HES5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HES5
Entrez Gene ID	388585
Full Name	hes family bHLH transcription factor 5
Synonyms	bHLHb38
General protein information	Preferred Names hes family bHLH transcription factor 5 Names transcription factor HES-5 class B basic helix-loop-helix protein 38 hairy and enhancer of split 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.32
Summary	This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer. [provided by RefSeq, Dec 2008].
Disorder MIM:	607348

mRNA and Protein(s)

mRNA	Protein	Name
NM_001010926.4	NP_001010926.1	transcription factor HES-5
NM_001010926.3	NP_001010926.1	transcription factor HES-5
XM_005244751.4	XP_005244808.1	transcription factor HES-5 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100149066	NP_001121862.1
Homo sapiens (human)	HES5	NP_001010926.1
Mus musculus (house mouse)	Hes5	NP_034549.1
Danio rerio (zebrafish)	her4.2	NP_571165.3
Danio rerio (zebrafish)	her4.3	NP_001154880.1
Xenopus tropicalis (tropical clawed frog)	hes5	XP_004916212.1
Pan troglodytes (chimpanzee)	HES5	XP_524847.2
Canis lupus familiaris (dog)	HES5	XP_546734.2
Bos taurus (cattle)	HES5	NP_001098937.1
Rattus norvegicus (Norway rat)	Hes5	NP_077359.1
Gallus gallus (chicken)	HES5	NP_001012713.1
Xenopus tropicalis (tropical clawed frog)	hes5.1	NP_001037880.1
Danio rerio (zebrafish)	LOC100149863	NP_001096598.1
Danio rerio (zebrafish)	LOC100148329	NP_001154881.1

Gene Ontology
Bibliography

Related articles in PubMed

HES5 promotes cellular proliferation of non-small cell lung cancer through STAT3 signaling.
Gu S, Zhang R, Gu J, Li X, Lv L, Jiang J, Xu Z, Wang S, Shi C, Wang DP, Wu C
Oncology reports37(1)474-482(2017 Jan)

HES5 promotes cell proliferation and invasion through activation of STAT3 and predicts poor survival in hepatocellular carcinoma.
Zhu G, Shi W, Fan H, Zhang X, Xu J, Chen Y, Xu Z, Tao T, Cheng C
Experimental and molecular pathology99(3)474-84(2015 Dec)

HES5 silencing is an early and recurrent change in prostate tumourigenesis.
Massie CE, Spiteri I, Ross-Adams H, Luxton H, Kay J, Whitaker HC, Dunning MJ, Lamb AD, Ramos-Montoya A, Brewer DS, Cooper CS, Eeles R, , Warren AY, Tavar? S, Neal DE, Lynch AG
Endocrine-related cancer22(2)131-44(2015 Apr)

HES5 is a key mediator of Wnt-3a-induced neuronal differentiation.
Mu?mann C, H?bner R, Trilck M, Rolfs A, Frech MJ
Stem cells and development23(12)1328-39(2014 Jun)

Fbw7 repression by hes5 creates a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions.
Sancho R, Blake SM, Tendeng C, Clurman BE, Lewis J, Behrens A
PLoS biology11(6)e1001586(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HES5 might contribute to the proliferation of non-small cell lung cancer by STAT3 signaling.
Title: HES5 promotes cellular proliferation of non-small cell lung cancer through STAT3 signaling.

that suppression of the HES5 leading to inhibition of proliferation may be one of the mechanisms against Hepatocellular carcinoma
Title: HES5 promotes cell proliferation and invasion through activation of STAT3 and predicts poor survival in hepatocellular carcinoma.

HES5 silencing is an early and recurrent change in prostate tumourigenesis
Title: HES5 silencing is an early and recurrent change in prostate tumourigenesis.

these data identify HES5 as a key mediator of the Wnt-3a proneurogenic effect occurring independently of the classical Wnt/beta-catenin signaling cascade thus further deciphering crosstalk mechanisms of Wnt and Notch signaling pathways regulating cell fate
Title: HES5 is a key mediator of Wnt-3a-induced neuronal differentiation.

the downstream Notch signalling effector HES5 directly represses transcription of the E3 ligase Fbw7beta.
Title: Fbw7 repression by hes5 creates a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions.

