTTGGCCT GCACCACGTC 
AACTTCAGCG ACAGCAGCAA GTCAAGGACT CCCAGGAAAT CTGCCTACTT TTTCACTAGC 
ATCATAGAAA AGAACGGTTT CCTCACCAAG GGGGCAAAAA GACTGCTACC ACCTAATACA 
GTAAACCTCC CCTCCAAAGT CAGAGCCTTC ACTTTTCCAT CTGAGGTGCC CTCCAAGGCT 
AAAGTCGTTT GGGAAAAGTT CTCCAGCCAA CCCAAGTTCG AAAGAGATTT GTTCTACCAC 
GGGACGTTTC GGGATGACTT TCTGTGGGGC GTGTCCTCTT CCGCTTATCA GATTGAAGGC 
GCGTGGGATG CCGATGGCAA AGGCCCCAGC ATCTGGGATA ACTTTACCCA CACACCAGGG 
AGCAATGTGA AAGACAATGC CACTGGAGAC ATCGCCTGTG ACAGCTATCA CCAGCTGGAT 
GCCGATCTGA ATATGCTCCG AGCTTTGAAG GTGAAGGCCT ACCGCTTCTC TATCTCCTGG 
TCTCGGATTT TCCCAACTGG GAGAAACAGC TCTATCAACA GTCATGGGGT TGATTATTAC 
AACAGGCTGA TCAATGGCTT GGTGGCAAGC AACATCTTTC CCATGGTGAC ATTGTTCCAT 
TGGGACCTGC CCCAGGCCCT CCAGGATATC GGAGGCTGGG AGAATCCTGC CTTGATTGAC 
TTGTTTGACA GCTACGCAGA CTTTTGTTTC CAGACCTTTG GTGATAGAGT CAAGTTTTGG 
ATGACTTTTA ATGAGCCCAT GTACCTGGCA TGGCTAGGTT ATGGCTCAGG GGAATTTCCC 
CCAGGGGTGA AGGACCCAGG CTGGGCACCA TATAGGATAG CCCACGCCGT CATCAAAGCC 
CATGCCAGAG TCTATCACAC GTACGATGAG AAATACAGGC AGGAGCAGAA GGGGGTCATC 
TCGCTGAGCC TCAGTACACA CTGGGCAGAG CCCAAGTCAC CAGGGGTCCC CAGAGATGTG 
GAAGCCGCTG ACCGAATGCT GCAGTTCTCC CTGGGCTGGT TTGCTCACCC CATTTTTAGA 
AACGGAGACT ATCCTGACAC CATGAAGTGG AAAGTGGGGA ACAGGAGTGA ACTGCAGCAC 
TTAGCCACCT CCCGCCTGCC AAGCTTCACT GAGGAAGAGA AGAGGTTCAT CAGGGCGACG 
GCCGACGTCT TCTGCCTCAA CACGTACTAC TCCAGAATCG TGCAGCACAA AACACCCAGG 
CTAAACCCAC CCTCCTACGA AGACGACCAG GAGATGGCTG AGGAGGAGGA CCCTTCGTGG 
CCTTCCACGG CAATGAACAG AGCTGCGCCC TGGGGGACGC GAAGGCTGCT GAACTGGATC 
AAGGAAGAGT ATGGTGACAT CCCCATTTAC ATCACCGAAA ACGGAGTGGG GCTGACCAAT 
CCGAACACGG AGGATACTGA TAGGATATTT TACCACAAAA CCTACATCAA TGAGGCTTTG 
AAAGCCTACA GGCTCGATGG TATAGACCTT CGAGGGTATG TCGCCTGGTC TCTGATGGAC 
AACTTTGAGT GGCTAAATGG CTACACGGTC AAGTTTGGAC TGTACCATGT TGATTTCAAC 
AACACGAACA GGCCTCGCAC AGCAAGAGCC TCCGCCAGGT ACTACACAGA GGTCATTACC 
AACAACGGCA TGCCACTGGC CAGGGAGGAT GAGTTTCTGT ACGGACGGTT TCCTGAGGGC 
TTCATCTGGA GTGCAGCTTC TGCTGCATAT CAGATTGAAG GTGCGTGGAG AGCAGATGGC 
AAAGGACTCA GCATTTGGGA CACGTTTTCT CACACACCAC TGAGGGTTGA GAACGATGCC 
ATTGGAGACG TGGCCTGTGA CAGTTATCAC AAGATTGCTG AGGATCTGGT CACCCTGCAG 
AACCTGGGCG TGTCCCACTA CCGTTTTTCC ATCTCCTGGT CTCGCATCCT CCCTGATGGA 
ACCACCAGGT ACATCAATGA AGCGGGCCTG AACTACTACG TGAGGCTCAT CGATACACTG 
CTGGCCGCCA GCATCCAGCC CCAGGTGACC ATTTACCACT GGGACCTACC ACAGACGCTC 
CAAGATGTAG GAGGCTGGGA GAATGAGACC ATCGTGCAGC GGTTTAAGGA GTATGCAGAT 
GTGCTCTTCC AGAGGCTGGG AGACAAGGTG AAGTTTTGGA TCACGCTGAA TGAGCCCTTT 
GTCATTGCTT ACCAGGGCTA TGGCTACGGA ACAGCAGCTC CAGGAGTCTC CAATAGGCCT 
GGCACTGCCC CCTACATTGT TGGCCACAAT CTAATAAAGG CTCATGCTGA GGCCTGGCAT 
CTGTACAACG ATGTGTACCG CGCCAGTCAA GGTGGCGTGA TTTCCATCAC CATCAGCAGT 
GACTGGGCTG AACCCAGAGA TCCCTCTAAC CAGGAGGATG TGGAGGCAGC CAGGAGATAT 
GTTCAGTTCA TGGGAGGCTG GTTTGCACAT CCTATTTTCA AGAATGGAGA TTACAATGAG 
GTGATGAAGA CGCGGATCCG TGACAGGAGC TTGGCTGCAG GCCTCAACAA GTCTCGGCTG 
CCAGAATTTA CAGAGAGTGA GAAGAGGAGG ATCAACGGCA CCTATGACTT TTTTGGGTTC 
AATCACTACA CCACTGTCCT CGCCTACAAC CTCAACTATG CCACTGCCAT CTCTTCTTTT 
GATGCAGACA GGGCCTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859577.1
CDS	32..5152
Translation	MELSWHVVFIALLSFSCWGSDWESDRNFISTAGPLTNDLLHNLSGLLGDQSSNFVAGDKDMYVCHQPLPTFLPEYFSSLHASQITHYKVFLSWAQLLPAGSTQNPDEKTVQCYRRLLKALKTARLQPMVILHHQTLPASTLRRTEAFADLFADYATFAFHSFGDLVGIWFTFSDLEEVIKELPHQESRASQLQTLSDAHRKAYEIYHESYAFQGGKLSVVLRAEDIPELLLEPPISALAQDTVDFLSLDLSYECQNEASLRQKLSKLQTIEPKVKVFIFNLKLPDCPSTMKNPASLLFSLFEAINKDQVLTIGFDINEFLSCSSSSKKSMSCSLTGSLALQPDQQQDHETTDSSPASAYQRIWEAFANQSRAERDAFLQDTFPEGFLWGASTGAFNVEGGWAEGGRGVSIWDPRRPLNTTEGQATLEVASDSYHKVASDVALLCGLRAQVYKFSISWSRIFPMGHGSSPSLPGVAYYNKLIDRLQDAGIEPMATLFHWDLPQALQDHGGWQNESVVDAFLDYAAFCFSTFGDRVKLWVTFHEPWVMSYAGYGTGQHPPGISDPGVASFKVAHLVLKAHARTWHHYNSHHRPQQQGHVGIVLNSDWAEPLSPERPEDLRASERFLHFMLGWFAHPVFVDGDYPATLRTQIQQMNRQCSHPVAQLPEFTEAEKQLLKGSADFLGLSHYTSRLISNAPQNTCIPSYDTIGGFSQHVNHVWPQTSSSWIRVVPWGIRRLLQFVSLEYTRGKVPIYLAGNGMPIGESENLFDDSLRVDYFNQYINEVLKAIKEDSVDVRSYIARSLIDGFEGPSGYSQRFGLHHVNFSDSSKSRTPRKSAYFFTSIIEKNGFLTKGAKRLLPPNTVNLPSKVRAFTFPSEVPSKAKVVWEKFSSQPKFERDLFYHGTFRDDFLWGVSSSAYQIEGAWDADGKGPSIWDNFTHTPGSNVKDNATGDIACDSYHQLDADLNMLRALKVKAYRFSISWSRIFPTGRNSSINSHGVDYYNRLINGLVASNIFPMVTLFHWDLPQALQDIGGWENPALIDLFDSYADFCFQTFGDRVKFWMTFNEPMYLAWLGYGSGEFPPGVKDPGWAPYRIAHAVIKAHARVYHTYDEKYRQEQKGVISLSLSTHWAEPKSPGVPRDVEAADRMLQFSLGWFAHPIFRNGDYPDTMKWKVGNRSELQHLATSRLPSFTEEEKRFIRATADVFCLNTYYSRIVQHKTPRLNPPSYEDDQEMAEEEDPSWPSTAMNRAAPWGTRRLLNWIKEEYGDIPIYITENGVGLTNPNTEDTDRIFYHKTYINEALKAYRLDGIDLRGYVAWSLMDNFEWLNGYTVKFGLYHVDFNNTNRPRTARASARYYTEVITNNGMPLAREDEFLYGRFPEGFIWSAASAAYQIEGAWRADGKGLSIWDTFSHTPLRVENDAIGDVACDSYHKIAEDLVTLQNLGVSHYRFSISWSRILPDGTTRYINEAGLNYYVRLIDTLLAASIQPQVTIYHWDLPQTLQDVGGWENETIVQRFKEYADVLFQRLGDKVKFWITLNEPFVIAYQGYGYGTAAPGVSNRPGTAPYIVGHNLIKAHAEAWHLYNDVYRASQGGVISITISSDWAEPRDPSNQEDVEAARRYVQFMGGWFAHPIFKNGDYNEVMKTRIRDRSLAAGLNKSRLPEFTESEKRRINGTYDFFGFNHYTTVLAYNLNYATAISSFDADRAS

Target ORF information:

RefSeq Version	XM_017004088.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lactase (LCT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004088.1

ORF Insert Sequence:

ATGGAGCTGT CTTGGCATGT AGTCTTTATT GCCCTGCTAA GTTTTTCATG CTGGGGGTCA 
GACTGGGAGT CTGATAGAAA TTTCATTTCC ACCGCTGGTC CTCTAACCAA TGACTTGCTG 
CACAACCTGA GTGGTCTCCT GGGAGACCAG AGTTCTAACT TTGTAGCAGG GGACAAAGAC 
ATGTATGTTT GTCACCAGCC ACTGCCCACT TTCCTGCCAG AATACTTCAG CAGTCTCCAT 
GCCAGTCAGA TCACCCATTA TAAGGTATTT CTGTCATGGG CACAGCTCCT CCCAGCAGGA 
AGCACCCAGA ATCCAGACGA GAAAACAGTG CAGTGCTACC GGCGACTCCT CAAGGCCCTC 
AAGACTGCAC GGCTTCAGCC CATGGTCATC CTGCACCACC AGACCCTCCC TGCCAGCACC 
CTCCGGAGAA CCGAAGCCTT TGCTGACCTC TTCGCCGACT ATGCCACATT CGCCTTCCAC 
TCCTTCGGGG ACCTAGTTGG GATCTGGTTC ACCTTCAGTG ACTTGGAGGA AGTGATCAAG 
GAGCTTCCCC ACCAGGAATC AAGAGCGTCA CAACTCCAGA CCCTCAGTGA TGCCCACAGA 
AAAGCCTATG AGATTTACCA CGAAAGCTAT GCTTTTCAGG GCGGAAAACT CTCTGTTGTC 
CTGCGAGCTG AAGATATCCC GGAGCTCCTG CTAGAACCAC CCATATCTGC GCTTGCCCAG 
GACACGGTCG ATTTCCTCTC TCTTGATTTG TCTTATGAAT GCCAAAATGA GGCAAGTCTG 
CGGCAGAAGC TGAGTAAATT GCAGACCATT GAGCCAAAAG TGAAAGTTTT CATCTTCAAC 
CTAAAACTCC CAGACTGCCC CTCCACCATG AAGAACCCAG CCAGTCTGCT CTTCAGCCTT 
TTTGAAGCCA TAAATAAAGA CCAAGTGCTC ACCATTGGGT TTGATATTAA TGAGTTTCTG 
AGTTGTTCAT CAAGTTCCAA GAAAAGCATG TCTTGTTCTC TGACTGGCAG CCTGGCCCTT 
CAGCCTGACC AGCAGCAGGA CCACGAGACC ACGGACTCCT CTCCTGCCTC TGCCTATCAG 
AGAATCTGGG AAGCATTTGC CAATCAGTCC AGGGCGGAAA GGGATGCCTT CCTGCAGGAT 
ACTTTCCCTG AAGGCTTCCT CTGGGGTGCC TCCACAGGAG CCTTTAACGT GGAAGGAGGC 
TGGGCCGAGG GTGGGAGAGG GGTGAGCATC TGGGATCCAC GCAGGCCCCT GAACACCACT 
GAGGGCCAAG CGACGCTGGA GGTGGCCAGC GACAGTTACC ACAAGGTAGC CTCTGACGTC 
GCCCTGCTTT GCGGCCTCCG GGCTCAGGTG TACAAGTTCT CCATCTCCTG GTCCCGGATC 