The following HES5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HES5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29131	NM_001010926.4 Latest version!	Homo sapiens hes family bHLH transcription factor 5 (HES5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu33432	XM_005244751.4 Latest version!	Homo sapiens hes family bHLH transcription factor 5 (HES5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu29131	NM_001010926.3	Homo sapiens hes family bHLH transcription factor 5 (HES5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HES5 ( NM_001010926.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001010926.1 Homo sapiens hes family bHLH transcription factor 5 (HES5), mRNA.

Price & Availability↑

CloneID	OHu29131
Clone ID Related Accession (Same CDS sequence)	NM_001010926.4 , NM_001010926.3
Accession Version	NM_001010926.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	transcription factor HES-5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139246.21, DQ272660.1 and BX104111.1. On Nov 23, 2018 this sequence version replaced NM_001010926.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ272660.1, HY107217.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000378453.4/ ENSP00000367714.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCCCA GCACTGTGGC CGTGGAGCTG CTCAGCCCCA AAGAGAAAAA CCGACTGCGG 
AAGCCGGTGG TGGAGAAGAT GCGCCGCGAC CGCATCAACA GCAGCATCGA GCAGCTGAAG 
CTGCTGCTGG AGCAGGAGTT CGCGCGGCAC CAGCCCAACT CCAAGCTGGA GAAGGCCGAC 
ATCCTGGAGA TGGCTGTCAG CTACCTGAAG CACAGCAAAG CCTTCGTCGC CGCCGCCGGC 
CCCAAGAGCC TGCACCAGGA CTACAGCGAA GGCTACTCGT GGTGCCTGCA GGAGGCCGTG 
CAGTTCCTGA CGCTCCACGC CGCCAGCGAC ACGCAGATGA AGCTGCTGTA CCACTTCCAG 
CGGCCCCCGG CCGCGCCCGC CGCGCCCGCC AAGGAGCCCA AGGCGCCGGG CGCCGCGCCC 
CCGCCCGCGC TCTCCGCCAA GGCCACCGCC GCCGCCGCCG CCGCGCACCA GCCCGCCTGC 
GGCCTCTGGC GGCCCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001010926.1
CDS	100..600
Translation	MAPSTVAVELLSPKEKNRLRKPVVEKMRRDRINSSIEQLKLLLEQEFARHQPNSKLEKADILEMAVSYLKHSKAFVAAAGPKSLHQDYSEGYSWCLQEAVQFLTLHAASDTQMKLLYHFQRPPAAPAAPAKEPKAPGAAPPPALSAKATAAAAAAHQPACGLWRPW

Target ORF information:

RefSeq Version	NM_001010926.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hes family bHLH transcription factor 5 (HES5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001010926.4

ORF Insert Sequence:

ATGGCCCCCA GCACTGTGGC CGTGGAGCTG CTCAGCCCCA AAGAGAAAAA CCGACTGCGG 
AAGCCGGTGG TGGAGAAGAT GCGCCGCGAC CGCATCAACA GCAGCATCGA GCAGCTGAAG 
CTGCTGCTGG AGCAGGAGTT CGCGCGGCAC CAGCCCAACT CCAAGCTGGA GAAGGCCGAC 
ATCCTGGAGA TGGCTGTCAG CTACCTGAAG CACAGCAAAG CCTTCGTCGC CGCCGCCGGC 
CCCAAGAGCC TGCACCAGGA CTACAGCGAA GGCTACTCGT GGTGCCTGCA GGAGGCCGTG 
CAGTTCCTGA CGCTCCACGC CGCCAGCGAC ACGCAGATGA AGCTGCTGTA CCACTTCCAG 
CGGCCCCCGG CCGCGCCCGC CGCGCCCGCC AAGGAGCCCA AGGCGCCGGG CGCCGCGCCC 
CCGCCCGCGC TCTCCGCCAA GGCCACCGCC GCCGCCGCCG CCGCGCACCA GCCCGCCTGC 
GGCCTCTGGC GGCCCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HES5 ( NM_001010926.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001010926.1 Homo sapiens hes family bHLH transcription factor 5 (HES5), mRNA.

Price & Availability↑

CloneID	OHu29131
Clone ID Related Accession (Same CDS sequence)	NM_001010926.4 , NM_001010926.3
Accession Version	NM_001010926.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	transcription factor HES-5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ272660.1 and BX104111.1. On Dec 24, 2008 this sequence version replaced NM_001010926.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ272660.1, HY107217.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCCA GCACTGTGGC CGTGGAGCTG CTCAGCCCCA AAGAGAAAAA CCGACTGCGG 
AAGCCGGTGG TGGAGAAGAT GCGCCGCGAC CGCATCAACA GCAGCATCGA GCAGCTGAAG 
CTGCTGCTGG AGCAGGAGTT CGCGCGGCAC CAGCCCAACT CCAAGCTGGA GAAGGCCGAC 
ATCCTGGAGA TGGCTGTCAG CTACCTGAAG CACAGCAAAG CCTTCGTCGC CGCCGCCGGC 
CCCAAGAGCC TGCACCAGGA CTACAGCGAA GGCTACTCGT GGTGCCTGCA GGAGGCCGTG 
CAGTTCCTGA CGCTCCACGC CGCCAGCGAC ACGCAGATGA AGCTGCTGTA CCACTTCCAG 
CGGCCCCCGG CCGCGCCCGC CGCGCCCGCC AAGGAGCCCA AGGCGCCGGG CGCCGCGCCC 
CCGCCCGCGC TCTCCGCCAA GGCCACCGCC GCCGCCGCCG CCGCGCACCA GCCCGCCTGC 
GGCCTCTGGC GGCCCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001010926.1
CDS	82..582
Translation	MAPSTVAVELLSPKEKNRLRKPVVEKMRRDRINSSIEQLKLLLEQEFARHQPNSKLEKADILEMAVSYLKHSKAFVAAAGPKSLHQDYSEGYSWCLQEAVQFLTLHAASDTQMKLLYHFQRPPAAPAAPAKEPKAPGAAPPPALSAKATAAAAAAHQPACGLWRPW

Target ORF information:

RefSeq Version	NM_001010926.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hes family bHLH transcription factor 5 (HES5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001010926.3

ORF Insert Sequence:

ATGGCCCCCA GCACTGTGGC CGTGGAGCTG CTCAGCCCCA AAGAGAAAAA CCGACTGCGG 
AAGCCGGTGG TGGAGAAGAT GCGCCGCGAC CGCATCAACA GCAGCATCGA GCAGCTGAAG 
CTGCTGCTGG AGCAGGAGTT CGCGCGGCAC CAGCCCAACT CCAAGCTGGA GAAGGCCGAC 
ATCCTGGAGA TGGCTGTCAG CTACCTGAAG CACAGCAAAG CCTTCGTCGC CGCCGCCGGC 
CCCAAGAGCC TGCACCAGGA CTACAGCGAA GGCTACTCGT GGTGCCTGCA GGAGGCCGTG 
CAGTTCCTGA CGCTCCACGC CGCCAGCGAC ACGCAGATGA AGCTGCTGTA CCACTTCCAG 
CGGCCCCCGG CCGCGCCCGC CGCGCCCGCC AAGGAGCCCA AGGCGCCGGG CGCCGCGCCC 
CCGCCCGCGC TCTCCGCCAA GGCCACCGCC GCCGCCGCCG CCGCGCACCA GCCCGCCTGC 
GGCCTCTGGC GGCCCTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HES5 ( XM_005244751.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005244808.1 Homo sapiens hes family bHLH transcription factor 5 (HES5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu33432
Clone ID Related Accession (Same CDS sequence)	XM_005244751.4
Accession Version	XM_005244751.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcription factor HES-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005244751.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCCA GCACTGTGGC CGTGGAGCTG CTCAGCCCCA AAGAGAAAAA CCGACTGCGG 
AAGCCGGTGG TGGAGAAGAT GCGCCGCGAC CGCATCAACA GCAGCATCGA GCAGCTGAAG 
CTGCTGCTGG AGCAGGAGTT CGCGCGGCAC CAGCCCAACT CCAAGCTGGA GAAGGCCGAC 
ATCCTGGAGA TGGCTGTCAG CTACCTGAAG CACAGCAAAG GTGAGCGCGC CCGGGCCCCC 
CGCGCCCCGA GTTCCCACCG CGCCCCGGCT CCCCCGCGCC CCGCCGCCCG CTCACCGCCG 
CCGCGTCTCC CCGCAGCCTT CGTCGCCGCC GCCGGCCCCA AGAGCCTGCA CCAGGACTAC 
AGCGAAGGCT ACTCGTGGTG CCTGCAGGAG GCCGTGCAGT TCCTGACGCT CCACGCCGCC 
AGCGACACGC AGATGAAGCT GCTGTACCAC TTCCAGCGGC CCCCGGCCGC GCCCGCCGCG 
CCCGCCAAGG AGCCCAAGGC GCCGGGCGCC GCGCCCCCGC CCGCGCTCTC CGCCAAGGCC 
ACCGCCGCCG CCGCCGCCGC GCACCAGCCC GCCTGCGGCC TCTGGCGGCC CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005244808.1
CDS	391..987
Translation	MAPSTVAVELLSPKEKNRLRKPVVEKMRRDRINSSIEQLKLLLEQEFARHQPNSKLEKADILEMAVSYLKHSKGERARAPRAPSSHRAPAPPRPAARSPPPRLPAAFVAAAGPKSLHQDYSEGYSWCLQEAVQFLTLHAASDTQMKLLYHFQRPPAAPAAPAKEPKAPGAAPPPALSAKATAAAAAAHQPACGLWRPW