TTCCCCATGG GGCACGGGAG CAGCCCCAGC CTCCCAGGCG TTGCCTACTA CAACAAGCTG 
ATTGACAGGC TACAGGATGC GGGCATCGAG CCCATGGCCA CGCTGTTCCA CTGGGACCTG 
CCTCAGGCCC TGCAGGATCA TGGTGGATGG CAGAATGAGA GCGTGGTGGA TGCCTTCCTG 
GACTATGCGG CCTTCTGCTT CTCCACATTT GGGGACCGTG TGAAGCTGTG GGTGACCTTC 
CATGAGCCGT GGGTGATGAG CTACGCAGGC TATGGCACCG GCCAGCACCC TCCCGGCATC 
TCTGACCCAG GAGTGGCCTC TTTTAAGGTG GCTCACTTGG TCCTCAAGGC TCATGCCAGA 
ACTTGGCACC ACTACAACAG CCATCATCGC CCACAGCAGC AGGGGCACGT GGGCATTGTG 
CTGAACTCAG ACTGGGCAGA ACCCCTGTCT CCAGAGAGGC CTGAGGACCT GAGAGCCTCT 
GAGCGCTTCT TGCACTTCAT GCTGGGCTGG TTTGCACACC CCGTCTTTGT GGATGGAGAC 
TACCCAGCCA CCCTGAGGAC CCAGATCCAA CAGATGAACA GACAGTGCTC CCATCCTGTG 
GCTCAACTCC CCGAGTTCAC AGAGGCAGAG AAGCAGCTCC TGAAAGGCTC TGCTGATTTT 
CTGGGTCTGT CGCATTACAC CTCCCGCCTC ATCAGCAACG CCCCACAAAA CACCTGCATC 
CCTAGCTATG ATACCATTGG AGGCTTCTCC CAACACGTGA ACCATGTGTG GCCCCAGACC 
TCATCCTCTT GGATTCGTGT GGTGCCCTGG GGGATAAGGA GGCTGTTGCA GTTTGTATCC 
CTGGAATACA CAAGAGGAAA AGTTCCAATA TACCTTGCCG GGAATGGCAT GCCCATAGGG 
GAAAGTGAAA ATCTCTTTGA TGATTCCTTA AGAGTAGACT ACTTCAATCA ATATATCAAT 
GAGGTGCTCA AGGCTATCAA GGAAGACTCT GTGGATGTTC GTTCCTACAT TGCTCGTTCC 
CTCATTGATG GCTTCGAAGG CCCTTCTGGT TACAGCCAGC GGTTTGGCCT GCACCACGTC 
AACTTCAGCG ACAGCAGCAA GTCAAGGACT CCCAGGAAAT CTGCCTACTT TTTCACTAGC 
ATCATAGAAA AGAACGGTTT CCTCACCAAG GGGGCAAAAA GACTGCTACC ACCTAATACA 
GTAAACCTCC CCTCCAAAGT CAGAGCCTTC ACTTTTCCAT CTGAGGTGCC CTCCAAGGCT 
AAAGTCGTTT GGGAAAAGTT CTCCAGCCAA CCCAAGTTCG AAAGAGATTT GTTCTACCAC 
GGGACGTTTC GGGATGACTT TCTGTGGGGC GTGTCCTCTT CCGCTTATCA GATTGAAGGC 
GCGTGGGATG CCGATGGCAA AGGCCCCAGC ATCTGGGATA ACTTTACCCA CACACCAGGG 
AGCAATGTGA AAGACAATGC CACTGGAGAC ATCGCCTGTG ACAGCTATCA CCAGCTGGAT 
GCCGATCTGA ATATGCTCCG AGCTTTGAAG GTGAAGGCCT ACCGCTTCTC TATCTCCTGG 
TCTCGGATTT TCCCAACTGG GAGAAACAGC TCTATCAACA GTCATGGGGT TGATTATTAC 
AACAGGCTGA TCAATGGCTT GGTGGCAAGC AACATCTTTC CCATGGTGAC ATTGTTCCAT 
TGGGACCTGC CCCAGGCCCT CCAGGATATC GGAGGCTGGG AGAATCCTGC CTTGATTGAC 
TTGTTTGACA GCTACGCAGA CTTTTGTTTC CAGACCTTTG GTGATAGAGT CAAGTTTTGG 
ATGACTTTTA ATGAGCCCAT GTACCTGGCA TGGCTAGGTT ATGGCTCAGG GGAATTTCCC 
CCAGGGGTGA AGGACCCAGG CTGGGCACCA TATAGGATAG CCCACGCCGT CATCAAAGCC 