Target ORF information:

RefSeq Version	XM_005244751.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hes family bHLH transcription factor 5 (HES5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005244751.4

ORF Insert Sequence:

ATGGCCCCCA GCACTGTGGC CGTGGAGCTG CTCAGCCCCA AAGAGAAAAA CCGACTGCGG 
AAGCCGGTGG TGGAGAAGAT GCGCCGCGAC CGCATCAACA GCAGCATCGA GCAGCTGAAG 
CTGCTGCTGG AGCAGGAGTT CGCGCGGCAC CAGCCCAACT CCAAGCTGGA GAAGGCCGAC 
ATCCTGGAGA TGGCTGTCAG CTACCTGAAG CACAGCAAAG GTGAGCGCGC CCGGGCCCCC 
CGCGCCCCGA GTTCCCACCG CGCCCCGGCT CCCCCGCGCC CCGCCGCCCG CTCACCGCCG 
CCGCGTCTCC CCGCAGCCTT CGTCGCCGCC GCCGGCCCCA AGAGCCTGCA CCAGGACTAC 
AGCGAAGGCT ACTCGTGGTG CCTGCAGGAG GCCGTGCAGT TCCTGACGCT CCACGCCGCC 
AGCGACACGC AGATGAAGCT GCTGTACCAC TTCCAGCGGC CCCCGGCCGC GCCCGCCGCG 
CCCGCCAAGG AGCCCAAGGC GCCGGGCGCC GCGCCCCCGC CCGCGCTCTC CGCCAAGGCC 
ACCGCCGCCG CCGCCGCCGC GCACCAGCCC GCCTGCGGCC TCTGGCGGCC CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HES5 promotes cellular proliferation of non-small cell lung cancer through STAT3 signaling.
Oncology reports37(1)474-482(2017 Jan)
Gu S,Zhang R,Gu J,Li X,Lv L,Jiang J,Xu Z,Wang S,Shi C,Wang DP,Wu C

HES5 promotes cell proliferation and invasion through activation of STAT3 and predicts poor survival in hepatocellular carcinoma.
Experimental and molecular pathology99(3)474-84(2015 Dec)
Zhu G,Shi W,Fan H,Zhang X,Xu J,Chen Y,Xu Z,Tao T,Cheng C

HES5 silencing is an early and recurrent change in prostate tumourigenesis.
Endocrine-related cancer22(2)131-44(2015 Apr)
Massie CE,Spiteri I,Ross-Adams H,Luxton H,Kay J,Whitaker HC,Dunning MJ,Lamb AD,Ramos-Montoya A,Brewer DS,Cooper CS,Eeles R,,Warren AY,Tavar? S,Neal DE,Lynch AG

HES5 is a key mediator of Wnt-3a-induced neuronal differentiation.
Stem cells and development23(12)1328-39(2014 Jun)
Mu?mann C,H?bner R,Trilck M,Rolfs A,Frech MJ

Fbw7 repression by hes5 creates a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions.
PLoS biology11(6)e1001586(2013)
Sancho R,Blake SM,Tendeng C,Clurman BE,Lewis J,Behrens A

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HES5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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