CATGCCAGAG TCTATCACAC GTACGATGAG AAATACAGGC AGGAGCAGAA GGGGGTCATC 
TCGCTGAGCC TCAGTACACA CTGGGCAGAG CCCAAGTCAC CAGGGGTCCC CAGAGATGTG 
GAAGCCGCTG ACCGAATGCT GCAGTTCTCC CTGGGCTGGT TTGCTCACCC CATTTTTAGA 
AACGGAGACT ATCCTGACAC CATGAAGTGG AAAGTGGGGA ACAGGAGTGA ACTGCAGCAC 
TTAGCCACCT CCCGCCTGCC AAGCTTCACT GAGGAAGAGA AGAGGTTCAT CAGGGCGACG 
GCCGACGTCT TCTGCCTCAA CACGTACTAC TCCAGAATCG TGCAGCACAA AACACCCAGG 
CTAAACCCAC CCTCCTACGA AGACGACCAG GAGATGGCTG AGGAGGAGGA CCCTTCGTGG 
CCTTCCACGG CAATGAACAG AGCTGCGCCC TGGGGGACGC GAAGGCTGCT GAACTGGATC 
AAGGAAGAGT ATGGTGACAT CCCCATTTAC ATCACCGAAA ACGGAGTGGG GCTGACCAAT 
CCGAACACGG AGGATACTGA TAGGATATTT TACCACAAAA CCTACATCAA TGAGGCTTTG 
AAAGCCTACA GGCTCGATGG TATAGACCTT CGAGGGTATG TCGCCTGGTC TCTGATGGAC 
AACTTTGAGT GGCTAAATGG CTACACGGTC AAGTTTGGAC TGTACCATGT TGATTTCAAC 
AACACGAACA GGCCTCGCAC AGCAAGAGCC TCCGCCAGGT ACTACACAGA GGTCATTACC 
AACAACGGCA TGCCACTGGC CAGGGAGGAT GAGTTTCTGT ACGGACGGTT TCCTGAGGGC 
TTCATCTGGA GTGCAGCTTC TGCTGCATAT CAGATTGAAG GTGCGTGGAG AGCAGATGGC 
AAAGGACTCA GCATTTGGGA CACGTTTTCT CACACACCAC TGAGGGTTGA GAACGATGCC 
ATTGGAGACG TGGCCTGTGA CAGTTATCAC AAGATTGCTG AGGATCTGGT CACCCTGCAG 
AACCTGGGCG TGTCCCACTA CCGTTTTTCC ATCTCCTGGT CTCGCATCCT CCCTGATGGA 
ACCACCAGGT ACATCAATGA AGCGGGCCTG AACTACTACG TGAGGCTCAT CGATACACTG 
CTGGCCGCCA GCATCCAGCC CCAGGTGACC ATTTACCACT GGGACCTACC ACAGACGCTC 
CAAGATGTAG GAGGCTGGGA GAATGAGACC ATCGTGCAGC GGTTTAAGGA GTATGCAGAT 
GTGCTCTTCC AGAGGCTGGG AGACAAGGTG AAGTTTTGGA TCACGCTGAA TGAGCCCTTT 
GTCATTGCTT ACCAGGGCTA TGGCTACGGA ACAGCAGCTC CAGGAGTCTC CAATAGGCCT 
GGCACTGCCC CCTACATTGT TGGCCACAAT CTAATAAAGG CTCATGCTGA GGCCTGGCAT 
CTGTACAACG ATGTGTACCG CGCCAGTCAA GGTGGCGTGA TTTCCATCAC CATCAGCAGT 
GACTGGGCTG AACCCAGAGA TCCCTCTAAC CAGGAGGATG TGGAGGCAGC CAGGAGATAT 
GTTCAGTTCA TGGGAGGCTG GTTTGCACAT CCTATTTTCA AGAATGGAGA TTACAATGAG 
GTGATGAAGA CGCGGATCCG TGACAGGAGC TTGGCTGCAG GCCTCAACAA GTCTCGGCTG 
CCAGAATTTA CAGAGAGTGA GAAGAGGAGG ATCAACGGCA CCTATGACTT TTTTGGGTTC 
AATCACTACA CCACTGTCCT CGCCTACAAC CTCAACTATG CCACTGCCAT CTCTTCTTTT 
GATGCAGACA GGGCCTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LCT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

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